Culture dish for molecular biology
By setting up an injection and suction system at the bottom of the molecular biology culture dish, supplementing nutrient solution and draining water droplets without opening the lid, the problems of bacterial contamination and water droplet condensation in the existing technology are solved, and the accuracy and sterility of the experimental results are improved. Guarantee of conditions.
Patent Information
- Application Number
- CN202421162061.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-27
- Publication Date
- 2025-02-18
- Estimated Expiration
- 2034-05-27
AI Technical Summary
The existing molecular biology petri dishes require multiple open cover operations in the experiment, resulting in bacterial contamination and inaccurate experimental results. At the same time, the water droplets are difficult to eliminate after high-temperature sterilization, which affects sterile conditions.
Design a molecular biology petri dish, and set up an injection and suction system through the bottom opening of the dish, including a transparent cover and a suction cylinder. There is an injection and suction hole opened on the side wall of the transparent cover, so as to supplement nutrient solution and drain water droplets without opening the cover, and reduce bacteria pollute.
Air pollution is reduced through the injection and suction system without opening the lid, the accuracy of the experimental results is improved, and the water droplets are discharged through the injection and suction holes, simplifying the disinfection process and ensuring sterile conditions.
Smart Images
Figure CN222499137U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of biological culture equipment, in particular to a molecular biology culture dish. Background Art
[0002] Molecular biology is the science that studies life phenomena at the molecular level. It explains the essence of various life phenomena by studying the structure, function and biosynthesis of biological macromolecules (nucleic acids, proteins). The research content includes various life processes, such as photosynthesis, molecular mechanisms of development, mechanisms of neural activity, cancer occurrence, etc. Molecular biology studies the structure and function of biological macromolecules at the molecular level to explain the essence of life phenomena. Since the 1950s, molecular biology has been the forefront and growth point of biology. Its main research areas include protein systems, protein-nucleic acid systems (centered on molecular genetics) and protein-lipid systems (i.e. biological membranes). The study of biological macromolecules, especially the structure and function of proteins and nucleic acids, is the foundation of molecular biology. The application of modern chemical and physical theories, technologies and methods has promoted the study of the structure and function of biological macromolecules, resulting in the vigorous development of molecular biology in the past 30 years. In molecular biology, culture dishes are indispensable supplies.
[0003] Among the existing conventional molecular biology culture dishes, there are usually only simple culture dishes. In most biological experiments, it is necessary to inject nutrient solution multiple times. Conventional culture dishes need to be opened multiple times. Since opening the lid will inevitably expose the test object to air, and there are bacteria in the air, there is a problem that the test object is contaminated and the experimental results are inaccurate. In addition, when high-temperature sterilization is used for disinfection of the culture dish, it usually causes water vapor to condense on the top of the dish and drip and gather at the bottom of the dish. It is difficult to ensure the sterile conditions of the culture by wiping with foreign objects, and natural air drying is time-consuming and laborious. Therefore, it is necessary to propose a molecular biology culture dish that can reduce bacterial contamination when opening the lid and facilitate disinfection. Utility Model Content
[0004] In order to solve the problem of bacterial contamination caused by opening the dish cover in the above-mentioned experiment and cleaning the culture dish after the experiment, the purpose of the utility model is to provide a molecular biology culture dish, in which an injection and suction system is arranged through the opening at the bottom of the dish, so that nutrient solution can be replenished without opening the cover during the experiment and water droplets can be conveniently discharged during cleaning, thereby achieving the effect of reducing bacterial contamination.
[0005] In order to achieve the above-mentioned purpose, the technical scheme of the utility model is as follows: a molecular biology culture dish, comprising a culture dish, an opening is arranged at the bottom of the culture dish, the culture dish is slidably connected to an injection and suction system through the opening, the injection and suction system comprises a transparent cover and an injection and suction tube, the transparent cover is fixedly connected to the injection and suction tube, the transparent cover is located at the opening at the bottom of the culture dish, the injection and suction tube is located at the lower part of the transparent cover, a plurality of injection and suction holes are opened on the side wall of the transparent cover, the injection and suction tube comprises a sealed sleeve and a cylinder body, the sealed sleeve is located at the upper part of the cylinder body and the lower part of the transparent cover, the sealed sleeve and the cylinder body are fixedly connected, an injection and suction tube is passed through the inside of the cylinder body, the injection and suction tube comprises a plurality of first injection and suction tubes and a second injection and suction tubes, one end of the plurality of first injection and suction tubes respectively corresponds to the injection and suction holes, and the other end of the first injection and suction tubes is connected to the second injection and suction tubes.
[0006] The principle of the basic scheme is: when the condition liquid needs to be replenished once or multiple times in the experiment, first pull the folding pull rod, the folding pull rod drives the injection and suction tube to extend and fix the injection and suction tube, and then push the injection and suction tube to raise the slidably connected transparent cover to a certain height, at this time the injection and suction hole on the side wall of the transparent cover is exposed, and then the liquid to be replenished is injected into the second injection pipette to realize the replenishment of the condition liquid without opening the cover;
[0007] When water droplets accumulate in the culture dish after cleaning and sterilization, the injection and suction holes are exposed in the same manner as above, and the water droplets in the culture dish are sucked out by suction.
[0008] The beneficial effects are: 1. The injection and suction system is slidably connected to the bottom of the culture dish, and since there is no need to open the cover to replenish the conditional liquid, the problem of air pollution affecting the experimental results is reduced.
[0009] 2. The water droplets accumulated in the culture dish can be discharged through the injection and suction holes to save time and cost. At the same time, the discharge of water droplets can reduce the problem of bacteria in the water contaminating the experimental objects and improve the accuracy of the experiment.
[0010] Furthermore, the culture dish comprises a dish body and a dish cover, and the dish body and the dish cover are threadedly connected.
[0011] The beneficial effect of the basic scheme is that when the biological experiment is carried out, the threads of the culture dish and the dish cover are tightened to ensure tightness. The design of the threaded connection between the culture dish and the dish cover ensures the airtightness of the experimental object in the experiment and reduces the problem of bacterial contamination caused by air contact with the experimental object.
[0012] Furthermore, a first rubber sleeve is detachably connected to the bottom of the dish body, and the first rubber sleeve is provided with a through opening corresponding to the bottom of the dish body.
[0013] The beneficial effect of the basic scheme is that when replenishing the conditional liquid, the first rubber sleeve is covered on the bottom of the culture medium dish. Since the culture dish is fragile and easy to break, the rubber sleeve covering ensures its safety, reduces the problem of the culture dish being damaged due to improper operation when replenishing the liquid, and also reduces the problem of contamination affecting the experimental results.
[0014] Furthermore, a plurality of support rods are arranged on four directions of the first rubber sleeve, and the support rods can be stored in the first rubber sleeve.
[0015] The beneficial effect of the basic solution is that by buckling out the retractable support rod, the culture dish can be placed horizontally and stably on the table, and at the same time, a certain space is given to the injection pipette to inject conditioned liquid and absorb water droplets, thereby achieving the effect of convenient liquid replenishment.
[0016] Furthermore, a second rubber sleeve is arranged on the top of the dish cover, and both the first rubber sleeve and the second rubber sleeve are made of elastic material.
[0017] The beneficial effect of the basic solution is that when storing the culture dish, the second rubber sleeve covers the dish cover, and both the first rubber sleeve and the second rubber sleeve are elastic. This design further ensures the safety of the culture dish and reduces the problem of culture dish loss when storing unused culture dishes.
[0018] Furthermore, the inner bottom of the dish body is provided with a slope of a middle bottom and a height around the middle bottom which diverges to the surrounding areas with the opening as the center.
[0019] The beneficial effect of the basic solution is that when the culture dish is sterilized, water droplets may fall from the top of the dish. The slope design allows the water droplets to naturally gather towards the transparent cover at the bottom of the slope according to inertia, thereby improving the efficiency of water droplet discharge and reducing the problem of bacteria in the water contaminating the experimental objects.
[0020] Furthermore, the cylinder body comprises a first telescopic cylinder and a second telescopic cylinder. The first telescopic cylinder is slidably connected to the second telescopic cylinder inside, and a folding pull rod is fixedly connected to the bottom of the second telescopic cylinder.
[0021] The beneficial effect of the basic solution is: when pulling out the cylinder, the folding pull rod will transmit the pulling force to pull out the first telescopic cylinder. The design of the first telescopic cylinder is thick at the top and thin at the bottom. The first telescopic cylinder will drive the second telescopic cylinder to be pulled out. The design of the first telescopic cylinder and the second telescopic cylinder allows the cylinder to be retracted to the bottom of the culture dish, so that the injection and suction system and the culture dish become a whole, that is, it does not affect the flatness of the culture dish, nor does it cause other influences on the experiment, reducing the problem of the experimental results being affected by the unevenness of the culture dish.
[0022] Furthermore, a spring clamping column is provided on the outer wall of the cylinder, and a clamping groove corresponding to the clamping head is provided on the inner wall of the bottom opening of the dish body opposite to the cylinder body. The spring clamping column includes a clamping head, a pressing head and a spring. The clamping head is located at the upper part of the spring clamping column. The clamping head and the pressing head are fixedly connected by a rod, and the spring is located at the bottom of the clamping head.
[0023] The beneficial effect of the basic solution is that when the transparent cover rises to the position where the injection and suction holes are exposed, the clamping head located on the side wall of the injection and suction cylinder will just reach the clamping slot, and the clamping head will be ejected by the spring at its bottom end. The clamping head will bounce into the clamping slot to achieve the effect of a fixed rising state, thereby reducing the problem of damaging the culture dish due to operational errors. As the clamping head pops up, the pressing head will be driven to pop up together through the connecting rod. When the transparent cover needs to be lowered after liquid replenishment is completed, pressing the pressing head will drive the clamping head to shrink together, achieving the effect of sliding down, which facilitates the operation when replenishing liquid. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 This is an isometric view of the disassembled components of the molecular biology culture dish in the embodiment of the present utility model.
[0025] Figure 2 It is a front cross-sectional view of the molecular biology culture dish body in the embodiment of the utility model.
[0026] Figure 3 It is an axonometric view of the injection tube in the molecular biology culture dish in the embodiment of the present utility model.
[0027] Figure 4 It is a front cross-sectional view of the injection and aspiration cylinder in the molecular biology culture dish in the embodiment of the utility model. DETAILED DESCRIPTION
[0028] The following is further described in detail through specific implementation methods:
[0029] The figure marks in the drawings of the specification include: dish body 1, first rubber sleeve 2, transparent cover 3, injection and suction hole 4, clamping groove 5, sealing sleeve 6, injection and suction cylinder 7, first telescopic cylinder 8, second telescopic cylinder 9, pressing head 10, clamping head 11, spring 12, folding pull rod 13, first injection and suction tube 14, dish cover 15, second rubber sleeve 16, second injection and suction tube 17.
[0030] Embodiment 1, basically as attached Figure 1-Figure 2 As shown: a molecular biology culture dish, comprising a culture dish, the culture dish comprising a dish body 1 and a dish cover 15, the dish body 1 and the dish cover 15 are threadedly connected, an opening is provided at the bottom of the dish body 1, and the dish body 1 is slidably connected to an injection and suction system through the opening;
[0031] The injection and suction system includes a transparent cover 3 and an injection and suction cylinder 7, the transparent cover 3 is fixedly connected to the injection and suction cylinder 7, the transparent cover 3 is located at the bottom opening of the dish body, the injection and suction cylinder 7 is located at the lower part of the transparent cover 3, and a plurality of injection and suction holes 4 are opened on the side wall of the transparent cover 3;
[0032] The injection and suction cylinder 7 includes a sealed sleeve 6 and a cylinder body. The sealed sleeve 6 is located at the lower part of the transparent cover 3. The sealed sleeve 6 and the cylinder body are fixedly connected. The cylinder body includes a first telescopic cylinder 8 and a second telescopic cylinder 9. The first telescopic cylinder 8 is slidably connected to the second telescopic cylinder 9. A folding pull rod 13 is fixedly connected to the bottom of the second telescopic cylinder 9. An injection and suction pipe is arranged through the cylinder body. The injection and suction pipe includes a plurality of first injection and suction pipes 14 and a second injection and suction pipes 17. The plurality of first injection and suction pipes 14 correspond to the injection and suction holes 4 respectively. The first injection and suction pipes 14 are connected to the second injection and suction pipes 17 at one end away from the injection and suction holes 4. A spring clamping column is provided on the outer wall of the cylinder body. The spring clamping column includes a clamping head 11, a pressing head 10 and a spring 12. The clamping head 11 is located at the upper part of the spring clamping column. The clamping head 11 and the pressing head 10 are fixedly connected by a rod. The spring 12 is located at the bottom of the clamping head 11. A clamping groove 5 corresponding to the clamping head 11 is provided on the inner wall of the bottom opening of the dish body 1 relative to the injection and suction cylinder 7.
[0033] The specific implementation process is as follows:
[0034] At the beginning of the biological experiment, the experimental object and the environmental liquid are injected into the dish body 1, and then the dish body 1 and the dish cover 15 are screwed tightly to ensure tightness. When the conditional liquid needs to be supplemented once or multiple times during the experiment, the first rubber sleeve 2 is first covered on the bottom of the dish body 1, and then the several retractable support rods are buckled out, the culture dish is placed flat on the laboratory table, and then the folding pull rod 13 is pulled, and the folding pull rod 13 will transmit the pulling force to pull out the first telescopic cylinder 8. The design of the first telescopic cylinder 8 is thick at the top and thin at the bottom. The first telescopic cylinder 8 will drive the second telescopic cylinder 9 to be pulled out until the first telescopic cylinder 8 and the second telescopic cylinder 9 are completely pulled out, and then the injection and suction cylinder 7 is pushed to raise the slidably connected transparent cover 3 to a certain height. At this time, the injection and suction holes 4 on the side wall of the transparent cover 3 are exposed, and the position The clamping head 11 on the side wall of the injection and suction cylinder 7 will just reach the card slot 5, and the clamping head 11 will be popped out by the spring 12 at its bottom end. The clamping head 11 will bounce into the card slot 5 to achieve the effect of a fixed ascending state. As the clamping head 11 pops out, the pressing head 10 will be driven to pop out together through the connecting rod, and then the liquid to be replenished will be injected into the second injection and suction tube 17. The liquid to be replenished will flow out from the injection and suction hole 4 through the first injection and suction tube 14 to achieve the conditional liquid replenishment without opening the cover. After the liquid is replenished, the clamping head 11 will pop out together with the pressing head 10 through the connecting rod. At this time, pressing the pressing head 10 drives the clamping head 11 to retract back into the cylinder body. At this time, the entire injection and suction system completes the liquid replenishment and descends to its original position.
[0035] Example 2
[0036] The difference from the above embodiment is that the inner bottom of the dish body 1 is provided with a slope of a middle bottom and a height around the bottom which diverges from the opening as the center.
[0037] The specific implementation process is as follows: after a molecular biology experiment is completed, the dish body 1 needs to be disinfected and sterilized. High-temperature sterilization will produce some water droplets accumulated at the bottom of the inner side of the dish body 1. After the transparent cover 3 is raised to a certain height using the same operation, the water droplets will naturally gather at the transparent cover 3 at the bottom of the slope due to the effect of the slope. Due to the presence of the injection and suction holes 4, the water droplets in the dish body 1 can be sucked by external suction. After the suction is completed, the transparent cover 3 is lowered using the same operation.
[0038] Example 3
[0039] The difference from the above embodiment is that a second rubber sleeve 16 is detachably provided on the top of the dish cover 15 , and the first rubber sleeve 2 is arranged at the bottom of the dish body 1 . Both the first rubber sleeve 2 and the second rubber sleeve 16 are made of elastic material.
[0040] The specific implementation process is as follows: after the disinfection of the dish body 1 is completed, the detachable first rubber sleeve 2 is put on the bottom of the dish body 1, and then the second rubber sleeve 16 is put on the top of the dish cover 15 to achieve the function of protecting the culture dish for storage.
[0041] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0042] The above is only an embodiment of the utility model. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the utility model before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for the technicians in this field, without departing from the structure of the utility model, several deformations and improvements can be made, which should also be regarded as the protection scope of the utility model, which will not affect the effect of the implementation of the utility model and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to explain the content of the claims.
Claims
1. A molecular biology culture dish, characterized in that: The invention comprises a culture dish, the bottom of which is provided with an opening, the culture dish is slidably connected with an injection and suction system through the opening, the injection and suction system comprises a transparent cover and an injection and suction tube, the transparent cover is fixedly connected with the injection and suction tube, the transparent cover is located at the bottom opening of the culture dish, the injection and suction tube is located at the lower part of the transparent cover, a plurality of injection and suction holes are opened on the side wall of the transparent cover, the injection and suction tube comprises a sealed sleeve and a cylinder body, the sealed sleeve is located at the upper part of the cylinder body and the lower part of the transparent cover, the sealed sleeve and the cylinder body are fixedly connected, an injection and suction tube is passed through the cylinder body, the injection and suction tube comprises a plurality of first injection and suction tubes and a second injection and suction tube, one end of the plurality of first injection and suction tubes respectively corresponds to the injection and suction holes, and the other end of the first injection and suction tubes is connected with the second injection and suction tubes.
2. The molecular biology culture dish according to claim 1, characterized in that: The culture dish comprises a dish body and a dish cover, and the dish body and the dish cover are threadedly connected.
3. The molecular biology culture dish according to claim 2, characterized in that: The bottom of the dish body is detachably connected with a first rubber sleeve, and the first rubber sleeve is provided with a through opening corresponding to the bottom of the dish body.
4. The molecular biology culture dish according to claim 3, characterized in that: The first rubber sleeve is provided with a plurality of support rods on four sides, and the support rods can be stored in the first rubber sleeve.
5. The molecular biology culture dish according to claim 4, characterized in that: A second rubber sleeve is arranged on the top of the dish cover, and both the first rubber sleeve and the second rubber sleeve are made of elastic material.
6. The molecular biology culture dish according to claim 5, characterized in that: The bottom of the inner side of the dish body is provided with a slope of a middle bottom and a height around the middle bottom which diverges to the surrounding areas with the opening as the center.
7. The molecular biology culture dish according to claim 6, characterized in that: The cylinder body comprises a first telescopic cylinder and a second telescopic cylinder. The first telescopic cylinder is slidably connected to the second telescopic cylinder inside, and a folding pull rod is fixedly connected to the bottom of the second telescopic cylinder.
8. The molecular biology culture dish according to claim 7, characterized in that: A spring clamping column is provided on the outer wall of the cylinder, and a clamping groove corresponding to the clamping head is provided on the inner wall of the bottom opening of the dish body opposite to the cylinder body. The spring clamping column includes a clamping head, a pressing head and a spring. The clamping head is located on the upper part of the spring clamping column. The clamping head and the pressing head are fixedly connected by a rod, and the spring is located at the bottom of the clamping head.