Temperature control magnetic suction module and sequencing hybridization capture library construction automation work station
By designing a temperature-controlled magnetic suction module, combining the heating plate and magnetic suction unit, the problem that the sample liquid temperature cannot be kept constant in the existing technology is solved, and the constant temperature control and automation process in the construction of tumor hybrid capture library is realized, and the sequencing is improved Data quality and library building success rate.
Patent Information
- Application Number
- CN202421379364.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-17
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2034-06-17
AI Technical Summary
In the existing tumor hybrid capture library construction equipment, the temperature control module and the magnetic bead magnetic washing hybrid module exist independently, resulting in the sample liquid temperature not being kept constant, resulting in the large content of some base fragments of non-specific hybridization, affecting the quality of sequencing data.
A temperature-controlled magnetic suction module is designed, including a heating plate, a thermally conductive PCR board carrier and a magnetic suction unit, which can maintain the constant temperature of the sample liquid during the washing and hybridization process, and achieve effective hybridization capture through the adsorption and lifting of magnetic beads. , reduce the removal of nonspecific gene fragments.
The constant temperature control of the sample solution during the washing and hybridization process is realized, the quality of sequencing data is improved, and the probability of library construction failure caused by manual operations is reduced through automated processes, and the problem of library differences between batches is solved.
Smart Images
Figure CN222226394U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of biological sample processing, and specifically relates to a temperature-controlled magnetic suction module and a sequencing hybridization capture library construction automated workstation. Background Art
[0002] With the continuous development of biotechnology, the application of tumor gene detection is becoming more and more extensive. In the past, the washing and hybridization operations in the process of tumor library construction were all completed manually. However, due to the continuous increase in market demand, the amount of samples tested has also increased, and the demand for high-throughput automation has become more and more urgent. At present, the library construction equipment on the market generally uses ordinary small fragment library construction (excluding hybridization capture). However, in the current equipment for tumor hybridization capture library construction, the temperature control module and the magnetic bead magnetic absorption washing and hybridization module are independent of each other. After the operation on the temperature control module is completed, the sample liquid temperature cannot be maintained at the set temperature when it is transferred to the magnetic bead magnetic absorption washing and hybridization module for washing and hybridization operation. This results in a large content of some base fragment sequences in the sample liquid that are non-specifically hybridized, resulting in lower quality of subsequent sequencing data. Utility Model Content
[0003] The utility model provides a temperature-controlled magnetic absorption module and an automated workstation for constructing a sequencing hybridization capture library, which can solve the technical problem that the temperature control module and the magnetic bead magnetic absorption washing hybridization module in the prior art exist independently, and in the process of transferring the sample solution to the magnetic bead magnetic absorption washing hybridization module for washing and hybridization operation after the operation on the temperature control module is completed, the sample solution temperature cannot be maintained at the set temperature, resulting in a large content of some base fragments of non-specific hybridization in the sample solution, thereby resulting in low quality of subsequent sequencing data.
[0004] In order to solve the above problems, the utility model provides a temperature-controlled magnetic module, including a bracket, a heating plate is provided on the top of the bracket, a PCR plate carrier capable of heat conduction is provided on the top surface of the heating plate, the PCR plate carrier has bearing holes that match the number and shape of the holes in the reaction container, and a magnetic unit is also provided on the bracket, the magnetic unit includes a magnetic column card plate, the magnetic column card plate can be driven to rise and fall, and a plurality of magnetic columns arranged in a square array are provided on the top surface of the magnetic column card plate, and any one of the magnetic columns can adsorb the magnetic beads in four adjacent holes of the reaction container on the side walls of the holes.
[0005] In some embodiments, a heat insulating layer is disposed on the bottom surface of the heating plate; and / or the PCR plate carrier is made of aluminum.
[0006] In some embodiments, a magnetic column receiving groove is formed on the lower end surface of the PCR plate carrier, and each of the magnetic columns can extend into the magnetic column receiving groove when being driven to rise.
[0007] In some embodiments, a heat-insulating layer is applied on the peripheral side walls of the PCR plate carrier.
[0008] In some embodiments, an air groove is formed in the area between four adjacent carrying holes of the PCR plate carrier.
[0009] In some embodiments, the temperature-controlled magnetic suction module further includes a lifting drive component, wherein the lifting drive component has a lifting column, and the lifting column is used to drive the magnetic column card plate to lift and lower.
[0010] In some embodiments, a buffer plate is provided below the magnetic column clamp, a gap is provided between the top surface of the buffer plate and the bottom surface of the magnetic column clamp, and a plurality of elastic members are arranged at intervals in the gap, and the free end of the lifting column is connected to the bottom surface of the buffer plate.
[0011] In some embodiments, the elastic member is a metal coil spring, the upper and lower ends of the metal coil spring are fixedly connected to the magnetic column clamp and the buffer plate respectively, and a plurality of the elastic members are respectively arranged at the four corner areas of the magnetic column clamp.
[0012] In some embodiments, the left and right ends of the buffer plate are slidably connected to the bracket via slide rails respectively.
[0013] The utility model also provides an automated workstation for constructing a sequencing hybridization capture library, comprising the temperature-controlled magnetic suction module mentioned above.
[0014] The utility model provides a temperature-controlled magnetic suction module and an automated workstation for constructing a sequencing hybridization capture library, which has the following beneficial effects:
[0015] The temperature-controlled magnetic module also has a magnetic unit that effectively absorbs the magnetic beads in each hole of the reaction container and a heating plate that can ensure that the reaction container is in a constant temperature control state, so that during the washing and hybridization process, the sample (i.e., the sample liquid) is kept at a constant temperature, and the magnetic attraction is better used to remove non-specific gene fragments, so as to obtain more target fragments and improve the quality of sequencing data. In addition, the temperature-controlled magnetic module of the utility model is also conducive to realizing the automation of the hybridization capture library construction process, reducing the probability of library construction failure caused by manual operation, and solving the problem of large differences in libraries between batches due to manual operation. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the implementation or the prior art description. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.
[0017] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.
[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of the temperature control magnetic suction module of an embodiment of the utility model;
[0019] Figure 2 for Figure 1 A schematic diagram of the three-dimensional structure of the temperature-controlled magnetic absorption module at another angle, showing the state where the magnetic column does not absorb the magnetic beads in the reaction container;
[0020] Figure 3 for Figure 1 A front view of the temperature-controlled magnetic module in FIG. 1 , showing a state in which the magnetic column absorbs the magnetic beads in the reaction container onto the side wall of the hole of the reaction container;
[0021] Figure 4 for Figure 1 A top view of the temperature-controlled magnetic module.
[0022] The reference numerals are as follows:
[0023] 91. Bracket; 921. Heating plate; 922. PCR plate carrier; 9221. Loading hole; 9223. Air slot; 9224. Carrier bottom plate; 923. Heat insulation layer; 924. Insulation layer; 93. Magnetic unit; 931. Magnetic column clamping plate; 9311. Magnetic column; 932. Buffer plate; 933. Elastic part; 934. Magnetic column sealing plate; 94. Lifting drive assembly; 941. Lifting column; 942. Drive component mounting bracket; 95. Slide rail; 100. Reaction vessel. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means a limitation on the utility model and its application or use. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0025] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.
[0026] It should be understood that the term "and / or" used in this article is only a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.
[0027] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values of the parts and steps described in these embodiments do not limit the scope of the utility model. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once a certain item is defined in one drawing, it does not need to be further discussed in subsequent drawings.
[0028] In the description of the present utility model, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction, and therefore cannot be understood as limiting the scope of protection of the present utility model; the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.
[0029] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.
[0030] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the utility model.
[0031] According to the embodiments of the present utility model, specifically in combination with Figures 1 to 4 As shown, a temperature-controlled magnetic module is provided, including a bracket 91, a heating plate 921 is provided on the top of the bracket 91, and a PCR plate carrier 922 capable of heat conduction is provided on the top surface of the heating plate 921. The aforementioned heating plate 921 is, for example, a plate body with an electric heating structure such as a resistance wire and an electric heating film laid inside, and a corresponding temperature sensor can be specifically provided on it to detect its real-time heating temperature to ensure that it can achieve the purpose of constant temperature heating (how to achieve its constant temperature control can be achieved by conventional means in the industry). In a specific embodiment, the aforementioned heating plate 921 is configured to have a heating capacity capable of heating from room temperature to 70°C, and the PCR plate carrier 922 The support 91 is provided with bearing holes 9221 whose number and shape match those of the holes (i.e., reaction tubes) of the reaction container 100. The support 91 is also provided with a magnetic unit 93, which includes a magnetic column card plate 931. The magnetic column card plate 931 can be driven to rise and fall. The top surface of the magnetic column card plate 931 has a plurality of magnetic columns 9311 arranged in a square array. Any of the magnetic columns 9311 can adsorb the magnetic beads in the four adjacent holes of the reaction container 100 onto the side walls of the holes to prevent the automatic pipette from taking away part of the magnetic beads in the holes when the supernatant of the magnetic beads in the holes is sucked and discarded (the target product is on the magnetic beads), which ultimately leads to poor experimental results.
[0032] The temperature-controlled magnetic module in this technical solution also has a magnetic unit 93 that effectively absorbs the magnetic beads in each hole of the reaction container 100 and a heating plate 921 that can ensure that the reaction container 100 is in a constant temperature control state, so that during the washing and hybridization process, the sample (i.e., the sample liquid) is kept at a constant temperature, and the magnetic attraction is better used to remove non-specific gene fragments, so as to obtain more target fragments and improve the quality of sequencing data. In addition, the temperature-controlled magnetic module of the utility model is also conducive to realizing the automation of the hybridization capture library construction process, reducing the probability of library construction failure caused by manual operation, and solving the problem of large differences in libraries between batches due to manual operation.
[0033] In order to ensure full utilization of heat, in a specific embodiment, a heat insulating layer 923 is provided on the bottom surface of the heating plate 921 to prevent the heat generated by the heating plate 921 from escaping to the side away from the sample.
[0034] In a specific embodiment, the PCR plate carrier 922 is made of aluminum. Aluminum has high thermal conductivity and can more efficiently conduct the heat generated by the heating plate 921 to the sample liquid in each hole of the reaction container 100, thereby ensuring efficient constant temperature control of the sample liquid. At the same time, the price of aluminum is relatively low, which can reduce the manufacturing cost of the equipment.
[0035] In some embodiments, a magnetic column receiving groove (not shown in the figure) is formed on the lower end surface of the PCR plate carrier 922, and each of the magnetic columns 9311 can extend into the magnetic column receiving groove when driven to rise. A magnetic column receiving groove recessed from bottom to top is formed on the bottom side of the PCR plate carrier 922 to ensure that each magnetic column 9311 can extend upward to a certain height, which can ensure that the magnetic column 9311 adsorbs the magnetic beads in each hole body on the side wall. It can be understood that the heating plate 921 and the thermal insulation layer 923 are respectively formed with through holes (not shown in the figure, not labeled) corresponding to each magnetic column 9311.
[0036] In a specific embodiment, a total of 24 magnetic pillars 9311 are arranged on one magnetic pillar card plate 931 , and correspondingly, the reaction container 100 is a 96-well plate.
[0037] In order to ensure temperature control, in some embodiments, the PCR plate carrier 922 is provided with an insulation layer 924 on the peripheral side walls, which can prevent heat from being transferred to the external environment and ensure the temperature consistency of the PCR plate carrier. Specifically, the bottom of the aforementioned PCR plate carrier 922 has a carrier bottom plate 9224 that matches and fits the top surface of the heating plate 921, and its peripheral walls will protrude from the area where each bearing hole 9221 is located, that is, forming a step. At this time, the aforementioned insulation layer 924 can adaptively have a corresponding step to achieve reliable wrapping of the position.
[0038] In some embodiments, air slots 9223 are formed in the area between four adjacent bearing holes 9221 of the PCR plate carrier 922. The arrangement of each air slot 9223 can make the hole wall thickness of each bearing hole 9221 as consistent as possible, so that the thermal conductivity at each position of the PCR plate carrier 922 can be basically consistent, thereby further ensuring the constant temperature effect. It should be noted that the notch of the aforementioned air slot 9223 can be facing upward or downward, and the specific direction can be reasonably selected according to actual needs.
[0039] In some embodiments, the temperature-controlled magnetic module further includes a lifting drive assembly 94, wherein the lifting drive assembly 94 has a lifting column 941, wherein the lifting column 941 is used to drive the magnetic column card plate 931 to lift and lower. The aforementioned lifting drive assembly 94 can specifically adopt a through-type screw motor, in which case the aforementioned lifting column 941 is also the screw of the through-type screw motor, which has a simple structure and flexible control. In a specific embodiment, a drive component mounting bracket 942 is also fixedly connected to the bracket 91, and a fixed part of the lifting drive assembly 94, such as a motor housing, is fixedly connected to the drive component mounting bracket 942.
[0040] In a specific embodiment, the magnetic column clamping plate 931 has an open cavity facing upward, and each magnetic column 9311 is assembled and positioned in each through hole on the magnetic column clamping plate 931 from bottom to top. A magnetic column sealing plate 934 is also assembled on the aforementioned open cavity to prevent each magnetic column 9311 from escaping from the through hole.
[0041] In some embodiments, a buffer plate 932 is provided below the magnetic column clamping plate 931, and there is a gap between the top surface of the buffer plate 932 and the bottom surface of the magnetic column clamping plate 931 (when including a magnetic column sealing plate 934, it is the bottom surface of the magnetic column sealing plate 934), and a plurality of elastic members 933 are arranged at intervals in the gap, and the free end of the lifting column 941 is connected to the bottom surface of the buffer plate 932. Specifically, the elastic member 933 is a metal coil spring, and the upper and lower ends of the metal coil spring are fixedly connected to the magnetic column clamping plate 931 and the buffer plate 932, respectively, and a plurality of the elastic members 933 are respectively arranged at the four corner areas of the magnetic column clamping plate 931.
[0042] In this technical solution, by arranging a plurality of elastic members 933 between the magnetic column card plate 931 and the buffer plate 932, on the one hand, the horizontal adjustment of the magnetic column card plate 931 can be achieved, thereby ensuring that each magnetic column 9311 can be accurately located at the side wall position of each hole body when being driven to rise, and the magnetic beads in the hole body can be reliably adsorbed on the side wall of the hole body, thereby preventing some magnetic columns from being not raised to the side wall of the hole body but close to the bottom wall of the hole body due to the unevenness of the magnetic column card plate 931, resulting in the loss of some magnetic beads in the hole when the pipette removes the supernatant of the magnetic beads (the target product is on the magnetic beads), and finally causing the experimental results to deteriorate; on the other hand, the aforementioned elastic member 933 can also prevent the magnetic column 9311 from being damaged by rigid collision with the PCR plate carrier 922 during the process of the magnetic column card plate 931 rising to adsorb the magnetic beads, especially when the material of the PCR plate carrier 922 is aluminum, it can effectively prevent the magnetic column 9311 from rigidly colliding with the aluminum to produce pits, thereby ensuring the thermal conductivity consistency of the PCR plate carrier 922.
[0043] In some embodiments, the left and right ends of the buffer plate 932 are respectively slidably connected to the bracket 91 through the slide rail 95. Specifically, the left and right ends of the buffer plate 932 are respectively provided with a slider (not marked in the figure), and the slider is slidably connected to the slide rail 95. Due to the leveling effect of the aforementioned buffer plate 932, the matching accuracy between the slider and the slide rail 95 at this point can be reduced, thereby reducing the module manufacturing cost.
[0044] According to an embodiment of the utility model, there is also provided an automated workstation for constructing a sequencing hybridization capture library, comprising the above-mentioned temperature-controlled magnetic suction module.
[0045] The following describes a method for using the temperature-controlled magnetic module of the present invention in conjunction with a specific embodiment.
[0046] 1. Introduction to the hybridization capture process of the library
[0047] The purpose of hybridization washing is to obtain the target fragments required for the project (such as drug-targeted target gene fragments).
[0048] Principle of hybridization wash: The hybridization process has bound the target fragment in the constructed small fragment library to the probe. Hybridization wash is to complementarily bind the biotin on the probe with the streptavidin on the capture magnetic beads, and then the magnetic beads are adsorbed on the magnetic frame (that is, the aforementioned magnetic suction unit 93, the same below) to capture the desired target fragment, and wash away the weakly bound and non-target fragments, and only obtain the firmly bound target fragment.
[0049] 2. Reagents required for hybridization:
[0050] ① Capture wash solution I (1): washes away most of the unbound non-target fragments.
[0051] ② Capture wash solution II (2): wash away unbound non-target fragments.
[0052] ③ Capture wash solution III (3): wash away unbound non-target fragments.
[0053] ④Hot washing solution (4): wash away the loosely bound fragments.
[0054] ⑤ Magnetic bead cleaning solution (5): activates the capture magnetic beads.
[0055] 3. Automated hybridization washing process:
[0056] ①Activation of capture magnetic beads
[0057] Take 100 μL of captured magnetic beads and add them to the PCR plate (i.e. the reaction container 100, hereinafter the same) (which is built into the temperature-controlled magnetic absorption module of the present invention), and raise the magnet (i.e. the magnetic column 9311, hereinafter the same) to absorb the magnetic beads for 2 minutes (such as Figure 3 ), discard the supernatant, lower the magnet (such as Figure 2 );
[0058] Add another 200 μL of reagent (5) to the PCR plate, pipette and mix, raise the magnet to adsorb the magnetic beads for 2 min, discard the supernatant, and lower the magnet;
[0059] Add another 200 μL of reagent (5) to the PCR plate, pipette and mix, raise the magnet to adsorb the magnetic beads for 2 min, discard the supernatant, and lower the magnet;
[0060] Add 100 μL of reagent (5) to the PCR plate, pipette and mix, raise the magnet to adsorb the magnetic beads for 2 min, discard the supernatant, and lower the magnet;
[0061] ② Combine with capture magnetic beads to catch the target fragment
[0062] The PCR tube with activated capture magnetic beads is placed in a temperature-controlled magnetic module (non-magnetic state, 47°C). The hybridization library is added to the PCR plate wells containing capture magnetic beads and mixed by pipetting. The magnetic beads are allowed to bind for 45 minutes and mixed quickly every 15 minutes to ensure thorough mixing.
[0063] ③ Elution
[0064] After 45 minutes, keep the PCR plate in the temperature-controlled magnetic module (non-magnetic state, such as Figure 2 ), add 100 μL of preheated reagent (1) to the PCR well, mix by pipetting, add 200 μL of preheated reagent (4), mix by pipetting, and set the temperature-controlled magnetic module (non-magnetic state, such as Figure 2 ) for 5 minutes, then raise the magnetic column of the temperature control magnetic module and save the magnetic column adsorption state (such as Figure 3), discard the supernatant; the magnetic column descends, the automatic temperature-controlled magnetic module is in a non-magnetic state, and then add 200 μL of preheated reagent (4), blow and aspirate to mix, let it stand for 5 minutes, the magnetic column rises, the temperature-controlled magnetic module is in a magnetic state, and discard the supernatant; the magnetic column descends, the temperature-controlled magnetic module is in a non-magnetic state, add 200 μL of reagent (1) (no preheated reagent), blow and aspirate to mix for 2 minutes, the magnetic column rises, the temperature-controlled magnetic module is in a magnetic state, and discard the supernatant; the magnetic stand descends; add 200 μL of reagent (2), blow and aspirate to mix, the magnetic column is adsorbed, and the stand discards the supernatant; add 200 μL of reagent (3), preheat for 30 seconds, adsorb on the magnetic column, and discard the supernatant; add 45 μL ddH2O (without preheating) to dissolve the magnetic beads, mix; adsorb on the magnetic column, discard the supernatant, and aspirate the supernatant into a PCR tube to end.
[0065] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
[0066] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. A temperature-controlled magnetic module, characterized in that: The invention comprises a support (91), wherein a heating plate (921) is arranged on the top of the support (91), a PCR plate carrier (922) capable of conducting heat is arranged on the top surface of the heating plate (921), the PCR plate carrier (922) has bearing holes (9221) whose number and shape match those of the holes of the reaction container (100), and a magnetic attraction unit (93) is also arranged on the support (91), wherein the magnetic attraction unit (93) comprises a magnetic column card plate (931), wherein the magnetic column card plate (931) can be driven to rise and fall, and wherein the top surface of the magnetic column card plate (931) has a plurality of magnetic columns (9311) arranged in a square array, and any one of the magnetic columns (9311) can adsorb the magnetic beads in four adjacent holes of the reaction container (100) onto the side walls of the holes.
2. The temperature-controlled magnetic module according to claim 1, characterized in that: The bottom surface of the heating plate (921) is provided with a heat insulating layer (923); and / or the material of the PCR plate carrier (922) is aluminum.
3. The temperature control magnetic module according to claim 2, characterized in that: The lower end surface of the PCR plate carrier (922) is formed with a magnetic column receiving groove (9222), and each of the magnetic columns (9311) can extend into the magnetic column receiving groove (9222) when being driven to rise.
4. The temperature control magnetic module according to claim 2, characterized in that: A heat-insulating layer (924) is provided on the peripheral side wall of the PCR plate carrier (922).
5. The temperature control magnetic module according to claim 2, characterized in that: An air groove (9223) is formed in the area between four adjacent bearing holes (9221) of the PCR plate carrier (922).
6. The temperature-controlled magnetic module according to any one of claims 1 to 5, characterized in that: It also includes a lifting drive assembly (94), wherein the lifting drive assembly (94) has a lifting column (941), and the lifting column (941) is used to drive the magnetic column clamping plate (931) to rise and fall.
7. The temperature control magnetic module according to claim 6, characterized in that: A buffer plate (932) is arranged below the magnetic column clamping plate (931), a gap is provided between the top surface of the buffer plate (932) and the bottom surface of the magnetic column clamping plate (931), and a plurality of elastic members (933) are arranged at intervals in the gap, and a free end of the lifting column (941) is connected to the bottom surface of the buffer plate (932).
8. The temperature control magnetic module according to claim 7, characterized in that: The elastic member (933) is a metal coil spring, the upper and lower ends of which are respectively fixedly connected to the magnetic column clamping plate (931) and the buffer plate (932), and a plurality of the elastic members (933) are respectively arranged at the four corner areas of the magnetic column clamping plate (931).
9. The temperature control magnetic module according to claim 7, characterized in that: The left and right ends of the buffer plate (932) are slidably connected to the bracket (91) via slide rails (95) respectively.
10. An automated workstation for constructing a sequencing hybridization capture library, characterized in that: It comprises the temperature-controlled magnetic module according to any one of claims 1 to 9.