Saliva sheath collecting device for brown planthopper
By designing a brown planthopper saliva sheath collection device, using the attractive sandwich structure of the package and seal, the problems of low saliva sheath collection efficiency and ineffective experimental results in the prior art are solved, and efficient collection and experimental results of a single brown planthopper saliva sheath are achieved accuracy.
Patent Information
- Application Number
- CN202421423279.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-21
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-06-21
AI Technical Summary
The prior art cannot accurately study the specific changes in the number and morphology of brown planthopper saliva sheaths after RNAi treatment, and death is prone to occur in the breeding of multi-headed brown planthoppers, resulting in invalid saliva sheath collection results.
A saliva sheath collection device for brown planthoppers is designed, including a container tube and a package with both openings at the ends. The seal forms an attractive interlayer with the closure to encapsulate and collect the saliva sheath of a single brown planthopper to prevent dead lice from affecting the results.
The efficient and accurate collection of saliva sheaths of a single brown planthopper is achieved, avoiding experimental waste caused by dead lice, and improving the rigor and efficiency of the experiment.
Smart Images
Figure CN222236317U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of artificial breeding of brown planthoppers, in particular to a saliva sheath collection device for brown planthoppers. Background Art
[0002] Brown planthopper (Nilaparvata lugens (Stal)) is a piercing-sucking rice pest that uses its needle-like mouth needle to pierce rice tissues, causing malnutrition, fading, yellowing, and even withering or death of the entire plant. Brown planthoppers feed on the sap in the phloem of deep rice tissues. As the mouth needle pierces the epidermis and moves, it secretes saliva to form a salivary sheath that wraps around the mouth needle. The salivary sheath is an essential structure for the piercing and withdrawal of the mouth needle, and contains a variety of proteins. In recent years, domestic and foreign studies have found that brown planthoppers often use the secreted saliva components to target host plant target proteins, destroy the physical barriers of the host plant, inhibit the resistance signal transduction of the host plant, and inhibit the toxic effects of the host's secondary metabolites, thereby achieving the purpose of feeding damage.
[0003] Due to the irrational use of chemical pesticides, the damage of brown planthoppers to rice crops has tended to increase, and their resistance to commonly used chemical pesticides has gradually increased, making prevention and control more difficult. RNA interference (RNAi) is a gene silencing phenomenon caused by a small non-coding RNA composed of 20 to 30 nucleotides (nt) that is highly conserved in organisms. As a tool for gene silencing, RNAi technology has been widely used in the study of crop pest control. Based on RNAi technology, the expression of salivary sheath protein genes in insects is inhibited by feeding, injection and plant-mediated RNAi, and then the morphology and quantity changes of salivary sheaths secreted by brown planthoppers are detected, thereby exploring its important role in the formation and feeding of salivary sheaths of brown planthoppers, in order to provide theoretical guidance for the research on pest control in my country. Therefore, the development of a device suitable for collecting and studying the morphology and quantity of salivary sheaths of brown planthoppers treated with RNAi is the key to the experiment.
[0004] In the prior art, 20-30 brown planthoppers are usually placed in a larger container to obtain a large amount of saliva and salivary sheaths of the brown planthoppers, so as to perform omics sequencing or protein immunoblotting experiments. However, the prior art cannot accurately study the specific changes in the number and morphology of salivary sheaths of brown planthoppers after RNAi treatment. This is because one or more brown planthoppers may die unexpectedly during the experiment. When multiple brown planthoppers are collected together, the experimenters cannot distinguish which salivary sheaths are left by dead brown planthoppers. Dead brown planthoppers represent viral factors lurking in their bodies before the experiment, which will affect the quality of the salivary sheaths secreted by them. Therefore, as long as there is a dead brown planthopper in the container, the collection results of the salivary sheaths of all brown planthoppers in the entire breeding container will be unusable, which will not only cause a lot of waste, but also fail to achieve the purpose of the experiment. Therefore, it is necessary to provide a device that can efficiently collect the salivary sheaths of each brown planthopper. Utility Model Content
[0005] In view of the deficiencies in the prior art, the purpose of the utility model is to provide a salivary sheath collection device for brown planthoppers, aiming to solve the technical problem in the prior art of lacking an efficient collection device for the salivary sheath of a single brown planthopper.
[0006] In order to achieve the above purpose, the utility model is implemented through the following technical solutions:
[0007] A device for collecting salivary sheaths of brown planthoppers comprises a accommodating tube with openings at both ends, and a packaging member located in the accommodating tube, wherein the packaging member is used to encapsulate brown planthoppers, the packaging member is open at both ends, and the two ends of the packaging member are respectively flush with the two ends of the accommodating tube, one of the open ends of the accommodating tube is provided with a sealing member and a closing member in sequence from the accommodating tube outward, an attraction interlayer is formed between the sealing member and the closing member, the attraction interlayer is used to accommodate artificial breeding liquid, when the stylet of the brown planthopper pierces the sealing member to eat the artificial breeding liquid, the stylet of the brown planthopper is retracted to the packaging member, the sealing member blocks and collects the salivary sheaths of the brown planthopper, the other open end of the accommodating tube is covered with a sealing member for preventing the brown planthoppers from escaping, the opening size of the packaging member close to one end of the sealing member is larger than the opening size close to one end of the sealing member, and the opening diameter of the packaging member close to one end of the sealing member is 5 mm to 6 mm.
[0008] According to one aspect of the above technical solution, the sealing member and the closing member are both made of sealing films.
[0009] According to one aspect of the above technical solution, the sealing member is made of wet gauze, and the containing tube is made of PVC hard material.
[0010] According to one aspect of the above technical solution, there are a plurality of the packaging components, and the plurality of the packaging components are adjacently arranged in the containing tube.
[0011] According to one aspect of the above technical solution, the saliva sheath collection device of brown planthopper further comprises a sampling tube rack, and a plurality of accommodating holes for placing the accommodating tubes are arranged in an array on the upper surface of the sampling tube rack.
[0012] According to one aspect of the above technical solution, the accommodation tube is placed in the accommodation hole, and the side of the accommodation tube provided with the sealing member is placed downward.
[0013] According to one aspect of the above technical solution, there is a gap between the accommodating tube and the bottom surface of the accommodating hole.
[0014] Compared with the prior art, the beneficial effects of the present invention are:
[0015] A packaging piece for packaging brown planthoppers is provided, and the packaging piece is placed in a containing tube, a sealing piece and a closing piece are first provided in sequence at one end of the containing tube, an attracting interlayer is formed between the sealing piece and the closing piece, and an opening end of the packaging piece is pressed against the sealing piece, then the brown planthoppers are placed in from the other opening end of the packaging piece, and the containing tube is quickly sealed with a sealing piece so that the other opening end of the packaging piece is pressed against the sealing piece, so that the brown planthoppers are contained in the packaging piece, an artificial breeding liquid for attracting the brown planthoppers to feed is placed in the attracting interlayer, and the sealing piece is sealed on the sealing piece, To prevent the loss of artificial feeding liquid, the brown planthopper is attracted by the artificial feeding liquid and pierces the sealing member through the stylet, so that the stylet enters the artificial feeding liquid, thereby allowing the brown planthopper to suck the artificial feeding liquid. When the brown planthopper retracts the stylet into the packaging member, the salivary sheath will remain on the sealing member due to the obstruction of the sealing member. After the test, the surviving brown planthopper is recovered, and the sealing member is carefully cut off with a blade. After staining with Coomassie Brilliant Blue staining solution, the salivary sheath can be easily observed under an optical microscope, and then the salivary sheath of the brown planthopper is collected by the device;
[0016] The size of the seal is designed to facilitate a single brown planthopper to pierce the seal without escaping from the other end, because after the brown planthopper enters from the narrow end, it will move toward the wide end due to the attraction of the artificial feeding liquid and phototropism;
[0017] The utility model has simple materials and is easy to manufacture, and can efficiently collect the salivary sheath of a single brown planthopper. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:
[0019] Figure 1This is a schematic diagram of the explosion structure of the accommodation pipe in the embodiment of the utility model;
[0020] Figure 2 This is a schematic diagram of the structure of the containing tube and the packaging member after the assembly is completed in the embodiment of the utility model;
[0021] Figure 3 This is a schematic diagram of the structure of the sampling tube rack in the embodiment of the utility model;
[0022] Figure 4 It is a cross-sectional schematic diagram of the sealing member, the suction interlayer, and the closing member in the embodiment of the utility model;
[0023] The following specific implementation manner will further illustrate the present utility model in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION
[0024] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. A plurality of embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.
[0025] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.
[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.
[0027] Embodiment 1
[0028] See also Figures 1 to 4, shown is a saliva sheath collection device for brown planthoppers in an embodiment of the utility model, comprising a receiving tube 10 with openings at both ends, and a packaging member 20 located in the receiving tube 10, the packaging member 20 is used to encapsulate brown planthoppers, the packaging member 20 has openings at both ends, and the two ends of the packaging member 20 are respectively flush with the two ends of the receiving tube 10, one of the open ends of the receiving tube 10 is provided with a sealing member 30 and a closing member 40 in sequence from the receiving tube 10 outward, an attraction interlayer 60 is formed between the sealing member 30 and the closing member 40, and the attraction interlayer 60 is used to The artificial breeding liquid is contained. When the stylet of the brown planthopper pierces the sealing member 30 to feed on the artificial breeding liquid, the stylet of the brown planthopper is retracted into the packaging member 20, and the sealing member 30 blocks and collects the salivary sheath of the brown planthopper. The other open end cover of the accommodating tube 10 is provided with a sealing member 50 for preventing the brown planthopper from escaping. The opening size of the packaging member 20 near one end of the sealing member 30 is larger than the opening size near one end of the sealing member 50. The opening of the packaging member 20 near one end of the sealing member 50 is used for the entry of the brown planthopper.
[0029] It can be understood that the utility model provides a package 20 for encapsulating brown planthoppers, and places the package 20 in the housing tube 10, firstly sequentially arranges a sealing member 30 and a closing member 40 at one end of the housing tube 10, forms an attraction interlayer 60 between the sealing member 30 and the closing member 40, and places an opening end of the package 20 against the sealing member 30, then places the brown planthoppers in from the other opening end of the package 20, quickly seals the housing tube 10 with the sealing member 50, so that the other opening end of the package 20 is against the sealing member 50, so that the brown planthoppers are contained in the package 20, and an artificial feed for attracting the brown planthoppers to feed is placed in the attraction interlayer 60. The artificial breeding liquid is encapsulated on the sealing member 30 with a sealing member 40 to prevent the artificial breeding liquid from being lost. The brown planthopper is attracted by the artificial breeding liquid and pierces the sealing member 30 through the mouth needle, so that the mouth needle enters the artificial breeding liquid, so that the brown planthopper sucks the artificial breeding liquid. When the brown planthopper retracts the mouth needle into the encapsulating member 20, the salivary sheath will remain on the sealing member 30 due to the obstruction of the sealing member 30. After the test, the surviving brown planthopper is recovered, and the sealing member is carefully cut off with a blade. After staining with Coomassie Brilliant Blue staining solution, the salivary sheath can be easily observed under an optical microscope, and then the salivary sheath of the brown planthopper is collected by the device;
[0030] It should be noted that the formation principle of the salivary sheath is as follows: when insects probe, the salivary glands secrete gelatinous salivary proteins, which solidify into a sheath structure due to the influence of external environmental oxidation. On the one hand, the sheath structure formed by the gelatinous saliva will seal the cell wounds caused by the probing of the mouth needle, preventing the outflow of cell contents and triggering the immune response of the plant; on the other hand, the gelatinous saliva can lubricate the mouth needle to ensure that it can smoothly pierce the tissue cells and reach the feeding site. During the test, brown planthoppers will feed on the artificial feeding liquid repeatedly, and will secrete new salivary sheaths each time they feed. Therefore, during a test, multiple salivary sheaths will be left on the seal.
[0031] The size of the encapsulation member 20 is to facilitate the brown planthopper to pierce the sealing member 30 and prevent the brown planthopper from escaping from the other end, because after the brown planthopper enters from the narrow end, it will move toward the wide end due to the attraction of the artificial breeding liquid and phototropism;
[0032] The utility model has simple materials and is easy to manufacture, and can efficiently collect the salivary sheaths of brown planthoppers treated with RNAi.
[0033] Specifically, in this embodiment, the sealing member 30 and the closing member 40 are both made of sealing films; the artificial feeding liquid can be obtained through commercial sales and other means, and the specific components are as follows:
[0034]
[0035] The sealing member 50 is made of wet gauze, and the containing tube 10 is made of PVC hard material; there are multiple packaging members 20, and the multiple packaging members 20 are adjacently arranged in the containing tube 10.
[0036] It can be understood that the sealing film has a unique permeability, good water vapor permeability and strong corrosion resistance. At the same time, the sealing film can collect the salivary sheath of brown planthoppers. Brown planthoppers can pierce the sealing film through the mouth needle to feed on the artificial feeding liquid in the attracting interlayer 60. The sealing member 50 uses a moist gauze to quickly block the opening of the packaging member 20 to prevent the brown planthoppers from escaping. The moist gauze has a strong sealing performance and is not easy to be punctured and broken, so as to prevent the brown planthoppers from damaging the sealing member 50. In this embodiment, there are three packaging members 20, so one receiving tube 10 can collect the saliva of three brown planthoppers, which greatly improves the collection efficiency. The receiving tube 10 is made of PVC hard material and can accommodate and protect multiple adjacent packaging members 20. The PVC hard material has a certain elasticity. When the three packaging members 20 are placed in the receiving tube 10 in a triangle, the outer wall of the packaging member 20 can be tightly attached to the inner wall of the receiving tube 10 to form a clamping effect, thereby preventing the packaging member 20 from shaking and affecting the brown planthopper.
[0037] It should be noted that since each package contains an RNAi-treated brown planthopper, the survival status of each brown planthopper can be understood. The data of the salivary sheaths secreted by the brown planthoppers that died unexpectedly during the test period can be discarded. We only collect the salivary sheaths secreted by each surviving RNAi-treated brown planthopper during the entire test period, and then conduct the next step of research and analysis, which can not only achieve the purpose of the test, but also improve the rigor of the test. Due to the pressure, the end of the package 20 close to the seal 30 will leave a circular imprint (diameter 7 mm) on the seal, and the salivary sheath will appear blue after being stained with Coomassie blue brilliant blue staining solution. Under a microscope, it can be seen that the salivary sheaths secreted by each brown planthopper on the seal 30 are within the circular imprint, which is conducive to observing and counting the morphology and number of salivary sheaths secreted by each brown planthopper. When the experiment needs to set up multiple technical repetitions, since one holding tube can hold 3 packages and the collection device is easy to make, we can increase the technical repetitions by making multiple collection devices, thereby improving the collection efficiency.
[0038] There is no need to place too much artificial breeding liquid. Each containing tube 10 is filled with 150 μl of artificial breeding liquid to prevent the two sealing films from squeezing the artificial breeding liquid, causing the artificial breeding liquid to overflow and wet the sealing film, thereby reducing the viscosity of the sealing film to the containing tube 10.
[0039] It should be noted that the sealing film is a fully enclosed material. The sealing member 30 and the closing member 40 are both made of sealing films, which can preserve the artificial breeding liquid in the suction interlayer 60 intactly.
[0040] Preferably, a pipette tip can be used as the packaging member 20. The pipette tip has a wide-mouth end with a larger cross-sectional size and a tip end with a smaller cross-sectional size. The pipette tip generally has a capacity of 1 mL. After it is shortened to 33 mm from the tip end, it is adapted to the size of the containing tube 10. The tip end is used to place brown planthoppers (about 1 mm to 3 mm in size).
[0041] Furthermore, the saliva sheath collection device of the brown planthopper also includes a sampling tube rack 70, and the upper surface of the sampling tube rack 70 is arrayed with a plurality of receiving holes 80 for placing the receiving tube 10; the receiving tube 10 is placed with the side provided with the sealing member 30 facing downward; there is a gap between the receiving tube 10 and the bottom surface of the receiving hole 80.
[0042] It can be understood that in order to facilitate centralized collection, a sampling tube rack 70 is used to collect multiple receiving tubes 10 into the receiving hole 80, and then processed in batches, thereby improving the collection efficiency. The receiving tube 10 is placed with the side having the seal 30 facing downward, which can facilitate the brown planthopper to lie on the seal 30, pierce the seal 30 and lick the artificial breeding liquid in the attraction interlayer 60, thereby improving the collection efficiency. The presence of a gap between the receiving tube 10 and the bottom surface of the receiving hole 80 can prevent the artificial breeding liquid in the attraction interlayer 60 from contacting the bottom surface of the receiving hole 80 through the sealing member 40, thereby causing the artificial breeding liquid to seep out.
[0043] Preferably, the lower end of the sampling tube rack 70 can be penetrated to expose one end of the accommodating tube 10 with the attraction interlayer 60. Since brown planthoppers are phototactic, it is convenient for brown planthoppers to climb onto the sealing member 30 and suck the artificial breeding liquid in the attraction interlayer 60, so as to facilitate the experimenters to observe the situation of brown planthoppers.
[0044] Preferably, this embodiment provides optimal dimensions of each component, including: containing tube: inner diameter 20 mm, outer diameter 23 mm, length 33 mm; packaging member: diameter 7 mm, length 33 mm; sealing member and closure member after stretching: length 50 mm, width 50 mm; sealing member: density 30*30, representing warp yarn 30*weft yarn 30; containing hole: upper aperture 27 mm, lower aperture 25 mm.
[0045] It should be noted that since the sealing member 30 and the closing member 40 are both made of sealing film, two layers of sealing film are covered on the end of the receiving tube so that the width of the end of the receiving tube 10 plus the two layers of sealing film is larger than the lower hole diameter of the receiving hole 80 of 25 mm, and the receiving tube 10 can be stuck in the hole wall of the receiving hole 80, so that there is a gap between the receiving tube 10 and the bottom surface of the receiving hole 80.
[0046] In summary, the saliva sheath collection device for brown planthopper in the above embodiments of the utility model has simple materials, is easy to manufacture, and can efficiently collect the saliva sheath of a single brown planthopper.
[0047] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0048] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.
Claims
1. A saliva sheath collection device for brown planthopper, characterized in that: The invention comprises a housing tube with openings at both ends, and a packaging member located in the housing tube, wherein the packaging member is used to package brown planthoppers, the packaging member is opened at both ends, and the two ends of the packaging member are respectively flush with the two ends of the housing tube, one of the open ends of the housing tube is provided with a sealing member and a closing member in sequence from the housing tube outward, an attraction interlayer is formed between the sealing member and the closing member, the attraction interlayer is used to accommodate artificial breeding liquid, the other open end cover of the housing tube is provided with a sealing member for preventing the brown planthoppers from escaping, the opening size of the packaging member close to one end of the sealing member is larger than the opening size close to one end of the sealing member, and the opening diameter of the packaging member close to one end of the sealing member is 5 mm to 6 mm.
2. The saliva sheath collection device of brown planthopper according to claim 1, characterized in that: The sealing member and the closing member are both made of sealing films.
3. The saliva sheath collection device of brown planthopper according to claim 1, characterized in that: The sealing member is made of wet gauze, and the containing tube is made of PVC hard material.
4. The saliva sheath collection device of brown planthopper according to claim 1, characterized in that: There are a plurality of the packaging members, and the plurality of the packaging members are adjacently arranged in the housing tube.
5. The saliva sheath collection device of brown planthopper according to claim 1, characterized in that: The saliva sheath collection device for brown planthoppers also includes a sampling tube rack, and a plurality of accommodating holes for accommodating the accommodating tubes are arranged in an array on the upper surface of the sampling tube rack.
6. The saliva sheath collection device of brown planthopper according to claim 5, characterized in that: The accommodating tube is placed in the accommodating hole, and the side of the accommodating tube provided with the sealing member is placed downward.
7. The saliva sheath collection device of brown planthopper according to claim 6, characterized in that: There is a gap between the accommodating tube and the bottom surface of the accommodating hole.