Cell culture device for cell invasion experiment

Abstract

The utility model discloses a cell culture device for a cell invasion experiment, which relates to the technical field of cell invasion experiment equipment and comprises a square lower chamber, a small chamber suspended in the lower chamber and provided with an opening at the lower end, a membrane ring detachably arranged at the bottom in the small chamber, and a polycarbonate membrane fixedly sealed in the membrane ring, and the film cutting ring is arranged below the film ring. According to the utility model, the membrane ring is arranged at the bottom of the small chamber and is detachably connected with the bottom of the small chamber through the first ring groove, so that the membrane ring can be taken down to facilitate glue scraping in an experiment observation stage, and the polycarbonate membrane can also be directly placed on an observation table to be observed.

Description

Technical Field

[0001] The utility model relates to the technical field of cell invasion experiments, in particular to a cell culture device used for cell invasion experiments. Background Art

[0002] Transwell technology mainly uses cell invasion chambers (i.e. Transwell chambers, also called Transwell chambers or Transwell inserts). The Transwell chamber 2 has the shape of a small cup that can be placed in a well plate. The bottom of the cup has a semipermeable membrane, and the pore size of the semipermeable membrane is 0.1 to 12 microns. When the Transwell chamber is used, it is placed in a culture plate. The chamber is called the upper chamber, and the culture plate is called the lower chamber. The upper chamber contains the upper culture fluid, and the lower chamber contains the lower culture fluid. The upper and lower culture fluids are separated by a semipermeable membrane. The cells are planted in the upper chamber, and the components in the lower culture fluid in the lower chamber affect the cells in the upper chamber. The cells migrate from the upper chamber to the lower chamber and adhere to the semipermeable membrane at the bottom of the upper chamber. When observing the experimental results, the matrix glue needs to be scraped off, and the semipermeable membrane will be cut off for the convenience of observation.

[0003] Most of the current chambers are cup-shaped with a semipermeable membrane sealed at the bottom. When scraping the matrix gel, it should be scraped from the top to the bottom. Improper force in this process will cause the semipermeable membrane to become concave and convex and deformed, affecting the results of subsequent photo detection. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model provides a cell culture device for cell invasion experiments, which solves the problems raised in the above-mentioned background technology.

[0005] To achieve the above objectives, the utility model is implemented through the following technical solutions: a cell culture device for cell invasion experiments, comprising: a square lower chamber, a small chamber suspended in the lower chamber and open at the lower end, a detachable membrane ring arranged at the bottom of the small chamber, a polycarbonate membrane sealed in the membrane ring, and a membrane cutting ring arranged under the membrane ring.

[0006] Preferably, the membrane ring is a first circular ring, and the upper bottom surface of the first circular ring is provided with a first ring groove which is interference fit with the lower end of the chamber.

[0007] Preferably, the polycarbonate film is provided on the inner wall of the first ring.

[0008] Preferably, the film cutting ring is a second circular ring, the upper bottom surface of the second circular ring is provided with a raised third circular ring, the upper bottom surface of the second circular ring is provided with a fourth circular ring concentric with the third circular ring, the inner wall of the third circular ring and the outer wall of the fourth circular ring form a second circular groove for plugging with the first circular ring; a blade ring is provided on the bottom surface of the fourth circular ring.

[0009] Preferably, the outer side wall of the first circular ring is provided with an external thread, and the second annular groove is provided with an internal thread matching the external thread.

[0010] Preferably, grid lines are provided on the side walls of the lower chamber.

[0011] Preferably, a first support ring is provided at the upper end of the small chamber, and a bracket is provided at the lower chamber, the bracket includes a second support ring which is sleeved under the first support ring, and a circular array of support rods are provided on the side wall of the second support ring and connected to the four walls of the lower chamber, and the support rods are provided with a bayonet that is clamped on the side wall of the lower chamber.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] 1. The cell culture device for cell invasion experiment is provided with a membrane ring at the bottom of the chamber, and the membrane ring is detachably connected to the bottom of the chamber through a first ring groove. During the experimental observation stage, the membrane ring can be removed for convenient glue scraping, or the polycarbonate membrane can be directly placed on the observation table for observation.

[0014] 2. When the polycarbonate film needs to be cut, the polycarbonate film can be brought into contact with the blade ring of the film cutting ring by moving the film ring downward, so that the blade ring can cut the polycarbonate film. This is more convenient and safer than cutting with scissors or knives, and the integrity of the polycarbonate film is also better. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the structure of the cell culture device used for cell invasion experiment;

[0016] Figure 2 This is the explosion effect diagram of the small room.

[0017] In the figure: lower chamber 1, small chamber 2, membrane ring 3, polycarbonate membrane 4, film cutting ring 5, first ring groove 6, second circular ring 7, third circular ring 8, fourth circular ring 9, second ring groove 10, rubber ring 11, blade ring 12, first support ring 13, bracket 14, second support ring 15. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0019] See also Figure 1 to Figure 2This embodiment provides a cell culture device for cell invasion experiment, comprising: a square lower chamber 1, a small chamber 2 suspended in the lower chamber 1 and open at the lower end, a detachable membrane ring 3 arranged at the bottom of the small chamber 2, a polycarbonate membrane 4 sealed in the membrane ring 3, and a membrane cutting ring 5 arranged under the membrane ring 3. The small chamber 2 is cup-shaped. The membrane ring 3 can be disassembled together with the polycarbonate membrane 4, which is convenient for observation and scraping glue. The membrane cutting ring 5 is used to cut off the polycarbonate membrane 4.

[0020] The membrane ring 3 is a first circular ring, and the upper bottom surface of the first circular ring is provided with a first annular groove 6 which is interference fit with the lower end of the small chamber 2. The material in the small chamber 2 will not be mixed with the lower chamber 1 through the gap between the membrane ring 3 and the small chamber 2. A rubber ring 11 can be further added to the bottom of the first annular groove 6 to improve the sealing performance.

[0021] The inner wall of the first ring is provided with the polycarbonate membrane 4. The pore size of the polycarbonate membrane 4 is selected according to the type of cells.

[0022] The film cutting ring 5 is a second circular ring 7, the upper bottom surface of the second circular ring 7 is provided with a raised third circular ring 8, the upper bottom surface of the second circular ring 7 is provided with a fourth circular ring 9 concentric with the third circular ring 8, the inner wall of the third circular ring 8 and the outer wall of the fourth circular ring 9 form a second circular groove 10 plugged with the first circular ring; a blade ring 12 is provided on the bottom surface of the fourth circular ring 9. The first circular ring does not contact the bottom of the second circular groove 10, and the polycarbonate film 4 is contacted downward with the blade ring 12 by pressing the first circular ring downward, and the polycarbonate film 4 is separated from the inner wall of the first circular ring.

[0023] The outer wall of the first circular ring is provided with an external thread, and the second annular groove 10 is provided with an internal thread matched with the external thread. The polycarbonate film 4 is uniformly contacted with the blade ring 12 by rotating the thread and the polycarbonate film 4 is cut off.

[0024] Grid lines are arranged on the side wall of the lower chamber 1. The lower chamber 1 is transparent, and the cells in the lower chamber 1 can be conveniently counted through the grid lines.

[0025] The upper end of the small chamber 2 is provided with a first support ring 13, and the lower chamber 1 is provided with a bracket 14, the bracket 14 includes a second support ring 15 sleeved under the first support ring 13, and the side wall circumferential array of the second support ring 15 is provided with support rods 16 connected to the four walls of the lower chamber 1, and the support rods 16 are provided with bayonet holes clamped on the side walls of the lower chamber 1. The bracket 14 allows the small chamber 2 to be suspended in the lower chamber 1.

[0026] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture device for cell invasion experiment, characterized in that: include: A square lower chamber (1), a small chamber (2) suspended in the lower chamber (1) and open at the lower end, a membrane ring (3) detachably arranged at the bottom of the small chamber (2), a polycarbonate membrane (4) sealed in the membrane ring (3), and a membrane cutting ring (5) arranged under the membrane ring (3).

2. A cell culture device for cell invasion experiment according to claim 1, characterized in that: The membrane ring (3) is a first circular ring, and the upper bottom surface of the first circular ring is provided with a first annular groove (6) which is interference-fitted with the lower end of the small chamber (2).

3. A cell culture device for cell invasion experiment according to claim 2, characterized in that: The polycarbonate film (4) is provided on the inner wall of the first circular ring.

4. A cell culture device for cell invasion experiment according to claim 3, characterized in that: The film cutting ring (5) is a second circular ring (7), the upper bottom surface of the second circular ring (7) is provided with a raised third circular ring (8), the upper bottom surface of the second circular ring (7) is provided with a fourth circular ring (9) concentric with the third circular ring (8), the inner wall of the third circular ring (8) and the outer wall of the fourth circular ring (9) form a second circular groove (10) plugged into the first circular ring; a blade ring (12) is provided on the bottom surface of the fourth circular ring (9).

5. A cell culture device for cell invasion experiment according to claim 4, characterized in that: The outer wall of the first circular ring is provided with an external thread, and the second annular groove (10) is provided with an internal thread matching the external thread.

6. The cell culture device for cell invasion experiment according to claim 1, characterized in that: Grid lines are provided on the side walls of the lower chamber (1).

7. The cell culture device for cell invasion experiment according to claim 1, characterized in that: A first support ring (13) is provided at the upper end of the small chamber (2), and a bracket (14) is provided on the lower chamber (1). The bracket (14) comprises a second support ring (15) which is sleeved under the first support ring (13). A support rod (16) is provided in a circumferential array on the side wall of the second support ring (15) and is connected to the four walls of the lower chamber (1). The support rod (16) is provided with a bayonet which is clamped on the side wall of the lower chamber (1).

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