Mechanical pressurization type chromatographic column packing device

Abstract

The utility model discloses a mechanical pressurization type chromatographic column packing device, which relates to the technical field of chromatographic columns and comprises a push rod component, a homogenate tank component, a chromatographic column component and a support seat component. According to the utility model, the first plug is used for opening and closing the exhaust and liquid outlet of the push rod assembly; the first connecting pipe is used for providing a passage for exhausting gas and liquid; the push rod head serves as a part in the homogenate tank assembly and in direct contact with homogenate, and the second connecting pipe provides an exhaust and liquid passage; the second plug is used for opening and closing an exhaust and liquid outlet of the chromatographic column assembly; the chromatographic column is packed in a mechanical pressurization mode, meanwhile, gas and liquid in slurry are rapidly discharged in the packing process, the packing period can be effectively shortened, meanwhile, after packing in the chromatographic column is pressurized for multiple times, the distribution uniformity and compactness of the packing can be effectively guaranteed, and the packing quality is improved. And the filling quality of the chromatographic column can be effectively ensured through a standardized operation process.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatographic columns, in particular to a mechanical pressurized chromatographic column packing device. Background Art

[0002] Chromatographic columns can be divided into two categories: packed columns and open tubular columns. Liquid chromatography usually uses packed columns. The separation effect of a chromatographic column depends mainly on the selected stationary phase and the preparation and operating conditions of the chromatographic column.

[0003] Patent (CN220455240U) discloses a column packing machine for a chromatograph, including a housing, a first limiting groove is provided inside the bottom end of the housing, a first spring is fixedly connected inside the first limiting groove, a rubber block is fixedly connected to the top wall of the first spring, a second limiting groove is provided inside the top end of the rubber block, a card plate is rotatably connected to the middle of the two connecting blocks, a placement groove is provided on the adjacent side of the two card plates, a slide groove is provided inside the card plate, a limiting column is fixedly connected inside the slide groove, a second spring is slidably connected inside the limiting column, a limiting plate is fixedly connected to the right side of the limiting column, and a plurality of rubber strips are fixedly connected to one side of the limiting plate. In the utility model, the chromatographic column is fixed when being filled and different types of chromatographic columns can be fixed to improve the stability of the device as a whole when in use.

[0004] The chromatographic column packing machine for chromatograph in the above patent document only packs the chromatographic column through a feeder, a quantitative valve and a receiving tube, and mainly relies on the dead weight of the filler to pack the column. The packing cycle is long, and the filling distribution uniformity and compactness of the filler are difficult to guarantee, resulting in unstable filling quality of the chromatographic column. Utility Model Content

[0005] The utility model aims to provide a mechanical pressurized chromatography column packing device to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the utility model provides the following technical solutions: a mechanical pressurized chromatographic column packing device, comprising a push rod assembly, a homogenization tank assembly, a chromatographic column assembly and a support seat assembly arranged in sequence from top to bottom, the chromatographic column assembly is arranged on the inner side of the support seat assembly, the push rod assembly is slidably sleeved on the inner side of the homogenization tank assembly, the push rod assembly comprises a first plug, a first connecting pipe, a push rod, a push rod head, and a filter screen plate, the push rod head is arranged at the bottom of the push rod, the first plug is connected to the push rod head through a first connecting pipe, the filter screen plate is arranged at the bottom of the push rod head, the push rod head is a hollow structure, the chromatographic column assembly comprises a chromatographic column, a second connecting pipe and a second plug, and the second plug is connected to the bottom of the chromatographic column through the second connecting pipe.

[0007] Furthermore, the first plug and the first connecting tube are fixedly connected via the first connecting tube, the first connecting tube is fixedly connected to the top of the push rod head via the second connecting tube, and the second plug is fixedly connected to the second connecting tube via the third connecting tube.

[0008] Furthermore, the homogenization tank assembly includes a tank body, a first sealing gasket and a connector, and the outer wall of the push rod head is provided with a plurality of sealing rings matching the tank body.

[0009] Furthermore, a first socket matching the tank body is provided at the top of the connector, and a second socket matching the chromatographic column is provided at the bottom of the connector. The first socket is connected to the second socket, and two first sealing gaskets are provided, which are respectively arranged on the inner sides of the first socket and the second socket.

[0010] Furthermore, the support seat assembly includes a support seat tube body and a support base, the support seat tube body is sleeved on the top of the support base, and the top of the support seat tube body is provided with an opening matching the connector.

[0011] Furthermore, the chromatographic column assembly also includes a support block, a second sealing gasket matching the chromatographic column is provided on the top of the support block, the second connecting pipe passes through the support block and is connected to the bottom of the chromatographic column, and a socket matching the support block is opened on the top of the support base.

[0012] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0013] 1. The utility model is provided with a push rod assembly, a homogenizing tank assembly, a chromatographic column assembly and a support seat assembly. The first plug is used to open and close the exhaust and liquid ports of the push rod assembly; the first connecting pipe is used to provide a passage for exhaust and liquid; the push rod is used as a force-bearing component in the compression process of the universal testing machine; the push rod head is used as a component that directly contacts the homogenizing liquid inside the homogenizing tank assembly; the sealing ring seals the push rod head and the tube wall of the tank body to ensure that the liquid does not leak; the filter screen plate is used for filtering to ensure that the solid particles in the homogenizing liquid do not leak; the tank body is used as a homogenizing liquid container, and the filler is fed into the chromatographic column through the tank body; the first sealing gasket is used to ensure that the tank body and the connector do not leak; the connector is used to connect the tank body and the chromatographic column; the chromatographic column is used to receive the filler; the second connecting pipe provides exhaust and liquid passages; the second plug is used to open and close the exhaust and liquid ports of the chromatographic column assembly.

[0014] 2. In the utility model, the present application performs column packing operation on the chromatographic column by mechanical pressurization, and quickly discharges the gas and liquid in the slurry during the column packing process, which can effectively shorten the column packing cycle. At the same time, after multiple pressurization treatments, the internal filler of the chromatographic column can effectively ensure the distribution uniformity and compactness of the filler, and the filling quality of the chromatographic column can be effectively guaranteed through standardized operation procedures. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0016] Figure 1 It is the front view of the utility model as a whole;

[0017] Figure 2 It is a front cross-sectional view of the utility model as a whole;

[0018] Figure 3 It is a front cross-sectional view of the push rod assembly of the utility model;

[0019] Figure 4 It is a front cross-sectional view of the homogenizing tank assembly of the utility model;

[0020] Figure 5 It is a front cross-sectional view of the chromatographic column assembly of the utility model;

[0021] Figure 6 It is a front cross-sectional view of the support seat assembly of the utility model;

[0022] In the figure: 1. push rod assembly; 11. first plug; 12. first connector; 13. first connecting tube; 14. push rod; 15. second connector; 16. push rod head; 17. sealing ring; 18. filter screen plate; 2. homogenization tank assembly; 21. tank body; 22. first sealing gasket; 23. connector; 3. chromatographic column assembly; 31. chromatographic column; 32. support block; 33. second connecting tube; 34. third connector; 35. second plug; 4. support seat assembly; 41. support seat tube body; 42. support base. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-Figure 6The utility model provides a technical solution: a mechanical pressurized chromatographic column packing device, comprising a push rod assembly 1, a homogenization tank assembly 2, a chromatographic column assembly 3 and a support seat assembly 4 which are arranged in sequence from top to bottom, the chromatographic column assembly 3 is arranged on the inner side of the support seat assembly 4, the push rod assembly 1 is slidably sleeved on the inner side of the homogenization tank assembly 2, the push rod assembly 1 comprises a first plug 11, a first connecting pipe 13, a push rod 14, a push rod head 16, and a filter screen plate 18, the push rod head 16 is arranged at the bottom of the push rod 14, the first plug 11 is connected to the push rod head 16 through the first connecting pipe 13, the filter screen plate 18 is arranged at the bottom of the push rod head 16, the push rod head 16 is a hollow structure, the chromatographic column assembly 3 comprises a chromatographic column 31, a second connecting pipe 33 and a second plug 35, the second plug 35 is connected to the bottom of the chromatographic column 31 through the second connecting pipe 33.

[0025] The first plug 11 and the first connecting tube 13 are fixedly connected via the first connecting head 12, the first connecting tube 13 is fixedly connected to the top of the push rod head 16 via the second connecting head 15, and the second plug 35 is fixedly connected to the second connecting tube 33 via the third connecting head 34; the homogenization tank assembly 2 comprises a tank body 21, a first sealing gasket 22 and a connector 23, the outer wall of the push rod head 16 is provided with a plurality of sealing rings 17 matching the tank body 21; the top of the connector 23 is provided with a first socket matching the tank body 21, the bottom of the connector 23 is provided with a second socket matching the chromatographic column 31, the first socket is connected to the second The two sockets are through-connected, and two first sealing gaskets 22 are provided, and the two first sealing gaskets 22 are respectively arranged on the inner sides of the first socket and the second socket; the support seat assembly 4 includes a support seat tube body 41 and a support base 42, and the support seat tube body 41 is sleeved on the top of the support base 42, and the top of the support seat tube body 41 is provided with an opening matching the connector 23; the chromatographic column assembly 3 also includes a support block 32, and the top of the support block 32 is provided with a second sealing gasket matching the chromatographic column 31, and the second connecting pipe 33 passes through the support block 32 and is connected with the bottom of the chromatographic column 31, and the top of the support base 42 is provided with a socket matching the support block 32.

[0026] The working principle of this utility model:

[0027] Refer to the instruction manual Figure 1-Figure 6The utility model is provided with a push rod assembly 1, a homogenizing tank assembly 2, a chromatographic column assembly 3 and a support seat assembly 4. The first plug 11 is used to open and close the exhaust and liquid ports of the push rod assembly 1; the first connecting pipe 13 is used to provide a passage for exhaust and liquid; the push rod 14 is used as a force-bearing component in the compression process of the universal testing machine; the push rod head 16 is a component that directly contacts the homogenizing liquid inside the homogenizing tank assembly 2; the sealing ring 17 seals the push rod head 16 and the tube wall of the tank body 21 to ensure that the liquid does not leak; the filter sieve plate 18 is used for filtering to ensure that the solid particles in the homogenizing liquid do not leak; the tank body 21 is used as a homogenizing liquid container, and the filler is fed into the chromatographic column 31 through the tank body 21; the first sealing gasket 22 is used to ensure that the tank body 21 and the connector 23 do not leak; the connector 23 is used to connect the tank body 21 and the chromatographic column 31; the chromatographic column 31 is used to receive the filler; the second connecting pipe 33 provides an exhaust and liquid passage; the second plug 35 is used to open and close the exhaust and liquid ports of the chromatographic column assembly 3;

[0028] Column packing process:

[0029] 1: Homogenization (for example, the filler (SiO2) and the homogenizing liquid (isopropanol) are mixed evenly in a ratio of 1:1 to 1:10, and ultrasonic degassing is performed);

[0030] 2: Fix the chromatographic column assembly 3 on the connector 23 at the bottom of the homogenizing tank assembly 2, place the chromatographic column assembly 3 at the bottom of the homogenizing tank assembly 2 in the support seat assembly 4, cover the connector 23 on the inner side of the opening at the top of the support seat tube 41, and place the assembled assembly on the universal testing machine;

[0031] 3: Pour the homogenized slurry into the tank body 21 of the homogenization tank assembly 2;

[0032] 4: Insert the push rod head 16 at the bottom of the push rod 14 into the tank body 21 of the homogenization tank assembly 2, and the sealing ring 17 is sealed between the push rod head 16 and the inner wall of the tank body 21, and the first plug 11 is opened. The universal testing machine slowly presses down the push rod 14 in the push rod assembly 1 (0.5-20mm / min), and the push rod 14 drives the push rod head 16 to press down. The push rod head 16 presses the air inside the tank body 21 downward, and the air inside the tank body 21 is pressed downward by the descending slurry used for squeezing. At the same time, the air inside the tank body 21 passes through the filter screen plate 18, and then passes through the push rod head 16 and the first connecting pipe 13, and then is discharged from the first plug 11 until no gas is discharged from the first connecting pipe 13, and the universal test is suspended, and the first plug 11 is closed;

[0033] 5: Open the second plug 35, adjust the pressing speed of the universal testing machine (5-100mm / min), start the universal testing machine, press the push rod 14 in the push rod assembly 1, and the push rod 14 pushes the slurry inside the tank 21 downward. After the slurry enters the chromatographic column 31, under the pressure of the push rod 14, the gas and liquid in the slurry inside the chromatographic column 31 are discharged from the second plug 35 through the second connecting pipe 33, which can effectively pressurize the filler inside the chromatographic column 31, so that the filler inside the chromatographic column 31 is more evenly and tightly distributed. When the pressure reaches the set pressure (3-20MPa), stop adjusting the universal testing machine;

[0034] 6: Close the second plug 35, open the first plug 11, adjust the pressing speed of the universal testing machine (1-5mm / min), start the universal testing machine, press down the push rod 14 in the push rod assembly 1, the push rod 14 continues to press the slurry inside the tank body 21 downward, and further pressurize the filler inside the chromatographic column 31, so that the filler inside the chromatographic column 31 is more evenly and tightly distributed. When the pressure reaches the set pressure (3-20MPa), stop adjusting the universal testing machine; maintain the pressure for 3-240 minutes;

[0035] 7: Remove the chromatographic column assembly 3 and fix the upper end cover of the chromatographic column 31. At this time, the column loading operation of the chromatographic column is completed;

[0036] The present application performs column packing operation on the chromatographic column 31 by mechanical pressurization, and quickly discharges the gas and liquid in the slurry during the column packing process, which can effectively shorten the column packing cycle. At the same time, after the internal filler of the chromatographic column 31 has been subjected to multiple pressurization treatments, the distribution uniformity and compactness of the filler can be effectively guaranteed, and the filling quality of the chromatographic column 31 can be effectively guaranteed through standardized operating procedures.

[0037] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A mechanical pressurized chromatographic column packing device, comprising a push rod assembly (1), a homogenizing tank assembly (2), a chromatographic column assembly (3) and a support assembly (4) arranged in sequence from top to bottom, characterized in that: The chromatographic column assembly (3) is arranged on the inner side of the support seat assembly (4); the push rod assembly (1) is slidably sleeved on the inner side of the homogenization tank assembly (2); the push rod assembly (1) comprises a first plug (11), a first connecting pipe (13), a push rod (14), a push rod head (16), and a filter screen plate (18); the push rod head (16) is arranged at the bottom of the push rod (14); the first plug (11) is connected to the push rod head (16) through the first connecting pipe (13); the filter screen plate (18) is arranged at the bottom of the push rod head (16); the push rod head (16) is a hollow structure; the chromatographic column assembly (3) comprises a chromatographic column (31), a second connecting pipe (33) and a second plug (35); the second plug (35) is connected to the bottom of the chromatographic column (31) through the second connecting pipe (33).

2. The mechanical pressurized chromatographic column packing device according to claim 1, characterized in that: The first plug (11) and the first connecting tube (13) are fixedly connected via a first connecting tube (12); the first connecting tube (13) is fixedly connected to the top of the push rod head (16) via a second connecting tube (15); and the second plug (35) is fixedly connected to the second connecting tube (33) via a third connecting tube (34).

3. The mechanical pressurized chromatographic column packing device according to claim 1, characterized in that: The homogenizing tank assembly (2) comprises a tank body (21), a first sealing gasket (22) and a connector (23), and the outer wall of the push rod head (16) is provided with a plurality of sealing rings (17) matching the tank body (21).

4. The mechanical pressurized chromatographic column packing device according to claim 3, characterized in that: The top of the connector (23) is provided with a first socket matching the tank body (21), and the bottom of the connector (23) is provided with a second socket matching the chromatographic column (31), the first socket is connected to the second socket, and two first sealing gaskets (22) are provided, and the two first sealing gaskets (22) are respectively arranged on the inner sides of the first socket and the second socket.

5. The mechanical pressurized chromatographic column packing device according to claim 3, characterized in that: The support seat assembly (4) comprises a support seat tube body (41) and a support base (42); the support seat tube body (41) is sleeved on the top of the support base (42); and an opening matching the connector (23) is provided on the top of the support seat tube body (41).

6. A mechanical pressurized chromatographic column packing device according to claim 5, characterized in that: The chromatographic column assembly (3) further comprises a support block (32), a second sealing gasket matching the chromatographic column (31) being provided on the top of the support block (32), the second connecting tube (33) passing through the support block (32) and being connected to the bottom of the chromatographic column (31), and a socket matching the support block (32) being provided on the top of the support base (42).

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