Auxiliary device for detecting and screening zebra fish

Abstract

The utility model is suitable for the technical field of roe screening and collecting, and provides a zebra fish detection and screening auxiliary device which comprises a sample storage pipe, a sampling pipe, a handle, a piston and an amplification observation part, zebra fish roe screening work can be completed through the sample storage pipe, the sampling pipe, the handle, the piston and the amplification observation part, an experimenter can conveniently remove unfertilized zebra fish roes, and the screening efficiency of the zebra fish roes is improved. The problem that in the prior art, time and labor are wasted when unfertilized zebra fish eggs are removed and fertilized zebra fish eggs are transferred is solved.

Description

Technical Field

[0001] The present application belongs to the technical field of fish egg screening and collection, and in particular, relates to a zebrafish detection and screening auxiliary device. Background Art

[0002] Zebrafish and humans have 87% high homology in genes. Zebrafish skin is similar in structure to human skin, including basal layer, spinous layer, granular layer, clear layer and epidermal keratinocyte layer. The skin interstitial connective tissue, collagen and its adjacent fibroblasts and skin gene expression are similar to human skin. The evaluation of cosmetic efficacy is an important part of cosmetic development. Zebrafish has become an important in vivo model for cosmetic efficacy evaluation due to its wide range of advantages. The evaluation results obtained on zebrafish are also applicable to humans in most cases.

[0003] Zebrafish eggs are spherical in shape, with a diameter of 0.6-0.8 mm, and are usually light yellow, white, or translucent in color. Fertilized eggs are usually transparent beige or directly translucent. Unfertilized eggs are usually white.

[0004] In zebrafish egg biological experiments, it is necessary to classify, screen and collect zebrafish eggs, and remove unfertilized zebrafish embryos to avoid water pollution. The current screening work usually involves workers taking the eggs and placing them in a culture dish, using black or white paper to pad the table to initially observe the zebrafish embryos or fry, and using a magnifying glass to observe the fertilization status of the eggs. This work is inefficient and time-consuming and labor-intensive. Utility Model Content

[0005] The present application provides a zebrafish detection and screening auxiliary device, which can solve the problem that the classification, screening and collection of zebrafish eggs are time-consuming and labor-intensive.

[0006] The present application provides a zebrafish detection and screening auxiliary device, comprising:

[0007] Sample storage tube;

[0008] a sampling tube connected to the sample storage tube, the sampling tube being provided with a magnifying observation portion, the sampling tube being used to extract zebrafish eggs from the outside and to deliver the zebrafish eggs into the sample storage tube for storage, the magnifying observation portion being used to observe the zebrafish eggs passing through the magnifying observation portion; and

[0009] A handle and a piston, wherein the piston is slidably arranged in the sample storage tube along the length direction of the sample storage tube, and one end of the handle extends into the sample storage tube and is connected with the piston.

[0010] Optionally, the magnifying observation portion includes a convex lens, which is disposed on the sampling tube and is used to magnify the image of the zebrafish eggs.

[0011] Optionally, the magnifying observation portion further includes a black coating, and the black coating is disposed on a side of the sampling tube opposite to the convex lens.

[0012] Optionally, a through tube is provided on the sample storage tube, one end of the handle passes through the through tube and extends into the sample storage tube, a protrusion is provided on the handle, an elastic member is provided in the through tube, the elastic member is located between the sample storage tube and the protrusion, and the elastic member is used to provide an elastic force to keep the protrusion away from the sample storage tube.

[0013] Optionally, the zebrafish detection and screening auxiliary device further comprises a limiter, which is adjustable in the length direction of the through tube, and the end of the protrusion facing away from the sample storage tube abuts against the limiter under the action of the elastic member.

[0014] Optionally, the limiting member includes a limiting cylinder, which is threadedly connected to the through tube, and a limiting protrusion is arranged in the limiting cylinder to abut against an end of the protrusion facing away from the sample storage tube.

[0015] Optionally, a rotating handle is provided at one end of the limiting cylinder away from the through pipe, and an anti-slip groove is provided on the rotating handle.

[0016] Optionally, a sealing sleeve is provided circumferentially on the edge of the piston, and the sealing sleeve abuts against the inner wall of the sample storage tube.

[0017] The above solution of the present application has the following beneficial effects:

[0018] In the present application, a sampling tube in a zebrafish detection and screening auxiliary device is inserted into zebrafish eggs, and a handle is pulled outward to generate negative pressure in the sample storage tube through a piston, and zebrafish eggs are extracted from a zebrafish egg culture solution through the sampling tube, and the zebrafish eggs are sent to the sample storage tube for storage; when the zebrafish eggs pass through a magnifying observation portion provided on the sampling tube, the magnifying observation portion can magnify the image of the zebrafish eggs for observation by experimenters; in the present application, the screening of zebrafish eggs can be completed through the sample storage tube, the sampling tube, the handle, the piston and the magnifying observation portion, so that experimenters can conveniently remove unfertilized zebrafish eggs and transfer fertilized zebrafish eggs, thereby solving the problem of time-consuming and labor-intensive removal of unfertilized zebrafish eggs and transfer of fertilized zebrafish eggs in the prior art.

[0019] Other beneficial effects of the present application will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 A schematic diagram of the structure of a zebrafish detection and screening auxiliary device provided in one embodiment of the present application;

[0022] Figure 2 A cross-sectional schematic diagram of a zebrafish detection and screening auxiliary device provided in one embodiment of the present application.

[0023] [Description of Reference Numerals]

[0024] 1. Sample storage tube;

[0025] 2. Sampling tube;

[0026] 31. handle; 32. piston; 33. through pipe; 34. convex block; 35. elastic member; 36. limit member; 37. rotating handle; 38. sealing sleeve;

[0027] 4. Magnifying observation part; 41. Convex lens; 42. Black coating. DETAILED DESCRIPTION

[0028] In the following description, specific details such as specific system structures, technologies, etc. are provided for the purpose of illustration rather than limitation, so as to provide a thorough understanding of the embodiments of the present application. However, it should be clear to those skilled in the art that the present application may also be implemented in other embodiments without these specific details. In other cases, detailed descriptions of well-known systems, devices, circuits, and methods are omitted to prevent unnecessary details from obstructing the description of the present application.

[0029] It should be understood that when used in the present specification and the appended claims, the term "comprising" indicates the presence of described features, wholes, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or combinations thereof.

[0030] It should also be understood that the term “and / or” used in the specification and appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0031] As used in the specification and appended claims of this application, the term "if" can be interpreted as "when" or "uponce" or "in response to determining" or "in response to detecting", depending on the context. Similarly, the phrase "if it is determined" or "if [described condition or event] is detected" can be interpreted as meaning "uponce it is determined" or "in response to determining" or "uponce [described condition or event] is detected" or "in response to detecting [described condition or event]", depending on the context.

[0032] In addition, in the description of the present application specification and the appended claims, the terms "first", "second", "third", etc. are only used to distinguish the descriptions and cannot be understood as indicating or implying relative importance.

[0033] References to "one embodiment" or "some embodiments" etc. described in the specification of this application mean that one or more embodiments of the present application include specific features, structures or characteristics described in conjunction with the embodiment. Therefore, the statements "in one embodiment", "in some embodiments", "in some other embodiments", "in some other embodiments", etc. that appear in different places in this specification do not necessarily refer to the same embodiment, but mean "one or more but not all embodiments", unless otherwise specifically emphasized in other ways. The terms "including", "comprising", "having" and their variations all mean "including but not limited to", unless otherwise specifically emphasized in other ways.

[0034] The zebrafish detection and screening auxiliary device provided in the present application is exemplarily described below in conjunction with specific embodiments.

[0035] like Figure 1 and Figure 2 As shown, the zebrafish detection and screening auxiliary device provided in the embodiment of the present application includes a sample storage tube 1, a sampling tube 2, a handle 31 and a piston 32; the sampling tube 2 is connected to the sample storage tube 1, and an enlarged observation part 4 is provided on the sampling tube 2. The sampling tube 2 is used to extract external zebrafish eggs and send the zebrafish eggs into the sample storage tube 1 for storage, and the enlarged observation part 4 is used to observe the zebrafish eggs passing through the enlarged observation part 4; the piston 32 is slidably arranged in the sample storage tube 1 along the length direction of the sample storage tube 1, and one end of the handle 31 extends into the sample storage tube 1 and is connected to the piston 32.

[0036] In the above embodiment, after a large number of zebrafish eggs have completed in vitro fertilization in the zebrafish egg culture solution in the breeding box, the sampling tube 2 in the zebrafish detection and screening auxiliary device is pressed to exhaust and then extended into the zebrafish egg breeding box, the handle 31 is pulled outward, and a negative pressure is generated in the sample storage tube 1 through the piston 32, and zebrafish eggs are extracted from the zebrafish egg culture solution through the sampling tube 2 (it can be understood that when extracting from the zebrafish egg culture solution, the zebrafish eggs and the zebrafish egg culture solution will be extracted into the sampling tube 2 together and enter the sample storage tube 1, and the following process is the same), and the zebrafish eggs are sent to the sample storage tube 1 for storage. When the zebrafish eggs pass through the magnifying observation part 4 set on the sampling tube 2, the magnifying observation part 4 can magnify the image of the zebrafish eggs for observation by the experimenter. When the experimenter observes that the zebrafish eggs have been fertilized, the handle 31 is continued to be pulled to generate negative pressure so that the zebrafish eggs are sent to the sample storage tube 1 for storage. When the experimenter observes that the zebrafish eggs are unfertilized, he presses the handle 31 to generate positive pressure in the sample storage tube 1 through the piston 32, and pushes the zebrafish eggs out of the sampling tube 2, so that the unfertilized zebrafish eggs can be screened out.

[0037] After the screening is completed, the zebrafish egg culture solution filled in the sample storage tube 1 and the several fertilized zebrafish eggs contained therein are uniformly transferred to a designated container for storage in preparation for subsequent experimental needs.

[0038] Furthermore, when the handle 31 is pulled or pressed, the piston 32 will also slide in the sample storage tube 1 along the length direction of the sample storage tube 1 accordingly. By adjusting the stretched length of the handle 31, the volume between the piston 32 and the sample storage tube 1 can be changed, thereby controlling the total amount of zebrafish eggs and zebrafish egg culture solution extracted, so as to increase the number of fertilized zebrafish eggs entering the sample storage tube 1.

[0039] In the present application, the screening of zebrafish eggs can be completed through the sample storage tube 1, the sampling tube 2, the handle 31, the piston 32 and the magnifying observation part 4, which makes it convenient for experimenters to remove unfertilized zebrafish eggs and transfer fertilized zebrafish eggs, thereby solving the problem of time-consuming and labor-intensive removal of unfertilized zebrafish eggs and transfer of fertilized zebrafish eggs in the prior art.

[0040] In a preferred embodiment, the diameter of the front end portion of the sampling tube 2 is 1 mm to 1.2 mm, which is slightly larger than the diameter of the zebrafish eggs, so that individual zebrafish eggs can flow through the sampling tube 2 into the sample storage tube 1 in sequence, thereby allowing individual zebrafish eggs to pass through the magnifying observation part 4 in sequence, avoiding two or more parallel zebrafish eggs from passing through the magnifying observation part 4 at the same time.

[0041] The zebrafish egg culture solution is mainly used to provide a growth environment for zebrafish eggs. For example, the zebrafish egg culture solution can refer to the culture solution in the method for determining the acute toxicity of water substances to freshwater fish (zebrafish) in GB / T 13267-1991.

[0042] In one embodiment, if Figure 2 As shown, the magnifying observation part 4 includes a convex lens 41, which is arranged on the sampling tube 2, and is used to magnify the image of the zebrafish eggs.

[0043] In the above embodiment, the convex lens 41 is arranged on the sampling tube 2, and the image in the sampling tube 2 can be magnified by the convex lens 41. When the zebrafish eggs pass by, the image of the zebrafish eggs is magnified by the convex lens 41, which is convenient for the experimenter to observe the state of the zebrafish eggs to determine whether they are fertilized.

[0044] In a specific embodiment, Figure 2 As shown, the magnifying observation portion 4 further includes a black coating 42 , and the black coating 42 is disposed on a side of the sampling tube 2 opposite to the convex lens 41 .

[0045] In the above embodiment, the black coating 42 is arranged opposite to the convex lens 41. When the experimenter observes through the convex lens 41, the black coating 42 can enhance the contrast between the zebrafish eggs and the surrounding environment, which helps to highlight the morphological characteristics of the zebrafish eggs and make them easier to observe and screen.

[0046] In one embodiment, if Figure 2 As shown, a through tube 33 is provided on the sample storage tube 1, and one end of the handle 31 extends into the sample storage tube 1 after passing through the through tube 33. A protrusion 34 is provided on the handle 31, and an elastic member 35 is provided in the through tube 33. The elastic member 35 is located between the sample storage tube 1 and the protrusion 34, and the elastic member 35 is used to provide an elastic force to keep the protrusion 34 away from the sample storage tube 1.

[0047] In the above embodiment, when the experimenter extracts the zebrafish eggs, he presses the handle 31 to make the projection 34 compress the elastic member 35 so that the elastic member 35 is in a compressed state, and the piston 32 slides in the sample storage tube 1 until it abuts against one end of the sample storage tube 1 in the length direction. Then, the pressure applied to the handle 31 is slowly reduced, and the elastic member 35 gradually expands and contracts to drive the handle 31 to move through the projection 34, so that negative pressure is generated in the sample storage tube 1, thereby sucking the zebrafish eggs. When the experimenter observes that the zebrafish eggs flowing through the magnifying observation part 4 are not fertilized, the handle 31 is pressed again to squeeze out the zebrafish eggs in the sampling tube 2, and then the pressure applied to the handle 31 is slowly reduced. Until the elastic member 35 returns to the natural state, the space between the sample storage tube 1 and the piston 32 is full of zebrafish eggs and zebrafish egg culture solution, and the screening sampling work is completed. By providing the elastic member 35 and the protrusion 34 , after the sampling tube 2 is pressed to exhaust air, the handle 31 and the piston 32 can move through the rebound force to generate negative pressure, which can reduce the burden of manual operation.

[0048] The elastic member 35 is mainly used to provide elastic force to keep the protrusion 34 away from the sample storage tube 1. Exemplarily, the elastic member 35 can be a spring, an elastic ball, etc.

[0049] In a specific embodiment, Figure 2 As shown, the zebrafish detection and screening auxiliary device also includes a limiter 36 , which is adjustable in the length direction of the through tube 33 , and the end of the protrusion 34 facing away from the sample storage tube 1 abuts against the limiter 36 under the action of the elastic member 35 .

[0050] In the above embodiment, by adjusting the position of the limit member 36 in the through tube 33, the telescopic length of the elastic member 35 in the through tube 33 and the movement distance of the protrusion 34 in the through tube 33 can be changed to adjust the telescopic length of the handle 31, and then adjust the position of the piston 32 in the sample storage tube 1, thereby controlling the total amount of zebrafish egg solution and the number of zebrafish eggs stored in the storage tube 1 to achieve quantitative sampling.

[0051] In a more specific embodiment, Figure 2 As shown, the limiting member 36 includes a limiting tube, which is connected to the through tube 33 via threads, and a limiting protrusion is arranged in the limiting tube to abut against the end of the protrusion 34 away from the sample storage tube 1.

[0052] In the above embodiment, the position of the limiting protrusion in the limiting cylinder in the through tube 33 is precisely adjusted by rotating the limiting cylinder, thereby controlling the distance between the limiting protrusion and the storage tube 1, so as to change the telescopic length of the elastic member 35 in the through tube 33 and the movement distance of the protrusion 34 in the through tube 33, thereby realizing quantitative sampling.

[0053] Among them, a through hole can also be set on the limiting cylinder, and a plurality of through holes are arranged at intervals along the length direction of the through tube 33. The limiting cylinder is moved along the length direction of the through tube 33 so that the through hole on the limiting cylinder moves to the through hole at the corresponding position on the through tube 33, and the pin is inserted to achieve limiting.

[0054] In a specific embodiment, Figure 2 As shown, a rotating handle 37 is provided at one end of the limiting cylinder away from the through pipe 33, and an anti-slip groove is provided on the rotating handle 37.

[0055] In the above embodiment, the rotating handle 37 is convenient for the experimenter to rotate the limit cylinder to a specified position in the through pipe 33 through the threaded structure. The rotating handle 37 is provided with an anti-slip groove to increase the friction and grip of the rotating handle 37 to prevent sliding during operation.

[0056] In a specific embodiment, Figure 2 As shown, a sealing sleeve 38 is circumferentially provided on the edge of the piston 32 , and the sealing sleeve 38 abuts against the inner wall of the sample storage tube 1 .

[0057] In the above embodiment, the sealing sleeve 38 is provided to ensure effective sealing between the piston 32 and the side wall of the sample storage tube 1 during the movement.

[0058] The above is a preferred embodiment of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles described in the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. A zebrafish detection and screening auxiliary device, characterized in that: include: Sample storage tube; a sampling tube connected to the sample storage tube, the sampling tube being provided with a magnifying observation portion, the sampling tube being used to extract zebrafish eggs from outside and to deliver the zebrafish eggs into the sample storage tube for storage, the magnifying observation portion being used to observe the zebrafish eggs passing through the magnifying observation portion; and A handle and a piston, wherein the piston is slidably disposed in the sample storage tube along the length direction of the sample storage tube, and one end of the handle extends into the sample storage tube and is connected to the piston.

2. The zebrafish detection and screening auxiliary device according to claim 1, characterized in that: The magnifying observation part comprises a convex lens, which is arranged on the sampling tube and is used to magnify the image of the zebrafish eggs.

3. The zebrafish detection and screening auxiliary device according to claim 2, characterized in that: The magnifying observation portion further comprises a black coating, and the black coating is arranged on a side of the sampling tube opposite to the convex lens.

4. The zebrafish detection and screening auxiliary device according to claim 1, characterized in that: The sample storage tube is provided with a through tube, one end of the handle passes through the through tube and extends into the sample storage tube, the handle is provided with a protrusion, an elastic member is provided in the through tube, the elastic member is located between the sample storage tube and the protrusion, and the elastic member is used to provide an elastic force to keep the protrusion away from the sample storage tube.

5. The zebrafish detection and screening auxiliary device according to claim 4, characterized in that: The zebrafish detection and screening auxiliary device also includes a limiter, which is adjustable in the length direction of the through tube, and the end of the protrusion facing away from the sample storage tube abuts against the limiter under the action of the elastic member.

6. The zebrafish detection and screening auxiliary device according to claim 5, characterized in that: The limiting member comprises a limiting cylinder, the limiting cylinder is connected to the through pipe via a thread, and a limiting protrusion is arranged in the limiting cylinder and abuts against an end of the protrusion away from the sample storage tube.

7. The zebrafish detection and screening auxiliary device according to claim 6, characterized in that: A rotating handle is arranged at one end of the limiting cylinder away from the through pipe, and an anti-slip groove is arranged on the rotating handle.

8. The zebrafish detection and screening auxiliary device according to claim 1, characterized in that: A sealing sleeve is circumferentially arranged on the edge of the piston, and the sealing sleeve abuts against the inner wall of the sample storage tube.

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