Culture medium constant-temperature cultivation device for preventing tissue culture browning of Dali tea

Abstract

The utility model relates to the field of constant-temperature culture medium cultivation devices, in particular to a constant-temperature culture medium cultivation device for preventing tissue culture browning of Dali tea. The technical problem that in the existing tissue culture process of Dali tea, browning phenomena occur on stems, leaves and embryos of explants, so that the Dali tea is delayed in growth or directly dies is solved. According to the technical scheme, the culture medium constant-temperature cultivation device for preventing tissue culture browning of Dali tea comprises a shell assembly, an adding assembly, a rotating assembly, a covering assembly and a cultivation assembly. According to the utility model, special anti-browning measures are adopted to prevent the browning phenomenon of the Dali tea in the tissue culture process, so that the problem of delayed growth or direct death of the Dali tea caused by the browning phenomenon on stem sections, leaves and embryos of explants in the existing tissue culture process of the Dali tea is solved, and the tissue culture quality of the Dali tea is improved. Therefore, the working efficiency of the whole culture medium constant-temperature cultivation device can be greatly improved.

Description

Technical Field

[0001] The utility model relates to the field of a culture medium constant temperature cultivation device, in particular to a culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning. Background Art

[0002] Dali tea (Camellia aliensis (WWSm.) Melch.) is a shrub or small tree of the Theaceae family and the genus Camellia. It is a general term for large-leaf tea varieties. As an important wild relative of tea, Dali tea is not only an important component of tea resources, but also participates in the domestication process of cultivated tea trees. The chemical composition of Dali tea is very similar to that of cultivated tea trees. Dali tea is rich in caffeine and tea polyphenols, and is a wild tea plant with a chemical composition that is relatively close to that of cultivated tea trees. However, the existing Dali tea often shows browning in some explants during tissue culture, and the explant types include stem segments, leaves and embryos. The appearance of browning directly affects the subsequent growth and development links such as subculture and differentiation of tissue culture seedlings, leading to the death of tissue culture seedlings, which can easily affect the tissue culture efficiency of the entire Dali tea. Utility Model Content

[0003] In order to overcome the problem that the existing Dali tea will undergo browning on the stem segments, leaves and embryos of the explants during tissue culture, thereby causing the Dali tea to experience growth delay or direct death.

[0004] The technical scheme of the utility model is: a culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning, comprising a shell component, an adding component, a rotating component, a covering component and a cultivation component; the adding component is installed on the upper end of the shell component; a rotating component is arranged on one side of the adding component; the covering component is arranged at the lower end of the adding component; and the cultivation component is arranged at the lower end of the covering component.

[0005] Preferably, the addition of tissue fluid is achieved by setting up an adding component, the rotation adjustment of the culture lamp is achieved by setting up a rotating component, the shielding treatment of Dali tea is achieved by setting up a covering component, and the cultivation treatment of Dali tea is achieved by setting up a cultivation component.

[0006] Preferably, the shell assembly includes a fixed shell, a support frame, a partition bar and a fixed plate; a plurality of support frames are installed on the outer side of the fixed shell; a partition bar is installed on the fixed shell; and a fixed plate is provided on one side of the partition bar.

[0007] Preferably, the adding component includes a mounting frame, an adding tube, a placement box and an electric push rod; a mounting frame is provided on the other side of the partition bar; an adding tube is installed on the mounting frame; a placement box is provided at the rear end of the adding tube; and an electric push rod is installed on one side of the placement box.

[0008] Preferably, the rotating assembly includes a driving motor, an active rotating wheel, a passive rotating wheel, a fixed turntable and an irradiation lamp; the driving motor is installed on the upper end of the fixed plate; the lower end of the driving motor is rotatably connected to the active rotating wheel via a rotating shaft; a passive rotating wheel is arranged at the front end of the active rotating wheel; the lower end of the passive rotating wheel is rotatably connected to the fixed turntable via a rotating shaft; and a plurality of irradiation lamps are installed on the fixed turntable.

[0009] Preferably, the covering assembly includes a rotating frame, a fixed through hole, a connecting strip, a buffer plate, a reset spring and a positioning rod; the inner side of the fixed shell is rotatably connected to the rotating frame via a rotating shaft; a fixed through hole is opened on the rotating frame; a connecting strip is fixedly connected to the inner wall of the fixed shell; a reset spring is arranged at the upper end of the connecting strip; a positioning rod is arranged on the inner side of the reset spring; and a buffer plate is arranged at the upper end of the positioning rod.

[0010] Preferably, the cultivation assembly includes a protective frame, a mobile box, an installation groove, a heating plate, a cultivation box, an electric slide, an electric slide rail, a supporting slide sleeve and a supporting slide rail; a protective frame is provided at the lower end of the rotating frame; a mobile box is provided at the lower end of the protective frame; a plurality of installation grooves are opened on the inner side of the mobile box; and a heating plate is provided on the inner side of the installation groove.

[0011] Preferably, a heating resistor wire is provided inside the heating plate; an incubation box is provided between two adjacent heating plates; an electric slide is installed at the lower end of the mobile box; the lower end of the electric slide is electrically connected to an electric slide rail; a supporting slide rail is provided at the front and rear ends of the electric slide rail; a supporting slide sleeve is provided at the upper end of the supporting slide rail.

[0012] Beneficial effects of the utility model:

[0013] 1. By setting up special anti-browning measures to prevent Dali tea from browning during tissue culture, the problem of browning on the stem segments, leaves and embryos of the explants of Dali tea during tissue culture, which leads to growth delay or direct death of Dali tea, can be solved, thereby greatly improving the working efficiency of the entire culture medium constant temperature cultivation device;

[0014] 2. The entire mobile box is driven to move by setting an electrically connected electric slide and an electric slide rail, so that the Dali tea inside the mobile box can be dark-cultured under the cover of the protective frame and the rotating frame, thereby reducing the browning phenomenon to a certain extent. At the same time, the mobile box is continuously driven by the electrically connected electric slide and the electric slide rail to switch between light and dark, so as to avoid the whitening phenomenon of the budding part, thereby greatly improving the cultivation life of the entire Dali tea. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The first three-dimensional structure schematic diagram of the culture medium constant temperature cultivation device of the utility model is shown;

[0016] Figure 2 The second three-dimensional structure schematic diagram of the culture medium constant temperature cultivation device of the utility model is shown;

[0017] Figure 3 The figure shows a three-dimensional structure diagram of the first covering component of the culture medium constant temperature cultivation device of the present utility model;

[0018] Figure 4 The figure shows a three-dimensional structure diagram of the second cover component of the culture medium constant temperature cultivation device of the present invention;

[0019] Figure 5 What is shown is a schematic diagram of the three-dimensional structure of the cultivation component of the culture medium constant temperature cultivation device of the present invention.

[0020] Explanation of the reference numerals: 1. Shell assembly; 2. Adding assembly; 3. Rotating assembly; 4. Covering assembly; 5. Cultivation assembly; 101. Fixed shell; 102. Support frame; 103. Spacer; 104. Fixed plate; 201. Mounting frame; 202. Adding tube; 203. Placement box; 204. Electric push rod; 301. Driving motor; 302. Active rotating wheel; 303. Passive rotating wheel; 304. Fixed turntable; 305. Irradiation lamp; 401. Rotating frame; 402. Fixed through hole; 403. Connecting strip; 404. Buffer sheet; 405. Return spring; 406. Positioning rod; 501. Protective frame; 502. Moving box; 503. Mounting groove; 504. Heating plate; 505. Cultivation box; 506. Electric slide; 507. Electric slide rail; 508. Support sleeve; 509. Support slide rail. DETAILED DESCRIPTION

[0021] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0022] See also Figure 1-2The utility model provides an embodiment: a culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning, comprising a shell component 1, an adding component 2, a rotating component 3, a covering component 4 and a cultivation component 5; the upper end of the shell component 1 is equipped with the adding component 2; the one side of the adding component 2 is provided with the rotating component 3; the lower end of the adding component 2 is provided with the covering component 4; the lower end of the covering component 4 is provided with the cultivation component 5, the shell component 1 comprises a fixed shell 101, a support frame 102, a partition bar 103 and a fixed plate 104; a plurality of support frames 102 are installed on the outer side of the fixed shell 101; the partition bar 103 is installed on the fixed shell 101; the one side of the partition bar 103 is provided with a fixed plate 104, the adding component 2 comprises a mounting frame 201, an adding tube 202, A placement box 203 and an electric push rod 204; a mounting frame 201 is provided on the other side of the partition bar 103; an addition tube 202 is installed on the mounting frame 201; a placement box 203 is provided at the rear end of the addition tube 202; an electric push rod 204 is installed on one side of the placement box 203, and the rotating component 3 includes a driving motor 301, an active rotating wheel 302, a passive rotating wheel 303, a fixed turntable 304 and an irradiation lamp 305; a driving motor 301 is installed at the upper end of the fixed plate 104; the lower end of the driving motor 301 is rotatably connected to the active rotating wheel 302 through a rotating shaft; a passive rotating wheel 303 is provided at the front end of the active rotating wheel 302; the lower end of the passive rotating wheel 303 is rotatably connected to the fixed turntable 304 through a rotating shaft; and a plurality of irradiation lamps 305 are installed on the fixed turntable 304.

[0023] See also Figure 3-4In this embodiment, the cover assembly 4 includes a rotating frame 401, a fixed through hole 402, a connecting strip 403, a buffer sheet 404, a reset spring 405 and a positioning rod 406; the inner side of the fixed shell 101 is rotatably connected with the rotating frame 401 through a rotating shaft; a fixed through hole 402 is opened on the rotating frame 401; a connecting strip 403 is fixedly connected to the inner wall of the fixed shell 101; a reset spring 405 is arranged at the upper end of the connecting strip 403; a positioning rod 406 is arranged at the inner side of the reset spring 405; a buffer sheet 404 is arranged at the upper end of the positioning rod 406, and the cultivation assembly 5 includes a protective frame 501, a moving box 502, a mounting groove 503, a heating plate 504, a cultivation box 505, an electric slide 506 , electric slide rail 507, supporting sleeve 508 and supporting slide rail 509; a protective frame 501 is arranged at the lower end of the rotating frame 401; a mobile box 502 is arranged at the lower end of the protective frame 501; a plurality of mounting grooves 503 are opened on the inner side of the mobile box 502; a heating plate 504 is arranged on the inner side of the mounting groove 503, and a heating resistance wire is arranged inside the heating plate 504; an incubator 505 is arranged between two adjacent heating plates 504; an electric slide rail 506 is installed at the lower end of the mobile box 502; the lower end of the electric slide rail 506 is electrically connected to the electric slide rail 507; a supporting slide rail 509 is arranged at the front and rear ends of the electric slide rail 507; a supporting sleeve 508 is arranged at the upper end of the supporting slide rail 509.

[0024] During operation, the Dali tea is placed inside the cultivation box 505, and the entire mobile box 502 is driven to move by the electrically connected electric slide 506 and the electric slide rail 507, so that the large-scale tea inside the mobile box 502 can be dark-cultured under the shielding of the protective frame 501 and the rotating frame 401, thereby reducing the browning phenomenon to a certain extent. At the same time, the mobile box 502 is continuously driven to switch between light and dark through the electrically connected electric slide 506 and the electric slide rail 507 to avoid the whitening phenomenon of the budding part, thereby realizing the use of the cultivation device to a certain extent.

[0025] Through the above steps, special anti-browning measures are set to prevent Dali tea from browning during the tissue culture process, so as to solve the problem that the existing Dali tea will have browning on the stem segments, leaves and embryos of the explants during the tissue culture process, thereby causing the growth delay or direct death of the Dali tea, thereby greatly improving the working efficiency of the entire culture medium constant temperature cultivation device. At the same time, the addition of tissue fluid can be achieved through the setting of the adding component 2, the rotation adjustment of the cultivation lamp can be achieved through the setting of the rotating component 3, the shielding treatment of the Dali tea can be achieved through the setting of the covering component 4, and the cultivation treatment of the Dali tea can be achieved through the setting of the cultivation component 5.

[0026] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the purpose of the present invention.

Claims

1. A culture medium constant temperature cultivation device for preventing Dali tea from browning during tissue culture, comprising a housing component (1); characterized in that: It also comprises an adding component (2), a rotating component (3), a covering component (4) and a cultivation component (5); the adding component (2) is installed at the upper end of the shell component (1); the rotating component (3) is arranged on one side of the adding component (2); and the covering component (4) is arranged at the lower end of the adding component (2); A cultivation component (5) is arranged at the lower end of the covering component (4).

2. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 1, characterized in that: The housing assembly (1) comprises a fixed shell (101), a support frame (102), a spacer (103) and a fixed plate (104); a plurality of support frames (102) are installed on the outside of the fixed shell (101); a spacer (103) is installed on the fixed shell (101); and a fixed plate (104) is provided on one side of the spacer (103).

3. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 2, characterized in that: The adding component (2) comprises a mounting frame (201), an adding pipe (202), a placement box (203) and an electric push rod (204); the mounting frame (201) is arranged on the other side of the partition bar (103); the adding pipe (202) is installed on the mounting frame (201); the placement box (203) is arranged at the rear end of the adding pipe (202); and the electric push rod (204) is installed on one side of the placement box (203).

4. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 2, characterized in that: The rotating assembly (3) comprises a driving motor (301), an active rotating wheel (302), a passive rotating wheel (303), a fixed rotating disk (304) and an irradiation lamp (305); the driving motor (301) is mounted on the upper end of the fixed plate (104); the lower end of the driving motor (301) is rotatably connected to the active rotating wheel (302) via a rotating shaft; the front end of the active rotating wheel (302) is provided with a passive rotating wheel (303); the lower end of the passive rotating wheel (303) is rotatably connected to the fixed rotating disk (304) via a rotating shaft; and a plurality of irradiation lamps (305) are mounted on the fixed rotating disk (304).

5. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 2, characterized in that: The cover assembly (4) comprises a rotating frame (401), a fixed through hole (402), a connecting strip (403), a buffer sheet (404), a reset spring (405) and a positioning rod (406); the inner side of the fixed housing (101) is rotatably connected to the rotating frame (401) via a rotating shaft; the rotating frame (401) is provided with a fixed through hole (402); the connecting strip (403) is fixedly connected to the inner wall of the fixed housing (101); the upper end of the connecting strip (403) is provided with a reset spring (405); the inner side of the reset spring (405) is provided with a positioning rod (406); A buffer sheet (404) is provided at the upper end of the positioning rod (406).

6. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 5, characterized in that: The cultivation component (5) comprises a protection frame (501), a movable box (502), a mounting groove (503), a heating plate (504), a cultivation box (505), an electric slide frame (506), an electric slide rail (507), a supporting slide sleeve (508) and a supporting slide rail (509); the lower end of the rotating frame (401) is provided with a protection frame (501); the lower end of the protection frame (501) is provided with a movable box (502); a plurality of mounting grooves (503) are provided on the inner side of the movable box (502); and a heating plate (504) is provided on the inner side of the mounting groove (503).

7. A culture medium constant temperature cultivation device for preventing Dali tea tissue culture from browning according to claim 6, characterized in that: A heating resistance wire is arranged inside the heating plate (504); an incubator (505) is arranged between two adjacent heating plates (504); an electric slide (506) is installed at the lower end of the moving box (502); the lower end of the electric slide (506) is electrically connected to an electric slide rail (507); a supporting slide rail (509) is arranged at the front and rear ends of the electric slide rail (507); and a supporting slide sleeve (508) is arranged at the upper end of the supporting slide rail (509).

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