Microplate of microplate reader

Abstract

The utility model provides a microplate reader microplate, which relates to the microplate reader microplate field, and comprises a plate body and a cover plate, the plate body is internally provided with a grid plate, the grid plate is internally provided with a placing groove, the placing groove is internally provided with a containing assembly, the containing assembly comprises utensils, connecting sheets and communicating pipes are connected between the utensils, and the connecting sheets and the communicating pipes are connected with the cover plate. Meanwhile, a handle is connected to the outer end of the vessel, guide columns are arranged at the four corners of one side of the cover plate, sealing blocks are arranged on the cover plate and on the end face of the same side with the guide columns, and meanwhile the guide columns are correspondingly connected with guide holes formed in the four corners of the upper end of the plate body in an inserted mode. The problems that samples need to be sequentially added into an existing microwell plate, it is inconvenient to guarantee that the doses of the samples in all the holes are the same, and meanwhile scattering occurs in the moving process are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of microplates for ELISA instruments, in particular to a microplate for ELISA instruments. Background Art

[0002] The microplate reader is a commonly used microplate reader. The microplate is used to hold the sample to be tested, and the microplate holder is used to fix the microplate. The driving mechanism in the microplate reader can drive the microplate holder to move in the microplate reader, so that the microplate moves to the preset position in the microplate reader. After searching, the prior art (application number: CN202220680486.8) records "a microplate holder and a microplate reader, the microplate holder is used for a microplate reader with a calibration mechanism, and belongs to the field of biomedical testing technology. The microplate holder includes a holder and a fastening mechanism, the holder is provided with a placement slot, the placement slot is used to hold the microplate, the fastening mechanism is installed on the holder, the fastening mechanism is used to fasten the microplate in the placement slot, wherein the holder is provided with a calibration part, the calibration part is used for detection and identification by the calibration mechanism, so that the microplate is located at the preset position of the microplate reader. The calibration part provided on the holder of the microplate holder can be detected by the calibration mechanism of the microplate reader to determine whether the microplate is located at the preset position in the microplate reader, thereby improving the accuracy of the detection results of the microplate reader."

[0003] However, although the microplate of the ELISA reader in the prior art has achieved precise positioning through the bracket, it still has some shortcomings: the existing microplate of the ELISA reader is generally a simple rectangular plate structure with a container inside, and when adding samples and reagents, it is necessary to ensure that the sample amount in each well of the microplate is strictly consistent, which puts higher requirements on the operating skills of the experimenter, but the existing microplate needs to be added one by one, and the process is more cumbersome, and microplates with a large number of wells (such as 96-well microplates) may be missed; secondly, the microplate needs to be moved or transferred a short distance after the sample is added during operation, and the existing microplate lacks an anti-leakage structure, which means that shaking during movement may cause sample leakage, which is inconvenient to use. Utility Model Content

[0004] In order to overcome the defects of the prior art, a microplate for an ELISA reader is provided to solve the problem that the existing microplate needs to add samples sequentially, is not convenient for ensuring the same sample dosage in each well, and has the problem of spillage during movement.

[0005] To achieve the above-mentioned purpose, a microplate for an ELISA instrument is provided, comprising: a plate body and a cover plate, wherein a square grid plate is arranged inside the plate body, and a placement groove is arranged in the square grid plate, and a holding component is placed in the placement groove, wherein the holding component comprises a vessel, and a connecting piece and a connecting pipe are connected between the vessels, and a handle is connected to the outer end of the vessel, guide columns are arranged at the four corners of one side of the cover plate, and a sealing block is arranged on the cover plate and on the same side end face as the guide column, and the guide column is correspondingly plugged into the guide holes opened at the four corners of the upper end of the plate body.

[0006] Furthermore, the plate body is configured as a rectangular plate structure, and a notch is provided on one side edge of the rectangular plate, and the notch is correspondingly plugged into the handle, and the handle protrudes outward from the notch.

[0007] Furthermore, two corner ends on one side of the plate body are provided with guide ends, and the guide ends are configured as triangular blocks protruding outwards, and the guide ends are located on one side of the notch groove.

[0008] Furthermore, the lattice plates are configured as horizontal and vertical plates of a cross structure, and a clearance groove is provided at the upper end of the horizontal plate, and the connecting pipe and the connecting piece are clamped in the clearance groove.

[0009] Furthermore, the vessel is configured as a cylindrical structure made of quartz material, and the bottom of the vessel is configured as a blocking end, while the connecting pipe is connected to a position of the vessel near the bottom.

[0010] Furthermore, the cover plate is configured as a rectangular plate structure, and a lifting rod with an N-shaped structure is provided on the end surface of the cover plate on the side opposite to the sealing block.

[0011] Furthermore, the sealing block is configured as a conical structure made of polytetrafluoroethylene, and the outer end of the sealing block is configured as a narrow end of the conical structure, and the narrow end is matched and plugged with an open opening on the vessel.

[0012] The beneficial effects of the utility model are:

[0013] 1. When in use, place the holding assembly into the square plate inside the plate body, so that the handle is placed in the notch groove, the connecting pipe and the connecting piece are located in the give way groove opened at the upper end of the square plate, and the vessel is placed in the placement groove. Then, the adding device is used to add samples to any vessel in the same holding assembly. During the process, the vessels are connected to form a connecting vessel structure through the connecting pipe, thereby ensuring that the sample dosage in each vessel is the same when the addition is stopped, and there is no need to add the vessels one by one, thereby improving the operation efficiency;

[0014] 2. When moving the microplate to which samples have been added, first insert the four guide posts of the cover into the guide holes accordingly. At this time, insert the narrow end of the sealing block into the vessel, and then move the plate as a whole. Since the sealing block made of polytetrafluoroethylene is hydrophobic, the sealing block will not bring out the sample when the cover is removed (at the same time, the amount of sample added is generally small, and the sealing block will contact it after the cover is installed), thereby ensuring that the sample is not shaken and leaked during movement. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the plate structure of an embodiment of the utility model.

[0016] Figure 2 It is a schematic diagram of the sealing surface structure of the cover plate of an embodiment of the utility model.

[0017] Figure 3 It is a schematic diagram of the front structure of the containing assembly of an embodiment of the utility model.

[0018] Figure 4 It is a schematic diagram of the reverse structure of the containing component of an embodiment of the utility model.

[0019] In the figure: 1, plate body; 11, grid plate; 12, guide hole; 13, holding component; 131, handle; 132, container; 133, connecting piece; 134, connecting pipe; 14, notch groove; 15, guide end; 16, placement groove; 2, cover plate; 21, guide column; 22, sealing block. DETAILED DESCRIPTION

[0020] Reference Figures 1 to 4 As shown, the utility model provides an ELISA microplate, including: a plate body 1 and a cover plate 2, a grid plate 11 is provided inside the plate body 1, and a placement groove 16 is provided in the grid plate 11, and a holding component 13 is placed in the placement groove 16, and the holding component 13 includes a vessel 132, and a connecting piece 133 and a connecting pipe 134 are connected between the vessels 132, and a handle 131 is connected to the outer end of the vessel 132, and guide columns 21 are provided at four corners on one side of the cover plate 2, and a sealing block 22 is provided on the cover plate 2 and on the same side end face as the guide column 21, and the guide column 21 is correspondingly plugged into the guide holes 12 opened at the four corners of the upper end of the plate body 1.

[0021] In this embodiment, the plate body 1 and the cover plate 2 constitute the main structure of the microplate of the ELISA instrument involved in this application, and the microplate involved in this application has ninety-six wells.

[0022] The containing components 13 are configured into twelve groups in total, and each group includes eight containers 132 .

[0023] It should be noted that the attached Figure 1Not all of the containing components 13 inside the plate body 1 are drawn, only one group located at the left edge is drawn, and the structures of the other groups are the same.

[0024] As a preferred embodiment, the handle 131 is provided to facilitate taking out the entire containing assembly 13 for cleaning or replacement.

[0025] It should be noted that the adding device used during adding is kept in a full-dose state during the use of a containing component 13, for example, a syringe is used to ensure that the syringe is full of samples before adding. When the samples in the eight containers 132 exceed the position of the connecting tube 134, the scale line of the syringe is observed and recorded. When adding to the next group of containing components 13, the syringe is filled again, and then addition is performed based on the scale line recorded for the first time.

[0026] As a preferred implementation, the sample addition efficiency is improved by adding samples in groups, while the error of the reagent amount in the vessel 132 is greatly reduced.

[0027] like Figure 1 As shown, the plate body 1 is configured as a rectangular plate structure, and a notch groove 14 is provided on one edge of the rectangular plate, and the notch groove 14 is correspondingly plugged into the handle 131, and the handle 131 protrudes outward from the notch groove 14, and two corner ends on one side of the plate body 1 are provided with guide ends 15, and the guide ends 15 are configured as triangular blocks protruding outward, and the guide ends 15 are located on one side of the notch groove 14.

[0028] As a preferred embodiment, the notch groove 14 is provided to facilitate the positioning of the handle 131 and to facilitate taking out the containing assembly 13 through the handle 131 .

[0029] As a preferred embodiment, the guide end 15 is provided to ensure directionality when the microplate is placed in the microplate reader.

[0030] like Figure 1 , Figure 3 and Figure 4 As shown, the lattice plate 11 is configured as a horizontal and vertical plate of a cross structure, and a clearance groove is opened at the upper end of the horizontal plate (marked in the text, the clearance groove and the notch groove 14 are correspondingly located on the same straight line), and the connecting tube 134 and the connecting piece 133 are clamped in the clearance groove, the vessel 132 is configured as a cylindrical structure of quartz material, and the bottom of the vessel 132 is configured as a blocking end, and the connecting tube 134 is connected to a position near the bottom of the vessel 132.

[0031] As a preferred embodiment, the connection piece 133 is provided to facilitate connecting a plurality of containers 132 into a strip structure. Meanwhile, the connecting tube 134 not only realizes the connecting vessel principle, but also enhances the connection stability between the containers 132 .

[0032] As a preferred embodiment, by connecting the connecting tube 134 to a position near the bottom of the vessel 132, the sample can flow out to each vessel 132 in a timely manner during the adding process.

[0033] like Figure 2 As shown, the cover plate 2 is configured as a rectangular plate structure, and a lifting rod of an N-shaped structure (not marked in the text) is provided on the end face of the cover plate 2 on the opposite side to the sealing block 22. The sealing block 22 is configured as a conical structure of polytetrafluoroethylene material, and the outer end of the sealing block 22 is configured as a narrow end of the conical structure, and the narrow end is matched and plugged with the open mouth on the vessel 132.

[0034] Specifically, the wide end of the sealing block 22 is connected to the cover plate 2, and the outer diameter of the wide end is larger than the port of the container 132, thereby achieving a sealing effect.

[0035] When in use, the holding component is placed in the grid plate inside the plate body, so that the handle is placed in the notch groove, the connecting pipe and the connecting piece are located in the give way groove opened at the upper end of the grid plate, and the vessel is placed in the placement groove. Then, the sample is added to any vessel in the same holding component through the adding device. During the process, the vessels are connected to form a connecting vessel structure through the connecting pipe, thereby ensuring that the sample dosage in each vessel is the same when the addition is stopped; when moving the microplate to which the sample has been added, firstly, the four guide posts of the cover plate are correspondingly plugged into the guide holes, at this time, the narrow end of the sealing block is inserted into the vessel, and then the plate body is moved as a whole. Since the sealing block made of polytetrafluoroethylene is hydrophobic, the sealing block will not bring out the sample when the cover plate is removed.

[0036] The microplate for an ELISA reader of the utility model can effectively solve the problems that the existing microplate needs to add samples sequentially, is not convenient for ensuring that the sample dosage in each well is the same, and there is also the problem of spillage during movement. It improves the efficiency of adding samples on the basis of the existing microplate technology for an ELISA reader, makes it easy to ensure that the sample addition amount in each well is consistent, and facilitates quick disassembly of the vessel for cleaning or replacement, and prevents spillage during movement of the microplate.

Claims

1. A microplate reader, comprising: A plate body (1) and a cover plate (2), characterized in that: a lattice plate (11) is provided inside the plate body (1), and a placement groove (16) is provided in the lattice plate (11), and a holding component (13) is placed in the placement groove (16), and the holding component (13) includes a container (132), and a connecting piece (133) and a connecting pipe (134) are connected between the containers (132), and a handle (131) is connected to the outer end of the container (132), and guide columns (21) are provided at four corners on one side of the cover plate (2), and a sealing block (22) is provided on the cover plate (2) and on the same side end surface as the guide column (21), and the guide column (21) is correspondingly plugged into the guide holes (12) opened at the four corners of the upper end of the plate body (1).

2. The microplate for an ELISA reader according to claim 1, characterized in that: The plate body (1) is configured as a rectangular plate structure, and a notch groove (14) is provided on one side edge of the rectangular plate, and the notch groove (14) is correspondingly plugged into the handle (131), while the handle (131) protrudes outward from the notch groove (14).

3. The microplate for an ELISA reader according to claim 1, characterized in that: Two corner ends on one side of the plate body (1) are provided with guide ends (15), and the guide ends (15) are configured as triangular blocks protruding outwards, and the guide ends (15) are located on one side of the notch groove (14).

4. The microplate for an ELISA reader according to claim 1, characterized in that: The lattice plate (11) is configured as a cross-structured horizontal and vertical plate, and a clearance groove is provided at the upper end of the horizontal plate, and the connecting pipe (134) and the connecting piece (133) are clamped in the clearance groove.

5. The microplate for an ELISA reader according to claim 1, characterized in that: The vessel (132) is a cylindrical structure made of quartz material, and the bottom of the vessel (132) is a blocked end, while the connecting pipe (134) is connected to a position near the bottom of the vessel (132).

6. The microplate for an ELISA reader according to claim 1, characterized in that: The cover plate (2) is configured as a rectangular plate structure, and a lifting rod with an N-shaped structure is provided on the end surface of the cover plate (2) on the side opposite to the sealing block (22).

7. The microplate for an ELISA reader according to claim 1, characterized in that: The sealing block (22) is a conical structure made of polytetrafluoroethylene, and the outer end of the sealing block (22) is a narrow end of the conical structure, and the narrow end is matched and plugged with an open opening on the container (132).

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