High-flux bioreactor gas supply device with independent flow control and proportion adjustment

Abstract

The utility model relates to the technical field of mixed gas supply of a bioreactor, in particular to a high-flux bioreactor gas supply device with independent flow control and proportion regulation, which comprises a gas flow meter, a busbar bottom plate and a plurality of electromagnetic regulating valves, the multiple electromagnetic regulating valves are all arranged on the busbar bottom plate and located above the busbar bottom plate, and the busbar bottom plate is provided with an air inlet channel, an oxygen inlet channel, a carbon dioxide inlet channel and a plurality of air outlet channels. The air inlet channel, the oxygen inlet channel and the carbon dioxide inlet channel are connected with air inlets of the corresponding electromagnetic regulating valves respectively, and the multiple air outlet channels are connected with air outlets of the corresponding electromagnetic regulating valves respectively. In this way, the technical problems that in the prior art, during gas supply of a high-flux bioreactor, automatic flow control cannot be achieved, automatic proportion adjustment cannot be achieved, the occupied space of the device is large, and cost is too high are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of mixed gas supply for bioreactors, in particular to a high-throughput bioreactor gas supply device with independent flow control and ratio regulation. Background Art

[0002] The bioreactor gas supply device can ensure the supply of oxygen and carbon dioxide required for cell growth in the bioreactor, thereby maintaining cell growth and avoiding the inhibition of cell growth and bacterial fermentation expression by excessively high or low dissolved oxygen and pH. It is an extremely important component of the bioreactor system.

[0003] Most of the current bioreactor gas supply devices use a combination of a float flowmeter + a gas flow controller to achieve gas flow control and ratio adjustment, but this combination becomes inapplicable to high-throughput bioreactors. Although the float flowmeter is economical, the operation cannot be automated and will cause large errors, making parallel comparison impossible. The large size and high unit price of the gas flow controller limit its application in high-throughput bioreactors, resulting in the inability of the high-throughput bioreactor system to fully play its role in clone screening and process optimization, thereby limiting the application of the high-throughput bioreactor system. Utility Model Content

[0004] The utility model aims to provide a high-throughput bioreactor gas supply device with independent flow control and proportional regulation, aiming to solve the technical problems in the prior art of high-throughput bioreactor gas supply that cannot automatically control the flow, cannot automatically adjust the proportion, the device occupies a large space, and the cost is too high.

[0005] To achieve the above-mentioned purpose, the utility model adopts a high-throughput bioreactor gas supply device with independent flow control and ratio regulation, including a gas flow meter, a busbar bottom plate and a plurality of electromagnetic regulating valves, wherein the gas flow meter is connected to the busbar bottom plate, the plurality of electromagnetic regulating valves are arranged on the busbar bottom plate, and the plurality of electromagnetic regulating valves are located above the busbar bottom plate, the busbar bottom plate is provided with an air inlet channel, an oxygen inlet channel, a carbon dioxide inlet channel and a plurality of outlet channels, the air inlet channel, the oxygen inlet channel and the carbon dioxide inlet channel are respectively connected to the corresponding air inlets of the electromagnetic regulating valves, and the plurality of outlet channels are respectively connected to the corresponding air outlets of the electromagnetic regulating valves;

[0006] There are multiple gas flow meters, and the multiple gas flow meters are respectively connected to the corresponding gas outlet channels.

[0007] The electromagnetic regulating valve comprises a regulating valve body and two mounting blocks, the two mounting blocks are respectively in contact with the busbar bottom plate, the regulating valve body is fixedly connected to the two mounting blocks, and two adjacent mounting blocks are staggered.

[0008] Wherein, the mounting block is provided with a mounting hole.

[0009] Wherein, the busbar bottom plate is in a rectangular parallelepiped shape.

[0010] Wherein, the plurality of gas flow meters are all located on the same side of the busbar bottom plate.

[0011] The utility model discloses a high-throughput bioreactor gas supply device with independent flow control and ratio regulation. In specific use, the air inlet channel, the oxygen inlet channel and the carbon dioxide inlet channel are respectively connected to the external air cylinder, oxygen cylinder and carbon dioxide cylinder, and the gas outlet of the electromagnetic regulating valve is merged through the bus bottom plate to achieve the mixing of air, oxygen and carbon dioxide. Then, the gas flow meter is connected to the gas outlet of the mixing chamber, and is connected to the micro bioreactor through a one-way valve to achieve mixed gas supply with controllable flow rate and gas ratio. The electromagnetic regulating valve has the function of controlling the valve opening by controlling the current size, and can independently and automatically control the opening of each valve by external signal input. The meter has the function of outputting analog signals. The output signal is converted into an electric analog signal and then displayed on the display screen. The signal can also be output to the control board. The control board dynamically adjusts the opening of the carbon dioxide solenoid valve according to the external pH signal, so as to maintain the pH of the fermentation liquid in the bioreactor. The utility model can realize independent automatic air intake control of each channel of the bioreactor by independently controlling the gas flow and mixing ratio of each channel, thereby realizing automatic pH and DO dissolved oxygen control, avoiding the problems of traditional float flowmeters such as non-automaticity, low precision, and non-parallelism, and solving the technical problems of the inability to automatically control the flow, the inability to automatically adjust the ratio, the large space occupied by the device, and the high cost in the gas supply of high-throughput bioreactors in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0013] Figure 1 The utility model is a schematic diagram of the structure of a high-throughput bioreactor gas supply device with independent flow control and ratio regulation.

[0014] Figure 2 It is a front view of the high-throughput bioreactor gas supply device with independent flow control and ratio regulation of the utility model.

[0015] Figure 3 It is a side view of a high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to the utility model.

[0016] 101 - gas flow meter, 102 - bus bar bottom plate, 103 - regulating valve body, 104 - mounting block, 105 - air inlet channel, 106 - oxygen inlet channel, 107 - carbon dioxide inlet channel, 108 - outlet channel, 109 - mounting hole. DETAILED DESCRIPTION

[0017] Embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0018] See also Figure 1 to Figure 3 ,in Figure 1 This is a schematic diagram of the structure of the high-throughput bioreactor gas supply device with independent flow control and ratio regulation of the utility model. Figure 2 This is the front view of the high-throughput bioreactor gas supply device with independent flow control and ratio regulation of the utility model. Figure 3 It is a side view of a high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to the utility model.

[0019] The utility model provides a high-throughput bioreactor gas supply device with independent flow control and ratio regulation, comprising a gas flow meter 101, a busbar bottom plate 102 and a plurality of electromagnetic regulating valves, wherein the gas flow meter 101 is connected to the busbar bottom plate 102, the plurality of electromagnetic regulating valves are arranged on the busbar bottom plate 102, and the plurality of electromagnetic regulating valves are located above the busbar bottom plate 102, the busbar bottom plate 102 is provided with an air inlet channel 105, an oxygen inlet channel 106, a carbon dioxide inlet channel 107 and a plurality of gas outlet channels 108, the air inlet channel 105, the oxygen inlet channel 106 and the carbon dioxide inlet channel 107 are respectively connected to the gas inlets of the corresponding electromagnetic regulating valves, and the plurality of gas outlet channels 108 are respectively connected to the gas outlets of the corresponding electromagnetic regulating valves;

[0020] There are multiple gas flow meters 101, and the multiple gas flow meters 101 are respectively connected to the corresponding gas outlet channels 108;

[0021] The busbar bottom plate 102 is in a rectangular parallelepiped shape;

[0022] The plurality of gas flow meters 101 are all located on the same side of the busbar bottom plate 102 .

[0023] According to this specific embodiment, a plurality of the electromagnetic regulating valves are fixed on the bus bottom plate 102, wherein the air inlets of the first row of the electromagnetic regulating valves are communicated with the air inlet channel 105, the air inlets of the second row of the electromagnetic valves are communicated with the oxygen inlet channel 106, the air inlets of the third row of the electromagnetic valves are connected with the carbon dioxide inlet channel 107, the air outlets of the first column of the electromagnetic valves are connected with the corresponding air outlet channels 108, connected to the first group of gas flow meters 101, and then connected to the bubble generator of the first bioreactor culture tank, the gas flow meter 101 is used to detect the flow rate of the outlet gas, the electromagnetic regulating valve is used to realize the ratio adjustment of the flow rate and the mixed gas, the bus bottom plate 102 is used to summarize the gas supply and outlet of the electromagnetic regulating valves, and the summarized air outlet is connected to the gas flow meter 101, so as to realize the gas supply to the gas flow meter 101.

[0024] The solenoid regulating valve comprises a regulating valve body 103 and two mounting blocks 104, the two mounting blocks 104 are respectively in contact with the busbar bottom plate 102, and the regulating valve body 103 is fixedly connected to the two mounting blocks 104, and the two adjacent mounting blocks 104 are staggered.

[0025] The mounting block 104 has a mounting hole 109 .

[0026] According to this specific embodiment, the corresponding solenoid valve body can be installed and fixed by fixing the two mounting blocks 104 on the bus base plate 102 through the two mounting holes 109, and the two mounting blocks 104 are of different shapes, so that two adjacent mounting blocks 104 can be staggered, thereby saving installation space.

[0027] The utility model is used to provide a high-throughput bioreactor gas supply device with independent flow control and ratio adjustment. When in use, the air inlet channel 105, the oxygen inlet channel 106 and the carbon dioxide inlet channel 107 are respectively connected to the external air cylinder, oxygen cylinder and carbon dioxide cylinder, and the gas outlet of the electromagnetic regulating valve is merged through the bus bottom plate 102 to achieve the mixing of air, oxygen and carbon dioxide, and then the gas flow meter 101 is connected to the gas outlet of the mixing chamber, and then connected to the micro bioreactor through a one-way valve, so as to achieve mixed gas supply with controllable flow and gas ratio. The electromagnetic regulating valve has the function of controlling the valve opening by controlling the current size, and can independently and automatically control the opening of each valve through external signal input. The gas flowmeter 101 has the function of outputting analog signals. The output signal is converted into an electric analog signal and displayed on the display screen. The signal can also be output to the control board. The control board dynamically adjusts the opening of the carbon dioxide solenoid valve according to the external pH signal, so as to maintain the pH of the fermentation liquid in the bioreactor. The utility model can realize independent automatic air intake control of each channel of the bioreactor by independently controlling the gas flow and mixing ratio of each channel, thereby realizing automatic pH and DO dissolved oxygen control, avoiding the problems of traditional float flowmeters such as non-automatic, low precision, and non-parallelism, and solving the technical problems of the inability to automatically control the flow, the inability to automatically adjust the ratio, the large space occupied by the device, and the high cost in the gas supply of high-throughput bioreactors in the prior art.

[0028] The above disclosure is only a preferred embodiment of the present invention, and certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope covered by the utility model.

Claims

1. A high-throughput bioreactor gas supply device with independent flow control and ratio regulation, characterized in that: It comprises a gas flow meter, a busbar bottom plate and a plurality of electromagnetic regulating valves, wherein the gas flow meter is connected to the busbar bottom plate, the plurality of electromagnetic regulating valves are arranged on the busbar bottom plate, and the plurality of electromagnetic regulating valves are located above the busbar bottom plate, the busbar bottom plate is provided with an air inlet channel, an oxygen inlet channel, a carbon dioxide inlet channel and a plurality of outlet channels, the air inlet channel, the oxygen inlet channel and the carbon dioxide inlet channel are respectively connected to the air inlets of the corresponding electromagnetic regulating valves, and the plurality of outlet channels are respectively connected to the air outlets of the corresponding electromagnetic regulating valves; There are multiple gas flow meters, and the multiple gas flow meters are respectively connected to the corresponding gas outlet channels.

2. The high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to claim 1, characterized in that: The electromagnetic regulating valve comprises a regulating valve body and two mounting blocks, the two mounting blocks are respectively in contact with the busbar bottom plate, and the regulating valve body is fixedly connected to the two mounting blocks, and two adjacent mounting blocks are staggered.

3. The high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to claim 2, characterized in that: The mounting block is provided with a mounting hole.

4. The high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to claim 3, characterized in that: The busbar bottom plate is in a rectangular parallelepiped shape.

5. The high-throughput bioreactor gas supply device with independent flow control and ratio regulation according to claim 4, characterized in that: The plurality of gas flow meters are all located on the same side of the busbar bottom plate.

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