Equipment for extracting silk fibroin solution from silkworm cocoons

Abstract

The utility model discloses equipment for extracting a silk fibroin solution from silkworm cocoons, and relates to the technical field of silk fibroin extraction. Comprising a degumming tank, a cleaning and drying tank, a dissolving tank and an impurity removal dialysis tank which are arranged in sequence, screen frames used for containing silkworm cocoons are placed in the degumming tank, the cleaning and drying tank and the dissolving tank. The mechanical arm is used for moving the screen frame; the water inlet end of the water pump is connected with the dissolving tank, and the water outlet end of the water pump is connected with the impurity removal dialysis tank. The steps of degumming, cleaning and drying, dissolving, impurity removal and dialysis are integrated, the whole process of extracting a silk fibroin solution from silkworm cocoons is realized, the sequence is changed through the mechanical arm, the whole process adopts automatic operation, the extraction process is simple, treatment is performed in different procedures, and the extraction efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of silk fibroin extraction, in particular to a device for extracting silk fibroin solution from silkworm cocoons. Background Art

[0002] Silk is divided into sericin and fibroin, of which fibroin is the main component, accounting for about 70-80%. Due to its excellent biocompatibility, non-immunogenicity, and controllable degradation in vivo, the degradation product is safe and non-toxic, and it is becoming a new type of medical biomaterial. It can be used as a product directly for tissue repair in clinical practice, and can also be used as a carrier scaffold for drugs and cells for sustained drug release and tissue engineering to construct artificial organs. Fibroin solution is a light yellow clear protein aqueous solution obtained by a series of purification processes after the silk fiber after the sericin is removed. Fibroin solution is the basis for preparing fibroin molding materials.

[0003] In the prior art, silk fibroin solution is mostly extracted manually using simple boiling tools, which requires multiple transfers, and the extraction process is cumbersome and the extraction efficiency is low. A small number of extraction devices have also appeared, such as a silk fibroin preparation device disclosed in a Chinese patent with publication number CN216427174U, which integrates the steps of cleaning, dissolving, and dialysis, but it does not use silkworm cocoons as raw materials, lacks degumming and impurity removal processes, and still needs to be improved. Utility Model Content

[0004] The main purpose of the utility model is to provide a device for extracting silk fibroin solution from silk cocoons to solve the above problems.

[0005] To achieve the above-mentioned purpose, the utility model provides a device for extracting silk fibroin solution from silk cocoons, comprising a degumming tank, a cleaning and drying tank, a dissolving tank and an impurity removal dialysis tank arranged in sequence; a net frame for holding silk cocoons is placed in the degumming tank, the cleaning and drying tank and the dissolving tank; and a mechanical arm for moving the net frame is also included;

[0006] The top of the side wall of the degumming tank is provided with a degumming liquid inlet, the bottom of the side wall is provided with a degumming waste liquid outlet, and the inner bottom of the degumming tank is provided with a first heating tube;

[0007] The top of the side wall of the cleaning and drying tank is provided with a pure water inlet, the bottom of the side wall is provided with a cleaning waste liquid outlet, the side wall of the cleaning and drying tank is provided with a heating fan, and the inner bottom of the cleaning and drying tank is provided with a plurality of blowing pipes connected to the air outlet of the heating fan;

[0008] The top of the side wall of the dissolving tank is provided with a dissolving liquid inlet, the bottom of the side wall is provided with a solution outlet, and the inner bottom of the dissolving tank is provided with a second heating pipe;

[0009] The impurity filter screen and the dialysis membrane are arranged in sequence from top to bottom inside the impurity removal dialysis tank, and the impurity filter screen and the dialysis membrane divide the interior of the impurity removal dialysis tank into a liquid inlet cavity, a dialysis cavity and a waste liquid cavity from top to bottom in sequence; the side wall of the impurity removal dialysis tank is provided with a solution inlet connected to the liquid inlet cavity, a finished product outlet connected to the dialysis cavity and a dialysis waste liquid outlet connected to the waste liquid cavity;

[0010] It also includes a water pump, wherein the water inlet end of the water pump is connected to the solution outlet, and the water outlet end of the water pump is connected to the solution inlet.

[0011] Furthermore, the tops of the degumming tank, the cleaning and drying tank, the dissolving tank and the impurity removal dialysis tank are all provided with tank covers that can be opened and closed automatically.

[0012] Furthermore, the tank covers of the degumming tank, the cleaning and drying tank and the dissolving tank are all provided with exhaust holes, and the exhaust holes are connected to purifiers.

[0013] Furthermore, the degumming tank, the cleaning and drying tank and the dissolving tank are all provided with temperature sensors.

[0014] Furthermore, the robotic arm includes a gantry, a transverse moving mechanism is provided on the gantry, a longitudinal moving mechanism is provided on the transverse moving mechanism, and a clamp is provided at the lower end of the longitudinal moving mechanism.

[0015] Furthermore, the dialysis membrane is arranged to be inclined toward the finished product outlet, and a stirring roller is arranged in the dialysis cavity.

[0016] The utility model has the following beneficial effects:

[0017] The utility model integrates the steps of degumming, cleaning and drying, dissolving, impurity removal and dialysis, realizes the whole process of extracting silk fibroin solution from silk cocoons, and performs the sequence transfer through a mechanical arm. The whole process is fully automated, the extraction process is simple, and the process is divided into steps, thereby improving the extraction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The utility model is a schematic diagram of a device for extracting silk fibroin solution from silk cocoons.

[0019] In the figure: 1-mechanical arm; 2-degumming tank; 3-cleaning and drying tank; 4-dissolving tank; 5-impurity removal dialysis tank; 6-water pump; 7-tank cover; 8-temperature sensor; 9-net frame; 21-degumming liquid inlet; 22-degumming waste liquid outlet; 23-first heating tube; 31-pure water inlet; 32-cleaning waste liquid outlet; 33-air blowing pipe; 34-heating fan; 41-dissolving liquid inlet; 42-solution outlet; 43-second heating tube; 51-solution inlet; 52-dialysis waste liquid outlet; 53-finished product outlet; 54-impurity filter; 55-stirring roller; 56-dialysis membrane; 71-exhaust hole; 72-purifier. DETAILED DESCRIPTION

[0020] In order to achieve the above-mentioned purpose and effect, the technical means and structure adopted by the utility model are described in detail with reference to the accompanying drawings for the preferred embodiments of the utility model and their features and functions.

[0021] As attached Figure 1 As shown, the utility model provides a device for extracting silk fibroin solution from silk cocoons, comprising a degumming tank 2, a cleaning and drying tank 3, a dissolving tank 4 and a decontamination dialysis tank 5 arranged in sequence; a net frame 9 for holding silk cocoons is placed in the degumming tank 2, the cleaning and drying tank 3 and the dissolving tank 4; a temperature sensor 8 and a liquid level sensor are arranged in the degumming tank 2, the cleaning and drying tank 3 and the dissolving tank 4, and also comprises a mechanical arm 1 for moving the net frame 9;

[0022] The top of the side wall of the degumming tank 2 is provided with a degumming liquid inlet 21, the bottom of the side wall is provided with a degumming waste liquid outlet 22, and the inner bottom of the degumming tank 2 is provided with a first heating pipe 23;

[0023] The top of the side wall of the cleaning and drying tank 3 is provided with a pure water inlet 31, the bottom of the side wall is provided with a cleaning waste liquid outlet 32, the side wall of the cleaning and drying tank 3 is provided with a heating fan 34, and the inner bottom of the cleaning and drying tank 3 is provided with a plurality of blowing pipes 33 connected with the air outlet of the heating fan 34;

[0024] The top of the side wall of the dissolving tank 4 is provided with a solution inlet 41, the bottom of the side wall is provided with a solution outlet 42, and the inner bottom of the dissolving tank 4 is provided with a second heating pipe 43;

[0025] The impurity filter screen 54 and the dialysis membrane 56 are arranged in sequence from top to bottom inside the impurity removal dialysis tank 5, and the impurity filter screen 54 and the dialysis membrane 56 divide the interior of the impurity removal dialysis tank 5 into a liquid inlet cavity, a dialysis cavity and a waste liquid cavity from top to bottom; the side wall of the impurity removal dialysis tank is provided with a solution inlet 51 connected to the liquid inlet cavity, a finished product outlet 53 connected to the dialysis cavity and a dialysis waste liquid outlet 52 connected to the waste liquid cavity;

[0026] The water pump 6 is also included. The water inlet end of the water pump 6 is connected to the solution outlet 42 , and the water outlet end of the water pump 6 is connected to the solution inlet 51 .

[0027] When in use, the silkworm cocoons are placed in an empty mesh frame 9, and then the robot arm 1 automatically extracts the mesh frame 9, puts the mesh frame 9 into the degumming tank 2, opens the degumming liquid inlet 21, and the degumming liquid enters the degumming tank 2. After reaching the specified liquid level, the degumming liquid inlet 21 is closed, and the first heating tube 23 heats the degumming liquid in the degumming tank 2 to a specified temperature, boils and degummes the silkworm cocoons in the mesh frame 9, and after the specified time, opens the degumming waste liquid outlet 22 to discharge the degumming waste liquid, closes it after the drainage is completed, and then the robot arm 1 puts the degumming tank 2 is extracted and placed in the cleaning and drying tank 3, the pure water inlet 31 is opened, and the pure water enters the cleaning and drying tank 3. After reaching the specified liquid level, the pure water inlet 31 is closed, the heating fan 34 is turned on, and the air blowing pipe 33 blows air to drive the pure water to roll upward to clean the silk cocoons in the net frame 9. After the specified time, the cleaning waste liquid outlet 32 ​​is opened to discharge the cleaning waste liquid, and the heating fan 34 is not stopped at the same time. The air blowing pipe 33 continues to blow out hot air to dry the silk cocoons in the net frame 9. After the specified time, , turn off the heating fan 34 and the cleaning waste liquid outlet 32, then the robot arm 1 extracts the mesh frame 9 in the cleaning and drying tank 3 and puts it into the dissolving tank 4, opens the dissolving liquid inlet 41, and the dissolving liquid enters the dissolving tank 4. After reaching the specified liquid level, the dissolving liquid inlet 41 is closed, and the second heating tube 43 heats the dissolving liquid in the dissolving tank 4 to a specified temperature to dissolve the cocoons in the mesh frame 9. After the specified time, the solution outlet 42, the water pump 6 and the solution inlet 51 are opened to discharge the solution into the impurity removal dialysis tank 5. After the discharge is completed, the solution outlet 42, the water pump 6 and the solution inlet 51 are closed, and then the robot arm 1 takes out the empty mesh frame 9 after the dissolution is completed from the dissolving tank 4, and new cocoons can be added; the solution entering the impurity removal dialysis tank 5 is filtered out of insoluble particulate impurities through the impurity filter 54, and then the small molecule solvent is removed through the dialysis membrane 56. At the same time, the dialysis waste liquid outlet 52 is opened to discharge the dialysis waste liquid. After dialysis for a certain period of time, the finished product outlet 53 is opened to obtain the purified silk fibroin solution.

[0028] The utility model integrates the steps of degumming, cleaning and drying, dissolving, impurity removal and dialysis, realizes the whole process of extracting silk fibroin solution from silk cocoons, and performs the sequence transfer through a mechanical arm. The whole process is fully automated, the extraction process is simple, and the process is divided into steps, thereby improving the extraction efficiency.

[0029] In another embodiment, the tops of the degumming tank 2, the cleaning and drying tank 3, the dissolving tank 4 and the impurity removal dialysis tank 5 are all provided with tank covers 7 that can be opened and closed automatically to prevent steam leakage; preferably, the tank covers 7 of the degumming tank 2, the cleaning and drying tank 3 and the dissolving tank 4 are all provided with exhaust holes 71, and the exhaust holes are connected to a purifier 72 to prevent steam from polluting the external environment while ensuring pressure relief.

[0030] In another embodiment, the mechanical arm 1 includes a gantry 11, a lateral moving mechanism 12 is provided on the gantry 11, a longitudinal moving mechanism 13 is provided on the lateral moving mechanism 12, a clamping claw 14 is provided at the lower end of the longitudinal moving mechanism 13, and the lateral moving mechanism 12 and the longitudinal moving mechanism 13 can adopt a servo slide, an electric cylinder or a pneumatic cylinder. A support rod is provided on the upper part of the screen frame 9, and the clamping claw 14 can clamp the support rod to extract the screen frame 9.

[0031] In another embodiment, the dialysis membrane 56 is tilted toward the finished product outlet 53 to facilitate the outflow of the finished product solution; a stirring roller 55 is provided in the dialysis chamber, and the solution on the side of the finished product outlet 53 can be moved to the other side by the counterclockwise stirring of the stirring roller 55, and the impurity filter 54 and the dialysis membrane 56 are continuously flushed, which can prevent the impurity filter 54 and the dialysis membrane 56 from being blocked on the one hand, and can speed up the dialysis process and shorten the dialysis time on the other hand. A mesh plate fixedly connected to the impurity removal dialysis tank 5 is provided below the dialysis membrane 56 to support the dialysis membrane 56, and an annular pressure plate detachably connected to the mesh plate is provided above the dialysis membrane 56 to fix the dialysis membrane 56. The detachable form facilitates the replacement of the dialysis membrane 56.

[0032] The above description is only the preferred embodiment of the utility model, not all embodiments. Anyone should be aware that structural changes made under the inspiration of the utility model, and all technical solutions that are the same or similar to the utility model, belong to the protection scope of the utility model.

Claims

1. A device for extracting silk fibroin solution from silkworm cocoons, characterized in that: It includes a degumming tank, a cleaning and drying tank, a dissolving tank and an impurity removal dialysis tank arranged in sequence; a net frame for holding silk cocoons is placed in each of the degumming tank, the cleaning and drying tank and the dissolving tank; and it also includes a mechanical arm for moving the net frame; The top of the side wall of the degumming tank is provided with a degumming liquid inlet, the bottom of the side wall is provided with a degumming waste liquid outlet, and the inner bottom of the degumming tank is provided with a first heating tube; The top of the side wall of the cleaning and drying tank is provided with a pure water inlet, the bottom of the side wall is provided with a cleaning waste liquid outlet, the side wall of the cleaning and drying tank is provided with a heating fan, and the inner bottom of the cleaning and drying tank is provided with a plurality of blowing pipes connected to the air outlet of the heating fan; The top of the side wall of the dissolving tank is provided with a dissolving liquid inlet, the bottom of the side wall is provided with a solution outlet, and the inner bottom of the dissolving tank is provided with a second heating pipe; The impurity filter screen and the dialysis membrane are arranged in sequence from top to bottom inside the impurity removal dialysis tank, and the impurity filter screen and the dialysis membrane divide the interior of the impurity removal dialysis tank into a liquid inlet cavity, a dialysis cavity and a waste liquid cavity from top to bottom in sequence; the side wall of the impurity removal dialysis tank is provided with a solution inlet connected to the liquid inlet cavity, a finished product outlet connected to the dialysis cavity and a dialysis waste liquid outlet connected to the waste liquid cavity; It also includes a water pump, wherein the water inlet end of the water pump is connected to the solution outlet, and the water outlet end of the water pump is connected to the solution inlet.

2. The device for extracting silk fibroin solution from silk cocoons as claimed in claim 1, characterized in that: The tops of the degumming tank, the cleaning and drying tank, the dissolving tank and the impurity removal dialysis tank are all provided with tank covers which can be opened and closed automatically.

3. The device for extracting silk fibroin solution from silk cocoons as claimed in claim 2, characterized in that: The tank covers of the degumming tank, the cleaning and drying tank and the dissolving tank are all provided with exhaust holes, and the exhaust holes are connected with purifiers.

4. The device for extracting silk fibroin solution from silk cocoons as claimed in claim 1, characterized in that: The degumming tank, the cleaning and drying tank and the dissolving tank are all provided with temperature sensors.

5. The device for extracting silk fibroin solution from silk cocoons as claimed in claim 1, characterized in that: The mechanical arm comprises a gantry, a transverse moving mechanism is arranged on the gantry, a longitudinal moving mechanism is arranged on the transverse moving mechanism, and a clamping claw is arranged at the lower end of the longitudinal moving mechanism.

6. The device for extracting silk fibroin solution from silk cocoons as claimed in claim 1, characterized in that: The dialysis membrane is arranged obliquely toward the finished product outlet, and a stirring roller is arranged in the dialysis cavity.

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