Purification column supporting frame

Abstract

The utility model discloses a purification column supporting frame, and belongs to the field of protein purification. The purification column supporting frame comprises a frame, a fixed plate arranged in the frame in a sliding manner, a plurality of circular grooves formed in the fixed plate and distributed transversely and linearly, and a vertical plate arranged on the fixed plate, the clamping assemblies are arranged at the positions, corresponding to the upper portions of the circular grooves, of the fixing plate and used for clamping the purification columns, the adjusting assemblies are arranged on the vertical plates and used for adjusting the horizontal positions of the clamping assemblies, and a cooling assembly used for conducting cold storage protection on the purification columns is arranged at the bottom of the frame. The cooling assembly comprises a sealing box which is fixedly arranged at the bottom of the frame and is in sliding connection with the fixing plate, the purification column supporting frame achieves cooling of the purification column through the cooling assembly, and the problem that the purification column is prone to losing activity due to high temperature is avoided.

Description

Technical Field

[0001] The utility model relates to the field of protein purification, in particular to a purification column support frame. Background Art

[0002] Protein separation and purification is an important operating technique widely used in biochemical research applications. A typical eukaryotic cell can contain thousands of different proteins, some of which are very abundant, while others contain only a few copies. In order to study a certain protein, it must first be purified from other proteins and non-protein molecules. The essential experimental equipment in the process of protein separation and purification is the purification column.

[0003] A Chinese patent with publication number CN219220934U discloses a protein purification column support frame. The device provides a load-bearing groove, utilizes the elastic adjustment effect of a first clamping plate, cooperates with a second clamping plate, and then utilizes an arc-shaped clamping plate to clamp and stabilize the protein purification column, thereby improving the convenience and stability of the protein purification column during placement and subsequent removal. However, the existing device is difficult to ensure the quality of the purification column. During use, it is found that the protein attached to the column can easily lose its activity due to excessively high temperature, thereby causing the failure of the experiment. Once the experimental process is too long, it is easy to cause the protein to denature and fail.

[0004] Therefore, the utility model provides a purification column support frame to solve the above problems. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] The utility model provides a purification column support frame, aiming to solve the problem that the existing protein purification column support frame mentioned in the background technology is difficult to ensure the quality of the purification column. During use, it is found that the protein attached to the column can easily lose its activity due to excessively high temperature, thereby causing the failure of the experiment. Once the experimental process is too long, it is easy to cause the protein to denature and fail.

[0007] (II) Technical solution

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a purification column support frame, comprising a frame, a fixed plate slidably arranged inside the frame, a plurality of circular grooves arranged on the fixed plate and distributed in a transverse linear manner, a vertical plate arranged on the fixed plate, a clamping assembly arranged on the fixed plate at a position above the corresponding plurality of circular grooves for clamping the purification column, and an adjusting assembly arranged on the vertical plate for adjusting the horizontal position of the clamping assembly; when in use, the purification column to be stored is first placed in the circular groove on the fixed plate inside the frame, a stable clamping effect is achieved by using the clamping assembly, and elastic clamping and fixing is achieved by using the adjusting assembly, so that it is easy to adapt to and clamp purification columns of different diameters.

[0009] In order to prevent the protein attached to the column from losing its activity due to excessively high temperature, thereby causing the failure of the experiment; a cooling component for refrigerating and protecting the purification column is arranged at the bottom of the frame, and the cooling component includes a sealing box fixedly arranged at the bottom of the frame and slidably connected to the fixed plate, a buffer part fixedly arranged at the top of the sealing box and in contact with the bottom of the purification column, a notch opened on one side of the sealing box, a placement box for containing ice cubes slidably arranged inside the notch and located at the bottom of the buffer part, a lower water tank opened at the bottom of the placement box, a receiving groove opened at a position below the sealing box corresponding to the placement box, a water collecting tank fixedly arranged at a position on one side of the frame, and a water collecting tank opened at the bottom of the frame. The rack corresponds to a position on one side of the water collecting tank and is connected to the accommodating tank and the water collecting tank, and a fixing component is arranged on the side of the sealed box away from the notch for fixing the placement box; the low-temperature storage of the purification column can be achieved by utilizing the cooling component, before use, the placement box inside the sealed box is first pulled out along the handle on the U-shaped card board, ice cubes are stored inside the placement box, and then pushed into the sealed box, so as to achieve the cooling effect later, when the ice cubes melt, in order to avoid water overflow, a lower water tank can be set up to cooperate with the drainage trough, and the melted water is discharged into the interior of the water collecting tank for unified treatment, wherein the buffer part is a rubber sponge with an elastic buffering effect to avoid the impact of accidentally touching the purification column from the top.

[0010] Preferably, in order to achieve the fixation of the placement box; the fixing component includes a rectangular slot opened on the side of the sealing box away from the notch, a rectangular block fixedly arranged on the side of the placement box away from the notch and slidably engaged with the rectangular slot, and a fixed block rotatably arranged on the side of the rectangular block away from the placement box, the fixed block and the rectangular block are consistent in size; the fixing component can be used to limit and fix it, and when the placement box is pushed in, the fixed block is first rotated to a horizontal position so that it coincides with the position of the rectangular block. At this time, it is pushed in along the notch so that the rectangular block is engaged in the rectangular slot, the fixed block passes through the rectangular slot, and the U-shaped card board is engaged between the sealing box and the frame. At this time, the fixed block is rotated to a vertical direction. At this time, when the placement box is pulled outward, the fixed block achieves a limiting effect, thereby completing the fixation of the entire placement box.

[0011] Preferably, in order to achieve reasonable limited extraction of the U-shaped card plate; a U-shaped card plate is fixedly connected to one side of the placement box close to the groove, the raised end of the U-shaped card plate is slidably clamped between the sealing box and the frame, and a handle is fixedly provided on the U-shaped card plate; this can be achieved by setting a handle.

[0012] Preferably, in order to achieve reasonable clamping; the clamping assembly includes a first clamping plate and a second clamping plate symmetrically arranged on the frame at positions on both sides of the plurality of circular grooves, and a first arc-shaped clamping plate and a second arc-shaped clamping plate respectively fixedly arranged on one side of the first clamping plate and the second clamping plate close to each other and located at positions corresponding to the circular grooves; the first clamping plate is fixedly connected to the frame, and the second clamping plate is slidably connected to the frame; the position of the second clamping plate can be adjusted to drive the position of the second arc-shaped clamping plate, so that the purification column is clamped between the first arc-shaped clamping plate and the second arc-shaped clamping plate to achieve a fixing effect.

[0013] Preferably, in order to achieve reasonable adjustment of the position of the second clamping plate and to facilitate the fixed clamping of the purification column; the adjustment component includes a support rod that transversely passes through the vertical plate and is fixedly connected to the second clamping plate, a pull plate fixedly arranged on the support rod at one end away from the vertical plate, and a tension spring that is sleeved on the support rod and whose two ends are respectively fixedly connected to the pull plate and the vertical plate, and the support rod is slidably connected to the vertical plate; it can be adjusted by utilizing the adjustment component, wherein the tension spring is rectangular and elastic, and can automatically adjust the clamping according to the diameter of the purification column.

[0014] Preferably, in order to prevent the adjusting component from protruding outside and being easily squeezed by external force, the adjusting component also includes a protective box fixedly arranged on the side of the vertical plate away from the second clamping plate and located on the outside of the support rod, a limiting groove opened on the side of the protective box away from the vertical plate, and a limiting rod fixedly arranged on the pull plate and slidably engaged with the limiting groove; protection can be performed by utilizing a protective box, wherein the limiting rod is engaged with the limiting groove, thereby achieving the effect of stabilizing the fiber.

[0015] (III) Beneficial effects

[0016] The purification column support frame cools the purification column by providing a cooling component to avoid the problem of the purification column losing its activity due to high temperature. At the same time, a fixing component is provided to achieve reasonable positioning of the placement box for placing the diseased condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of a purification column support frame;

[0018] Figure 2 It is a schematic diagram of the unfolded structure of a purification column support frame;

[0019] Figure 3 It is a schematic diagram of the partial structure of a purification column support frame;

[0020] Figure 4 The figure is a schematic diagram of the cross-sectional structure of a purification column support frame.

[0021] In the figure:

[0022] 1. Frame; 2. Fixed plate; 21. Round groove; 22. Vertical plate;

[0023] 3. Clamping assembly; 31. First clamping plate; 311. First arc-shaped clamping plate; 32. Second clamping plate; 321. Second arc-shaped clamping plate;

[0024] 4. Adjustment assembly; 41. Support rod; 42. Extension spring; 43. Pull plate; 44. Protective box; 45. Limiting groove; 46. Limiting rod

[0025] 5. Cooling assembly; 51. Sealing box; 52. Buffering part;

[0026] 53. Placement box; 531. Sink;

[0027] 54. Notch;

[0028] 55. U-shaped pallet; 551. handle;

[0029] 56, fixed component; 561, rectangular slot; 562, rectangular card block; 563, fixed card block;

[0030] 57. Water collecting tank; 571. Drainage trough. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] Example 1

[0033] This embodiment provides a purification column support frame, such as Figure 1-4 As shown, the purification column support frame includes a frame 1, a fixed plate 2 slidably arranged inside the frame 1, a plurality of circular grooves 21 arranged on the fixed plate 2 and distributed in a transverse linear manner, a vertical plate 22 arranged on the fixed plate 2, a clamping assembly 3 arranged on the fixed plate 2 at a position above the plurality of circular grooves 21 for clamping the purification column, and an adjusting assembly 4 arranged on the vertical plate 22 for adjusting the horizontal position of the clamping assembly 3. A cooling assembly 5 for refrigerating and protecting the purification column is arranged at the bottom of the frame 1. The cooling assembly 5 includes a sealing box 51 fixedly arranged at the bottom of the frame 1 and slidably connected to the fixed plate 2, and a sealing box 51 fixedly arranged at the sealing box 5 1 and in contact with the bottom of the purification column, a buffer portion 52 disposed at the top of the sealing box 51 and in contact with the bottom of the purification column, a notch 54 provided on one side of the sealing box 51, a placement box 53 slidably disposed inside the notch 54 and located at the bottom of the buffer portion 52 for containing ice cubes, a lower water tank 531 provided at the bottom of the placement box 53, a receiving tank provided at a position below the sealing box 51 corresponding to the placement box 53, a water collecting box 57 fixedly provided at a position on one side of the frame 1, a drainage tank 571 provided at a position on one side of the frame 1 corresponding to the water collecting box 57 and communicating with the receiving tank and the water collecting box 57, and a fixing component 56 provided at a side of the sealing box 51 away from the notch 54 and used for fixing the placement box 53.

[0034] When in use, the purification column to be stored is first placed in the circular groove 21 on the fixed plate 2 inside the frame 1, and the clamping component 3 is used to achieve a stable clamping effect. The adjustment component 4 is used to achieve elastic clamping and fixing, which is convenient for adapting to clamp purification columns of different diameters. After the clamping is completed, in order to avoid the protein attached to the column from easily losing its activity due to excessively high temperature, thereby causing the failure of the experiment, once the experimental process is too long, it is easy to cause protein denaturation and failure, the purification column can be stored at low temperature by using the cooling component 5. Before use, the placement box 53 inside the sealed box 51 is first pulled out along the handle 551 on the U-shaped card plate 55, ice cubes are stored inside the placement box 53, and then pushed into the sealed box 51, so as to achieve the cooling effect later. When the ice cubes melt, in order to avoid water overflow, a lower water tank 531 can be set to cooperate with the drainage tank 571, and the melted water is discharged into the water collecting box 57 for unified treatment. The buffer part 52 is a rubber sponge with an elastic buffering effect to avoid the impact caused by accidentally touching the purification column from the top.

[0035] Specifically, the fixing assembly 56 includes a rectangular slot 561 provided on the side of the sealing box 51 away from the notch 54, a rectangular block 562 fixedly arranged on the side of the placement box 53 away from the notch 54 and slidably engaged with the rectangular slot 561, and a fixed block 563 rotatably arranged on the side of the rectangular block 562 away from the placement box 53. The fixed block 563 is consistent in size with the rectangular block 562. A U-shaped card plate 55 is fixedly connected to the side of the placement box 53 close to the notch 54. The raised end of the U-shaped card plate 55 is slidably engaged between the sealing box 51 and the frame 1. A handle 551 is fixedly arranged on the U-shaped card plate 55. When the placement box 53 is extracted and stored, In order to fix the placement box 53, it can be limited and fixed by using the fixing component 56. When pushing the placement box 53, first rotate the fixing block 563 to a horizontal position so that it coincides with the position of the rectangular block 562. At this time, push it in along the notch 54 so that the rectangular block 562 is engaged in the rectangular slot 561. The fixing block 563 passes through the rectangular slot 561, and the U-shaped card plate 55 is engaged between the sealing box 51 and the frame 1. At this time, rotate the fixing block 563 to a vertical direction. At this time, when the placement box 53 is pulled outward, the fixing block 563 achieves a limiting effect, thereby completing the fixation of the entire placement box 53.

[0036] Among them, in order to achieve reasonable clamping; the clamping assembly 3 includes a first clamping plate 31 and a second clamping plate 32 symmetrically arranged at the positions on both sides of the frame 1 corresponding to multiple circular grooves 21, and a first arc clamping plate 311 and a second arc clamping plate 321 respectively fixedly arranged on one side of the first clamping plate 31 and the second clamping plate 32 close to each other and located at the positions of the corresponding circular grooves 21. The first clamping plate 31 is fixedly connected to the frame 1, and the second clamping plate 32 is slidably connected to the frame 1; the position of the second clamping plate 32 can be adjusted to drive the position of the second arc clamping plate 321, so that the purification column is clamped between the first arc clamping plate 311 and the second arc clamping plate 321 to achieve a fixing effect.

[0037] Furthermore, the adjusting component 4 includes a support rod 41 that passes through the vertical plate 22 horizontally and is fixedly connected to the second clamping plate 32, a pulling plate 43 fixedly arranged at one end of the support rod 41 away from the vertical plate 22, and a tension spring 42 that is sleeved on the support rod 41 and whose two ends are respectively fixedly connected to the pulling plate 43 and the vertical plate 22. The support rod 41 is slidably connected to the vertical plate 22. In order to achieve a reasonable adjustment of the position of the second clamping plate 32 and to facilitate the fixed clamping of the purification column, the adjustment can be performed by utilizing the adjusting component 4, wherein the tension spring 42 is rectangular and elastic and can automatically adjust the clamping according to the diameter of the purification column.

[0038] Example 2

[0039] What is different from Example 1 is that the purification column support frame also includes an adjustment component 4, a protective box 44 fixedly arranged on the side of the vertical plate 22 away from the second clamping plate 32 and located on the outside of the support rod 41, a limiting groove 45 opened on the side of the protective box 44 away from the vertical plate 22, and a limiting rod 46 fixedly arranged on the pull plate 43 and slidably engaged with the limiting groove 45; in order to prevent the adjustment component 4 from protruding outside and being easily squeezed by external force, thereby causing damage to the purification column, protection can be provided by utilizing the protective box 44, wherein the limiting rod 46 is engaged with the limiting groove 45, thereby achieving the effect of stabilizing the fiber.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the field of technology within the technical scope disclosed by the present invention can make equivalent substitutions or changes based on the technical scheme and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A purification column support frame, comprising a frame (1), a fixed plate (2) slidably arranged inside the frame (1), a plurality of circular grooves (21) arranged on the fixed plate (2) and distributed in a transverse linear manner, a vertical plate (22) arranged on the fixed plate (2), a clamping assembly (3) arranged on the fixed plate (2) at a position above the plurality of circular grooves (21) for clamping the purification column, and an adjusting assembly (4) arranged on the vertical plate (22) for adjusting the horizontal position of the clamping assembly (3); Features: A cooling assembly (5) for refrigerating and protecting the purification column is provided at the bottom of the frame (1); The cooling assembly (5) comprises a sealing box (51) fixedly arranged at the bottom of the frame (1) and slidably connected to the fixing plate (2), a buffer portion (52) fixedly arranged at the top of the sealing box (51) and in contact with the bottom of the purification column, a notch (54) opened on one side of the sealing box (51), a placement box (53) slidably arranged inside the notch (54) and located at the bottom of the buffer portion (52) for containing ice cubes, a lower water tank (531) opened at the bottom of the placement box (53), a receiving tank opened at a position below the sealing box (51) corresponding to the placement box (53), a water collecting box (57) fixedly arranged at a position on one side of the frame (1), a drainage tank (571) opened at a position on one side of the frame (1) corresponding to the water collecting box (57) and connected to the receiving tank and the water collecting box (57), and a fixing assembly (56) arranged on a side of the sealing box (51) away from the notch (54) for fixing the placement box (53).

2. The purification column support according to claim 1, characterized in that: The fixing assembly (56) comprises a rectangular slot (561) provided on a side of the sealing box (51) away from the notch (54), a rectangular block (562) fixedly arranged on a side of the placement box (53) away from the notch (54) and slidably engaged with the rectangular slot (561), and a fixed block (563) rotatably arranged on a side of the rectangular block (562) away from the placement box (53), wherein the fixed block (563) is of the same size as the rectangular block (562).

3. The purification column support according to claim 2, characterized in that: A U-shaped cardboard (55) is fixedly connected to one side of the placement box (53) close to the notch (54); the raised end of the U-shaped cardboard (55) is slidably clamped between the sealing box (51) and the frame (1); and a handle (551) is fixedly provided on the U-shaped cardboard (55).

4. The purification column support according to claim 1, characterized in that: The clamping assembly (3) comprises a first clamping plate (31) and a second clamping plate (32) symmetrically arranged on the frame (1) at positions on both sides corresponding to the plurality of circular grooves (21), and a first arc-shaped clamping plate (311) and a second arc-shaped clamping plate (321) respectively fixedly arranged on one side of the first clamping plate (31) and the second clamping plate (32) close to each other and located at positions corresponding to the circular grooves (21); the first clamping plate (31) is fixedly connected to the frame (1), and the second clamping plate (32) is slidably connected to the frame (1).

5. The purification column support according to claim 4, characterized in that: The adjustment assembly (4) comprises a support rod (41) which runs transversely through the vertical plate (22) and is fixedly connected to the second clamping plate (32), a pull plate (43) which is fixedly arranged at one end of the support rod (41) away from the vertical plate (22), and a tension spring (42) which is sleeved on the support rod (41) and has two ends respectively fixedly connected to the pull plate (43) and the vertical plate (22); the support rod (41) is slidably connected to the vertical plate (22).

6. The purification column support according to claim 5, characterized in that: The adjustment assembly (4) further comprises a protection box (44) fixedly arranged on a side of the vertical plate (22) away from the second clamping plate (32) and located outside the support rod (41), a limiting groove (45) provided on a side of the protection box (44) away from the vertical plate (22), and a limiting rod (46) fixedly arranged on the pull plate (43) and slidably engaged with the limiting groove (45).

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