Tandem type cell culture bag
Through the design of the tandem cell culture bag, the joint and volume enhancing bag are used to form a tandem pathway, which solves the problem of increasing capacity during cell proliferation, reduces the risk of bacterial contamination during transplantation, and improves the cell culture bag through fire-proof and anti-corrosion materials The durability of the cell culture bag is achieved by non-transplant increase in cell culture bag capacity.
Patent Information
- Application Number
- CN202420564733.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-22
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2034-03-22
AI Technical Summary
The existing cell culture bags have a larger capacity when cells proliferate and require frequent transplantation, which can easily lead to bacterial contamination and cannot increase the capacity of cell culture bags by non-transplantation, affecting the use of tandem cell culture.
A tandem cell culture bag is designed, including a fixedly connected inlet and outlet pipe, sampling pipe and tandem volume enhancement mechanism, through a first concave cap joint, a first concave cap joint, a second concave cap joint and a cell capacity enhancement bag. The arrangement of the device forms a tandem pathway to avoid secondary cell transplantation and wrap fire-resistant and anti-corrosion breathable materials on the outer surface to improve durability.
The tandem increase of cell proliferation capacity during cell proliferation is achieved, reducing the risk of bacterial contamination during transplantation, and improving the durability and safety of cell culture bags through fire-resistant, anti-corrosion and breathable materials.
Smart Images
Figure CN222274580U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field related to cell culture bags, in particular to a series cell culture bag. Background Art
[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Cell culture is an indispensable process for both the entire bioengineering technology and one of the biological cloning technologies. Cell culture itself is the large-scale cloning of cells. Cell culture technology can transform a cell into a simple single cell or a multi-cell with very little differentiation through a large number of cultures. This is an indispensable part of cloning technology, and cell culture itself is the cloning of cells. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation, etc. can be studied. Cell culture bags are needed in the process of cell culture, but most of the existing cell culture bags do not have the effect of increasing cell proliferation capacity in series when used. Therefore, a series cell culture bag is particularly needed.
[0003] However, when using the existing cell culture bags, cells need to proliferate in the non-toxic and sterile cell culture bags. During the cell proliferation, the cell capacity will increase, and the cells need to be transplanted to a new large cell culture bag. During the transplantation process, the skills of the staff are very important. In addition, the cells may be contaminated by bacteria during the transplantation process. Moreover, most of the cell culture bags on the market do not have the function of increasing the capacity of the cell culture bags in a non-transplantable manner, which is very unfavorable for the use of a series cell culture bag. Utility Model Content
[0004] The utility model aims to provide a serial cell culture bag to solve the problem raised in the above background technology. However, in the existing cell culture bags, when in use, cells need to proliferate in the non-toxic and sterile cell culture bag. During the cell proliferation, the capacity of the cells will increase, and the cells need to be transplanted to a new large cell culture bag. During the transplantation process, the skills of the staff are very important. In addition, the cells may be contaminated by bacteria during the transplantation process. Moreover, most of the cell culture bags on the market do not have the function of increasing the capacity of the cell culture bag in a non-transplantable manner, which is very unfavorable for the use of a serial cell culture bag.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a serial cell culture bag, comprising a cell culture bag, the outer surface of the cell culture bag is fixedly connected with an inlet and outlet pipeline, the outer surface of the cell culture bag is fixedly connected with a sampling pipeline, the outer surface of the cell culture bag is provided with a serial volume expansion mechanism, and the outer surface of the cell culture bag is wrapped with a fireproof, anti-corrosion and breathable material;
[0006] The serial capacity expansion mechanism comprises a first convex cap joint, a first concave cap joint, a second concave cap joint, a cell expansion bag and a second convex cap joint. The outer surface of the cell culture bag is fixedly connected with the first convex cap joint, the outer surface of the cell culture bag is fixedly connected with the first concave cap joint, one end surface of the first convex cap joint is snap-connected with the second concave cap joint, one end surface of the second concave cap joint is fixedly connected with the cell expansion bag, and the outer surface of the cell expansion bag is fixedly connected with the second convex cap joint.
[0007] Preferably, the first convex cap joint and the second concave cap joint form a snap-fit structure, and the first concave cap joint and the second convex cap joint form a snap-fit structure.
[0008] Preferably, one end of the first convex cap connector is fixedly connected to the outer surface of the cell culture bag, and the other end of the first convex cap connector is snap-fitted to one end surface of the second concave cap connector.
[0009] Preferably, one end of the first concave cap connector is fixedly connected to the outer surface of the cell culture bag, and the other end of the first concave cap connector is snap-fitted to one end surface of the second convex cap connector.
[0010] Preferably, the fire-proof and anti-corrosion breathable material includes polyethylene, polyester, polypropylene, fluorinated ethylene propylene copolymer and expanded polytetrafluoroethylene. The fire-proof and anti-corrosion breathable material is mixed with polyethylene, the fire-proof and anti-corrosion breathable material is mixed with polyester, the fire-proof and anti-corrosion breathable material is mixed with polypropylene, the fire-proof and anti-corrosion breathable material is mixed with fluorinated ethylene propylene copolymer, and the fire-proof and anti-corrosion breathable material is mixed with expanded polytetrafluoroethylene.
[0011] Compared with the prior art, the utility model has the following beneficial effects: the serial cell culture bag, through the arrangement of the first convex cap joint, the first concave cap joint, the second concave cap joint, the cell expansion bag and the second convex cap joint, when in use, firstly inject cells from the inlet and outlet pipelines, and then inject nutrient solution from the sampling pipeline. During cell culture, the cells will proliferate. At this time, the first convex cap joint and the second concave cap joint are spliced, and then the first concave cap joint and the second convex cap joint are spliced. At this time, the cell expansion bag and the cell culture bag will form a serial passage. When the cells are proliferating, when the volume of the cell culture bag is full, the cells can enter the cell expansion bag. There is no need to transplant the cells a second time. The above arrangement solves the problem in the prior art that, when in use, cells in the existing cell culture bags need to proliferate in the non-toxic and sterile cell culture bags. During the cell proliferation, the cell capacity will increase, and the cells need to be transplanted to a new large cell culture bag. The staff's skills are taken into consideration during the transplantation process, and the cells may be contaminated by bacteria during the transplantation process. Moreover, most of the cell culture bags on the market do not have the function of increasing the capacity of the cell culture bags in a non-transplantable manner, which is very unfavorable for the use of a series cell culture bag. The effect of increasing the cell proliferation capacity in series during cell proliferation is achieved. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 This is a schematic diagram of the appearance structure of the utility model;
[0013] Figure 2 This is a schematic diagram of the structure of the first convex cap joint and the second concave cap joint cooperating with each other in the utility model;
[0014] Figure 3 For this utility model Figure 2 The enlarged structural diagram at A in the middle;
[0015] Figure 4 This is a schematic diagram of the structure of the fireproof, anti-corrosion and breathable material of the utility model.
[0016] In the figure: 1. cell culture bag; 2. inlet and outlet pipes; 3. sampling pipe; 4. series capacity expansion mechanism; 401. first convex cap joint; 402. first concave cap joint; 403. second concave cap joint; 404. cell expansion bag; 405. second convex cap joint; 5. fire-proof, anti-corrosion and breathable material; 501. polyethylene; 502. polyester; 503. polypropylene; 504. fluorinated ethylene propylene copolymer; 505. expanded polytetrafluoroethylene. DETAILED DESCRIPTION
[0017] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0018] See also Figure 1-4 The utility model provides a technical solution: a serial cell culture bag, comprising a cell culture bag 1, an inlet and outlet pipeline 2 is fixedly connected to the outer surface of the cell culture bag 1, a sampling pipeline 3 is fixedly connected to the outer surface of the cell culture bag 1, a serial volume expansion mechanism 4 is arranged on the outer surface of the cell culture bag 1, and a fireproof, anti-corrosion and breathable material 5 is wrapped on the outer surface of the cell culture bag 1;
[0019] The serial capacity expansion mechanism 4 comprises a first convex cap joint 401, a first concave cap joint 402, a second concave cap joint 403, a cell expansion bag 404 and a second convex cap joint 405. The outer surface of the cell culture bag 1 is fixedly connected with the first convex cap joint 401, the outer surface of the cell culture bag 1 is fixedly connected with the first concave cap joint 402, one end surface of the first convex cap joint 401 is clamped and connected with the second concave cap joint 403, one end surface of the second concave cap joint 403 is fixedly connected with the cell expansion bag 404, and the outer surface of the cell expansion bag 404 is fixedly connected with the second convex cap joint 405. 2. The second concave cap joint 403, the cell expansion bag 404 and the second convex cap joint 405 are arranged. When in use, the cells are first injected from the inlet and outlet pipes 2, and then the nutrient solution is injected from the sampling pipe 3. During cell culture, the cells will proliferate. At this time, the first convex cap joint 401 and the second concave cap joint 403 are spliced, and then the first concave cap joint 402 and the second convex cap joint 405 are spliced. At this time, the cell expansion bag 404 and the cell culture bag 1 will form a series passage. When the cells are proliferating, when the volume of the cell culture bag 1 is full, the cells can enter the cell expansion bag 404, and there is no need to transplant the cells for a second time.
[0020] Furthermore, the first convex cap joint 401 and the second concave cap joint 403 form a snap-fit structure, and the first concave cap joint 402 and the second convex cap joint 405 form a snap-fit structure. By setting the first convex cap joint 401, the second concave cap joint 403 can be connected together.
[0021] Furthermore, one end of the first convex cap joint 401 is fixedly connected to the outer surface of the cell culture bag 1, and the other end of the first convex cap joint 401 is snap-fitted to one end surface of the second concave cap joint 403. Through the arrangement of the first convex cap joint 401 and the second concave cap joint 403, a passage can be formed between the cell culture bag 1 and the cell expansion bag 404.
[0022] Furthermore, one end of the first concave cap joint 402 is fixedly connected to the outer surface of the cell culture bag 1, and the other end of the first concave cap joint 402 is snap-fitted to one end surface of the second convex cap joint 405. Through the arrangement of the first concave cap joint 402 and the second convex cap joint 405, a passage can be formed between the cell culture bag 1 and the cell expansion bag 404.
[0023] Further, the fireproof and anti-corrosion breathable material 5 includes polyethylene 501, polyester 502, polypropylene 503, fluorinated ethylene propylene copolymer 504 and expanded polytetrafluoroethylene 505, the fireproof and anti-corrosion breathable material 5 is mixed with polyethylene 501, the fireproof and anti-corrosion breathable material 5 is mixed with polyester 502, the fireproof and anti-corrosion breathable material 5 is mixed with polypropylene 503, the fireproof and anti-corrosion breathable material 5 is mixed with fluorinated ethylene propylene copolymer 504, the fireproof and anti-corrosion breathable material 5 is mixed with expanded polytetrafluoroethylene 505, and the fireproof and anti-corrosion breathable material 5 is mixed with polyethylene 501, polyester 502, polypropylene 503, fluorinated ethylene propylene copolymer 504, and the fireproof and anti-corrosion breathable material 5 is mixed with expanded polytetrafluoroethylene 505. The fluorinated ethylene propylene copolymer 504 and the expanded polytetrafluoroethylene 505 are arranged. When in use, firstly the polyethylene 501 is odorless and non-toxic, which can increase the low temperature resistance and the corrosion resistance of most acids and alkalis of the cell culture bag 1; then the polyester 502 is odorless and non-toxic, which can increase the elasticity of the cell culture bag 1; then the polypropylene 503 is odorless and non-toxic, which can increase the chemical resistance, heat resistance and electrical insulation of the cell culture bag 1; the fluorinated ethylene propylene copolymer 504 can increase the air permeability and waterproofness of the cell culture bag 1; the expanded polytetrafluoroethylene 505 can increase the durability, waterproofness, elasticity and flexibility of the cell culture bag 1.
[0024] Working principle: First, inject cells from the inlet and outlet pipes 2, and then inject nutrient solution from the sampling pipe 3. During cell culture, cells will proliferate. At this time, the first convex cap joint 401 and the second concave cap joint 403 are spliced, and then the first concave cap joint 402 and the second convex cap joint 405 are spliced. At this time, the cell expansion bag 404 and the cell culture bag 1 will form a series passage. When the cell culture bag 1 is full, the cells can enter the cell expansion bag 404. The cells need to be transplanted a second time. First, polyethylene 501 is odorless and non-toxic, which can increase the low temperature resistance and resistance to most acid and alkali corrosion of the cell culture bag 1. Then polyester 502 is odorless and non-toxic, which can increase the elasticity of the cell culture bag 1. Then polypropylene 503 is odorless and non-toxic, which can increase the chemical resistance, heat resistance and electrical insulation of the cell culture bag 1. Fluorinated ethylene propylene copolymer 504 can increase the air permeability and waterproofness of the cell culture bag 1. Expanded polytetrafluoroethylene 505 can increase the durability, waterproofness, elasticity and flexibility of the cell culture bag 1.
[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A serial cell culture bag, comprising a cell culture bag (1), characterized in that: The outer surface of the cell culture bag (1) is fixedly connected to an inlet and outlet pipeline (2), the outer surface of the cell culture bag (1) is fixedly connected to a sampling pipeline (3), the outer surface of the cell culture bag (1) is provided with a series volume expansion mechanism (4), and the outer surface of the cell culture bag (1) is wrapped with a fireproof, anti-corrosion and breathable material (5); The serial volume expansion mechanism (4) comprises a first convex cap joint (401), a first concave cap joint (402), a second concave cap joint (403), a cell volume expansion bag (404) and a second convex cap joint (405); the outer surface of the cell culture bag (1) is fixedly connected to the first convex cap joint (401); the outer surface of the cell culture bag (1) is fixedly connected to the first concave cap joint (402); one end surface of the first convex cap joint (401) is snap-connected to the second concave cap joint (403); one end surface of the second concave cap joint (403) is fixedly connected to the cell volume expansion bag (404); and the outer surface of the cell volume expansion bag (404) is fixedly connected to the second convex cap joint (405).
2. A serial cell culture bag according to claim 1, characterized in that: The first convex cap joint (401) and the second concave cap joint (403) form a snap-fit structure, and the first concave cap joint (402) and the second convex cap joint (405) form a snap-fit structure.
3. A serial cell culture bag according to claim 1, characterized in that: One end of the first convex cap connector (401) is fixedly connected to the outer surface of the cell culture bag (1), and the other end of the first convex cap connector (401) is snap-fitted to one end surface of the second concave cap connector (403).
4. The serial cell culture bag according to claim 1, characterized in that: One end of the first concave cap joint (402) is fixedly connected to the outer surface of the cell culture bag (1), and the other end of the first concave cap joint (402) is snap-fitted to one end surface of the second convex cap joint (405).
5. The serial cell culture bag according to claim 1, characterized in that: The fireproof and anti-corrosion breathable material (5) comprises polyethylene (501), polyester (502), polypropylene (503), fluorinated ethylene propylene copolymer (504) and expanded polytetrafluoroethylene (505); the fireproof and anti-corrosion breathable material (5) is mixed with polyethylene (501); the fireproof and anti-corrosion breathable material (5) is mixed with polyester (502); the fireproof and anti-corrosion breathable material (5) is mixed with polypropylene (503); the fireproof and anti-corrosion breathable material (5) is mixed with fluorinated ethylene propylene copolymer (504); and the fireproof and anti-corrosion breathable material (5) is mixed with expanded polytetrafluoroethylene (505).