Nucleic acid mass spectrometry system

Abstract

The utility model provides a nucleic acid mass spectrometry system. The nucleic acid mass spectrometry system comprises a pretreatment device, a sampling device and a mass spectrometer, the pretreatment device comprises a first moving mechanism used for providing movement in a first direction of a horizontal plane; the second moving mechanism is used for providing movement in the second direction and the vertical direction of the horizontal plane; the MTP plate, the kit and the sample chip are respectively arranged on the first moving mechanism and move in a first direction along with the first moving mechanism; the camera is arranged on the second moving mechanism and vertically moves along with the second moving mechanism to obtain images of the MTP plate and the sample chip; the sampling module is arranged on the second moving mechanism and is used for transferring liquid among the kit, the MTP plate and the chip. The device has the advantages of high moving precision and the like.

Description

Technical Field

[0001] The utility model relates to mass spectrometry analysis, in particular to a nucleic acid mass spectrometry analysis system. Background Art

[0002] In a nucleic acid mass spectrometry detection system, a sample spot is placed on the chip's substrate using a spotting module before testing. Since the diameter of the substrate spot on the chip is only 500 microns, the sample spot must be slightly smaller and centered. This places high demands on the positioning accuracy of the sample needle on the spotting module, necessitating a reliable positioning method. Furthermore, to ensure subsequent analysis, the sample spot's topography and position must be observed and photographed for preservation, necessitating a reliable detection method.

[0003] In the sample spotting module, to improve the accuracy of the sample needle's placement on the chip, a three-axis automated mechanism is typically used to achieve two-dimensional and vertical motion of the sample needle on the chip. The precision of the sample needle's movement depends on the accuracy of the motion mechanism itself. Currently, the most common motion mechanism uses a stepper motor driven by a screw nut or pulley synchronous belt. However, this solution has the following drawbacks:

[0004] 1. There is a gap between the screw nut or the pulley and synchronous belt, and the stepper motor has problems such as loss of step, which cannot meet the high-precision positioning requirements.

[0005] 2. For small dots with a diameter of 500 microns, relying solely on manual observation has problems such as low efficiency, low accuracy, and inability to save images.

[0006] In a nucleic acid mass spectrometry system, a target plate containing a sample must be transferred from atmospheric pressure to the high vacuum environment within the instrument. This transfer must be precise and reliable, and must not damage the high vacuum system within the instrument.

[0007] Most sample target plates use a multi-stage connecting rod lifting mechanism or other mechanical lifting mechanism. The horizontal drive mechanism hits the mechanical lifting mechanism, converting the horizontal motion of the horizontal drive mechanism into vertical reciprocating motion of the lifting mechanism to achieve the subsequent sample target plate loading and unloading function. The shortcomings of these mechanisms are:

[0008] 1. Multi-stage linkage mechanisms or other mechanical lifting mechanisms are usually complex in structure, large in size, difficult to assemble, and prone to failures such as failure to lift the target plate during movement.

[0009] 2. The mechanical lifting mechanism itself has no self-locking function, and the position is easily offset during the transportation of the instrument, causing the target plate platform to fall off during the operation of the instrument, requiring the vacuum system to be destroyed for repair, or even the entire vacuum chamber to be replaced. Summary of the Invention

[0010] In order to solve the deficiencies in the above-mentioned prior art solutions, the present invention provides a nucleic acid mass spectrometry analysis system.

[0011] The purpose of this utility model is achieved through the following technical solutions:

[0012] A nucleic acid mass spectrometry analysis system includes a pretreatment device, a sample injection device, and a mass spectrometer; the pretreatment device includes:

[0013] a first moving mechanism, the first moving mechanism being configured to provide movement in a first direction on a horizontal plane;

[0014] a second movement mechanism, the second movement mechanism being configured to provide movement in a second horizontal direction and in a vertical direction;

[0015] An MTP plate, a reagent kit, and a sample chip, wherein the MTP plate, the reagent kit, and the sample chip are respectively arranged on the first moving mechanism and move in a first direction along with the first moving mechanism;

[0016] The camera and sampling module are arranged on the second moving mechanism and obtain images of the MTP plate and the sample chip as the second moving mechanism moves vertically; the sampling module is arranged on the second moving mechanism and is used to transfer liquid between the reagent kit, the MTP plate and the chip.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. High positioning accuracy;

[0019] A camera is installed on the vertical moving mechanism of the pre-processing device to assist the moving mechanism in reducing the impact of mechanism gap or stepping motor loss of step, improving the spotting positioning accuracy, and ensuring reliable operation and accurate positioning;

[0020] 2. The analysis process is controllable;

[0021] The camera is used to identify the shape and position of the sample points in real time and save the image, making the entire sampling process safe and controllable, improving the operating efficiency of the instrument, and facilitating subsequent analysis;

[0022] 3. The closed space inside the through hole is formed stably;

[0023] The vacuum chamber cover has a through hole, and a closed space is formed in the through hole by using the upper cover and the carrier plate, which realizes normal pressure sampling without affecting the vacuum degree in the vacuum chamber;

[0024] The fourth moving mechanism uses a transmission design of a turbine and worm gear with spiral grooves or holes to convert the rotational motion of the motor into vertical axial motion of the carrier plate. This increases the transmission ratio and has a self-locking function. It can directly transmit atmospheric pressure to the bottom surface of the passage, reducing the torque requirements of the drive mechanism.

[0025] The self-locking function prevents the moving parts in the vacuum chamber from shifting during the transportation of the instrument;

[0026] 4. Simple structure;

[0027] The various components used have simple structures. For example, the fourth moving mechanism reduces the size of the vertical moving mechanism while ensuring reliable operation and accurate positioning. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The disclosure of the present invention will become more easily understood with reference to the accompanying drawings. Those skilled in the art will readily understand that these drawings are merely used to illustrate the technical solution of the present invention and are not intended to limit the scope of protection of the present invention.

[0029] Figure 1 1 is a schematic structural diagram of a pretreatment device according to an embodiment of the present utility model;

[0030] Figure 2 It is a structural schematic diagram of a sample injection device according to an embodiment of the utility model;

[0031] Figure 3 It is a structural schematic diagram of a sample injection device according to an embodiment of the utility model;

[0032] Figure 4 It is a structural schematic diagram of a sample injection device according to an embodiment of the utility model;

[0033] Figure 5 It is a structural schematic diagram of the moving mechanism according to an embodiment of the utility model;

[0034] Figure 6 It is a structural schematic diagram of a carrier plate according to an embodiment of the utility model;

[0035] Figure 7 It is a structural schematic diagram of the fourth moving mechanism according to an embodiment of the present utility model. DETAILED DESCRIPTION

[0036] Figure 1-Figure 7 The following description describes optional specific embodiments of the present invention to teach those skilled in the art how to implement and reproduce the present invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art should understand that variations or replacements derived from these specific embodiments will be within the scope of the present invention. Those skilled in the art should understand that the following features can be combined in various ways to form multiple variations of the present invention. Therefore, the present invention is not limited to the following optional specific embodiments, but is only limited by the claims and their equivalents.

[0037] Example 1

[0038] The nucleic acid mass spectrometry analysis system of the present invention includes a pretreatment device, a sample injection device and a mass spectrometer, such as Figure 1 As shown, the pre-processing device includes:

[0039] A first moving mechanism 51, the first moving mechanism 51 is used to provide movement in a first direction on a horizontal plane;

[0040] A second moving mechanism 52, the second moving mechanism 52 is used to provide movement in a second horizontal direction and a vertical direction;

[0041] An MTP plate 72, a reagent kit 71, and a sample chip 73, wherein the MTP plate 72, the reagent kit 71, and the sample chip 73 are respectively arranged on the first moving mechanism 51 and move in the first direction along with the first moving mechanism 51;

[0042] The camera 61 and the sampling module are arranged on the second movable mechanism 53, and obtain images of the MTP plate 72 and the sample chip 73 as the second movable mechanism 52 moves vertically; the sampling module is arranged on the second movable mechanism 52, and is used to transfer liquid between the reagent kit 71, the MTP plate 72 and the sample chip 73.

[0043] In order to automatically transfer the sample chip, the nucleic acid mass spectrometry analysis system further includes:

[0044] A robot arm is used to transfer the sample chip 73, such as from the pre-processing device to the sample injection device.

[0045] In order to realize automatic injection, Figure 2-3 As shown, the sampling device includes:

[0046] A vacuum chamber 11 and an upper cover 12, wherein the vacuum chamber 11 has a cover plate 12, the cover plate 12 has a through hole 13, and the upper cover is used to close the outer opening of the through hole 13;

[0047] a pressure adjustment unit 31 , the pressure adjustment unit 31 being connected to the through hole 13 and being used to adjust the gas pressure in the through hole 13 ;

[0048] Load plates and mobile platforms, such as Figure 4-5 As shown, the supporting plate 23 is used to carry samples and is arranged in the vacuum chamber 11; the movable platform is arranged in the vacuum chamber 11, including a third moving mechanism 21 that provides two-dimensional movement on the horizontal plane and a fourth moving mechanism 22 that provides vertical movement. The fourth moving mechanism 22 drives the supporting plate 23 to move vertically to close or open the inner opening of the through hole 13.

[0049] In order to reduce the load on the third moving mechanism, further, as Figure 4 As shown, the fourth moving mechanism 22 is fixed on the bottom plate of the vacuum chamber 11 and is located at the lower side of the through hole 13;

[0050] like Figure 5 As shown, the carrying plate 23 is arranged on the third moving mechanism 21 .

[0051] In order to facilitate connection and separation, further, as Figure 5 As shown, the carrying plate 23 is set on the third moving mechanism 21 through a magnetic module. When the fourth moving mechanism 22 drives the carrying plate 23 to move upward, the carrying plate 23 is separated from the third moving mechanism 21.

[0052] In order to facilitate and accurately locate, further, such as Figure 6 As shown, multiple columns 211 are set on the third moving mechanism 21, and the bottom side of the supporting plate 23 has a groove 231 allowing the top of the column to enter, and the magnetic attraction module is respectively set on the top of the column 211 and in the groove 231.

[0053] In order to accurately push the carrying plate 23 to move vertically, further, as Figure 7 As shown, the fourth moving mechanism 22 includes:

[0054] A worm 223 and a power source 220 , wherein the power source 220 drives the worm 223 to rotate;

[0055] A worm gear 224 and a rotating shaft 222 , wherein the worm gear 224 is fixed to the outside of the rotating shaft 222 and engages with the worm 223 ;

[0056] A rotating arm 226 and a moving member 225, wherein the rotating arm 226 is fixed to the rotating shaft 222, and the moving member 225 is arranged on the outer side of the rotating shaft 222 and has a spiral groove or through hole allowing the rotating arm 226 to extend into;

[0057] A guide member 229 and a base 221 , wherein the guide member 229 is fixed on the base 221 and passes through the moving member; the power source 220 , the worm 223 and the rotating shaft 222 are arranged on the base 221 .

[0058] In order to adjust the pressure in the through hole 13 so that the through hole 13 is connected to the outside or the vacuum chamber 11, further, as shown in FIG. Figure 3-4 As shown, the pressure adjustment unit 31 includes:

[0059] An air pump 311 and a switching module 312, wherein the switching module 312 is used to selectively connect the second channel to the atmosphere or the first channel, and the air pump 311 is connected to the first channel;

[0060] A second channel is provided in the cover plate 12 and communicates with the through hole 13 .

[0061] The working mode of the nucleic acid mass spectrometry analysis system of the embodiment of the present invention includes a pretreatment stage and a sample injection stage. The pretreatment stage is:

[0062] The sample is placed on the MTP plate 72, the reagent kit 71 is filled with resin, and the MTP plate 71, the reagent kit 71 and the sample chip 73 are all arranged at different positions on the first moving mechanism 51;

[0063] The movement of the first and second moving mechanisms 51 and 52 allows the sampling module to extract resin from the reagent kit 71, then transfer the extracted resin to the sample on the MTP plate 72. Finally, the liquid in the MTP plate 72 is extracted and transferred to the sample chip 73. During this process, the camera 61 identifies the exact positions of the reagent kit 71, MTP plate 72, and sample chip 73, as well as the presence of resin, sample, and the topography and position of the sample points on the sample chip 73.

[0064] After the pretreatment is completed, the robot grabs the sample chip 73 and transfers it to the sample injection device.

[0065] The injection stage is:

[0066] The upper cover closes the outer opening of the through hole 13, and the vacuum chamber 11 maintains a vacuum state;

[0067] In the vacuum chamber 11 , the third moving mechanism 21 drives the carrying plate 23 to move two-dimensionally in the horizontal plane until the carrying plate 23 is located below the through hole 13 ;

[0068] The fourth moving mechanism 22 drives the carrying plate 23 to move upward, closing the inner opening of the through hole 13, and the through hole 13 becomes a closed space;

[0069] The pressure in the through hole 13 is increased, the upper cover is opened, the through hole 13 is connected to the outside, and the robot places the sample chip grabbed on the carrier plate 23 in the through hole 13;

[0070] The upper cover closes the outer opening, and the through hole 13 becomes a closed space, and the gas in the through hole 13 is extracted, so that the pressure in the through hole 13 decreases;

[0071] The fourth moving mechanism 22 moves downward to open the inner opening, and the through hole 13 is connected to the interior of the vacuum chamber 11 .

[0072] In order to reduce the load of the third moving mechanism 21, the third moving mechanism 21 further moves the carrying plate 23 to the upper side of the fourth moving mechanism 22, and the fourth moving mechanism 22 drives the carrying plate 23 to separate from the third moving mechanism 21 and move upward;

[0073] The fourth moving mechanism 22 drives the carrying plate 23 to move downward, and the carrying plate 23 is carried by the third moving mechanism 21 , and the fourth moving mechanism 22 continues to move downward.

[0074] In order to accurately move the carrying plate, further, the driving mode of the fourth moving mechanism is:

[0075] The worm 223 rotates, driving the worm wheel 224 to rotate, causing the rotating shaft 222 and the rotating arm 226 to rotate;

[0076] The rotating arm 226 rotates in the spiral groove or hole of the moving member 225, driving the moving member 225 to move up or down.

[0077] In order to adjust the pressure in the through hole 13 so that the through hole 13 is connected to the outside or the vacuum chamber 11, the pressure in the through hole 13 is further adjusted as follows:

[0078] The switching module 312 switches so that the second channel is connected to the outside and the second channel is connected to the through hole 13, thereby increasing the pressure in the through hole 13. The second channel is provided on the cover plate 12.

[0079] The switching module 312 is switched, and the second channel is connected to the air pump 311 . The air pump 311 extracts the gas in the through hole 12 , thereby reducing the pressure in the through hole 13 .

[0080] Example 2

[0081] An application example of a nucleic acid mass spectrometry analysis system according to Example 1 of the present utility model.

[0082] In this application example, Figure 1 As shown, the first movable mechanism 51 and the second movable mechanism 52 are separate and both utilize conventional technologies, such as guide rails, motors, and screws. The first movable mechanism 51 is fixed to the bracket 41 and is used to provide movement in a first horizontal direction, while the second movable mechanism 52 is used to provide movement in a second horizontal direction and in the vertical direction. A camera 61 is mounted on the second movable mechanism 53 and, as the second movable mechanism 52 moves vertically, acquires images of the reagent kit 71, MTP plate 72, and sample chip 73. The sampling module is mounted on the second movable mechanism 52 and is used to transfer liquid between the reagent kit 71, MTP plate 72, and sample chip 73.

[0083] A robot arm is used to transfer the sample chip 73, such as from the pre-processing device to the sample injection device.

[0084] like Figure 2-3 As shown, in the sample injection device, a cover plate 12 is provided on the upper side of the vacuum chamber 11. A through hole 13 is provided on the cover plate 12. A second channel is provided within the cover plate 12 and connects to the through hole 13. Seals, such as O-rings, are provided at the outer and inner openings of the through hole 13. The upper cover seals the outer opening, and the carrier plate 23 is adapted to seal the inner opening, creating a closed space within the through hole 13.

[0085] like Figure 3-4 As shown, the switching module 312 is a two-position, three-way solenoid valve fixed to the vacuum chamber 11. The vacuum pump 311 is fixed to the vacuum chamber 311 and connected to the switching module 312 via a first channel (pipe). The outer outlet of the second channel is connected to the switching module 312, allowing the through hole 13 to selectively connect the outside world and the first channel.

[0086] like Figure 5 As shown, the third moving mechanism 21 adopts a two-dimensional moving platform, such as guide rails, screw rods and slides, to provide two-dimensional movement in the horizontal plane. The supporting plate 23 is arranged on the third moving mechanism 21, and has a groove 232 on the upper side for accommodating the sample.

[0087] like Figure 6 As shown, four columns 211 are set on the third moving mechanism 21, and correspondingly, the bottom side of the supporting plate 23 has four grooves 231, and the magnetic modules are respectively arranged at the top of the columns 211 and in the grooves 231, so that the supporting plate 23 can be conveniently and accurately connected to and detached from the third moving mechanism 21.

[0088] like Figure 7 As shown, the fourth moving mechanism 21 is disposed within the vacuum chamber 11 and below the through-hole 13. The power source 220 is a motor, and a screw 223 is disposed on the base 221. The motor is connected to the screw 223 via a coupling. A worm gear 224, which engages with the screw 223, is fixed to the outside of the rotating shaft 222. The rotating arm 226 is a pin, also fixed to the outside of the rotating shaft 222. The bottom end of the rotating arm 222 is mounted on the base 221 via a bearing 227. The moving member 225 is cylindrical, with a spiral through-hole 2261 defined in its sidewall. The pin is located within the spiral through-hole 2261, so that when the screw rotates, the pin pushes the moving member 225 upward or downward. The upper side of the moving member 225 is connected to the supporting member 228. Multiple guide members 229 (guide rods in this embodiment) are fixed to the base 221 and pass through the supporting member 228, allowing the supporting member 228 to move only vertically up and down.

[0089] The working mode of the nucleic acid mass spectrometry analysis system of this embodiment includes a pretreatment stage and a sample injection stage. The pretreatment stage is:

[0090] The sample is placed on the MTP plate 72, the reagent kit 71 is filled with resin, and the MTP plate 71, the reagent kit 71 and the sample chip 73 are all arranged at different positions on the first moving mechanism 51;

[0091] The movement of the first and second moving mechanisms 51 and 52 allows the sampling module to extract resin from the reagent kit 71, then transfer the extracted resin to the sample on the MTP plate 72. Finally, the liquid in the MTP plate 72 is extracted and transferred to the sample chip 73. During this process, the camera 61 identifies the exact positions of the reagent kit 71, MTP plate 72, and sample chip 73, as well as the presence of resin, sample, and the topography and position of the sample points on the sample chip 73.

[0092] After the pretreatment is completed, the robot grabs the sample chip 73 and transfers it to the sample injection device.

[0093] The injection stage is:

[0094] The upper cover closes the outer opening of the through hole 13, and the vacuum chamber 11 maintains a vacuum state;

[0095] In the vacuum chamber 11 , the third moving mechanism 21 drives the carrying plate 23 to move two-dimensionally in the horizontal plane until the carrying plate 23 is located below the through hole 13 and above the fourth moving mechanism 22 .

[0096] The worm 223 rotates forward, driving the worm wheel 224 to rotate, causing the rotating shaft 222 and the rotating arm 226 to rotate; the rotating rotating arm 226 rotates in the spiral hole of the moving member 225, driving the moving member 225 and the supporting member 228 to move upward, and the supporting member 228 moves upward against the supporting plate 23, separating the supporting plate 23 and the column 211, and the upwardly moved supporting plate 23 closes the inner opening of the through hole 13, and the through hole 13 becomes a closed space;

[0097] The switching module 312 switches, so that the through hole 13 is connected to the outside world through the second channel, the pressure in the through hole 13 is increased, the upper cover is opened, and the through hole 13 is connected to the outside world. The manipulator places the sample chip on the carrier plate 23 in the through hole 13;

[0098] The upper cover closes the outer opening, and the through hole 13 becomes a closed space. The switching module 312 switches so that the second channel is connected to the first channel, and the air pump 311 extracts the gas in the through hole 13, so that the pressure in the through hole 13 drops to the pressure in the vacuum chamber 11.

[0099] The worm 223 rotates in the opposite direction, driving the worm wheel 224 to rotate, causing the rotating shaft 222 and the rotating arm 226 to rotate; the rotating rotating arm 226 rotates in the spiral through hole 2261 of the moving member 225, driving the moving member 225 and the supporting member 228 to move downward, and the supporting member 228 carries the supporting plate 23 and moves downward, thereby opening the inner opening, and the through hole 13 is connected to the interior of the vacuum chamber 11;

[0100] The sample is ionized and then sent to a mass spectrometer for analysis;

[0101] In the vacuum chamber 11 , the third moving mechanism 21 drives the carrying plate 23 to move two-dimensionally in the horizontal plane until the carrying plate 23 is located below the through hole 13 and above the fourth moving mechanism 22 .

[0102] The worm 223 rotates forward, driving the worm wheel 224 to rotate, causing the rotating shaft 222 and the rotating arm 226 to rotate; the rotating rotating arm 226 rotates in the spiral hole of the moving member 225, driving the moving member 225 and the supporting member 228 to move upward, and the supporting member 228 moves upward against the supporting plate 23, separating the supporting plate 23 and the column 211, and the upwardly moved supporting plate 23 closes the inner opening of the through hole 13, and the through hole 13 becomes a closed space;

[0103] The switching module 312 switches so that the through hole 13 is connected to the outside world through the second channel, the pressure in the through hole 13 is increased, the upper cover is opened, the through hole 13 is connected to the outside world, and the robot grabs the tested sample chip 73 and places it in the recovery area.

[0104] Example 3

[0105] The application example of the nucleic acid mass spectrometry analysis system according to Example 1 of the present invention is different from that of Example 2 in that:

[0106] 1. No need to set the column 211, magnetic module and groove 231;

[0107] The supporting plate 23 is fixed on the upper side of the supporting member 228 , and the fourth moving mechanism 22 is disposed on the third moving mechanism 21 .

[0108] 2. During operation, the third moving mechanism 21 moves the carrying plate 23 to the lower side of the through hole 13, and then the fourth moving mechanism 22 drives the carrying plate 23 upward, thereby sealing the inner opening of the through hole 13;

[0109] The fourth moving mechanism 22 drives the carrying plate 23 to move downward, thereby connecting the through hole 13 with the interior of the vacuum chamber 11 .

Claims

1. A nucleic acid mass spectrometry analysis system, comprising a pretreatment device, a sample injection device and a mass spectrometer; It is characterized in that The pre-processing device comprises: a first moving mechanism, the first moving mechanism being used to provide movement in a first direction on a horizontal plane; A second moving mechanism, the second moving mechanism is used to provide movement in a second direction of the horizontal plane and in a vertical direction; An MTP plate, a reagent kit and a sample chip, wherein the MTP plate, the reagent kit and the sample chip are respectively arranged on the first moving mechanism and move in a first direction along with the first moving mechanism; A camera and a sampling module, wherein the camera is arranged on the second moving mechanism and obtains images of the MTP plate and the sample chip as the second moving mechanism moves vertically; the sampling module is arranged on the second moving mechanism and is used to transfer liquid between the reagent kit, the MTP plate and the chip.

2. The nucleic acid mass spectrometry analysis system according to claim 1, It is characterized in that The nucleic acid mass spectrometry analysis system further comprises: A robot arm is used to transfer the sample chip.

3. The nucleic acid mass spectrometry analysis system according to claim 2, It is characterized in that The sample injection device comprises: A vacuum chamber and an upper cover, wherein the vacuum chamber has a cover plate, the cover plate has a through hole, and the upper cover is used to close the outer opening of the through hole; a pressure adjustment unit, the pressure adjustment unit being connected to the through hole and used for adjusting the gas pressure in the through hole; A carrying plate and a moving platform, wherein the carrying plate is used to carry the sample chip and is arranged in the vacuum chamber; the moving platform is arranged in the vacuum chamber, and includes a third moving mechanism that provides two-dimensional movement in a horizontal plane and a fourth moving mechanism that provides vertical movement, wherein the fourth moving mechanism drives the carrying plate to move vertically to close or open the inner opening of the through hole; the manipulator is used to transfer the sample chip in the pretreatment device to the carrying plate in a state where the inner opening is closed.

4. The nucleic acid mass spectrometry analysis system according to claim 3, It is characterized in that The carrying plate is arranged on the third moving mechanism, and the fourth moving mechanism is fixed on the bottom plate of the vacuum chamber and is located at the lower side of the through hole.

5. The nucleic acid mass spectrometry analysis system according to claim 4, It is characterized in that The supporting plate is arranged on the third moving mechanism through a magnetic module; a plurality of columns are arranged on the third moving mechanism, the bottom side of the supporting plate has a groove allowing the top of the column to enter, and the magnetic module is respectively arranged on the top of the column and in the groove.

6. The nucleic acid mass spectrometry analysis system according to claim 3, It is characterized in that The fourth moving mechanism comprises: A worm and a power source, wherein the power source drives the worm to rotate; A worm wheel and a rotating shaft, wherein the worm wheel is fixed to the outside of the rotating shaft and meshes with the worm; A rotating arm and a moving member, wherein the rotating arm is fixed on the rotating shaft, and the moving member is arranged on the outer side of the rotating shaft and has a spiral groove or a through hole allowing the rotating arm to extend therein; A guide member and a base, wherein the guide member is fixed on the base and passes through the moving member; the power source, the worm and the rotating shaft are arranged on the base.

7. The nucleic acid mass spectrometry analysis system according to claim 3, It is characterized in that The pressure adjustment unit comprises: An air extraction pump and a switching module, wherein the switching module is used to selectively connect the second channel to the atmosphere or the first channel, and the air extraction pump is connected to the first channel; A second channel is disposed in the cover plate and communicated with the through hole.

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