Automatic liquid filling mechanism and system

Abstract

The utility model discloses an automatic liquid filling mechanism and system, the filling mechanism comprises a public guide rail, and a first span beam and a second span beam which are connected with the public guide rail, and two sides of the first span beam are provided with a consumable clamping unit and a magnetic bar sleeve extracting and loading unit which are connected with each other; a tubular clamping unit and a pipetting unit which are connected are arranged on the two sides of the second span beam, the third span beam is provided with a filling assembly, the filling assembly comprises a filling unit, and a third span beam driving mechanism can drive the filling assembly to move on the third span beam. The device further comprises a consumable receiving unit, a to-be-filled liquid consumable container, a fluid transmission channel, a filling lifting driving mechanism and a liquid driving pump which are associated with one another. According to the automatic liquid filling mechanism disclosed by the utility model, a plurality of functional components are integrally arranged and are respectively driven by the moving span beams, so that the system can intensively execute construction operation of an extraction system in a limited space, and the problem of dispersed multi-step operation caused by complicated operation of the extraction system is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical equipment, and in particular relates to an automatic liquid filling mechanism and system. Background Art

[0002] With the improvement of human quality of life, health has become the most concerned topic for the public. The development of medical technology allows people to grasp their own health status more quickly and timely. In smart terminals, wearable devices such as smart watches from companies such as Huawei, Xiaomi and Apple are increasingly integrating different types of detection units, such as pressure-sensitive sensor units, electrode sensor units, etc. to obtain heart rate, blood sugar and other health-related parameters. The application expansion of these devices also meets the overall needs of precision medicine. Molecular diagnostic technology that analyzes genetic material nucleic acid fragments has the characteristics of being able to detect targets in the early stages of infection and can provide disease warnings as early as possible, so that it can guide or intervene in treatment as early as possible. It can also be used to guide precise medication to efficiently assist different people in personalized medication to achieve the goal of early recovery. Of course, molecular diagnostic technology is also widely used in other scenarios, which makes molecular diagnosis an important detection tool in modern medical diagnosis. In recent years, the number of targets for molecular diagnosis has increased, including exogenous pathogenic factors such as infections caused by bacteria, viruses or other microorganisms, endogenous pathogenic factors such as normal cell canceration, mutations in genetic gene fragments themselves, such as differences in alcohol metabolism or differences in drug sensitivity for the same disease, etc. These can be detected in a timely manner through qualitative or quantitative detection of the root genetic material. With the in-depth research of various institutions, more and more diseases can be explained by mutations in genetic material, such as Alzheimer's disease. Therefore, more and more tests are being conducted using secretions, body fluids, blood and various types of swab preservation fluids as samples. In order to meet the needs of large-scale and rapid processing of various samples, Different manufacturers have developed different types of integrated systems, among which the pipeline processing system has the advantages of high automation, low manual involvement and high detection efficiency. However, there are relatively few batch sample processing systems for molecular diagnosis. Among them, Abbott, Roche and Beckman have corresponding pipeline processing systems, which have gradually entered the Chinese market in recent years. Overall, since the equipment needs to complete all functions of sample processing, nucleic acid extraction, amplification system establishment and amplification detection in a limited space, the system needs to have characteristics such as high efficiency, integration and low pollution. Among them, nucleic acid extraction is an important part of molecular diagnosis, and the on-site configuration of reagents does not need to consider the validity period of the reagents and can also meet the requirements of efficient and convenient transportation.

[0003] In the solution authorized by the U.S. invention patent US8951805B2, the liquid reagent containers to be filled are arranged in parallel, and the liquid level in the filling container can be monitored in real time by using sensors. When it is detected that the liquid volume in the filling container is less than the preset value, the pump can be started to pump the corresponding liquid to be filled in another container into the filling container, which can achieve rapid filling liquid replenishment and make the entire filling operation more continuous and efficient. The solution authorized by US8715574B2 focuses on the filling control method for protecting the entire system; the Chinese invention patent CN103884851B authorizes a solution that can automatically fill reagents, wherein the liquid reagent to be filled is stored in a large-volume barrel form and can be loaded in batches, and a plurality of corresponding string vibration sensors are configured on the barrel reagent storage position to accurately sense the weight change of the barrel reagent, thereby achieving the goal of accurately controlling the amount of liquid to be filled, and the reagent storage barrel is improved and designed to better meet the on-site reagent filling needs. However, the above modules are generally designed to be scattered, and some special reagents are not suitable for pumping filling solutions. For example, the magnetic bead preservation solution is essentially a solid-liquid two-phase state. To ensure the extraction efficiency, it is necessary to ensure that the magnetic bead preservation solution can be fully mixed before transfer. In this way, accurate weighing of the pumped reagent barrel cannot be achieved. In addition, the small amount of reagent filling itself is easy to accumulate and clog in the liquid system. At the same time, there are particularly high requirements for high-precision pumping equipment. This requires the establishment of the extraction system itself to require the coordinated work of multiple functional units, which is a great challenge to the integrated design itself.

[0004] In in vitro diagnostic technology, automated processing equipment with a higher degree of integration needs to meet the requirements of on-site configuration of nucleic acid extraction reaction systems and PCR amplification reaction systems. This design has also been adopted by various types of molecular diagnostic equipment that have been developed. However, there are still many problems in the actual operation process. For example, many reaction processes require the participation of magnetic beads, which usually exist in a two-phase state of solid-liquid suspension. There are certain technical problems with the pumping scheme of this reagent. Roche has developed an agitator that can be configured in a magnetic bead barrel to stir the suspension, which can ensure the suspension and mixing state of the magnetic beads to a certain extent. However, pumping this liquid also needs to take into account the special requirements of precision and the physical properties of the pumping medium itself. Therefore, there are high requirements for the precision of the pumping equipment and the liquid system, which leads to an increase in design costs. There are also many risk points of blockage in the pumping equipment and the liquid system, which makes the system less reliable. Therefore, the utility model designs a combination module that can include an automated liquid filling module to solve the specific problems of special medium transfer that cannot be solved by a simple pumping system. Utility Model Content

[0005] In order to solve the above problems existing in the prior art, the utility model designs the following technical solutions:

[0006] In a first aspect of the utility model, an automated liquid filling mechanism is provided, comprising a common guide rail, a first span beam connected to the common guide rail, and a second span beam, wherein the common guide rail is provided with a first drive assembly and a second drive assembly, the first drive assembly and the second drive assembly can respectively drive the first span beam and the second span beam to move on the common guide rail; the first span beam is provided with a first span beam driving mechanism, and both sides of the first span beam are respectively provided with connected consumable clamping units and extraction magnetic rod sleeve loading units, the first span beam driving mechanism can drive the consumable clamping unit and the extraction magnetic rod sleeve loading unit to move along the first span beam; the second span beam is provided with a second span beam driving mechanism, the second span beam is provided with a second span beam driving mechanism, Connected tubular clamping units and pipetting units are respectively arranged on both sides of the span beam, and the second span beam driving mechanism can drive the tubular clamping unit and the pipetting unit to move on the second span beam, and the tubular clamping unit can clamp and transfer the reagent bottle and open and close the lid of the reagent bottle; it also includes a third span beam, and the third span beam is provided with a third span beam driving mechanism and a filling assembly, and the third span beam driving mechanism can drive the filling assembly to move on the third span beam, and the filling assembly includes more than one filling unit, and the filling unit is fluidically connected to the liquid consumable container to be filled, the fluid transmission channel and the liquid drive pump; it also includes a filling lifting drive mechanism that can adjust the vertical position of the filling unit.

[0007] In one embodiment of the utility model, the consumable clamping unit can clamp the consumable unit at the first preset position and transfer it as a consumable receiving unit, the tubular clamping unit transfers the reagent bottle at the second preset position to the third preset position and opens the cover, the pipetting unit transfers the reagent in the reagent bottle at the third preset position to the consumable receiving unit, the filling lifting drive mechanism drives the filling unit to the top of the consumable receiving unit, the liquid driving pump transfers the liquid to be filled in the liquid consumable container to be filled through the fluid transmission channel to the filling unit and then pumps it to the consumable receiving unit, and the extraction magnetic rod sleeve loading unit moves to the consumable receiving unit to complete the magnetic rod sleeve configuration.

[0008] In one embodiment of the utility model, it also includes a cleaning liquid consumable container and a cleaning tank. The filling lifting drive mechanism can drive the filling unit to the top of the cleaning tank. The liquid drive pump transfers the cleaning liquid in the cleaning liquid consumable container to the filling unit through the fluid transmission channel to complete the internal and external cleaning of the filling unit.

[0009] In one embodiment of the utility model, the second span beam driving mechanism includes a second span beam driving motor, a second span beam driving wheel, a second span beam driven wheel, a second span beam transmission belt and a second span beam connecting block. The second span beam driving motor is arranged at the top of the second span beam. The output shaft of the second span beam driving motor is connected to the second span beam driving wheel. The second span beam driven wheel is arranged at a preset interval from the second span beam driving wheel. The second span beam transmission belt is wound between the second span beam driving wheel and the second span beam driven wheel. The second span beam connecting block is connected to one side of the second span beam transmission belt. A second span beam slide rail is arranged along the length direction of the second span beam. The second span beam connecting block is provided with an interlocking groove. The second span beam connecting block is slidably connected to the second span beam slide rail.

[0010] In one embodiment of the utility model, the pipetting unit is further configured with a liquid receiving assembly, which includes a liquid receiving plate driving motor, a liquid receiving driving gear, a liquid receiving transmission rack and a liquid receiving plate. The liquid receiving plate driving motor is connected to the liquid receiving driving gear, the liquid receiving driving gear is meshingly connected to the liquid receiving transmission rack, and the liquid receiving transmission rack is fixedly connected to the liquid receiving plate. When the liquid receiving plate driving motor rotates, the liquid receiving transmission rack can drive the liquid receiving plate to the bottom of the pipetting unit to receive dripping droplets.

[0011] In one embodiment of the utility model, the third span beam driving mechanism includes a third span beam driving motor, a third span beam driving wheel, a third span beam driven wheel, a third span beam transmission belt and a third span beam connecting block, wherein the output shaft of the third span beam driving motor is connected to the third span beam driving wheel, a third span beam driven wheel is arranged at a preset interval from the third span beam driving wheel, a third span beam transmission belt is wound between the third span beam driving wheel and the third span beam driven wheel, a third span beam connecting block is connected to one side of the third span beam transmission belt, and the third span beam connecting block is slidably connected to a third span beam slide rail extending in the length direction of the second span beam.

[0012] In one embodiment of the present invention, the configuration heights of the first span beam and the second span beam are higher than the configuration height of the third span beam, and the third span beam is fixedly configured.

[0013] In one embodiment of the utility model, the extraction magnetic rod sleeve loading unit is provided with a loading drive motor, a loading transmission screw, a first loading connection block, a second loading connection block, a loading transmission slide rail and one or more magnetic rod sleeve capture units, wherein the loading drive motor can drive the loading transmission screw to rotate, so that the first loading connection block connected to the loading transmission screw moves along the length direction of the loading transmission screw, the loading transmission slide rail is arranged in the vertical direction of the extraction magnetic rod sleeve loading unit, the second loading connection block is engaged and connected to the loading transmission slide rail, and the second loading connection block is connected to the extraction magnetic rod sleeve loading unit.

[0014] In one embodiment of the utility model, the extraction magnetic rod sleeve loading unit is also provided with a separation structure and a separation drive motor, the separation structure comprises a groove structure adapted to clamp the one or more magnetic rod sleeve acquisition units, the separation structure can be driven by the separation drive motor to be inserted into the combination position of the magnetic rod sleeve acquisition unit and the magnetic rod sleeve through lifting movement, and the separation structure can abut and separate the magnetic rod sleeve through the relative movement of lifting and lowering.

[0015] In the second aspect of the utility model, an automated liquid filling system is provided, including the automated liquid filling mechanism, and also including an extraction consumables carrying position, a pipetting consumables storage position, a magnetic rod box storage position, a transmission channel and a magnetic bead oscillation area, wherein the extraction consumables carrying position, the pipetting consumables storage position and the magnetic rod box storage position are all located at the lower part of the first span beam and the second span beam, and an extraction consumables fitting groove is arranged at the bottom of the extraction consumables carrying position, and the extraction consumables fitting groove can be slidably connected to the extraction consumables slide rail, and the extraction consumables carrying position can be driven by the extraction consumables driving mechanism to slide along the extraction consumables slide rail, and the extraction consumables driving mechanism includes an extraction consumables driving motor and a belt A wheel transmission mechanism, the output of the extraction consumables driving motor is connected to the pulley transmission mechanism, the pulley transmission mechanism includes an extraction consumables transmission belt and an extraction consumables connecting block, one side of the extraction consumables transmission belt can be fixedly connected to the extraction consumables bearing position by the extraction consumables connecting block, the transmission channel is provided with a transmission channel interaction port, the magnetic rod box storage position, the first type of pipetting consumables storage position and the second type of pipetting consumables storage position are all arranged close to the transmission channel, the transmission channel can receive different types of consumables and transfer the consumables out of the automated liquid filling system through the transmission channel interaction port, an oscillation driving mechanism is provided in the magnetic bead oscillation area, and the oscillation driving mechanism can perform a mixing operation on the magnetic bead liquid.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] 1. The automatic liquid filling mechanism provided by the utility model includes two moving spans that can be driven to move and one fixed span, and different functional components are configured on both sides of the width direction of the two moving spans. The fixed span is connected to the filling unit, and the filling unit can automatically perform pumping liquid filling into the target consumables. By adaptively adjusting the type of liquid that can be filled, other types of fluids that are not suitable for pumping filling are independently filled using different functional components on the two moving spans, and other functional components are also configured on the two moving spans to ensure that the entire module is more integrated. One side of one of the moving spans is configured with a consumable clamping unit, which is connected to the consumable clamping unit. An extraction magnetic rod sleeve loading unit is set on the other side, and the two are connected to each other and can be driven simultaneously, so that the span beam can perform consumables clamping and transfer and extraction magnetic rod sleeve loading within the travel range, and the two sides of the other span beam include tubular clamping units, and the other side opposite to it includes at least one pipetting unit, so that the two span beams perform different division of labor operations, and the two span beams can perform clamping and transfer, pipetting and addition and other functions with minimal interference in independent areas with less overlap. In order to ensure that the pipetting has higher efficiency and minimize the differentiated requirements of different types of liquids for pipetting volume and accuracy, a pipetting unit is also configured on the same configuration side of the tubular clamping unit.

[0018] 2. In the automatic liquid filling mechanism provided by the utility model, the first span beam includes a first span beam driving motor, whose output shaft is connected to the first span beam driving wheel, and a first span beam driven wheel is arranged at a preset interval from the first span beam driving wheel, and a first span beam transmission belt is wound between the first span beam driving wheel and the first span beam driven wheel, and the first span beam transmission belt can be connected to the consumable clamping unit or the magnetic rod sleeve loading unit through the first span beam connecting block, and the consumable clamping unit and the magnetic rod sleeve loading unit can be synchronously driven to move along the length direction of the first span beam through the pulley transmission system. The tubular clamping unit and the liquid transfer unit on the second span beam can be driven to move along the length direction of the second span beam, thereby simplifying the motion drive of different functional components, and one end of the first span beam and the second span beam in the length direction are respectively connected to the common guide rail by the first slider and the second slider, and the other end opposite to them in the length direction is respectively configured on the common guide rod by the roller group, thereby ensuring that the motion reference and motion accuracy of the moving span beam are the same, making the motion drive of the span beam simpler and more reliable.

[0019] 3. The automated liquid filling system provided by the utility model includes an automated liquid filling mechanism, a pipetting consumables storage position, an extraction magnetic rod sleeve storage position, a magnetic bead liquid oscillation position and a transmission channel. The first span beam and / or the second span beam can be driven to move between different operating positions to perform different operations. Filling units and cleaning tanks are arranged at intervals on both sides of the length direction of the third span beam. The filling unit can be driven by the filling lifting drive mechanism to move to different operating positions to perform liquid filling or cleaning operations in the target consumables. The various operating positions in the automated liquid filling system can perform the construction and configuration of the extraction system in a highly integrated manner with the cooperation of two movable span beams and one fixed span beam.

[0020] 4. The automated liquid filling system provided by the utility model has multiple functional components with an integrated layout, and each of them is driven by a moving cross-beam, so that the system can centrally perform the construction operation of the extraction system in a limited space, solving the problem of dispersed multi-step operations caused by the complicated operation of the extraction system, and can also quickly construct the extraction reaction system with high efficiency and low pollution.

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of a side view of the automatic liquid filling mechanism provided by an embodiment of the utility model;

[0023] Figure 2 is a structural schematic diagram of the automated liquid filling mechanism provided by an embodiment of the utility model from another side view;

[0024] Figure 3 yes Figure 1 A schematic diagram of the structure of the extraction magnetic rod sleeve loading unit of the automated liquid filling mechanism;

[0025] Figure 4 It is a structural schematic diagram of the automated liquid filling system provided by an embodiment of the utility model;

[0026] Figure 5 yes Figure 4 A schematic diagram of the state of the consumable clamping unit in the automated liquid filling system when transferring consumables;

[0027] Figure 6 yes Figure 4 Schematic diagram of the status of the filling unit in the automated liquid filling system when filling liquid.

[0028] Description of reference numerals:

[0029] 10-first span beam; 11-first configuration module; 110-consumable material clamping unit; 111-clamping unit connecting block; 1101-first clamping jaw; 1102-second clamping jaw; 12-second configuration module; 120-extraction magnetic rod sleeve loading unit; 1201-magnetic rod sleeve acquisition unit; 121-loading drive motor; 122-loading transmission screw rod; 123-first loading connection block; 124-second loading connection block; 125-loading transmission slide rail; 126-limit sensor; 127-separation structure; 131-first span beam driving motor; 132-first span beam driving wheel; 133-first span beam transmission belt; 134-first span beam slide rail; 135-first span beam connecting block;

[0030] 20-second span beam; 21-third configuration module; 22-fourth configuration module; 210-tubular clamping unit; 220-liquid transfer unit; 221-liquid transfer lifting drive motor; 222-liquid transfer lifting drive wheel; 223-liquid transfer lifting drive belt; 224-liquid receiving tray drive motor; 225-liquid receiving drive gear; 226-liquid receiving drive rack; 227-liquid receiving tray; 231-second span beam drive motor; 232-second span beam drive wheel; 233-second span beam drive belt; 234-second span beam slide rail; 235-second span beam connecting block;

[0031] 30-third span beam; 31-filling assembly; 310-filling unit; 311-filling lifting drive motor; 312-filling lifting drive belt; 313-filling lifting driven wheel; 321-pressure sensing unit; 331-third span beam drive motor; 332-third span beam drive wheel; 333-third span beam drive belt; 334-third span beam slide rail; 335-third span beam connecting block;

[0032] 401-first slider; 402-second slider slides; 403-common guide rail, 404-fixed connection block; 405-common guide rod; 411-first drive motor; 412-second drive motor;

[0033] 50-extraction consumables carrying position; 501-extraction consumables fitting groove; 502-extraction consumables slide rail; 503-extraction consumables connecting block; 504-extraction consumables transmission belt; 51-first type of pipetting consumables storage position; 52-second type of pipetting consumables storage position; 53-magnetic rod box storage position; 541-linked tube amplification consumables receiving drawer unit; 542-deep well plate consumables receiving drawer unit; 543-first pipetting consumables receiving drawer unit; 544-second pipetting consumables receiving drawer unit; 551-first reagent carrying position; 552-second reagent carrying position; 553-magnetic bead reagent carrying position; 56-transmission channel; 561-transmission channel interaction port; 57-pipetting operation area; 58-magnetic bead oscillation area;

[0034] 601 - first visual sensor; 602 - second visual sensor. DETAILED DESCRIPTION

[0035] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the present invention, the scheme according to the present invention is described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0036] The above and other technical contents, features and effects of the utility model are clearly presented in the following detailed description of the specific implementation mode in conjunction with the accompanying drawings. Through the description of the specific implementation mode, the technical means and effects adopted by the utility model to achieve the predetermined purpose can be more deeply and specifically understood. However, the attached drawings are only for reference and explanation, and are not used to limit the technical solution of the utility model.

[0037] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants are intended to cover non-exclusive inclusion, so that an article or device including a series of elements includes not only those elements, but also other elements that are not explicitly listed. In the absence of more restrictions, the elements defined by the statement "including one..." do not exclude the existence of other identical elements in the article or device including the elements.

[0038] Embodiment 1

[0039] Please refer to Figure 1 and Figure 2 , Figure 1 This is a schematic diagram of the structure of the automatic liquid filling mechanism provided by the embodiment of the utility model from a side view. Figure 2 It is a structural schematic diagram of the automated liquid filling mechanism provided by an embodiment of the utility model from another side view, wherein the automated liquid filling mechanism includes a common guide rail 403 , a first span beam 10 and a second span beam 20 connected to the common guide rail 403 .

[0040] Furthermore, a first driving assembly and a second driving assembly are also provided on the common guide rail 403 side, and the first driving assembly and the second driving assembly can respectively drive the first span beam 10 and the second span beam 20 to move on the common guide rail 403; the first span beam 10 is provided with a first span beam driving mechanism, and the two sides of the first span beam 10 are respectively provided with a connected consumable clamping unit 110 and an extraction magnetic rod sleeve loading unit 120, and the first span beam driving mechanism can drive the consumable clamping unit 110 and the extraction magnetic rod sleeve loading unit 120 to move along the first span beam; the second span beam 20 is provided with a second span beam driving mechanism, and the two sides of the second span beam 20 are respectively provided with a connected tubular clamping unit 210 and a pipetting unit 220, and the second span beam driving mechanism can drive the tubular clamping unit 210 and the pipetting unit 2 20 moves on the second span beam, and the tubular clamping unit 210 can clamp and transfer the reagent bottle and open / close the lid of the reagent bottle; the automated liquid filling mechanism also includes a third span beam 30, which is optimally fixed here and also ensures the reliability of the system. The third span beam 30 is provided with a third span beam driving mechanism and a filling assembly 31, and the third span beam driving mechanism is fixed by a fixed connecting block 404. The third span beam driving mechanism can drive the filling assembly 31 to move on the third span beam 30, and the filling assembly 31 includes more than one filling unit 310, and the filling unit 310 is fluidically connected to the liquid consumable container to be filled, the fluid transmission channel and the liquid drive pump. The automated liquid filling mechanism also includes a filling lifting drive mechanism that can adjust the vertical position of the filling unit 310.

[0041] Furthermore, the consumable clamping unit 110 can clamp and transfer the consumable unit at the first preset position as a consumable receiving unit. The consumable clamping unit 110 is provided with two opposing jaws, namely a first jaw 1101 and a second jaw 1102, which can clamp and transfer extraction deep well plate consumables, amplification tube consumables, etc. The tubular clamping unit 210 transfers the reagent bottle at the second preset position to the third preset position and opens the cover (a reagent bottle clamping mechanism is configured in the third preset position, which can apply a clamping force to the reagent bottle body and open or snap the reagent bottle with the cooperation of the tubular clamping unit 210), the pipetting unit 220 transfers the reagent in the reagent bottle at the third preset position to the consumable receiving unit, the filling lifting drive mechanism drives the filling unit 310 to the top of the consumable receiving unit, the liquid driving pump transfers the liquid to be filled in the liquid consumable container to be filled through the fluid transmission channel to the filling unit and then pumps it to the consumable receiving unit, the extraction magnetic rod sleeve loading unit 120 moves to the consumable receiving unit to complete the magnetic rod sleeve configuration (the extraction magnetic rod sleeve loading unit 120 can take the magnetic rod sleeve from the magnetic rod sleeve box storage position 53, which is shown here as taking 8 at a time, and transfers it to the consumable receiving unit to complete the magnetic rod sleeve configuration).

[0042] Further, the filling and lifting driving mechanism includes a filling and lifting driving motor 311, a filling and lifting transmission belt 312, a filling and lifting driving wheel, a filling and lifting connecting block, and a filling and lifting driven wheel 313. Specifically, the output of the filling and lifting driving motor 311 is connected to the filling and lifting driving wheel, the filling and lifting driving wheel is arranged with a preset interval in the vertical direction, the filling and lifting driving wheel is wound with a filling and lifting transmission belt 312, and one side of the filling and lifting transmission belt 312 is connected to the filling unit 310 through the filling and lifting connecting block. Subsequently, multiple filling units 310 are aligned with the corresponding number of receiving holes of the consumable receiving unit, and then the filling lifting drive motor 311 drives the filling unit 310 to descend, and the filling unit 310 fills the holes of the consumable receiving unit with reagents. After completing the reagent filling of multiple holes, the consumable receiving unit can be driven to change its position so that the holes of another part of the reagents to be received are aligned with several filling units, and this is repeated until all the holes of the consumable receiving unit are filled with various reagents. Finally, the magnetic bead liquid is extracted. In this embodiment, the magnetic bead liquid is not pumped and filled by the filling unit 310, but is transferred separately from the oscillation mixing by the pipetting unit 220. This can avoid the problems of difficulty in mixing the magnetic beads, difficulty in controlling the filling amount, and easy clogging of the filling system. Of course, other types of reagents required for the extraction system, such as eluents, can also be filled separately using a pipette.

[0043] Preferably, the automated liquid filling mechanism provided in this embodiment also includes a cleaning liquid consumable container and a cleaning tank, and the filling lifting drive mechanism can drive the filling unit 310 to the top of the cleaning tank, and the liquid driving pump transmits the cleaning liquid in the cleaning liquid consumable container to the filling unit through the fluid transmission channel to complete the internal and external cleaning of the filling unit. After performing a certain number of reagent fillings, the filling lifting drive motor 311 drives the filling unit 310 to move directly above the cleaning tank, and cleans the inside and outside of the filling unit 310 to ensure that the system can always efficiently and accurately fill the reagent. The automated liquid filling mechanism provided in this embodiment can effectively reduce the occurrence of reliability problems such as salting out blockage or corrosion of the filling system by other components. The third span beam driving mechanism can drive the filling unit 310 to move along the length direction of the third span beam 30, so that fewer filling units 310 can be used to perform the filling of reagents in all holes of the consumable receiving unit.

[0044] In order to effectively monitor the operating status of the filling unit 310 in real time, the filling unit 310 is also provided with a pressure sensing unit 321. The number of the pressure sensing units 321 is the same as the number of the filling units 310. In this way, intermittent or continuous monitoring of the pressure of each filling unit 310 and its fluidically connected pipelines can be achieved to determine whether the various components of the system are operating normally or whether there is any pipeline blockage, etc.

[0045] Further, the automatic liquid filling mechanism includes a common guide rail 403, and two movable span beams that can be driven to move are arranged along the length direction of the common guide rail 403, namely, a first span beam 10 and a second span beam 20, and the length direction of the common guide rail 403 is the transverse direction. One end of the first span beam 10 in the length direction is slidably connected to the common guide rail 403 by the first slider 401, and the other end of the first span beam 10 in the length direction can be rollingly connected to the common guide rod 405 by the first roller group. Specifically, the first driving assembly includes a first driving motor 411 and a driving transmission mechanism, and the driving transmission mechanism can be a pulley transmission mechanism, a sprocket transmission mechanism, or a gear rack transmission mechanism. A consumable clamping unit 110 is arranged on one side of the two sides in the width direction of the first span beam 10, and an extraction magnetic rod sleeve loading unit 120 is arranged on the other side opposite to the consumable clamping unit 110. The first configuration module 11 of the consumable clamping unit 110 is fixedly connected to the second configuration module 12 of the extraction magnetic rod sleeve loading unit 120 through a bridge member. Such an arrangement enables the two configuration modules on both sides of the width direction of the first span beam 10 to be driven as a whole, thereby simplifying the drive system. Since the first span beam 10 includes the consumable clamping unit 110 and the extraction magnetic rod sleeve loading unit 120, the consumable clamping unit 110 and the extraction magnetic rod sleeve loading unit 120 are respectively used for the front end preparation of the consumable transfer before the PCR reaction, and for the subsequent transfer of the magnetic rod sleeve extraction. Therefore, arranging the two functional units of the consumable clamping unit 110 and the extraction magnetic rod sleeve loading unit 120 on the same span beam can make the movement range of the first span beam smaller, and the efficiency of operations such as transfer and loading is also higher.

[0046] Furthermore, a first span beam driving mechanism is configured in the length direction of the first span beam 10, which can synchronously drive the consumable clamping unit 110 and the extraction magnetic rod sleeve loading unit 120 to move, and the first span beam driving mechanism includes a first span beam driving motor 131, a first span beam driving wheel 132, a first span beam driven wheel, a first span beam transmission belt 133 and a first span beam connecting block 135. Specifically, the first span beam driving motor 131 is arranged at the top of the first span beam 10, and the output shaft of the first span beam driving motor 131 is connected to the first span beam driving wheel 132, and the first span beam driven wheel is configured at a preset interval from the first span beam driving wheel 132, and the first span beam driving wheel 132 and the first span beam driven wheel are wound with a first span beam transmission belt 133, and the first span beam transmission belt 133 can be connected to the consumable clamping unit 110 or the magnetic rod sleeve loading unit 120 through the first span beam connecting block 135. In this embodiment, the first span beam connecting block 135 is connected to the configuration module 12 of the magnetic rod sleeve loading unit 120. Since the consumable clamping unit 110 and the magnetic rod sleeve loading unit 120 are connected, the clockwise or counterclockwise rotational motion of the first span beam driving motor 131 can be converted into sliding motion of the consumable clamping unit 110 and the magnetic rod sleeve loading unit 120.

[0047] Preferably, the consumable clamping unit 110 is connected to the first span beam 10 through the clamping unit connecting block 111, and a first span beam slide rail 134 is arranged along the length direction of the first span beam 10. The first span beam connecting block 135 is provided with an interlocking groove, which is slidably connected to the first span beam slide rail 134, so that the sliding resistance of the first span beam connecting block 135 is reduced, thereby improving the stability of the sliding movement of the consumable clamping unit 110 and the magnetic rod sleeve loading unit 120.

[0048] Similarly, one end of the second span beam 20 in the length direction can be slidably connected to the common guide rail 403 by the second slider 402, and the other end of the second span beam 20 in the length direction can be rollingly connected to the common guide rod 405 by the second roller group. The second driving assembly includes a second driving motor 412 and the driving transmission mechanism. The first span beam 10 and the second span beam 20 can be driven by the first driving motor 411 and the second driving motor 412 through the driving transmission mechanism, respectively, so that when the first driving motor 411 and the second driving motor 412 rotate clockwise or counterclockwise, the first span beam 10 and the second span beam 20 are driven to move along the extension direction of the common guide rail 403. One side of the two sides in the width direction of the second span beam 20 is set as a tubular clamping unit 210, and the other side opposite to the tubular clamping unit 210 is set with at least one pipetting unit 220. In this embodiment, the pipetting unit is one, and the third configuration module 21 of the tubular clamping unit and the fourth configuration module 22 of the pipetting unit are also fixedly connected by a bridge member. The tubular clamping unit 210 is used to open and close the lid of the reagent tube. The tubular clamping unit 210 cooperates with the pipetting unit 220 to perform the pipetting operation of the reagent tube. The two functional units are arranged on the same span beam, which can minimize the time of opening and closing the lids of various reagents, and can also enable the cover opening and closing and the pipette to perform operations within a smaller range of travel. The configuration of different functional units on both sides of the first span beam 10 and the second span beam 20 can ensure that the overlapping area of ​​the movement travel of the two span beams is small, and the two span beams can complete different operating functions without hindrance within a partial time sequence. In addition, the functional units on the first span beam 10 and the second span beam 20 also include a driving module that can drive the lifting motion thereof in the vertical direction. Here, the vertical motion drive of the pipetting unit 220 is used as an example for explanation. The output shaft of the pipetting lifting drive motor 221 is connected to the pipetting lifting drive wheel 222. A pipetting lifting driven wheel is arranged at a preset interval in the vertical direction. A pipetting lifting drive belt 223 is wound between the pipetting lifting drive wheel 222 and the pipetting lifting driven wheel. One end of the pipetting lifting drive belt 223 is connected to the pipetting unit 220. The other end of the pipetting lifting drive belt 223 can be connected to the pipetting lifting slide rail through a pipetting lifting connection block. When the pipetting lifting drive motor 221 drives the pipetting lifting drive belt 223, at least one pipetting unit 220 can be driven to lift and lower motion smoothly with low resistance. Similar to the vertical motion drive of the pipetting unit 220, other functional units can be driven to change their positions in three-dimensional space through the same setting.

[0049] Further, the second span beam 20 also includes a second span beam driving mechanism, which includes a second span beam driving motor 231, a second span beam driving wheel 232, a second span beam driven wheel, a second span beam transmission belt 233 and a second span beam connecting block 235. Specifically, the second span beam driving motor 231 is arranged at the top of the second span beam 20, the output shaft of the second span beam driving motor 231 is connected to the second span beam driving wheel 232, a second span beam driven wheel is arranged at a preset interval from the second span beam driving wheel 232, a second span beam transmission belt 233 is wound between the second span beam driving wheel and the second span beam driven wheel, and a second span beam connecting block 235 is connected to one side of the second span beam transmission belt 233. Preferably, a second span beam slide rail 234 is arranged along the length direction of the second span beam 20, an interlocking groove is arranged on the second span beam connecting block 235, and the second span beam connecting block 235 is slidably connected to the second span beam slide rail 234.

[0050] Furthermore, the liquid receiving unit 220 is also equipped with a liquid receiving assembly, which includes a liquid receiving plate driving motor 224, a liquid receiving driving gear 225, a liquid receiving transmission rack 226 and a liquid receiving plate 227. Specifically, the liquid receiving plate driving motor 224 is connected to the liquid receiving driving gear 225, the liquid receiving driving gear 225 is meshingly connected to the liquid receiving transmission rack 226, and the liquid receiving transmission rack 226 is fixedly connected to the liquid receiving plate 227. The liquid receiving plate driving motor 224 can drive the liquid receiving plate 227 to move to the bottom of the liquid receiving unit 220 to receive possible dripping drops, or retract to allow the liquid receiving unit 220 to perform a suction and pipetting operation, that is, when the liquid receiving plate driving motor rotates, the liquid receiving transmission rack can drive the liquid receiving plate to the bottom of the liquid receiving unit to receive dripping drops.

[0051] The automatic liquid filling mechanism provided in this embodiment includes two moving spans that can be driven to move and one fixed span, and different functional components are configured on both sides of the width direction of the two moving spans. The fixed span is connected to the filling unit, and the filling unit can automatically perform pumping liquid filling into the target consumable. By adaptively adjusting the type of liquid that can be filled, other types of fluids that are not suitable for pumping filling are independently filled using different functional components on the two moving spans. In addition, other functional components are also configured on the two moving spans to ensure that the entire module is more integrated. One side of one of the moving spans is configured with a consumable clamping unit, and the other opposite side is configured with a consumable clamping unit. An extraction magnetic rod sleeve loading unit is arranged on the side, and the two are connected to each other and can be driven simultaneously, so that the span beam can perform consumables clamping and transfer and extraction magnetic rod sleeve loading within the travel range, and the two sides of the other span beam include tubular clamping units, and the other side opposite to it includes at least one pipetting unit 220, so that the two span beams perform different division of labor operations, and the two span beams can perform clamping and transfer, pipetting and addition and other functions with minimal interference in independent areas with less overlap. In order to ensure that the pipetting has higher efficiency and minimize the differentiated requirements of different types of liquids for pipetting volume and accuracy, a pipetting unit 220 is also arranged on the same configuration side of the tubular clamping unit.

[0052] Furthermore, in the automated liquid filling mechanism provided in the present embodiment, the first span beam includes a first span beam driving motor, an output shaft of which is connected to the first span beam driving wheel, a first span beam driven wheel is arranged at a preset interval from the first span beam driving wheel, a first span beam transmission belt is wound between the first span beam driving wheel and the first span beam driven wheel, the first span beam transmission belt can be connected to the consumable clamping unit 110 or the magnetic rod sleeve loading unit 120 through the first span beam connecting block, and the consumable clamping unit 110 and the magnetic rod sleeve loading unit 120 can be synchronously driven to move along the length direction of the first span beam through the pulley transmission system. Similarly, the tubular clamping unit 210 and the pipetting unit 220 on the second span beam can be driven to move along the length direction of the second span beam, thereby simplifying the motion drive of different functional components. One end of the first span beam and the second span beam in the length direction are slidably connected to the common guide rail 403 through the first slider 401 and the second slider 402, respectively, and the other end opposite thereto in the length direction is rollingly configured on the common guide rod 405 through a roller group, thereby ensuring that the motion reference and motion accuracy of the moving span beam are the same, making the span beam motion drive simpler and more reliable.

[0053] Since the functional units on the first span beam 10 and the second span beam 20 can be driven to change their positions in three-dimensional space to prepare for the operation on the third span beam 30, the functional units on the third span beam 30 only need to change their positions in a certain two-dimensional space, and the travel path complexity of the entire system is relatively low. A filling unit 310 is disposed on one side of the third span beam 30 in the width direction.

[0054] Furthermore, when the third span beam 30 includes a plurality of filling units 310, more liquid can be pumped and filled efficiently. In order to ensure that the filling unit 310 can be driven to move in the length direction of the third span beam 30, a third span beam driving mechanism is provided on the third span beam 30. Preferably, the third span beam driving mechanism includes a third span beam driving motor 331, a third span beam driving wheel 332, a third span beam driven wheel, a third span beam transmission belt 333 and a third span beam connecting block 335. Specifically, the third span beam driving motor 331 is arranged at the top of the third span beam, the output shaft of the third span beam driving motor 331 is connected to the third span beam driving wheel 332, and the third span beam driven wheel is arranged at a preset interval from the third span beam driving wheel 332, and the third span beam transmission belt 333 is wound between the third span beam driving wheel 332 and the third span beam driven wheel, and the third span beam connecting block 335 is connected to one side of the third span beam transmission belt 333, and the third span beam connecting block 335 is slidably connected to the third span beam slide rail 334 extending in the length direction of the second span beam.

[0055] Preferably, the vertical configuration height of the first span beam 10 and the second span beam 20 is higher than the vertical configuration height of the third span beam 30, and the third span beam 30 is fixedly configured. Such a configuration enables the third span beam 30 to effectively avoid the moving first span beam 10 and second span beam 20 in space. The two moving span beams will basically not be disturbed by the fixed span beam during movement, thereby ensuring that the reliability of the operation of the entire combined module is higher.

[0056] Please refer to Figure 3 , Figure 3 yes Figure 1The structure diagram of the extraction magnetic rod sleeve loading unit of the automatic liquid filling mechanism is shown in FIG. 1 , wherein the extraction magnetic rod sleeve loading unit 120 is provided with a loading drive motor 121, a loading transmission screw 122, a first loading connection block 123, a second loading connection block 124, a loading transmission slide rail 125 and one or more magnetic rod sleeve collection units 1201. The loading drive motor 121 can drive the loading transmission screw 122 to rotate, so that the first loading connection block 123 connected to the loading transmission screw 122 is rotated along the length direction of the loading transmission screw 122. The first loading connection block 123 is connected to the second loading connection block 124, and the second loading connection block 124 is connected to the loading transmission slide rail 125. The second loading connection block 124 is also connected to the extraction magnetic rod sleeve loading unit 120. Preferably, the second loading connection block 124 is fixedly connected to the extraction magnetic rod sleeve loading unit 120, so that the loading drive motor 121 can drive the extraction magnetic rod sleeve loading unit 120 to move up and down with high precision. In this embodiment, there are 8 magnetic rod sleeve acquisition units 1201, and a limit sensor 126 is also provided in conjunction with the magnetic rod sleeve acquisition unit 1201. The limit sensor 126 is used to limit the magnetic rod sleeve acquisition unit 1201 during the lifting movement. The 8 magnetic rod sleeve acquisition units 1201 can perform synchronous loading of 8 extraction magnetic rod sleeves in a conventional type 96-hole extraction deep-well plate consumable. The 96-hole extraction deep-well plate consumable includes 16 extraction magnetic rod sleeves. In this way, the loading of the magnetic rod sleeves can be completed by performing two loadings of the magnetic rod sleeves. The loading drive motor 121 can output a rotation drive to drive the loading transmission screw 122 to rotate. The loading transmission screw 122 is threadedly connected to the extraction magnetic rod sleeve loading unit 120 through the first loading connection block 123.

[0057] Preferably, the extraction magnetic rod sleeve loading unit 120 is also provided with a separation structure 127 and a separation drive motor, and the separation structure 127 includes a groove structure adapted to engage the one or more magnetic rod sleeve acquisition units 1201. The extraction magnetic rod sleeve loading unit 120 can be driven to descend and extract the magnetic rod sleeve from the magnetic rod sleeve consumable box through the one or more magnetic rod sleeve acquisition units 1201, and be driven to move to the receiving position of the deep-well plate consumables. The separation structure 127 can be driven to insert the magnetic rod sleeve acquisition unit 1201 and the magnetic rod sleeve combination position through the lifting movement, and the separation structure 127 can abut and separate the magnetic rod sleeve through the relative movement of lifting. Preferably, after the filling unit 310 has loaded the reagent at the corresponding hole position of the extraction deep-well plate consumables, the extraction magnetic rod sleeve loading unit 120 performs the loading of the extraction magnetic rod sleeve to ensure that the reagent filling and the magnetic rod sleeve loading will not interfere with each other.

[0058] Embodiment 2

[0059] The utility model also provides an automatic liquid filling system, please refer to Figure 4 and Figure 5 , Figure 4 It is a structural schematic diagram of the automated liquid filling system provided by the embodiment of the utility model. Figure 5 yes Figure 4 Schematic diagram of the state of the consumable clamping unit in the automated liquid filling system when transferring consumables. The automated liquid filling system includes the automated liquid filling mechanism in Example 1, an extraction consumable carrying position 50, a pipetting consumable storage position, a magnetic rod box storage position 53, a transmission channel 56 and a magnetic bead oscillation zone 58. Specifically, the extraction consumable carrying position 50, the pipetting consumable storage position and the magnetic rod box storage position 53 are all located at the lower part of the first span beam 10 and the second span beam 20. The bottom of the extraction consumable carrying position 50 is provided with an extraction consumable fitting groove 501, and the extraction consumable fitting groove 501 is slidably connected to the extraction consumable slide rail 502. The extraction consumable carrying position 50 can be driven by the extraction consumable driving mechanism to slide along the extraction consumable slide rail 502. The extraction consumable driving mechanism includes an extraction consumable driving motor and a pulley transmission mechanism. The output of the extraction consumable driving motor is connected to the belt The transmission channel 56 is connected to the transmission channel 56 by a wheel transmission mechanism, and the wheel transmission mechanism includes an extraction consumable transmission belt 504 and an extraction consumable connecting block 503. One side of the extraction consumable transmission belt 504 can be fixedly connected to the extraction consumable carrying position 50 by the extraction consumable connecting block 503. The transmission channel 56 is provided with a transmission channel interaction port 561 (which can be configured in the middle area of ​​the module where the filling system is located), a magnetic rod box storage position 53, a first type of pipetting consumable storage position 51 and a second type of pipetting consumable storage position 52 are all arranged close to the transmission channel 56. The transmission channel 56 can receive different types of consumables and transfer the consumables out of the automated liquid filling system through the transmission channel interaction port 561. An oscillation drive mechanism is provided in the magnetic bead oscillation area 58, and the oscillation drive mechanism can perform a mixing operation on the magnetic bead liquid.

[0060] The automated liquid filling system also includes a cleaning liquid consumable container and a cleaning tank. The filling lifting drive mechanism can drive the filling unit 310 to the top of the cleaning tank. The liquid drive pump transfers the cleaning liquid in the cleaning liquid consumable container to the filling unit through the fluid transmission channel to complete the internal and external cleaning of the filling unit.

[0061] Specifically, in addition to the above-mentioned automated liquid filling mechanism, the automated liquid filling system also includes an extraction consumables carrying position 50, a pipetting consumables storage position and a magnetic rod box storage position 53. The extraction consumables carrying position 50, the pipetting consumables storage position and the magnetic rod box storage position 53 are all located below the first span beam 10 and the second span beam 20. Specifically, the pipetting consumables storage position includes a first type of pipetting consumables storage position 51 and a second type of pipetting consumables storage position 52, and the extraction consumables carrying position 50 is configured to be a type that can be driven to slide. An extraction consumables mating groove 501 is configured at the bottom of the extraction consumables carrying position 50, and the extraction consumables mating groove 501 can be slidably connected to an extraction consumables slide rail 502 extending a certain length. Here, the extraction consumables carrying position 50 can be driven by the extraction consumables driving mechanism to slide along the extraction consumables slide rail 502. The extraction consumables driving mechanism includes an extraction consumables driving motor and a pulley transmission mechanism. The output of the extraction consumables driving motor is connected to the pulley transmission mechanism. The pulley transmission mechanism includes an extraction consumables drive belt 504 and an extraction consumables connecting block 503. One side of the extraction consumables drive belt 504 can be fixedly connected to the extraction consumables carrying position 50 by the extraction consumables connecting block 503.

[0062] The extraction consumable transmission belt 504 can drive the extraction consumable carrier 50 to cooperate with different operating units in different areas to perform various functions. The following is an example of magnetic bead liquid extraction of empty deep-well plate extraction consumables: First, when the empty deep-well plate extraction consumables are transferred to the extraction consumable carrier 50, the extraction consumable driving mechanism first drives the extraction consumable carrier 50 to move to the bottom of the filling unit 310 as a consumable receiving unit, or the consumable unit of the extraction consumable carrier 50 can be clamped and transferred through the consumable clamping unit 110 as a consumable receiving unit, that is, the first preset position is Figure 5The extraction consumable carrier 50 is at its current position as shown, and then the extraction consumable carrier 50 can be driven to move to the bottom of the filling component 31, and then a plurality of filling units 310 are aligned with the receiving holes corresponding to the number of extraction consumables in the extraction consumable carrier 50, and then the filling lifting drive motor 311 drives the filling unit 310 to descend, and the filling unit 310 fills the extraction consumable holes with reagents, and after completing the reagent filling of a plurality of holes, the extraction consumable carrier 50 can be driven to change its position so that another part of the holes to be received with the reagents are aligned with several filling units, and this is repeated until all the holes of the deep well plate extraction consumables are filled with various reagents. Finally, the magnetic bead liquid is added. In this embodiment, the magnetic bead liquid is not pumped and filled by the filling unit 310, but is transferred separately from the shaking mixing by the pipetting unit 220. This can avoid the problems of difficulty in mixing the magnetic beads, difficulty in controlling the filling amount, and easy clogging of the filling system. Of course, other types of reagents required by the extraction system, such as eluents, can also be filled separately using a pipette. The automated liquid filling system provided in this embodiment can effectively reduce the occurrence of reliability problems such as salting out blockage or corrosion of the filling system by other components. The third span beam driving mechanism can drive the filling unit 310 to move along the length direction of the third span beam 30, so that fewer filling units 310 can be used to perform the filling of reagents in all wells of the extraction deep well plate consumables. After performing a certain number of reagent fillings, the filling lifting drive motor 311 drives the filling unit 310 to move to the top of the cleaning tank, and cleans the inside and outside of the filling unit 310 to ensure that the system can always efficiently and accurately fill the reagents.

[0063] In addition, the automated liquid filling system is also provided with a visual sensor. In the present embodiment, a first visual sensor 601 and a second visual sensor 602 are respectively provided in different areas. The visual sensor is used to monitor whether the transmission flow in the area is normal.

[0064] Please refer to Figure 6 , Figure 6 yes Figure 4Schematic diagram of the state of the filling unit in the automated liquid filling system when filling liquid. In the automated liquid filling system, a consumables adding part and a reagent adding part are arranged at the front end of the automated liquid filling mechanism, and the consumables adding part includes a plurality of stacked consumables receiving drawers, and the plurality of stacked consumables receiving drawers are located at the first span beam 10 near the consumables clamping unit 110, so as to facilitate the consumables transfer by the consumables clamping unit 110, and the plurality of stacked consumables receiving drawers include a linked tube amplification consumables receiving drawer unit 541, a deep well plate consumables receiving drawer unit 542, a first pipetting consumables receiving drawer unit 543, a second pipetting consumables receiving drawer unit 544, etc., which only illustrates the possible types of receiving positions of the consumables adding part and does not limit the specific layout position and quantity of each receiving drawer unit. The reagent adding part is an automated drawer unit that can be driven to move. The reagent adding part is arranged on a side close to the common guide rod 405. The reagent adding part includes a first reagent carrying position 551, a second reagent carrying position 552 and a magnetic bead reagent carrying position 553. A transmission channel 56 is arranged adjacent to the reagent drawer unit. The transmission channel 56 is located in the middle of the automated filling system. The transmission channel 56 is provided with a transmission channel interaction port 561. The transmission channel 56 can receive different types of consumables and pass them out of the automated liquid filling system through the transmission channel interaction port 561. The magnetic rod box storage position 53, the first type of pipetting consumables storage position 51 and the second type of pipetting consumables storage position 52 are all arranged close to the transmission channel 56, so that the transmission distance of each type of consumables is shorter and the system circulation efficiency is higher. Adjacent to the transmission channel 56, there are also arranged a pipetting operation area 57 and a magnetic bead oscillation area 58. The pipetting operation area 57 can be located near the position of the magnetic rod box storage position 53. The pipetting operation area 57 can receive the reagents in the first reagent carrying position 551 and / or the second reagent carrying position 552, and be opened and closed. The pipetting unit 220 can transfer the corresponding reagent to the corresponding hole position of the extraction consumables by suction. Here, the tubular clamping unit 210 can transfer the reagent bottle in the second preset position to the third preset position and open the cover. Here, the second preset position includes the first reagent carrying position 551 and / or the second reagent carrying position 552, and the third preset position is the pipetting operation area 57. An oscillation drive mechanism is arranged in the magnetic bead oscillation area 58, and the oscillation drive mechanism can perform a mixing operation on the magnetic bead liquid.

[0065] In this embodiment, in order to ensure that the pipetting has higher efficiency and minimize the differentiated requirements of different types of liquids for the pipetting volume and accuracy, two pipetting units 220 are provided, including a first pipetting unit located on the opposite side of the tubular clamping unit 210 on the second span beam 20 and a second pipetting unit located on the same side of the tubular clamping unit 210. The working steps of the automated liquid filling system provided in this embodiment are described below:

[0066] S10, the consumables clamping unit 110 of the first span beam 10 can be driven to transfer different types of consumables from multiple different types of consumables receiving positions of the consumables adding part to different operating positions, for example, transferring the amplification tube consumables to the transmission channel 56, transferring the first pipetting consumables to the first type of pipetting consumables storage position 51, etc.;

[0067] S20, the tubular clamping unit 210 of the second span beam 20 can clamp the reagent bottle in the reagent adding part to the pipetting operation area 57, and the tubular clamping unit 210 can cooperate with the reagent bottle clamping structure in the pipetting operation area 57 to perform the reagent bottle opening operation, and the first pipetting unit and / or the second pipetting unit can transfer the reagent in the reagent bottle and the magnetic bead liquid in the magnetic bead oscillation area 58 to the corresponding hole positions of the deep well plate consumables;

[0068] S30, the filling unit 310 performs the filling of the lysis reagent or the washing reagent, and then the extraction magnetic rod sleeve loading unit 120 takes the extraction magnetic rod sleeve from the magnetic rod sleeve box and transfers it to the corresponding hole position of the deep-well plate consumable to complete the preparation of the extraction system, and then the consumable clamping unit 110 transfers the deep-well plate consumable with the completed extraction system preparation to the transmission channel 56, and outputs it to perform subsequent operations. Of course, the two movable cross beams and the fixed cross beam can be driven to perform other operations to cooperate with the preparation of the extraction system in the deep-well plate consumable, which will not be repeated here.

[0069] The automated liquid filling system provided in this embodiment includes an automated liquid filling mechanism, a pipetting consumables storage position, an extraction magnetic rod sleeve storage position, a magnetic bead liquid oscillation position and a transmission channel. The first span beam and / or the second span beam can be driven to move between different operating positions to perform different operations. The third span beam is provided with a filling unit filling operating position and a cleaning tank spaced below it on the length direction side. The filling unit can be driven by the filling lifting drive mechanism to move to different operating positions to perform liquid filling or cleaning operations in the target consumables. The various operating positions in the automated liquid filling system can perform the construction and configuration of the extraction system in a highly integrated manner with the cooperation of two movable span beams and one fixed span beam.

[0070] The above contents are further detailed descriptions of the present invention in combination with specific preferred implementations, and the specific implementation of the present invention cannot be considered to be limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. An automated liquid filling mechanism, characterized in that: The invention comprises a common guide rail (403), a first span beam (10) and a second span beam (20) connected to the common guide rail (403), wherein the common guide rail (403) is provided with a first driving component and a second driving component, and the first driving component and the second driving component can respectively drive the first span beam (10) and the second span beam (20) to move on the common guide rail (403); the first span beam (10) is provided with a first span beam driving mechanism, and the two sides of the first span beam (10) are respectively provided with a connected consumable clamping unit and an extraction magnetic rod sleeve loading unit, and the first span beam driving mechanism can drive the consumable clamping unit and the extraction magnetic rod sleeve loading unit to move along the first span beam (10); the second span beam (20) is provided with a second span beam driving mechanism, and the second span beam (20) is provided with a second span beam driving mechanism. The two sides of the beam (20) are respectively provided with a tubular clamping unit and a pipetting unit connected to each other. The second span beam driving mechanism can drive the tubular clamping unit and the pipetting unit to move on the second span beam (20). The tubular clamping unit can clamp and transfer the reagent bottle and open and close the lid of the reagent bottle. It also includes a third span beam (30). The third span beam (30) is provided with a third span beam driving mechanism and a filling component (31). The third span beam driving mechanism can drive the filling component (31) to move on the third span beam (30). The filling component includes more than one filling unit. The filling unit is fluidically connected to a liquid consumable container to be filled, a fluid transmission channel and a liquid drive pump; it also includes a filling lifting driving mechanism that can adjust the vertical position of the filling unit.

2. The automatic liquid filling mechanism according to claim 1, characterized in that: The consumable clamping unit can clamp the consumable unit at the first preset position and transfer it as a consumable receiving unit, the tubular clamping unit transfers the reagent bottle at the second preset position to the third preset position and opens the cover, the pipetting unit transfers the reagent in the reagent bottle at the third preset position to the consumable receiving unit, the filling lifting drive mechanism drives the filling unit to the top of the consumable receiving unit, the liquid driving pump transfers the liquid to be filled in the liquid consumable container to be filled through the fluid transmission channel to the filling unit and then pumps it to the consumable receiving unit, and the extraction magnetic rod sleeve loading unit moves to the consumable receiving unit to complete the magnetic rod sleeve configuration.

3. The automatic liquid filling mechanism according to claim 1, characterized in that: It also includes a cleaning liquid consumable container and a cleaning tank. The filling lifting drive mechanism can drive the filling unit to the top of the cleaning tank. The liquid drive pump transfers the cleaning liquid in the cleaning liquid consumable container to the filling unit through the fluid transmission channel to complete the internal and external cleaning of the filling unit.

4. The automatic liquid filling mechanism according to claim 1, characterized in that: The second span beam driving mechanism comprises a second span beam driving motor, a second span beam driving wheel, a second span beam driven wheel, a second span beam transmission belt and a second span beam connecting block. The second span beam driving motor is arranged at the top of the second span beam. The output shaft of the second span beam driving motor is connected to the second span beam driving wheel. The second span beam driven wheel is arranged at a preset interval from the second span beam driving wheel. The second span beam driving belt is wound between the second span beam driving wheel and the second span beam driven wheel. A second span beam connecting block is connected to one side of the second span beam transmission belt. A second span beam sliding rail is arranged along the length direction of the second span beam (20). The second span beam connecting block is arranged with an engaging groove. The second span beam connecting block is slidably connected to the second span beam sliding rail.

5. The automatic liquid filling mechanism according to claim 1, characterized in that: The liquid receiving unit is also equipped with a liquid receiving assembly, which includes a liquid receiving plate driving motor, a liquid receiving driving gear, a liquid receiving transmission rack and a liquid receiving plate. The liquid receiving plate driving motor is connected to the liquid receiving driving gear, the liquid receiving driving gear is meshingly connected to the liquid receiving transmission rack, and the liquid receiving transmission rack is fixedly connected to the liquid receiving plate. When the liquid receiving plate driving motor rotates, the liquid receiving transmission rack can drive the liquid receiving plate to the bottom of the liquid receiving unit to receive dripping droplets.

6. The automatic liquid filling mechanism according to claim 1, characterized in that: The third span beam driving mechanism includes a third span beam driving motor, a third span beam driving wheel, a third span beam driven wheel, a third span beam transmission belt and a third span beam connecting block, wherein the output shaft of the third span beam driving motor is connected to the third span beam driving wheel, a third span beam driven wheel is arranged at a preset interval from the third span beam driving wheel, a third span beam transmission belt is wound between the third span beam driving wheel and the third span beam driven wheel, a third span beam connecting block is connected to one side of the third span beam transmission belt, and the third span beam connecting block is slidably connected to a third span beam slide rail extending in the length direction of the second span beam.

7. The automatic liquid filling mechanism according to claim 1, characterized in that: The configuration heights of the first span beam (10) and the second span beam (20) are higher than the configuration height of the third span beam (30), and the third span beam (30) is fixedly configured.

8. The automatic liquid filling mechanism according to claim 1, characterized in that: The extraction magnetic rod sleeve loading unit is provided with a loading drive motor, a loading transmission screw, a first loading connection block, a second loading connection block, a loading transmission slide rail and one or more magnetic rod sleeve capture units, wherein the loading drive motor can drive the loading transmission screw to rotate, so that the first loading connection block connected to the loading transmission screw moves along the length direction of the loading transmission screw, the loading transmission slide rail is arranged in the vertical direction of the extraction magnetic rod sleeve loading unit, the second loading connection block is engaged and connected to the loading transmission slide rail, and the second loading connection block is connected to the extraction magnetic rod sleeve loading unit.

9. The automatic liquid filling mechanism according to claim 8, characterized in that: The extraction magnetic rod sleeve loading unit is also provided with a separation structure and a separation drive motor. The separation structure includes a groove structure adapted to clamp the one or more magnetic rod sleeve acquisition units. The separation structure can be driven by the separation drive motor to insert into the combination position of the magnetic rod sleeve acquisition unit and the magnetic rod sleeve through lifting movement, and the separation structure can abut and separate the magnetic rod sleeve through the relative movement of lifting and lowering.

10. An automated liquid filling system, characterized in that: The automatic liquid filling mechanism according to claim 1 further comprises an extraction consumables carrying position, a pipetting consumables storage position, a magnetic rod box storage position, a transmission channel and a magnetic bead oscillation area, wherein: The extraction consumables carrying position, the pipetting consumables storage position and the magnetic rod sleeve box storage position are all located at the lower part of the first span beam (10) and the second span beam (20). The bottom of the extraction consumables carrying position is provided with an extraction consumables fitting groove, which can be slidably connected to the extraction consumables slide rail. The extraction consumables carrying position can be driven by the extraction consumables driving mechanism to slide along the extraction consumables slide rail. The extraction consumables driving mechanism includes an extraction consumables driving motor and a pulley transmission mechanism. The output of the extraction consumables driving motor is connected to the pulley transmission mechanism. The pulley transmission mechanism includes an extraction consumables driving motor and a pulley transmission mechanism. The transmission channel is provided with a transmission channel interaction port, and the magnetic rod box storage position, the first type of pipetting consumable storage position and the second type of pipetting consumable storage position are all arranged close to the transmission channel. The transmission channel can receive different types of consumables and transfer the consumables out of the automated liquid filling system through the transmission channel interaction port. An oscillation drive mechanism is arranged in the magnetic bead oscillation zone, and the oscillation drive mechanism can perform a mixing operation on the magnetic bead liquid.

Images