Device capable of quantitatively and simultaneously supplementing liquid for multi-batch cell culture

Abstract

The utility model discloses a device capable of quantitatively supplementing liquid for multi-batch cell culture simultaneously, which comprises a base, one side of the upper surface of the base is movably connected with a tank body, the top of the outer surface of the tank body is in threaded connection with a top cover, the inside of the top cover is movably connected with a liquid extraction pipe, and one end of the liquid extraction pipe is fixedly connected with a filter head. According to the device capable of quantitatively supplementing liquid for multi-batch cell culture at the same time, through the arrangement of a liquid pumping pipe, a peristaltic pump, a liquid discharging pipe, a flow dividing box and a quantitative ring, the peristaltic pump pumps a culture solution in the tank body through the liquid pumping pipe, the culture solution is conveyed into the flow dividing box through the liquid discharging pipe for flow dividing, and finally the culture solution is conveyed into a culture bag through a fixing pipe; the quantitative loop is used for controlling the flow of the culture solution, so that each culture bag can obtain the culture solution with the same volume, quantitative liquid supplementing can be continuously carried out, the liquid supplementing efficiency is improved, meanwhile, the culture solution is prevented from being exposed in the air, and the infection condition during cell culture is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a device capable of quantitatively replenishing multiple batches of cells at the same time. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell growth requires a nutritional environment. The nutrient matrix used to maintain cell growth is called a culture medium, which is divided into culture fluid and culture solid. In the process of culturing cells with culture fluid, cells will consume the culture fluid, so it is necessary to add culture fluid to the cell culture bag.

[0003] After searching, Chinese patent publication (announcement) number CN210438747U discloses a fluid replenishment device for cell culture, including a culture bottle, a liquid adding cylinder detachably fixedly connected to the culture bottle, the liquid adding cylinder and the culture bottle are in a connected state; the liquid adding cylinder is provided with an internal thread, and a piston threadedly connected to the internal thread is installed in the liquid adding cylinder. By adding culture fluid to the culture bottle through the liquid adding cylinder, the culture fluid can be added without opening the culture bottle, which effectively reduces the probability of cell contamination.

[0004] Although the above patent adds culture fluid to the culture bottle through a liquid adding cylinder, when culturing cells, new nutrients need to be added to the cell bottle manually at any time. The culture fluid cannot be added automatically, and the amount of culture fluid added cannot be quantified, which is inconvenient to use. Therefore, it is necessary to design a device that can quantitatively replenish fluid for multiple batches of cell culture at the same time to solve the above problems. Utility Model Content

[0005] The main purpose of the utility model is to provide a device for quantitatively replenishing multiple batches of cell cultures at the same time, which can effectively solve the problems in the background technology.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A device capable of quantitatively replenishing multiple batches of cells at the same time comprises a base, one side of the upper surface of the base is movably connected to a tank body, the top of the outer surface of the tank body is threadedly connected to a top cover, the inside of the top cover is movably connected to a liquid extraction tube, one end of the liquid extraction tube is fixedly connected to a filter head; the other end of the liquid extraction tube is fixedly connected to a peristaltic pump, one end of the peristaltic pump is fixedly connected to a liquid discharge tube, one end of the liquid discharge tube is movably connected to a diversion box, the lower surface of the diversion box is fixedly connected to a fixed tube, the outer surface of the fixed tube is fixedly connected to a quantitative ring, and the upper surface of the base close to the fixed tube is movably connected to a culture bag.

[0008] In order to facilitate the fixation of the peristaltic pump, the utility model is a device for quantitatively replenishing multiple batches of cell cultures at the same time. A fixing plate is fixedly connected to the edge of the upper surface of the base, and a bracket is fixedly connected to the front side of the fixing plate close to the peristaltic pump.

[0009] In order to facilitate checking of the liquid level changes, as a device for quantitatively replenishing multiple batches of cell cultures at the same time, a liquid level window is fixedly connected to the middle of the front side of the tank body.

[0010] In order to facilitate the adjustment of the position of the splint, the utility model is a device for quantitatively replenishing multiple batches of cell cultures at the same time. The outer surface of the base is fixedly connected to a motor, the output end of the motor is fixedly connected to a bidirectional screw, and both sides of the outer surface of the bidirectional screw are threadedly connected to sliders.

[0011] In order to facilitate the fixing of the tank, as a device for quantitatively replenishing multiple batches of cell cultures at the same time, the upper surface of the slider is fixedly connected with a clamping plate, and the inner side of the clamping plate is provided with an anti-slip groove.

[0012] In order to facilitate the support of the culture bag, as a device for quantitatively replenishing multiple batches of cells at the same time, a partition is fixedly connected to one side of the upper surface of the base close to the culture bag.

[0013] In order to achieve the effect of facilitating the penetration of leaked culture fluid, the utility model provides a device for quantitatively replenishing multiple batches of cell cultures at the same time. A groove is provided on the upper surface of the base close to the culture bag, and a through hole is provided on the inner bottom wall of the groove.

[0014] In order to facilitate the collection of waste liquid, as a device for quantitatively replenishing multiple batches of cell cultures at the same time, the base is slidably connected to a collection box inside, and a handle is fixedly connected to the outer surface of the collection box.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] 1. In the utility model, through the arrangement of the liquid extraction tube, the peristaltic pump, the liquid discharge tube, the diversion box and the quantitative ring, the peristaltic pump extracts the culture fluid in the tank body through the liquid extraction tube, transports the culture fluid to the inside of the diversion box through the liquid discharge tube for diversion, and finally transports it to the inside of the culture bag through the fixed tube. The quantitative ring controls the flow rate of the culture fluid to ensure that each culture bag can obtain an equal volume of culture fluid, can quickly replenish the fluid, and can continuously and quantitatively replenish the fluid, thereby improving the efficiency of replenishment and avoiding the culture fluid from being exposed to the air, thereby reducing the infection during cell culture and improving the accuracy of the cell culture process.

[0017] 2. In the utility model, through the arrangement of partitions, grooves, through holes and collecting boxes, the partitions support and fix the diversion box, and multiple culture bags are placed in a classified manner. At the same time, the culture bags are supported to avoid tipping over during use, thereby ensuring stability in use. The grooves prevent the culture solution from being randomly spilled during fluid replenishment, and the spilled culture solution is discharged to the collecting box through multiple through holes, thereby reducing pollution to the experimental environment and further improving the use effect of the device. The waste liquid in the collecting box is collected for centralized treatment, thereby increasing convenience in use. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the main structure of an embodiment of the utility model;

[0019] Figure 2 It is a schematic diagram of the cross-sectional plan structure of an embodiment of the utility model;

[0020] Figure 3 This is a schematic diagram of the internal structure of the tank and the diverter box of an embodiment of the utility model;

[0021] Figure 4 This is a schematic diagram of the base structure of an embodiment of the utility model;

[0022] Figure 5 This is a schematic diagram of the internal structure of the base according to an embodiment of the utility model.

[0023] In the figure: 1. base; 2. tank body; 3. top cover; 4. suction tube; 5. filter head; 6. peristaltic pump; 7. discharge tube; 8. diversion box; 9. fixed tube; 10. quantitative ring; 11. culture bag; 12. fixed plate; 13. bracket; 14. liquid level window; 15. motor; 16. bidirectional screw; 17. slider; 18. splint; 19. anti-slip groove; 20. partition; 21. groove; 22. through hole; 23. collection box; 24. handle. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Example

[0026] like Figure 1-5As shown, a device for quantitatively replenishing multiple batches of cells at the same time comprises a base 1, one side of the upper surface of the base 1 is movably connected to a tank body 2, the top of the outer surface of the tank body 2 is threadedly connected to a top cover 3, the inside of the top cover 3 is movably connected to a liquid extraction tube 4, one end of the liquid extraction tube 4 is fixedly connected to a filter head 5; the other end of the liquid extraction tube 4 is fixedly connected to a peristaltic pump 6, one end of the peristaltic pump 6 is fixedly connected to a liquid discharge tube 7, one end of the liquid discharge tube 7 is movably connected to a diversion box 8, the lower surface of the diversion box 8 is fixedly connected to a fixed tube 9, the outer surface of the fixed tube 9 is fixedly connected to a quantitative ring 10, and the upper surface of the base 1 is movably connected to a culture bag 11 near the fixed tube 9.

[0027] When in use, the culture fluid is stored in the tank body 2 through the arrangement of the liquid extraction tube 4, the peristaltic pump 6, the discharge tube 7, the diversion box 8 and the quantitative ring 10. The top cover 3 is threadedly connected to the outer surface of the tank body 2, which is convenient for installation and disassembly, and is convenient for cleaning the tank body 2 after use. The cleaning is more convenient to avoid errors in the experiment caused by mixing different culture fluids. The bag mouth of the culture bag 11 is sleeved on the outer surface of the fixed tube 9. The peristaltic pump 6 extracts the culture fluid inside the tank body 2 through the liquid extraction tube 4, and the filter head 5 eliminates impurities such as bubbles in the culture fluid. The culture fluid is filtered to ensure the rigor of the experiment, and then the culture fluid is transported to the inside of the diversion box 8 through the discharge pipe 7 for diversion, and finally transported to the inside of the culture bag 11 through the fixed pipe 9. The quantitative ring 10 controls the flow of the culture fluid to ensure that each culture bag 11 can obtain an equal volume of culture fluid, can quickly replenish fluid, and can continuously perform quantitative replenishment, thereby improving the efficiency of replenishment and avoiding the culture fluid from being exposed to the air, thereby reducing the infection during cell culture, improving the accuracy of the cell culture process, and improving the effect of cell culture.

[0028] In this embodiment, a fixing plate 12 is fixedly connected to the edge of the upper surface of the base 1 , and a bracket 13 is fixedly connected to the front side of the fixing plate 12 close to the peristaltic pump 6 .

[0029] During specific use, the fixing plate 12 is provided to support and fix the diversion box 8 , and the bracket 13 plays a role in supporting and fixing the peristaltic pump 6 .

[0030] In this embodiment, a liquid level window 14 is fixedly connected to the middle of the front side of the tank body 2 .

[0031] During specific use, by providing the liquid level window 14 , it is convenient for the staff to check the change of the culture liquid inside the tank body 2 from the outside through the liquid level window 14 , so as to make timely adjustments.

[0032] In this embodiment, a motor 15 is fixedly connected to the outer surface of the base 1 , a bidirectional screw 16 is fixedly connected to the output end of the motor 15 , and sliders 17 are threadedly connected to both sides of the outer surface of the bidirectional screw 16 .

[0033] During specific use, through the setting of the bidirectional screw 16, the motor 15 drives the bidirectional screw 16 to rotate, so that the two clamping plates 18 on the outer surface are moved closer to or separated from each other, so as to facilitate the taking and placing of the can body 2.

[0034] In this embodiment, a clamping plate 18 is fixedly connected to the upper surface of the sliding block 17 , and an anti-slip groove 19 is formed on the inner side surface of the clamping plate 18 .

[0035] During specific use, the two clamping plates 18 are provided to clamp and fix the tank body 2 to ensure the stability of the device during operation, and the anti-slip groove 19 further increases the clamping effect.

[0036] In this embodiment, a partition plate 20 is fixedly connected to one side of the upper surface of the base 1 close to the culture bag 11 .

[0037] During specific use, the partition 20 is provided to support and fix the diversion box 8, and multiple culture bags 11 are placed in a classified manner. At the same time, the culture bags 11 are supported to avoid tipping during use, thereby ensuring stability in use.

[0038] In this embodiment, a groove 21 is formed on a side of the upper surface of the base 1 close to the culture bag 11 , and a through hole 22 is formed on the inner bottom wall of the groove 21 .

[0039] During specific use, the groove 21 is arranged to prevent the culture solution from being randomly spilled during liquid replenishment. The spilled culture solution is discharged to the collection box 23 through multiple through holes 22, thereby reducing pollution to the experimental environment and further improving the use effect of the device.

[0040] In this embodiment, a collection box 23 is slidably connected to the interior of the base 1 , and a handle 24 is fixedly connected to the outer surface of the collection box 23 .

[0041] During specific use, by setting the collecting box 23, the collecting box 23 is slidably connected to the inside of the base 1, and the waste liquid left through the through hole 22 can be collected for centralized treatment. The handle 24 is used to facilitate pulling out the collecting box 23, thereby increasing the convenience of use.

[0042] Working principle: During use, the culture fluid is transported to the inside of the tank body 2, and the top cover 3 is threadedly connected to the outer surface of the tank body 2, which is convenient for cleaning the tank body 2 after use to avoid mixing of different culture fluids and causing errors in the experiment. The motor 15 drives the bidirectional screw 16 to rotate, so that the two clamps 18 clamp and fix the tank body 2, and the bag mouth of the culture bag 11 is set on the outer surface of the fixed tube 9. The peristaltic pump 6 extracts the culture fluid inside the tank body 2 through the suction tube 4, and the filter head 5 eliminates and filters the bubbles and other impurities in the culture fluid, and transports the culture fluid to the inside of the diversion box 8 through the drainage tube 7 for diversion, and finally transports it to the inside of the culture bag 11 through the fixed tube 9. The quantitative ring 10 The flow rate of the liquid is controlled to ensure that each culture bag 11 can obtain an equal volume of culture liquid, can quickly replenish the liquid, and can continuously replenish the liquid quantitatively, while avoiding the culture liquid from being exposed to the air, thereby reducing the infection during cell culture. Through the liquid level window 14, it is convenient for the staff to check the change of the culture liquid inside the tank body 2 from the outside. The partition 20 classifies and places more than one culture bag 11, and at the same time supports the culture bag 11 to avoid tipping during use. The groove 21 prevents the culture liquid from being randomly spilled during replenishment. The spilled culture liquid is discharged to the collection box 23 through multiple through holes 22, reducing the pollution to the experimental environment and further improving the use effect of the device.

[0043] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A device capable of quantitatively replenishing multiple batches of cell cultures simultaneously, comprising a base (1), characterized in that: A tank body (2) is movably connected to one side of the upper surface of the base (1); a top cover (3) is threadedly connected to the top of the outer surface of the tank body (2); a liquid extraction tube (4) is movably connected inside the top cover (3); and a filter head (5) is fixedly connected to one end of the liquid extraction tube (4); The other end of the liquid extraction tube (4) is fixedly connected to a peristaltic pump (6), one end of the peristaltic pump (6) is fixedly connected to a liquid discharge tube (7), one end of the liquid discharge tube (7) is movably connected to a diversion box (8), the lower surface of the diversion box (8) is fixedly connected to a fixed tube (9), the outer surface of the fixed tube (9) is fixedly connected to a quantitative ring (10), and the upper surface of the base (1) is movably connected to a culture bag (11) on a side close to the fixed tube (9).

2. The device for quantitatively replenishing multiple batches of cell cultures simultaneously according to claim 1, characterized in that: A fixing plate (12) is fixedly connected to the edge of the upper surface of the base (1), and a bracket (13) is fixedly connected to the front side of the fixing plate (12) close to the peristaltic pump (6).

3. The device for quantitatively replenishing multiple batches of cells at the same time according to claim 1, characterized in that: A liquid level window (14) is fixedly connected to the middle of the front side of the tank body (2).

4. The device for quantitatively replenishing multiple batches of cells at the same time according to claim 1, characterized in that: The outer surface of the base (1) is fixedly connected to a motor (15), the output end of the motor (15) is fixedly connected to a bidirectional screw (16), and both sides of the outer surface of the bidirectional screw (16) are threadedly connected to sliders (17).

5. The device for quantitatively replenishing multiple batches of cell cultures simultaneously according to claim 4, characterized in that: A clamping plate (18) is fixedly connected to the upper surface of the sliding block (17), and an anti-slip groove (19) is provided on the inner side surface of the clamping plate (18).

6. The device for quantitatively replenishing multiple batches of cells at the same time according to claim 1, characterized in that: A partition plate (20) is fixedly connected to one side of the upper surface of the base (1) close to the culture bag (11).

7. The device for quantitatively replenishing multiple batches of cells at the same time according to claim 1, characterized in that: A groove (21) is provided on a side of the upper surface of the base (1) close to the culture bag (11), and a through hole (22) is provided on the inner bottom wall of the groove (21).

8. The device for quantitatively replenishing multiple batches of cells at the same time according to claim 1, characterized in that: The interior of the base (1) is slidably connected to a collection box (23), and the outer surface of the collection box (23) is fixedly connected to a handle (24).

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