Counting device under low-power optical microscope
By designing the boss, liquid adding area and counting area on the slide, combined with the counting scale, the problem of inaccurate counting under low-power optical microscope is solved, and a high accuracy and low-cost counting device is achieved, suitable for 40 times Optical microscope under the microscope.
Patent Information
- Application Number
- CN202323491823.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-21
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2033-12-21
AI Technical Summary
Under low-power optical microscopes, it is difficult for the prior art to accurately count larger objects in the solution, which are prone to leakage or repeated counting, and the sample dilution ratio requirements are high, resulting in detection errors.
A glass slide is designed, including a boss, liquid filling area and a counting area. The counting area is equipped with long-shaped counting holes and counting scales distributed in parallel and equidistant distances. It is suitable for 40x microscopes, providing positioning references and reducing Move direction to improve counting accuracy.
It improves the accuracy and efficiency of counting, reduces the requirements for sample dilution, has a wide volume range, and the device is made of plexiglass, which has a low cost of use, and is suitable for reuse.
Smart Images
Figure CN222274567U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of biological instruments, and in particular relates to a counting device under a low-power optical microscope. Background Art
[0002] At present, for larger objects in the solution, when counting under a 40x microscope, a certain amount of solution is usually sucked by a pipette, drawn on a glass slide in the form of a waterline, and counted by moving the field of view up and down and left and right. However, the edge of the waterline is irregular and the area is large. Under a 40x microscope, the field of view must be carefully moved up and down and left and right. In the absence of a reference, it is very likely to miss or repeat the count; or a certain amount of solution is added to a 96-well plate for counting, but the 96-well plate has a large aperture, and the sample solution cannot be fully displayed under the same microscope field of view. It is also necessary to move the field of view up and down and left and right for counting, resulting in double counting or omission errors. At the same time, both methods have high requirements for the dilution multiple of the sample. The sample solution needs to be diluted to an appropriate multiple in order to count as accurately as possible. If the dilution multiple is low, the number of samples in the solution is large, which is easy to cause double counting and omission. If the dilution multiple is high, the number of samples in the solution is small, which is easy to cause counting errors. Therefore, missed detection or repeated detection often occurs in the detection, and the objects in it cannot be accurately counted, especially when the detection object is a living microorganism.
[0003] Therefore, designing a counting device that is convenient for observation, accurate in counting, and suitable for use under a low-power optical microscope is of great significance to the art. Utility Model Content
[0004] In view of the deficiencies in the prior art, the utility model provides a counting device under a low-power optical microscope, which effectively solves the problems of inaccurate counting such as double counting and missed counting when counting sample liquid under an optical microscope.
[0005] The technical solution provided by the utility model is as follows:
[0006] A counting device under a low-power optical microscope comprises a glass slide, on which a boss, a liquid adding area and a counting area are arranged, wherein the liquid adding area is located between two bosses, the liquid adding area is connected with the counting area, and the counting area is provided with counting scale lines.
[0007] Furthermore, the glass slide is 75 mm long, 25 mm wide, and 3 mm high, and is used for counting under a 40x optical microscope.
[0008] Furthermore, the liquid adding area is a groove, and the liquid adding area is used to add the sample liquid to the liquid adding area.
[0009] Furthermore, the bottom surface of the liquid adding area is 0.1 mm lower than the upper surface of the boss.
[0010] Furthermore, the counting area is a long strip of counting holes that are distributed in parallel and at equal distances, and the counting area is used to observe and detect the sample liquid.
[0011] Furthermore, the upper surface of the counting area and the bottom surface of the liquid adding area are on the same plane.
[0012] Furthermore, the number of the counting areas and the number of the liquid adding areas are both 3.
[0013] Furthermore, the counting area is 50 mm long, 3.5 mm wide and 1.5 mm high.
[0014] Furthermore, the distance between adjacent short scale lines of the counting scale is 2 mm, and the distance between adjacent long scale lines of the counting scale is 10 mm.
[0015] Furthermore, the capacity of the counting area is 50 to 200 μL.
[0016] Compared with the prior art, the utility model has the following beneficial effects:
[0017] (1) The utility model provides a counting device for low-power optical microscope, which has high counting accuracy. Under a 40x optical microscope, the width of the long strip counting hole in the counting area designed by the utility model is just the width of a field of view, and the field of view is clear and not easy to be confused. When counting, it only needs to move in one direction (left or right). At the same time, counting scale lines are provided in the counting area, which can provide positioning references when moving left and right, greatly improving the counting accuracy and efficiency. At the same time, three counting holes are provided, which is more statistically significant, making the calculation results more accurate.
[0018] (2) The quantitative counting device provided by the utility model has low requirements on the degree of sample dilution. Each counting hole can be added with 50 to 200 μL of solution, and the detection solution volume range is wide.
[0019] (3) The quantitative counting device provided by the utility model has low use cost. The quantitative counting device is made of organic glass and can be sterilized under high pressure and reused. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the overall structure of a counting device under a low-power optical microscope of the utility model;
[0021] Figure 2 This is a front view of a counting device under a low-power optical microscope of the utility model;
[0022] Figure 3 This is a right view of a counting device under a low-power optical microscope of the utility model;
[0023] In the figure: 1 slide glass, 11 boss, 12 counting area, 13 liquid adding area, 14 counting scale line. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] The utility model provides a counting device under a low-power optical microscope. The technical solution of the utility model is further described below in conjunction with the accompanying drawings:
[0026] See also Figure 1 , Figure 2 and Figure 3 It can be seen that the utility model provides a counting device under a low-power optical microscope, including a glass slide 1, on which a boss 11, a liquid adding area 13 and a counting area 12 are provided. The liquid adding area 13 is located between two bosses 11, the liquid adding area 13 is connected to the counting area 12, and the counting area 12 is provided with a counting scale line 14.
[0027] The glass slide 1 is 75 mm long, 25 mm wide, and 3 mm high, and is used for counting under an optical microscope at 40 times magnification.
[0028] See also Figure 1 and Figure 3 It can be seen that the liquid adding area 13 is a groove, which is used to add the sample liquid to the liquid adding area; the bottom surface of the liquid adding area 13 is 0.1 mm lower than the upper surface of the boss 11 to prevent the sample liquid from flowing out when adding the sample liquid to the liquid adding area.
[0029] See also Figure 1 , Figure 2 and Figure 3 It can be seen that the counting area 12 is a long strip counting hole distributed in parallel and at equal distances, and its function is to observe and detect the sample liquid.
[0030] Among them, the upper surface of the counting area 12 and the bottom surface of the liquid adding area 13 are on the same plane, which is convenient for the sample liquid to flow from the liquid adding area to the counting area; the number of the counting area 12 and the liquid adding area 13 are both 3; the counting area 12 is 50mm long, 3.5mm wide, and 1.5mm high; the distance between adjacent short scale lines of the counting scale 14 is 2mm, and the distance between adjacent long scale lines of the counting scale 14 is 10mm; the capacity of the counting area 12 is 50-200μL.
[0031] When using, when counting objects in the sample liquid, after the sample liquid is appropriately diluted, 50-200 μL of the sample liquid is evenly dripped into the groove of the liquid adding area 13 of the slide glass 1, and flows to a counting hole in the counting area 12, and then the slide glass is moved to an optical microscope under a 40x lens, and the counting can be performed by moving the field of view from left to right from the counting hole without moving up and down; to ensure the counting accuracy, equal volumes of sample liquid can be added to different counting holes to repeat the counting and calculate the average value. The counting device provided by the utility model has a simple structure, convenient observation, and accurate counting.
[0032] The above is a detailed introduction to a counting device under a low-power optical microscope provided by the utility model. The above embodiments are only used to illustrate the technical solution of the utility model but not to limit it. Those skilled in the art should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, and should be included in the scope of the claims of the utility model.
Claims
1. A counting device under a low-power optical microscope, comprising a slide glass (1), characterized in that: The glass slide (1) is provided with a boss (11), a liquid adding area (13) and a counting area (12); the liquid adding area (13) is located between the two bosses (11), the liquid adding area (13) is connected to the counting area (12), and the counting area (12) is provided with a counting scale line (14); the glass slide (1) is 75 mm long, 25 mm wide and 3 mm high, and the glass slide (1) is used for counting under a 40x optical microscope; the liquid adding area (13) is a groove, and the liquid adding area (13) is used to add sample liquid to the liquid adding area, and the bottom surface of the liquid adding area (13) is 0.1 mm lower than the upper surface of the boss (11); the upper surface of the counting area (12) and the bottom surface of the liquid adding area (13) are on the same plane, and the counting area (12) is 50 mm long, 3.5 mm wide and 1.5 mm high.
2. The counting device under a low-power optical microscope according to claim 1, characterized in that: The counting area (12) is a long strip of counting holes that are distributed in parallel and at equal distances. The counting area (12) is used to observe and detect the sample liquid.
3. The counting device under a low-power optical microscope according to claim 2, characterized in that: The number of the counting areas (12) and the number of the liquid adding areas (13) are both three.
4. The counting device under a low-power optical microscope according to claim 3, characterized in that: The distance between adjacent short scale lines of the counting scale lines (14) is 2 mm, and the distance between adjacent long scale lines of the counting scale lines (14) is 10 mm.
5. The counting device under a low-power optical microscope according to claim 4, characterized in that: The capacity of the counting area (12) is 50 to 200 μL.