Nasal secretion sample collecting and storing device

Abstract

The utility model discloses a nasal secretion sample collection and preservation device, which comprises a collection component and a preservation component, the collection component is composed of a handheld part and a secretion adsorption part, the handheld part is made of common PVC (polyvinyl chloride) plastics, the secretion adsorption part is arranged at the tail end of the handheld part, and the preservation component is arranged on the handheld part. An adsorption material with the water absorption rate of 100-500 [mu] l / cm < 3 > and the release rate of 100-400 [mu] l / cm < 3 > is made into a cylindrical structure or a rod-shaped structure; the retention assembly is composed of an extrusion tube and a collection tube, the extrusion tube is a needle tube made of a common PVC plastic material, the inner diameter of the extrusion tube is tightly attached to the outer diameter of the collection assembly without air leakage, and the collection tube is a glass tube containing a protein protective agent. When the nasal secretion collector is used for collecting nasal secretion, only physical extrusion is needed, centrifugation and other means are not needed, the nasal secretion can be simply, conveniently and rapidly collected, and instruments such as a centrifugal machine are not needed.

Description

Technical Field

[0001] The utility model relates to the field of medical instruments for collecting human secretions, and more specifically, to a nasal secretion sample collecting and retaining device suitable for local nasal detection. Background Art

[0002] The diagnosis of allergic rhinitis mainly relies on the patient's clinical symptoms and signs, medical history and allergen test results. There are currently two main detection methods: one is in vivo testing: including intradermal testing, prick testing, scratch testing, patch testing, conjunctival provocation testing, nasal mucosal provocation testing and bronchial provocation testing; the other is in vitro testing: by extracting a small amount of serum, testing the content of specific IgE produced in the serum to certain allergens, or testing other cytokines (IL-4 / γ-IFN).

[0003] However, the above detection methods still have great defects: 1. Whether it is an in vivo test or an in vitro test, there is physical trauma, and the patient compliance is poor; 2. It reflects systemic symptoms and ignores the local nasal inflammation. For example, the skin prick test is easily affected by oral medications, and systemic diseases can also interfere with the detection of specific IgE. For example, patients with negative serum-specific IgE test results are positive when nasal secretions are tested. The determination of nasal secretions can not only reflect the local inflammatory state of the patient's nasal cavity, but more importantly, the determination of nasal secretions is more non-invasive than the determination of serum. It is a detection method with high patient acceptance and good cooperation. Accurately assessing the local nasal inflammation state of patients in a non-invasive way has become the first step to be solved in rhinitis detection.

[0004] At present, the tools and materials used to collect nasal secretions vary, generally simple medical gauze, handmade sponges (expanded sponges) or aspirators (negative pressure); there are also differences in the processing methods, such as the use of physiological saline perfusion and then collection, the use of different sample preservation solutions, different processing methods (such as nucleic acid extraction and purification, antigen repair conditions, etc.), and the lack of strict standardized devices and methods. The result is that the test results are greatly affected by the sampling devices and methods, not only quantitative problems, but also qualitatively different. For example, when a sponge that is too large is inserted into the nasal cavity, it will stimulate the nose to secrete a large amount of catarrhal fluid, and ultimately dilute the test object (the content of some cytokines is expressed in a low abundance in the secretions). Therefore, the standardized acquisition of nasal secretion samples suitable for local nasal testing has become the second problem to be solved in rhinitis detection.

[0005] However, since the content of some proteins to be tested in nasal secretions is very small, and the secretions contain tissue fluid, mucin, lysozyme, and various products of tissue cell lysis, including proteases and nucleases, which can decompose some proteins and nucleic acids. Therefore, the stability of secretions is not as good as that of serum. For example, eosinophil cationic protein (ECP) and myeloperoxidase (MPO) in nasal secretions will degrade over time in nasal secretions. At room temperature, more than 50% will be degraded after one week. Therefore, the preservation of nasal secretions becomes the third problem to be solved in rhinitis detection. Summary of the invention

[0006] In view of the deficiencies in the prior art, the utility model provides a simple, easy-to-operate, non-invasive nasal secretion sample collection and retention device suitable for local nasal detection, which can realize simple and quick collection of nasal secretions.

[0007] To achieve the above purpose, the technical solution adopted by the utility model is: a nasal secretion sample collection and retention device, including a collection component and a retention component, the collection component is composed of a hand grip and a secretion adsorbent, wherein the hand grip is made of ordinary PVC plastic material, the secretion adsorbent is arranged at the end of the hand grip, and the water absorption rate is 100-500ul / cm 3 , release rate is 100-400ul / cm 3 The adsorption material is made into a columnar structure or a rod-shaped structure; the retention component consists of an extrusion tube and a collection tube, wherein the extrusion tube is a needle tube made of ordinary PVC plastic material, the inner diameter of the extrusion tube is tightly fitted with the outer diameter of the collection component without air leakage, and the collection tube is a glass tube containing a protein protective agent.

[0008] When the utility model is used, the following steps are included:

[0009] (a) Hold the hand grip and insert the secretion absorber directly into the middle nasal meatus, and remove the secretion absorber after 5 minutes;

[0010] (b) inserting a secretion absorbent having a nasal secretion sample collected therein into one end of the extrusion tube, and inserting the other end of the extrusion tube into the collection tube;

[0011] (c) The nasal secretion liquid adsorbed in the secretion adsorbent is squeezed into the collection tube by mechanical force and mixed evenly, and the operation is completed.

[0012] Compared with the prior art, the utility model only needs physical squeezing when collecting nasal secretions, without the need for centrifugation or other means, thereby realizing a simple and quick collection of nasal secretions without the need for instruments such as centrifuges. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of the acquisition component in the utility model;

[0014] Figure 2 It is a structural schematic diagram of the retained components in the utility model.

[0015] In the figure: 1-collection component; 11-hand-held component; 12-secretion adsorption component; 2-retention component; 21-extrusion tube; 22-collection tube. DETAILED DESCRIPTION

[0016] The embodiments of the present invention will be described in detail below in conjunction with the embodiments in the accompanying drawings, but those skilled in the art will understand that the following embodiments are only used to illustrate the utility model and should not be considered to limit the scope of the present invention. If no specific conditions are specified in the embodiments, they are carried out under conventional conditions or conditions recommended by the manufacturer. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present utility model.

[0017] like Figure 1 and Figure 2 As shown, the utility model provides a nasal secretion sample collection and retention device, which includes a collection component 1 and a retention component 2. The collection component 1 is composed of a hand-held component 11 and a secretion adsorbent 12, wherein the hand-held component 11 is made of ordinary PVC plastic material, and the secretion adsorbent 12 is arranged at the end of the hand-held component 11. The secretion adsorbent 12 adopts a water absorption rate of 100-500ul / cm 3 , release rate is 100-400ul / cm 3 The adsorbent material is made into a columnar structure or a rod-shaped structure, and the secretion adsorbent 12 has a length, width and height of (0.1cm-1.0cm)*(0.1cm-1.0cm)*(0.1cm-1.0cm), preferably a length, width and height of 0.5cm*0.5cm*0.5cm, and a water absorption rate of 500ul / cm 3 , release rate is 400ul / cm 3 .

[0018] The adsorbent material of the secretion adsorbent 12 is selected from one or more of nitrocellulose membrane material, glass cellulose membrane material, polysulfone membrane material, nylon membrane material, polyester fiber, and filter paper, preferably polyester fiber.

[0019] The advantages of selecting the above-mentioned adsorption material are: 1. The water absorption rate of the material can ensure the quantitative adsorption of a certain amount of nasal secretions; 2. The release rate of the material can ensure the quantitative release of cytokines in the nasal secretions; 3. The human compatibility of the material ensures that it will not irritate the local part of the nose, secrete a large amount of catarrhal fluid, and ultimately dilute the object to be detected.

[0020] The retention component 2 is composed of an extrusion tube 21 and a collection tube 22, wherein the extrusion tube 21 is a needle tube made of ordinary PVC plastic material, and the inner diameter of the extrusion tube 21 fits tightly with the outer diameter of the collection component 1 without leakage, so as to facilitate the squeezing out of the nasal secretions collected by the secretion adsorbent 12; the collection tube 22 is a glass tube containing a protein protective agent, and the glass tube is selected from one or more of medium-borosilicate ampoules, low-borosilicate ampoules, neutral glass tubes, soda-lime glass tubes, polyethylene (PE) centrifuge tubes, polycarbonate (PC) centrifuge tubes, polypropylene (PP) centrifuge tubes, and polytetrafluoroethylene centrifuge tubes, preferably medium-borosilicate ampoules.

[0021] The advantages of choosing medium-borosilicate ampoules are: the surface water resistance of medium-borosilicate ampoules is not less than the national standard of 2.6mm, the less harmful alkaline oxides such as potassium and sodium adsorbed on the inner wall of the vial, the better the water resistance, and good water resistance has very good effects on various indicators such as the pH, acidity and alkalinity of the solution, clarity, stability, etc., and the amount of protein adsorption is small, and the adsorption of trace proteins by the material will not ultimately affect the test results.

[0022] When the utility model is used, the following steps are included:

[0023] (a) holding the hand grip 11, inserting the secretion absorber 12 directly into the middle nasal meatus, and removing the secretion absorber 12 after 5 minutes;

[0024] (b) inserting the secretion adsorbent 12 containing the nasal secretion sample into one end of the extrusion tube 21, and inserting the other end of the extrusion tube 21 into the collection tube 22;

[0025] (c) The nasal secretion liquid adsorbed in the secretion adsorbent 12 is squeezed into the collection tube 22 by mechanical force and mixed evenly, and the operation is completed.

[0026] When collecting nasal secretions, the utility model only needs physical squeezing instead of centrifugation and the like, thereby realizing simple and quick collection of nasal secretions without the aid of instruments such as centrifuges.

Claims

1. A nasal secretion sample collection and retention device, characterized in that: It includes a collection component and a retention component. The collection component consists of a hand grip and a secretion adsorbent. The hand grip is made of ordinary PVC plastic material. The secretion adsorbent is set at the end of the hand grip and has a water absorption rate of 100-500ul / cm 3 , release rate is 100-400ul / cm 3 The adsorption material is made into a columnar structure or a rod-shaped structure; the retention component consists of an extrusion tube and a collection tube, wherein the extrusion tube is a needle tube made of ordinary PVC plastic material, the inner diameter of the extrusion tube is tightly fitted with the outer diameter of the collection component without air leakage, and the collection tube is a glass tube containing a protein protective agent.

2. The nasal secretion sample collection and storage device according to claim 1, characterized in that: The length, width and height of the secretion absorber are (0.1 cm-1.0 cm)*(0.1 cm-1.0 cm)*(0.1 cm-1.0 cm).

3. The nasal secretion sample collection and retention device according to claim 2, characterized in that: The secretion adsorbent has a length, width and height of 0.5 cm*0.5 cm*0.5 cm, and a water absorption rate of 500 ul / cm 3 , release rate is 400ul / cm 3 .

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