Antibody purification device

Abstract

The utility model discloses an antibody purification device, which belongs to the technical field of antibody purification, and adopts the technical scheme that the antibody purification device comprises a connecting and positioning mechanism, a clamping component, an injection component, a chromatographic column and a main body, the connecting and positioning mechanism comprises a connecting component and a positioning component, and the connecting component and the positioning component are connected through the connecting component. The connecting assembly can achieve the effect of connecting the positioning assembly, the clamping assembly and the injection assembly, meanwhile, the connecting assembly can also achieve the effect of limiting the positioning assembly, and through the arrangement of the positioning assembly, the positioning assembly is matched with the connecting assembly for use, the effect of positioning and fixing the chromatographic column can be achieved; the purification device is provided with the clamping assembly and the injection assembly, the clamping assembly can achieve the effect of connecting the connecting pipe on the purification device to the chromatographic column, and the injection assembly can achieve the effect of injecting eluent into the chromatographic column through the arrangement of the injection assembly.

Description

Technical Field

[0001] The utility model relates to the technical field of antibody purification, in particular to an antibody purification device. Background Art

[0002] An antibody is a large Y-shaped protein secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. It is only found in the blood and other body fluids of vertebrates and on the cell membrane surface of their B cells. Antibodies can recognize a unique feature of a specific foreign object, which is called an antigen.

[0003] At present, the Chinese utility model with the announcement number: CN215855858U discloses a biomedical technology device for rapid purification of trace amounts of antibodies, including a substrate and reaction chamber A, reaction chamber B, liquid inlet channel A, liquid inlet channel B, liquid outlet channel, liquid inlet filter element, liquid outlet filter element, intermediate channel, etc. in the substrate. This device only requires a trace amount of stock solution to separate and extract high-purity trace antibodies therefrom, and is simple to operate and fast, does not require expensive instruments and equipment, and greatly saves purification time.

[0004] When making antibodies, an antibody purification device is needed to purify the antibodies. Most of the existing antibody purification methods use chromatography column purification. However, after the chromatography column is fixed on the purification device, this method often requires manual connection of the pipeline on the purification device to the chromatography column, which is more troublesome. In the purification process, eluent needs to be added for neutralization. However, most of the existing methods of adding eluent are to manually use a dropper to draw the eluent and then drip the eluent in the dropper into the chromatography column according to the required approximate amount, which is inconvenient. Utility Model Content

[0005] The utility model provides an antibody purification device, aiming to solve the existing antibody purification methods, most of which adopt a chromatography column purification method. However, after the chromatography column is fixed on the purification device, this method often requires manual connection of the pipeline on the purification device to the chromatography column, which is relatively troublesome. In addition, during the purification process, an eluent needs to be added for neutralization treatment. However, the existing method of adding the eluent is mostly to manually use a dropper to draw the eluent and then drip the eluent in the dropper into the chromatography column according to the required approximate amount, which is an inconvenience.

[0006] The utility model is implemented as follows: an antibody purification device comprises a connection positioning mechanism, a clamping assembly, an injection assembly, a chromatography column and a main body, wherein the connection positioning mechanism is arranged at the front side of the main body, the clamping assembly is arranged at the bottom of the connection positioning mechanism, the injection assembly is arranged at the top of the connection positioning mechanism, and the chromatography column is arranged at the front side of the connection positioning mechanism;

[0007] The connection and positioning mechanism comprises a connection component and a positioning component. The connection component is arranged at the front side of the main body, and the positioning component is arranged at the front side of the connection component.

[0008] In order to enable the connecting component to achieve the effect of connecting the positioning component, the locking component and the injection component, and at the same time the connecting component can also play the effect of limiting the positioning component, as a preferred antibody purification device of the utility model, the connecting component includes a positioning plate, a locking plate, an adjustment groove and a rotating hole, the positioning plate is fixedly connected to the front side of the main body, the locking plate is fixedly connected to the top of the front side of the positioning plate, the adjustment groove is opened on the front side of the positioning plate, and the rotating hole is opened on both sides of the positioning plate.

[0009] In order to make the positioning component cooperate with the connecting component to achieve the effect of positioning and fixing the chromatography column, and then facilitate the use of the clamping component and the injection component, as a preferred antibody purification device of the utility model, the positioning component includes an adjusting rod, an anti-slip pad, an adjusting plate, a motor and a splint, the adjusting rod is threadedly connected to the inner wall of the adjusting plate, the surface of the adjusting rod is rotatably connected to the inner wall of the rotating hole, the anti-slip pad is fixedly connected to the opposite side of the splint, the adjusting plate is slidably connected to the inner wall of the adjusting groove, the motor is fixedly connected to the left side of the adjusting rod, and the splint is fixedly connected to the front side of the adjusting plate.

[0010] In order to enable the snap-fit ​​assembly to achieve the effect of connecting the connecting tube on the purification device to the chromatography column, as a preferred antibody purification device of the present invention, the snap-fit ​​assembly includes a snap-fit ​​plate, a slide groove, a sleeve, a connecting tube and a connecting frame, the snap-fit ​​plate is fixedly connected to the bottom of the positioning plate, the rear side of the snap-fit ​​plate is fixedly connected to the front side of the main body, the slide groove is opened on the front side of the snap-fit ​​plate, the sleeve is fixedly connected to the inner wall of the connecting frame, the connecting tube is fixedly connected to the bottom of the sleeve, and the connecting frame is slidably connected to the front side of the snap-fit ​​plate.

[0011] In order to enable the injection component to achieve the effect of injecting the eluent into the chromatography column, as a preferred antibody purification device of the utility model, the injection component includes a sealed cover, a transfer tube, an air pump, a transparent injection tube and a support plate, the sealed cover is clamped on the top of the transparent injection tube, the transfer tube is fixedly connected to the rear side of the transparent injection tube, the air pump is fixedly connected to the left side of the support plate, the top of the air pump is fixedly connected to the bottom of the transfer tube, the transparent injection tube is fixedly connected to the front side of the support plate, the support plate is fixedly connected to the top of the positioning plate, and the rear side of the support plate is fixedly connected to the front side of the main body.

[0012] In order to enable the connecting plate to achieve the effect of connecting the chromatography column and the clamping plate, as a preferred antibody purification device of the utility model, the surface of the chromatography column is fixedly connected with the connecting plate, and the surface of the connecting plate is clamped with the inner wall of the clamping plate.

[0013] In order to enable the limiting block to achieve the effect of sliding connection with the slide groove, as a preferred antibody purification device of the present invention, the rear side of the connecting frame is fixedly connected with the limiting block, and the surface of the limiting block is slidably connected with the inner wall of the slide groove.

[0014] In order to enable the support plate to achieve the effect of connecting and supporting the electric telescopic rod, and to enable the electric telescopic rod to achieve the effect of lifting and lowering the connecting frame, as a preferred antibody purification device of the utility model, the bottom of the front side of the clamping plate is fixedly connected to a support block, the top of the support block is fixedly connected to the electric telescopic rod, and the top of the electric telescopic rod is fixedly connected to the bottom of the connecting frame.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] The antibody purification device is provided with a connection and positioning mechanism, wherein the connection and positioning mechanism includes a connection component and a positioning component. Through the provision of the connection component, the connection component can achieve the effect of connecting the positioning component, the snap-fit ​​component and the injection component, and at the same time, the connection component can also play the effect of limiting the positioning component. Through the provision of the positioning component, the positioning component is used in conjunction with the connection component, and the effect of positioning and fixing the chromatography column can be achieved, thereby facilitating the use of the snap-fit ​​component and the injection component. Through the provision of the snap-fit ​​component, the snap-fit ​​component can achieve the effect of connecting the connecting pipe on the purification device to the chromatography column. Through the provision of the injection component, the injection component can achieve the effect of injecting the eluent into the chromatography column, thereby making the connection and positioning mechanism cooperate with the snap-fit ​​component and the injection component, thereby solving the problem of manually connecting the pipeline on the purification device to the chromatography column, which is relatively troublesome, and solving the problem of manually using a dropper to draw the eluent and then dripping the eluent in the dropper into the chromatography column according to the required approximate amount, which is inconvenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the overall structural diagram of the antibody purification device of the utility model;

[0018] Figure 2 It is a three-dimensional schematic diagram of the connection positioning mechanism, the positioning component and the injection component in the utility model;

[0019] Figure 3 It is a three-dimensional schematic diagram of the connection assembly in the utility model;

[0020] Figure 4 It is a connection diagram of the positioning component in the utility model;

[0021] Figure 5 It is a connection diagram of the clamping assembly in the utility model;

[0022] Figure 6 This is a schematic diagram of the connection between the support block and the electric telescopic rod in the utility model;

[0023] Figure 7 It is a connection diagram of the injection assembly in the utility model.

[0024] In the figure, 1. connecting and positioning mechanism; 101. connecting component; 1011. positioning plate; 1012. clamping plate; 1013. adjusting slot; 1014. rotating hole; 102. positioning component; 1021. adjusting rod; 1022. anti-skid pad; 1023. adjusting plate; 1024. motor; 1025. clamping plate; 2. clamping component; 201. clamping plate; 202. slide slot; 203. sleeve; 204. connecting pipe; 205. connecting frame; 3. injection component; 301. sealing cover; 302. transmission pipe; 303. air pump; 304. transparent injection tube; 305. support plate; 4. chromatography column; 5. main body; 6. connecting plate; 7. limit block; 8. support block; 9. electric telescopic rod. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0026] In the description of the present invention, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, in the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0027] See also Figure 1-7 The utility model provides a technical solution: an antibody purification device, comprising a connection and positioning mechanism 1, a clamping assembly 2, an injection assembly 3, a chromatography column 4 and a main body 5, wherein the connection and positioning mechanism 1 is arranged at the front side of the main body 5, the clamping assembly 2 is arranged at the bottom of the connection and positioning mechanism 1, the injection assembly 3 is arranged at the top of the connection and positioning mechanism 1, and the chromatography column 4 is arranged at the front side of the connection and positioning mechanism 1;

[0028] The connection and positioning mechanism 1 comprises a connection component 101 and a positioning component 102 . The connection component 101 is arranged on the front side of the main body 5 , and the positioning component 102 is arranged on the front side of the connection component 101 .

[0029] In this embodiment: through the setting of the connection positioning mechanism 1, the connection positioning mechanism 1 includes a connection component 101 and a positioning component 102. Through the setting of the connection component 101, the connection component 101 can achieve the effect of connecting the positioning component 102, the clamping component 2 and the injection component 3. At the same time, the connection component 101 can also limit the positioning component 102. Through the setting of the positioning component 102, the positioning component 102 cooperates with the connection component 101 to achieve the effect of positioning and fixing the chromatography column 4, which is convenient for the use of the clamping component 2 and the injection component 3. Through the clamping component 2, the snap-fit ​​assembly 2 can achieve the effect of connecting the connecting tube 204 on the purification device to the chromatography column 4. Through the setting of the injection assembly 3, the injection assembly 3 can achieve the effect of injecting the eluent into the chromatography column 4, so that the connection positioning mechanism 1 is used in conjunction with the snap-fit ​​assembly 2 and the injection assembly 3, which solves the problem of manually connecting the pipeline on the purification device to the chromatography column 4, which is more troublesome, and manually using a dropper to draw the eluent and then dripping the eluent in the dropper into the chromatography column 4 according to the required approximate amount, and the inconvenience. The main body 5 is an existing device that can purify antibodies.

[0030] As a technical optimization solution of the utility model, the connecting component 101 includes a positioning plate 1011, a clamping plate 1012, an adjustment slot 1013 and a rotating hole 1014. The positioning plate 1011 is fixedly connected to the front side of the main body 5, the clamping plate 1012 is fixedly connected to the top of the front side of the positioning plate 1011, the adjustment slot 1013 is opened on the front side of the positioning plate 1011, and the rotating hole 1014 is opened on both sides of the positioning plate 1011.

[0031] In this embodiment: through the setting of the positioning plate 1011, the positioning plate 1011 can achieve the effect of connecting the card plate 1012, through the setting of the card plate 1012, the card plate 1012 can achieve the effect of clamping and positioning the connecting plate 6, through the setting of the adjustment groove 1013, the adjustment groove 1013 can achieve the effect of sliding connection with the adjustment plate 1023, and through the setting of the rotating hole 1014, the rotating hole 1014 can achieve the effect of rotationally connecting the adjustment rod 1021.

[0032] As a technical optimization solution of the utility model, the positioning assembly 102 includes an adjusting rod 1021, an anti-slip pad 1022, an adjusting plate 1023, a motor 1024 and a splint 1025. The adjusting rod 1021 is threadedly connected to the inner wall of the adjusting plate 1023, the surface of the adjusting rod 1021 is rotatably connected to the inner wall of the rotating hole 1014, the anti-slip pad 1022 is fixedly connected to the opposite side of the splint 1025, the adjusting plate 1023 is slidably connected to the inner wall of the adjusting groove 1013, the motor 1024 is fixedly connected to the left side of the adjusting rod 1021, and the splint 1025 is fixedly connected to the front side of the adjusting plate 1023.

[0033] In this embodiment: by setting the adjusting rod 1021, the adjusting rod 1021 is an existing bidirectional threaded rod, so that the effect of equidistant positioning adjustment of the adjusting plate 1023 can be achieved, and by setting the anti-skid pad 1022, the anti-skid pad 1022 can prevent sliding when clamping the chromatography column 4, and by setting the adjusting plate 1023, the adjusting plate 1023 can achieve the effect of connecting and fixing the splint 1025, and by setting the splint 1025, the splint 1025 can achieve the effect of clamping, positioning and fixing the chromatography column 4.

[0034] As a technical optimization solution of the utility model, the locking assembly 2 includes a locking plate 201, a slide groove 202, a sleeve 203, a connecting tube 204 and a connecting frame 205. The locking plate 201 is fixedly connected to the bottom of the positioning plate 1011, the rear side of the locking plate 201 is fixedly connected to the front side of the main body 5, the slide groove 202 is opened on the front side of the locking plate 201, the sleeve 203 is fixedly connected to the inner wall of the connecting frame 205, the connecting tube 204 is fixedly connected to the bottom of the sleeve 203, and the connecting frame 205 is slidably connected to the front side of the locking plate 201.

[0035] In this embodiment: by setting the snap plate 201, the snap plate 201 is used in conjunction with the slide groove 202 to achieve the effect of limiting the sliding connection of the limit block 7; by setting the sleeve 203, the sleeve 203 can achieve the effect of sleeve-connecting the chromatography column 4; by setting the connecting tube 204, the connecting tube 204 can achieve the effect of connecting the sleeve 203 and the main body 5; by setting the connecting frame 205, the connecting frame 205 can achieve the effect of connecting and fixing the sleeve 203.

[0036] As a technical optimization solution of the utility model, the injection assembly 3 includes a sealed cover 301, a transmission tube 302, an air pump 303, a transparent injection tube 304 and a support plate 305. The sealed cover 301 is clamped on the top of the transparent injection tube 304, the transmission tube 302 is fixedly connected to the rear side of the transparent injection tube 304, the air pump 303 is fixedly connected to the left side of the support plate 305, the top of the air pump 303 is fixedly connected to the bottom of the transmission tube 302, the transparent injection tube 304 is fixedly connected to the front side of the support plate 305, the support plate 305 is fixedly connected to the top of the positioning plate 1011, and the rear side of the support plate 305 is fixedly connected to the front side of the main body 5.

[0037] In this embodiment: by setting the sealing cover 301, the sealing cover 301 can achieve the effect of sealing the transparent injection tube 304, by setting the transmission tube 302, the transmission tube 302 can provide communication conditions for the air pump 303 and the transparent injection tube 304, by setting the air pump 303, the air pump 303 can inject gas into the transparent injection tube 304 through the transmission tube 302, and then increase the air pressure in the transparent injection tube 304, so that the eluent therein can be injected out, and a one-way valve is arranged inside the injection head of the transparent injection tube 304, and by setting the support plate 305, the support plate 305 can achieve the effect of connecting and supporting the transparent injection tube 304.

[0038] As a technical optimization solution of the present invention, a connection plate 6 is fixedly connected to the surface of the chromatography column 4 , and the surface of the connection plate 6 is clamped to the inner wall of the clamping plate 1012 .

[0039] In this embodiment, by providing the connecting plate 6 , the connecting plate 6 can achieve the effect of connecting the chromatography column 4 and the clamping plate 1012 .

[0040] As a technical optimization solution of the utility model, the rear side of the connecting frame 205 is fixedly connected to a limiting block 7 , and the surface of the limiting block 7 is slidably connected to the inner wall of the sliding groove 202 .

[0041] In this embodiment, by setting the limit block 7 , the limit block 7 can achieve the effect of sliding connection of the slide groove 202 .

[0042] As a technical optimization solution of the utility model, the bottom of the front side of the locking plate 201 is fixedly connected to a support block 8, the top of the support block 8 is fixedly connected to an electric telescopic rod 9, and the top of the electric telescopic rod 9 is fixedly connected to the bottom of the connecting frame 205.

[0043] In this embodiment: through the setting of the support block 8, the support block 8 can achieve the effect of connecting and supporting the electric telescopic rod 9, and through the setting of the electric telescopic rod 9, the electric telescopic rod 9 can achieve the effect of lifting and lowering the connecting frame 205.

[0044] Working principle: First, the chromatography column 4 loaded with antibodies after treatment is clamped into the positioning plate 1011 by using the connecting plate 6 to fix the chromatography column 4, and then the motor 1024 is turned on to drive the adjusting rod 1021 to rotate, and the adjusting plate 1023 is adjusted so that the clamping plate 1025 cooperates with the anti-slip pad 1022 to clamp and position the chromatography column 4, and then the electric telescopic rod 9 is turned on to adjust the connecting frame 205, so that the connecting frame 205 drives the sleeve 203 to be sleeved on the bottom of the chromatography column 4, so that the chromatography column 4 is connected with the connecting tube 204, and then connected with the purification device, and then the main body 5 can be used to purify the antibodies in the chromatography column 4, and then during the purification process, when eluent needs to be added, the air pump 303 is turned on to increase the air pressure in the transparent injection tube 304, so that the eluent in it is injected into the chromatography column 4.

[0045] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibody purification device, comprising a connection and positioning mechanism (1), a snap-fit ​​assembly (2), an injection assembly (3), a chromatography column (4) and a main body (5), characterized in that: The connection positioning mechanism (1) is arranged on the front side of the main body (5), the locking assembly (2) is arranged at the bottom of the connection positioning mechanism (1), the injection assembly (3) is arranged on the top of the connection positioning mechanism (1), and the chromatography column (4) is arranged on the front side of the connection positioning mechanism (1); The connection and positioning mechanism (1) comprises a connection component (101) and a positioning component (102); the connection component (101) is arranged on the front side of the main body (5); and the positioning component (102) is arranged on the front side of the connection component (101).

2. An antibody purification device according to claim 1, characterized in that: The connection assembly (101) comprises a positioning plate (1011), a clamping plate (1012), an adjustment slot (1013) and a rotation hole (1014); the positioning plate (1011) is fixedly connected to the front side of the main body (5); the clamping plate (1012) is fixedly connected to the top of the front side of the positioning plate (1011); the adjustment slot (1013) is provided on the front side of the positioning plate (1011); and the rotation hole (1014) is provided on both sides of the positioning plate (1011).

3. An antibody purification device according to claim 1, characterized in that: The positioning assembly (102) comprises an adjusting rod (1021), an anti-skid pad (1022), an adjusting plate (1023), a motor (1024) and a clamping plate (1025); the adjusting rod (1021) is threadedly connected to the inner wall of the adjusting plate (1023); the surface of the adjusting rod (1021) is rotatably connected to the inner wall of the rotating hole (1014); the anti-skid pad (1022) is fixedly connected to the opposite side of the clamping plate (1025); the adjusting plate (1023) is slidably connected to the inner wall of the adjusting groove (1013); the motor (1024) is fixedly connected to the left side of the adjusting rod (1021); and the clamping plate (1025) is fixedly connected to the front side of the adjusting plate (1023).

4. An antibody purification device according to claim 1, characterized in that: The engaging assembly (2) comprises an engaging plate (201), a slide groove (202), a sleeve (203), a connecting tube (204) and a connecting frame (205); the engaging plate (201) is fixedly connected to the bottom of the positioning plate (1011); the rear side of the engaging plate (201) is fixedly connected to the front side of the main body (5); the slide groove (202) is provided on the front side of the engaging plate (201); the sleeve (203) is fixedly connected to the inner wall of the connecting frame (205); the connecting tube (204) is fixedly connected to the bottom of the sleeve (203); and the connecting frame (205) is slidably connected to the front side of the engaging plate (201).

5. The antibody purification device according to claim 1, characterized in that: The injection assembly (3) comprises a sealed cover (301), a transmission tube (302), an air pump (303), a transparent injection tube (304) and a support plate (305); the sealed cover (301) is clamped on the top of the transparent injection tube (304); the transmission tube (302) is fixedly connected to the rear side of the transparent injection tube (304); the air pump (303) is fixedly connected to the left side of the support plate (305); the top of the air pump (303) is fixedly connected to the bottom of the transmission tube (302); the transparent injection tube (304) is fixedly connected to the front side of the support plate (305); the support plate (305) is fixedly connected to the top of the positioning plate (1011); and the rear side of the support plate (305) is fixedly connected to the front side of the main body (5).

6. An antibody purification device according to claim 2, characterized in that: A connecting plate (6) is fixedly connected to the surface of the chromatography column (4), and the surface of the connecting plate (6) is clamped to the inner wall of the clamping plate (1012).

7. An antibody purification device according to claim 4, characterized in that: The rear side of the connection frame (205) is fixedly connected to a limiting block (7), and the surface of the limiting block (7) is slidably connected to the inner wall of the sliding groove (202).

8. An antibody purification device according to claim 4, characterized in that: The bottom of the front side of the clamping plate (201) is fixedly connected to a support block (8), the top of the support block (8) is fixedly connected to an electric telescopic rod (9), and the top of the electric telescopic rod (9) is fixedly connected to the bottom of the connecting frame (205).

Images