Strain slant preservation pipe and cooling support thereof

Abstract

The slant strain preservation tube and the cooling support thereof are characterized in that the preservation tube comprises a tube body and a tube cover, one side of the inner bottom surface and the inner tube wall of the tube body is a frosted ring surface, the other side of the inner bottom surface and the inner tube wall of the tube body is a transparent light tube, and a crescent blocking piece is arranged on the upper portion of the frosted surface of the inner tube wall; the tube cover comprises a hollow cylindrical cover body and a tube plug, and a semi-permeable membrane is arranged in a hollow cavity of the cover body. The cooling support comprises a cooling panel, a hinged shaft, a base and an adjusting rod. The panel is obliquely arranged, and the angle of the panel can be adjusted to obtain the optimal slope area. The culture tube has the advantages that the inner wall of the tube body part is frosted, so that the condition that a slant solid culture medium slides or falls off in the inoculation or strain taking process can be avoided. And the semi-permeable membrane is arranged in the tube cover, so that the strains can be effectively propagated on the inclined plane after being inoculated. And the blocking sheet arranged in the tube body can effectively prevent the condition that the internal culture medium is easy to overflow when the inclined plane is manufactured. According to the utility model, the survival rate and the preservation time of fungi or oomycetes strains in the low-temperature inclined plane preservation can be improved and prolonged.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganism preservation devices, in particular to a bacteria seed inclined surface preservation tube and a cooling bracket thereof. Background Art

[0002] In microbial research, strains need to be backed up and preserved. Commonly used strain preservation methods are bevel tube cryopreservation and glycerol ultra-low temperature freezing.

[0003] At present, most of the slant preservation tubes for strains on the market are glass test tube slant. This type of slant tube generally consists of a test tube containing a solid culture medium slant and a plug or lid. It has good sealing performance and is widely used in the cultivation and preservation of bacteria, but has certain limitations in the preservation and use of fungi or oomycetes. Fungi or oomycetes, especially filamentous fungi, need oxygen for aerobic respiration during their growth. After the common slant preservation tube stores the mycelium block on the slant, due to the sealing of the tube mouth, the mycelium block cannot effectively grow new mycelium to cover the slant, or the mycelium on the mycelium block dies due to lack of oxygen in the short term, and the possibility of contamination by anaerobic microorganisms such as bacteria is also greatly increased. In addition, since the inner wall of the slant preservation tube is relatively smooth, it is easy to cause the solid slant culture medium to slide during use, which is inconvenient for the subsequent use of the bacteria.

[0004] When preparing a sloped storage tube in the laboratory, it is necessary to melt the solid culture medium first, then add the melted culture medium to the empty tube in a sterile environment, and then place it at an angle. When the culture medium in the tube is cooled and formed, the bacteria can be inoculated on the slope. During the inclined placement of the culture medium, due to the error in the amount of culture medium added, it is easy to cause the culture medium to flow to the tube mouth, which is easy to cause pollution in later use. In addition, it is also necessary to find and control a suitable tilt angle to prevent the inclined surface area from being too small due to insufficient tilt angle, or the culture medium from flowing out due to too large tilt angle. These processes are not only time-consuming, but also prone to operational errors, resulting in waste of culture medium and consumables. Utility Model Content

[0005] In view of the deficiencies in the above-mentioned prior art, the purpose of the utility model is to provide a culture slant preservation tube and a cooling bracket thereof, which can improve the preparation efficiency of the slant preservation tube and increase the survival rate and preservation time of fungi or oomycetes during low-temperature slant preservation.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] A bacterial culture bevel storage tube comprises a tube body and a tube cover, wherein: the inner bottom surface of the tube body and one side of the inner tube wall are frosted annular surfaces, the other side of the inner tube wall is a transparent light tube for convenient observation of the growth of the internal bacterial culture, and a crescent-shaped barrier is provided on the upper part of the frosted surface of the inner tube wall; the tube cover comprises a hollow cylindrical cover body and a tube plug, and the tube plug is connected to the cover body by a connecting belt, and a semi-transparent membrane is provided in the hollow cavity of the cover body.

[0008] The frosted annular surface occupies half of the circumferential surface of the tube wall.

[0009] The crescent-shaped barrier sheet is integrally formed with the tube body, and is 1 / 5 to 1 / 3 of the tube body length away from the tube mouth section.

[0010] The arc length of the crescent-shaped barrier is the same as the arc length of the frosted annular surface.

[0011] The open end of the tube body is provided with an external thread, the lower part of the hollow inner ring surface of the tube cover is provided with an internal thread adapted to the external thread; the upper part of the hollow inner ring surface of the tube cover is smooth and adapted to the pipe plug. The pipe plug is a rubber plug.

[0012] The utility model also provides a cooling bracket for use with the above-mentioned inclined culture storage tube, comprising a cooling panel, a hinge shaft, a base and an adjusting rod. The cooling panel is arranged at an angle, and a plurality of storage tube placement grooves are opened on the panel. The lower edge of the cooling panel is connected to the base through the hinge shaft, and the inclination angle of the panel relative to the base is adjusted by an adjusting rod arranged between the base and the cooling panel.

[0013] The cooling panel is made of metal material, which can effectively dissipate heat and promote the solidification of the culture medium in the inclined preservation tube; the base is made of wood or plastic material.

[0014] Compared with the prior art, the utility model has the following advantages:

[0015] 1. Compared with the existing inclined storage tube, one side and the bottom of the tube body of the utility model are frosted, which has an anti-slip and fixing function, and the inclined solid culture medium will not slide or fall off during the inoculation or retrieval of bacteria.

[0016] 2. A crescent-shaped barrier sheet is arranged inside the inclined surface storage tube of the utility model, which can effectively prevent the internal culture medium from easily overflowing when the inclined surface is made.

[0017] 3. The tube cover of the utility model is provided with a rubber stopper and a semipermeable membrane. After the bacteria are inoculated, the cover body can be tightened first, and the rubber stopper can be opened to allow the bacteria to be expanded to a certain extent in the inclined storage tube, and then the rubber stopper can be covered and sealed, and stored at a low temperature, which effectively improves the preservation activity and storage time of the bacteria.

[0018] 4. The cooling panel of the utility model is made of metal material, which can effectively dissipate heat and promote the solidification of the culture medium in the inclined storage tube.

[0019] 5. The tilt angle of the cooling panel is adjustable, which is convenient for the operator to adjust according to the actual conditions such as the capacity of the tube body and the amount of culture medium added to obtain the optimal slope area. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the structure of the inclined storage tube;

[0021] Figure 2 A cross-sectional view of the tube cover body is preserved for the inclined surface;

[0022] Figure 3 It is a schematic diagram of the cooling bracket structure;

[0023] In the figure: 1 is a tube body, 1-1 is a crescent-shaped barrier sheet; 2 is a tube cover, 2-1 is a cover body, 2-2 is a semipermeable membrane, 2-3 is a connecting belt, 2-4 is a tube plug (which can be a rubber plug); 3 is a cooling bracket, 3-1 is a cooling panel, 3-2 is an adjusting rod, 3-3 is a hinge shaft, and 3-4 is a base. DETAILED DESCRIPTION

[0024] The utility model is further described below in conjunction with the accompanying drawings to make the technical solution of the utility model easier to understand and grasp.

[0025] like Figure 1-2 As shown: a culture culture inclined surface storage tube, including a tube body 1 and a tube cover 2, wherein: the inner bottom surface of the tube body and one side of the inner tube wall are frosted annular surfaces, the other side of the inner tube wall is a transparent light tube to facilitate observation of the internal culture culture growth, and a crescent-shaped barrier sheet 1-1 is provided on the upper part of the frosted surface of the inner tube wall; the tube cover 2 includes a hollow cylindrical cover body 2 and a tube plug 2-4, and the tube plug is connected to the cover body 2-1 through a connecting belt 2-3, and a semi-transparent membrane 2-2 is provided in the hollow cavity of the cover body 2-1.

[0026] The frosted annular surface occupies half of the circumferential surface of the tube wall.

[0027] The crescent-shaped barrier sheet 1 - 1 is integrally formed with the tube body 1 , and is 1 / 5 to 1 / 3 of the tube body length away from the tube mouth.

[0028] The arc length of the crescent-shaped barrier is the same as the arc length of the frosted annular surface.

[0029] The open end of the tube body is provided with an external thread, the lower part of the hollow inner ring surface of the tube cover is provided with an internal thread adapted to the external thread; the upper part of the hollow inner ring surface of the tube cover is smooth and adapted to the tube plug 2-4. The tube plug is a rubber plug.

[0030] like Figure 3As shown: The utility model also provides a cooling bracket 3 for use with the above-mentioned inclined culture storage tube, including a cooling panel 3-1, a hinge shaft 3-3, a base 3-4 and an adjusting rod 3-2. The cooling panel is arranged at an angle, and a plurality of storage tube placement grooves are opened on the panel. The lower edge of the cooling panel 3-1 is connected to the base 3-4 through the hinge shaft 3-3, and the inclination angle of the panel 3-1 relative to the base 3-4 is adjusted by the adjusting rod 3-2 arranged between the base and the cooling panel.

[0031] The cooling panel 3-1 is made of metal material, which can effectively dissipate heat and promote the solidification of the culture medium in the inclined preservation tube; the base 3-4 is made of wood or plastic material.

[0032] The utility model is further described below in combination with the method of use:

[0033] After taking an appropriate amount of the melted solid culture medium and adding it to the tube body 1, place it on the cooling panel 3-1 with the frosted surface facing downward, and use the adjustment rod 3-2 to adjust the cooling panel 3-1 to a suitable angle so that the culture medium forms a larger inclined surface. After the culture medium cools and solidifies, cover it with the tube cap 2 for later use. During the operation, there is no need to carefully control the amount of culture medium added. When the amount added is slightly less, the adjusting rod 3-2 can be used to reduce the angle between the cooling panel 3-1 and the base 3-4, so that the inclination of the tube body 1 is increased, and the area of ​​the inclined surface formed after the culture medium solidifies is increased; when the amount of culture medium added is slightly more, the crescent-shaped barrier sheet 1-1 can play the role of blocking the culture medium, so the adjusting rod 3-2 can be appropriately adjusted to increase the inclination angle of the tube body 1 to ensure that the area of ​​the inclined surface formed after the culture medium solidifies is large enough; after the mycelium block is inoculated into the prepared inclined surface preservation tube, the tube plug 2-4 needs to be opened after the tube cap 2 is screwed on, because the semipermeable membrane 2-2 has the function of isolating microorganisms and being breathable, so that the mycelium block is in an aerobic and sterile environment, and then placed in a suitable environment for 3 to 5 days. When the strain has multiplied to a certain extent and is relatively active, the tube plug 2-4 is covered and stored in a low temperature environment of 4 to 8°C.

[0034] Furthermore, one side and the bottom of the inner wall of the tube body 1 are frosted, and half of the upper inner wall is frosted, which has an anti-slip and fixing function, and the inclined solid culture medium will not slide or fall off during the inoculation or retrieval of the strain.

[0035] Furthermore, the other side of the inner wall of the tube is smooth and transparent, which is convenient for observing the growth of the internal bacteria.

[0036] Furthermore, the inclined surface storage tube body is provided with a crescent-shaped barrier sheet, which effectively prevents the internal culture medium from easily overflowing when the inclined surface is made. The crescent-shaped barrier sheet on the inner wall of the tube body is integrally formed with the tube body, located on the side of the frosted inner wall, and is about 1 / 5 to 1 / 3 of the tube body length away from the tube mouth.

[0037] Furthermore, the tube body is made of glass or plastic material, the cover body and the connecting belt of the tube cover are made of plastic material, the cooling panel and the hinge shaft of the cooling bracket are made of metal material, and the base is made of wood or plastic material.

[0038] Furthermore, the opening end of the tube body is provided with an external thread, the lower end of the inner wall of the tube cover body is provided with an internal thread adapted to the external thread, and the upper end of the inner wall of the cover body is smooth and adapted to the pipe plug.

[0039] Furthermore, a semi-permeable membrane is embedded in the center of the inner wall of the tube cover body, which is convenient for the expansion of initial mycelium after inoculation of the strain, and is used in conjunction with the tube plugs 2-4 to effectively improve the preservation activity and storage time of the strain.

[0040] Furthermore, the adjustment rods of the cooling bracket are located on both sides of the base, which can adjust the angle between the cooling panel and the base, making it convenient for the operator to make adjustments according to actual conditions such as the capacity of the tube body and the amount of culture medium added to obtain the optimal slope area.

Claims

1. A bacterial culture slant storage tube, comprising a tube body and a tube cover, characterized in that: The inner bottom surface of the tube body and one side of the inner tube wall are frosted annular surfaces, the other side of the inner tube wall is a transparent light tube, and a crescent-shaped barrier is provided on the upper part of the frosted surface of the inner tube wall; the tube cover includes a hollow cylindrical cover body and a tube plug, and the tube plug is connected to the cover body through a connecting belt, and a semi-transparent membrane is provided in the hollow cavity of the cover body.

2. The bacterial strain inclined surface storage tube according to claim 1, characterized in that: The frosted annular surface occupies half of the circumferential surface of the tube wall.

3. The bacterial culture slant storage tube according to claim 1, characterized in that: The crescent-shaped barrier sheet is integrally formed with the tube body, and is 1 / 5 to 1 / 3 of the tube body length away from the tube mouth section.

4. The bacterial strain slant storage tube according to claim 1 or 3, characterized in that: The arc length of the crescent-shaped barrier is the same as the arc length of the frosted annular surface.

5. The bacterial culture slant storage tube according to claim 1, characterized in that: The open end of the tube body is provided with an external thread, and the lower part of the hollow inner ring surface of the tube cover is provided with an internal thread matched with the external thread; the upper part of the hollow inner ring surface of the tube cover is smooth and matched with the pipe plug.

6. The bacterial culture slant storage tube according to claim 1, characterized in that: The tube stopper is a rubber stopper.

7. A cooling support compatible with the inclined surface storage tube for bacterial strains as described in claims 1 to 6, characterized in that: The cooling bracket includes a cooling panel, a hinge shaft, a base and an adjusting rod. The cooling panel is arranged at an angle, and a plurality of storage tube placement grooves are opened on the panel. The lower edge of the cooling panel is connected to the base through the hinge shaft, and the inclination angle of the panel relative to the base is adjusted by an adjusting rod arranged between the base and the cooling panel.

8. The cooling bracket according to claim 7, characterized in that: The cooling panel is made of metal material, which can effectively dissipate heat and promote the solidification of the culture medium in the inclined preservation tube.

9. The cooling support according to claim 7, characterized in that: The base is made of wood or plastic material.

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