Full-automatic microcarrier cleaning device

Abstract

The utility model discloses a full-automatic microcarrier cleaning device which comprises a cleaning tank, a liquid storage barrel, a waste liquid barrel, a collecting bottle, a magnetic stirrer, an electronic scale and a controller, the magnetic stirrer is arranged on the top of the cleaning tank, the cleaning tank is arranged on the upper portion of the electronic scale, and the lower portion of one side of the cleaning tank is connected with a silicone tube connector. The silica gel pipe connector is connected with a liquid inlet pipe A and a liquid outlet pipe A through liquid inlet and outlet pipes, the liquid inlet pipe A and the liquid outlet pipe A are correspondingly communicated with a liquid storage barrel and a collecting bottle respectively, the liquid storage barrel is further communicated with the upper portion of a cleaning tank through a liquid inlet pipe B, and the bottom of the cleaning tank is communicated with a waste liquid barrel through a liquid outlet pipe B. A filter screen is further arranged on the inner bottom wall of the cleaning tank. Corresponding electromagnetic valves are respectively arranged on the liquid inlet pipe A, the liquid outlet pipe A, the liquid inlet pipe B and the liquid outlet pipe B; and the magnetic stirrer, the electronic scale and the electromagnetic valves on the pipelines are electrically connected with the controller. The utility model has the advantages that the cleaning time is shortened, the operation is simple, the cleaning efficiency is improved, the loss of cell viability is reduced, and the consistency and stability of the process are kept.

Description

Technical Field

[0001] The utility model relates to the technical field of microcarrier cleaning, in particular to a full-automatic microcarrier cleaning device. Background Art

[0002] The microcarrier cleaning devices currently on the market generally have the following problems: (1) The cleaning time is long; (2) After cleaning, the content of impurities such as serum is high. (3) The operation process is complicated and requires manual control. (4) The amount of cell loss is large and the viability decreases significantly. (5) There are large differences between batches and the proportion of unqualified products is high. (6) When the microcarriers are harvested after cleaning, there is a large amount of residue.

[0003] In view of the above problems, the applicant has repeatedly conducted manual tests on the existing microcarrier cleaning device and found that the root causes of the above problems are as follows:

[0004] (1) The degree of automation is not high, and most of the time it is manually controlled, which results in a long cleaning time.

[0005] (2) Impurities remain on the tank wall or pipeline, resulting in excessive levels of impurities such as serum;

[0006] (3) Some microcarriers settle at the bottom edge. At low speeds, the microcarriers cannot be suspended, especially after the liquid in the suspension is filtered dry. This is also the reason for the high content of impurities such as serum.

[0007] (4) During harvest, many microcarriers cannot be discharged from the tank. Utility Model Content

[0008] The utility model aims to provide a fully automatic microcarrier cleaning device to solve the technical problems existing in the background technology.

[0009] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0010] A fully automatic microcarrier cleaning device comprises a cleaning tank, a liquid storage barrel, a waste liquid barrel, a collecting bottle, a magnetic stirrer, an electronic scale and a controller, wherein the magnetic stirrer is arranged on the top of the tank body of the cleaning tank, the cleaning tank is arranged on the upper part of the electronic scale, a silicone tube joint is connected to the lower part of one side of the tank body of the cleaning tank, and the silicone tube joint and the tank bottom of the cleaning tank are at the same height, the silicone tube joint is connected to an inlet and outlet pipe, the inlet and outlet pipes are connected to a liquid inlet pipe A and a liquid outlet pipe A through a three-way pipe joint, the liquid inlet pipe A is connected to the liquid storage barrel, the liquid outlet pipe A is connected to the collecting bottle, the liquid storage barrel is also connected to a liquid inlet pipe B, and the inlet and outlet pipes are connected to the liquid storage barrel. An annular liquid spray pipe is connected to the liquid pipe B, and the annular liquid spray pipe is suspended inside the tank body of the cleaning tank near the upper end, and is sleeved on the outside of the stirring shaft of the magnetic stirrer. At least one liquid outlet pipe B is connected to the bottom of the tank body of the cleaning tank, and the other end of the at least one liquid outlet pipe B is connected to the waste liquid bucket. A filter is also arranged on the inner bottom wall of the tank body of the cleaning tank. Solenoid valve A, solenoid valve B, solenoid valve C and solenoid valve D are respectively arranged on the liquid inlet pipe A, liquid outlet pipe A, liquid inlet pipe B and liquid outlet pipe B, and the solenoid valve A, solenoid valve B, solenoid valve C and solenoid valve D are all electrically connected to the controller, and the controller is also electrically connected to the magnetic stirrer and the electronic scale.

[0011] Furthermore, a liquid inlet pipe C is connected to the top of the cleaning tank, and the other end of the liquid inlet pipe C is connected to the microcarrier container for inputting the microcarrier cell suspension in the microcarrier container into the cleaning tank.

[0012] Furthermore, an air intake pipe A is connected to the top of the tank body of the cleaning tank, and an electromagnetic valve E and an air filter A are arranged on the air intake pipe A, and the electromagnetic valve E is electrically connected to the controller.

[0013] Furthermore, an air intake pipe B is connected to the liquid storage barrel, and an electromagnetic valve F and an air filter B are arranged on the air intake pipe B, and the electromagnetic valve F is electrically connected to the controller.

[0014] Furthermore, the microcarrier container is also connected to an air intake pipe C, on which an electromagnetic valve G and an air filter C are arranged, and the electromagnetic valve G is electrically connected to the controller.

[0015] Furthermore, air filters D are also provided on the tops of the cleaning tank, the waste liquid barrel and the collecting bottle.

[0016] Furthermore, a low liquid level line is arranged on the outer wall of the cleaning tank, and the position of the low liquid level line is slightly higher than the position of the stirring blade of the magnetic stirrer.

[0017] Furthermore, a high liquid level line is arranged on the outer wall of the cleaning tank, and the position of the high liquid level line is lower than the position of the three-way pipe joint.

[0018] Furthermore, the cleaning tank is made of transparent glass, which is convenient for observing the color change of the solution in the tank and judging the cleaning status of the microcarrier suspension.

[0019] Compared with the prior art, the advantages of the utility model are as follows:

[0020] (1) Short cleaning time and simple operation: The entire cleaning process is fully automated, which is not only simple to operate, but also greatly improves the cleaning efficiency and effectively reduces the cleaning time.

[0021] (2) High cleanliness, low impurity content in serum, etc. after cleaning: After cleaning, there is almost no impurity or attachment residue on the wall of the cleaning tank and in each pipeline, which will not affect serum, etc.

[0022] (3) Small cell loss and high viability: The stirring paddle keeps running at a low speed continuously, the microcarriers always remain suspended, and the cells fall off the microcarriers less. At the same time, the continuous low-speed operation of the equipment avoids high-speed resuspension, reduces shear force, and greatly reduces the entire operation time, thereby effectively ensuring the viability of the cells.

[0023] (4) The consistency and stability of the process can be maintained: The entire cleaning process is automatically controlled by the built-in program of the device, avoiding the influence of human factors, thereby ensuring the consistency and stability of the cleaning process between batches.

[0024] (5) High microcarrier harvest rate: The harvest port is changed from the top to the bottom side, which can ensure that most of the microcarriers can be discharged smoothly from the tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solution in this embodiment, the following is a brief introduction to the drawings required for use in the description of the embodiment. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 It is a specific embodiment of the fully automatic microcarrier cleaning device of the utility model;

[0027] Explanation of the accompanying drawings: 1. cleaning tank; 2. liquid storage barrel; 3. waste liquid barrel; 4. collecting bottle; 5. microcarrier container; 6. magnetic stirrer; 7. electronic scale; 8. silicone tube joint; 9. high liquid level line; 10. low liquid level line; 11. filter screen; 12. liquid inlet and outlet pipes; 13. three-way pipe joint; 14. liquid inlet pipe A; 15. liquid outlet pipe A; 16. liquid inlet pipe B; 17. annular liquid spray pipe; 18. liquid outlet pipe B; 19. liquid inlet pipe C; 20. air inlet pipe A; 21. air inlet pipe B; 22. air inlet pipe C; 23. solenoid valve A; 24. solenoid valve B; 25. solenoid valve C; 26. solenoid valve D; 27. solenoid valve E; 28. air filter A; 29. ​​solenoid valve F; 30. air filter B; 31. solenoid valve G; 32. air filter C; 33. air filter D. DETAILED DESCRIPTION

[0028] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the following further describes how the present invention is implemented in conjunction with the accompanying drawings and specific implementation methods.

[0029] See also Figure 1, is a specific embodiment of the fully automatic microcarrier cleaning device of the utility model, comprising a cleaning tank 1, a liquid storage barrel 2, a waste liquid barrel 3, a collecting bottle 4, a magnetic stirrer 6, an electronic scale 7 and a controller (not shown in the figure), the magnetic stirrer 6 is arranged on the top of the tank body of the cleaning tank 1, the cleaning tank 1 is arranged on the upper part of the electronic scale 7, a silicone tube joint 8 is connected to the lower part of one side of the tank body of the cleaning tank 1, and the silicone tube joint 8 and the tank bottom of the cleaning tank 1 are at the same height, which can prevent residual liquid in the cleaning tank 1, the silicone tube joint 8 is connected to the liquid inlet and outlet pipes 12, the liquid inlet and outlet pipes 12 are connected to the liquid inlet pipe A14 and the liquid outlet pipe A15 through the three-way pipe joint 13, the liquid inlet pipe A14 is connected to the liquid storage barrel 2, the liquid outlet pipe A15 is connected to the collecting bottle 4, and the liquid storage barrel 2 is also connected to The liquid inlet pipe B16 is connected to a ring-shaped liquid spray pipe 17, and the ring-shaped liquid spray pipe 17 is suspended inside the tank body of the cleaning tank 1 near the upper end, and is sleeved on the outside of the stirring shaft of the magnetic stirrer 6. Two liquid outlet pipes B18 are connected to the bottom of the tank body of the cleaning tank 1, and the other ends of the two liquid outlet pipes B18 are connected to the waste liquid barrel 3. A filter screen 11 is also arranged on the inner bottom wall of the tank body of the cleaning tank 1. Solenoid valves A23, B24, C25 and D26 are respectively arranged on the liquid inlet pipe A14, the liquid outlet pipe A15, the liquid inlet pipe B17 and the liquid outlet pipe B18, and the solenoid valves A23, B24, C25 and D26 are all electrically connected to the controller, and the controller is also electrically connected to the magnetic stirrer 6 and the electronic scale 7. Among them, the cleaning tank 1 is used to clean the microcarriers; the liquid storage barrel 2 is used to transport the cleaning liquid into the cleaning tank 1; the waste liquid barrel 3 is used to receive the cleaning waste liquid discharged from the cleaning tank 1; the collecting bottle 4 is used to collect the cleaned microcarriers; the electronic scale 7 is used to weigh the liquid weight in the cleaning tank 1; the silicone tube joint 8 is used to ensure that the cleaned microcarriers in the cleaning tank 1 are completely emptied without any residue to avoid affecting the subsequent cleaning operation; the filter screen 11 is used to intercept the microcarriers to prevent them from entering the liquid outlet pipe B18; the liquid inlet pipe A14 is used to transport the cleaning liquid into the cleaning tank 1 and to contact with the microcarriers to be cleaned. The washed microcarrier suspension (mixed by microcarrier and cell culture fluid) is mixed; the liquid outlet pipe A15 is used to input the microcarriers cleaned in the cleaning tank 1 into the collecting bottle 4; the liquid inlet pipe B16 is used to spray the cleaning liquid into the cleaning tank 1 through the annular liquid spray pipe 17, and the inner wall of the cleaning tank 1 and the stirring shaft of the magnetic stirrer 6 are cleaned to avoid residual impurities on the inner wall of the cleaning tank 1 and the stirring shaft of the magnetic stirrer 6; the liquid outlet pipe B18 is used to discharge the cleaning waste liquid after cleaning in the cleaning tank 1 into the waste liquid barrel 3; the controller is used to control the automatic operation of the entire device.

[0030] Specifically, in this embodiment, a liquid inlet pipe C19 is also connected to the top of the tank body of the cleaning tank 1, and the other end of the liquid inlet pipe C19 is connected to the microcarrier container 5, which is used to input the microcarrier suspension (mixed by microcarriers and cell culture solution) in the microcarrier container 5 into the cleaning tank 1.

[0031] Specifically, in this embodiment, an air intake pipe A20 is connected to the top of the tank body of the cleaning tank 1, and an electromagnetic valve E27 and an air filter A28 are arranged on the air intake pipe A20, and the electromagnetic valve E27 is electrically connected to the controller. The air intake pipe A20 is used to introduce gas into the cleaning tank 1 to pressurize the cleaning tank 1 so that the liquid in the cleaning tank 1 can be discharged more quickly.

[0032] Specifically, in this embodiment, an air intake pipe B21 is connected to the liquid storage barrel 2, and an electromagnetic valve F29 and an air filter B30 are arranged on the air intake pipe B21, and the electromagnetic valve F29 is electrically connected to the controller. The air intake pipe B21 is used to introduce gas into the liquid storage barrel 2 to increase the pressure of the liquid storage barrel 2 to ensure that the cleaning liquid in the liquid storage barrel 2 can smoothly enter the cleaning tank 1.

[0033] Specifically, in this embodiment, an air intake pipe C22 is connected to the microcarrier container 5, and an electromagnetic valve G31 and an air filter C32 are provided on the air intake pipe C22, and the electromagnetic valve G31 is electrically connected to the controller. The air intake pipe C22 is used to introduce air into the microcarrier container 5 to increase the pressure in the container so that the liquid in the microcarrier container 5 can be discharged more quickly.

[0034] Specifically, in this embodiment, an air filter D33 is also provided on the top of the cleaning tank 1, the waste liquid barrel 3 and the collecting bottle 4; this air filter D33 is in a normally open state to avoid the risk of explosion due to excessive pressure in the cleaning tank 1, the waste liquid barrel 3 and the collecting bottle 4.

[0035] Specifically, in this embodiment, a low liquid level line 10 is provided on the outer wall of the cleaning tank 1 , and the position of the low liquid level line 10 is slightly higher than the position of the stirring blades of the magnetic stirrer 6 .

[0036] Specifically, in this embodiment, a high liquid level line 9 is provided on the outer wall of the cleaning tank 1 , and the position of the high liquid level line 9 is lower than the position of the three-way pipe joint 13 .

[0037] Specifically, in this embodiment, the cleaning tank 1 is made of transparent glass, so as to facilitate observation of the change in the liquid level in the cleaning tank 1 .

[0038] Specifically, in this embodiment, the magnetic agitator 6 is composed of a magnetic motor, a stirring shaft, and stirring blades. The magnetic motor is fixed to the top of the tank body of the cleaning tank 1 and is electrically connected to the controller. The stirring shaft and the stirring blades are both inserted into the cleaning tank 1, and the upper end of the stirring shaft is fixedly connected to the output end of the magnetic motor, and the lower end is fixedly connected to the stirring blades.

[0039] It can be understood that the number of the silicone tube joint 8, the inlet and outlet pipes 12, the three-way pipe joint 13, the inlet pipe A14, the outlet pipe A15 and the outlet pipe B18 can be set according to actual needs and is not limited to the number shown in the figure.

[0040] The fully automatic microcarrier cleaning device provided by the utility model can be used for microcarrier cleaning, antigen separation, and separation of cells and carriers after microcarrier digestion. When used for microcarrier cleaning, it can be used to recover microcarriers and clean microcarriers and attached components, such as removing serum residues.

[0041] The following is the operation process of the fully automatic microcarrier cleaning device of the utility model for cleaning microcarriers:

[0042] The first step is to open the electromagnetic valve G31 and introduce gas (such as air) into the microcarrier container 5 through the air inlet pipe C22, so that the suspension containing microcarriers in the microcarrier container 5 (a suspension formed by mixing microcarriers and cell culture solution) is input into the cleaning tank 1 through the liquid inlet pipe C19 until the liquid level reaches the high liquid level line 9, and then close the electromagnetic valve G31, and then record the weight of the cleaning tank 1 at this time through the electronic scale 7, and record it as A1, and automatically save this data A1 to the electronic scale 7 and the controller. At this moment, the data A1 recorded by the electronic scale 7 is the highest liquid level value that the cleaning solution can reach in the cleaning tank 1 during the microcarrier cleaning this time;

[0043] The second step is to open the electromagnetic valve D26 and the electromagnetic valve E27, and introduce gas into the cleaning tank 1 through the air inlet pipe A20, so that the liquid in the cleaning tank 1 (cell culture fluid excluding microcarriers) is discharged into the waste liquid bucket 3 through the liquid outlet pipe B18 until the liquid level drops to the low liquid level line 10, and then close the electromagnetic valve D26 and the electromagnetic valve E27, and then record the weight of the cleaning tank 1 at this time through the electronic scale 7, and record it as A2, and automatically save this data A2 to the electronic scale 7 and the controller. At this moment, the data A2 recorded by the electronic scale 7 is the lowest liquid level value that the cleaning liquid can reach in the cleaning tank 1 during the microcarrier cleaning this time;

[0044] In the third step, open the solenoid valve D26 and the solenoid valve E27 again, and introduce gas into the cleaning tank 1 through the air inlet pipe A20, so that the remaining liquid in the cleaning tank 1 (liquid except the microcarriers) is completely discharged into the waste liquid barrel 3 through the liquid outlet pipe B18, and then close the solenoid valve D26 and the solenoid valve E27 again. At this time, only the microcarriers to be cleaned are left in the cleaning tank 1. The microcarriers are specifically retained in the cleaning tank 1 by the filter net 11 arranged on the inner wall of the cleaning tank 1.

[0045] The fourth step is to open the electromagnetic valve A23 and the electromagnetic valve F29, and introduce gas (such as air) into the liquid storage barrel 2 through the air inlet pipe B21, so that the cleaning liquid in the liquid storage barrel 2 is sequentially input into the cleaning tank 1 through the liquid inlet pipe A14, the three-way pipe joint 13, the liquid inlet and outlet pipes 12, and the silicone pipe joint 8, until the value of the electronic scale 7 reaches A2, close the electromagnetic valve A23, and start the magnetic stirrer 6 to stir the cleaning liquid in the cleaning tank 1 at a low speed through the magnetic stirrer 6;

[0046] The fifth step is to open the solenoid valve C25, and continue to input the cleaning liquid into the cleaning tank 1 through the liquid inlet pipe B16 until the value of the electronic scale 7 reaches A1, and then close the solenoid valve C25 and the solenoid valve F29. In the process of inputting the cleaning liquid into the cleaning tank 1 through the liquid inlet pipe B16, the magnetic stirrer 6 keeps rotating at a low speed, and uses the cleaning liquid to continuously clean the microcarriers intercepted in the third step in the cleaning tank 1 until the cleaning time reaches the first cleaning time T1 preset by the system (the specific value of T1 is pre-set by the system program), then open the solenoid valve E27 and the solenoid valve D26, and introduce gas (such as air) into the cleaning tank 1 through the air inlet pipe A20, so that the waste liquid in the cleaning tank 1 after the first cleaning is discharged into the waste liquid barrel 3 through the liquid outlet pipe B18, until the value of the electronic scale 7 reaches A2, and then close the solenoid valve E27 and the solenoid valve D26; it should be noted here that in the process of discharging the cleaning waste liquid in this step, the magnetic stirrer 6 also keeps working;

[0047] The sixth step is to open the solenoid valve A23, the solenoid valve C25 and the solenoid valve F29, and introduce gas (such as air) into the liquid storage barrel 2 through the air inlet pipe B21, so that the liquid inlet pipe A14 and the liquid inlet pipe B16 simultaneously input the cleaning liquid into the cleaning tank 1, until the value of the electronic scale 7 reaches A1, close the solenoid valve A23, the solenoid valve C25 and the solenoid valve F29, and continue to clean the microcarriers in the cleaning tank 1 by the magnetic stirrer 6 until the cleaning time reaches the second cleaning time T2 preset by the system, open the solenoid valve E27 and the solenoid valve D26 again, and continue to introduce gas into the cleaning tank 1 through the air inlet pipe A20, so that the second cleaning waste liquid in the cleaning tank 1 is discharged into the waste liquid barrel 3 through the liquid outlet pipe B18, until the value of the electronic scale 7 reaches A2, and then close the solenoid valve E27 and the solenoid valve D26;

[0048] In the seventh step, the cleaning step of the sixth step is repeated N times, and at the end of the Nth time, the cleaning waste liquid in the cleaning tank 1 is completely emptied, wherein the value of N is preset by the system, and the value range of N is preferably 2 to 100. According to different cleaning requirements, the value of N will change accordingly. It should be noted that during the N times of cleaning process, the magnetic stirrer 6 is generally kept in working state, that is, its stirring paddle is always rotating until the number of cleaning times reaches the program setting value, and then it stops rotating.

[0049] Step 8. After the Nth cleaning waste liquid is completely drained, first open the solenoid valve A23, the solenoid valve C25 and the solenoid valve F29, and introduce gas into the liquid storage barrel 2 through the air inlet pipe B21, and simultaneously input the cleaning liquid into the cleaning tank 1 through the liquid inlet pipe A14 and the liquid inlet pipe B16, until the value of the electronic scale 7 reaches A1 or A2, then close the solenoid valve A23, the solenoid valve C25 and the solenoid valve F29, and then open the solenoid valve B24 and the solenoid valve E27, and introduce gas into the cleaning tank 1 through the air inlet pipe A20, so that the microcarriers and the cleaning liquid in the cleaning tank 1 enter the collecting bottle 4 together through the silicone tube joint 8, the liquid inlet and outlet pipe 12, and the liquid outlet pipe A15 in sequence, and the operation is terminated.

[0050] It should be noted that in the above operation steps, the opening and closing of each solenoid valve in the first to third steps are manually controlled, and in the subsequent steps, the opening and closing of each solenoid valve and the start and stop of the magnetic stirrer 6 are automatically controlled by the system.

[0051] In the above-mentioned operation steps, the purpose of the first and second steps is to use the electronic scale 7 to calibrate the weight corresponding to the high and low liquid levels of the suspension containing microcarriers in the cleaning tank 1, so that the subsequent system can directly read the value of the electronic scale 7 to automatically judge the high and low liquid levels in the cleaning tank 1, thereby automatically performing the cleaning operation.

[0052] For the calibration of the values ​​A1 and A2 in the first and second steps above, the calibration can be done in advance by the manufacturer or by the user according to the needs.

[0053] (I) If the cleaning device is debugged in advance by the manufacturer, the user can directly use it without calibrating the values ​​A1 and A2. At this time, the user operates the cleaning device in the following steps:

[0054] First, open the solenoid valve G31, and introduce the suspension containing microcarriers into the cleaning tank 1 through the liquid inlet pipe C19 until the value of the electronic scale 7 reaches A1, then close the solenoid valve G31, and then open the solenoid valve D26 and the solenoid valve E27, and discharge all the suspension containing no microcarriers in the cleaning tank 1 into the waste liquid bucket 3 through the liquid outlet pipe B18, and then perform subsequent operations according to the fourth to eighth steps mentioned above.

[0055] (ii) If the user debugs by himself, the user can debug according to the first and second steps above. At this time, when the user operates the cleaning device, the specific operating steps are the same as the first to eighth steps above.

[0056] Finally, it should be noted that the above description is only an embodiment of the present utility model, and does not limit the patent scope of the present utility model. Any equivalent structure or equivalent process transformation made using the contents of the present utility model specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present utility model.

Claims

1. A fully automatic microcarrier cleaning device, characterized in that: The invention comprises a cleaning tank (1), a liquid storage barrel (2), a waste liquid barrel (3), a collecting bottle (4), a magnetic stirrer (6), an electronic scale (7) and a controller, wherein the magnetic stirrer (6) is arranged on the top of the tank body of the cleaning tank (1), the cleaning tank (1) is arranged on the upper part of the electronic scale (7), a silicone tube joint (8) is connected to the lower part of one side of the tank body of the cleaning tank (1), and the silicone tube joint (8) and the tank bottom of the cleaning tank (1) are at the same height, the silicone tube joint (8) is connected to an inlet and outlet liquid pipe (12), the inlet and outlet liquid pipe (12) is connected to a liquid inlet pipe A (14) and a liquid outlet pipe A (15) through a three-way pipe joint (13), the liquid inlet pipe A (14) is connected to the liquid storage barrel (2), the liquid outlet pipe A (15) is connected to the collecting bottle (4), the liquid storage barrel (2) is also connected to a liquid inlet pipe B (16), and the liquid inlet pipe B (16) is connected to the three-way pipe joint (13). An annular liquid spray pipe (17) is provided, and the annular liquid spray pipe (17) is suspended inside the tank body of the cleaning tank (1) near the upper end, and is sleeved on the outside of the stirring shaft of the magnetic stirrer (6). At least one liquid outlet pipe B (18) is connected to the bottom of the tank body of the cleaning tank (1), and the other end of the at least one liquid outlet pipe B (18) is communicated with the waste liquid bucket (3). A filter screen (11) is also provided on the bottom wall of the tank body of the cleaning tank (1). Solenoid valves A (23), B (24), C (25) and D (26) are respectively provided on the liquid inlet pipe A (14), the liquid outlet pipe A (15), the liquid inlet pipe B (16) and the liquid outlet pipe B (18), and the solenoid valves A (23), B (24), C (25) and D (26) are all electrically connected to a controller, and the controller is also electrically connected to the magnetic stirrer (6) and the electronic scale (7).

2. The fully automatic microcarrier cleaning device according to claim 1, characterized in that: A liquid inlet pipe C (19) is also connected to the top of the tank body of the cleaning tank (1), and the other end of the liquid inlet pipe C (19) is connected to the microcarrier container (5) for inputting the microcarrier suspension in the microcarrier container (5) into the cleaning tank (1).

3. The fully automatic microcarrier cleaning device according to claim 2, characterized in that: An air intake pipe A (20) is also connected to the top of the tank body of the cleaning tank (1), and an electromagnetic valve E (27) and an air filter A (28) are arranged on the air intake pipe A (20), and the electromagnetic valve E (27) is electrically connected to the controller.

4. The fully automatic microcarrier cleaning device according to claim 1, characterized in that: The liquid storage barrel (2) is also connected to an air intake pipe B (21), on which an electromagnetic valve F (29) and an air filter B (30) are arranged, and the electromagnetic valve F (29) is electrically connected to the controller.

5. The fully automatic microcarrier cleaning device according to claim 2, characterized in that: The microcarrier container (5) is also connected to an air intake pipe C (22), on which an electromagnetic valve G (31) and an air filter C (32) are arranged, and the electromagnetic valve G (31) is electrically connected to the controller.

6. The fully automatic microcarrier cleaning device according to claim 1, characterized in that: An air filter D (33) is also provided on the top of the cleaning tank (1), the waste liquid bucket (3) and the collecting bottle (4).

7. The fully automatic microcarrier cleaning device according to claim 1, characterized in that: A low liquid level line (10) is arranged on the outer wall of the cleaning tank (1), and the position of the low liquid level line (10) is slightly higher than the position of the stirring blade of the magnetic stirrer (6).

8. The fully automatic microcarrier cleaning device according to claim 7, characterized in that: A high liquid level line (9) is arranged on the outer wall of the cleaning tank (1), and the position of the high liquid level line (9) is lower than the position of the three-way pipe joint (13).

9. The fully automatic microcarrier cleaning device according to claim 1, characterized in that: The cleaning tank (1) is made of transparent glass.

Images