Disinfection effect evaluation device of air disinfection device and aerosol cabinet thereof
By designing an air disinfection device disinfection effect evaluation device, and using simulated field test environment and natural environmental conditions to evaluate the disinfection effect of small one-time pass air disinfection devices, the problem of lack of scientific evaluation methods in the existing technology is solved, and the accurate evaluation and effectiveness of disinfection effect are achieved.
Patent Information
- Application Number
- CN202420490227.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-13
- Publication Date
- 2025-03-11
- Estimated Expiration
- 2034-03-13
AI Technical Summary
The existing technology lacks a scientific and comprehensive disinfection effect evaluation method for small single-use air disinfection devices, which leads to the inability to truly and objectively reflect its disinfection effect, increasing the potential risk of hospital infection.
An air disinfection device disinfection effect evaluation device is designed, including a simulated field test environmental group detection device and a natural environment group detection device. Through an aerosol cabinet and an air microbial sampling device, the disinfection effect of the air disinfection device to be evaluated under different environmental conditions is simulated.
The disinfection effect of small one-time pass air disinfection devices is accurately evaluated, which truly and objectively reflects its disinfection effect, fully guarantees the effectiveness of disinfection, and reduces the risk of hospital infection.
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Figure CN222593852U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of air disinfection effect evaluation, in particular to an air disinfection device disinfection effect evaluation device and an aerosol cabinet thereof. Background Art
[0002] In modern clinical medicine, how to reduce the safety risks caused by viruses contained in the exhaled gas from the exhaust end of equipment such as ventilators and prevent hospital infections is a major issue in the current medical field. As an effective means of artificially replacing the autonomous ventilation function, ventilators have been widely used in respiratory failure caused by various reasons, anesthesia respiratory management during major surgery, respiratory support therapy and emergency resuscitation. It is a vital medical device that can prevent and treat respiratory failure, reduce complications, and save and prolong patient lives. The gas discharged from the ventilator contains a large number of pathogenic bacteria and viruses. If it is directly discharged into the air without effective disinfection, it will cause the spread of pathogenic bacteria and viruses, which is easy to cause cross-infection in the hospital.
[0003] In order to cut off the contamination of indoor air by pathogenic bacteria and viruses in the patient's exhaled gas, the current conventional practice is to install a filtration system or purification system on the ventilator, or to install a disinfection unit based on short-wave ultraviolet rays, etc., to disinfect or purify the exhausted air, which has also been widely used in many medical institutions; however, up to now, there is no relevant authoritative method to scientifically and comprehensively evaluate the disinfection effect of these disinfection units. At present, the evaluation of the disinfection effect of these disinfection devices still uses the traditional evaluation method of large-volume air disinfection effect. This method is only applicable to the disinfection of circulating air in a closed space. There is no relevant standard method to scientifically and comprehensively evaluate the disinfection effect of this type of one-time disinfection unit. The existing evaluation method cannot truly and objectively reflect the actual disinfection scenario of these small disinfection devices, and cannot simulate the actual gas flow and flow rate of human breathing. In addition, the sample gas transported is cyclical, which makes the disinfection effect test results erroneous, bringing huge potential risks to the management of nosocomial infections in medical institutions. Therefore, it is urgent to establish a disinfection effect evaluation method suitable for the current small-scale disposable air disinfection device to truly and objectively reflect its disinfection effect, fully ensure the effectiveness of disinfection, and avoid the potential cross-infection risk caused by pathogenic bacteria and viruses in exhaled gas due to incomplete disinfection. Utility Model Content
[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide an air disinfection device disinfection effect evaluation device and an aerosol cabinet thereof, so as to solve the technical problem that there is no air disinfection effect detection method for a small disposable air disinfection device in the prior art.
[0005] In order to solve the above technical problems, a technical solution adopted by the utility model is: to provide an air disinfection device disinfection effect evaluation device, including: a simulated field test environment group detection device, a simulated field test environment control group detection device, a natural environment group detection device, a natural environment control group detection device, an aerosol bacteria contamination device, an air microorganism sampling device, a constant temperature incubator, and a computer system; the computer system establishes communication connections with the simulated field test environment group detection device, the simulated field test environment control group detection device, the natural environment group detection device, the natural environment control group detection device, the aerosol bacteria contamination device, and the air microorganism sampling device respectively; the simulated field test environment group The detection device comprises a first aerosol cabinet and a second aerosol cabinet; the first aerosol cabinet and the second aerosol cabinet are connected to the air disinfection device to be evaluated; the detection device of the control group of the simulated field test environment comprises a third aerosol cabinet and a fourth aerosol cabinet; the third aerosol cabinet and the fourth aerosol cabinet are connected to the control air disinfection device; wherein the control air disinfection device is an air disinfection device of the same model as the air disinfection device to be evaluated, with the disinfection factor removed or turned off; the detection device of the natural environment group comprises a fifth aerosol cabinet, which is connected to the air disinfection device to be evaluated; the detection device of the natural environment control group comprises a sixth aerosol cabinet, which is connected to the control air disinfection device.
[0006] Among them, each of the first aerosol cabinet, the second aerosol cabinet, the third aerosol cabinet, the fourth aerosol cabinet, the fifth aerosol cabinet and the sixth aerosol cabinet includes: a vent, a sampler connecting pipe, an ultraviolet disinfection lamp, a connecting pipe connection port, a cabinet door and a sleeve; the vent is arranged on the top surface of the aerosol cabinet and close to the edge of the top surface, wherein a sterile filter paper disc is installed; the sampler connecting pipe is arranged through the through hole arranged on the top surface of the aerosol cabinet and passes through the top surface, a part of the sampler connecting pipe is located in the external space of the aerosol cabinet to connect the air microorganism sampling device, and the other part is located in the internal space of the aerosol cabinet; the ultraviolet disinfection lamp is arranged in the internal space of the aerosol cabinet and close to the top surface of the aerosol cabinet; the connecting pipe connection port is arranged through the through hole arranged on the side of the aerosol cabinet and passes through the side, and the connecting pipe connection port is a hollow pipe inside, which is arranged in the external space of the side of the aerosol cabinet; the cabinet door is arranged on the side of the aerosol cabinet; the sleeve is arranged on the side of the aerosol cabinet.
[0007] Among them, the connecting pipe connection port provided by the first aerosol cabinet and the connecting pipe connection port provided by the second aerosol cabinet are connected to the air disinfection device to be evaluated; the connecting pipe connection port provided by the third aerosol cabinet and the connecting pipe connection port provided by the fourth aerosol cabinet are connected to the control air disinfection device; the connecting pipe connection port provided by the fifth aerosol cabinet is connected to the air disinfection device to be evaluated; the connecting pipe interface provided by the sixth aerosol cabinet is connected to the control air disinfection device.
[0008] Wherein, the aerosol cabinet further includes an agitator, which is arranged in the inner space of the aerosol cabinet and close to the bottom surface of the aerosol cabinet.
[0009] Among them, the first aerosol cabinet, the second aerosol cabinet, the third aerosol cabinet, the fourth aerosol cabinet, the fifth aerosol cabinet, and the sixth aerosol cabinet are all cabinets with a volume of 1 cubic meter, and the shape of the cabinet can be spherical, cubic, rectangular or cylindrical.
[0010] Wherein, the number of the vent hole is at least one, and is adjusted accordingly according to the flow rate of the air disinfection device to be evaluated.
[0011] Wherein, the sterile filter paper disc uses 0.45 μm or 0.22 μm water-soluble, sterile microporous filter paper (membrane).
[0012] Wherein, the cabinet door and the connecting pipe connection port are arranged on two different sides of the aerosol cabinet; the cabinet door and the sleeve are arranged on the same side of the aerosol cabinet.
[0013] Wherein, the air microorganism sampling device includes a six-stage impact sampler and a liquid impact sampler; the six-stage impact sampler and the liquid impact sampler are connected to the aerosol cabinet through the sampler connecting pipe.
[0014] In order to solve the above technical problems, another technical solution adopted by the utility model is: to provide an aerosol cabinet, including: a vent, a sampler connecting pipe, an ultraviolet disinfection lamp, a connecting pipe connection port, a cabinet door and a sleeve; the vent is arranged on the top surface of the aerosol cabinet and close to the edge of the top surface, wherein a sterile filter paper disc is installed; the sampler connecting pipe is arranged through the through hole arranged on the top surface of the aerosol cabinet, and a part of the sampler connecting pipe is located in the external space of the aerosol cabinet to connect the air microorganism sampling device, and the other part is located in the internal space of the aerosol cabinet; the ultraviolet disinfection lamp is arranged in the internal space of the aerosol cabinet and close to the top surface of the aerosol cabinet; the connecting pipe connection port is arranged through the through hole arranged on the side of the aerosol cabinet and passes through the side, and the connecting pipe connection port is a hollow pipe inside, which is arranged in the external space of the side of the aerosol cabinet; the cabinet door is arranged on the side of the aerosol cabinet; the sleeve is arranged on the side of the aerosol cabinet.
[0015] It can be seen from the above technical solutions that the implementation of the utility model has the following advantages:
[0016] A simulated field test environment group detection device and a simulated field test environment control group detection device consisting of two aerosol cabinets are pre-set, the air disinfection device to be evaluated is connected to the simulated field test environment group detection device, and the control air disinfection device is connected to the simulated field test environment control group detection device, and the aerosol cabinets of the two detection devices are sprayed with bacteria respectively under the conditions of the simulated field test environment, and the killing rates of the two detection devices are calculated respectively; at the same time, a natural environment group detection device and a natural environment control group detection device consisting of an aerosol cabinet are also pre-set, the air disinfection device to be evaluated is connected to the natural environment group detection device, and the control air disinfection device is connected to the natural environment control group detection device, and the killing rates of the two detection devices are calculated respectively under the natural environment; because the detection group and control group of the simulated field test environment and the detection group and control group of the natural environment are pre-set, the disinfection effect of the air disinfection device can be accurately evaluated based on the comprehensive test results, various influencing factors are fully considered, and the output of various influencing factor data is closely combined with the detection device to truly and objectively reflect the disinfection effect of the air disinfection device, thereby solving the technical problem that there is no air disinfection effect detection method for small disposable air disinfection devices in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.
[0018] Figure 1 It is a structural schematic diagram of a disinfection effect evaluation device for an air disinfection device in an embodiment of the utility model;
[0019] Figure 2 yes Figure 1 The structural schematic diagram of the detection device of the simulated field test environment group shown;
[0020] Figure 3 yes Figure 1 The structural schematic diagram of the natural environment group detection device is shown. DETAILED DESCRIPTION
[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0022] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back...), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.
[0023] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of the features. In addition, if "and / or" or "and / or" appears in the full text, its meaning includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or solutions that satisfy both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the utility model.
[0024] A disinfection effect evaluation device for an air disinfection device according to an embodiment of the utility model will be described in detail below in conjunction with the accompanying drawings.
[0025] See also Figure 1 , which is a schematic diagram of the structure of an air disinfection device disinfection effect evaluation device in the embodiment of the utility model. The evaluation device 10 includes: a simulated field test environment group detection device 11, a simulated field test environment control group detection device 12, a natural environment group detection device 13, a natural environment control group detection device 14, an aerosol contamination device 15, an air microorganism sampling device 16, a constant temperature incubator 17, and a computer system 18.
[0026] The environments of the simulated field test environment group detection device 11 and the simulated field test environment control group detection device 12 are consistent; wherein the environment includes temperature, humidity, light, airtightness and ventilation conditions, etc.
[0027] Specifically, the computer system 18 establishes communication connections with the simulated field test environment group detection device 11, the simulated field test environment control group detection device 12, the natural environment group detection device 13, the natural environment control group detection device 14, the aerosol contamination device 15, and the air microorganism sampling device 16 respectively.
[0028] Please also see Figure 2 Specifically, the simulated field test environment group detection device 11 includes: a first aerosol cabinet 110 and a second aerosol cabinet 111; the first aerosol cabinet 110 and the second aerosol cabinet 111 are connected to the air disinfection device 20 to be evaluated. In this embodiment, the first aerosol cabinet 110 and the second aerosol cabinet 111 are both cabinets with a volume of 1 cubic meter, and the cabinet shapes include but are not limited to spheres, cubes, cuboids and cylinders.
[0029] The first aerosol cabinet 110 includes: a first vent 1100 , a first sampler connecting pipe 1101 , an ultraviolet disinfection lamp 1102 , a first connecting pipe connection port 1103 , a first agitator 1104 , a first cabinet door 1105 and a first sleeve 1106 .
[0030] The first vent hole 1100 is provided on the top surface of the first aerosol cabinet 110 and near a vertex of the quadrilateral top surface, wherein a sterile filter paper disc is installed; the number of the first vent hole 1100 is at least one, which is adjusted and set according to the requirement of the flow rate of the air disinfection device 20 to be evaluated. In this embodiment, the sterile filter paper disc uses 0.45 μm or 0.22 μm sterile microporous filter paper (membrane), and generally uses water-soluble filter paper (membrane).
[0031] The first sampler connecting tube 1101 is arranged through a through hole opened on the top surface of the first aerosol cabinet 110 and passes through the top surface. A portion of the first sampler connecting tube 1101 is located in the external space of the first aerosol cabinet 110 to connect the sampler, and the other portion is located in the internal space of the first aerosol cabinet 110.
[0032] The ultraviolet disinfection lamp 1102 is arranged in the inner space of the first aerosol cabinet 110 and is arranged close to the top surface of the first aerosol cabinet 110; the ultraviolet disinfection lamp 1102 is used to be turned on after the whole test is completed, and irradiate and disinfect for 1 hour to finally disinfect the bacteria remaining on the surface of the first aerosol cabinet 110 and in the air.
[0033] The first communication pipe connection port 1103 is provided through a through hole provided on the side of the first aerosol cabinet 110 and penetrates the side. The first communication pipe connection port 1103 is a cylindrical, hollow pipe provided in the external space on the side of the first aerosol cabinet 110 .
[0034] The first agitator 1104 is disposed in the inner space of the first aerosol cabinet 110 and is disposed close to the bottom surface of the first aerosol cabinet 110 .
[0035] The first cabinet door 1105 is opened on the side of the first aerosol cabinet 110 and provides a window for sleeve operations such as spray infection and sampling. In this embodiment, the first cabinet door 1105 and the first connecting pipe connection port 1103 are arranged on different sides of the first aerosol cabinet 110.
[0036] The first sleeve 1106 is provided on a side of the first aerosol cabinet 110 , and is a window for sleeve operations such as sampling. In the present embodiment, the first cabinet door 1105 and the first sleeve 1106 are provided on the same side of the first aerosol cabinet 110 .
[0037] The second aerosol cabinet 111 includes: a second vent 1110 , a second sampler connecting pipe 1111 , an ultraviolet disinfection lamp 1112 , a second connecting pipe connection port 1113 , a second agitator 1114 , a second cabinet door 1115 and a second cuff 1116 .
[0038] The second vent hole 1110 is provided on the top surface of the second aerosol cabinet 111 and is close to a vertex corner of the quadrilateral top surface, wherein a sterile filter paper disc is installed; in the present embodiment, the sterile filter paper disc uses 0.45 μm or 0.22 μm sterile microporous filter paper (membrane), generally a water-soluble filter paper (membrane).
[0039] The second sampler connecting tube 1111 is arranged through a through hole on the top surface of the second aerosol cabinet 111 , a part of the second sampler connecting tube 1111 is located in the external space of the second aerosol cabinet 111 to connect the sampler, and the other part is located in the internal space of the second aerosol cabinet 111 .
[0040] The ultraviolet disinfection lamp 1112 is disposed in the inner space of the second aerosol cabinet 111 and is disposed close to the top surface of the second aerosol cabinet 111 .
[0041] The second communication pipe connection port 1113 is provided through a through hole provided on the side of the second aerosol cabinet 111 and penetrates the side. The second communication pipe connection port 1113 is a cylindrical, hollow pipe provided in the external space on the side of the second aerosol cabinet 111 .
[0042] The second agitator 1114 is disposed in the inner space of the second aerosol cabinet 111 and is disposed close to the bottom surface of the second aerosol cabinet 111 .
[0043] The second cabinet door 1115 is opened on the side of the second aerosol cabinet 111 and provides a window for sleeve operations such as spray infection and sampling. In this embodiment, the second cabinet door 1115 and the second connecting pipe connection port 1113 are arranged on two different sides of the second aerosol cabinet 111.
[0044] The second cuff 1116 is provided on a side of the second aerosol cabinet 111 and is a window for cuff operations such as sampling. In this embodiment, the second cabinet door 1115 and the second cuff 1116 are provided on the same side of the second aerosol cabinet 111 .
[0045] Furthermore, the air disinfection device 20 to be evaluated is connected between the first communicating pipe connection port 1103 and the second communicating pipe connection port 1113 .
[0046] Similarly, the simulated field test environment control group detection device 12 includes a third aerosol cabinet 120 and a fourth aerosol cabinet 121; the third aerosol cabinet 120 and the fourth aerosol cabinet 121 are connected to a control air disinfection device 20' (not shown). The control air disinfection device 20' and the air disinfection device 20 to be evaluated are air disinfection devices of the same model, with the disinfection factor removed or turned off. The structure and connection of the third aerosol cabinet 120 and the fourth aerosol cabinet 121 are Figure 2 The first aerosol cabinet 110 and the second aerosol cabinet 111 are the same and are not described in detail herein.
[0047] It should be noted that in the simulated field test environment control group detection device 12, the third connecting pipeline connection interface 1203 provided on the third aerosol cabinet 120 and the fourth connecting pipeline connection interface 1213 provided on the fourth aerosol cabinet 121 are connected to the control air disinfection device 20', and are not connected to the air disinfection device to be evaluated.
[0048] See also Figure 3 The natural environment group detection device 13 includes a fifth aerosol cabinet 130, which is connected to the air disinfection device to be evaluated 20. In this embodiment, the fifth aerosol cabinet 130 is a cabinet with a volume of 1 cubic meter, and the cabinet shape includes but is not limited to a sphere, a cube, a cuboid and a cylinder.
[0049] Similarly, the structure and connection of the fifth aerosol cabinet 130 are similar to Figure 2 The first aerosol cabinet 110 and the second aerosol cabinet 111 are the same and are not described in detail herein.
[0050] It should be noted that, in the natural environment group detection device 13 , the fifth connecting pipeline connection interface 1303 provided on the fifth aerosol cabinet 130 is connected to the air disinfection device 20 to be evaluated.
[0051] The natural environment control group detection device 14 includes a sixth aerosol cabinet 140. Similarly, the structure and connection of the sixth aerosol cabinet 140 are similar to those of the Figure 2 The first aerosol cabinet 110 and the second aerosol cabinet 111 are the same and are not described in detail herein.
[0052] It should be noted that, in the natural environment control group detection device 14, the sixth connecting pipeline connection interface 1403 provided on the sixth aerosol cabinet 140 is connected to the control air disinfection device 20'.
[0053] The aerosol bacteria contamination device 15 includes an air compressor, a pressure gauge, a gas flow meter and an aerosol bacteria contamination device, etc., and adopts the same structure as the prior art, which is not repeated here. More than 90% of the diameters of the bacterial aerosol particles are between 1 μm and 10 μm. The aerosol bacteria contamination device 15 is used to place the contamination device inside the aerosol cabinet through the aerosol cabinet door, and the aerosol output port should be placed in the center of the aerosol cabinet. The contamination device is started to inject sample bacteria.
[0054] The air microorganism sampling device 16 includes a six-stage impact sampler 161, a liquid impact sampler 162, and also includes an air extraction device, a gas flow meter, etc. (not shown in the figure), and adopts the same structure as the prior art, which is not repeated here. The air microorganism sampling device 16 is used to sample microorganisms in the air, and specifically includes but is not limited to a six-stage impact sampler 161, a first-level air sampler, an air extraction device, a gas flow meter, etc.
[0055] The constant temperature incubator 17 is used to culture culture dish samples containing culture medium collected from various air microorganism sampling devices 16 including liquid impact samplers 162, six-stage impact samplers 161, etc. The culture temperature is 36°C ± 1°C and the culture time is 24h to 48h.
[0056] The present utility model provides an air disinfection device disinfection effect evaluation device, and its specific working principle is as follows.
[0057] (I) Simulated field test environment detection
[0058] The air disinfection device 20 to be evaluated is installed on the connecting pipes of the first aerosol cabinet 110 and the second aerosol cabinet 111 of the simulated field test environment group detection device 11, and the power supply is connected and confirmed to be able to work normally. At the same time, a control air disinfection device 20' of the same model as the air disinfection device 20 to be evaluated and with the disinfection factor removed or turned off is installed on the connecting pipes of the third aerosol cabinet 120 and the fourth aerosol cabinet 121 of the simulated field test environment control group detection device 12. Then close the cabinet door of each aerosol cabinet so that the air disinfection device 20 to be evaluated forms a relatively independent closed passage with the first aerosol cabinet 110 and the second aerosol cabinet 111; similarly, the air disinfection device 20' forms a relatively independent closed passage with the third aerosol cabinet 120 and the fourth aerosol cabinet 121.
[0059] The computer system 18 is started to turn on the temperature and humidity adjustment device to ensure that the temperature and relative humidity inside the first aerosol cabinet 110, the second aerosol cabinet 111, the third aerosol cabinet 120 and the fourth aerosol cabinet 121 meet the temperature (20°C to 25°C) and relative humidity (50% to 70%) required by the test.
[0060] Sterile filter paper discs are installed on the ventilation holes of the first aerosol cabinet 110 , the second aerosol cabinet 111 , the third aerosol cabinet 120 and the fourth aerosol cabinet 121 in a sterile operation manner.
[0061] The computer system 18 controls the turning on of the ultraviolet disinfection lamps inside the first aerosol cabinet 110 , the second aerosol cabinet 111 , the third aerosol cabinet 120 , and the fourth aerosol cabinet 121 , and irradiates and disinfects for at least 30 minutes.
[0062] Take fresh slant cultures of the 3rd to 7th generations of Staphylococcus aureus that have been cultured at 36°C ± 1°C for 18h to 24h in advance, wash the bacterial lawn with TPS (tryptone saline solution), filter with sterile absorbent cotton, and dilute to the required concentration with nutrient broth medium before the experiment.
[0063] The computer system 18 sets the pressure, gas flow rate and spraying time of the aerosol bacteria contamination device 15 to control the aerosol bacteria contamination device 15 to spray and contaminate the microorganisms into the first aerosol cabinet 110 and the third aerosol cabinet 120 .
[0064] The computer system 18 also starts the first agitator 1104 in the first aerosol cabinet 110 to achieve spraying and stirring to accelerate the uniform diffusion of bacteria. After the spraying and infection is completed, the computer system 18 continues to control the first agitator 1104 to work for 5 minutes and then rest for 5 minutes.
[0065] The air microorganism sampling device 16 samples the environments in the first aerosol cabinet 110 and the third aerosol cabinet 120 respectively to obtain the total number of air colonies V01′ in the first aerosol cabinet 110 and the total number of air colonies V01 in the third aerosol cabinet 120 before disinfection.
[0066] The operator starts the air disinfection device 20 and disinfection device 20' to be evaluated and calculates the ventilation time. The total ventilation volume should be less than 0.5m 3 (1 / 2 volume of aerosol cabinet).
[0067] After the air disinfection device 20 and the disinfection device 20' to be evaluated have been working for a predetermined time, the operator shuts down the machine to stop working; the computer system 18 starts the air microorganism sampling device 16 (liquid impact sampler 162) to simultaneously sample the environments in the first aerosol cabinet 110, the second aerosol cabinet 111, the third aerosol cabinet 120 and the fourth aerosol cabinet 121 to obtain the total number of air colonies V'02 in the first aerosol cabinet 110 after disinfection, the total number of air colonies V02 in the third aerosol cabinet 120, the total number of air colonies Vt in the fourth aerosol cabinet 121, the total number of colonies Vs on the filter paper (membrane) in the fourth aerosol cabinet 121, the total number of air colonies V't in the second aerosol cabinet 111, and the total number of colonies V's on the filter paper (membrane) in the second aerosol cabinet 111.
[0068] It should be noted that sampling the sterile filter paper disc of the fourth aerosol cabinet 121 is used to collect bacteria that have settled or escaped outside the system. Specifically, the sterile filter paper disc of the fourth aerosol cabinet 121 is removed by aseptic operation, placed in a sterile triangular flask with glass beads containing 50 ml of phosphate buffer, eluted, and sampled by the air microorganism sampling device 16 (liquid impact sampler 162) to obtain the total colony count Vs on the filter paper (membrane) in the fourth aerosol cabinet 121 after disinfection and the total colony count V's on the filter paper (membrane) in the second aerosol cabinet 111. The computer system 18 calculates the killing rate of bacteria by the air disinfection device based on the total number of air colonies in each aerosol cabinet as described above and the pre-set calculation method.
[0069] After sampling, the operator takes out the culture dish containing the culture medium after sampling, places it in an incubator at 36°C±1°C for 48 hours, and then counts the viable bacteria. If the air disinfection device 20 to be evaluated uses chemical disinfection factors, a neutralizer that has been verified to be qualified in the early stage must be added to the culture medium.
[0070] After completing the sampling of the simulated field test environment group detection device 11 and the simulated field test environment control group detection device 12, the unused culture medium of the same batch is cultured together with the above two groups of samples as a negative control. If bacteria grow in the negative control group, it means that the culture medium used is contaminated, the test is invalid, and the sterile equipment is replaced and repeated.
[0071] Repeat the above-mentioned operation to obtain three groups of test data, and when the killing rates calculated three times are not less than 99.9%, it can be determined that the air disinfection device 20 to be evaluated is qualified for disinfection.
[0072] (II) Natural environment test and detection
[0073] The air disinfection device 20 to be evaluated is installed on the connecting pipe of the fifth aerosol cabinet 130 of the natural environment group detection device 13, and the power supply is connected and confirmed to be able to work normally. At the same time, a control air disinfection device 20' of the same model as the air disinfection device 20 to be evaluated and with the disinfection factor removed or turned off is installed on the connecting pipe of the sixth aerosol cabinet 140 of the natural environment control group detection device 14.
[0074] Sterile filter paper discs are installed on the vents of the fifth aerosol cabinet 130 and the sixth aerosol cabinet 140 in a sterile manner.
[0075] The computer system 18 controls the turning on of the ultraviolet disinfection lamps inside the fifth aerosol cabinet 130 and the sixth aerosol cabinet 140 for irradiation and disinfection for 60 minutes.
[0076] The sampling port of the air microorganism sampling device 16 is placed at a horizontal position within 50 cm of the air inlet (wind) port of the air disinfection device 20 to be evaluated for sampling. The sampling time is preferably 5 minutes. After bacterial culture, live bacteria count and calculation, the bacterial content A of the external environment air before disinfection can be obtained.
[0077] The air microorganism sampling device 16 is connected to the fifth aerosol cabinet 130 and the sixth aerosol cabinet 140 for sampling, and the sampling time is preferably 5 minutes. After bacterial culture, live bacteria counting and calculation, the bacterial content B of the ambient air in the cabinet before disinfection can be obtained.
[0078] The operator starts the air disinfection device 20 and the disinfection device 20' to be evaluated, calculates the working time T, and obtains the air flow rate L of the device to be tested.
[0079] After the air disinfection device 20 and the disinfection device 20' to be evaluated have been working for a predetermined time, the operator shuts down the machine to stop working; the computer system 18 starts the air microorganism sampling device 16 (six-stage impact sampler 161) to sample the bacteria in the air in the fifth aerosol cabinet 130 and the sixth aerosol cabinet 140, and the sampling time is preferably less than 15 minutes. Among them, the parameters of the samples collected by the six-stage impact sampler 161 are set according to the sampler instruction manual, and the operation is performed outdoors through a window with a sealed sleeve. After bacterial culture, live bacteria counting and calculation, the bacterial content C of the ambient air in the cabinet after disinfection can be obtained.
[0080] Remove the sterile filter paper discs from the inner layer of the fifth aerosol cabinet 130 and the sixth aerosol cabinet 140 by aseptic operation, put them into a sterile conical flask with glass beads containing 50 ml of sodium phosphate buffer, wash them, and culture them with bacteria. After counting and calculating the live bacteria, the total colony count D on the inner filter membrane of the aerosol cabinet can be obtained.
[0081] After sampling, the operator places the culture dish in a 36℃±1℃ incubator for 48 hours to count the live bacteria. After completing the sampling of the natural environment group detection device 13 and the natural environment control group detection device 14, the unused culture medium of the same batch is cultured at the same time as the above two groups of samples as a negative control. If bacteria grow in the negative control group, it means that the culture medium used is contaminated, the test is invalid, and the sterile equipment is replaced and repeated.
[0082] The computer system 18 calculates the killing rate of bacteria by the air disinfection device according to the total number of air colonies in each aerosol cabinet as described above and a preset calculation method.
[0083] Repeat the above-mentioned operation to obtain three groups of test data, and when the killing rates calculated three times are not less than 90%, it can be determined that the air disinfection device 20 to be evaluated is qualified for disinfection.
[0084] Finally, based on the test results of the simulated on-site test environment test and the natural environment test test, it is determined that the air disinfection device 20 to be evaluated is qualified for disinfection.
[0085] The advantages of the utility model are as follows:
[0086] A simulated field test environment group detection device and a simulated field test environment control group detection device consisting of two aerosol cabinets are pre-set, the air disinfection device to be evaluated is connected to the simulated field test environment group detection device, and the control air disinfection device is connected to the simulated field test environment control group detection device, and the aerosol cabinets of the two detection devices are sprayed with bacteria respectively under the conditions of the simulated field test environment, and the killing rates of the two detection devices are calculated respectively; at the same time, a natural environment group detection device and a natural environment control group detection device consisting of an aerosol cabinet are also pre-set, the air disinfection device to be evaluated is connected to the natural environment group detection device, and the control air disinfection device is connected to the natural environment control group detection device, and the killing rates of the two detection devices are calculated respectively under the natural environment; because the detection group and control group of the simulated field test environment and the detection group and control group of the natural environment are pre-set, the disinfection effect of the air disinfection device can be accurately evaluated based on the comprehensive detection results, various influencing factors are fully considered, and the output of various influencing factor data is closely combined with the detection device to truly and objectively reflect the disinfection effect of the air disinfection device, thereby solving the technical problem that there is no air disinfection effect detection method for small disposable air disinfection devices in the prior art.
[0087] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.
Claims
1. A device for evaluating the disinfection effect of an air disinfection device, characterized in that: include: A detection device for a simulated field test environment group, a detection device for a simulated field test environment control group, a detection device for a natural environment group, a detection device for a natural environment control group, an aerosol bacteria contamination device, an air microorganism sampling device, a constant temperature incubator, and a computer system; the computer system establishes communication connections with the detection device for the simulated field test environment group, the detection device for the simulated field test environment control group, the detection device for the natural environment group, the detection device for the natural environment control group, the aerosol bacteria contamination device, and the air microorganism sampling device, respectively; The simulated field test environment group detection device comprises a first aerosol cabinet and a second aerosol cabinet; the first aerosol cabinet and the second aerosol cabinet are connected to the air disinfection device to be evaluated; The simulated field test environment control group detection device comprises a third aerosol cabinet and a fourth aerosol cabinet; the third aerosol cabinet and the fourth aerosol cabinet are connected to a control air disinfection device; wherein the control air disinfection device is an air disinfection device of the same model as the air disinfection device to be evaluated, with the disinfection factor removed or turned off; The natural environment group detection device includes a fifth aerosol cabinet connected to the air disinfection device to be evaluated; The natural environment control group detection device includes a sixth aerosol cabinet, which is connected to the control air disinfection device.
2. The air disinfection device disinfection effect evaluation device according to claim 1, characterized in that: Each of the first aerosol cabinet, the second aerosol cabinet, the third aerosol cabinet, the fourth aerosol cabinet, the fifth aerosol cabinet and the sixth aerosol cabinet comprises: a vent, a sampler connecting pipe, an ultraviolet disinfection lamp, a connecting pipe connection port, a cabinet door and a sleeve; The vent hole is arranged on the top surface of the aerosol cabinet and close to the edge of the top surface, and a sterile filter paper disc is installed therein; The sampler connecting pipe is arranged through a through hole formed on the top surface of the aerosol cabinet and passes through the top surface. A part of the sampler connecting pipe is located in the external space of the aerosol cabinet to connect with the air microorganism sampling device, and another part is located in the internal space of the aerosol cabinet. The ultraviolet disinfection lamp is arranged in the inner space of the aerosol cabinet and is arranged close to the top surface of the aerosol cabinet; The connecting pipe connection port is provided by a through hole provided on the side of the aerosol cabinet and penetrates the side. The connecting pipe connection port is a hollow pipe provided in the outer space of the side of the aerosol cabinet. The cabinet door is opened on the side of the aerosol cabinet; The sleeve is arranged on the side of the aerosol cabinet.
3. The air disinfection device disinfection effect evaluation device according to claim 2, characterized in that: The connecting pipe connection port provided in the first aerosol cabinet and the connecting pipe connection port provided in the second aerosol cabinet are connected to the air disinfection device to be evaluated; The control air disinfection device is connected between the connecting pipe connection port provided in the third aerosol cabinet and the connecting pipe connection port provided in the fourth aerosol cabinet; The connecting pipe connection port provided in the fifth aerosol cabinet is connected to the air disinfection device to be evaluated; The connection pipeline interface provided on the sixth aerosol cabinet is connected to the control air disinfection device.
4. The air disinfection device disinfection effect evaluation device according to claim 3, characterized in that: The aerosol cabinet further comprises a stirrer, which is arranged in the inner space of the aerosol cabinet and close to the bottom surface of the aerosol cabinet.
5. The air disinfection device disinfection effect evaluation device according to claim 3, characterized in that: The first aerosol cabinet, the second aerosol cabinet, the third aerosol cabinet, the fourth aerosol cabinet, the fifth aerosol cabinet and the sixth aerosol cabinet are all cabinets with a volume of 1 cubic meter, and the shape of the cabinet can be spherical, cubic, rectangular or cylindrical.
6. The air disinfection device disinfection effect evaluation device according to claim 5, characterized in that: The number of the vent hole is at least one, and is adjusted accordingly according to the flow rate of the air disinfection device to be evaluated.
7. The air disinfection device disinfection effect evaluation device according to claim 3, characterized in that: The sterile filter paper disc uses 0.45 μm or 0.22 μm water-soluble, sterile microporous filter paper.
8. The air disinfection device disinfection effect evaluation device according to claim 5, characterized in that: The cabinet door and the connecting pipe connection port are arranged on two different sides of the aerosol cabinet; The cabinet door and the sleeve are arranged on the same side of the aerosol cabinet.
9. The air disinfection device disinfection effect evaluation device according to claim 3, characterized in that: The air microorganism sampling device comprises a six-stage impact sampler and a liquid impact sampler; the six-stage impact sampler and the liquid impact sampler are connected to the aerosol cabinet via the sampler connecting pipe.
10. An aerosol cabinet, characterized in that: include: Ventilation holes, sampler connection tubes, ultraviolet disinfection lamps, connecting pipe connections, cabinet doors, and sleeves; The vent hole is arranged on the top surface of the aerosol cabinet and close to the edge of the top surface, and a sterile filter paper disc is installed therein; The sampler connecting pipe is arranged through a through hole formed on the top surface of the aerosol cabinet and passes through the top surface. A part of the sampler connecting pipe is located in the external space of the aerosol cabinet to connect with the air microorganism sampling device, and the other part is located in the internal space of the aerosol cabinet. The ultraviolet disinfection lamp is arranged in the inner space of the aerosol cabinet and is arranged close to the top surface of the aerosol cabinet; The connecting pipe connection port is provided by a through hole provided on the side of the aerosol cabinet and penetrates the side. The connecting pipe connection port is a hollow pipe provided in the outer space of the side of the aerosol cabinet. The cabinet door is opened on the side of the aerosol cabinet; The sleeve is arranged on the side of the aerosol cabinet.