Cell incubator

By using the culture medium to move the structure and the culture medium to hold the structure in the cell incubator, the problems of temperature unevenness and the entry of external bacteria are solved, the temperature uniformity and environmental isolation of cell culture are achieved, and the quality and consistency of cell culture are improved.

CN222631456UActive Publication Date: 2025-03-18ORVIS (FUJIAN) CELL BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420978390.9
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-08
Publication Date
2025-03-18
Estimated Expiration
2034-05-08

AI Technical Summary

Technical Problem

Due to the uneven temperature in the box and the problem of external bacteria entering, the growth rate of cells in the culture medium at different locations is different, affecting cell culture.

Method used

A cell incubator was designed, using two rows of medium moving structures and medium holding structures to realize the movement and isolation of the medium through a transmission belt and a push device, reducing temperature differences and preventing the entry of external bacteria.

Benefits of technology

The structure is moved by the culture medium, the medium temperature is evenly controlled, and the difference in cell growth rate is reduced; the structure is placed through the culture medium, the external environment is isolated, bacteria are prevented from entering, and the quality and consistency of cell culture are improved.

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Abstract

The utility model discloses a cell culture box which comprises a culture box main body, a glass door arranged at the front end of the culture box main body, a box door positioned at the front end of the glass door and a controller positioned at the front end of the top of the culture box main body, the culture medium moving structure comprises a plurality of transmission belts, each transmission belt is composed of a support, a driving roller, a plurality of driven rollers and a belt body, the support on one transmission belt close to one side of the inner wall of the incubator main body is fixedly connected with a driving motor, and the driving rollers on the plurality of transmission belts are fixedly connected through a connecting shaft; and the connecting shaft is fixedly connected with a rotating shaft of the driving motor. Through the arrangement of the culture medium moving structure, the difference of the growth speed of cells in the culture medium at different positions can be reduced; by arranging a culture medium taking and placing structure, external bacteria can be prevented from entering the culture box to influence cell growth.
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Description

Technical Field

[0001] The utility model relates to a cell culture device, in particular to a cell culture box. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] At present, the cell culture incubator used for cell culture has a certain error in the temperature of the culture medium at different positions due to the uneven temperature inside the incubator, resulting in different cell growth rates in the culture medium at different positions. Secondly, when the incubator is opened to take out or put in the culture medium, the outside air enters the device, and the bacteria in the outside air will enter the incubator, affecting the cell culture. Therefore, we have made improvements to this and proposed a cell culture incubator. Utility Model Content

[0004] In order to solve the defects in the prior art, the utility model provides a cell culture box.

[0005] In order to solve the above technical problems, the utility model provides the following technical solutions:

[0006] The utility model discloses a cell culture box, comprising a culture box body, a glass door arranged at the front end of the culture box body, a box door located at the front end of the glass door, and a controller located at the front end of the top of the culture box body. Two rows of culture medium moving structures are arranged inside the culture box body, and culture medium picking and placing structures are arranged on the tops of the two rows of culture medium moving structures. The culture medium picking and placing structures are composed of a moving device and a pushing device located at the bottom of the moving device. An ultraviolet lamp located at the top of a support plate is fixedly arranged on the inner wall of the isolation cover, and a taking window arranged opposite to the pushing device is arranged at the front end of the glass door through a hinge movement.

[0007] As a preferred technical solution of the utility model, the culture medium moving structure includes several transmission belts, which are composed of a bracket, an active roller, several driven rollers and a belt body. The bracket on one of the transmission belts close to the inner wall of the incubator body is fixedly connected to the drive motor, and the active rollers on the several transmission belts are fixedly connected through a connecting shaft, and the connecting shaft is fixedly connected to the rotating shaft of the drive motor. Two oppositely arranged L-shaped rods are fixedly provided at the top of the transmission belt, and one end of the two L-shaped rods is provided with a ring, and a culture medium tray is provided between the rings, and sliding grooves are opened on both sides of the culture medium tray, and the rings are located in the sliding grooves.

[0008] As a preferred technical solution of the utility model, the pushing and conveying device includes an isolation cover, a screw is provided inside the isolation cover, and the screw is composed of a screw, a sliding block sleeved on the screw, and a stabilizing rod passing through the sliding block. A pushing motor is fixedly provided at the rear end of the isolation cover, and the rotating shaft of the pushing motor passes through the isolation cover and is fixedly connected to the screw, the stabilizing rod is fixedly connected to the isolation cover, and a support plate is fixedly provided at the front end of the sliding block.

[0009] As a preferred technical solution of the utility model, the moving device includes an X-axis electric slide, a Y-axis electric slide and a Z-axis electric telescopic rod. The Y-axis electric slide is fixedly connected to the bottom end of the slider on the X-axis electric slide, the Z-axis electric telescopic rod is fixedly connected to one side of the slider on the Y-axis electric slide, and the upper push rod of the Z-axis electric telescopic rod is fixedly connected to the top of the isolation cover.

[0010] As a preferred technical solution of the utility model, a magnet sheet is fixedly provided on the top of the support plate, and an iron sheet is provided in the middle of the bottom end of the culture medium tray.

[0011] As a preferred technical solution of the utility model, a rubber ring is adhered to the rear end of the box door.

[0012] As a preferred technical solution of the utility model, two groups of cold light lamps are provided at the top of the interior of the incubator body.

[0013] As a preferred technical solution of the utility model, a universal wheel is embedded at the bottom end of the incubator body, and a spring is sleeved on the support rod of the universal wheel.

[0014] The beneficial effects of the utility model are as follows: the cell culture box:

[0015] 1. The culture medium moving structure can drive the culture medium to move back and forth inside the incubator during the cell culture process, and also reduce the contact area between the culture medium and the device, so as to reduce the temperature difference of each culture medium, thereby reducing the difference in cell growth rate in the culture medium at different positions;

[0016] 2. The culture medium taking and placing structure can separate the interior of the incubator from the external environment when placing and taking out the culture medium, thus preventing external bacteria from entering the incubator and affecting cell growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0018] Figure 1 This is a structural schematic diagram of a cell culture box of the utility model;

[0019] Figure 2 It is a structural schematic diagram of a transmission belt of a cell culture box of the utility model;

[0020] Figure 3 It is a structural schematic diagram of a front view cross section of a culture box body of a cell culture box of the utility model;

[0021] Figure 4 This is a structural schematic diagram of a glass door of a cell culture box of the utility model;

[0022] Figure 5 The utility model is a structural schematic diagram of a pushing device of a cell culture box.

[0023] In the figure: 1. incubator body; 2. door; 3. glass door; 31. picking window; 4. controller; 5. culture medium moving structure; 51. transmission belt; 52. drive motor; 53. L-shaped rod; 54. ring; 6. culture medium tray; 7. culture medium picking and placing structure; 71. X-axis electric slide; 72. Y-axis electric slide; 73. Z-axis electric telescopic rod; 74. isolation cover; 75. push motor; 76. screw; 77. stabilizer rod; 78. sliding block; 79. support plate; 710. ultraviolet lamp. DETAILED DESCRIPTION

[0024] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0025] Example: Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5As shown, the utility model is a cell culture box, comprising a culture box body 1, a glass door 3 arranged at the front end of the culture box body 1, a box door 2 located at the front end of the glass door 3, and a controller 4 located at the top front end of the culture box body 1. Two rows of culture medium moving structures 5 are arranged inside the culture box body 1. The culture medium moving structure 5 includes a plurality of transmission belts 51, and the transmission belts 51 are composed of a bracket, an active roller, a plurality of driven rollers and a belt body. A bracket on one side of the transmission belt 51 close to the inner wall of the culture box body 1 is fixedly connected to a driving motor 52, and the active rollers on the plurality of transmission belts 51 are fixedly connected through a connecting shaft, and the connecting shaft is fixedly connected to the rotating shaft of the driving motor 52. Two L-shaped rods 53 arranged opposite to each other are fixedly arranged at the top of the transmission belt 51, and a ring 54 is sleeved on one end of the two L-shaped rods 53, and a culture medium tray 6 is arranged between the rings 54. Slide grooves are provided on both sides of the culture medium tray 6, and the rings 54 are located in the slide grooves. A culture medium picking and placing structure 7 is provided on the top of the two rows of culture medium moving structures 5. The culture medium picking and placing structure 7 consists of a moving device and a pushing device located at the bottom of the moving device. The pushing and sending device includes an isolation cover 74, and a screw rod is provided inside the isolation cover 74. The screw rod consists of a screw rod 76, a sliding block 78 sleeved on the screw rod 76, and a stabilizing rod 77 passing through the sliding block 78. A pushing motor 75 is fixedly provided at the rear end of the isolation cover 74, and the rotating shaft of the pushing motor 75 passes through the isolation cover 74 and is fixedly connected to the screw rod 76. The stabilizing rod 77 is fixedly connected to the isolation cover 74. A support plate 79 is fixedly provided at the front end of the sliding block 78. An ultraviolet lamp 710 located on the top of the support plate 79 is fixedly provided on the inner wall of the isolation cover 74. The front end of the glass door 3 is provided with a taking window 31 arranged opposite to the pushing device through a hinge.

[0026] Among them, the moving device includes an X-axis electric slide 71, a Y-axis electric slide 72 and a Z-axis electric telescopic rod 73. The Y-axis electric slide 72 is fixedly connected to the bottom end of the slider on the X-axis electric slide 71, the Z-axis electric telescopic rod 73 is fixedly connected to one side of the slider on the Y-axis electric slide 72, and the push rod on the Z-axis electric telescopic rod 73 is fixedly connected to the top of the isolation cover 74 to realize the control of the isolation cover 74.

[0027] Among them, a magnet sheet is fixedly provided on the top of the support plate 79, and an iron sheet is provided in the middle of the bottom end of the culture medium tray 6 to prevent the culture medium from falling when taking out the culture medium.

[0028] Among them, a rubber ring is adhered to the rear end of the box door 2 to improve the sealing performance of the device.

[0029] Among them, two groups of cold light lamps are arranged at the top of the interior of the incubator body 1 to illuminate the interior of the device.

[0030] A universal wheel is embedded at the bottom of the incubator body 1, and a spring is sleeved on the support rod of the universal wheel to reduce shock during movement.

[0031] During operation, first sterilize and disinfect the inside of the incubator body 1, then set the temperature and humidity through the controller 4, then place the corresponding ring 54 of the slide groove of the culture medium on the culture medium moving structure 5, and then close the glass door 3, drive the motor 52 to drive the belt body on the transmission belt 51 to move, thereby driving the culture medium tray 6 to move through the L-shaped rod 53, and the center of gravity of the culture medium tray 6 is at the bottom, ensuring that the culture medium tray 6 is always open upward, ensuring that the temperature of each culture medium is not much different, and reducing the difference in cell growth rate in the culture medium at different positions. When taking out the culture medium, use the moving device to drive the isolation cover 74 to move, so that the isolation cover 74 is located behind the culture medium to be taken out, and the push motor 75 uses the screw rod to push the support plate 79 forward to the culture medium. The base, then the moving device drives the isolation cover 74 to move to the corresponding picking window 31. During the movement, the pushing motor 75 is reversed to move the support plate 79 backward, so that the culture medium is located inside the isolation cover 74. The isolation cover 74 covers the picking window 31, controls the support plate 79 to push forward, pushes open the picking window 31 and extends out through the glass door 3, then removes the culture medium, and the support plate 79 returns to the inside of the isolation cover 74. The picking window 31 is closed, and the ultraviolet lamp 710 is turned on to sterilize the inside of the isolation cover 74. The isolation cover 74 returns to its original position. When putting in the culture medium, the culture medium is placed on the support plate 79 after the support plate 79 passes through the glass door 3, and then the isolation cover 74 is driven by the moving device to place the culture medium on the culture medium moving structure 5, and the placement position is the same as at the beginning.

[0032] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A cell culture box, comprising a box body (1), a glass door (3) arranged at the front end of the box body (1), a box door (2) located at the front end of the glass door (3), and a controller (4) located at the top front end of the box body (1), characterized in that: Two rows of culture medium moving structures (5) are provided inside the incubator body (1), and culture medium holding and placing structures (7) are provided on the tops of the two rows of culture medium moving structures (5), wherein the culture medium holding and placing structures (7) are composed of a moving device and a pushing device located at the bottom of the moving device; The pushing device comprises an isolation cover (74), a screw rod is arranged inside the isolation cover (74), and the screw rod is composed of a screw rod (76), a sliding block (78) sleeved on the screw rod (76), and a stabilizing rod (77) penetrating the sliding block (78); a pushing motor (75) is fixedly arranged at the rear end of the isolation cover (74), a rotating shaft of the pushing motor (75) passes through the isolation cover (74) and is fixedly connected to the screw rod (76), the stabilizing rod (77) is fixedly connected to the isolation cover (74), and a supporting plate (79) is fixedly arranged at the front end of the sliding block (78); An ultraviolet lamp (710) located on the top of the support plate (79) is fixedly provided on the inner wall of the isolation cover (74), and a taking window (31) arranged opposite to the pushing device is provided at the front end of the glass door (3) through a hinge.

2. A cell culture incubator according to claim 1, characterized in that: The culture medium moving structure (5) comprises a plurality of transmission belts (51), wherein the transmission belts (51) are composed of a bracket, a driving roller, a plurality of driven rollers and a belt body, wherein a bracket on one of the transmission belts (51) close to the inner wall of the culture box body (1) is fixedly connected to a driving motor (52), and the driving rollers on the plurality of transmission belts (51) are fixedly connected via a connecting shaft, and the connecting shaft is fixedly connected to the rotating shaft of the driving motor (52), and two oppositely arranged L-shaped rods (53) are fixedly provided at the top end of the transmission belt (51), and one end of the two L-shaped rods (53) is sleeved with a ring (54), and a culture medium tray (6) is provided between the rings (54), and slide grooves are provided on both sides of the culture medium tray (6), and the rings (54) are located in the slide grooves.

3. A cell culture incubator according to claim 2, characterized in that: The moving device comprises an X-axis electric slide (71), a Y-axis electric slide (72) and a Z-axis electric telescopic rod (73); the Y-axis electric slide (72) is fixedly connected to the bottom end of a slider on the X-axis electric slide (71); the Z-axis electric telescopic rod (73) is fixedly connected to one side of the slider on the Y-axis electric slide (72); and the push rod on the Z-axis electric telescopic rod (73) is fixedly connected to the top end of the isolation cover (74).

4. A cell culture incubator according to claim 3, characterized in that: A magnet sheet is fixedly provided at the top end of the support plate (79), and an iron sheet is provided at the middle of the bottom end of the culture medium tray (6).

5. A cell culture incubator according to claim 1, characterized in that: A rubber ring is adhered to the rear end of the box door (2).

6. A cell culture incubator according to claim 1, characterized in that: Two groups of cold light lamps are provided at the top of the interior of the incubator body (1).

7. A cell culture incubator according to claim 1, characterized in that: A universal wheel is embedded at the bottom end of the incubator body (1), and a spring is sleeved on a support rod of the universal wheel.