Cell culture device for living cell imaging

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture device for living cell imaging, which comprises a culture dish, a bottom opening is arranged at the bottom of the culture dish, a temperature control probe is mounted and inserted at the right end of the culture dish, a top screw groove is arranged on the culture dish, and a temperature controller and a screw column are arranged on the side wall of the culture dish. A glass slide is arranged in the culture dish, a carrying groove, a lifting rod and a positioning insertion block are arranged on the glass slide, a locking groove is formed in the positioning insertion block, a culture medium is arranged in the carrying groove, a dish cover is arranged at the top end of the culture dish, and a heating plate is arranged in the side wall of the culture dish. The temperature in the culture dish is monitored in real time, the survival constant-temperature state temperature environment of living cells is guaranteed, the situation that the living cells die in the low-temperature state due to the fact that the temperature in the culture dish is reduced along with time is avoided, and then the imaging observation effect on living cell culture in the later period is guaranteed; the whole glass slide on the culture dish is convenient to disassemble, assemble and replace.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device for living cell imaging. Background Art

[0002] As the living cells are cultured and time passes, the experimenters will observe the dynamic imaging effects of the living cells through a microscope.

[0003] Generally, living cells are first placed in a culture dish for culture for a period of time, and then the culture dish containing the living cells is placed on the stage of a microscope. The living cells in the culture dish on the stage can be imaged and observed through the eyepiece and objective lens on the microscope. Therefore, before performing live cell imaging observation, a culture dish device for living cell culture is required.

[0004] At present, most of the existing culture dish devices for living cell culture do not have a constant temperature structure. The temperature in the culture dish will decrease over time, causing the living cells to die in a low temperature state, which will in turn affect the subsequent imaging observation effect of living cell culture.

[0005] In addition, a glass slide will be set on the culture dish, and the required culture medium will be set on the glass slide. The experimenter will place a type of living cells on the glass slide for culture in preparation for later imaging observation. After imaging and observing the type of living cells, a new type of living cells needs to be placed on the glass slide for culture. However, the culture medium on the glass slide has been used once and is dirty, and a new glass slide needs to be replaced. However, the existing culture dish devices for living cell culture are mostly inconvenient to disassemble and replace the glass slides on the culture dishes, which will cause dirt problems in the next living cell culture.

[0006] The above problems are widespread and urgently need to be improved. Utility Model Content

[0007] The purpose of the utility model is to solve the above-mentioned shortcomings in the prior art and to propose a cell culture device for living cell imaging.

[0008] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0009] A cell culture device for living cell imaging is designed, comprising a culture dish, a bottom opening is provided at the bottom of the culture dish, a temperature control probe is installed and inserted at the right end of the culture dish, a top screw groove is provided on the culture dish, a temperature controller and a screw column are provided on the side wall of the culture dish, a glass slide is provided in the culture dish, a loading groove, a lifting rod, and a positioning plug are provided on the glass slide, a locking groove is provided on the positioning plug, a culture medium is provided in the loading groove, a dish cover is provided at the top of the culture dish, and a heating plate is provided in the side wall of the culture dish.

[0010] Furthermore, the temperature controller is provided with a power cord, the temperature control probe, the temperature controller and the heating plate are electrically connected, and the heating plate is embedded in the side wall of the culture dish.

[0011] Furthermore, the positioning plug is plugged into the locking groove, the screw column is threadedly connected to the side wall of the culture dish, and the screw column is locked into the locking groove.

[0012] Furthermore, a turning handle is provided at the outer end of the screw column, and the turning handle is a diamond-shaped structure as a whole.

[0013] Furthermore, there are two lifting rods, which are symmetrically arranged at the top of the slide. The front cross-section of the lifting rod is an L-shaped structure, and the top of the lifting rod is lower than the dish cover.

[0014] Furthermore, a screw is inserted into the dish cover, a sealing gasket is arranged at the lower end of the dish cover, and the screw passes through the sealing gasket and is threadedly connected with the top screw groove.

[0015] Furthermore, the sealing gasket ring is made of polytetrafluoroethylene (PTFE), and the cross-section of the sealing gasket ring when viewed from above is a ring-shaped structure.

[0016] The utility model provides a cell culture device for living cell imaging, which has the following beneficial effects:

[0017] 1. The utility model realizes real-time monitoring of the temperature in the culture dish and ensures a constant temperature environment for the survival of living cells by arranging a temperature control and constant temperature monitoring heating structure on the culture dish, thereby avoiding the situation in which the temperature in the culture dish decreases over time and causes the living cells to die in a low temperature state, thereby ensuring the imaging observation effect of the living cell culture in the later stage.

[0018] 2. The utility model provides a card groove and a screw column on the culture dish, and provides a lifting rod and a positioning plug with a locking groove on the glass slide, so that the glass slide on the culture dish can be conveniently disassembled and replaced as a whole, thereby avoiding the problem of dirt in the next living cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is an exploded view of the overall structure of the utility model;

[0020] Figure 2 It is a three-dimensional schematic diagram of the overall structure installation state of the utility model;

[0021] Figure 3 For the utility model Figure 2 A front section view of the overall structure;

[0022] Figure 4 For the utility model Figure 1 A three-dimensional schematic diagram of the slide;

[0023] Figure 5 For the utility model Figure 1 A three-dimensional schematic diagram of the middle dish cover;

[0024] Figure 6 It is a block diagram of the utility model.

[0025] In the figure: 1 culture dish; 10 heating plate; 11 bottom opening; 12 top screw groove; 13 temperature control probe; 2 temperature controller; 21 power cord; 3 card groove; 4 screw column; 41 handle; 5 slide; 51 specimen tank; 52 culture medium; 53 lifting rod; 6 positioning plug; 61 locking groove; 7 dish cover; 71 screw; 72 sealing gasket. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0027] Reference Figure 1-6 A cell culture device for live cell imaging comprises a culture dish 1, a bottom opening 11 is provided at the bottom of the culture dish 1, a temperature control probe 13 is installed and inserted at the right end of the culture dish 1, a top screw groove 12 is provided on the culture dish 1, a temperature controller 2 and a screw column 4 are provided on the side wall of the culture dish 1, a glass slide 5 is provided in the culture dish 1, a loading groove 51, a lifting rod 53 and a positioning plug 6 are provided on the glass slide 5, a locking groove 61 is provided on the positioning plug 6, a culture medium 52 is provided in the loading groove 51, a dish cover 7 is provided at the top of the culture dish 1, and a heating plate 10 is provided in the side wall of the culture dish 1.

[0028] In detail, the temperature controller 2 is provided with a power cord 21, and the temperature control probe 13, the temperature controller 2, and the heating plate 10 are electrically connected. A PLC is preset in the temperature controller 2, and a temperature value can be preset on it. When the temperature control probe 13 detects that the temperature value in the culture dish 1 is lower than the preset temperature value on the PLC, the PLC will control the start-up of the heating plate 10 for rapid heating to ensure the normal living environment temperature of living cells.

[0029] The heating plate 10 is embedded in the side wall of the culture dish 1 . The heating plate 10 has a hollow cavity, wherein a dry-burning heating wire is preset in the cavity to achieve comprehensive and effective heating of the culture dish 1 .

[0030] Furthermore, the positioning block 6 is plugged into the locking groove 3, the screw column 4 is threadedly connected to the side wall of the culture dish 1, and the screw column 4 is engaged with the locking groove 61. When the screw column 4 withdraws from the locking groove 61, the screw column 4 is still in a threaded connection state with the side wall of the culture dish 1, so the loss of the screw column 4 can be avoided.

[0031] Furthermore, a gripping handle 41 is provided at the outer end of the screw column 4. The gripping handle 41 is a diamond-shaped structure as a whole, and can be directly grasped and rotated by hand to realize the forward and reverse rotation of the screw column 4, which is very convenient to use.

[0032] In more detail, there are two lifting rods 53 symmetrically disposed at the top of the glass slide 5 , and the front cross-section of the lifting rod 53 is an L-shaped structure, which is convenient for hooking and lifting with fingers.

[0033] The top end of the lifting rod 53 is lower than the dish cover 7 to avoid breaking the dish cover 7.

[0034] In general, a screw 71 is inserted into the dish cover 7, and a sealing gasket 72 is provided at the lower end of the dish cover 7. The screw 71 passes through the sealing gasket 72 and is threadedly connected to the top screw groove 12. There are four screws 71 to ensure that the dish cover 7 is stably installed on the culture dish 1.

[0035] Finally, the sealing gasket ring 72 is made of polytetrafluoroethylene (PTFE), and the cross-section of the sealing gasket ring 72 when viewed from above is a ring-shaped structure. The polytetrafluoroethylene (PTFE) material has excellent high elastic sealing properties, which ensures the sealing between the dish cover 7 and the culture dish 1, and is heat-resistant and chemical-resistant.

[0036] Working method: first put the living cells into the specimen tank 51 with the culture medium 52, then put the glass slide 5 into the culture dish 1 as a whole, ensure that the positioning plug 6 is inserted into the card groove 3, and then lock the screw column 4 clockwise to achieve the screw column 4 being inserted into the locking groove 61 for positioning, and then cover the culture dish 1 with the dish cover 7 and lock the screw 71 clockwise.

[0037] The power cord 21 is connected to an external power supply and turned on, and the temperature controller 2 and the temperature control probe 13 are powered on for use. The temperature control probe 13 will monitor the ambient temperature in the culture dish 1 in real time, and will transmit the monitored temperature data to the PLC in the temperature controller 2 in real time. After a period of cultivation, if the ambient temperature in the culture dish 1 is lower than the preset temperature value on the PLC in the temperature controller 2, the PLC will control the start of the heating plate 10, and the heating wire in the heating plate 10 will be powered on to heat and heat the culture dish 1 to ensure that the temperature value is suitable for the living environment temperature of living cells, thereby realizing real-time monitoring of the temperature in the culture dish 1 and ensuring a constant temperature environment for the survival of living cells, avoiding the situation in which the temperature in the culture dish 1 decreases over time and causes the living cells to die in a low temperature state, thereby ensuring the imaging observation effect of the living cell culture in the later stage.

[0038] In addition, if after the current type of living cell imaging observation is completed, a new type of living cells needs to be placed on the glass slide 5 for culture, but the culture medium 52 on the glass slide 5 has been used once and is dirty, and a new glass slide 5 needs to be replaced. First, turn the screw 71 counterclockwise to remove it, then open and remove the dish cover 7, and then turn the handles 41 on both sides counterclockwise to drive the screw column 4 to rotate counterclockwise, and drive the screw column 4 to move outward and exit the locking groove 61 through the threaded transmission between the side wall of the culture dish 1, and then hook the lifting rod 53 with both hands and pull it upward, so that the glass slide 5 can be conveniently taken out of the culture dish 1 as a whole. Later, the glass slide 5 with the new culture medium 52 can be conveniently installed in the culture dish 1 as a whole, so that the glass slide 5 on the culture dish 1 can be conveniently disassembled and replaced as a whole, thereby avoiding the problem of dirt in the next living cell culture.

[0039] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture device for live cell imaging, comprising a culture dish (1), characterized in that: The bottom of the culture dish (1) is provided with a bottom opening (11), the right end of the culture dish (1) is installed with a temperature control probe (13), the culture dish (1) is provided with a top screw groove (12), the side wall of the culture dish (1) is provided with a temperature controller (2) and a screw column (4), a glass slide (5) is provided in the culture dish (1), the glass slide (5) is provided with a loading groove (51), a lifting rod (53), and a positioning plug (6), the positioning plug (6) is provided with a locking groove (61), a culture medium (52) is provided in the loading groove (51), a dish cover (7) is provided at the top end of the culture dish (1), and a heating plate (10) is provided in the side wall of the culture dish (1).

2. A cell culture device for living cell imaging according to claim 1, characterized in that: The temperature controller (2) is provided with a power line (21), the temperature control probe (13), the temperature controller (2) and the heating plate (10) are electrically connected, and the heating plate (10) is embedded in the side wall of the culture dish (1).

3. A cell culture device for living cell imaging according to claim 1, characterized in that: The positioning plug block (6) is plugged into the locking groove (3), the screw column (4) is threadedly connected to the side wall of the culture dish (1), and the screw column (4) is locked into the locking groove (61).

4. The cell culture device for living cell imaging according to claim 1, characterized in that: The outer end of the screw column (4) is provided with a handle (41), and the handle (41) is a diamond-shaped structure as a whole.

5. The cell culture device for living cell imaging according to claim 1, characterized in that: There are two lifting rods (53) which are symmetrically arranged at the top of the glass slide (5). The front cross-section of the lifting rod (53) is an L-shaped structure, and the top of the lifting rod (53) is lower than the dish cover (7).

6. The cell culture device for living cell imaging according to claim 1, characterized in that: A screw (71) is inserted into the dish cover (7), a sealing ring (72) is provided at the lower end of the dish cover (7), and the screw (71) passes through the sealing ring (72) and is threadedly connected to the top screw groove (12).

7. A cell culture device for living cell imaging according to claim 6, characterized in that: The sealing gasket ring (72) is made of polytetrafluoroethylene (PTFE), and the cross-section of the sealing gasket ring (72) when viewed from above is a ring-shaped structure.

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