Full-automatic gene sequencing library preparation instrument

By separating the lifting drive devices on opposite sides of the mounting plate and incorporating a rotating drive device, the fully automatic gene sequencing library preparation instrument achieves a more compact design, addressing the height and space issues of previous systems.

CN222250687UActive Publication Date: 2024-12-27MYGENOSTICS (CHONGQING) GENE TECH CO LTD
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Patent Information

Application Number
CN202420848761.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-23
Publication Date
2024-12-27
Estimated Expiration
2034-04-23

AI Technical Summary

Technical Problem

The existing fully automatic gene sequencing library preparation systems face an issue where the height of the frame increases due to the same-side installation of the first and second lifting drive mechanisms, leading to increased volume and space occupation, making the system inconvenient to use.

Method used

The first and second lifting drive devices are arranged on opposite sides of the mounting plate, with a rotating drive device in between, allowing for a more compact design that reduces the overall height and optimizes space utilization.

Benefits of technology

This configuration minimizes the height and volume of the instrument, enhancing usability by reducing space occupation and facilitating efficient operation of the reagent storage reaction box.

✦ Generated by Eureka AI based on patent content.

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    Figure CN222250687U_ABST
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Abstract

The utility model discloses a full-automatic gene sequencing library preparation instrument which comprises a rack, a first lifting seat, a first lifting driving device, a second lifting seat, a second lifting driving device, a rotating seat, a rotating driving device, a gun head driving device, a reagent storage reaction box and a temperature control supporting seat, a first mounting plate is arranged on the rack, a first lifting seat and a second lifting seat which can move in the Y direction are mounted on the first side of the first mounting plate, the first lifting seat is located above the second lifting seat and connected to the output end of a first lifting driving device, and the first lifting driving device is arranged beside the first side of the first mounting plate; the second side of the first mounting plate is opposite to the first side of the first mounting plate. According to the full-automatic gene sequencing library preparation instrument, the problem that the height of the whole rack is increased due to the fact that the first lifting driving mechanism and the second lifting driving mechanism are installed on the same side in the prior art is solved.
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Description

Technical Field

[0001] The utility model relates to equipment for gene sequencing, in particular to a fully automatic gene sequencing library preparation instrument. Background Art

[0002] The Chinese patent discloses a fully automatic gene sequencing library preparation system with application number: CN202311447236.5 and a preparation method based thereon, wherein the fully automatic gene sequencing library preparation system comprises: a frame, which is provided with a storage place; a gene sequencing reagent kit comprises: a bottom box, an outer cylinder, a pipette tip, a lifting spring and a hose; the bottom box is provided with at least two reagent chambers, the outer cylinder is hinged to the bottom box, a first center hole is formed at the center of the outer cylinder, a second center hole is formed at the center of the bottom box, the first center hole is connected to the second center hole, and all reagent chambers are surrounded by the second center hole; a pipette tip is installed on the outer cylinder, the pipette tip can be raised and lowered relative to the outer cylinder, a lifting spring is installed between the pipette tip and the outer cylinder, and the lifting spring is used to keep the pipette tip away from the detection chamber.

[0003] Although the fully automatic gene sequencing library preparation system can solve the problem in the prior art that the negative pressure in the pipette tip cannot meet the requirement due to the insufficient sealing between the pipette tip and the separated lower pressure head in the gene sequencing kit, the fully automatic gene sequencing library preparation system still has the disadvantage that the first lifting drive mechanism and the second lifting drive device are both arranged on the same side of the frame, that is, the first lifting drive mechanism and the second lifting drive device are installed on the same side, resulting in the problem of a relatively high height of the entire frame. Utility Model Content

[0004] The utility model provides a fully automatic gene sequencing library preparation instrument, which solves the problem in the prior art that the height of the entire frame increases due to the same-side installation of the first lifting drive mechanism and the second lifting drive device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] The utility model discloses a fully automatic gene sequencing library preparation instrument, comprising: a frame, a first lifting seat, a first lifting drive device, a second lifting seat, a second lifting drive device, a rotating seat, a rotating drive device, a gun head drive device, a reagent storage reaction box and a temperature control support seat; a first mounting plate is arranged on the frame, a first lifting seat and a second lifting seat that can move in the Y direction are mounted on a first side of the first mounting plate, the first lifting seat is located above the second lifting seat, the first lifting seat is connected to an output end of the first lifting drive device, and the first lifting drive device is next to a first side of the first mounting plate; the second side of the first mounting plate is connected to the first side of the first mounting plate Relatively speaking, a second lifting drive device is installed on the second side of the first mounting plate, and the output end of the second lifting drive device is connected to the second lifting seat; a rotatable rotating disk is installed on the second lifting seat, and the output end of the rotating disk is passed through the rotating disk by the rotating drive device, and the rotating disk can be moved in the Y direction by the rotating drive device relative to the rotating disk; a gun head drive device is installed at the output end of the rotating drive device, and the gun head drive device is used to drive the pipette gun head located in the reagent storage reaction box to move in the Y direction; a temperature control support seat is installed on the frame, and the temperature control support seat is located below the second lifting seat, and the temperature control support seat is used to place the reagent storage reaction box.

[0007] Preferably, a pushing device is installed on the frame, and the pushing device is used to drive the temperature control support seat to move in the X direction.

[0008] Preferably, the installation position of the pushing device is located beside the second side of the first installation plate.

[0009] Preferably, a clamping device is installed on the temperature control support seat, the installation position of the clamping device is arranged in parallel with the installation position of the pushing device, and the clamping device is located beside the pushing direction.

[0010] Preferably, the temperature control support device includes: a box-type seat, a temperature control execution device, a heat sink, a first fan, a second fan and a positioning seat; the box-type seat is a tubular structure, and a positioning seat is installed on the box-type seat, the positioning seat is used to place a detachable reagent storage reaction box, and the temperature control execution device is installed below the positioning seat. The temperature control execution device adopts a refrigeration plate type, one side of the temperature control execution device is in close contact with the positioning seat, and the other side of the temperature control execution device extends into the inner hole of the box-type seat; a heat sink is installed in the box-type seat below the temperature control execution device, a first fan is installed at one end of the box-type seat, and a second fan is installed at the other end of the box-type seat, and the first fan and the second fan draw outside air into the box-type seat and then discharge it.

[0011] Preferably, an ultrasonic transducer is installed in the box-type seat, and the output end of the ultrasonic transducer faces the reagent storage reaction box located on the positioning seat.

[0012] Preferably, the second lifting drive device and the rotation drive device are both mounted on a second mounting plate, and the second mounting plate is mounted beside the first side of the first mounting plate.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] In the present application, the first lifting drive device and the second lifting drive device are respectively arranged beside the first side and the second side which are arranged relatively in the first mounting plate. Since the first lifting drive device and the second lifting drive device have their own height, when the first lifting drive device and the second lifting drive device are arranged on the same side, the height of the entire frame will be increased, resulting in an increase in the height of the entire fully automatic gene sequencing library preparation instrument, that is, an increase in the volume of the entire fully automatic gene sequencing library preparation instrument, which increases the occupied space and is inconvenient to use. However, in the present application, the first lifting drive device and the second lifting drive device are separately arranged on the first side and the second side which are opposite to each other in the first mounting plate, thereby reducing the height of the entire fully automatic gene sequencing library preparation instrument and making it more convenient to use. When the rotating seat is driven to rotate, the rotary drive device is used, and the rotary drive device is simultaneously arranged beside the first lifting drive device. The first lifting drive device and the rotary drive device are arranged in parallel, further avoiding the first lifting drive device and the rotary drive device being arranged in a high and low staggered manner, which causes the height of the entire fully automatic gene sequencing library preparation instrument to increase, thereby further reducing the occupied space of the fully automatic gene sequencing library preparation instrument, making full use of the upper space of the reagent storage reaction box, and reducing the entire occupied space.

[0015] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a fully automatic gene sequencing library preparation instrument.

[0017] Figure 2 This is a schematic diagram of the structure of a fully automatic gene sequencing library preparation instrument.

[0018] Figure 3 It is a schematic diagram of the structure of the temperature control support seat.

[0019] Figure numerals: frame 1, first mounting plate 111, second mounting plate 112, first lifting seat 2, first lifting drive device 3, second lifting seat 4, second lifting drive device 5, rotating seat 6, rotating drive device 7, gun head drive device 8, reagent storage reaction box 9, temperature control support seat 10, box seat 101, temperature control execution device 102, first fan 104, second fan 105, positioning seat 106, pushing device 11, pressing device 12. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and functions of the present invention clearer and easier to understand, the present invention is further described below in conjunction with the accompanying drawings and specific implementation methods:

[0021] like Figures 1 to 3 As shown, the utility model discloses a fully automatic gene sequencing library preparation instrument, comprising: a frame, a first lifting seat, a first lifting drive device, a second lifting seat, a second lifting drive device, a rotating seat, a rotating drive device, a gun head drive device, a reagent storage reaction box and a temperature control support seat; a first mounting plate is arranged on the frame, a first lifting seat and a second lifting seat that can move in the Y direction are mounted on a first side of the first mounting plate, the first lifting seat is located above the second lifting seat, the first lifting seat is connected to an output end of the first lifting drive device, and the first lifting drive device is next to a first side of the first mounting plate; the second side of the first mounting plate is connected to a first side of the first mounting plate The first mounting plate is arranged on opposite sides, and a second lifting drive device is installed on the second side of the first mounting plate, and the output end of the second lifting drive device is connected to the second lifting seat; a rotating disk that can rotate is installed on the second lifting seat, and the output end of the rotating drive device passes through the rotating disk, and the rotating disk can be moved in the Y direction relative to the rotating disk by the rotating drive device; a gun head drive device is installed at the output end of the rotating drive device, and the gun head drive device is used to drive the liquid suction gun head located in the reagent storage reaction box to move in the Y direction; a temperature control support seat is installed on the frame, and the temperature control support seat is located below the second lifting seat, and the temperature control support seat is used to place the reagent storage reaction box. The first lifting drive device, the second lifting drive device and the gun head drive device are all electric cylinder structures, and linear drive is achieved through a lead screw.

[0022] A pushing device is installed on the frame, and the pushing device is used to drive the temperature control support seat to move in the X direction. Since the second lifting seat cannot be raised to a very high position, the pushing device is designed to make the reagent storage reaction box deviate from the second lifting seat, so that the reagent storage reaction box can be installed on the temperature control support seat, or the reagent storage reaction box can be separated from the temperature control support seat.

[0023] The installation position of the pushing device is located beside the second side of the first installation plate. The installation position of the pushing device deviates from the working position of the reagent storage reaction box, and the output end of the pushing device can pass through the working position of the reagent storage reaction box, thereby causing the reagent storage reaction box to deviate from the working position, thereby preventing the second lifting seat from blocking the placement and removal of the reagent storage reaction box.

[0024] A clamping device is installed on the temperature control support seat, and the installation position of the clamping device is arranged in parallel with the installation position of the pushing device, and the clamping device is located beside the pushing direction. After the installation position of the clamping device is arranged in parallel with the installation position of the pushing device, the space occupied by the clamping device and the pushing device in the X direction is reduced.

[0025] The temperature control support device includes: a box-type seat, a temperature control execution device, a heat sink, a first fan, a second fan and a positioning seat; the box-type seat is a tubular structure, and a positioning seat is installed on the box-type seat. The positioning seat is used to place a detachable reagent storage reaction box, and the temperature control execution device is installed below the positioning seat. The temperature control execution device adopts a refrigeration plate type, one side of the temperature control execution device is tightly attached to the positioning seat, and the other side of the temperature control execution device extends into the inner hole of the box-type seat; a heat sink located below the temperature control execution device is installed in the box-type seat, a first fan is installed at one end of the box-type seat, and a second fan is installed at the other end of the box-type seat, and the first fan and the second fan draw outside air into the box-type seat and then discharge it. In the present application, the reagent storage reaction box provides a reaction chamber, so that the entire reaction chamber can be arranged outside the box seat (equivalent to the reaction chamber shell in the prior art), breaking the traditional thinking (the thinking that the entire reagent storage reaction box needs to be placed in the box seat), and realizing the externalization of the entire reagent storage reaction box. The refrigeration plate-type temperature control execution device on the box seat can control the temperature of the bottom of the reagent storage reaction box located on the positioning seat, so as to reach the temperature of the gene sequencing library preparation, and ensure that the reaction can proceed normally. In the present application, the temperature control execution device cools the reagent storage reaction box, and at the same time, heat is generated below the temperature control execution device, and the heat is gathered in the box seat. The first fan and the second fan are used to dissipate the heat of the box seat, so as to achieve rapid transfer of heat and avoid affecting the realization of the refrigeration function. The layout of the temperature control seat for preparing the entire gene sequencing library is completely different, which is convenient for replacing the reagent storage reaction box. And the entire heat dissipation circulation channel is arranged in the box seat, and the first fan and the second fan are arranged at the end of the box seat, which can reduce the volume of the temperature control seat for preparing the entire gene sequencing library. Compared with water cooling, it does not require the use of water, but only the use of the atmosphere. It can be used anytime and anywhere, making it more convenient to use.

[0026] An ultrasonic transducer is installed in the box-type seat, and the output end of the ultrasonic transducer faces the reagent storage reaction box located on the positioning seat.

[0027] The second lifting drive device and the rotating drive device are both mounted on a second mounting plate, and the second mounting plate is mounted beside a first side of the first mounting plate.

[0028] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A fully automatic gene sequencing library preparation instrument, characterized in that: include: A frame, a first lifting seat, a first lifting drive device, a second lifting seat, a second lifting drive device, a rotating seat, a rotating drive device, a gun head drive device, a reagent storage reaction box, and a temperature control support seat; A first mounting plate is provided on the frame, a first lifting seat and a second lifting seat that can move in the Y direction are installed on a first side of the first mounting plate, the first lifting seat is located above the second lifting seat, the first lifting seat is connected to an output end of a first lifting drive device, and the first lifting drive device is next to a first side of the first mounting plate; The second side of the first mounting plate is arranged opposite to the first side of the first mounting plate, a second lifting drive device is installed on the second side of the first mounting plate, and an output end of the second lifting drive device is connected to the second lifting seat; A rotatable rotating disk is installed on the second lifting seat, and the output end of the rotating disk rotation driving device passes through the rotating disk, and the rotating disk can move in the Y direction relative to the rotating disk rotation driving device; A gun tip driving device is installed at the output end of the rotary driving device, and the gun tip driving device is used to drive the liquid pipetting gun tip located in the reagent storage reaction box to move in the Y direction; A temperature control support seat is installed on the rack, and the temperature control support seat is located below the second lifting seat. The temperature control support seat is used for placing a reagent storage reaction box.

2. The fully automatic gene sequencing library preparation instrument according to claim 1, characterized in that: A pushing device is installed on the frame, and the pushing device is used to drive the temperature control support seat to move in the X direction.

3. The fully automatic gene sequencing library preparation instrument according to claim 2, characterized in that: The installation position of the pushing device is located beside the second side of the first installation plate.

4. The fully automatic gene sequencing library preparation instrument according to claim 3, characterized in that: A clamping device is installed on the temperature control support seat. The installation position of the clamping device is arranged in parallel with the installation position of the pushing device, and the clamping device is located beside the pushing direction.

5. The fully automatic gene sequencing library preparation instrument according to claim 4, characterized in that: The temperature control support device includes: a box-type seat, a temperature control execution device, a heat sink, a first fan, a second fan and a positioning seat; the box-type seat is a tubular structure, and a positioning seat is installed on the box-type seat. The positioning seat is used to place a detachable reagent storage reaction box, and the temperature control execution device is installed below the positioning seat. The temperature control execution device adopts a refrigeration plate type, one side of the temperature control execution device is tightly attached to the positioning seat, and the other side of the temperature control execution device extends into the inner hole of the box-type seat; a heat sink located below the temperature control execution device is installed in the box-type seat, a first fan is installed at one end of the box-type seat, and a second fan is installed at the other end of the box-type seat, and the first fan and the second fan draw outside air into the box-type seat and then discharge it.

6. The fully automatic gene sequencing library preparation instrument according to claim 5, characterized in that: An ultrasonic transducer is installed in the box-type seat, and the output end of the ultrasonic transducer faces the reagent storage reaction box located on the positioning seat.

7. The fully automatic gene sequencing library preparation instrument according to any one of claims 1 to 6, characterized in that: The second lifting drive device and the rotating drive device are both mounted on a second mounting plate, and the second mounting plate is mounted beside a first side of the first mounting plate.