Solid-phase extraction automatic sampling equipment
By introducing flow rate adjustment and negative pressure devices into the solid-phase extraction automatic sampling equipment, the problems of traditional manual solid-phase extraction time and low efficiency of automatic extraction devices are solved, and efficient treatment of large-volume water samples and repeatable experimental results are achieved. Sex improvement.
Patent Information
- Application Number
- CN202420479195.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-12
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2034-03-12
Smart Images
Figure CN222144660U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of water sample pretreatment devices, and in particular to a solid phase extraction automatic sampling device. Background Art
[0002] In the related technology, solid phase extraction is a technique that uses solid materials with adsorption properties to enrich and separate solutes from liquid mixtures. This method is usually used to extract and concentrate target substances in samples for subsequent analysis or processing. By using different types of solid phase extraction columns, adsorbents and elution solvents, highly selective and sensitive extraction of target compounds in different environmental samples can be achieved, including water, soil, air, biological tissues, etc. This makes it widely used in various fields such as environmental analysis, biomedical research, and food safety monitoring.
[0003] In view of the low content of organic pollutants in environmental water samples, drinking water and source water, solid phase extraction technology is commonly used to concentrate and enrich large-volume water samples. The operation steps generally include extraction column activation, sample loading, elution and other steps. As the rate-limiting step in the pretreatment of large-volume water samples, the traditional manual solid phase extraction method requires manual addition of sample solution and elution reagent to the solid phase extraction column one by one. The experimental process is cumbersome and time-consuming, especially when extracting large-volume water samples. It takes a long time, the extraction time is uncontrollable, and it is impossible to process large-volume water samples with high throughput. At the same time, this device can also meet the needs of batch pretreatment of large-volume water samples in (application number: CN202311069523.7), that is, the problem of cumbersome and time-consuming manual solid phase extraction sample addition process.
[0004] The traditional manual solid-phase extraction method requires manual addition of sample solution into the solid-phase extraction column one by one. When the volume of the water sample is too large, the loading time is long, and multiple samples cannot be processed at the same time, and the extraction time between different samples cannot be guaranteed to be consistent. The commercial large-volume automatic solid-phase extraction instrument still has the following problems: 1. The price is high, which limits its application; 2. The existing commercial large-volume automatic extraction device is limited by the instrument volume and can generally only process 4-6 water samples at the same time, with low processing throughput and low efficiency, and it is impossible to flexibly increase or reduce sample positions according to experimental needs; 3. The large-volume solid-phase extraction device that uses siphoning to add samples cannot adjust the flow rate and cannot control the solid-phase extraction time, so the parallelism of the experiment is poor, which reduces the repeatability of the experiment.
[0005] Regarding the large-volume water sample batch processing device / technology, the patent document with application number CN202311069523.7 discloses "A low-cost large-volume water sample semi-automatic siphon batch processing device", which introduces a solid phase extraction pretreatment device that uses siphon loading to achieve semi-automatic batch processing of large-volume water samples. Its main disadvantages are: 1. The large-volume sample bottles need to be placed in a special sample rack, which increases the cost. Not only does it have certain limitations in application, but the sample bottles placed at high places also have the potential risk of tipping over and water sample loss; 2. When using siphon loading, the flow rate cannot be adjusted and the solid phase extraction time cannot be controlled, resulting in poor parallelism of the experiment and reduced repeatability of the experiment. Utility Model Content
[0006] The present application aims to solve at least one of the technical problems existing in the prior art. To this end, the present application proposes a solid phase extraction automatic sampling device that can adjust the liquid flow rate and control the extraction time to effectively improve the probability of reproducibility of experimental results.
[0007] The solid phase extraction automatic sampling device of the embodiment of the present application comprises:
[0008] A solid-liquid extraction device, comprising an extraction device body, a liquid inlet connection end and a negative pressure connection end, wherein the extraction device body has a collection chamber, the liquid inlet connection end is mounted on the extraction device body and communicates with the collection chamber, and the negative pressure connection end is mounted on the extraction device body and communicates with the collection chamber;
[0009] A delivery assembly, comprising a first pipeline, a solid phase extraction column and a flow rate regulating device, wherein the liquid inlet end of the solid phase extraction column is connected to one end of the first pipeline, the flow rate regulating device is used to regulate the flow rate of the fluid in the first pipeline, and the output end of the solid phase extraction column is connected to the liquid inlet connection end;
[0010] A negative pressure device is connected to the negative pressure connection end.
[0011] Furthermore, there are a plurality of liquid inlet connection ends, and the plurality of liquid inlet connection ends are arranged at intervals on the extraction device body.
[0012] Furthermore, the flow rate regulating device includes an infusion regulator or a flow regulating valve.
[0013] Furthermore, the infusion regulator includes a base and an adjusting knob, the base is provided with a channel for accommodating a first pipe, the adjusting knob is mounted on the base, and the adjusting knob changes the flow rate of the liquid in the first pipe by squeezing the first pipe.
[0014] Furthermore, the delivery component also includes a sealing rubber plug, which is installed on the solid phase extraction column, and the first pipeline is penetrated by the sealing rubber plug.
[0015] Furthermore, the solid-liquid extraction device also includes a ball valve, and the ball valve is used to control the opening and closing of the first pipeline.
[0016] Furthermore, the negative pressure device includes a vacuum pump, and the vacuum pump is connected to the negative pressure connection end through a connecting pipe.
[0017] Furthermore, the solid phase extraction column is made of transparent material.
[0018] Furthermore, the extraction device body comprises a solid phase extractor, and the collection chamber is formed in the solid phase extractor.
[0019] Furthermore, it also includes a reagent bottle, and the other end of the first pipeline is arranged in the reagent bottle to transport the liquid in the reagent bottle to the solid phase extraction column.
[0020] According to the solid phase extraction automatic sampling equipment of the embodiment of the present application, at least the following beneficial effects are achieved: when performing extraction, the negative pressure device is activated to create a negative pressure environment for the collection chamber, the solution to be processed enters the solid phase extraction column through the first pipe, and the waste liquid enters the collection chamber through the liquid inlet connection end. The setting of the flow rate regulating device can adjust the flow rate of the liquid in the first pipe, thereby effectively controlling the solid phase extraction time of the solution to be processed and reducing the situation where there are obvious differences in the recovery rates of different samples caused by different flow rates. In addition, the embodiment of the present application can adjust the extraction flow rate and control the extraction time, which can effectively improve the probability of reproducibility of experimental results.
[0021] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] The present application is further described below with reference to the accompanying drawings and embodiments, wherein:
[0023] Figure 1 This is a schematic diagram of the structure of a solid phase extraction automatic sampling device according to an embodiment of the present application;
[0024] Figure 2 This is a schematic diagram of the structure of a delivery component in a solid phase extraction automatic sampling device according to an embodiment of the present application;
[0025] Figure 3 This is a schematic diagram of the structure of a flow rate regulating device in a solid phase extraction automatic sampling device according to an embodiment of the present application.
[0026] Reference numerals:
[0027] 110, extraction device body; 120, liquid inlet connection end; 130, negative pressure connection end;
[0028] 210, first pipeline; 220, solid phase extraction column; 230, flow rate regulating device; 231, matrix; 2311, channel; 232, adjusting knob; 240, sealing plug; 250, reagent bottle; 260, ball valve; 310, vacuum pump; 320, connecting pipeline. DETAILED DESCRIPTION
[0029] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.
[0030] In the description of the present application, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.
[0031] In the description of this application, "several" means more than one, "more" means more than two, "greater than", "less than", "exceed", etc. are understood to exclude the number itself, and "above", "below", "within", etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.
[0032] In the description of this application, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in this application based on the specific content of the technical solution.
[0033] In the description of the present application, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.
[0034] See also Figures 1 to 3 The embodiment of the present application discloses a solid phase extraction automatic sampling device, which includes a solid-liquid extraction device, a conveying component and a negative pressure device.
[0035] Specifically, the solid-liquid extraction device includes an extraction device body 110, a liquid inlet connection end 120 and a negative pressure connection end 130, the extraction device body 110 has a collection chamber, the liquid inlet connection end 120 is installed on the extraction device body 110 and is connected to the collection chamber, and the negative pressure connection end 130 is installed on the extraction device body 110 and is connected to the collection chamber; the conveying component includes a first pipeline 210, a solid phase extraction column 220 and a flow rate regulating device 230, the liquid inlet end of the solid phase extraction column 220 is connected to one end of the first pipeline 210, the flow rate regulating device 230 is used to adjust the flow rate of the fluid in the first pipeline 210, and the output end of the solid phase extraction column 220 is connected to the liquid inlet connection end 120; the negative pressure device is connected to the negative pressure connection end 130.
[0036] In the embodiment of the present application, when extraction is performed, the negative pressure device is activated to create a negative pressure environment for the collection chamber, and the solution to be treated is sucked into the solid phase extraction column 220 through the first pipe 210, and the waste liquid enters the collection chamber through the liquid inlet connection end 120. The flow rate regulating device 230 is set to adjust the flow rate of the liquid in the first pipe 210, so as to effectively control the solid phase extraction time of the solution to be treated, and reduce the situation where there is a significant difference in the recovery rate between different samples due to different flow rates.
[0037] In some embodiments of the present application, see Figure 1 As shown, there are multiple liquid inlet connection ends 120, and the multiple liquid inlet connection ends 120 are arranged at intervals on the extraction device body 110. Among them, each liquid inlet connection end 120 can be connected to a solid phase extraction column 220; and each solid phase extraction column 220 can be used to connect with different first pipes 210 to simultaneously process the same or different solutions to be processed. In this way, the ability of the solid phase extraction automatic sampling device to extract the solution to be processed can be improved, which is conducive to improving the extraction efficiency.
[0038] In practical applications, the number of the liquid inlet connection ends 120 and the solid phase extraction columns 220 can be set according to actual extraction needs to meet different extraction needs.
[0039] It should be noted that during extraction, the liquid inlet connection end 120 not connected to the solid phase extraction column 220 is sealed to ensure the vacuum degree in the collection chamber, thereby ensuring that the solution to be treated can smoothly enter the solid phase extraction column 220 from the first pipe 210.
[0040] In some embodiments of the present application, the flow rate regulating device 230 includes an infusion regulator or a flow regulating valve.
[0041] In a possible implementation, the flow rate regulating device 230 includes a flow regulating valve, which is installed on the first pipeline 210. In this way, the flow area in the first pipeline 210 can be changed by the flow regulating valve, thereby achieving the purpose of changing the flow rate of the liquid in the first pipeline 210.
[0042] In some embodiments of the present application, see Figure 2 and Figure 3 The infusion regulator includes a base 231 and an adjusting knob 232 . The base 231 is provided with a channel 2311 for accommodating the first pipe 210 . The adjusting knob 232 is mounted on the base 231 . The adjusting knob 232 changes the flow rate of the liquid in the first pipe 210 by squeezing the first pipe 210 .
[0043] In a possible implementation, the adjusting knob 232 is a circular roller, which is eccentrically disposed on the base 231, and one side of the circular roller abuts against the first pipe 210. By rotating the circular roller, the circular roller can be pressed against or away from the first pipe 210, thereby changing the flow rate of the liquid in the first pipe 210.
[0044] It is worth to understand that, in the above embodiment, the first pipe 210 is made of a soft material, so that it can be squeezed and deformed.
[0045] In some embodiments of the present application, see Figure 1 and Figure 2 The delivery assembly further includes a sealing rubber plug 240, which is installed on the solid phase extraction column 220, and the first pipe 210 is inserted into the sealing rubber plug 240. The first pipe 210 is also sealed and connected to the sealing rubber plug 240. In this way, the sealing performance between the first pipe 210 and the solid phase extraction column 220 can be improved, thereby ensuring that the solution to be treated can smoothly pass through the first pipe 210 into the solid phase extraction column 220 under negative pressure conditions.
[0046] In some embodiments of the present application, the extraction device body 110 includes a solid phase extractor, and a collection chamber is formed in the solid phase extractor.
[0047] In some embodiments of the present application, the solid-liquid extraction device further includes a ball valve 260 , and the ball valve 260 is used to control the on-off of the first pipeline 210 .
[0048] As one embodiment, the solid-liquid extraction device also includes a guide column, and the ball valve 260 includes a valve stem, a valve body and a valve core. There are circular holes on the valve core. The valve body is installed on the liquid inlet connection end 120 through the guide column, and the valve core is installed in the valve body. The valve stem is used to drive the valve core to move. When the direction of the circular hole is the same as the direction of the guide column, water can pass through the circular hole; rotating the valve stem causes the valve core to shift, so that the direction of the circular hole is different from the direction of the guide column, and the guide column is closed. In this way, the liquid inlet connection end 120 on the solid phase extractor can be sealed, thereby freely reducing or expanding the number of solid phase extraction samples.
[0049] In some embodiments of the present application, the negative pressure device includes a vacuum pump 310, which is connected to the negative pressure connection end 130 via a connecting pipe 320. By starting the vacuum pump 310, a vacuum environment can be created in the collection chamber, thereby driving the treated solution into the solid phase extraction column 220.
[0050] In some embodiments of the present application, the solid phase extraction column 220 is made of a transparent material, so that the staff can observe the situation inside the solid phase extraction column 220 conveniently.
[0051] In some embodiments of the present application, a reagent bottle 250 is further included, and the other end of the first pipe 210 is disposed in the reagent bottle 250 to transport the liquid in the reagent bottle 250 to the solid phase extraction column 220. The solution to be treated is placed in the reagent bottle 250. In practical applications, when the first pipe 210, the solid phase extraction column 220 and the collection chamber are connected, the vacuum pump 310 is started to allow the solution to be treated in the reagent bottle 250 to flow to the solid phase extraction column 220.
[0052] It is worth to understand that the reagent bottle 250 may also be other containers for storing solutions, which is not limited here.
[0053] Refer to the following Figures 1 to 3 The solid phase extraction automatic sampling device of the embodiment of the present application is described in detail with a specific embodiment. It should be understood that the following embodiment is only an exemplary description and should not be construed as limiting the embodiment of the present application.
[0054] See also Figures 1 to 3 The solid phase extraction automatic sampling device includes a large-capacity reagent bottle 250, a first pipeline 210, a flow rate regulating device 230, a solid phase extraction column, a sealing plug 240, a solid phase extraction instrument, a connecting pipeline 320 and a vacuum pump 310.
[0055] Specifically, the first pipe 210 is a hose, one end of which is a suction end placed in a large-capacity reagent bottle 250 , and the other end passes through a sealing rubber plug 240 and is placed in a solid phase extraction column 220 .
[0056] The first pipeline 210 is provided with a flow rate regulating device 230, which is an infusion regulator. If the sample solution flow rate is too fast, the organic compound will stay in the adsorbent for a short time, the compound adsorption rate will be low, and the recovery rate will be reduced. If the solution flow rate is too slow, the extraction time will increase. In the embodiment of the present application, by adding a flow rate regulating device to regulate the sample solution injection speed, it is beneficial to improve the solid phase extraction effect.
[0057] When performing solid phase extraction of large-volume water samples, the sample solution to be processed is loaded into the reagent bottle 250, and the suction end of the first pipe 210 is placed at the bottom of the reagent bottle 250. Under the negative pressure provided by the vacuum pump 310, the solution in the reagent bottle 250 enters the solid phase extraction column 220 through the first pipe 210. During the extraction process, the liquid flow rate is adjusted by the flow rate regulating device 230, and the organic compounds in the solution are adsorbed by the adsorbent in the solid phase extraction column 220. The waste liquid enters the solid phase extraction instrument through the liquid inlet connection end 120 for collection, and the subsequent solid phase extraction steps such as rinsing and elution can be completed by the solid phase extraction instrument.
[0058] This embodiment can realize the application of high-throughput separation and enrichment of organic matter in large-volume water samples by matching with a commercial multi-channel solid phase extractor, replacing the manual sample addition operation; the simple flow rate regulating device 230 and the closed solid phase extraction column 220 can adjust the extraction flow rate and control the extraction time, which is conducive to improving the reproducibility of experimental results, realizing large-volume water sample batch automatic addition, and effectively improving the water sample processing throughput and efficiency. Compared with the existing solid phase extraction sample introduction device, the solid phase extraction automatic sample introduction device in this application is simple in structure and operation, can effectively shorten the extraction time of the sample, improve the enrichment and separation efficiency, has a low manufacturing cost, and is convenient for promotion and use.
[0059] The embodiments of the present application are described in detail above in conjunction with the accompanying drawings, but the present application is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.
Claims
1. A solid phase extraction automatic sampling device, characterized in that: include: A solid-liquid extraction device, comprising an extraction device body, a liquid inlet connection end and a negative pressure connection end, wherein the extraction device body has a collection chamber, the liquid inlet connection end is mounted on the extraction device body and communicates with the collection chamber, and the negative pressure connection end is mounted on the extraction device body and communicates with the collection chamber; A delivery assembly, comprising a first pipeline, a solid phase extraction column and a flow rate regulating device, wherein the liquid inlet end of the solid phase extraction column is connected to one end of the first pipeline, the flow rate regulating device is used to regulate the flow rate of the fluid in the first pipeline, and the output end of the solid phase extraction column is connected to the liquid inlet connection end; A negative pressure device is connected to the negative pressure connection end.
2. The solid phase extraction automatic sampling device according to claim 1, characterized in that: There are multiple liquid inlet connection ends, and the multiple liquid inlet connection ends are arranged on the extraction device body at intervals.
3. The solid phase extraction automatic sampling device according to claim 1 or 2, characterized in that: The flow rate regulating device includes an infusion regulator or a flow regulating valve.
4. The solid phase extraction automatic sampling device according to claim 3, characterized in that: The infusion regulator includes a base body and an adjusting knob. The base body is provided with a channel for accommodating the first pipe. The adjusting knob is mounted on the base body. The adjusting knob changes the flow rate of the liquid in the first pipe by squeezing the first pipe.
5. The solid phase extraction automatic sampling device according to claim 1, characterized in that: The delivery assembly further comprises a sealing rubber plug, which is installed on the solid phase extraction column, and the first pipeline is passed through the sealing rubber plug.
6. The solid phase extraction automatic sampling device according to claim 1, characterized in that: The solid-liquid extraction device further comprises a ball valve, and the ball valve is used to control the opening and closing of the first pipeline.
7. The solid phase extraction automatic sampling device according to claim 1, characterized in that: The negative pressure device comprises a vacuum pump, and the vacuum pump is connected to the negative pressure connection end through a connecting pipe.
8. The solid phase extraction automatic sampling device according to claim 1, characterized in that: The solid phase extraction column is made of transparent material.
9. The solid phase extraction automatic sampling device according to claim 1, characterized in that: The extraction device body comprises a solid phase extractor, and the collection chamber is formed in the solid phase extractor.
10. The solid phase extraction automatic sampling device according to claim 1, characterized in that: It also includes a reagent bottle, in which the other end of the first pipeline is arranged to transport the liquid in the reagent bottle to the solid phase extraction column.