Cooling device for stem cell cryopreservation

Abstract

The utility model discloses a cooling device for stem cell cryopreservation, and relates to the field of stem cell cryopreservation, the upper end of a cooling box is provided with a top cover, the bottom end of the top cover is fixedly connected with an adjusting plate, the adjusting plate is clamped and connected in the cooling box, the top end of the cooling box is provided with a plurality of placing grooves, and the placing grooves are arranged in the cooling box. The placing grooves are communicated through liquid nitrogen filling holes, and gas storage grooves are formed in the positions, on the two sides of the placing grooves, of the interior of the cooling box. According to the cooling device for stem cell cryopreservation, by means of the weight of a cell cryopreservation tank, a movable block is pressed downwards, so that air in an air bag is extruded out and conveyed into an air storage groove through a second hose, a piston rod is pushed upwards by the air, and the piston rod drives a rotating rod to conduct height adjustment; and therefore, the bottom end of the rotating rod and the top end of the movable block are both attached to the cell cryopreservation tank, the cell cryopreservation tank can be stably placed, and shaking and deviation of the cell cryopreservation tank in the position are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell cryopreservation, in particular to a cooling device for stem cell cryopreservation. Background Art

[0002] Stem cells are a type of cell in the body with multidirectional differentiation potential. When storing stem cells, they should meet two conditions: constant low temperature and 80% humidity. At the same time, stem cells should be stored in a dedicated safety container to ensure the integrity and purity of the stem cells. Therefore, when freezing stem cells, a cooling device is needed to assist in cooling the cell freezing tank to facilitate subsequent cell preservation.

[0003] In the prior art, the Chinese patent with authorization announcement number CN214113569U discloses a cooling device for cryopreservation of stem cells with good sealing performance, including a box, an insulation layer, a freezing cylinder, a storage tube, a delivery tube, a liquid inlet tube, a valve, a pressure relief tube and a cover plate, a sealing groove is provided on the upper part of the inner wall of the box, and a sealing plate is movably installed in the sealing groove, and the sealing plate is arranged through the box, the bottom surface of the insulation layer is fixedly installed on the inner wall of the box, and the inner wall of the insulation layer is arranged in affixed with the outer wall of the freezing cylinder, and the lower end of the freezing cylinder is fixedly installed on the inner wall of the box, and the end of the delivery tube is fixedly installed on the freezing cylinder, and the delivery tube is arranged through the outer wall of the freezing cylinder. The cooling device for cryopreservation of stem cells with good sealing performance is small in size, convenient for rapid transportation of stem cells, and the sealing and heat preservation effect of the device is better, which can effectively slow down the heating speed, prevent the inactivation of stem cells, and the stem cell storage tube can be quickly taken.

[0004] The above-mentioned mechanism achieves a sealing effect by utilizing a sealing groove and a sealing plate, thereby increasing the internal heat preservation effect. However, in actual use, the sealing groove and the sealing plate need to be pushed in with force when sealing and engaging, and the sealing plate also needs to be pulled out with effort, which makes the cooling device inconvenient to open or close, affecting the use of the cooling device. Utility Model Content

[0005] The utility model aims to provide a cooling device for cryopreservation of stem cells, so as to solve the problem in the background art that the cooling device body needs to be repeatedly pulled out with force during the sealing process, causing inconvenience in the use of the cooling device body.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cooling device for cryopreservation of stem cells, wherein a top cover is provided at the upper end of a cooling box, and an adjustment plate is fixedly connected to the bottom end of the top cover, and the adjustment plate is snap-connected to the inside of the cooling box, a plurality of placement slots are provided at the top end of the cooling box, and the placement slots are connected through liquid nitrogen filling holes, and connecting pipes are fixedly connected to both ends of the cooling box;

[0007] The cooling boxes on both sides of the placement tank are provided with gas storage tanks, and the gas storage tanks are provided with limiting mechanisms inside, and the limiting mechanisms are used to limit the position of the cell freezing tanks, so that the cell freezing tanks can be placed more stably;

[0008] A sealing layer is provided at the upper end of the inner side of the cooling box, and sealing mechanisms are provided on both sides of the outer side of the cooling box. The sealing mechanisms are used to inflate the sealing layer to achieve sealing, thereby preventing the loss of liquid nitrogen temperature and playing a role of sealing and heat preservation.

[0009] Furthermore, the sealing mechanism includes an air storage plate, and the air storage plate is fixedly connected to one end of both sides of the cooling box, the inside of the air storage plate is slidably connected with a movable plate, one end of both sides of the bottom end of the top cover is fixedly connected with a pressing plate, and the pressing plate is arranged above the movable plate, and the lower end of one side of the air storage plate is fixedly connected with a first hose, and the first hose is connected to the sealing layer.

[0010] Furthermore, locking mechanisms are provided at both ends of the pressing plate and the movable plate, and the locking mechanisms are used to limit the position between the movable plate and the pressing plate to prevent the movable plate and the pressing plate from separating, thereby playing a role of stable sealing. The locking mechanism includes a first connecting plate, and the first connecting plate is fixedly connected to the two ends of the pressing plate, and the upper ends of the two ends of the movable plate are fixedly connected to the second connecting plates, and the positions of the first connecting plate and the second connecting plates correspond to each other, and a limiting rod is fixedly connected at the middle position of the bottom end of the first connecting plate, and the limiting rod passes through and extends to the bottom of the second connecting plate.

[0011] Furthermore, a fixing groove is provided on one side of the limiting rod, and the interior of the fixing groove is fixedly connected to the limiting block via a connecting spring, and one side of the limiting block passes through and extends to the outside of the limiting rod.

[0012] Furthermore, the fixing groove and the connecting spring are connected, and the connecting spring and the limiting block are welded into an integrated structure.

[0013] Furthermore, the limiting mechanism includes an airbag, and the airbag is arranged inside the placement groove, a movable block is fixedly connected to the top of the airbag, and the movable block and the placement groove are slidably connected, a second hose is fixedly connected to one side of the airbag, and one side of the second hose passes through and extends to the inside of the air storage tank, a piston rod is slidably connected to the inside of the air storage tank, and a rotating rod is hinged at the top of the piston rod.

[0014] Furthermore, the rotating rod is arranged above the placement slot, and the rotating rod is symmetrical about the central axis of the placement slot in the vertical direction.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. Flip the rotating rod and place the cell cryopreservation can inside the placement tank, flip the rotating rod to place it above the placement tank, use the weight of the cell cryopreservation can itself to press the movable block downward, so that the gas inside the airbag is squeezed out and transported to the inside of the gas storage tank through the second hose, and the gas pushes the piston rod upward, so that the piston rod drives the rotating rod to adjust the height, so that the bottom end of the rotating rod and the top end of the movable block are both fitted between the cell cryopreservation can, so that the cell cryopreservation can can be stably placed to avoid shaking and displacement of the cell cryopreservation can;

[0017] Furthermore, the rotating rod can be flexibly flipped, so that the flipping angle can be adjusted according to different states, so as to facilitate the positioning of the cell freezing tank or to facilitate the removal and placement. At the same time, the movable block can be used to flexibly adjust the different heights of the piston rod, so as to facilitate the adjustment according to the cell freezing tanks of different heights.

[0018] Furthermore, liquid nitrogen can be transported through the connecting pipe, and can be filled into each placement tank through the liquid nitrogen filling hole, thereby effectively playing the role of cell freezing. At the same time, the liquid nitrogen filling hole can be used to evenly fill the liquid nitrogen, avoiding the situation of incomplete freezing caused by uneven liquid nitrogen. At the same time, excess liquid nitrogen is discharged from the interior of the cooling box through another connecting pipe, avoiding the situation of safety accidents caused by liquid nitrogen leakage;

[0019] 2. The bottom end of the pressing plate contacts the movable plate, and the weight of the top cover and the adjusting plate is used to press downward, so that the movable plate squeezes the gas inside the gas storage plate and transports it to the inside of the sealing layer through the first hose. The sealing layer can fill the gap between the adjusting plate and the cooling box, thereby playing a sealing role and effectively preventing the temperature loss of liquid nitrogen;

[0020] Furthermore, after the sealing layer is sealed, the positions of the first connecting plate and the second connecting plate are aligned, the limiting rod is passed through the second connecting plate, and the limiting block pops out from the inside of the fixed groove by the elastic force of the connecting spring. At the same time, the limiting block limits the positions of the second connecting plate and the first connecting plate. Since the height to which the movable plate can move inside the gas storage plate is limited, after limiting the pressing plate and the movable plate, the separation between the movable plate and the pressing plate due to arbitrary pulling out can be avoided, thereby making the sealing of the sealing layer more secure. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the front view and cross-section of the three-dimensional structure of the utility model;

[0022] Figure 2 It is a side view cross-sectional three-dimensional structure schematic diagram of the fixing plate of the utility model;

[0023] Figure 3This is a schematic diagram of the three-dimensional structure of the utility model;

[0024] Figure 4 It is a schematic diagram of the side-view cutaway three-dimensional structure of the utility model;

[0025] Figure 5 It is a partially enlarged schematic diagram of a novel structure of the practical limit mechanism;

[0026] Figure 6 This is a partially enlarged schematic diagram of a novel structure of the practical sealing mechanism;

[0027] Figure 7 It is a schematic diagram of a side cross-sectional three-dimensional structure of a limit rod of the utility model.

[0028] In the figure: 1. cooling box; 2. top cover; 3. adjusting plate; 4. sealing layer; 5. placement groove; 6. liquid nitrogen filling hole; 7. connecting pipe; 8. gas storage plate; 9. movable plate; 10. pressing plate; 11. first hose; 12. first connecting plate; 13. second connecting plate; 14. limiting rod; 15. fixing groove; 16. connecting spring; 17. limiting block; 18. movable block; 19. airbag; 20. second hose; 21. gas storage tank; 22. piston rod; 23. rotating rod. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] Embodiment 1:

[0031] like Figure 1 , Figure 4 and Figure 5 The technical solution shown is to solve the problem that the cell freezing tank is easy to shake and cannot be fixed after being placed: the cooling device for stem cell freezing discloses a limiting mechanism, a top cover 2 is provided at the upper end of the cooling box 1, and an adjustment plate 3 is fixedly connected to the bottom end of the top cover 2, and the adjustment plate 3 is snap-connected to the inside of the cooling box 1, a plurality of placement grooves 5 are provided at the top of the cooling box 1, and the placement grooves 5 are connected through liquid nitrogen filling holes 6, and both ends of the cooling box 1 are fixedly connected with connecting pipes 7;

[0032] The cooling boxes 1 on both sides of the placement slot 5 are provided with gas storage slots 21, and the gas storage slots 21 are provided with limiting mechanisms inside. The limiting mechanisms are used to limit the position of the cell freezing tank, so that the cell freezing tank can be placed more stably.

[0033] A sealing layer 4 is provided at the upper end of the inner side of the cooling box 1, and sealing mechanisms are provided on both sides of the outer side of the cooling box 1. The sealing mechanisms are used to inflate the sealing layer 4 to achieve sealing, thereby preventing the loss of liquid nitrogen temperature and playing a role of sealing and heat preservation.

[0034] The limiting mechanism includes an airbag 19, and the airbag 19 is arranged inside the placement groove 5. A movable block 18 is fixedly connected to the top of the airbag 19, and the movable block 18 and the placement groove 5 are slidably connected. A second hose 20 is fixedly connected to one side of the airbag 19, and one side of the second hose 20 passes through and extends to the inside of the air storage tank 21. A piston rod 22 is slidably connected to the inside of the air storage tank 21, and a rotating rod 23 is hinged at the top of the piston rod 22.

[0035] The rotating rod 23 is disposed above the placement slot 5 , and the rotating rod 23 is symmetrical about the central axis of the placement slot 5 in the vertical direction.

[0036] In this example, the rotating rod 23 is flipped over and the cell freezing jar is placed inside the placement groove 5. The rotating rod 23 is flipped over to be placed above the placement groove 5. The movable block 18 is pressed downward by utilizing the weight of the cell freezing jar itself, so that the gas inside the airbag 19 is squeezed out and transported to the inside of the gas storage tank 21 through the second hose 20. The gas pushes the piston rod 22 upward, so that the piston rod 22 drives the rotating rod 23 to adjust the height, so that the bottom end of the rotating rod 23 and the top end of the movable block 18 are both in contact with the cell freezing jar, so that the cell freezing jar can be stably placed to avoid shaking and displacement of the position of the cell freezing jar.

[0037] Embodiment 2:

[0038] like Figure 2 , Figure 3 and Figure 6 The technical solution shown is to solve the problem that the connection between the cooling box 1 and the top cover 2 after installation is prone to gaps that lead to temperature loss: the cooling device for cryopreservation of stem cells discloses a sealing mechanism, which includes an air storage plate 8, and the air storage plate 8 is fixedly connected to one end of both sides of the cooling box 1, and the inside of the air storage plate 8 is slidably connected to a movable plate 9, one end of both sides of the bottom end of the top cover 2 is fixedly connected to a pressing plate 10, and the pressing plate 10 is arranged above the movable plate 9, and the lower end of one side of the air storage plate 8 is fixedly connected to a first hose 11, and the first hose 11 is connected to the sealing layer 4.

[0039] In this example, the top cover 2 is placed above the cooling box 1 so that the adjusting plate 3 at the bottom end of the top cover 2 can be inserted into the cooling box 1. At the same time, the bottom end of the pressing plate 10 is in contact with the movable plate 9. The weight of the top cover 2 and the adjusting plate 3 is used to press downward so that the movable plate 9 squeezes the gas inside the gas storage plate 8 and transports it to the inside of the sealing layer 4 through the first hose 11. The sealing layer 4 can fill the gap between the adjusting plate 3 and the cooling box 1, thereby playing a sealing role and effectively preventing the temperature loss of liquid nitrogen.

[0040] Embodiment three:

[0041] like Figure 2 , Figure 3 , Figure 6 and Figure 7 The technical solution shown is based on Example 2, and in order to solve the problem of loose sealing caused by gas leakage when the sealing mechanism is in use: the cooling device for cryopreservation of stem cells discloses a locking mechanism, and locking mechanisms are provided at both ends of the pressing plate 10 and the movable plate 9, and the locking mechanism is used to limit the position between the movable plate 9 and the pressing plate 10 to avoid separation of the movable plate 9 and the pressing plate 10, thereby playing a role in stable sealing, the locking mechanism includes a first connecting plate 12, and the first connecting plate 12 is fixedly connected to both ends of the pressing plate 10, the upper ends of both ends of the movable plate 9 are fixedly connected to the second connecting plate 13, and the positions of the first connecting plate 12 and the second connecting plate 13 correspond, and a limiting rod 14 is fixedly connected at the middle position of the bottom end of the first connecting plate 12, and the limiting rod 14 passes through and extends to the bottom of the second connecting plate 13.

[0042] A fixing groove 15 is formed on one side of the limiting rod 14 , and a limiting block 17 is fixedly connected to the inside of the fixing groove 15 via a connecting spring 16 . One side of the limiting block 17 passes through and extends to the outside of the limiting rod 14 .

[0043] The fixing groove 15 is connected to the connecting spring 16 , and the connecting spring 16 and the limiting block 17 are welded into an integrated structure.

[0044] In this example, after the sealing layer 4 is sealed, the positions of the first connecting plate 12 and the second connecting plate 13 are aligned, the limiting rod 14 is passed through the second connecting plate 13, and the limiting block 17 is popped out from the inside of the fixing groove 15 by the elastic force of the connecting spring 16. At the same time, the limiting block 17 limits the positions of the second connecting plate 13 and the first connecting plate 12. Since the height to which the movable plate 9 can move inside the air storage plate 8 is limited, after limiting the pressing plate 10 and the movable plate 9, the separation between the movable plate 9 and the pressing plate 10 due to arbitrary pulling out can be avoided, thereby making the sealing of the sealing layer 4 more secure.

[0045] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cooling device for cryopreservation of stem cells, wherein a top cover (2) is arranged at the upper end of a cooling box (1), and an adjustment plate (3) is fixedly connected to the bottom end of the top cover (2), and the adjustment plate (3) is snap-fitted and connected to the interior of the cooling box (1); a plurality of placement grooves (5) are provided at the top end of the cooling box (1), and the placement grooves (5) are connected through liquid nitrogen filling holes (6); and connecting pipes (7) are fixedly connected to both ends of the cooling box (1); Features: The cooling boxes (1) on both sides of the placement tank (5) are provided with gas storage tanks (21) inside, and a limiting mechanism is provided inside the gas storage tank (21), and the limiting mechanism is used to limit the position of the cell freezing tank, so that the cell freezing tank can be placed more stably; A sealing layer (4) is provided at the upper end of the inner side of the cooling box (1), and sealing mechanisms are provided on both sides of the outer side of the cooling box (1). The sealing mechanisms are used to inflate the sealing layer (4) to achieve sealing, thereby preventing the loss of liquid nitrogen temperature and playing a role of sealing and heat preservation.

2. A cooling device for cryopreservation of stem cells according to claim 1, characterized in that: The sealing mechanism comprises an air storage plate (8), and the air storage plate (8) is fixedly connected to one end of both sides of the cooling box (1), the air storage plate (8) is slidably connected to a movable plate (9) inside, one end of both sides of the bottom end of the top cover (2) is fixedly connected to a pressing plate (10), and the pressing plate (10) is arranged above the movable plate (9), and the lower end of one side of the air storage plate (8) is fixedly connected to a first hose (11), and the first hose (11) is connected to the sealing layer (4).

3. A cooling device for cryopreservation of stem cells according to claim 2, characterized in that: Both ends of the pressing plate (10) and the movable plate (9) are provided with locking mechanisms, and the locking mechanisms are used to limit the position between the movable plate (9) and the pressing plate (10) to prevent the movable plate (9) and the pressing plate (10) from being separated, thereby playing a role of stable sealing, the locking mechanism comprises a first connecting plate (12), and the first connecting plate (12) is fixedly connected to the two ends of the pressing plate (10), the upper ends of the two ends of the movable plate (9) are fixedly connected to the second connecting plate (13), and the positions of the first connecting plate (12) and the second connecting plate (13) correspond, and a limiting rod (14) is fixedly connected at the middle position of the bottom end of the first connecting plate (12), and the limiting rod (14) passes through and extends to the bottom of the second connecting plate (13).

4. A cooling device for cryopreservation of stem cells according to claim 3, characterized in that: A fixing groove (15) is provided on one side of the limiting rod (14), and the interior of the fixing groove (15) is fixedly connected to a limiting block (17) via a connecting spring (16), and one side of the limiting block (17) passes through and extends to the outside of the limiting rod (14).

5. A cooling device for cryopreservation of stem cells according to claim 4, characterized in that: The fixing groove (15) and the connecting spring (16) are connected, and the connecting spring (16) and the limiting block (17) are welded into an integrated structure.

6. A cooling device for cryopreservation of stem cells according to claim 1, characterized in that: The limiting mechanism comprises an airbag (19), and the airbag (19) is arranged inside the placement groove (5), a movable block (18) is fixedly connected above the airbag (19), and the movable block (18) and the placement groove (5) are slidably connected, a second hose (20) is fixedly connected to one side of the airbag (19), and one side of the second hose (20) passes through and extends to the inside of the air storage groove (21), a piston rod (22) is slidably connected to the inside of the air storage groove (21), and a rotating rod (23) is hinged at the top end of the piston rod (22).

7. A cooling device for cryopreservation of stem cells according to claim 6, characterized in that: The rotating rod (23) is arranged above the placement groove (5), and the rotating rod (23) is symmetrical with respect to the central axis of the placement groove (5) in the vertical direction.

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