Multi-channel fluorescence detection module and detector

Abstract

The utility model discloses a multichannel fluorescence detection module and a detector, the detection module comprises a shell, an exciting light generation assembly, an exciting light transmission optical fiber and an emitted light generation assembly, and the shell is provided with a first accommodating groove and a second accommodating groove; the exciting light generation assembly is arranged in the first accommodating groove in a sliding manner and comprises a first sliding block and a plurality of exciting light generation units which are linearly arranged in the length direction of the first sliding block; the exciting light transmission optical fiber is positioned above the exciting light generation assembly and is used for transmitting a target light beam determined by the exciting light target generation unit to the reagent reaction device; the emitted light generation assembly is slidably arranged in the second accommodating groove and is used for processing the reaction light beam after correspondingly receiving the reaction light beam so as to obtain an emitted light beam. According to the scheme, the structure is small and precise, the influence of light beams of different wavebands on fluorescence can be detected in a linear motion mode, and operation is convenient.

Description

Technical Field

[0001] The utility model belongs to the technical field of reagent detection, and specifically relates to a multi-channel fluorescence detection module and a detector. Background Art

[0002] With the advancement of point-of-care medical technology, there is an increasing interest in gene analysis, in vitro diagnostics, and gene sequence analysis, and the demand for this is also increasing. Platforms and systems that quickly perform a large number of analyses using a small amount of sample have been developed and introduced to the market. For example, there is interest in microfluidic devices or platforms such as chip lab devices. Such microfluidic devices include multiple microfluidic channels and chambers to control and process small amounts of fluid. The use of microfluidic devices reduces the time period of microfluidic reactions and allows microfluidic reactions and measurements of microfluidic reactions to be performed simultaneously. Such microfluidic devices are manufactured by various methods.

[0003] For example, in gene analysis, samples can be refined / extracted and amplified to obtain a sufficient amount of sample for analyzing whether the sample has a certain DNA or accurately detecting the amount of a certain DNA. For example, among various gene amplification methods, polymerase chain reaction (PCR) is the most widely used. Fluorescence detection methods are widely used to detect DNA amplified by PCR. In the prior art, the fluorescence detection module is large in size and inconvenient to operate. Utility Model Content

[0004] In view of the deficiencies of the prior art, the purpose of the present invention is to provide a multi-channel fluorescence detection module and a detector, which solves the problems of complex structure and cumbersome operation of the detection module in the prior art.

[0005] According to one aspect of the present application, a multi-channel fluorescence detection module is disclosed, and the multi-channel fluorescence detection module includes:

[0006] A housing, wherein a first accommodating groove and a second accommodating groove are provided on the housing;

[0007] an excitation light generating component, the excitation light generating component is slidably disposed in the first containing groove, the excitation light generating component comprises a first slider and a plurality of excitation light generating units linearly arranged along the length direction of the first slider;

[0008] An excitation light transmission optical fiber, the excitation light transmission optical fiber is located above the excitation light generating component, and the excitation light transmission optical fiber is used to transmit the target light beam determined by the excitation light target generating unit to the reagent reaction device; wherein the excitation light target generating unit is any one of the multiple excitation light generating units;

[0009] An emission light generating component is slidably disposed in the second receiving groove, and is used for processing the reaction light beam after correspondingly receiving the reaction light beam to obtain an emission light beam, wherein the reaction light beam is obtained after the target light beam reacts in the reagent reaction device.

[0010] Furthermore, the emission light generating assembly includes a second slider and a plurality of emission light generating units linearly arranged along the length direction of the second slider, and the number of the emission light generating units corresponds to the number of the excitation light generating units.

[0011] Furthermore, the multi-channel fluorescence detection module also includes a conversion block, one end of the excitation light transmission optical fiber is located above the excitation light target generation unit, and the other end of the excitation light transmission optical fiber is located in the conversion block.

[0012] Furthermore, the multi-channel fluorescence detection module also includes a reaction beam transmission optical fiber, one end of which is located in the conversion block, and the other end of which is located above the emission light target generation unit, wherein the emission light target generation unit is any one of the multiple emission light generation units.

[0013] Furthermore, the multi-channel fluorescence detection module also includes a light source component, which is arranged below the excitation light generating component, and the light source component is used to provide a full-spectrum light source for the excitation light target generating unit.

[0014] Furthermore, the light source is arranged on the housing.

[0015] Furthermore, the multi-channel fluorescence detection module also includes a power component, which is respectively connected to the excitation light generating component and the emission light generating component, and the power component drives the excitation light generating component and the emission light generating component to slide synchronously.

[0016] Furthermore, a plurality of first channels corresponding to the plurality of excitation light generating units are linearly arranged on the first slider along the length direction of the first slider, and each excitation light generating unit includes a first lens and a first filter, the first lens is arranged at the bottom of the corresponding first channel, and the first filter is arranged at the top of the corresponding first channel.

[0017] Furthermore, a plurality of second channels corresponding to the plurality of emission light generating units are linearly arranged on the second slider along the length direction of the second slider, and each emission light generating unit includes a second lens, a second filter and a reflector, the second lens is arranged at the top of the corresponding second channel, the second filter is arranged at the bottom of the corresponding second channel, and the reflector is arranged between the second lens and the second filter.

[0018] According to another aspect of the present application, a detector is also disclosed, and the detector includes the multi-channel fluorescence detection module described above.

[0019] The multi-channel fluorescence detection module of the utility model includes a shell, an excitation light generating component, an excitation light transmission optical fiber and an emission light generating component, wherein the shell is provided with a first receiving groove and a second receiving groove; the excitation light generating component is slidably arranged in the first receiving groove, and the excitation light generating component includes a first slider and a plurality of excitation light generating units linearly arranged along the length direction of the first slider; the excitation light transmission optical fiber is located above the excitation light generating component, and the excitation light transmission optical fiber is used to transmit the target light beam determined by the excitation light target generating unit to the reagent reaction device; wherein the excitation light target generating unit is any one of the plurality of excitation light generating units; the emission light generating component is slidably arranged in the second receiving groove, and the emission light generating component is used to process the reaction light beam after correspondingly receiving the reaction light beam to obtain the emission light beam, wherein the reaction light beam is obtained after the reaction of the target light beam in the reagent reaction device. The structure of this scheme is compact and precise, and the detection of the influence of light beams of different wavelengths on fluorescence can be realized through linear motion, and the operation is convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0021] Figure 1 It is a structural schematic diagram of a multi-channel detection module according to an embodiment of the utility model;

[0022] Figure 2 It is a partial schematic diagram of a multi-channel detection module of an embodiment of the utility model;

[0023] Figure 3 It is a cross-sectional view at one angle of the multi-channel detection module of the embodiment of the utility model;

[0024] Figure 4 is a cross-sectional view of the multi-channel detection module of the embodiment of the utility model from another angle;

[0025] Figure 5 is a cross-sectional view of an excitation light generating assembly according to an embodiment of the utility model;

[0026] Figure 6 is a cross-sectional view of an emission light generating assembly according to an embodiment of the utility model;

[0027] In the figure, 1-shell, 11-first accommodating groove, 12-second accommodating groove, 2-excitation light generating component, 21-first slider, 211-first channel, 22-excitation light generating unit, 221-first lens, 222-first filter, 3-emission light generating component, 31-second slider, 311-second channel, 32-emission light generating unit, 321-second lens, 322-second filter, 323-reflector, 4-conversion block, 5-excitation light transmission optical fiber, 6-reaction light beam transmission optical fiber, 7-light source, 8-power component, 9-support plate, 10-cover plate, 20-signal converter. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] According to one aspect of the present application, a multi-channel fluorescence detection module is disclosed. Figures 1 to 6 The multi-channel fluorescence detection module includes a shell 1, an excitation light generating component 2, an excitation light transmission optical fiber 5 and an emission light generating component 3. A first receiving groove 11 and a second receiving groove 12 are provided on the shell 1; the excitation light generating component 2 is slidably disposed in the first receiving groove 11, and the emission light generating component 3 is slidably disposed in the second receiving groove 12. Specifically, a partition is provided between the first receiving groove 11 and the second receiving groove 12. By providing a partition between the first receiving groove 11 and the second receiving groove 12, it is possible to avoid mutual interference between the light in the excitation light generating component 2 and the light in the emission light generating component 3, thereby improving the detection effect. Furthermore, a light source component 7 is also provided on the shell 1, and the light source component 7 is installed on the shell 1 through a circuit board. The light source component 7 is located below the excitation light generating component 2, and the light source component 7 can provide a full-spectrum light source.

[0030] Furthermore, the excitation light generating component 2 includes a first slider 21 and a plurality of excitation light generating units 22 linearly arranged along the length direction of the first slider 21; the excitation light transmission optical fiber 5 is located above the excitation light generating component 2, and the excitation light transmission optical fiber 5 is used to transmit the target light beam determined by the excitation light target generating unit to the reagent reaction device. Among them, the excitation light target generating unit is any one of the plurality of excitation light generating units 22. Specifically, when the excitation light generating component 2 slides in the first receiving groove 11, it is possible to achieve the correspondence between any one of the plurality of excitation light generating units 22 and the light source part 7, and the excitation light generating unit 22 corresponding to the light source part 7 can be the current excitation light target generating unit, and the excitation light target generating unit filters the full-spectrum light source to filter out unnecessary bands to obtain a target light beam, and the target light beam is transmitted to the reagent reaction device through the excitation light transmission optical fiber 5. It is understandable that the wavelength band required by each excitation light generating unit 22 can be preset. For example, in this example, there can be 6 excitation light generating units 22, of which 4 are used for testing and 2 are used as spares. The wavelength bands required by the 4 excitation light generating units 22 can be the wavelength bands corresponding to red, yellow, blue and green light, respectively. It is understandable that the above number of excitation light generating units 22 and the setting of the wavelength bands are only an exemplary description. In other feasible schemes, they can be set according to actual needs, and are not specifically limited here. Specifically, by sliding the excitation light generating component 2 in the first accommodating groove 11, the correspondence between different excitation light generating units 22 and the light source part 7 can be achieved by sliding the excitation light generating component 2. Target light beams of different wavelength bands can be obtained when only one light source part 7 is set, and there is no need to set multiple light source parts 7. The structure of the detection module is simplified, and the slider is easy to operate.

[0031] Further, the emission light generating component 3 is slidably disposed in the second receiving groove 12, and the emission light generating component 3 is used to process the reaction light beam after receiving the reaction light beam to obtain the emission light beam. The reaction light beam is obtained based on the reaction of the target light beam in the reagent reaction device. Specifically, the emission light generating component 3 includes a second slider 31 and a plurality of emission light generating units 32 linearly arranged along the length direction of the second slider 31, and the number of the emission light generating units 32 corresponds to the number of the excitation light generating units 22. The emission light generating component 3 slides synchronously with the excitation light generating component 2.

[0032] The multi-channel fluorescence detection module of the present technical solution has a simple structure. Through a sliding operation, the switching of different excitation light generating units 22 and emission light generating units 32 can be achieved, and light beams of different bands can be filtered, thereby realizing the reaction of light beams of different bands with fluorescence. The operation is convenient and meets the requirements for detecting the effects of different lights on fluorescence.

[0033] Furthermore, the multi-channel fluorescence detection module further includes a cover plate 10, a support plate 9, a reaction beam transmission optical fiber 6, and a signal converter 20. The support plate 9 is supported on the housing 1, and the cover plate 10 is arranged on the housing 1. The cover plate 10 is located above the support plate 9, and the support plate 9 is located above the excitation light generating component 2 and the emission light generating component 3. The support plate 9 supports a conversion block 4, a first fixed block, and a second fixed block. Specifically, one end of the excitation light transmission optical fiber 5 passes through the first fixed block and the support plate 9 in sequence and is located above the excitation light target generating unit, and the other end of the excitation light transmission optical fiber 5 is located in the conversion block 4. One end of the reaction beam transmission optical fiber 6 is located in the conversion block 4, and the other end of the reaction beam transmission optical fiber 6 passes through the second fixed block and the support plate 9 in sequence and is located above the emission light target generating unit, wherein the emission light target generating unit is any one of the multiple emission light generating units 32. The signal converter 20 is used to receive the reflected light beam, convert the optical signal into an electrical signal, and then output it.

[0034] Furthermore, the multi-channel fluorescence detection module also includes a power component 8, which is connected to the excitation light generating component 2 and the emission light generating component 3 respectively, and the power component 8 drives the excitation light generating component 2 and the emission light generating component 3 to slide synchronously.

[0035] Furthermore, a plurality of first channels 211 corresponding to the plurality of excitation light generating units 22 are linearly arranged on the first slider 21 along the length direction of the first slider 21 , and each excitation light generating unit 22 includes a first lens 221 and a first filter 222 . The first lens 221 is arranged at the bottom of the corresponding first channel 211 , and the first filter 222 is arranged at the top of the corresponding first channel 211 .

[0036] Furthermore, a plurality of second channels 311 corresponding to the plurality of emission light generating units 32 are linearly arranged on the second slider 31 along the length direction of the second slider 31. Each emission light generating unit 32 includes a second lens 321, a second filter 322 and a reflector 323. The second lens 321 is arranged at the top of the corresponding second channel 311, the second filter 322 is arranged at the bottom of the corresponding second channel 311, and the reflector 323 is arranged between the second lens 321 and the second filter 322.

[0037] According to another aspect of the present application, a detector is also disclosed, and the detector includes the multi-channel fluorescence detection module mentioned above.

[0038] The multi-channel fluorescence detection module of the utility model includes a housing 1, an excitation light generating component 2, an excitation light transmission optical fiber 5 and an emission light generating component 3, wherein the housing 1 is provided with a first receiving groove 11 and a second receiving groove 12; the excitation light generating component 2 is slidably arranged in the first receiving groove 11, and the excitation light generating component 2 includes a first slider 21 and a plurality of excitation light generating units 22 linearly arranged along the length direction of the first slider 21; the excitation light transmission optical fiber 5 is located above the excitation light generating component 2, and the excitation light transmission optical fiber 5 is used to transmit the target light beam determined by the excitation light target generating unit to the reagent reaction device; wherein the excitation light target generating unit is any one of the plurality of excitation light generating units 22; the emission light generating component 3 is slidably arranged in the second receiving groove 12, and the emission light generating component 3 is used to process the reaction light beam after correspondingly receiving the reaction light beam to obtain the emission light beam, wherein the reaction light beam is obtained after the reaction of the target light beam in the reagent reaction device. The structure of this scheme is compact and precise, and the detection of the influence of light beams of different wavelengths on fluorescence can be realized through linear motion, and the operation is convenient.

[0039] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A multi-channel fluorescence detection module, characterized in that: The multi-channel fluorescence detection module comprises: A housing (1), wherein the housing (1) is provided with a first accommodating groove (11) and a second accommodating groove (12); an excitation light generating component (2), the excitation light generating component (2) being slidably disposed in the first containing groove (11), the excitation light generating component (2) comprising a first sliding block (21) and a plurality of excitation light generating units (22) linearly arranged along a length direction of the first sliding block (21); an excitation light transmission optical fiber (5), the excitation light transmission optical fiber (5) being located above the excitation light generating component (2), the excitation light transmission optical fiber (5) being used to transmit a target light beam determined by an excitation light target generating unit to a reagent reaction device; wherein the excitation light target generating unit is any one of the plurality of excitation light generating units (22); An emission light generating component (3), the emission light generating component (3) being slidably disposed in the second containing groove (12), the emission light generating component (3) being used for processing the reaction light beam after correspondingly receiving the reaction light beam to obtain an emission light beam, wherein the reaction light beam is obtained after the target light beam reacts in the reagent reaction device.

2. The multi-channel fluorescence detection module according to claim 1, characterized in that: The emission light generating component (3) comprises a second slider (31) and a plurality of emission light generating units (32) linearly arranged along the length direction of the second slider (31), and the number of the emission light generating units (32) corresponds to the number of the excitation light generating units (22).

3. The multi-channel fluorescence detection module according to claim 2, characterized in that: The multi-channel fluorescence detection module further comprises a conversion block (4), one end of the excitation light transmission optical fiber (5) is located above the excitation light target generation unit, and the other end of the excitation light transmission optical fiber (5) is located inside the conversion block (4).

4. The multi-channel fluorescence detection module according to claim 3, characterized in that: The multi-channel fluorescence detection module further comprises a reaction light beam transmission optical fiber (6), one end of the reaction light beam transmission optical fiber (6) is located in the conversion block (4), and the other end of the reaction light beam transmission optical fiber (6) is located above an emission light target generation unit, wherein the emission light target generation unit is any one of the plurality of emission light generation units (32).

5. The multi-channel fluorescence detection module according to claim 1 or 3, characterized in that: The multi-channel fluorescence detection module further comprises a light source component (7), wherein the light source component (7) is arranged below the excitation light generating component (2), and the light source component (7) is used to provide a full-spectrum light source for the excitation light target generating unit.

6. The multi-channel fluorescence detection module according to claim 5, characterized in that: The light source component (7) is arranged on the housing (1).

7. The multi-channel fluorescence detection module according to claim 1, characterized in that: The multi-channel fluorescence detection module further comprises a power component (8), wherein the power component (8) is respectively connected to the excitation light generating component (2) and the emission light generating component (3), and the power component (8) drives the excitation light generating component (2) and the emission light generating component (3) to slide synchronously.

8. The multi-channel fluorescence detection module according to claim 1, characterized in that: A plurality of first channels (211) corresponding one to one to the plurality of excitation light generating units (22) are linearly arranged on the first slider (21) along the length direction of the first slider (21), and each excitation light generating unit (22) comprises a first lens (221) and a first filter (222), wherein the first lens (221) is arranged at the bottom of the corresponding first channel (211), and the first filter (222) is arranged at the top of the corresponding first channel (211).

9. The multi-channel fluorescence detection module according to claim 2, characterized in that: A plurality of second channels (311) corresponding one to one to the plurality of emission light generating units (32) are linearly arranged on the second slider (31) along the length direction of the second slider (31); each emission light generating unit (32) comprises a second lens (321), a second filter (322) and a reflector (323); the second lens (321) is arranged at the top of the corresponding second channel (311); the second filter (322) is arranged at the bottom of the corresponding second channel (311); and the reflector (323) is arranged between the second lens (321) and the second filter (322).

10. A detector, comprising the multi-channel fluorescence detection module according to any one of claims 1 to 9.

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