Auxiliary device for taking out eyeball retina specimen of laboratory mouse

Abstract

The utility model aims to provide an auxiliary device for taking out an eyeball retina specimen of an experimental mouse, which relates to the technical field of medical instruments and is characterized in that during use, a cornea of a taken-down eyeball of the mouse is placed below an operating cylinder upwards, and an arc-shaped sheet is controlled to clamp the cornea by rotating a cover plate of a reducing mechanism; mouse eyeballs of different sizes can be fixed through the diameter changing mechanism, in the moving process, the arc-shaped piece abuts against the blade to move towards the circle center of the base to be attached to the cornea, the mounting ring is rotated to cut the cornea, then the clamping mechanism slides downwards to be close to and clamp the cornea and the crystalline lens, and the cornea and the crystalline lens are separated. Then, the optic cup-shaped tissue with the cornea and the crystalline lens removed is reversed, the clamping rod continues to be used for fixing the optic nerve, the cup opening of the optic cup-shaped tissue abuts against the arc-shaped piece, then the clamping rod translates downwards, and the internal retina tissue is completely extruded out of the optic cup-shaped tissue through the innate subretinal space; therefore, an eyeball retina specimen is obtained.

Description

Technical Field

[0001] The utility model relates to the technical field related to medical devices, in particular to an auxiliary device for taking out retinal specimens of experimental mice eyes. Background Art

[0002] Mouse retina is a commonly used specimen in basic animal experimental research in ophthalmology, and can be used to detect protein expression and mRNA expression levels in the retina. In actual operation, it is often necessary to use a stereoscope or microscope to dissect the mouse eyeball, remove the anterior segment of the eye, and then separate the retina. The main structure of the eyeball includes the cornea in the front, the lens in the middle, the inner retina in the back, and the long optic nerve at the end. The retina itself has a subretinal cavity. Therefore, when taking the retina, this structure can be used to separate and squeeze the retina from the optic cup-shaped tissue.

[0003] Currently, the operation of obtaining mouse retina is relatively delicate and requires the assistance of ophthalmologists. It is difficult for novices or non-ophthalmologists to operate, and it is difficult to master it in a short time. It is inconvenient, the acquisition efficiency is low, and the removed retina is incomplete, which affects the progress and results of corresponding ophthalmic research experiments. Utility Model Content

[0004] In view of the deficiencies of the prior art, the present invention provides the following technical solutions:

[0005] An auxiliary device for taking out retinal specimens of experimental mice eyeballs comprises an operating cylinder, a mounting ring is rotatably arranged below the operating cylinder, a plurality of elastic telescopic rods are arranged in a circumferential array on the mounting ring, and a blade is fixedly installed at the front end of the elastic telescopic rods;

[0006] A diameter-changing mechanism is arranged below the mounting ring, and the diameter-changing mechanism comprises a base and a cover plate rotatably connected to the bottom surface of the mounting ring, the base is rotatably mounted below the cover plate, a plurality of arc-shaped pieces are stacked in a circular array on the base, the upper end of the blade is fixedly connected to the elastic telescopic rod, and the arc-shaped piece extends from the lower end, and the arc-shaped piece is controlled to move toward the center of the base by rotating the cover plate, and during the movement, the arc-shaped piece presses against the blade to move toward the center of the base;

[0007] A clamping mechanism is slidably arranged inside the operating cylinder, and the clamping mechanism includes a clamping rod and an outer cylinder slidably connected to the operating cylinder, the outer cylinder is sleeved outside the clamping rod, and two opened elastic clamping flaps are fixedly arranged at the front end of the clamping rod. By pulling up the clamping rod, the two elastic clamping flaps are pulled back into the outer cylinder, and the two elastic clamping flaps are gradually closed under the constraint of the outer cylinder.

[0008] Furthermore, the arc-shaped piece has a tough structure, a lower short column is fixedly arranged below one end of the arc-shaped piece, and an upper short column is fixedly arranged above one end, a mounting hole is provided on the base corresponding to the lower short column, and the arc-shaped piece is rotatably connected to the base through the cooperation between the lower short column and the mounting hole, a sliding groove is provided on the cover plate corresponding to the upper short column, and the upper short column is located in the sliding groove to slide and rotate, and by rotating the cover plate, the upper short column is driven to slide in the sliding groove, so that the arc-shaped piece moves around the axis of the lower short column toward the center of the base.

[0009] Furthermore, the blade extends obliquely outward from the center of the mounting ring into an arc-shaped piece, and the blade always presses against the arc-shaped piece under the action of the elastic telescopic rod.

[0010] Furthermore, an elastic element is sleeved on the outer wall of the outer cylinder of the clamping mechanism, and the upper end of the elastic element is connected to the rear end of the outer cylinder, and the lower end of the elastic element is connected to the operating cylinder.

[0011] Furthermore, an elastic element is disposed on the outer surface of the clamping rod of the clamping mechanism, and the upper end of the elastic element is connected to the tail end of the clamping rod, and the lower end of the elastic element is connected to the tail end of the outer tube, and the elastic clamping flap at the head end of the clamping rod is a tough structure.

[0012] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0013] 1. When the utility model is used, the cornea of ​​the removed mouse eyeball is placed upward under the operating cylinder, and the arc-shaped piece is controlled to move toward the center of the base to clamp the cornea by rotating the cover plate of the diameter-changing mechanism. During the movement, the arc-shaped piece presses against the blade and moves toward the center of the base to stick to the cornea, and the cornea is cut by rotating the mounting ring, and then the operating cylinder slides down to approach the cornea through the clamping mechanism slidably arranged on the operating cylinder, and the elastic clamping flap at the front end of the clamping rod is opened to contact the cornea and the lens by pushing down the clamping rod, and the elastic clamping flap is closed to clamp the cornea and the lens by pulling up the clamping rod, so that the cornea and the lens are separated from the optic cup-shaped tissue, and then the optic cup-shaped tissue with the cornea and the lens removed is reversed, and the clamping rod is continued to be used to fix the optic nerve, so that the cup mouth of the optic cup-shaped tissue presses against the arc-shaped piece, and then the clamping rod is translated downward to squeeze the internal retinal tissue completely out of the optic cup-shaped tissue by using the inherent subretinal cavity, so as to obtain the retinal specimen of the eyeball;

[0014] 2. The utility model can fix mouse eyeballs of different sizes through the diameter-changing mechanism, and clamp the eyeballs of different sizes through the arc plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the internal structure of the utility model;

[0016] Figure 2This is an enlarged schematic diagram of the internal structure A of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the diameter-changing mechanism of the utility model;

[0018] Figure 4 It is a top view schematic diagram of the diameter-changing mechanism of the utility model;

[0019] Figure 5 This is a schematic diagram of the arc plate structure of the utility model.

[0020] In the figure: reducing mechanism-1, base-101, mounting hole-1011, arc-shaped piece-102, upper short column-1021, lower short column-1022, cover plate-103, sliding groove-1031, mounting ring-2, elastic telescopic rod-3, blade-301, operating cylinder-4, clamping mechanism-5, clamping rod-501, elastic clamping flap-5011, outer cylinder-502. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Embodiment 1:

[0023] See also Figure 1-5 An auxiliary device for removing retinal specimens from experimental mouse eyes includes an operating cylinder 4, a mounting ring 2 is rotatably arranged below the operating cylinder 4, a plurality of elastic telescopic rods 3 are arranged in a circular array on the mounting ring 2, a blade 301 is fixedly installed at the front end of the elastic telescopic rod 3, the elastic telescopic rod 3 is a telescopic rod structure, an elastic element is arranged inside the elastic element, and the blade has a tendency to always move backwards through the pulling effect of the elastic element. By rotating the mounting ring 2, the blade 301 can be rotated to cut the cornea of ​​the eyeball.

[0024] Embodiment 2:

[0025] See also Figure 1-5According to Example 1, a diameter-changing mechanism 1 is provided below the mounting ring 2, and the diameter-changing mechanism 1 comprises a base 101 and a cover plate 103 rotatably connected to the bottom surface of the mounting ring 2, the base 101 is rotatably mounted below the cover plate 103, a plurality of arc-shaped pieces 102 are stacked in a circular array on the base 101, the upper end of the blade 301 is fixedly connected to the elastic telescopic rod 3, and the arc-shaped piece 102 extends from the lower end, and the arc-shaped piece 102 is controlled to move toward the center of the base 101 by rotating the cover plate 103, and during the movement, the arc-shaped piece 102 presses against the blade 301 to move toward the center of the base 101;

[0026] In this embodiment, the blade 301 extends obliquely outward from the center of the mounting ring 2 to form an arc-shaped piece 102. The blade 301 always presses against the arc-shaped piece 102 under the action of the elastic telescopic rod 3. When the eyeball is placed under the operating cylinder 4, the arc-shaped piece 102 is controlled to move toward the center of the base 101 to clamp the cornea by rotating the cover plate 103 of the diameter-changing mechanism 1. During the movement, the arc-shaped piece 102 presses against the blade 301 and moves toward the center of the base 101 to stick to the cornea, and the cornea is cut by rotating the mounting ring 2.

[0027] In this embodiment, the arc-shaped piece 102 has a tough structure, a lower short column 1022 is fixedly arranged below one end of the arc-shaped piece 102, and an upper short column 1021 is fixedly arranged above one end. A mounting hole 1011 is provided on the base 101 corresponding to the lower short column 1022. The arc-shaped piece 102 is rotatably connected to the base 101 through the cooperation between the lower short column 1022 and the mounting hole 1011. A sliding groove 1031 is provided on the cover plate 103 corresponding to the upper short column 1021. The upper short column 1021 is located in the sliding groove 1031 to slide and rotate. By rotating the cover plate 103, the upper short column 1021 is driven to slide in the sliding groove 1031, so that the arc-shaped piece 102 moves around the axis of the lower short column 1022 toward the center of the base 101.

[0028] Embodiment 3:

[0029] See also Figure 1-5 According to Example 1, a clamping mechanism 5 is slidably arranged in the operating cylinder 4, and the clamping mechanism 5 comprises a clamping rod 501 and an outer cylinder 502 slidably connected to the operating cylinder 4, the outer cylinder 502 is sleeved outside the clamping rod 501, and two opened elastic clamping petals 5011 are fixedly arranged at the front end of the clamping rod 501, and the two elastic clamping petals 5011 are pulled back into the outer cylinder 502 by pulling up the clamping rod 501, and the two elastic clamping petals 5011 are gradually closed under the constraint of the outer cylinder 502;

[0030] In this embodiment, the clamping mechanism 5 slidably arranged on the operating tube 4 slides down and approaches the cornea, and the clamping rod 501 is pushed down to open the elastic clamping flap 5011 at the front end of the clamping rod 501 to contact the cornea and the lens. The clamping rod 501 is pulled up to close the elastic clamping flap 5011 to clamp the cornea and the lens, thereby separating the cornea and the lens from the optic cup-shaped tissue. The optic cup-shaped tissue from which the cornea and the lens have been removed is then inverted, and the clamping rod 501 is continued to be used to fix the optic nerve so that the cup mouth of the optic cup-shaped tissue is against the arc-shaped piece 102. Then the clamping rod 501 is translated downward to squeeze the internal retinal tissue completely out of the optic cup-shaped tissue using the inherently existing subretinal cavity, thereby obtaining a retinal specimen of the eyeball.

[0031] Embodiment 4:

[0032] See also Figure 1-5 According to Example 3, an elastic element is sleeved on the outer wall of the outer cylinder 502 of the clamping mechanism 5, and the upper end of the elastic element is connected to the rear end of the outer cylinder 502, and the lower end of the elastic element is connected to the operating cylinder 4. An elastic element is sleeved on the clamping rod, and the upper end of the elastic element is connected to the rear end of the clamping rod 501, and the lower end of the elastic element is connected to the rear end of the outer cylinder 502. The elastic clamping flap 5011 at the head end of the clamping rod 501 is a tough structure. When the outer cylinder 502 is pushed down, the elastic element is compressed, and when the elastic element is released, the outer cylinder 502 slides up under the action of the elastic force. Similarly, when the clamping rod 501 is pushed down, the elastic element is compressed, and when it is released, the clamping rod 501 slides up under the action of the elastic force.

[0033] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. An auxiliary device for removing retinal specimens from experimental mice, comprising an operating tube, characterized in that: A mounting ring is rotatably arranged below the operating cylinder, and a plurality of elastic telescopic rods are arranged in a circular array on the mounting ring, and a blade is fixedly installed at the front end of the elastic telescopic rods; A diameter-changing mechanism is arranged below the mounting ring, and the diameter-changing mechanism comprises a base and a cover plate rotatably connected to the bottom surface of the mounting ring, the base is rotatably mounted below the cover plate, a plurality of arc-shaped pieces are stacked in a circular array on the base, the upper end of the blade is fixedly connected to the elastic telescopic rod, and the arc-shaped piece extends from the lower end, and the arc-shaped piece is controlled to move toward the center of the base by rotating the cover plate, and during the movement, the arc-shaped piece presses against the blade to move toward the center of the base; A clamping mechanism is slidably arranged inside the operating cylinder, and the clamping mechanism includes a clamping rod and an outer cylinder slidably connected to the operating cylinder, the outer cylinder is sleeved outside the clamping rod, and two opened elastic clamping flaps are fixedly arranged at the front end of the clamping rod. By pulling up the clamping rod, the two elastic clamping flaps are pulled back into the outer cylinder, and the two elastic clamping flaps are gradually closed under the constraint of the outer cylinder.

2. The auxiliary device for removing retinal specimens from experimental mice according to claim 1, characterized in that: The arc-shaped piece has a tough structure, a lower short column is fixedly arranged below one end of the arc-shaped piece, and an upper short column is fixedly arranged above one end. A mounting hole is arranged on the base corresponding to the lower short column, and the arc-shaped piece is rotatably connected to the base through the cooperation between the lower short column and the mounting hole. A sliding groove is arranged on the cover plate corresponding to the upper short column, and the upper short column slides and rotates in the sliding groove. By rotating the cover plate, the upper short column is driven to slide in the sliding groove, so that the arc-shaped piece moves around the axis of the lower short column toward the center of the base.

3. The auxiliary device for removing retinal specimens from experimental mice according to claim 1, characterized in that: The blade extends obliquely outward from the center of the mounting ring to form an arc-shaped piece, and the blade always presses against the arc-shaped piece under the action of the elastic telescopic rod.

4. The auxiliary device for removing retinal specimens from experimental mice according to claim 1, characterized in that: An elastic element is sleeved on the outer wall of the outer cylinder of the clamping mechanism, and the upper end of the elastic element is connected to the rear end of the outer cylinder, and the lower end of the elastic element is connected to the operating cylinder.

5. The auxiliary device for removing retinal specimens from experimental mice according to claim 1, characterized in that: An elastic element is disposed outside the clamping rod of the clamping mechanism, and the upper end of the elastic element is connected to the tail end of the clamping rod, and the lower end of the elastic element is connected to the tail end of the outer tube. The elastic clamping flap at the head end of the clamping rod is a tough structure.

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