Soil microorganism fumigation device

Abstract

The utility model provides a soil microorganism fumigating device. The soil microorganism fumigating device comprises a sealing tank connected with a pressure release valve, and a vacuum device connected with the sealing tank, the kit further comprises a plurality of reagent vessels, a first container and a second container; the plurality of reagent vessels are arranged in the sealing tank and are used for accommodating fluid; each reagent vessel is connected with a liquid outlet pipe, and each liquid outlet pipe is connected with a liquid outlet valve; the first container and the second container are arranged outside the sealing tank and are communicated with one or more reagent vessels. According to the soil microorganism fumigation device, a large number of soil samples can be treated each time, meanwhile, the fumigation device is not prone to being affected by the external environment temperature, the fumigation effect is remarkable, chloroform is added and discharged in a closed environment, and the risk that chloroform is inhaled by mistake is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of soil microorganism detection, and more specifically relates to a soil microorganism fumigation device. Background Art

[0002] Soil microorganisms are the driving force for the transformation of soil organic matter and soil nutrients, and are involved in various biochemical processes such as the decomposition of organic matter, the formation of humus, and the transformation and circulation of nutrients; therefore, determining the content of microorganisms in the soil is extremely important for better grasping and understanding the main factors affecting soil health. Among them, soil fumigation is a common method for determining the content of microbial organic carbon in soil. After the soil is fumigated with chloroform, the microorganisms in the soil will be killed by chloroform, and the dead microbial cells will lyse and release the organic carbon contained in the cells. Subsequently, an organic carbon automatic analyzer is used to determine the organic carbon content in the soil after fumigation. By comparing the content of organic carbon in the soil before and after fumigation, the organic carbon content contained in the microorganisms in the soil can be obtained.

[0003] At present, the sealed tank of the existing fumigation device mostly adopts a structure with an upper end opening; a reagent dish for holding chloroform is arranged inside the sealed tank, a cover body is connected to the upper end of the sealed tank, and the sealed tank is also connected to a valve for controlling the opening / closing of the internal space and connected to a vacuum pump through a pipeline. When the fumigation device is used, firstly, the fresh soil sample is put into a beaker, and then the beaker is placed inside from the opening at the upper end of the sealed tank; then, the cover body is closed on the upper end of the sealed tank, and the joint is sealed; finally, the valve and the vacuum pump are opened to discharge the gas inside the sealed tank, and since the air pressure inside the sealed tank is reduced, the boiling point of the chloroform in the reagent dish is reduced and it starts to boil; after the chloroform boils for a period of time, the valve and the vacuum pump are closed, the cover body is opened, and the reagent dish and the beaker are recovered.

[0004] The inventors have found that if the chloroform is not completely discharged when the cover is opened, an unexpected situation may occur in which the chloroform escapes from the sealed tank and is inhaled by the operator, resulting in a technical problem of reduced safety of the soil fumigation operation. Utility Model Content

[0005] The purpose of the present application is to provide a soil microbial fumigation device to solve the technical problem in the prior art that when the cover is opened, if the chloroform is not completely discharged, the chloroform will escape from the tank and be inhaled by the operator, resulting in reduced safety of the soil fumigation operation.

[0006] To achieve the above-mentioned purpose, the technical solution adopted in the present application is: to provide a soil microorganism fumigation device, the soil microorganism fumigation device comprises a sealed tank connected with a pressure relief valve, and a vacuum device connected to the sealed tank; characterized in that it also comprises:

[0007] A plurality of reagent vessels are arranged in the sealed tank, and each of them adopts a structure with a hollow interior and an opening facing upward; each of the reagent vessels is used to contain a fluid, and each of them is connected to a liquid outlet pipe for discharging the internal fluid, passing through the sealed tank and extending out; and each of the liquid outlet pipes is connected to a liquid outlet valve;

[0008] A first container, disposed outside the sealed tank and connected to one or more of the reagent vessels; and

[0009] A second container, disposed outside the sealed tank and connected to one or more of the reagent vessels;

[0010] The first container is used for containing and inputting chloroform into the reagent dish, and the second container is used for containing and inputting sodium hydroxide into the reagent dish.

[0011] Preferably, the first container and the second container are both connected to the corresponding reagent dish via a liquid inlet pipe; and each of the liquid inlet pipes is connected to a liquid inlet valve.

[0012] Preferably, the reagent dish adopts a tapered structure that is thick at the top and thin at the bottom; and the liquid outlet pipe is connected to the lower end surface of the reagent dish.

[0013] Preferably, the sealed tank comprises:

[0014] The tank body has a hollow interior and an open top; and

[0015] A cover body, disposed on the tank body and used to close the opening of the tank body;

[0016] Wherein, the cover body is provided with an air extraction valve, and the air extraction valve is connected to the vacuum device.

[0017] Preferably, an annular sealing member is connected to the opening of the tank body; when the cover body closes the opening of the tank body, the sealing member fills the gap between the opening of the tank body and the cover body.

[0018] As preferably, it also includes:

[0019] A constant temperature component, disposed inside the sealed tank, for maintaining a constant temperature inside the sealed tank; and

[0020] The sample dish is arranged between the constant temperature component and the reagent dish, and is supported on the inner bottom surface of the sealed tank through a shelf.

[0021] Preferably, the thermostatic component comprises:

[0022] a temperature control unit, disposed on the inner bottom surface of the sealed can, having a temperature-raising state capable of raising the temperature inside the sealed can, and a temperature-lowering state capable of lowering the temperature inside the sealed can; and

[0023] A sensor, disposed on the sealed can, for monitoring the internal temperature of the sealed can;

[0024] The signal output module of the sensor is electrically connected to the signal input module of the temperature control unit; the temperature control unit can be switched to the temperature rising state or the temperature falling state through the output signal of the sensor.

[0025] Preferably, the reagent dish and the sample dish are both located on the upper side of the temperature control unit, and a plurality of temperature conducting holes are provided on the storage rack.

[0026] Preferably, there are a plurality of sample dishes; a tray for accommodating the plurality of sample dishes is disposed on the shelf, and a plurality of spacers are disposed on the tray to separate the spaces on the tray.

[0027] Preferably, the sealed tank is connected with a vacuum gauge for monitoring the internal pressure value.

[0028] The soil microorganism fumigation device provided by the present application can input chloroform into one or more reagent dishes through the first container, and then form a negative pressure environment inside the tank body through a vacuum device to make the chloroform in one or more reagent dishes boil, so that the soil sample placed in the tank body can be effectively fumigated; after the fumigation operation, the residual liquid chloroform can be output to the outside of the tank body by opening the corresponding liquid outlet valve, and at the same time, sodium hydroxide (or other liquid substances that can chemically react with chloroform) can be input into one or more reagent dishes through the second container to make the residual gaseous chloroform react and eliminate it.

[0029] Compared with the prior art, the soil microorganism fumigation device provided in the present application can process a large amount of soil samples each time, is not easily affected by the external ambient temperature, has a significant fumigation effect, and the addition and discharge of chloroform are both in a closed environment, reducing the risk of accidental inhalation of chloroform. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0031] Figure 1A schematic diagram of the explosion structure of the soil microorganism fumigation device provided in an embodiment of the present application;

[0032] Figure 2 A schematic diagram of the three-dimensional structure of a soil microbial fumigation device provided in an embodiment of the present application;

[0033] Figure 3 This is a schematic diagram of the cross-sectional structure of the soil microbial fumigation device provided in an embodiment of the present application.

[0034] Among them, the reference numerals in the figure are:

[0035] 1. Sealed tank; 11. Tank body; 12. Cover body; 13. Sealing member; 14. Pressure relief valve; 15. Air extraction valve; 2. Reagent dish; 21. Liquid outlet pipe; 22. Liquid inlet pipe; 23. Liquid outlet valve; 24. Liquid inlet valve; 3. First container; 4. Second container; 5. Constant temperature assembly; 51. Temperature control unit; 52. Sensor; 6. Storage rack; 61. Temperature conducting hole; 7. Sample dish; 71. Tray; 72. Spacer; 8. Vacuum gauge; 9. Vacuum device. DETAILED DESCRIPTION

[0036] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0037] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0038] It should be understood that the orientation or position relationship indicated by terms such as "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0039] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0040] Please also read Figures 1 to 3 The soil microorganism fumigation device provided by the present application is now described. The soil microorganism fumigation device comprises a sealed tank 1, a vacuum device 9, a plurality of reagent dishes 2, a first container 3 and a second container 4.

[0041] The sealed tank 1 is made of stainless steel and has high temperature and high pressure resistance, which reduces the problem of easy explosion of traditional fumigation containers in vacuum.

[0042] A pressure relief valve 14 is connected to the sealed can 1; when the internal pressure of the sealed can 1 exceeds the pressure set by the pressure relief valve 14, the pressure relief valve 14 is opened to release the pressure, thereby ensuring the safety of the sealed can 1 when in use; the pressure relief valve 14 can adopt a manual control mode or an automatic control mode. When the fumigation is completed, the negative pressure state inside the sealed can 1 can be restored to atmospheric pressure by opening the pressure relief valve 14.

[0043] The vacuum device 9 is connected to the sealed tank 1, and its structure includes a vacuum pump and a pipeline connected to the sealed tank 1; by turning on the vacuum pump, the air inside the sealed tank 1 can be extracted from the pipeline, thereby forming a negative pressure environment in the sealed tank 1.

[0044] A plurality of reagent dishes 2 are arranged inside the sealed tank 1; each reagent dish 2 has a hollow interior and an upward opening, and is made of corrosion-resistant materials.

[0045] The first container 3 is disposed outside the sealed tank 1 , and the first container 3 is connected to one or more reagent dishes 2 , so that an operator can input the reagent in the first container 3 into the corresponding reagent dish 2 .

[0046] The second container 4 is disposed outside the sealed tank 1 , and the second container 4 is connected to one or more reagent dishes 2 . An operator can input the reagent in the second container 4 into the corresponding reagent dish 2 .

[0047] The first container 3 is used to contain and input chloroform into one or more reagent dishes 2, and the second container 4 is used to contain and input sodium hydroxide into one or more reagent dishes 2. Sodium hydroxide can be replaced by other liquid solutions that can react chemically with chloroform.

[0048] The soil microorganism fumigation device provided in the present application can input chloroform into one or more reagent dishes 2 through the first container 3, and then form a negative pressure environment inside the tank body 11 through the vacuum device 9, so that the chloroform in one or more reagent dishes 2 boils, so that the soil sample placed in the tank body 11 can be effectively fumigated; after the fumigation operation, the residual liquid chloroform can be output to the outside of the tank body 11 by opening the corresponding liquid outlet valve 23, and at the same time, sodium hydroxide (or other liquid substances that can chemically react with chloroform) can be input into one or more reagent dishes 2 through the second container 4, so that the residual gaseous chloroform reacts and is eliminated.

[0049] Compared with the prior art, the soil microorganism fumigation device provided in the present application can process a large amount of soil samples each time, is not easily affected by the external ambient temperature, has a significant fumigation effect, and the addition and discharge of chloroform are both in a closed environment, reducing the risk of accidental inhalation of chloroform.

[0050] In some embodiments, please refer to Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, the first container 3 and the second container 4 are both connected to a liquid inlet pipe 22, and each liquid inlet pipe 22 is connected to a corresponding reagent dish 2; and each liquid inlet pipe 22 is connected to a liquid inlet valve 24, and the liquid inlet valve 24 is used to control the volume of the reagent input from the first container 3 or the second container 4 into the reagent dish 2.

[0051] The liquid inlet valve 24 can be a solenoid valve to achieve the technical purpose of remotely controlling the opening and closing of the liquid inlet pipe 22. The significance of such a design is that during the experiment, the operator can stay away from the fumigation experiment position, thereby avoiding the risk of inhaling chloroform and ensuring the personal safety of the operator. In order to save costs, the liquid inlet valve 24 can also be a manual valve, and the technical purpose of controlling the opening and closing of the liquid inlet pipe 22 can be achieved by manual control.

[0052] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, the reagent dish 2 adopts a conical structure with a thick upper part and a thin lower part, and the liquid outlet pipe 21 is connected to the lower end surface of the reagent dish 2.

[0053] By adopting the above technical solution, when the reagent in the reagent dish 2 is discharged through the liquid outlet pipe 21, chloroform or other reagents are not easy to remain on the inner wall of the reagent dish 2 due to the inclined inner wall, thereby achieving the technical purpose of reducing the chemical residues in the reagent dish 2.

[0054] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, the sealed tank 1 includes a tank body 11 and a cover body 12.

[0055] The sealed can 1 has a structure with a hollow interior and an open upper end; the cover 12 is disposed on the can body 11 to seal the upper opening of the can body 11 .

[0056] Among them, the cover body 12 is provided with an exhaust valve 15, and the exhaust valve 15 is connected to the vacuum device 9; the exhaust valve 15 is a one-way valve, and the gas inside the sealed tank 1 can only be discharged to the outside through the exhaust valve 15. When the vacuum device 9 stops exhausting, the outside air cannot enter the sealed tank 1 through the exhaust valve 15, providing a stable vacuum environment, so that the chloroform can boil stably, ensuring the fumigation quality.

[0057] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided by the present application, an annular sealing member 13 is connected to the opening of the tank body 11, and the sealing member 13 is a rubber ring or a silicone ring; when the cover body 12 closes the upper opening of the tank body 11, the sealing member 13 fills the gap between the tank body 11 and the cover body 12 to ensure the vacuum environment inside the sealed tank 1 during fumigation and prevent chloroform gas from escaping from the gap after fumigation.

[0058] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, the soil microorganism fumigation device also includes a constant temperature component 5 and a sample dish 7.

[0059] The thermostatic component 5 is disposed inside the sealed can 1 . The thermostatic component 5 can preset a temperature value so that the temperature inside the sealed can 1 is stabilized at a preset temperature.

[0060] The sample dish 7 is arranged between the constant temperature assembly 5 and the reagent dish 2. The sample dish 7 is supported on the inner bottom surface of the sealed tank 1 through the storage rack 6. There can be multiple sample dishes 7 for containing and transferring the soil to be tested.

[0061] In some embodiments, see Figures 1 to 3 As a specific implementation of the soil microorganism fumigation device provided in the present application, the constant temperature component 5 includes a temperature control unit 51 and a sensor 52.

[0062] The temperature control unit 51 is arranged on the inner bottom surface of the sealed can 1, and has a cooling semiconductor and a heating semiconductor inside. The temperature control component has a heating state that can increase the temperature inside the sealed can 1, and a cooling state that can reduce the temperature inside the sealed can 1.

[0063] The sensor 52 is disposed on the sealed can 1 , and may also be disposed inside the sealed can 1 . The sensor 52 is used to monitor the internal temperature of the sealed can 1 .

[0064] Among them, the signal output module of the sensor 52 is electrically connected to the signal input module of the temperature control unit 51. Through the output signal of the sensor 52, the temperature control unit 51 can be switched to a heating state or a cooling state. Specifically: the sensor 52 can preset a temperature. When the sensor 52 detects that the temperature inside the sealed can 1 is lower than the preset temperature, the sensor 52 outputs a signal to switch the temperature control unit 51 to a heating state, the heating semiconductor is turned on, and the cooling semiconductor is turned off until the preset temperature is reached; when the sensor 52 detects that the temperature inside the sealed can 1 is higher than the preset temperature, the sensor 52 outputs a signal to switch the temperature control unit 51 to a cooling state, the cooling semiconductor is turned on, and the heating semiconductor is turned off until the preset temperature is reached.

[0065] The stability of the temperature inside the sealed tank 1 can ensure that the chloroform will not boil due to excessively high temperature, causing the chloroform to splash out of the reagent dish 2 and making it difficult to handle the chloroform; it can also ensure that the chloroform will not fail to boil completely due to excessively low temperature, reducing the fumigation effect and making it impossible to guarantee the accuracy of the experimental data.

[0066] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, the reagent dish 2 and the sample dish 7 are both located on the upper side of the temperature control unit 51, and a plurality of temperature conducting holes 61 are provided on the rack 6, and the temperature conducting holes 61 can be circular or other irregular shapes; the setting of the temperature conducting holes 61 avoids the body of the rack 6 blocking the temperature of the constant temperature component 5 from being transferred upward.

[0067] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided by the present application, a tray 71 for accommodating a plurality of sample dishes 7 is provided on the rack 6; a plurality of spacers 72 are provided on the tray 71, and the spacers 72 are used to separate the space on the tray 71; the sample dish 7 is arranged between two connected spacers 72. Moreover, the spacers 72 are made of elastic material, and can generate rebound force when the sample dish 7 hits.

[0068] When the tray 71 is transferred, the sample dish 7 on the tray 71 can remain stable under the restriction of the spacer 72, thereby preventing the sample dish 7 from tipping over due to shaking during the transfer.

[0069] In some embodiments, see Figures 1 to 3 As a specific embodiment of the soil microorganism fumigation device provided in the present application, a vacuum gauge 8 for monitoring the internal pressure value of the sealed tank 1 is connected to the sealed tank 1 .

[0070] The vacuum gauge 8 may be a digital pressure gauge, so that the operator can easily check the pressure value inside the sealed tank 1 ; the vacuum gauge 8 may also be an electronic pressure gauge, so that the pressure value inside the sealed tank 1 can be remotely transmitted.

[0071] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A soil microorganism fumigation device, comprising a sealed tank (1) connected to a pressure relief valve (14), and a vacuum device (9) connected to the sealed tank (1); characterized in that: Also includes: A plurality of reagent dishes (2) are arranged in the sealed tank (1) and are each hollow inside with an opening facing upward; each of the reagent dishes (2) is used to contain a fluid and is connected to a liquid outlet pipe (21) for discharging the internal fluid, passing through the sealed tank (1) and extending out; and each of the liquid outlet pipes (21) is connected to a liquid outlet valve (23); A first container (3), which is arranged outside the sealed tank (1) and is connected to one or more of the reagent vessels (2); and A second container (4), arranged outside the sealed tank (1) and connected to one or more of the reagent vessels (2); The first container (3) is used for containing and inputting chloroform into the reagent dish (2), and the second container (4) is used for containing and inputting sodium hydroxide into the reagent dish (2).

2. The soil microorganism fumigation device according to claim 1, characterized in that: The first container (3) and the second container (4) are both connected to the corresponding reagent dish (2) via a liquid inlet pipe (22); and each of the liquid inlet pipes (22) is connected to a liquid inlet valve (24).

3. The soil microorganism fumigation device according to claim 1, characterized in that: The reagent dish (2) adopts a tapered structure that is thick at the top and thin at the bottom; and the liquid outlet pipe (21) is connected to the lower end surface of the reagent dish (2).

4. The soil microorganism fumigation device according to claim 1, characterized in that: The sealed can (1) comprises: The tank body (11) has a hollow interior and an open top; and A cover (12) is arranged on the tank body (11) and is used to close the opening of the tank body (11); Wherein, the cover body (12) is provided with an air extraction valve (15), and the air extraction valve (15) is connected to the vacuum device (9).

5. The soil microorganism fumigation device according to claim 4, characterized in that: An annular sealing member (13) is connected to the opening of the tank body (11); when the cover body (12) closes the opening of the tank body (11), the sealing member (13) fills the gap between the opening of the tank body (11) and the cover body (12).

6. The soil microorganism fumigation device according to claim 1, characterized in that: Also includes: A constant temperature component (5), arranged inside the sealed can (1), and used to keep the temperature inside the sealed can (1) constant; as well as The sample dish (7) is arranged between the constant temperature component (5) and the reagent dish (2), and is supported on the inner bottom surface of the sealed tank (1) through a storage rack (6).

7. The soil microorganism fumigation device according to claim 6, characterized in that: The constant temperature component (5) comprises: a temperature control unit (51), arranged on the inner bottom surface of the sealed can (1), and having a temperature-raising state capable of raising the internal temperature of the sealed can (1), and a temperature-lowering state capable of lowering the internal temperature of the sealed can (1); and A sensor (52) is arranged on the sealed can (1) and is used to monitor the internal temperature of the sealed can (1); The signal output module of the sensor (52) is electrically connected to the signal input module of the temperature control unit (51); and the temperature control unit (51) can be switched to the temperature increase state or the temperature decrease state through the output signal of the sensor (52).

8. The soil microorganism fumigation device according to claim 7, characterized in that: The reagent dish (2) and the sample dish (7) are both located on the upper side of the temperature control unit (51), and a plurality of temperature conducting holes (61) are provided on the storage rack (6).

9. The soil microorganism fumigation device according to claim 6, characterized in that: There are a plurality of sample dishes (7); a tray (71) for accommodating the plurality of sample dishes (7) is arranged on the storage rack (6), and a plurality of spacers (72) are arranged on the tray (71) to separate the space on the tray (71).

10. The soil microorganism fumigation device according to claim 1, characterized in that: The sealed tank (1) is connected to a vacuum gauge (8) for monitoring the internal pressure value.

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