Centering tool for flow type pool

Abstract

The utility model provides a flow type pool centering tool. The flow type pool centering tool comprises an adjusting platform, a light source and a camera. The adjusting platform is provided with a mounting position for bearing the flow type pool and a light-transmitting window; the light source is located on one side of the adjusting platform and used for irradiating the flow type pool on the adjusting platform, so that light penetrating through a first through hole in the flow type pool forms a first light spot, and light penetrating through a second through hole in the waste liquid connector forms a second light spot; the camera is used for collecting images of the first light spot and the second light spot respectively, and the images are used for indicating whether the flow type pool and the waste liquid connector are installed in place or not. According to the flow type pool centering tool, the adjusting platform is used for bearing the flow type pool to be centered, the camera is used for collecting light spots formed when the light source irradiates the flow type pool and the waste liquid connector, the geometric center of the first light spot and the geometric center of the second light spot are compared, and the position deviation of the waste liquid connector and the flow type pool can be obtained; in this way, centering of the flow type pool and the waste liquid connector can be conveniently and accurately completed manually or by a machine.

Description

Technical Field

[0001] The present application relates to the technical field of nanometer flow cytometers, and in particular to a flow cell centering tool. Background Art

[0002] In the existing general flow industry, the assembly of the flow pool is a key process. When judging whether the flow pool assembly meets the requirements, the operator usually judges by visual laminar flow. The adjustment and installation steps are roughly as follows:

[0003] First, pre-install the flow cell, fix it to the customized tooling and connect the liquid system. Then use the camera to observe whether the laminar flow in the flow cell is stable and centered; if the laminar flow is not ideal, manually fine-tune the flow cell docking point up and down and left and right, and simultaneously observe whether the laminar flow improves. Repeat this step until the laminar flow is stable and centered (visual judgment), and finally lock the flow cell, and the debugging and assembly are completed. The judgment result is relatively subjective; and when different operators operate, because there is no quantitative data to judge, it is usually difficult to ensure consistency, and the repeatability of production cannot be guaranteed. Utility Model Content

[0004] In view of this, the embodiment of the present application provides a flow cell centering tool to solve the problem that the solution for judging whether the flow cell is installed in place in the background art is relatively subjective.

[0005] The present application provides a flow cell centering tool, comprising:

[0006] An adjustment platform having a mounting position for carrying the flow cell and a light-transmitting window;

[0007] a light source, located at one side of the adjustment platform, and used for irradiating the flow cell on the adjustment platform so that the light transmitted through the first through hole on the flow cell forms a first light spot, and the light transmitted through the second through hole on the waste liquid connector forms a second light spot;

[0008] A camera is located on opposite sides of the adjustment platform from the light source, and is used to collect images of the first light spot and the second light spot respectively, and the images are used to indicate whether the flow cell and the waste liquid connector are installed in place.

[0009] In an optional embodiment, the flow cell centering tool further includes an adjustment mechanism, wherein the adjustment mechanism is adjacent to the adjustment platform and can contact the flow cell to adjust the position of the flow cell.

[0010] In an optional embodiment, the adjustment mechanism includes:

[0011] First base;

[0012] An adjusting rod is movably connected to the first base, the adjusting rod can move along its own axis, and the end of the adjusting rod is used to push the flow cell on the adjusting platform.

[0013] In an optional embodiment, the adjustment rod is a measuring rod of a micrometer.

[0014] In an optional embodiment, the adjustment mechanism also includes: a movable pusher head and a second base, the movable pusher head can be movably connected to the second base, the movable pusher head and the adjustment rod are respectively located on two opposite sides of the adjustment platform, and the movable pusher head is used to push the flow pool pushed by the adjustment rod in the opposite direction.

[0015] In an optional embodiment, at least two adjusting rods are provided, at least one of the adjusting rods is axially arranged along a first direction, and at least one of the adjusting rods is axially arranged along a second direction, wherein the first direction and the second direction intersect.

[0016] In an optional embodiment, the flow cell centering tool further includes a camera adjustment assembly connected to the camera.

[0017] In an optional embodiment, the camera adjustment assembly includes a camera connecting part and a three-axis movable frame, the three-axis movable frame is connected to the camera connecting part, the camera connecting part is fixed to the camera, and the camera connecting part can drive the camera to move along the X-axis, Y-axis and Z-axis directions in the spatial coordinate system respectively under the action of the three-axis movable frame.

[0018] In an optional embodiment, the flow cell centering tool further includes an optical platform and a machine table, the upper surface of the optical platform is horizontal, and the three-axis moving frame is connected to the upper surface of the optical platform;

[0019] The adjustment platform is installed on the machine platform, and the machine platform is located between the adjustment platform and the optical platform, and the camera is located below the adjustment platform.

[0020] In an optional implementation, the camera is a black and white camera.

[0021] In an optional implementation, the camera includes a telecentric lens, and the light source is a parallel light source.

[0022] In an optional embodiment, the flow cell centering tool further comprises:

[0023] A display device is connected to the camera for communication, and the display device is used to display the image.

[0024] The flow cell alignment tool provided in the embodiment of the present application carries the flow cell to be aligned through an adjustable platform, and uses a camera to capture the light spot formed by the light source irradiating the flow cell and the waste liquid connector. By comparing the geometric centers of the first light spot and the second light spot, the position deviation of the waste liquid connector and the flow cell can be obtained, thereby facilitating manual or machine alignment of the flow cell and the waste liquid connector more accurately.

[0025] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0027] Figure 1 A schematic diagram of the flow cell centering tooling structure provided in an embodiment of the present application;

[0028] Figure 2 A schematic diagram of the arrangement position of the adjustment mechanism provided in the embodiment of the present application;

[0029] Figure 3 A schematic diagram of the structure of the adjustment mechanism provided in the embodiment of the present application;

[0030] Figure 4 A schematic diagram of the connection relationship between the flow cell and the waste liquid connector provided in the embodiment of the present application;

[0031] Figure 5 A schematic diagram of the relative relationship between the first light spot and the second light spot provided in an embodiment of the present application;

[0032] Figure 6 A schematic diagram of the structure of a camera adjustment assembly provided in an embodiment of the present application;

[0033] Figure 7 A schematic diagram of the flow cell centering method provided in an embodiment of the present application.

[0034] 1. Adjust the platform;

[0035] 2. Light source;

[0036] 3. Camera;

[0037] 4. Flow cell; 41. first through hole;

[0038] 5. Waste liquid connector; 51. Second through hole;

[0039] 6. Adjustment mechanism; 61. First base; 62. Adjustment rod; 63. Movable push head; 64. Second base;

[0040] 7. Camera adjustment assembly; 71. Camera connection part; 72. Three-axis moving frame;

[0041] 8. Optical platform;

[0042] 9. Machine;

[0043] 101. First light spot; 102. Second light spot; 103. Reference line. DETAILED DESCRIPTION

[0044] The exemplary embodiments disclosed in the present application will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present application are shown in the accompanying drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the specific embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope disclosed in the present application to those skilled in the art.

[0045] In the following description, a large number of specific details are given to provide a more thorough understanding of the present application. However, it is obvious to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features known in the art are not described; that is, all features of the actual embodiments are not described here, and well-known functions and structures are not described in detail.

[0046] In the drawings, the sizes of components, mechanisms, gaps, images and their relative sizes may be exaggerated for clarity. Like reference numerals denote like elements throughout.

[0047] It should be understood that when a component or mechanism is referred to as "on ...", "adjacent to ...", "connected to" or "coupled to" other components or mechanisms, it can be directly on, adjacent to, connected to or coupled to other components or mechanisms, or there can be intermediate components or mechanisms. On the contrary, when a component or mechanism is referred to as "directly on ...", "directly adjacent to ...", "directly connected to" or "directly coupled to" other components or mechanisms, there is no intermediate component or mechanism. It should be understood that although the terms first, second, third, etc. can be used to describe various components, mechanisms, gaps and / or parts, these components, mechanisms, gaps and / or parts should not be limited by these terms. These terms are only used to distinguish one component, mechanism, gap or part from another component, mechanism, gap or part. Therefore, without departing from the teachings of the present application, the first component, mechanism, gap or part discussed below can be expressed as the second component, mechanism, gap or part. And when the second component, mechanism, gap or part is discussed, it does not indicate that the present application necessarily has the first component, mechanism, gap or part.

[0048] Spatially relative terms such as "under", "beneath", "below", "under", "above", "above", etc., may be used herein for convenience of description to describe the relationship of one component or feature shown in the figures to other components or features. It should be understood that the spatially relative terms are intended to include different orientations of the device in use and operation in addition to the orientations shown in the figures. For example, if the device in the drawings is turned over, then the components or features described as "under the other components" or "beneath" or "below" will be oriented as "above" the other components or features. Therefore, the exemplary terms "under" and "under" may include both upper and lower orientations. The components may be oriented otherwise (rotated 90 degrees or other orientations) and the spatial descriptors used herein are interpreted accordingly.

[0049] The purpose of the terms used herein is only to describe specific embodiments and is not intended to be limiting of the present application. When used herein, the singular forms "one", "an" and "said / the" are also intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "consisting of" and / or "comprising", when used in this specification, determine the presence of the features, integers, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, parts and / or groups. When used herein, the term "and / or" includes any and all combinations of the relevant listed items.

[0050] In order to thoroughly understand the present application, detailed steps and detailed structures will be presented in the following description to illustrate the technical solution of the present application. The preferred embodiments of the present application are described in detail below, but in addition to these detailed descriptions, the present application may also have other implementation methods.

[0051] The present application embodiment provides a flow cell centering tool, such as Figure 1-Figure 4 As shown, it includes: an adjustment platform 1, a light source 2, and a camera 3.

[0052] The adjustment platform 1 has a mounting position for carrying the flow cell 4 and a light-transmitting window. The light source 2 is used to illuminate the flow cell 4 on the adjustment platform 1, so that the light transmitted through the first through hole 41 on the flow cell 4 forms a first light spot 101, and the light transmitted through the second through hole 51 on the waste liquid connector 5 forms a second light spot 102. The camera 3 is used to receive images of the first light spot 101 and the second light spot 102.

[0053] The centering mentioned in this embodiment refers to the alignment of the centers of the first through hole 41 and the second through hole 51, wherein the first through hole 41 and the second through hole 51 are holes that the flow cell 4 and the waste liquid connector 5 need to be docked. Only after the two are docked accurately can the laminar flow of the liquid path system be clearly observed, and the accurate docking of the two can also indicate that the flow cell 4 and the waste liquid connector 5 are installed in place. The flow cell 4 of this embodiment has a first through hole 41, which transmits light under the irradiation of the light source 2, and forms a first light spot 101 on the opposite side of the light source 2. Therefore, the shape of the first light spot 101 reflects the shape of the first through hole 41, and the position of the first light spot 101 also reflects the position of the first through hole 41. Similarly, the waste liquid connector 5 of this embodiment has a second through hole 51, which transmits light under the irradiation of the light source 2, and forms a second light spot 102 on the opposite side of the light source 2. Therefore, the shape of the second light spot 102 reflects the shape of the second through hole 51, and the position of the second light spot 102 also reflects the position of the second through hole 51.

[0054] Camera 3 is used to receive images of the first light spot 101 and the second light spot 102, and the images are used to indicate whether the flow cell 4 and the waste liquid connector 5 are installed in place; the image information received by camera 3 can be recorded and calculated by a computer, and the image information may include but is not limited to size, shape, position, etc. Since the image information collected by camera 3 is composed of specific pixels, the image can be magnified, and the image information of the first light spot 101 and the second light spot 102, especially the shape and position, is more objective than the operator's naked eye observation, so the operator can adjust the position of the flow cell 4 according to the image information collected by camera 3, which is more controllable and objective. In order to facilitate the camera 3 to collect light, such as Figure 1 As shown, the camera 3 is arranged below the adjustment platform 1, and the light source 2 is arranged above the adjustment platform 1. It can be understood that except Figure 1 In the position shown, the camera 3 can also be arranged above the adjustment platform 1 and the light source 2 can be arranged below the adjustment platform 1 .

[0055] The collected spot image information mentioned in this embodiment can also be regarded as collecting edge information of the holes of the flow cell 4 and the waste liquid connector 5, because the first through hole 41 and the second through hole 51 are light-transmissive, forming a bright spot in the image collected by the camera, while the periphery of the first through hole 41 and the second through hole 51 is light-impermeable, forming a dark part in the image collected by the camera. That is, the light-dark dividing line of the image collected by the camera is the edge of the hole.

[0056] The adjustment platform 1 has a mounting position for carrying the flow cell 4 and a light-transmitting window. On the one hand, it is convenient to carry the flow cell 4. On the other hand, when the flow cell 4 and the waste liquid connector 5 are placed thereon, the light-transmitting window designed thereon can be aligned with the first through hole 41 and the second through hole 51, so that when the light source 2 irradiates the flow cell 4 and the waste liquid connector 5, the light spots transmitted through the first through hole 41 and the second through hole 51 can be collected by the camera 3 on the opposite side through the light-transmitting window. The light-transmitting window can be a hollow formed on the adjustment platform 1, or it can be a window made of a transparent material installed on the adjustment platform 1.

[0057] The flow cell centering tool provided in the embodiment of the present application carries the flow cell 4 to be aligned by adjusting the platform 1, and uses the camera 3 to collect the light spot formed by the light source 2 irradiating the flow cell 4 and the waste liquid connector 5. By comparing the geometric centers of the first light spot 101 and the second light spot 102, the position deviation of the waste liquid connector 5 and the flow cell 4 can be obtained, thereby facilitating manual or machine alignment of the flow cell 4 and the waste liquid connector 5 more accurately.

[0058] In this embodiment, the flow cell centering tool further includes a display device, through which the positions or relative positions of the first light spot 101 and the second light spot 102 are displayed. In this embodiment, the second through hole 51 of the waste liquid connector 5 is circular in shape, and the first through hole 41 of the flow cell 4 is rectangular in shape. Figure 5 As shown, the first light spot 101 is a rectangle, the second light spot 102 is a circle, and the edge of the circle is the reference line 103 of the display interface. Through the shape and position of the light spots displayed on the display interface, the operator can easily see the position deviation of the first light spot 101 and the second light spot 102 according to the coordinates of the geometric center of the light spots, wherein the area within the reference line 103 is the range of the software recognition image.

[0059] It is understandable that, in order to know the shape and position of the light spot, in addition to the display device, in other embodiments, a method such as voice broadcasting the position or relative position of the first light spot 101 and the second light spot 102 can also be used. Or if the machine is automatically adjusted, in order to know the shape and position of the light spot, a processor can also be used to calculate the position or relative position of the first light spot 101 and the second light spot 102. In this embodiment, the display device is connected to the camera 3 for communication, and the display device includes a display screen and a processor. The processor recognizes and calculates the captured image, and sends the processed result to the display screen for display.

[0060] In an optional embodiment, if Figure 2 As shown, the flow pool centering tool also includes an adjustment mechanism 6, which is adjacent to the adjustment platform 1 and can contact the flow pool 4 to adjust the position of the flow pool 4. The arrangement of the adjustment mechanism 6 can facilitate multi-directional adjustment of the position of the flow pool 4 around the adjustment platform 1, so as to quickly adjust the flow pool 4 into place. Figure 2 As shown, a machine platform 9 can be provided, and the adjustment mechanism 6 can be mounted on the machine platform 9 .

[0061] In an optional embodiment, if Figure 2 , Figure 3 As shown, the adjustment mechanism 6 includes: a first base 61 and an adjustment rod 62, the adjustment rod 62 is movably connected to the first base 61, the adjustment rod 62 can move along its own axial direction, and its end is used to push the flow pool 4 on the adjustment platform 1. Through the setting of the adjustment rod 62, the movement of the adjustment rod 62 along the axial direction can be used to drive the flow pool 4 to move, and the controllability of pushing the flow pool 4 is higher.

[0062] In an optional embodiment, the adjustment rod 62 is a micrometer rod. In this embodiment, the structure of the micrometer rod is used as the adjustment rod 62, so that the position of the flow cell 4 can be adjusted with high precision, so as to reduce the offset error when adjusting the flow cell 4 and improve the accuracy of the centering.

[0063] In an optional embodiment, the adjustment mechanism 6 further includes: a movable push head 63 and a second base 64, the movable push head 63 is movably connected to the second base 64, the movable push head 63 and the adjustment rod 62 are respectively located on opposite sides of the adjustment platform 1, and the movable push head 63 is used to push the flow pool 4 pushed by the adjustment rod 62 in the opposite direction. In this embodiment, the function of the movable push head 63 is to push the adjusted flow pool 4 back. Since the adjustment rod 62 can only push the flow pool 4, once the flow pool 4 is pushed too far, the operator can only manually push the flow pool 4 back, which is inconvenient to operate. Therefore, the movable push head 63 in this embodiment can improve the convenience of operation in this case.

[0064] In an optional embodiment, if Figure 3 As shown, two adjusting rods 62 and the first base 61 are provided, one is provided along the X-axis, and the other is provided along the Y-axis, so that the adjusting rod 62 can quickly adjust the position of the flow pool 4 in both the X-axis and the Y-axis directions. The above-mentioned X-axis and Y-axis are two axes defined in the horizontal direction based on the Cartesian coordinate system, and the other Z-axis is along the vertical direction. It can be understood that in other embodiments, three, four or more adjusting rods 62 and the first base 61 can be provided, and the direction of the provision is not limited to the X-axis and the Y-axis.

[0065] In an optional embodiment, if Figure 1 As shown, the flow cell centering tool further includes a camera adjustment component 7 connected to the camera 3. The camera adjustment component 7 can be arranged to adjust the position of the camera 3 to facilitate the camera 3 to complete the alignment and focus of the flow cell 4 and the waste liquid connector 5.

[0066] In an optional embodiment, if Figure 6 As shown, the camera adjustment assembly 7 includes a camera connection portion 71 and a three-axis moving frame 72. The three-axis moving frame 72 is connected to the camera connection portion 71 and is used to drive the camera connection portion 71 to move in three axes. The camera connection portion 71 is fixed to the camera 3. Under the action of the three-axis moving frame 72, the camera connection portion 71 can drive the camera 3 to move along the X-axis, Y-axis and Z-axis directions in the spatial coordinate system. The three-axis moving frame 72 in this embodiment is an existing product, and its characteristic is that it can adjust the camera 3 on the X-axis, Y-axis and Z-axis so that the object to be measured is located at the focus of the camera 3 field of view. The setting of the camera connection portion 71 can facilitate the fixing of the camera 3, so that the camera 3 can move smoothly with the three-axis moving frame 72.

[0067] In an optional embodiment, if Figure 6 As shown, the flow cell centering tooling also includes an optical platform 8, the upper surface of the optical platform 8 is horizontal, and the three-axis moving frame 72 is connected to the upper surface of the optical platform 8; Figure 1 , Figure 2 As shown, the adjustment platform 1 is installed on the machine table 9, and the machine table 9 is located between the adjustment platform 1 and the optical platform 8, and the camera 3 is located below the adjustment platform 1. The upper surface of the optical platform 8 is optically positioned to ensure that it is level, so as to ensure that the X-axis, Y-axis and Z-axis of the three-axis moving frame 72 are relatively accurate when they are adjusted, so as to ensure the accuracy of the image captured by the camera 3.

[0068] In an optional embodiment, the camera 3 is a black and white camera. Since the camera 3 is only required to obtain the image of the light spot in this embodiment, a simpler image can be obtained by using a black and white camera, which is sufficient for judging parameters such as image shape and position. The black and white camera has a lower cost and is conducive to industrialization.

[0069] In an optional embodiment, the camera 3 includes a telecentric lens, and the light source 2 is a parallel light source, so that the edge of the flow cell 4 can be imaged clearly in the camera 3 with little deformation.

[0070] This embodiment provides a flow cell centering method using the above-mentioned flow cell centering tooling, such as Figure 7 As shown, the following steps are included:

[0071] 1. Open the flow cell alignment software. After the software is started, the software initializes the image memory, reads the configuration file and automatically connects to camera 3 (CCD), and sets the exposure time, trigger mode and other parameters;

[0072] 2. Adjust the Z-axis height of camera 3 (CCD) to find the clearest circular spot position until the software recognizes the circular spot, collect and obtain single-frame image data, and use the image recognition algorithm to determine the center position of the circular spot;

[0073] 3. Adjust the Z-axis height of the camera 3 (CCD) to find the clearest rectangular spot position until the software recognizes the rectangular spot, collects and acquires single-frame image data, and uses the image recognition algorithm to determine the center position of the rectangular spot. Use the software to calculate the geometric center reference deviation value of the rectangular spot and the circular spot, and judge whether it is qualified or unqualified according to the set acceptance standard. If it is unqualified, the operator adjusts the adjustment mechanism 6 according to the reference deviation value to make the deviation value <5um.

[0074] During this process, the benchmark deviation value can be displayed on the display screen. The display screen can also be used to display the light spot image magnified by the software or the cross cursor of the geometric center of the rectangular light spot and the circular light spot generated by the software. For example, a green cross cursor is generated at the geometric center of the rectangular light spot, and a red cross cursor is generated at the geometric center of the circular light spot, so that the operator can watch and check.

[0075] The flow cell centering method provided in this embodiment automates the measurement of deviations, improves debugging efficiency, and is beneficial to quality control; it realizes automated judgment, simplifies the debugging steps, realizes the quantification of deviations, eliminates human subjective judgment, and is beneficial to the guarantee of repeatability after production.

[0076] This embodiment provides a step of aligning the waste liquid connector 5 with the flow cell 4:

[0077] 1. Preparation

[0078] Fix the waste liquid connector 5 to the external device, and be careful not to shield the light source 2 from the external device during installation; install a 3mm O-ring on the flow cell 4, press it in with tweezers, and make sure that the O-ring is embedded in the groove at one end of the first through hole 41, and the O-ring is flush without irregular protrusions. Remove the lampshade on the adjustment platform 1, install the flow cell 4 on the adjustment platform 1, tighten the four screws around the adjustment platform 1, and fix it to the machine 9;

[0079] Place the flow cell 4 under the waste liquid connector 5, use tweezers to adjust the position of the flow cell 4, align the assembly holes of the two after visual alignment, and use the screw 52 (M3x16) with a spring washer to slightly lock the flow cell 4 to facilitate the adjustment of the position of the flow cell 4;

[0080] Start the computer program and run the flow cell centering fixture software. After the software is started, the software automatically connects to the camera 3 and sets the exposure time, trigger mode and other parameters. The software mentioned in this embodiment is the existing public software.

[0081] 2. Image acquisition stage

[0082] Turn on the light source 2, put back the lampshade, adjust the height of the vertical axis of the camera 3 (the other axes cannot be adjusted), observe the display screen, and when the clearest circular light spot (i.e., the second light spot 102) is found, the camera 3 stops adjusting, and the software is used to collect the image of the circular light spot at this time. At this time, the center position of the circular light spot is determined, and this position is recorded as the first position (the recording method includes but is not limited to coordinate representation or vector representation);

[0083] Adjust the height of the vertical axis of the camera 3 again (the other axes cannot be adjusted), observe the display screen, and when the clearest rectangular light spot (i.e., the first light spot 101) is found, the camera 3 stops adjusting, and the software is used to collect the image of the rectangular light spot at this time. At this time, the geometric center position of the rectangular light spot is determined, and this position is recorded as the second position (the recording method includes but is not limited to coordinate representation or vector representation);

[0084] Based on the first position and the second position, the required adjustment amount of the convection pool 4 is obtained by the software.

[0085] In the above process, the function of adjusting the vertical height of the camera 3 is to change the focus. Since the first through hole 41 and the second through hole 51 are located at different focal lengths, the vertical height of the camera 3 can be adjusted from focusing on the first through hole 41 to focusing on the second through hole 52. When collecting images, the first light spot 101 and the second light spot 102 can be collected in any order.

[0086] 3. Adjustment stage

[0087] Push the flow cell 4 while keeping the display screen on until the geometric centers of the rectangular light spot and the circular light spot are aligned. If necessary, the image can be enlarged, or the geometric centers of the rectangular light spot and the circular light spot can be displayed with different cursors through software for alignment. When the geometric center deviation of the rectangular light spot and the circular light spot is less than 5um, the screws of the waste liquid connector 5 can be tightened, and the two screws can be rotated in turn to avoid causing the flow cell 4 to move.

[0088] Use a torque wrench (M3) to tighten the two set screws of the waste liquid connector 5. Clamp one side of the flow cell 4 with plastic tweezers and gently turn it to confirm whether the flow cell 4 is pressed tightly and cannot be moved. If it can be moved, screw the waste liquid connector 5 down until the flow cell 4 cannot be moved, and confirm whether the offset value still meets the requirements. If not, loosen the screws of the waste liquid connector 5 and readjust it, and repeat the above steps.

[0089] After the adjustment is completed, loosen the four screws that fix the adjustment platform 1 on the machine table 9, remove the adjustment platform 1 and its accessories, and the debugging and installation are completed.

[0090] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present application that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present application and do not limit the scope of protection of the patent of this application.

Claims

1. A flow cell centering tool, characterized in that: include: An adjustment platform (1) having a mounting position for carrying the flow cell (4) and a light-transmitting window; A light source (2) is located on one side of the adjustment platform (1), and the light source (2) is used to illuminate the flow cell (4) on the adjustment platform (1), so that light transmitted through a first through hole (41) on the flow cell (4) forms a first light spot (101), and light transmitted through a second through hole (51) on the waste liquid connector (5) forms a second light spot (102); A camera (3) is located on opposite sides of the adjustment platform (1) from the light source (2), and the camera (3) is used to respectively capture images of the first light spot (101) and the second light spot (102), and the images are used to indicate whether the flow cell (4) and the waste liquid connector (5) are installed in place.

2. The flow cell centering tooling according to claim 1, characterized in that: The flow cell centering tool further comprises an adjustment mechanism (6), wherein the adjustment mechanism (6) is adjacent to the adjustment platform (1) and is capable of contacting the flow cell (4) to adjust the position of the flow cell (4).

3. The flow cell centering tooling according to claim 2, characterized in that: The regulating mechanism (6) comprises: A first base (61); The adjusting rod (62) is movably connected to the first base (61); the adjusting rod (62) can move along its own axis; and the end of the adjusting rod (62) is used to push the flow cell (4) on the adjusting platform (1).

4. The flow cell centering tool according to claim 3, characterized in that: The adjusting rod (62) is a measuring rod of a micrometer.

5. The flow cell centering tool according to claim 3, characterized in that: The adjustment mechanism (6) further comprises: a movable pusher head (63) and a second base (64); the movable pusher head (63) is movably connected to the second base (64); the movable pusher head (63) and the adjustment rod (62) are respectively located on two opposite sides of the adjustment platform (1); the movable pusher head (63) is used to push the flow pool (4) pushed by the adjustment rod (62) in the opposite direction.

6. The flow cell centering tool according to any one of claims 3 to 5, characterized in that: At least two adjusting rods (62) are provided, at least one of the adjusting rods (62) is axially arranged along a first direction, and at least one of the adjusting rods (62) is axially arranged along a second direction, wherein the first direction and the second direction intersect.

7. The flow cell centering tool according to claim 1, characterized in that: The flow cell centering tool further comprises a camera adjustment component (7) connected to the camera (3).

8. The flow cell centering tool according to claim 7, characterized in that: The camera adjustment assembly (7) comprises a camera connecting portion (71) and a three-axis movable frame (72), wherein the three-axis movable frame (72) is connected to the camera connecting portion (71), the camera connecting portion (71) is fixed to the camera (3), and the camera connecting portion (71) can drive the camera (3) to move respectively along the X-axis, Y-axis and Z-axis directions in the space coordinate system under the action of the three-axis movable frame (72).

9. The flow cell centering tool according to claim 8, characterized in that: The flow cell centering tooling also includes an optical platform (8) and a machine table (9), the upper surface of the optical platform (8) is horizontal, and the three-axis moving frame (72) is connected to the upper surface of the optical platform (8); The adjustment platform (1) is installed on the machine platform (9), and the machine platform (9) is located between the adjustment platform (1) and the optical platform (8), and the camera (3) is located below the adjustment platform (1).

10. The flow cell centering tool according to any one of claims 1 to 5, characterized in that: The flow cell centering tool also includes: A display device is communicatively connected to the camera (3), and the display device is used to display the image.

Images