Cell culture bottle bracket

Abstract

The utility model discloses a cell culture bottle support which comprises a guide frame and a transverse pushing mechanism, the guide frame comprises two parallel guide rods, the two guide rods are respectively provided with a protruding rod portion in an upward protruding mode, the protruding rod portions are respectively provided with a staggered rod portion in an upward protruding mode, the two staggered rod portions are sequentially arranged in the axial direction of the guide rods, and the transverse pushing mechanism is arranged between the two staggered rod portions. The convex rod part and the guide rod are in smooth transition through an inclined rod, and the convex rod part and the staggered rod part are in smooth transition through an inclined rod; the transverse pushing mechanism is arranged at the bottom of the guide frame, and the movable end of the transverse pushing mechanism upwards penetrates through a gap between the two guide rods and is used for pushing the cell culture bottle to move along the guide frame. According to the utility model, through the convex rod part and the staggered rod part on the guide rod, when the transverse pushing mechanism pushes the cell culture bottle along the length direction of the guide rod, inclination from two sides and front and back is formed, so that an automatic shaking effect is achieved, stem cells are in full contact with digestive enzymes, manual shaking is omitted, and the operation efficiency in the cell culture process is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture tools, in particular to a cell culture bottle bracket. Background Art

[0002] At present, in cell culture, cell culture bottles are often used for cell culture. For example, Chinese patent 201320527048.9 discloses a cell culture bottle, wherein the card edge on the front panel of the cell culture bottle can be inserted into the card groove and the flange on the rear panel of another cell culture bottle; the joint of the cell culture bottle includes two triangular plates and a trapezoidal plate, and the angle between the trapezoidal plate and the front panel is 130°; the lower side panel, the bottom panel, and the upper side panel are inclined toward the inner side of the bottle body, and the bottleneck is inclined toward the rear panel, and a certain inclination angle is set.

[0003] For the above-mentioned prior art, in the subculture of stem cells, it is necessary to inject digestive enzymes into the culture bottles, and the stem cells in the culture bottles are fully in contact with the digestive enzymes to ensure that most of the cells are suspended and rounded. This process requires staff to shake the cell culture bottles one by one to allow the digestive enzymes to fully contact the stem cells. Manual shaking is inefficient and takes up the time for the next cell culture bottle to be filled. Therefore, in order to improve the operational efficiency of subculture, how to automatically shake the cell culture bottles is a technical problem that needs to be solved. Utility Model Content

[0004] The purpose of the utility model is to overcome the above technical deficiencies, and to provide a cell culture bottle support to solve the technical problem of how to automatically shake the cell culture bottle in the prior art.

[0005] In order to achieve the above technical purpose, the utility model adopts the following technical solutions:

[0006] The utility model provides a cell culture bottle bracket, comprising:

[0007] A guide frame, comprising two parallel guide rods, each of the two guide rods having a protruding rod portion protruding upward, and each of the protruding rod portions having a staggered rod portion protruding upward, the two staggered rod portions being sequentially arranged along the axial direction of the guide rod, and a slanted rod being used for smooth transition between the protruding rod portion and the guide rod, and between the protruding rod portion and the staggered rod portion; and

[0008] The horizontal pushing mechanism is arranged at the bottom of the guide frame, and its movable end passes upward from the gap between the two guide rods, and is used for pushing the cell culture bottle to move along the guide frame.

[0009] In some embodiments, both ends of the two guide rods are straight to form a storage platform for placing cell culture bottles.

[0010] In some embodiments, a baffle is also included, which is arranged on the front and rear sides of the transverse pushing mechanism and is positioned in front of and behind the guide frame.

[0011] In some embodiments, a lifting mechanism is also included, wherein a movable end of the lifting mechanism is connected to the transverse pushing mechanism to drive the transverse pushing mechanism and the guide frame to rise and fall together.

[0012] In some embodiments, the horizontal pushing mechanism includes a base, a first wire lever, a push plate and a first drive motor. The first wire lever is rotatably connected to the inner side of the base, the first drive motor is arranged at one end of the base, and its output shaft is connected to the end of the first wire lever, and the push plate is slidably connected to the inner side of the base and is threadedly connected to the first wire lever.

[0013] In some embodiments, two support rods are disposed at the bottom of the guide rod, and the bottom ends of the support rods are connected to the base.

[0014] In some embodiments, the lifting mechanism includes a guide seat, a second wire lever, a lifting block and a second drive motor, the lifting block is connected to the base, the second wire lever is rotatably connected to the inner side of the guide seat, the second drive motor is arranged at the top end of the guide seat, and its output shaft is connected to the end of the second wire lever, the lifting block is slidably connected in the guide seat, and is threadedly connected to the second wire lever.

[0015] In some embodiments, the push plate includes a horizontal push plate body and an extension plate, and the extension plates are respectively arranged on both sides of the horizontal push plate body, and the thickness of the extension plate on one side is greater than the sum of the base wall thickness and the length of the first drive motor, and the thickness of the extension plate on the other side is greater than the base wall thickness.

[0016] In some embodiments, the length of the guide frame is equal to the length of the base.

[0017] In some embodiments, proximity sensors are disposed on inner walls at both ends of the base.

[0018] Compared with the prior art, the cell culture bottle holder provided by the utility model, through the raised rod portion and the staggered rod portion on the guide rod, forms an inclination on both sides and front and back when the horizontal pushing mechanism pushes the cell culture bottle along the length direction of the guide rod, thereby achieving an automatic shaking effect, allowing the stem cells to fully contact with the digestive enzymes, eliminating manual shaking and improving the operating efficiency during the cell culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a three-dimensional diagram of a cell culture bottle support provided by an embodiment of the utility model;

[0020] Figure 2 It is a three-dimensional diagram of the guide frame and the horizontal thrust mechanism provided by the embodiment of the utility model;

[0021] Figure 3 It is a front view of the guide frame and the horizontal thrust mechanism provided by the embodiment of the utility model;

[0022] Figure 4 It is a cross-sectional view of the horizontal thrust mechanism provided by the embodiment of the utility model.

[0023] Description of reference numerals:

[0024] 1. Guide frame; 11. Guide rod; 12. Raised rod; 13. Staggered rod; 101. Storage platform; 102. Support rod;

[0025] 2. Horizontal push mechanism; 21. Base; 22. First wire lever; 23. Push plate; 24. First drive motor; 231. Horizontal push plate; 232. Extension plate;

[0026] 3. Baffle;

[0027] 4. Lifting mechanism; 41. Guide seat; 42. Second wire lever; 43. Lifting block; 44. Second driving motor;

[0028] 5. Cell culture flask. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0030] In order to solve the technical problem of how to automatically shake a cell culture bottle, the utility model provides a cell culture bottle bracket, which can realize automatic shaking of the cell culture bottle.

[0031] It should be noted that the cell culture flask holder described in the present invention is used for but not limited to stem cell culture, etc. For the convenience of explanation, in the present invention, only the application of the cell culture flask holder to stem cell culture is used as an example for explanation, and the principle of applying the cell culture flask holder to other types of equipment is essentially the same as the principle applied to stem cell culture, which will not be repeated here.

[0032] See also Figure 1 , Figure 1The schematic diagram of the structure of the cell culture bottle support in one embodiment of the utility model is shown. The cell culture bottle support includes a guide frame 1 and a horizontal pushing mechanism 2. The guide frame 1 includes two parallel guide rods 11. The two guide rods 11 are both provided with a raised rod portion 12 protruding upwards, and the raised rod portion 12 is provided with a staggered rod portion 13 protruding upwards. The staggered rod portions 13 on the two guide rods 11 are staggered in front and back distribution. The raised rod portion 12 and the guide rod 11, and the raised rod portion 12 and the staggered rod portion 13 are smoothly transitioned by oblique rods. The cell culture bottle 5 is placed on the guide rod 11. When one end of the guide rod 11 slides along the length direction of the guide rod 11, it first moves from the end of the guide rod 11 to the protruding rod portion 12 to form a side tilt, and shakes the cell culture bottle 5 sideways, and then moves from the protruding rod portion 12 to the staggered rod portion 13, and then shakes the cell culture bottle 5 forward and backward in turn, and then moves from the protruding rod portion 12 to the other end of the guide rod 11, and then shakes the other side sideways, so that in the process of moving along the entire guide frame 1, tilt shaking is formed on both sides and front and back, which can enable the stem cells to fully contact with the digestive enzymes.

[0033] In this embodiment, the horizontal pushing mechanism 2 is arranged at the bottom of the guide frame 1, and its movable end passes upward from the gap between the two guide rods 11, and is used to push the cell culture bottle to move along the guide frame 1, thereby automatically pushing the cell culture bottle 5 to move along the guide frame 1, forming an inclined shaking on both sides and front and back, so that the stem cells are fully in contact with the digestive enzymes.

[0034] It can be understood that, in use, the cell culture bottle 5 only needs to be placed at one end of the two guide rods 11 and moved by the mechanical energy of the horizontal pushing mechanism 2. During this process, the operator can perform liquid injection operation on the next cell culture bottle 5 to improve the operational efficiency of the entire stem cell subculture.

[0035] In one embodiment, see Figure 1 , Figure 2 and Figure 3 In order to place the cell culture bottles 5 at both ends to form a reciprocating movement of the cell culture bottles 5 and improve the processing efficiency, both ends of the two guide rods 11 are straight to form a placement platform 101 for placing the cell culture bottles.

[0036] In this embodiment, the cell culture bottle 5 is first placed at one end, and after the cell culture bottle 5 is pushed to the other end, the cell culture bottle 5 is placed with the other end as the starting point, and moved back to the initial position in the opposite direction, forming a reciprocating pushing of the cell culture bottle 5, avoiding waiting time for the return push.

[0037] In one embodiment, see Figure 1In order to prevent the cell culture bottle 5 from sliding off due to excessive forward and backward inclination of the cell culture bottle 5, the cell culture bottle holder also includes a baffle 3, which is arranged at the front and rear sides of the horizontal pushing mechanism 2 and is positioned in front of and behind the guide frame 1. It is mainly used to shield the front and rear ends of the cell culture bottle 5 to prevent it from sliding off the guide frame 1.

[0038] In this embodiment, the height of the baffle 3 is based on the maximum height of the cell culture bottle 5 on the staggered rod 13 to prevent it from falling off, and the distance between the front and rear baffles 3 is a distance that does not affect the forward and backward tilting of the cell culture bottle 5.

[0039] In one embodiment, see Figure 1 In order to stack the cell culture bottles 5 after shaking and facilitate the subsequent transfer by operators, the cell culture bottle holder also includes a lifting mechanism 4, the movable end of the lifting mechanism 4 is connected to the horizontal pushing mechanism 2, and is used to drive the horizontal pushing mechanism 2 and the guide frame 1 to rise and fall together. After pushing out a cell culture bottle 5, the lifting mechanism 4 raises the height of a cell culture bottle 5, so that when the cell culture bottle 5 is pushed out next time, it can be directly stacked on the top of the previous cell culture bottle 5, thereby forming a stacking effect.

[0040] Furthermore, in order to achieve a horizontal pushing effect on the cell culture bottle 5, the horizontal pushing mechanism 2 includes a base 21, a first wire lever 22, a push plate 23 and a first drive motor 24. The first wire lever 22 is rotatably connected to the inner side of the base 21. The first drive motor 24 is arranged at one end of the base 21, and its output shaft is connected to the end of the first wire lever 22. The push plate 23 is slidably connected to the inner side of the base 21 and is threadedly connected to the first wire lever 22. The first wire lever 22 is driven to rotate by the first drive motor 24, prompting the push plate 23 to slide along the base 21, thereby pushing the cell culture bottle 5.

[0041] Furthermore, in order to have a certain height difference between the guide rod 11 and the horizontal pushing mechanism 2 , two support rods 102 are provided at the bottom of the guide rod 11 , and the bottom ends of the support rods 102 are connected to the base 21 .

[0042] Furthermore, in order to lift and lower the transverse pushing mechanism 2 and the guide frame 1, the lifting mechanism 4 includes a guide seat 41, a second wire lever 42, a lifting block 43 and a second drive motor 44. The lifting block 43 is connected to the base 21. The second wire lever 42 is rotatably connected to the inner side of the guide seat 41. The second drive motor 44 is arranged at the top end of the guide seat 41, and its output shaft is connected to the end of the second wire lever 42. The lifting block 43 is slidably connected in the guide seat 41 and is threadedly connected to the second wire lever 42. The second wire lever 42 is driven to rotate by the second drive motor 44, prompting the lifting block 43 to slide along the guide seat 41, thereby driving the transverse pushing mechanism 2 and the guide frame 1 to be lifted and lowered.

[0043] It can be understood that both the second drive motor 44 and the first drive motor 24 can be stepper motors.

[0044] In one embodiment, see Figure 4 In order to completely push the cell culture bottle 5 out of the horizontal pushing mechanism 2, the pushing plate 23 includes a horizontal pushing plate body 231 and an extension plate 232. The extension plates 232 are respectively arranged on both sides of the horizontal pushing plate body 231, and the thickness of the extension plate 232 on one side is greater than the sum of the wall thickness of the base 21 and the length of the first driving motor 24, so that the cell culture bottle 5 can be pushed out of the horizontal pushing mechanism 2, and the thickness of the extension plate 232 on the other side is greater than the wall thickness of the base 21, so that the cell culture bottle 5 can be pushed out of the horizontal pushing mechanism 2, so as to ensure that the cell culture bottle 5 can be pushed out of the horizontal pushing mechanism 2 at both ends and spread flat on the desktop or stacked on the previous cell culture bottle 5.

[0045] Furthermore, in order to prevent the guide frame 1 from interfering with the stacking, the length of the guide frame 1 is equal to the length of the base 21 , so that when it is pushed outside the horizontal pushing mechanism 2 , it is synchronously pushed outside the guide frame 1 .

[0046] In one of the embodiments, in order to form an automatic stop in position, proximity sensors are provided on the inner walls at both ends of the base 21, and position feedback is formed by the proximity sensors. When the push plate 23 reaches both ends, it is detected by the proximity sensors and feedback is formed, thereby prompting the first drive motor 24 to stop driving and prepare for reverse rotation drive.

[0047] It can be understood that in order to make it easier for operators to use, a controller can also be provided. Correspondingly, a touch-pressure switch is provided on the extension plate 232. When the cell culture bottle 5 is placed on the storage platform 101 and the touch-pressure switch on the corresponding side extension plate 232 is pressed, feedback is given to the controller, and the controller controls the first drive motor 24 to drive the cell culture bottle 5 to move. When it moves to the other end, it is detected by the proximity sensor and feedback is formed. The controller controls the first drive motor 24 to stop driving and prepare for reverse rotation. According to the above principle, the cell culture bottle 5 is placed on this end again, and the touch-pressure switch on the corresponding side extension plate 232 is pressed to push the cell culture bottle 5 in the opposite direction. This reciprocating process is repeated to simplify the operation.

[0048] It should be noted that the above control means are conventional technical means in the art and will not be elaborated in detail here.

[0049] In order to better understand the present invention, the following Figures 1 to 4 The technical solution of the utility model is described in detail: after the operator injects the liquid into the cell culture bottle 5, the cell culture bottle 5 is placed flat on the two parallel guide rods 11 of the guide frame 1, specifically placed on the position of the storage platform 101 at the starting end, and the push plate 23 is attached to one side of the cell culture bottle 5. When the horizontal push mechanism 2 is started, the cell culture bottle 5 is automatically pushed from one end to the other end along the length direction of the guide rod 11. During the pushing process, it will go through ups and downs, specifically the raised rod part 12 and the staggered rod part 13, forming an inclination on both sides and front and back, so as to achieve a shaking effect, which can make the stem cells and the digestive tract The enzymes are fully in contact, so that the operator can perform the liquid injection operation of the next cell culture bottle 5 during this time interval; since the push plate 23 moves to the position of the storage platform 101 at the other end, after the liquid injection operation, the next cell culture bottle 5 can be placed on the storage platform 101 at this end, and pushed in the opposite direction to the starting end, and the stem cells in the cell culture bottle 5 are fully in contact with the digestive enzymes; and after the cell culture bottle 5 is pushed once at both ends, the lifting mechanism 4 is lifted by one layer, and the height of one layer is the height of the cell culture bottle 5 lying flat, so that the cell culture bottles 5 are stacked.

[0050] The specific implementation methods of the utility model described above do not constitute a limitation on the protection scope of the utility model. Any other corresponding changes and modifications made according to the technical concept of the utility model should be included in the protection scope of the claims of the utility model.

Claims

1. A cell culture flask support, characterized in that: include: The guide frame comprises two parallel guide rods, each of the two guide rods has a protruding rod portion protruding upward, and each of the protruding rod portions has a staggered rod portion protruding upward, the two staggered rod portions are sequentially arranged along the axial direction of the guide rod, and the protruding rod portion and the guide rod, and the protruding rod portion and the staggered rod portion are smoothly transitioned by oblique rods; as well as The horizontal pushing mechanism is arranged at the bottom of the guide frame, and its movable end passes upward from the gap between the two guide rods, and is used for pushing the cell culture bottle to move along the guide frame.

2. The cell culture flask support according to claim 1, characterized in that: Both ends of the two guide rods are straight, forming a placement platform for placing cell culture bottles.

3. The cell culture flask support according to claim 2, characterized in that: It also includes baffles, which are arranged on the front and rear sides of the transverse pushing mechanism and are positioned in front of and behind the guide frame.

4. The cell culture flask support according to claim 3, characterized in that: It also includes a lifting mechanism, the movable end of which is connected to the transverse pushing mechanism and is used to drive the transverse pushing mechanism and the guide frame to rise and fall together.

5. The cell culture flask support according to claim 4, characterized in that: The horizontal pushing mechanism includes a base, a first wire lever, a push plate and a first driving motor. The first wire lever is rotatably connected to the inner side of the base. The first driving motor is arranged at one end of the base, and its output shaft is connected to the end of the first wire lever. The push plate is slidably connected to the inner side of the base and is threadedly connected to the first wire lever.

6. The cell culture flask support according to claim 5, characterized in that: Two support rods are arranged at the bottom of the guide rod, and the bottom ends of the support rods are connected to the base.

7. The cell culture flask support according to claim 6, characterized in that: The lifting mechanism includes a guide seat, a second wire lever, a lifting block and a second drive motor. The lifting block is connected to the base. The second wire lever is rotatably connected to the inner side of the guide seat. The second drive motor is arranged at the top end of the guide seat, and its output shaft is connected to the end of the second wire lever. The lifting block is slidably connected in the guide seat and is threadedly connected to the second wire lever.

8. The cell culture flask support according to claim 7, characterized in that: The push plate includes a horizontal push plate body and an extension plate, wherein the extension plates are respectively arranged on both sides of the horizontal push plate body, and the thickness of the extension plate on one side is greater than the sum of the base wall thickness and the length of the first drive motor, and the thickness of the extension plate on the other side is greater than the base wall thickness.

9. The cell culture flask support according to claim 8, characterized in that: The length of the guide frame is equal to the length of the base.

10. The cell culture flask support according to claim 9, characterized in that: The inner walls at both ends of the base are provided with proximity sensors.

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