Gene sequencing library preparation temperature control seat

Abstract

The utility model discloses a temperature control seat for preparing a gene sequencing library. The temperature control seat comprises a box-type seat, a temperature control execution device, a cooling fin, a first fan, a second fan, a positioning seat and a clamping mechanism, the box-type seat is of a tubular structure, a positioning seat is installed on the box-type seat and used for placing a detachable reagent storage reaction box, a temperature control execution device is installed below the positioning seat and is in a refrigeration sheet type, one side of the temperature control execution device is tightly attached to the positioning seat, and the other side of the temperature control execution device extends into an inner hole of the box-type seat; a clamping mechanism is mounted on the box-type seat and is used for clamping the reagent storage reaction box positioned on the positioning seat in the horizontal direction. The temperature control seat for preparing the gene sequencing library solves the problem that in the prior art, a small reaction chamber cannot be installed outside a temperature-controlled reaction bin shell.

Description

Technical Field

[0001] The utility model relates to equipment for gene testing, in particular to a temperature control seat for preparing a gene sequencing library. Background Art

[0002] In order to control the reaction temperature of biochemical reagents in gene sequencing, the applicant has designed a refrigeration system for a gene sequencer.

[0003] A Chinese patent discloses a refrigeration system for a gene sequencer with an application number of CN201621166544.6. The refrigeration system of the gene sequencer includes: a reaction chamber shell, a refrigeration cover and a refrigeration device; the refrigeration cover is fixed inside the reaction chamber shell, and the refrigeration cover has an air inlet and an air outlet connected to the inside of the reaction chamber shell; the refrigeration device includes a refrigeration fin, a cold end fan and a cold end heat sink, the refrigeration fin is arranged outside the reaction chamber shell, the cold end heat sink is connected to the refrigeration surface of the refrigeration fin, and the cold end heat sink and the cold end fan are arranged inside the refrigeration cover; the cold end fan is located at the air inlet, and is used to inhale air in the reaction chamber shell through the air inlet; the cold end heat sink is arranged between the cold end fan and the air outlet, and the air outlet is used to discharge cold air into the reaction chamber shell.

[0004] Although the refrigeration system of the gene sequencer can achieve the effect of temperature control, the refrigeration system of the gene sequencer still has the following shortcomings: since the reaction chamber shell encloses a reaction chamber, a closed space, the cold end fan circulates the air in the reaction chamber to achieve the effect of temperature control of the reaction chamber, and the reaction chamber cannot be placed outside. The placement of the reaction chamber outside is the basis for the external automatic liquid suction and discharge equipment to act on the reaction chamber. Therefore, how to place the reaction chamber outside is an urgent problem to be solved. Utility Model Content

[0005] The utility model provides a temperature control seat for preparing a gene sequencing library, which solves the problem in the prior art that a reaction chamber cannot be installed outside a shell of a temperature-controlled reaction chamber.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] The utility model discloses a temperature control seat for preparing a gene sequencing library, comprising: a box seat, a temperature control execution device, a heat sink, a first fan, a second fan, a positioning seat and a clamping mechanism; the box seat is a tubular structure, a positioning seat is installed on the box seat, the positioning seat is used to place a detachable reagent storage reaction box, the temperature control execution device is installed below the positioning seat, the temperature control execution device adopts a refrigeration plate type, one side of the temperature control execution device is tightly attached to the positioning seat, and the other side of the temperature control execution device extends into an inner hole of the box seat; a clamping mechanism is installed on the box seat, and the clamping mechanism is used to clamp the reagent storage reaction box located on the positioning seat in a horizontal direction; a heat sink located below the temperature control execution device is installed in the box seat, a first fan is installed at one end of the box seat, and a second fan is installed at the other end of the box seat, and the first fan and the second fan draw outside air into the box seat and then discharge it.

[0008] Preferably, an ultrasonic transducer is installed in the box-type seat, and the output end of the ultrasonic transducer faces the reagent storage reaction box located on the positioning seat.

[0009] Preferably, the heat sink is provided with a receiving cavity, and the output end of the ultrasonic transducer passes through the receiving cavity.

[0010] Preferably, a guide rail is installed at the bottom of the box-type seat.

[0011] Preferably, the positioning seat includes: a first positioning block, a second positioning block and a third positioning block, the first positioning block and the second positioning block are both fan-shaped structures, the top surfaces of the first positioning block, the second positioning block and the third positioning block are all recessed to form a slot, the slot is for the bottom of the reagent storage reaction box to be inserted, there is a gap between the first positioning block and the second positioning block, there is a gap between the second positioning block and the third positioning block, and there is a gap between the first positioning block and the third positioning block.

[0012] Preferably, the temperature control execution device includes: a first group of Pelties, a second group of Pelties and a third group of Pelties, the first group of Pelties is located below the first positioning block, the second group of Pelties is located below the second positioning block, the third group of Pelties is located below the third positioning block, and the first group of Pelties, the second group of Pelties and the third group of Pelties are separated from each other.

[0013] Preferably, an arc-shaped notch is formed on one side of the third positioning block close to the clamping mechanism, and an inner wall of the arc-shaped notch cooperates with the clamping mechanism to clamp the bottom of the reagent storage reaction box.

[0014] Preferably, the clamping mechanism includes: a slide rail, a slider, a clamping arm, a clamping motor and a screw rod. The clamping motor is mounted on a box-type seat. The output shaft of the clamping motor is fixed to the screw rod. The screw rod is threadedly connected to the slider. The slider can be slidably mounted on the slide rail. The slide rail is mounted on the box-type seat. A clamping arm is fixed on the slider. The clamping arm is used to cooperate with the inner wall of the arc-shaped notch to clamp the bottom of the reagent storage reaction box.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] In the present application, the reagent storage reaction box provides a reaction chamber, so that the entire reaction chamber can be arranged outside the box seat (equivalent to the reaction chamber shell in the prior art), breaking the traditional thinking (the thinking that the entire reagent storage reaction box needs to be placed in the box seat), and realizing the externalization of the entire reagent storage reaction box. The refrigeration plate-type temperature control execution device on the box seat can control the temperature of the bottom of the reagent storage reaction box located on the positioning seat, so as to reach the temperature of the gene sequencing library preparation, and ensure that the reaction can proceed normally. In the present application, the temperature control execution device cools the reagent storage reaction box, and at the same time, heat is generated below the temperature control execution device, and the heat is gathered in the box seat. The first fan and the second fan are used to dissipate the heat of the box seat, so as to achieve rapid transfer of heat and avoid affecting the realization of the refrigeration function. The layout of the temperature control seat for preparing the entire gene sequencing library is completely different, which is convenient for replacing the reagent storage reaction box. And the entire heat dissipation circulation channel is arranged in the box seat, and the first fan and the second fan are arranged at the end of the box seat, which can reduce the volume of the temperature control seat for preparing the entire gene sequencing library. Compared with water cooling, it does not require the use of water, but only the use of the atmosphere. It can be used anytime and anywhere, making it more convenient to use.

[0017] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Schematic diagram of the structure when a reagent storage reaction box is installed in a temperature control seat for gene sequencing library preparation.

[0019] Figure 2 Schematic diagram of the structure of the temperature-controlled block for preparing gene sequencing libraries.

[0020] Figure numerals: box-type seat 1, temperature control actuator 2, first fan 3, second fan 4, positioning seat 5, first positioning block 51, second positioning block 52, third positioning block 53, arc-shaped notch 530, clamping mechanism 6, slide rail 61, slider 62, clamping arm 63, clamping motor 64, screw 65, reagent storage reaction box 7. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and functions of the present invention clearer and easier to understand, the present invention is further described below in conjunction with the accompanying drawings and specific implementation methods:

[0022] like Figure 1 as well as Figure 2As shown, the utility model discloses a temperature control seat for preparing a gene sequencing library, comprising: a box seat 1, a temperature control execution device 2, a heat sink, a first fan 3, a second fan 4 (not shown in the figure), a positioning seat 5 and a clamping mechanism 6; the box seat 1 is a tubular structure, a positioning seat 5 is installed on the box seat 1, the positioning seat 5 is used to place a detachable reagent storage reaction box 7, the temperature control execution device 2 is installed below the positioning seat 5, the temperature control execution device 2 adopts a refrigeration plate type, one side of the temperature control execution device 2 is tightly attached to the positioning seat 5, and the other side of the temperature control execution device 2 extends into the inner hole of the box seat 1; a clamping mechanism 6 is installed on the box seat 1, and the clamping mechanism 6 is used to clamp the reagent storage reaction box 7 located on the positioning seat 5 in a horizontal direction; a heat sink located below the temperature control execution device 2 is installed in the box seat 1, a first fan 3 is installed at one end of the box seat 1, and a second fan 4 is installed at the other end of the box seat 1, and the first fan 3 and the second fan 4 draw external air into the box seat 1 and then discharge it.

[0023] An ultrasonic transducer (not shown) is installed in the box-type seat 1, and the output end of the ultrasonic transducer faces the reagent storage reaction box 7 located on the positioning seat 5. The ultrasonic transducer converts electrical energy into mechanical energy, which can make the reagents in the reagent storage reaction box 7 evenly mixed to ensure the reaction effect.

[0024] The heat sink is provided with a receiving cavity for the output end of the ultrasonic transducer to pass through, so as to avoid the installation of the heat sink affecting the output end of the ultrasonic transducer from extending into a position close to the positioning seat 5, and ensure that the ultrasonic energy emitted by the ultrasonic transducer can mix the reagents evenly.

[0025] A guide rail (not shown) is installed at the bottom of the box-type seat 1. Since the entire gene sequencing library preparation temperature control seat needs to move to a position deviating from the external power drive, the external power drive is used to drive the reagent storage reaction box 7 to rotate.

[0026] The positioning seat 5 includes: a first positioning block 51, a second positioning block 52 and a third positioning block 53. The first positioning block 51 and the second positioning block 52 are both fan-shaped structures. The top surfaces of the first positioning block 51, the second positioning block 52 and the third positioning block 53 are all recessed to form a slot for inserting the bottom of the reagent storage reaction box 7. There is a gap between the first positioning block 51 and the second positioning block 52, there is a gap between the second positioning block 52 and the third positioning block 53, and there is a gap between the first positioning block 51 and the third positioning block 53. The three positions at the bottom of the reagent storage reaction box 7 are positioned at different positions.

[0027] The temperature control execution device 2 includes: a first group of Peltiers (not shown in the figure), a second group of Peltiers (not shown in the figure) and a third group of Peltiers (not shown in the figure), the first group of Peltiers is located below the first positioning block 51, the second group of Peltiers is located below the second positioning block 52, and the third group of Peltiers is located below the third positioning block 53, and the first group of Peltiers, the second group of Peltiers and the third group of Peltiers are separated from each other. Since the first positioning block 51, the second positioning block 52 and the third positioning block 53 are separately arranged and do not interfere with each other, the speed of heat transfer between the first positioning block 51, the second positioning block 52 and the third positioning block 53 is also reduced, so that the first positioning block 51, the second positioning block 52 and the third positioning block 53 can be controlled by the first group of Peltiers, the second group of Peltiers and the third group of Peltiers respectively, so as to ensure that the first positioning block 51, the second positioning block 52 and the third positioning block 53 can be at different temperatures, realize the temperature control of different positions respectively, and meet the needs of storing different reagents at different positions.

[0028] An arc-shaped notch 530 is formed on one side of the third positioning block 53 close to the pressing mechanism. The inner wall of the arc-shaped notch 530 cooperates with the clamping mechanism 6 to clamp the bottom of the reagent storage reaction box 7 .

[0029] The clamping mechanism 6 includes: a slide rail 61, a slider 62, a clamping arm 63, a clamping motor 64 and a screw 65. The clamping motor 64 is mounted on the box-type seat 1. The output shaft of the clamping motor 64 is fixed to the screw 65. The screw 65 is threadedly connected with the slider 62. The slider 62 is slidably mounted on the slide rail 61. The slide rail 61 is mounted on the box-type seat 1. The clamping arm 63 is fixed on the slider 62. The clamping arm 63 is used to cooperate with the inner wall of the arc-shaped notch 530 to clamp the bottom of the reagent storage reaction box 7. The clamping motor 64 drives the clamping arm 63 to move in the direction away from the arc-shaped notch 530 and close to the arc-shaped notch 530, thereby realizing the clamping work.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A temperature control station for preparing a gene sequencing library, characterized in that: include: A box-type seat (1), a temperature control actuator (2), a heat sink, a first fan (3), a second fan (4), a positioning seat (5), and a clamping mechanism (6); The box-type seat (1) is a tubular structure. A positioning seat (5) is installed on the box-type seat (1). The positioning seat (5) is used to place a detachable reagent storage reaction box (7). A temperature control execution device (2) is installed below the positioning seat (5). The temperature control execution device (2) adopts a refrigeration plate type. One side of the temperature control execution device (2) is tightly attached to the positioning seat (5), and the other side of the temperature control execution device (2) extends into the inner hole of the box-type seat (1). A clamping mechanism (6) is installed on the box-type seat (1), and the clamping mechanism (6) is used to clamp the reagent storage reaction box (7) located on the positioning seat (5) in the horizontal direction; A heat sink located below the temperature control actuator (2) is installed in the box-type seat (1), a first fan (3) is installed at one end of the box-type seat (1), and a second fan (4) is installed at the other end of the box-type seat (1). The first fan (3) and the second fan (4) draw outside air into the box-type seat (1) and then exhaust it.

2. The temperature control station for preparing a gene sequencing library according to claim 1, characterized in that: An ultrasonic transducer is installed in the box-type seat (1), and the output end of the ultrasonic transducer faces the reagent storage reaction box (7) located on the positioning seat (5).

3. The temperature control station for preparing a gene sequencing library according to claim 2, characterized in that: The heat sink is provided with a receiving cavity, through which the output end of the ultrasonic transducer passes.

4. The temperature control station for preparing a gene sequencing library according to any one of claims 1 to 3, characterized in that: A guide rail is installed at the bottom of the box-type seat (1).

5. The temperature control station for preparing a gene sequencing library according to claim 4, characterized in that: The positioning seat (5) comprises: a first positioning block (51), a second positioning block (52) and a third positioning block (53); the first positioning block (51) and the second positioning block (52) are both fan-shaped structures; the top surfaces of the first positioning block (51), the second positioning block (52) and the third positioning block (53) are all recessed to form a slot; the slot is for inserting the bottom of the reagent storage reaction box (7); there is a gap between the first positioning block (51) and the second positioning block (52), there is a gap between the second positioning block (52) and the third positioning block (53), and there is a gap between the first positioning block (51) and the third positioning block (53).

6. The temperature control station for preparing a gene sequencing library according to claim 5, characterized in that: The temperature control execution device (2) comprises: a first group of Peltiers, a second group of Peltiers and a third group of Peltiers, the first group of Peltiers being located below a first positioning block (51), the second group of Peltiers being located below a second positioning block (52), and the third group of Peltiers being located below a third positioning block (53), and the first group of Peltiers, the second group of Peltiers and the third group of Peltiers being separated from each other.

7. The temperature control station for preparing a gene sequencing library according to claim 6, characterized in that: The third positioning block (53) forms an arc-shaped notch (530) on one side close to the clamping mechanism, and the inner wall of the arc-shaped notch (530) cooperates with the clamping mechanism (6) to clamp the bottom of the reagent storage reaction box (7).

8. The temperature control station for preparing gene sequencing library according to claim 7, characterized in that: The clamping mechanism (6) comprises: A slide rail (61), a slider (62), a clamping arm (63), a clamping motor (64) and a screw (65), wherein the clamping motor (64) is mounted on the box-type seat (1), the output shaft of the clamping motor (64) is fixed to the screw (65), the screw (65) is threadedly connected to the slider (62), the slider (62) can be slidably mounted on the slide rail (61), the slide rail (61) is mounted on the box-type seat (1), and a clamping arm (63) is fixed on the slider (62), and the clamping arm (63) is used to cooperate with the inner wall of the arc-shaped notch (530) to clamp the bottom of the reagent storage reaction box (7).

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