Full-automatic liquid-based cell staining system

Abstract

The utility model discloses a full-automatic liquid-based cell staining system, which relates to the technical field of cell staining and comprises a ventilation box, and a slide movement mechanism, a slide frame, a plurality of staining cabins and a temperature control module are arranged in the ventilation box. The slide movement mechanism comprises a horizontal movement device and a lifting device, and the horizontal movement device comprises a transverse movement guide rail; the dyeing cabins are uniformly arranged at the bottom of the ventilation box and are used for accommodating dyeing reagents; the slide rack bears a to-be-dyed slide, the top of the slide rack is connected with the lifting device, the lifting device is slidably connected with the transverse movement guide rail, and the transverse movement guide rail is parallel to the dyeing cabins; the temperature control module comprises a temperature sensor and a constant-temperature controller, monitors and adjusts the temperature in the ventilation box in real time, and ensures that the internal temperature is always the optimum temperature of the dyeing reagent; while the automation of the dyeing process is realized, the temperature in the ventilation box is monitored and adjusted in real time through the temperature control module in the dyeing process, so that the dyeing efficiency and the dyeing quality of the dyeing reagent are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell staining, in particular to a full-automatic liquid-based cell staining system. Background Art

[0002] In medical testing and biological research, in order to better observe cell samples, it is often necessary to stain them. Liquid-based cell staining is a common clinical detection method. For example, liquid-based cervical cell staining can be used to detect cervical precancerous lesions and cervical cancer at an early stage.

[0003] The traditional staining process requires manual operation, which may lead to the following problems: First, the operator's technical level and operational standardization may affect the consistency and accuracy of staining. Different operators may have differences, resulting in reduced comparability of staining results. Second, human factors may lead to operational errors and instability, such as the amount of staining reagents added, the control of staining time, etc. These human factors may introduce errors and affect the staining quality and the reliability of the results.

[0004] In recent years, some liquid-based automatic cell staining instruments have appeared, but these devices are often complex in structure and cumbersome in operation. In addition, since the reaction rate and staining effect of the staining agent are affected by the ambient temperature, the staining quality of existing instruments is not ideal and the accuracy and consistency of cell staining cannot be guaranteed. Utility Model Content

[0005] In view of the deficiencies in the above-mentioned prior art, the utility model provides a fully automatic liquid-based cell staining system, which can realize fully automated operation of liquid-based cell staining. At the same time, through precise temperature control, it ensures that the staining reagents achieve the best effect, effectively improves the staining efficiency as well as the accuracy and reliability of staining, which is conducive to improving the accuracy of cervical disease diagnosis.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A fully automatic liquid-based cell staining system comprises a ventilation box, wherein a slide movement mechanism, a slide rack, a plurality of staining chambers and a temperature control module are arranged in the ventilation box; the slide movement mechanism comprises a horizontal movement device and a lifting device, and the horizontal movement device comprises a transverse movement guide rail;

[0008] The staining cabins are arranged evenly at the bottom of the ventilation box and are used to accommodate staining reagents; the slide rack carries the slides to be stained, the top of the slide rack is connected to a lifting device, the lifting device is slidably connected to a transverse motion guide rail, and the transverse motion guide rail is arranged in parallel with a plurality of staining cabins;

[0009] The temperature control module includes a temperature sensor and a constant temperature controller, which monitors and adjusts the temperature inside the ventilation box in real time to ensure that the internal temperature is always at the most suitable temperature for the dyeing reagent.

[0010] As a further implementation, the temperature control modules are provided in plurality and are arranged at the inlet, outlet and middle position of the ventilation box respectively.

[0011] As a further implementation, the slide rack is a metal structure, and the slides to be stained are placed on the slide rack to prevent the slide rack from reacting with the staining reagent and affecting the staining effect.

[0012] As a further implementation, the number of the staining chambers matches the type of staining reagent.

[0013] As a further implementation, the staining reagent includes BS fixative, 5N hydrochloric acid, DNA staining solution, 50% alcohol, 75% alcohol, 95% alcohol, 100% alcohol, and xylene solvent.

[0014] As a further implementation, each stain is followed by clean water for washing to remove excess stain and other impurities.

[0015] As a further implementation, a linear drive motor is arranged in the lateral motion guide rail, and the linear drive motor is connected to the lifting device through a coupling and a lead screw.

[0016] As a further implementation, a plurality of limit switches are arranged in the transverse motion guide rail, and the limit switches correspond to the position of each dyeing cabin respectively.

[0017] As a further implementation, the lifting device includes a mechanical gripper, the upper portion of the mechanical gripper is connected to a lifting rod, and the lifting rod is driven by a motor.

[0018] As a further implementation method, a PLC controller is further provided in the ventilation box, and the PLC controller is connected to the glass slide movement mechanism and the temperature control module respectively.

[0019] By adopting the above technical solution, the beneficial effects of the utility model are as follows:

[0020] 1. Considering that temperature is an important factor affecting the efficiency and effect of cell staining, the utility model can realize the automated operation of the staining process while arranging a temperature control module to accurately control the temperature in the liquid-based cell staining process, thereby ensuring that the staining reagent functions normally, thereby providing efficient and accurate staining results, improving the staining quality and the accuracy of diagnosis, providing doctors and researchers with more reliable cytological data, and promoting the early diagnosis and treatment of cervical diseases.

[0021] 2. The utility model transforms traditional manual staining into a mechanized process operation through structures such as a staining cabin, a slide movement mechanism, a slide rack and a PLC controller, which significantly improves work efficiency, saves a lot of time and human resources, and improves the standardization and consistency of the staining process, which can reduce the impact of human factors on the staining results and further ensure the accuracy of staining.

[0022] 3. The devices and reagents required for dyeing in the utility model are all contained in the ventilation box, and the ventilation box is a closed structure, which can be sealed to avoid light and ensure that the dyeing reagents can function normally.

[0023] 4. The utility model has a compact design, reasonable structure, small footprint and is suitable for laboratory environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention.

[0025] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0026] Figure 2 It is the front view of the overall structure of the utility model;

[0027] Figure 3 This is a schematic diagram of the slide motion mechanism of the utility model;

[0028] Figure 4 This is a schematic diagram of the structure of the dyeing cabin of the utility model;

[0029] Figure 5 It is a structural schematic diagram of the glass slide rack of the utility model.

[0030] In the figure: 1. ventilation box; 2. horizontal moving device; 3. lifting device; 4. slide rack; 5. staining cabin; 6. temperature control module; 7. display screen; 8. switch;

[0031] 21. lateral motion guide rail; 22. linear motion motor; 31. mechanical gripper; 32. driving mechanism; 5a. BS fixative; 5b. 5N hydrochloric acid; 5c. DNA staining solution; 5d. 50% alcohol; 5e. 75% alcohol; 5f. 95% alcohol; 5g. 100% alcohol; 5h. xylene solvent; 5i. clean water. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the utility model. Unless otherwise specified, all technical and scientific terms used in the utility model have the same meanings as those commonly understood by ordinary technicians in the technical field to which the utility model belongs. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0033] Embodiment 1

[0034] In a typical embodiment of the present application, a fully automatic liquid-based cell staining system is provided, such as Figure 1-5 As shown, it includes a ventilation box 1, in which a slide movement mechanism, a slide rack 4, a plurality of staining chambers 5 and a temperature control module 6 are arranged; wherein the slide movement mechanism includes a horizontal moving device 2 and a lifting device 3, and the horizontal moving device 2 includes a transverse motion guide rail 21. A plurality of staining chambers 5 are arranged in a straight line and evenly arranged at the bottom of the ventilation box 1, and are used to contain staining reagents; the slide rack 4 carries the slides to be stained, and the top of the slide rack 4 is connected to the lifting device 3, and the lifting device 3 is slidably connected to the transverse motion guide rail 21, and the transverse motion guide rail 21 is arranged in parallel with the plurality of staining chambers 5 and fixedly connected to the ventilation box 1 at both ends; the lifting device can drive the slide rack to move horizontally along the transverse motion guide rail, and send the slides in the slide rack into different staining chambers in turn and immerse them in the staining reagents.

[0035] The temperature control module 6 includes a temperature sensor for detecting the ambient temperature in the ventilation box and a thermostat for adjusting the temperature. The thermostat can be a microcomputer temperature controller XH-W3002, with a temperature control range of -50°C to 110°C, which can meet the temperature requirements of the hydraulic cervical cell stain. Of course, other products on the market can also be used as needed. The temperature sensor and the thermostat are connected to the PLC controller, which can monitor and automatically adjust the temperature in the ventilation box in real time to ensure that the internal temperature is always at the most suitable temperature for the staining reagent, thereby improving the quality and consistency of the stained slides. A display screen 7 is provided on the outside of the ventilation box 1 to display the temperature in the ventilation box for easy observation.

[0036] Considering that the cell ventilation box has a certain volume and different temperature changes may occur at different positions during the staining process, the present application sets multiple temperature control modules at different positions to detect and control the temperature of different areas separately. As an optional implementation, three temperature control modules are set, which are arranged at the inlet, outlet and middle position between the inlet and outlet of the ventilation box respectively, so as to achieve constant temperature control in the entire ventilation box.

[0037] like Figure 1-2As shown, the lateral motion guide rail 21 is installed above the staining cabin 5 in the ventilation box, and a linear drive motor 22 is arranged in the lateral motion guide rail 21. The linear drive motor 22 is connected to the lifting device 3 through a coupling and a screw rod to drive the lifting device to slide horizontally along the guide rail to move the slide rack to the position of different staining cabins. Figure 2-3 As shown, the lifting device 3 includes a mechanical gripper 31 for clamping and fixing the slide rack, a driving mechanism 32 is provided above the mechanical gripper 31, and the driving mechanism is connected to the mechanical gripper through a lifting rod below. The driving mechanism adopts a motor and a screw transmission, which can drive the lifting rod to move linearly in the height direction, thereby controlling the lifting and lowering of the slide, immersing the slide in the staining reagents of each staining chamber in turn, and taking the slide out of the staining chamber and sending it to the next link after all the reagents are processed. In addition, when the slide is immersed in the staining chamber after descending, the lifting device can form a closed space with the staining chamber, which meets the light-shielding conditions for the reaction between the staining reagent and the cell slide, which is conducive to improving the staining quality.

[0038] Cell staining usually requires multiple staining agents to achieve, such as Figure 5 As shown, the staining reagents used in this embodiment include BS fixative 5a, 5N hydrochloric acid 5b, DNA staining solution 5c, 50% alcohol 5d, 75% alcohol 5e, 95% alcohol 5f, 100% alcohol 5g, and xylene solvent 5h. The number of staining chambers matches the type of staining reagents, and multiple staining chambers are arranged in a straight line, parallel to the transverse motion guide rail. In addition, in order to improve the staining effect, another staining chamber with the same number of staining agents is set to place clean water, and clean water 5i is placed after each staining reagent, so that the glass slide is washed with clean water immediately after each staining reagent immersion, which is used to remove excess staining reagents and other impurities. This can avoid the staining reagents remaining on the cells and interfere with the observation of cell morphology and structure, and at the same time can ensure the accuracy and reliability of the staining effect. In addition, washing with clean water also facilitates the control of staining time and staining concentration, thereby obtaining better staining results.

[0039] like Figure 5 As shown, the slide rack 4 is a metal structure to prevent the slide rack from reacting with the staining reagent. The slide rack can evenly place multiple slides to be stained, with a certain gap between the slides, and can perform batch staining on cell slides, improve staining efficiency and ensure uniform staining.

[0040] In addition, if Figure 1As shown, a switch 8 is also provided on the outside of the ventilation box 1, and the switch 8 controls the start or stop of the entire device, which is located on the front of the box body for easy operation by the staff. A PLC controller is also provided in the ventilation box, and the PLC controller is connected to the linear motion motor, the drive mechanism and the temperature control module respectively. Among them, the descent and lifting of the glass slide rack can be controlled by the drive mechanism, and the glass slide rack is controlled to move to the top of each staining cabin by the linear motion motor. In order to improve the positioning accuracy, a limit switch is installed at the corresponding position above each staining cabin in the transverse motion guide rail. The temperature information in the ventilation box collected in real time by the temperature sensor is transmitted to the PLC controller. After the PLC control program and the target temperature are logically judged, the corresponding thermostat controller is controlled to heat up, cool down or remain unchanged, so as to achieve the internal temperature of the entire ventilation box to be always and constant, and always maintain the most suitable temperature for the staining reagent, which can improve the staining reaction rate. At the same time, the constant temperature can provide the repeatability of the experimental results. At different temperatures, the reaction rate and selectivity of the staining reagent may vary, resulting in differences in staining results. By maintaining a constant temperature, the variability between experiments can be reduced, making the results more consistent and comparable.

[0041] The above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. For those skilled in the art, the utility model may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A fully automatic liquid-based cell staining system, characterized in that: It comprises a ventilation box, in which a slide movement mechanism, a slide rack, a plurality of staining chambers and a temperature control module are arranged; the slide movement mechanism comprises a horizontal movement device and a lifting device, and the horizontal movement device comprises a transverse movement guide rail; The staining cabins are evenly arranged at the bottom of the ventilation box and are used to accommodate staining reagents; the slide rack carries the slides to be stained, the top of the slide rack is connected to a lifting device, the lifting device is slidably connected to a transverse motion guide rail, and the transverse motion guide rail is arranged in parallel with several staining cabins; the temperature control module includes a temperature sensor and a constant temperature controller.

2. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: The temperature control modules are provided in plurality and are arranged at the inlet, outlet and middle position of the ventilation box respectively.

3. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: The slide rack is a metal structure, and the slide to be stained is placed on the slide rack.

4. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: The number of the staining chambers matches the type of staining reagents.

5. A fully automatic liquid-based cell staining system as claimed in claim 4, characterized in that: The staining reagent includes BS fixative, 5N hydrochloric acid, DNA staining solution, 50% alcohol, 75% alcohol, 95% alcohol, 100% alcohol and xylene solvent.

6. A fully automatic liquid-based cell staining system as claimed in claim 5, characterized in that: After each stain, clean water is placed for washing.

7. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: A linear drive motor is arranged in the lateral motion guide rail, and the linear drive motor is connected to the lifting device through a coupling and a lead screw.

8. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: A plurality of limit switches are arranged in the transverse motion guide rail, and the limit switches correspond to the position of each dyeing cabin respectively.

9. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: The lifting device comprises a mechanical gripper, the upper part of the mechanical gripper is connected to a lifting rod, and the lifting rod is driven by a motor.

10. A fully automatic liquid-based cell staining system as claimed in claim 1, characterized in that: A PLC controller is also arranged in the ventilation box, and the PLC controller is connected to the slide movement mechanism and the temperature control module respectively.

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