Special cassette for fluorescent staining of porous cell culture plate

Abstract

The utility model relates to a special dark box for fluorescent staining of a porous cell culture plate. The top cover is arranged at the top end of the light shielding box and comprises a pressing block and an arc-shaped convex surface, and the arc-shaped convex surface is arranged on the surface of one side of the pressing block; the placing frame is arranged in the light shielding box; the timer is arranged on the outer surface of the light shielding box through screws and comprises a timer body, a timing button and a zero clearing button, and the timing button and the zero clearing button are both arranged on the side, close to the arc-shaped protruding face, of the timer body. The timer, the top cover, the light shielding box and other components are arranged, the top cover is fixed to the top of the light shielding box through the hasp composed of the movable buckle and the fixed buckle, when the top cover is installed, the pressing block moves downwards in the direction of the vertical strip, and in the process, the reset button and the timing button are sequentially pressed by the arc-shaped protruding face; and the timer is reset first and then carries out timing.

Description

Technical Field

[0001] The utility model relates to the technical field of cell fluorescence staining equipment, in particular to a special dark box for fluorescence staining of a porous cell culture plate. Background Art

[0002] Cell fluorescence staining, also known as immunostaining, is a biotechnology for staining cells. Most of the staining operations require incubation in the dark, so they are usually performed in a dark room to avoid the influence of light on the staining process.

[0003] Since the setting of the darkroom is relatively demanding for the laboratory, and being in the darkroom with insufficient light for a long time will cause certain damage to the eyes of the experimenter, the Chinese patent CN220399071U discloses a cell culture plate dark box to solve the above problems. The patent sets a dark box so that the cell culture plate after the staining operation can be placed in a light-shielding box for incubation, so as to provide the light-proof environment required for cell staining;

[0004] However, the dark box in the above patent still has room for improvement. There are time requirements for staining cells in the culture plate. If the time is too short, it will lead to insufficient staining and fail to meet the actual use requirements. If the staining time is too long, problems such as uneven staining will occur. Therefore, when performing dark-proof cultivation, it is usually necessary to control the dark-proof time so that the dark-proof cultivation time is within the specified range, thereby ensuring that the staining effect is in the best state.

[0005] In order to ensure that the time of light-proof cultivation is appropriate, usually the experimenter will start timing when the cells are cultured in the light-proof plate. However, in actual situations, the experimenter may forget to count or the timing may be confused due to too many cells being cultured in the light-proof plate. Therefore, a special dark box for fluorescent staining of multi-well cell culture plates is proposed to solve the above problems. Utility Model Content

[0006] Based on the above description, the utility model provides a special dark box for fluorescent staining of multi-well cell culture plates to solve the problem that the existing dark boxes for cell culture plates do not have an automatic timing function.

[0007] The utility model provides a technical solution to the above-mentioned technical problem as follows: a dark box specially used for fluorescent staining of porous cell culture plates, comprising: a light-proof box; and a top cover, wherein the top cover is arranged at the top of the light-proof box, and comprises a pressing block and an arc-shaped raised surface, wherein the arc-shaped raised surface is arranged on the surface of one side of the pressing block, a placement rack is arranged inside the light-proof box, a timer is arranged on the outer surface of the light-proof box by screws, and comprises a timer body, a timing button and a reset button, wherein the timing button and the reset button are both arranged on one side of the timer body close to the arc-shaped raised surface.

[0008] On the basis of the above technical solution, the present invention can also be improved as follows.

[0009] Furthermore, the light-proof box comprises a main box body, the outer surface of the main box body is provided with vertical bars and threaded blind holes, and movable buckles are provided on both sides of the main box body.

[0010] Furthermore, the top cover includes a cover plate, and fixing buckles are arranged on both sides of the cover plate, and the fixing buckles and the movable buckles form a complete buckle.

[0011] Furthermore, a pressing block is provided on the outer surface of the cover plate, and an arc-shaped convex surface is provided on a side of the pressing block close to the timer, and the pressing block is arranged between the vertical bar and the timer.

[0012] Furthermore, a positioning protrusion is provided on the bottom wall of the cover plate, and the positioning protrusion extends to the inside of the main box body.

[0013] Furthermore, the timer includes a timer body, an outer surface of the timer body is provided with mounting ears, and the mounting ears are set on the outer surface of the main box body by screws.

[0014] Furthermore, a timing button and a reset button are provided on one side of the timer body close to the vertical bar, and the reset button is provided above the timing button.

[0015] Furthermore, the placement rack includes a placement plate, the upper surface of the placement plate is provided with a placement groove, and the lower surface of the placement plate is provided with support legs.

[0016] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0017] 1. The utility model is provided with components such as a timer, a top cover and a light-proof box, and the top cover is fixed on the top of the light-proof box through a buckle composed of a movable buckle and a fixed buckle. When the top cover is installed, the pressing block moves downward along the direction of the vertical bar. During this process, the arc-shaped convex surface presses the reset button and the timing button in turn, so that the timer is reset first and then the timing is performed;

[0018] 2. By setting the timer, the effect of timing is achieved by closing the dark box, so that the dark box can record the time of light-proof cultivation in time, avoiding the situation of forgetting to time and timing confusion, and by setting a placement rack, the dark box can place culture plates of different specifications in the dark. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the structure of a dark box dedicated to fluorescent staining of a multi-well cell culture plate provided in an embodiment of the utility model;

[0020] Figure 2 for Figure 1 A structural diagram from another perspective;

[0021] Figure 3 for Figure 1 Schematic diagram of the explosion structure;

[0022] Figure 4 This is a schematic diagram of the structure of the top cover in the embodiment of the utility model;

[0023] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0024] 1. Light-proof box; 11. Main box body; 12. Threaded blind hole; 13. Vertical bar; 14. Movable buckle; 2. Top cover; 21. Cover plate; 22. Fixing buckle; 23. Pressing block; 24. Arc-shaped raised surface; 25. Positioning protrusion; 3. Timer; 31. Timer body; 32. Mounting ear; 33. Timing button; 34. Reset button; 4. Placement rack; 41. Placement plate; 42. Placement slot; 43. Support leg. DETAILED DESCRIPTION

[0025] In order to facilitate understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the present application are provided in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0027] When used herein, the singular forms "a", "an", and "said / the" may also include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "include / comprise" or "have" etc. specify the presence of stated features, wholes, steps, operations, components, parts or combinations thereof, but do not exclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts or combinations thereof.

[0028] See also Figure 1 and Figure 2 , a dark box for fluorescent staining of a multi-well cell culture plate, comprising:

[0029] A light-proof box 1, comprising a main box body 11, the outer surface of which is provided with vertical bars 13 and threaded blind holes 12, and movable buckles 14 are provided on both sides of the main box body 11;

[0030] The top cover 2 is arranged at the top of the light-proof box 1 and comprises a pressing block 23 and an arc-shaped raised surface 24, wherein:

[0031] The top cover 2 includes a cover plate 21, and fixing buckles 22 are provided on both sides of the cover plate 21. The fixing buckles 22 and the movable buckle 14 form a complete buckle. An arc-shaped convex surface 24 is provided on the surface of one side of the pressing block 23. The outer surface of the cover plate 21 is provided with a pressing block 23, and the side of the pressing block 23 close to the timer 3 is provided with an arc-shaped convex surface 24. The pressing block 23 is arranged between the vertical bar 13 and the timer 3. The bottom wall of the cover plate 21 is provided with a positioning protrusion 25, and the positioning protrusion 25 extends to the inside of the main box body 11.

[0032] Based on the above, the light-proof box 1 and the top cover 2 are connected by a buckle composed of a fixed buckle 22 and a movable buckle 14. At this time, the light-proof box 1 and the top cover 2 form a complete box, providing a light-proof environment for the cell culture plate inside the light-proof box 1, and the vertical bar 13 plays a role in providing support for the pressing block 23 to prevent the pressing block 23 from being deformed when it contacts the timing button 33 and the reset button 34.

[0033] like Figure 2 As shown, the timer 3 is set on the outer surface of the light-proof box 1 by screws, and includes a timer body 31, a timing button 33 and a reset button 34, wherein:

[0034] The timer 3 includes a timer body 31, the outer surface of the timer body 31 is provided with a mounting ear 32, the mounting ear 32 is set on the outer surface of the main box body 11 by screws, a timing button 33 and a reset button 34 are both set on the side of the timer body 31 close to the arc-shaped convex surface 24, and the timer body 31 is provided with a timing button 33 and a reset button 34 on the side close to the vertical bar 13, and the reset button 34 is set above the timing button 33;

[0035] Based on the above, the timer 3 includes a timing module, a power supply, a display screen and other structures. Since the timer 3 is an existing mature technology, its specific working principle and structure are not described here. Only the button position is restricted so that the timer 3 can cooperate with the top cover 2, so that the top cover 2 and the light-proof box 1 are connected to each other and reset and timing actions are performed in sequence.

[0036] like Figure 2As shown, a placement rack 4 is arranged inside the light-proof box 1, and the placement rack 4 includes a placement plate 41, a placement groove 42 is arranged on the upper surface of the placement plate 41, and a support leg 43 is arranged on the lower surface of the placement plate 41;

[0037] Based on the above, the placement rack 4 is provided to support the cell culture plate to ensure that the cell culture plate is sufficiently stable when placed in the light-proof box 1, and by providing placement slots 42 of different specifications, the placement rack 4 can be suitable for culture plates of different sizes.

[0038] In actual use of this embodiment, the timer 3 is mounted on the surface of the main box body 11 by screws, and the placement rack 4 is placed inside the light-proof box 1, and the cell culture plate is placed inside the placement groove 42;

[0039] Then, by placing the top cover 2 on the top of the light-proof box 1, the pressing block 23 moves along the direction of the vertical bar 13. During the movement, the arc-shaped convex surface 24 presses the reset button 34 and the timing button 33 in sequence, so that the timer 3 is reset first, and then the timing is performed. At this time, the timing data is the specific time of the light-proof incubation. The specific time of the light-proof incubation can be known by reading the data on the display screen of the timer 3;

[0040] The dark box combines the reset and timing actions of the timer 3 with the installation action of the top cover 2, and starts timing when the top cover 2 is installed. While the timing is accurate, the test personnel will not forget to time or be confused in timing.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A dark box for fluorescent staining of multi-well cell culture plates, characterized in that: include: Light-proof box (1); as well as The top cover (2) is arranged at the top of the light-proof box (1) and comprises a pressing block (23) and an arc-shaped convex surface (24), wherein: An arc-shaped convex surface (24) is arranged on a surface of one side of the pressing block (23); A placement rack (4) arranged inside the light-proof box (1); The timer (3) is arranged on the outer surface of the light-proof box (1) by screws, and comprises a timer body (31), a timing button (33) and a reset button (34), wherein: The timing button (33) and the reset button (34) are both arranged on one side of the timer body (31) close to the arc-shaped convex surface (24).

2. The multi-well cell culture plate fluorescent staining special dark box according to claim 1, characterized in that: The light-proof box (1) comprises a main box body (11), the outer surface of the main box body (11) is provided with vertical bars (13) and threaded blind holes (12), and movable buckles (14) are provided on both sides of the main box body (11).

3. The dark box for fluorescent staining of multi-well cell culture plates according to claim 2, characterized in that: The top cover (2) comprises a cover plate (21), and fixing buckles (22) are arranged on both sides of the cover plate (21), and the fixing buckles (22) and the movable buckles (14) form a complete buckle.

4. The dark box for fluorescent staining of multi-well cell culture plates according to claim 3, characterized in that: A pressing block (23) is provided on the outer surface of the cover plate (21), and an arc-shaped convex surface (24) is provided on a side of the pressing block (23) close to the timer (3). The pressing block (23) is arranged between the vertical bar (13) and the timer (3).

5. The multi-well cell culture plate fluorescent staining special dark box according to claim 3, characterized in that: The bottom wall of the cover plate (21) is provided with a positioning protrusion (25), and the positioning protrusion (25) extends to the interior of the main box body (11).

6. The dark box for fluorescent staining of multi-well cell culture plates according to claim 2, characterized in that: The timer (3) comprises a timer body (31), the outer surface of the timer body (31) is provided with a mounting ear (32), and the mounting ear (32) is arranged on the outer surface of the main box body (11) by means of screws.

7. The dark box for fluorescent staining of multi-well cell culture plates according to claim 6, characterized in that: A timing button (33) and a reset button (34) are arranged on one side of the timer body (31) close to the vertical bar (13), and the reset button (34) is arranged above the timing button (33).

8. The multi-well cell culture plate fluorescent staining special dark box according to claim 1, characterized in that: The placement rack (4) comprises a placement plate (41), the upper surface of the placement plate (41) is provided with a placement groove (42), and the lower surface of the placement plate (41) is provided with a supporting leg (43).

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