Myocardial cell microenvironment mimicry culture dish

Abstract

The utility model relates to a myocardial cell microenvironment mimicry culture dish which comprises a dish body and a first separation membrane, the first separation membrane is arranged in the dish body and is used for separating a myocardial cell culture area and an interstitial cell culture area, and the first separation membrane can block cells but allows a culture solution to pass through. The myocardial cells and the interstitial cells are in the same culture environment but do not contact with each other. Compared with the prior art, the myocardial cell microenvironment mimicry culture dish provided by the utility model has the advantages that the myocardial cell culture area and the interstitial cell culture area are separated by utilizing the first separation membrane, and myocardial cells and interstitial cells can be cultured at the same time, so that the myocardial cells and the interstitial cells are cultured in the same culture solution but do not contact with each other; the cell state of two areas can be clearly observed, and the method is used for complex heart disease system research.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture dishes, in particular to a myocardial cell microenvironment mimicking culture dish. Background Art

[0002] In different heart diseases, the myocardial microenvironment is divided into immune microenvironment, microbial microenvironment, microcirculatory microenvironment and metabolic microenvironment. In the study of heart diseases, organ-intrinsic cells, interstitial cells (endothelial cells, fibroblasts, etc.), and immune cells (T cells, B cells, macrophages, etc.) jointly participate in the construction of the microenvironment.

[0003] In heart disease, the myocardial microenvironment is relatively complex, and most current studies cannot simultaneously study the relationship between the two types of cells due to limitations in experimental equipment. This may lead to one-sided results or conclusions and cannot well reflect the relationship between myocardial cells and interstitial cells in different heart diseases.

[0004] Therefore, in the study of myocardial microenvironment, it is urgent to design a culture device suitable for the interaction between cardiomyocytes and different interstitial cells, so as to provide a more comprehensive platform for the interaction between cells in the myocardial microenvironment. Utility Model Content

[0005] In view of this, it is necessary to provide a cardiomyocyte microenvironment mimicking culture dish to solve the technical problem that existing experimental equipment cannot simultaneously study the relationship between cardiomyocytes and interstitial cells.

[0006] The utility model provides a cardiomyocyte microenvironment mimic culture dish, which comprises a dish body and a first separation membrane. The first separation membrane is built in the dish body to separate a cardiomyocyte culture area and an interstitial cell culture area. The first separation membrane can block cells but allow culture fluid to pass through, so that the cardiomyocytes and interstitial cells are in the same culture environment but do not contact each other.

[0007] Furthermore, the first separation membrane is a porous membrane with a pore size less than 3 micrometers.

[0008] Furthermore, the first separation membrane is a polycarbonate porous membrane.

[0009] Furthermore, the first separation membrane is ring-shaped, and the interstitial cell culture area is arranged around the cardiomyocyte culture area.

[0010] Furthermore, it also includes a plurality of second separation membranes, which are arranged in the interstitial cell culture area to divide the interstitial cell culture area into a plurality of partitions.

[0011] Furthermore, the second partition membrane is detachably connected to the dish body to adjust the size and number of partitions.

[0012] Furthermore, it also includes a plurality of slot parts and a plurality of buckle parts. The slot parts are fixedly arranged in the interstitial cell culture area. A second separation membrane is arranged at the center of the buckle parts. The buckle parts are detachably connected to the slot parts to separate partitions in the interstitial cell culture area.

[0013] Furthermore, a plurality of slots are evenly distributed in the interstitial cell culture area so that each area has the same shape and size.

[0014] Furthermore, the dish body is a square, the first separation membrane is also a square, and the first separation membrane is arranged concentrically with the dish body.

[0015] Furthermore, the second separation membrane and the first separation membrane are the same kind of membrane.

[0016] Compared with the prior art, the cardiomyocyte microenvironment mimicking culture dish provided by the utility model utilizes a first separation membrane to separate a cardiomyocyte culture area and an interstitial cell culture area, and can culture cardiomyocytes and interstitial cells at the same time, so that cardiomyocytes and interstitial cells are cultured in the same culture medium but without contacting each other, and the cell states of the two regions can be clearly observed, which is used for the study of complex heart disease systems.

[0017] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the preferred embodiment of the utility model is described in detail as follows with the accompanying drawings. The specific implementation method of the utility model is given in detail by the following embodiments and their drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0019] Figure 1 This is a schematic structural diagram of an embodiment of a cardiomyocyte microenvironment mimicking culture dish provided by the utility model. DETAILED DESCRIPTION

[0020] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, wherein the accompanying drawings constitute a part of the present application and are used together with the embodiments of the present invention to illustrate the principles of the present invention, but are not used to limit the scope of the present invention.

[0021] See also Figure 1The utility model provides a myocardial cell microenvironment mimic culture dish, which provides a research platform for multi-cellular interactions in the myocardial microenvironment, making the cells involved in the research of the myocardial microenvironment more diverse. This new myocardial cell microenvironment mimic culture dish will improve the dimension of basic cell experimental research on the myocardial microenvironment, and the in vitro experiment will be closer to the in vivo research environment, which will provide strong technical support for microenvironment cell research.

[0022] The cardiomyocyte microenvironment mimic culture dish comprises a dish body 1 and a first separation membrane 2, wherein the first separation membrane 2 is built into the dish body 1 to separate a cardiomyocyte culture area 11 and an interstitial cell culture area 12. The first separation membrane 2 can block cells but allow the culture fluid to pass through, so that the cardiomyocytes and interstitial cells are in the same culture environment but do not contact each other. During the culture period, the cell states of the two regions can be clearly observed, which is used for the study of complex heart disease systems.

[0023] In order to achieve the effect that the nutrient solution can penetrate the first separation membrane 2, but the cells cannot penetrate the first sealing membrane 2, the first separation membrane 2 is a porous membrane with a pore size of less than 3 microns. In some embodiments, the first separation membrane 2 is a polycarbonate porous membrane. The polycarbonate membrane is a sterile membrane made under ultra-clean conditions using advanced dielectric technology and will not pollute the culture environment.

[0024] In some embodiments, the first separation membrane 2 is annular, and the interstitial cell culture area 12 is arranged around the cardiomyocyte 11 culture area.

[0025] In a preferred embodiment, there are also a plurality of second separators, which are arranged in the interstitial cell culture area 12 to separate the interstitial cell culture area 12 into a plurality of partitions. Different interstitial cells can be cultured in different partitions, thereby realizing the co-culture study of multiple interstitial cells and cardiomyocytes. The second separator and the first separator 2 can be made of the same membrane, i.e., a polycarbonate porous membrane.

[0026] The second partition membrane is preferably detachably connected to the dish body 1 so as to flexibly adjust the size and number of partitions according to different experimental requirements.

[0027] In some embodiments, the culture dish further has a plurality of slot portions 3 and a plurality of buckle portions 4. The slot portions 3 are fixedly disposed in the interstitial cell culture area 12. A second separation membrane is disposed at the center of the buckle portion 4. The buckle portion 4 is detachably connected to the slot portions 3 to separate partitions in the interstitial cell culture area.

[0028] In a preferred embodiment, a plurality of slots 3 are evenly distributed in the mesenchymal cell culture area 12 so that each area has the same shape and size.

[0029] In this embodiment, the dish body 1 is square, the first separation membrane 2 is also square, and the first separation membrane 2 is arranged concentrically with the dish body 1. The slot portion 3 is located at the center of each side, and equally divides the mesenchymal cell culture area 12. In other embodiments, the dish body 1 can also be circular, and the first separation membrane 2 is also circular.

[0030] Compared with the prior art, the cardiomyocyte microenvironment mimicking culture dish provided by the utility model utilizes a first separation membrane to separate a cardiomyocyte culture area and an interstitial cell culture area, and can culture cardiomyocytes and interstitial cells at the same time, so that cardiomyocytes and interstitial cells are cultured in the same culture medium but without contacting each other, and the cell states of the two regions can be clearly observed, which is used for the study of complex heart disease systems.

[0031] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed in the present invention should be included in the protection scope of the present invention.

Claims

1. A cardiomyocyte microenvironment mimic culture dish, characterized in that: It includes: The invention relates to a dish body and a first separation membrane, wherein the first separation membrane is built into the dish body to separate a cardiomyocyte culture area and an interstitial cell culture area, and the first separation membrane can block cells but allow culture fluid to pass through, so that the cardiomyocytes and interstitial cells are in the same culture environment but do not contact each other; the first separation membrane is annular, and the interstitial cell culture area is arranged around the cardiomyocyte culture area; and it also includes a plurality of second separation membranes, which are arranged in the interstitial cell culture area to divide the interstitial cell culture area into a plurality of partitions.

2. The cardiomyocyte microenvironment mimic culture dish according to claim 1, characterized in that: The first separation membrane is a porous membrane with a pore size less than 3 micrometers.

3. The cardiomyocyte microenvironment mimic culture dish according to claim 2, characterized in that: The first separation membrane is a polycarbonate porous membrane.

4. The cardiomyocyte microenvironment mimic culture dish according to claim 1, characterized in that: The second partition membrane is detachably connected to the dish body to adjust the size and number of the partitions.

5. The cardiomyocyte microenvironment mimic culture dish according to claim 4, characterized in that: It also includes a plurality of slot parts and a plurality of buckle parts, wherein the slot parts are fixedly arranged in the interstitial cell culture area, the second separation membrane is arranged at the center of the buckle part, and the buckle part is detachably connected to the slot parts to separate the partitions in the interstitial cell culture area.

6. The cardiomyocyte microenvironment mimic culture dish according to claim 5, characterized in that: The plurality of slots are evenly distributed in the interstitial cell culture area so that each of the partitions has the same shape and size.

7. The cardiomyocyte microenvironment mimic culture dish according to claim 1, characterized in that: The dish body is in a square shape, the first separation membrane is also in a square shape, and the first separation membrane is arranged concentrically with the dish body.

8. The cardiomyocyte microenvironment mimic culture dish according to claim 1, characterized in that: The second separation membrane is the same type of membrane as the first separation membrane.

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