Liquid adding device for fecal coliform culture based on multi-tube fermentation method

Abstract

The utility model discloses a liquid adding device for fecal coliform culture based on a multi-tube fermentation method, and relates to the technical field of flora culture. The colorimetric tube racks are square tube racks, three rows of five-hole test tube openings are formed in the colorimetric tube racks, the colorimetric tube racks are movably arranged on the top of the machine table side by side, a positioning table matched with the colorimetric tube racks is arranged on the front side of the top of the machine table, a side bin is fixed on the rear side of the top of the machine table, and side seats are arranged on the two sides of the side bin; a front transverse plate is fixed to the upper portion between the ends, away from the side bins, of the two side bases, and a receding area is formed between the front transverse plate and the machine table. According to the colorimetric tube rack liquid adding device, a plurality of colorimetric tube racks can be positioned and arranged through the designed novel liquid adding device, the quantitative gun heads can be sequentially opposite to the test tubes below through the slidable longitudinal beams and the flow dividing tubes, liquid can be quantitatively added into the test tubes through the quantitative gun heads, the liquid adding efficiency and precision can be greatly improved, and therefore the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of bacterial colony cultivation, in particular to a liquid adding device for fecal coliform colony cultivation based on a multi-tube fermentation method. Background Art

[0002] For the determination of fecal coliform group in water quality, the 15-tube method in the multiple-tube fermentation method is mostly used. In the initial fermentation test, after the sample is fully mixed, 10 ml of the sample is added to 5 test tubes containing 5 ml of sterilized culture medium (with inverted tubes inside) according to the requirements of sterile operation, 1 ml of the sample is added to 5 test tubes containing 10 ml of sterilized culture medium (with inverted tubes inside) according to the requirements of sterile operation, and 0.1 ml of the sample is added to 5 test tubes containing 10 ml of sterilized culture medium (with inverted tubes inside) according to the requirements of sterile operation. The inoculated test tubes are then cultured at 37℃±0.5℃ for 24h±2h. The color of the fermentation test tube turns yellow for acid production, and bubbles are present in the small glass inverted tube for gas production. Test tubes that produce acid and gas indicate a positive test.

[0003] In the actual experimental process, fifteen fermentation test tubes are used for a single experiment. At the same time, multiple water quality samples need to be cultured simultaneously during one experiment. The amount used is too large, and liquid needs to be added to each test tube, which makes the experimental process cumbersome and time-consuming. Although manual liquid addition can be accurate, the work efficiency is too low. Therefore, designing an automated and precisely controlled liquid addition device is a problem that needs to be solved by the staff in this field. Summary of the invention

[0004] The utility model aims to provide a liquid adding device for culturing fecal coliform group based on a multi-tube fermentation method. By designing a novel liquid adding device, a plurality of colorimetric tube racks can be positioned and arranged. The quantitative gun head can be aligned with the test tubes below in turn through a slidable longitudinal beam and a shunt tube. Liquid can be added to the test tubes quantitatively through the quantitative gun head, which can greatly improve the liquid adding efficiency and accuracy, thereby improving the experimental efficiency.

[0005] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0006] The utility model is a liquid adding device for culturing fecal coliform bacteria based on a multi-tube fermentation method, comprising a machine platform and a plurality of colorimetric tube racks;

[0007] The colorimetric tube rack is a square tube rack, and is provided with three rows of five-hole test tube ports. A plurality of the colorimetric tube racks are movably arranged side by side on the top of the machine, and a positioning platform cooperating with the colorimetric tube rack is provided on the front side of the top of the machine;

[0008] A side bin is fixed to the rear side of the top of the machine, and side seats are provided on both sides of the side bin. A front horizontal plate is fixed to the upper part between the two side seats away from one end of the side bin, and a clearance area is formed between the front horizontal plate and the machine;

[0009] An electric transverse slide is provided in the side bin, a slide seat is slidably connected to the electric transverse slide, a longitudinal beam is fixed to the front side of the slide seat through a connecting block, and one end of the longitudinal beam away from the slide seat is slidably connected to the inner side of the front transverse plate;

[0010] A shunt tube is fixed on the longitudinal beam or three shunt tubes are fixed via a connecting frame, and five quantitative gun heads are connected to the bottom of the shunt tube, and the spacing between the five quantitative gun heads is consistent with the spacing between the five test tube openings in each row on the colorimetric tube rack;

[0011] The middle part of the rear side of the side bin is movably connected to a culture liquid tank body through a support seat, a syringe pump is fixed on the top of the connection block, the end of the shunt pipe is connected to the syringe pump outlet, and the syringe pump inlet is connected to the culture liquid tank body through a long hose.

[0012] Furthermore, the colorimetric tube rack includes a group of side panels, a frame plate is provided at the upper and middle parts between the group of side panels, the test tube port is arranged on the frame plate, a bottom plate is fixed at the lower part between the group of side panels, and a positioning groove cooperating with the positioning platform is formed between the bottom of the bottom plate and the bottom edges of the two side panels.

[0013] Furthermore, three positioning protrusions are provided on the top surface of the shelf plate above the colorimetric tube rack near one end of the side bin, and the three positioning protrusions are respectively arranged on one side of the three rows of test tube openings on the shelf plate, and a proximity sensor cooperating with the positioning protrusions is provided at the bottom of the connecting block.

[0014] Furthermore, a positioning ring is fixed on the top of the support seat, and the gap at the bottom of the culture liquid tank is arranged in the positioning ring.

[0015] Furthermore, a group of slide grooves are provided on the inner side of the front transverse plate, and a group of guide wheels are provided at the end of the longitudinal beam, and the two guide wheels roll along the two slide grooves respectively.

[0016] Furthermore, shields are fixed between the two sides of the slide seat and the two sides in the side bin, and the shields are I-shaped accordion shields or U-shaped accordion shields.

[0017] The utility model has the following beneficial effects:

[0018] The utility model can position and arrange a plurality of colorimetric tube racks by designing a novel liquid adding device, can sequentially align the quantitative gun head with the test tubes below by means of the slidable longitudinal beam and the diverter tube, can quantitatively add liquid to the test tubes by means of the quantitative gun head, can greatly improve the liquid adding efficiency and accuracy, and thus improve the experimental efficiency.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 It is a structural schematic diagram of embodiment 1;

[0022] Figure 2 for Figure 1 A partial enlarged view of the middle A;

[0023] Figure 3 It is a structural cross-sectional view of the connection between the longitudinal beam and the front transverse plate of the first embodiment;

[0024] Figure 4 It is a structural schematic diagram of a colorimetric tube rack;

[0025] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0026] 1-machine table, 2-colorimetric tube rack, 3-culture liquid tank, 4-shield, 101-side compartment, 102-side seat, 103-front horizontal plate, 104-slide seat, 105-connecting block, 106-longitudinal beam, 107-diverter tube, 108-quantitative gun head, 109-support seat, 110-syringe pump, 111-long hose, 112-positioning table, 113-positioning ring, 114-slide, 115-guide wheel, 201-test tube mouth, 202-side plate, 203-rack plate, 204-bottom plate, 205-positioning protrusion. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Example

[0028] See also Figure 1-4 As shown, the utility model is a liquid adding device for fecal coliform culture based on a multi-tube fermentation method, comprising a machine 1 and a plurality of colorimetric tube racks 2;

[0029] The colorimetric tube rack 2 is a square tube rack, and is provided with three rows of five-hole test tube ports 201. A plurality of colorimetric tube racks 2 are movably arranged side by side on the top of the machine 1, and a positioning table 112 cooperating with the colorimetric tube rack 2 is provided on the front side of the top of the machine 1;

[0030] A side bin 101 is fixed to the rear side of the top of the machine 1, and side seats 102 are provided on both sides of the side bin 101. A front horizontal plate 103 is fixed to the upper part between the two side seats 102 away from one end of the side bin 101, and a clearance area is formed between the front horizontal plate 103 and the machine 1;

[0031] An electric horizontal slide is provided in the side compartment 101, and a slide seat 104 is slidably connected to the electric horizontal slide. A longitudinal beam 106 is fixed to the front side of the slide seat 104 through a connecting block 105. The longitudinal beam 106 is slidably connected to the inner side of the front horizontal plate 103 at one end away from the slide seat 104.

[0032] A shunt tube (107) is fixed on the longitudinal beam 106, and five quantitative gun tips 108 are connected to the bottom of the shunt tube 107. The spacing between the five quantitative gun tips 108 is consistent with the spacing between the five test tube openings 201 in each row on the colorimetric tube rack 2.

[0033] The middle part of the rear side of the side bin 101 is movably connected to the culture liquid tank body 3 through the support seat 109, the top of the connecting block 105 is fixed with an injection pump 110, the end of the shunt tube 107 is connected to the outlet of the injection pump 110, and the inlet of the injection pump 110 is connected to the culture liquid tank body 3 through a long hose 111.

[0034] Among them Figure 1-4 As shown, the colorimetric tube rack 2 includes a group of side panels 202, a frame plate 203 is provided at the upper and middle parts between the group of side panels 202, the test tube port 201 is arranged on the frame plate 203, a bottom plate 204 is fixed at the lower part between the group of side panels 202, and a positioning groove cooperating with the positioning platform 112 is formed between the bottom of the bottom plate 204 and the bottom edges of the two side panels 202.

[0035] Among them Figure 4 As shown, three positioning protrusions 205 are provided on the top surface of the shelf plate 203 above the colorimetric tube rack 2 near one end of the side compartment 101. The three positioning protrusions 205 are respectively arranged on one side of the three rows of test tube ports 201 on the shelf plate 203, and a proximity sensor cooperating with the positioning protrusions 205 is provided at the bottom of the connecting block 105.

[0036] Among them Figure 1 As shown, a positioning ring 113 is fixed on the top of the support seat 109 , and the gap at the bottom of the culture liquid tank body 3 is set in the positioning ring 113 .

[0037] Among them Figure 3As shown, a group of slide grooves 114 are provided on the inner side of the front cross plate 103 , and a group of guide wheels 115 are provided on the end of the longitudinal beam 106 , and the two guide wheels 115 roll along the two slide grooves 114 respectively.

[0038] Among them Figure 1-2 As shown, shields 4 are fixed between the two sides of the slide seat 104 and the two sides inside the side bin 101 , and the shields 4 are straight-line accordion shields or U-shaped accordion shields.

[0039] The working principle of this embodiment is as follows: a test tube with an inverted tube inside is placed in the test tube opening 201 of the colorimetric tube rack 2. After all the colorimetric tube racks 2 are filled, they are transferred to the machine table 1. The slide seat 104 can be started to slide horizontally by an electric horizontal slide. The longitudinal beam 106 and the shunt tube 107 can be correspondingly moved to the top of the test tube opening 201 on the side of the colorimetric tube rack 2 by the cooperation of the positioning protrusion 205 and the proximity sensor at the bottom of the connecting block 105. The culture solution in the culture solution tank 3 can be transferred to the shunt tube 107 by the injection pump 110 and the hose 111, and the culture solution is added to the test tube below by five quantitative gun heads 108. After the culture solution in the first row of test tubes is filled, the shunt tube 107 is moved to the top of the next row of test tubes by the electric horizontal slide, and 10 ml of culture solution is injected into the three rows of test tubes on the colorimetric tube rack 2. Example

[0040] The difference between this embodiment and the first embodiment is that three shunt pipes 107 are fixed on the longitudinal beam 106 via a connecting frame.

[0041] There are five quantitative gun tips 108 at the bottom of each shunt tube 107 , and the distribution intervals and arrangement of the fifteen quantitative gun tips 108 are consistent with the distribution intervals and arrangement of the fifteen test tube openings 201 on the colorimetric tube rack 2 .

[0042] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0043] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A liquid adding device for culturing fecal coliform bacteria based on a multi-tube fermentation method, comprising a machine (1) and a plurality of colorimetric tube racks (2), characterized in that: The colorimetric tube rack (2) is a square tube rack, and is provided with three rows of five-hole test tube openings (201). A plurality of the colorimetric tube racks (2) are movably arranged side by side on the top of the machine platform (1), and a positioning table (112) cooperating with the colorimetric tube rack (2) is provided on the front side of the top of the machine platform (1); A side bin (101) is fixed to the rear side of the top of the machine (1), side seats (102) are provided on both sides of the side bin (101), a front transverse plate (103) is fixed to the upper part between the two ends of the side seats (102) away from the side bin (101), and a clearance area is formed between the front transverse plate (103) and the machine (1); An electric transverse slide is provided in the side bin (101), a slide seat (104) is slidably connected to the electric transverse slide, a longitudinal beam (106) is fixed to the front side of the slide seat (104) via a connecting block (105), and an end of the longitudinal beam (106) away from the slide seat (104) is slidably connected to the inner side of the front transverse plate (103); A shunt tube (107) is fixed on the longitudinal beam (106) or three shunt tubes (107) are fixed via a connecting frame, and five quantitative gun tips (108) are connected to the bottom of the shunt tube (107), and the spacing between two of the five quantitative gun tips (108) is consistent with the spacing between two of the five test tube openings (201) in each row on the colorimetric tube rack (2); The middle part of the rear side of the side bin (101) is movably connected to a culture liquid tank body (3) via a support seat (109); a syringe pump (110) is fixed to the top of the connection block (105); the end of the shunt tube (107) is connected to the outlet of the syringe pump (110); and the inlet of the syringe pump (110) is connected to the culture liquid tank body (3) via a long hose (111).

2. A liquid adding device for culturing fecal coliform bacteria based on a multi-tube fermentation method according to claim 1, characterized in that: The colorimetric tube rack (2) comprises a group of side panels (202), a frame plate (203) is provided at the upper and middle parts between the group of side panels (202), the test tube opening (201) is arranged on the frame plate (203), a bottom plate (204) is fixed at the lower part between the group of side panels (202), and a positioning groove that cooperates with the positioning platform (112) is formed between the bottom of the bottom plate (204) and the bottom edges of the two side panels (202).

3. A liquid adding device for culturing fecal coliform bacteria based on a multi-tube fermentation method according to claim 2, characterized in that: Three positioning protrusions (205) are provided on the top surface of the shelf plate (203) above the colorimetric tube rack (2) near one end of the side compartment (101); the three positioning protrusions (205) are respectively arranged on one side of three rows of test tube openings (201) on the shelf plate (203); and a proximity sensor cooperating with the positioning protrusions (205) is provided at the bottom of the connecting block (105).

4. The liquid adding device for culturing fecal coliform bacteria based on the multi-tube fermentation method according to claim 1, characterized in that: A positioning ring (113) is fixed on the top of the support seat (109), and a gap at the bottom of the culture liquid tank body (3) is arranged in the positioning ring (113).

5. The liquid adding device for culturing fecal coliform bacteria based on the multi-tube fermentation method according to claim 1, characterized in that: A group of slide grooves (114) are provided on the inner side of the front transverse plate (103), and a group of guide wheels (115) are provided at the end of the longitudinal beam (106), and the two guide wheels (115) roll along the two slide grooves (114) respectively.

6. The liquid adding device for culturing fecal coliform bacteria based on the multi-tube fermentation method according to claim 1, characterized in that: A shield (4) is fixed between the two sides of the slide seat (104) and the two sides inside the side bin (101), and the shield (4) is a straight-shaped accordion shield or a U-shaped accordion shield.

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