Three-color immunochromatography detection card

Abstract

The utility model discloses a three-color immunochromatography detection card, which comprises a card support base, a detection card and a detection card, a reaction buffer area is arranged in the middle of the bottom plate, a sample pad, a first colored latex marking pad, a second colored latex marking pad, a third colored latex marking pad and a connection pad which are sequentially overlapped are arranged on the bottom plate on one side of the reaction buffer area from left to right, and a chromatography film and a water absorption pad which are sequentially overlapped are arranged on the bottom plate on the other side of the reaction buffer area from left to right; three detection lines and quality control lines are arranged on the chromatography membrane; the movable part is arranged on the reaction buffer area, and the bottom surface of the connecting pad is lapped at the top end of the movable part; a pressing part is arranged on the upper cover, and a protruding part is arranged on the bottom face of the pressing part. The utility model provides a three-color immunochromatography detection card which can prolong the reaction time of a marker and a detected object on a marker pad under the condition of not increasing operation, so that the detection sensitivity is improved, meanwhile, multi-item detection can be realized, and the detection efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of detection cards, in particular to a three-color immunochromatographic detection card. Background Art

[0002] The test card is commonly used in drug, virus, blood and other sampling situations due to its portability and rapid testing characteristics. It mainly includes an upper cover, a bottom and a test paper placed in the inner cavity of the upper cover and the bottom. The upper cover is usually provided with a sample adding window and an observation window. The operator drops a certain amount of sample liquid into the sample adding window and it falls on the sample pad of the test paper. The sample liquid gradually stratifies along the direction of the test paper strip. The observation window allows the operator to observe the test results of the test paper.

[0003] In the prior art, general test cards cannot meet the requirements of multiple project detection, that is, one test card is required to detect one project, and multiple test cards are required to detect multiple projects. In order to realize multi-project detection, some test cards are provided with multiple test strips on the same base to realize that one test card can detect multiple projects, but this kind of test card requires multiple operations to add samples to achieve it, and the general test paper adopts a sample pad, a gold label pad, a chromatography membrane, and a blotting paper connected in sequence. In this way, the sample liquid is dripped onto the sample pad, and the liquid flows rapidly along the direction of the test strip for chromatography. After chromatography on the gold label pad, the gold label substance on the gold label pad will be chromatographed onto the NC membrane together with the sample liquid. In this case, the gold label substance does not have enough time to fully combine with the target substance in the sample liquid, and will be chromatographed to the T line binding position on the NC membrane, reducing the detection sensitivity. Utility Model Content

[0004] An object of the present invention is to solve at least the above problems and / or disadvantages and to provide at least the advantages to be described below.

[0005] In order to solve the deficiencies of the prior art, the utility model provides a three-color immunochromatographic detection card, comprising:

[0006] A card holder bottom, on which a bottom plate is fixed; a reaction buffer is arranged in the middle of the bottom plate, a sample pad, a first color latex marker pad, a second color latex marker pad, a third color latex marker pad and a connection pad are arranged on the bottom plate on one side of the reaction buffer from left to right in sequence, and a chromatography membrane and a water absorption pad are arranged on the bottom plate on the other side in sequence from left to right; three detection lines and a quality control line are arranged on the chromatography membrane;

[0007] A movable part, which is arranged on the reaction buffer area and the bottom surface of the connection pad is placed on the top of the movable part;

[0008] The upper cover is detachably connected to the card holder bottom and is provided with a sample addition hole and a detection window; the upper cover is provided with a pressing portion, and the bottom surface of the pressing portion is provided with a protrusion that can make the connection pad fall to the right and contact the chromatographic membrane.

[0009] Furthermore, the three test lines are respectively coated with complete antigens of different test items, and the quality control line is coated with goat anti-mouse IgG.

[0010] Furthermore, the lower surface of the protrusion is an inclined portion that matches the inclined surface of the engagement pad.

[0011] Furthermore, a protrusion is provided on the right side of the inclined portion.

[0012] Furthermore, the movable part is arranged to be inclined to the right.

[0013] Furthermore, the bottom plate is made of PVC material.

[0014] Furthermore, the sample pad is made of glass cellulose membrane or polyester membrane.

[0015] Furthermore, the marking pad adopts a glass cellulose film or a polyester film.

[0016] Furthermore, the chromatography membrane is a backed nitrocellulose membrane.

[0017] Furthermore, the absorbent pad is made of strong absorbent paper.

[0018] The utility model at least includes the following beneficial effects: the bottom of the card holder is used for loading the bottom plate, the upper cover and the bottom of the card holder form a cavity to accommodate the sample pad, the first color latex marker pad, the second color latex marker pad, the third color latex marker pad, the connecting pad, the chromatographic membrane, the water absorbent pad and the movable part, the sample addition hole of the upper cover is used to facilitate the dripping of the sample liquid onto the sample pad, and the detection window is used to observe the detection result on the chromatographic membrane; the arrangement of the first color latex marker pad, the second color latex marker pad, the third color latex marker pad and the three detection lines on the chromatographic membrane enables the utility model to realize multi-item detection without increasing the operation, thereby improving the detection efficiency; and the sensitivity is increased by 2-3 times by the corresponding colloidal gold method through the color microsphere color development, and at the same time, the first color latex marker pad, the second color latex marker pad and the third color latex marker pad can be divided into different color zones Different detection items are distinguished to facilitate result judgment and production without errors, thereby improving detection accuracy; in addition, the reaction buffer zone and the connecting pad are cooperated to separate the chromatographic membrane, so that the sample liquid flows through the first color latex marker pad, the second color latex marker pad, the third color latex marker pad and the connecting pad in sequence, and the connecting pad can prolong the reaction binding time, and the connecting pad is supported by the movable part to be separated from the chromatographic membrane, and after a period of reaction, the pressing part of the upper cover can be pressed hard so that the raised part on the bottom of the pressing part presses the connecting pad downward, and the movable part falls to the right under the pressure, so that the connecting pad falls to the right and contacts with the chromatographic membrane, and then continues the chromatography, thereby increasing the reaction time of the sample and the antibody-labeled latex microspheres, allowing the sample liquid to react with the antibody-labeled latex microspheres, increasing the reaction sensitivity to fully react, and increasing the reaction sensitivity.

[0019] During use, the sample liquid drips onto the sample pad through the sample loading hole, and then flows to the right through the first color latex marker pad, the second color latex marker pad, and the third color latex marker pad in turn, and then flows into the connecting pad. After a period of reaction, the operator presses the pressing part hard to make the protrusion press on the connecting pad. Under the action of pressure, the movable part falls to the right. At this time, the connecting pad falls to the right and contacts the chromatographic membrane. The operator removes the pressure on the pressing part, and the protrusion separates from the connecting pad. The liquid that has fully reacted with the antibody-labeled latex microspheres enters the chromatographic membrane for chromatography, and the test results are viewed through the detection window. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of a three-color immunochromatographic detection card of the utility model.

[0021] Figure numerals: 100-card tray bottom, 200-bottom plate, 210-sample pad, 220-first color latex marking pad, 230-second color latex marking pad, 240-third color latex marking pad, 250-connecting pad, 260-chromatographic membrane, 261-detection line, 262-quality control line, 270-water absorbent pad, 300-buffer zone, 400-movable part, 500-upper cover, 510-sample loading hole, 520-detection window, 530-pressing portion, 540-protrusion, 541-inclined portion, 542-protrusion. DETAILED DESCRIPTION

[0022] The present invention will be further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0023] It should be understood that terms such as “having”, “including” and “comprising” used herein do not specify the existence or addition of one or more other elements or combinations thereof.

[0024] See also Figure 1 , a three-color immunochromatographic test card, comprising:

[0025] The tray bottom 100 is fixed with a bottom plate 200 made of PVC; a reaction buffer 300 is arranged in the middle of the bottom plate 200, and a sample pad 210, a first color latex marking pad 220, a second color latex marking pad 230, a third color latex marking pad 240 and a connecting pad 250 are arranged on the bottom plate 200 on one side of the reaction buffer 300 from left to right, and a chromatography membrane 260 and a water-absorbing pad 270 are arranged on the bottom plate 200 on the other side from left to right, the sample pad 210 is made of glass cellulose membrane or polyester membrane, and the chromatography membrane 260 and a water-absorbing pad 270 are arranged on the bottom plate 200 on the other side. 260 uses a backing nitrocellulose membrane, and the marking pad uses a glass cellulose membrane or a polyester membrane; three detection lines 261 and a quality control line 262 are set on the chromatographic membrane 260. Specifically, the three detection lines 261 are respectively coated with complete antigens of different detection items, and the quality control line 262 is coated with goat anti-mouse IgG. It can be understood that the three detection lines 261 are respectively detection line T1 coated with complete antigens of detection item 1, detection line T2 coated with complete antigens of detection item 2, and detection line T3 coated with complete antigens of detection item 3. Detection items 1-3 are selected according to actual needs;

[0026] The movable part 400 is arranged on the reaction buffer 300 and the bottom surface of the connection pad 250 is placed on the top of the movable part 400. It is worth noting that the movable part 400 can be formed by injection molding of a plastic part, as long as the movable part 400 is pressed and falls to the right;

[0027] The upper cover 500 is detachably connected to the card holder bottom 100 and is provided with a sample addition hole 510 and a detection window 520. The detachable connection between the upper cover 500 and the card holder bottom 100 belongs to the prior art. The sample addition hole 510 of the upper cover 500 is used to facilitate dripping of sample liquid onto the sample pad 210, and the detection window 520 is used to observe the detection result on the chromatographic membrane 260. The upper cover 500 is provided with a pressing portion 530, and the bottom surface of the pressing portion 530 is provided with a The connection pad 250 falls to the right and contacts the raised portion 540 of the chromatography membrane 260. It is worth noting that the pressing portion 530 can be a part of the upper cover 500, and the pressing portion 530 can be moved downward by pressing hard. Of course, it can also be a push-type button structure or other elastic member. The push-type button belongs to the prior art product, as long as the pressing portion 530 can move toward the card holder bottom 100 under the external force of the operator and can return to its original position after the operator removes the external force.

[0028] Specifically, the card tray bottom 100 is used to load the bottom plate 200, and the upper cover 500 and the card tray bottom 100 form a cavity to accommodate the sample pad 210, the first color latex marking pad 220, the second color latex marking pad 230, the third color latex marking pad 240, the connecting pad 250, the chromatography membrane 260, the absorbent pad 270 and the movable part 400. The arrangement of the first color latex marking pad 220, the second color latex marking pad 230, the third color latex marking pad 240 and the three detection lines on the chromatography membrane enables the utility model to realize multi-item detection without increasing the operation, thereby improving the detection efficiency; and the sensitivity of the corresponding colloidal gold method is increased by 2-3 times through the color microsphere color development, and the first color The latex marking pad 220, the second color latex marking pad 230, and the third color latex marking pad 240 can distinguish different detection items through different colors, which is convenient for result judgment and production without mistakes, and improves detection accuracy. It can be understood that the latex on the first color latex marking pad 220 is red latex, the latex on the second color latex marking pad 230 is blue latex, and the latex on the third color latex marking pad 230 is black latex. Of course, it can also be that the latex on the first color latex marking pad 220 is blue latex, the latex on the second color latex marking pad 230 is red latex, and the latex on the third color latex marking pad 230 is black latex. The selection and order of colors are not specifically limited;

[0029] In addition, the reaction buffer 300 and the connecting pad 250 cooperate to separate the chromatographic membrane, so that the sample liquid flows through the first color latex marking pad 220, the second color latex marking pad 230, the third color latex marking pad 240 and the connecting pad 250 in sequence. The connecting pad 250 can prolong the reaction binding time, and the connecting pad 250 is supported by the movable part 400 and is separated from the chromatographic membrane 260. After a period of reaction, the pressing part 530 of the upper cover 100 can be pressed hard so that the raised part 540 on the bottom surface of the pressing part 530 presses the connecting pad 250 downward. Under the action of pressure, the movable part 400 falls to the right, and the connecting pad 250 loses the support of the movable part 400, so that the connecting pad 250 falls to the right and contacts with the chromatographic membrane 260, and then continues the chromatography, thereby increasing the reaction time between the sample and the antibody-labeled latex microspheres, allowing the sample liquid to react with the antibody-labeled latex microspheres, increasing the reaction sensitivity, and fully reacting, thereby increasing the reaction sensitivity.

[0030] In the above embodiment, the lower surface of the protrusion 540 is an inclined portion 541 that cooperates with the inclined surface of the connection pad 250. With this technical solution, the setting of the inclined portion 541 ensures that the movable part 400 falls to the right, thereby ensuring that the connection pad 250 contacts the chromatographic membrane 260 smoothly.

[0031] Furthermore, a protrusion 542 is provided on the right side of the inclined portion 541 .

[0032] In order to further enable the movable member 400 to fall rightward smoothly under pressure, the movable member 400 is tilted to the right.

[0033] When the utility model is used, the sample liquid drips onto the sample pad 210 through the sample addition hole, and then flows to the right through the first color latex marking pad 220, the second color latex marking pad 230, and the third color latex marking pad 240 in sequence, and then flows into the connecting pad 250. After a period of reaction, the operator presses the pressing part 530 hard, so that the protrusion 540 presses on the connecting pad 250. Under the action of pressure, the movable part 400 falls to the right. At this time, the connecting pad 250 falls to the right and contacts the chromatographic membrane 260. The operator removes the pressure on the pressing part 530, and the protrusion 540 separates from the connecting pad 250. The liquid that has fully reacted with the antibody-labeled latex microspheres enters the chromatographic membrane 260 for chromatography, and the detection result is viewed through the detection window 520.

[0034] Although the implementation scheme of the utility model has been disclosed as above, it is not limited to the applications listed in the specification and implementation scheme. It can be fully applied to various fields suitable for the utility model. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the utility model is not limited to the specific details and the illustrations shown and described here. Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention.

Claims

1. A three-color immunochromatographic test card, characterized in that: include: A card holder bottom (100) is fixed with a bottom plate (200); a reaction buffer zone (300) is arranged in the middle of the bottom plate (200); a sample pad (210), a first color latex marking pad (220), a second color latex marking pad (230), a third color latex marking pad (240) and a connection pad (250) are arranged on the bottom plate (200) on one side of the reaction buffer zone (300) from left to right, and a chromatographic membrane (260) and a water absorption pad (270) are arranged on the bottom plate (200) on the other side from left to right; three detection lines (261) and a quality control line (262) are arranged on the chromatographic membrane (260); A movable part (400) is arranged on the reaction buffer zone (300) and the bottom surface of the connection pad (250) rests on the top of the movable part (400); The upper cover (500) is detachably connected to the card holder bottom (100) and is provided with a sample loading hole (510) and a detection window (520); the upper cover (500) is provided with a pressing portion (530), and the bottom surface of the pressing portion (530) is provided with a protrusion (540) that can make the connection pad (250) fall to the right and contact the chromatographic membrane (260).

2. A three-color immunochromatographic test card according to claim 1, characterized in that: The three detection lines (261) are respectively coated with complete antigens of different detection items, and the quality control line (262) is coated with goat anti-mouse IgG.

3. A three-color immunochromatographic test card according to claim 1, characterized in that: The lower surface of the protruding portion (540) is an inclined portion (541) that matches the inclined surface of the connection pad (250).

4. A three-color immunochromatographic test card according to claim 3, characterized in that: A protrusion (542) is provided on the right side of the inclined portion (541).

5. A three-color immunochromatographic test card according to claim 4, characterized in that: The movable member (400) is arranged to be tilted to the right.

6. A three-color immunochromatographic test card according to claim 1, characterized in that: The bottom plate (200) is made of PVC material.

7. A three-color immunochromatographic test card according to claim 1, characterized in that: The sample pad (210) is made of glass cellulose film or polyester film.

8. A three-color immunochromatographic test card according to claim 1, characterized in that: The marking pad is made of glass cellulose film or polyester film.

9. A three-color immunochromatographic test card according to claim 1, characterized in that: The chromatographic membrane (260) is a backed nitrocellulose membrane.

10. A three-color immunochromatographic test card according to claim 1, characterized in that: The absorbent pad (270) is made of strong absorbent paper.

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