Stem cell incubator suitable for automatic cell culture

Abstract

The embodiment of the utility model discloses a stem cell culture box suitable for automatic cell culture, which comprises a culture box body and a cell factory carrier, the culture body comprises a box body, a culture box door and a door opening and closing mechanism, one side of the box body is provided with an opening, and the other side of the box body is provided with an opening. The incubator door is connected to an opening of the incubator body through the door opening and closing mechanism, a carbon dioxide control assembly and an in-place sensor are installed in the incubator body, a ventilation connector is installed on the carbon dioxide control assembly, a guide rail is installed in the incubator body, and the carbon dioxide control assembly is connected with the in-place sensor. And the cell factory carrier is respectively connected with the guide rail and the ventilation interface. By implementing the stem cell culture box disclosed by the embodiment of the utility model, the problems of slow carbon dioxide concentration adjustment, dependence on manual operation and the like in the traditional cell culture box can be solved, and the cell culture efficiency and quality are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture boxes, in particular to a stem cell culture box suitable for automated cell culture. Background Art

[0002] Traditional cell culture incubators use ventilation systems to control the gas composition inside the incubator, usually by injecting pure carbon dioxide gas into the interior and mixing it with the internal air to achieve the desired carbon dioxide concentration. This process requires the use of computing devices such as chips to ensure the accuracy of the gas composition.

[0003] However, in traditional incubators, gas mixing takes time, usually 8 hours or even longer for the carbon dioxide concentration in each layer inside the entire incubator to reach the desired level. Such a delay may affect the growth rate and quality of cells, thereby reducing production efficiency and product quality. In traditional cell culture incubators, manual intervention is essential. The cell factory needs to be manually placed in the incubator and taken out when needed. This manual-dependent operation may not only affect the stability of the culture environment, but is also not suitable for large-scale production automation needs.

[0004] Therefore, it is necessary to design a new incubator to solve the problems of slow carbon dioxide concentration regulation and dependence on manual operation in traditional cell culture incubators, and to improve the efficiency and quality of cell culture. Utility Model Content

[0005] The purpose of the utility model is to overcome the defects of the prior art and provide a stem cell culture box suitable for automated cell culture.

[0006] In order to solve the above technical problems, the purpose of the utility model is achieved through the following technical solutions: providing a stem cell culture box suitable for automated cell culture, including: a culture box body and a cell factory carrier, the culture body including a box body, a culture box door and a door opening and closing mechanism, one side of the box body is provided with an opening, the culture box door is connected to the opening of the box body through the door opening and closing mechanism, a carbon dioxide control component and an in-situ sensor are installed in the box body, a ventilation interface is installed on the carbon dioxide control component, a guide rail is installed in the box body, and the cell factory carrier is respectively connected to the guide rail and the ventilation interface.

[0007] A further technical solution is as follows: the box body and the door opening and closing mechanism are combined to form a cavity, a guide rail mounting plate is installed at the bottom of the cavity, and the guide rail is connected to the guide rail mounting plate.

[0008] A further technical solution is as follows: the lower end of the box is connected with an adjustable supporting foot and a positioning pin.

[0009] Its further technical solution is: the door opening and closing mechanism includes a door shaft seat, a door shaft, a door opening motor assembly, a door opening sensor plate and a door opening angle sensor, the door shaft seat is installed on the box body, the door shaft is installed on the door shaft seat, the incubator door is connected to the door opening motor assembly, the door opening sensor plate is connected to the incubator door, and the door opening angle sensor is installed on the door shaft.

[0010] A further technical solution is as follows: a heat insulation strip is installed on one side of the incubator door close to the box body.

[0011] A further technical solution is that a sealing strip is installed at the opening of the box.

[0012] A further technical solution is as follows: an air duct and a fan assembly are installed on the upper end surface of the cavity, and the fan assembly is connected to the air duct.

[0013] A further technical solution is as follows: the box is provided with an interface, a touch screen and an indication alarm light.

[0014] Its further technical solution is: the cell factory carrier includes: a carrier body, a cell factory fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body, the clamping structure is connected to the manipulator, and the gas-sealed docking structure is connected to the ventilation interface; a guide roller is installed under the carrier body, and the guide roller is in rolling contact with the guide rail.

[0015] A further technical solution is as follows: the cell factory fixing structure comprises a limit mounting plate, a knob spring pin, and a locking knob; the knob spring pin and the locking knob are respectively connected to the limit mounting plate.

[0016] Compared with the prior art, the utility model has the following beneficial effects: the utility model provides an incubator body and a cell factory carrier, wherein the incubator body includes an incubator body, an incubator door and a door opening and closing mechanism, wherein the door opening and closing mechanism can automatically open or close the incubator door, and an in-situ sensor can accurately control the ventilation and concentration of carbon dioxide by a carbon dioxide control component after detecting that the cell factory carrier is in place, and a guide rail is provided to facilitate the movement of the cell factory carrier into or out of the box, thereby solving the problems of slow carbon dioxide concentration adjustment and dependence on manual operation in traditional cell incubators, and improving the efficiency and quality of cell culture.

[0017] The utility model is further described below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 1 ;

[0020] Figure 2 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 2 ;

[0021] Figure 3 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 3 ;

[0022] Figure 4 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 4 ;

[0023] Figure 5 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 5 ;

[0024] Figure 6 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 6 ;

[0025] Figure 7 Schematic diagram of the three-dimensional structure of the cell factory carrier provided in the embodiment of the utility model Figure 1 ;

[0026] Figure 8 Schematic diagram of the three-dimensional structure of the cell factory carrier provided in the embodiment of the utility model Figure 2 ;

[0027] Fig. 9 A schematic diagram of a three-dimensional structure of a cell factory carrier and a cell factory connected to each other provided by an embodiment of the utility model;

[0028] Description of the symbols in the figure:

[0029] 1. Carrier body; 2. Locking knob; 3. Locking position adjustment plate; 4. Knob spring pin; 5. Roller; 6. Pagoda air pipe joint; 7. Air pipe limit seat; 8. Connecting pipe; 9. Air guide plate; 10. Limit mounting plate; 11. Clamping plate; 12. Cell factory; 13. Hose; 14. Sterile filter; 15. Box; 16. Sealing strip; 17. Door shaft cover; 18. Box door; 19. Insulation strip; 2 0. Cell factory carrier; 21. Ventilation interface; 22. Guide rail; 23. Guide rail mounting plate; 24. Adjustable support foot; 25. Positioning pin; 26. Air duct; 27. Carbon dioxide control component; 28. In-position sensor; 29. ​​Interface; 30. Touch screen; 31. Indicator alarm light; 32. Door shaft seat; 33. Door shaft; 34. Door opening motor assembly; 35. Door opening sensor plate; 36. Door opening angle sensor. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0031] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0032] It should also be understood that the terms used in this utility model specification are only for the purpose of describing specific embodiments and are not intended to limit the utility model. As used in this utility model specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0033] It should be further understood that the term “and / or” used in the present specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0034] See also Figure 1 , Figure 1 A schematic diagram of a three-dimensional structure of a stem cell culture box suitable for automated cell culture provided in an embodiment of the utility model Figure 1It can be used in scenarios of automatic cell culture to solve problems such as slow carbon dioxide concentration adjustment and reliance on manual operation in traditional cell culture incubators, thereby improving the efficiency and quality of cell culture.

[0035] See also Figure 1 The above-mentioned stem cell culture box suitable for automated cell culture includes: a culture box body and a cell factory carrier 20, the culture box body includes a box body 15, a culture box door 18 and a door opening and closing mechanism, one side of the box body 15 is provided with an opening, the culture box door 18 is connected to the opening of the box body 15 through the door opening and closing mechanism, a carbon dioxide control component 27 and an in-situ sensor are installed in the box body 15, a ventilation interface 21 is installed on the carbon dioxide control component 27, a guide rail 22 is installed in the box body 15, and the cell factory carrier 20 is respectively connected to the guide rail 22 and the ventilation interface 21.

[0036] The incubator is equipped with a carbon dioxide control component 27 and an in-situ sensor, and carbon dioxide is directly introduced into the cell factory through the ventilation interface 21. The incubator has a circulation pipeline such as an air duct 26 and a fan component, equipped with a carbon dioxide concentration sensor and an air pressure sensor, which can control parameters such as ventilation volume, cycle time, and number of cycles through a program, and quickly adjust the carbon dioxide concentration of each layer inside the cell factory; the incubator has facilities such as a positioning plate, a positioning pin 25, and a door opening and closing mechanism, which can achieve precise positioning, automatic door opening and closing, and assist the manipulator in accurately guiding the placement of the cell factory carrier 20.

[0037] The automated operation of the stem cell culture incubator of this embodiment reduces manual intervention, making the cell culture process more stable and efficient, and improving production efficiency; by precisely controlling the carbon dioxide concentration, the carbon dioxide concentration of each layer inside the cell factory can quickly reach the required value, which is beneficial to ensuring the quality and rate of cell growth; the design of the automated cell culture incubator meets the needs of large-scale production and can meet the requirements of automated operation and efficient production; the functional design has been verified through actual operation testing and has high reliability, ensuring the stability and reliability of the cell culture process.

[0038] Overall, this Root Cell Culture Incubator aims to solve the problems of slow gas mixing and reliance on manual operation in traditional cell culture processes by providing precise CO2 control, automated operation, and efficient production functions, thereby improving production efficiency and product quality.

[0039] In one embodiment, see Figures 1 to 3 The box 15 and the door opening and closing mechanism are combined to form a cavity, and a guide rail mounting plate 23 is installed at the bottom of the cavity, and the guide rail 22 is connected to the guide rail mounting plate 23. The setting of the guide rail 22 can make it easier for the cell factory carrier 20 to move into the cavity and out of the cavity.

[0040] In one embodiment, see Figures 1 to 5 The lower end of the box body 15 is connected with an adjustable support foot 24 and a positioning pin 25.

[0041] In this embodiment, the adjustable support feet 24 are installed below the box body 15 of the incubator body, and the horizontality of the incubator can be adjusted. The positioning pins 25 are connected to the mounting plate and installed on the box body 15, and the installation position of the box body 15 can be accurately positioned, so that the entire box body 15 is suitable for automated operation.

[0042] By adjusting the height of the support feet, the incubator can be kept level on different surfaces, helping to ensure the stability and accuracy of the cell culture process; by inserting the positioning pins 25 into the corresponding holes in the mounting plate, the box body 15 can be accurately positioned during installation, making the entire box body 15 suitable for automated operation.

[0043] The addition of these design details makes the incubator more stable and reliable in automated operation. The adjustable support feet 24 ensure the stability of the box 15 on different platforms, while the positioning pins 25 ensure the accurate positioning of the box 15 during installation, so that automated operation can be performed more accurately and efficiently.

[0044] In one embodiment, the door opening and closing mechanism includes a door shaft seat 32, a door shaft 33, a door opening motor assembly 34, a door opening sensor plate 35 and a door opening angle sensor 36. The door shaft seat 32 is installed on the box body 15, the door shaft 33 is installed on the door shaft seat 32, the incubator door 18 is connected to the door opening motor assembly 34, the door opening sensor plate 35 is connected to the incubator door 18, and the door opening angle sensor 36 is installed on the door shaft 33.

[0045] In this embodiment, a door shaft cover 17 is installed outside the door shaft seat 32. The door shaft 33 is a hollow door shaft 33; the cable in the incubator door 18 can enter the box body 15 through the hollow door shaft 33.

[0046] The door shaft seat 32 is mounted on the box body 15, and the door shaft 33 is mounted on the door shaft seat 32. These two components together constitute the switch bearing structure of the door of the box body 15; the door opening motor assembly 34 is a motor assembly used to control the opening and closing of the door, and is connected to the incubator door 18; the door opening sensor plate 35 is connected to the incubator door 18, and is used to detect the state of the door or as a sensor to trigger the door opening and closing action; the door opening angle sensor 36 is mounted on the box body 15, and is used to detect the opening angle of the door or to control the door opening and closing action; the door shaft cover 17 is mounted on the outside of the door shaft seat 32, and is used to protect the door shaft 33 and related components. The door shaft 33 is hollow in design, so that the cable in the incubator door 18 can enter the box body 15 through the hollow door shaft 33.

[0047] The door opening motor assembly 34 and related sensors can be used to automatically open and close the incubator door 18, which facilitates operation and maintenance. The design of the hollow door shaft 33 allows the cables in the incubator door 18 to pass through it and enter the box body 15, which facilitates equipment connection and maintenance. The structural design of the door shaft seat 32, the door shaft 33, etc. makes the door opening and closing action more stable and reliable.

[0048] The addition of these design details enables the incubator door to be operated automatically, improving operational convenience and system stability. It also takes into account the convenience of equipment connection and maintenance, providing users with a better user experience.

[0049] In one embodiment, see Figures 1 to 4 A heat insulation strip 19 is installed on one side of the incubator door 18 close to the box body 15 .

[0050] In this embodiment, the insulation strip 19 is used to isolate the external ambient temperature and ensure the stability of the internal temperature of the incubator, which is beneficial for biological culture or other applications requiring a constant temperature environment; this helps save energy, avoid heat loss, and provide more stable biological culture conditions.

[0051] In one embodiment, see Figures 1 to 4 A sealing strip 16 is installed at the opening of the box body 15.

[0052] In this embodiment, the sealing strip 16 is used to ensure the sealing of the box 15 to prevent air or moisture from leaking out, and also to prevent microorganisms or foreign matter from entering the box 15. This helps to maintain a clean and stable environment inside the incubator.

[0053] In one embodiment, see Figure 6 The upper end surface of the above-mentioned cavity is installed with an air duct 26 and a fan assembly, and the fan assembly is connected to the air duct 26.

[0054] In this embodiment, the air duct 26 and the fan assembly are used to circulate air, adjust temperature or provide ventilation function; the fan assembly is connected to the air duct 26 to realize air flow, which is conducive to maintaining uniform temperature and humidity inside the incubator.

[0055] In one embodiment, see Figure 5 The box 15 is provided with an interface 29 , a touch screen 30 and an indication alarm light 31 .

[0056] In this embodiment, the interface 29, the touch screen 30 and the indicator alarm light 31 provide a user operation interface and status display, so that the user can conveniently monitor and control the working status of the incubator; the indicator alarm light 31 can intuitively display the operating status of the incubator and remind the user of relevant information. The interface 29 refers to the gas, electricity and network interface 29.

[0057] In this embodiment, the thermal insulation strip 19 and the sealing strip 16 help to ensure the stability of the temperature inside the incubator and provide good biological culture conditions; the design of the thermal insulation strip 19 and the sealing strip 16 can reduce energy waste, improve energy efficiency, and reduce operating costs; the air duct 26 and the fan assembly provide air flow and temperature and humidity adjustment functions, which are conducive to maintaining the uniformity and stability of the environment inside the incubator; the interface 29, the touch screen 30 and the indicator alarm light 31 provide an intuitive operation interface and status display, which improves the user's operation convenience and safety.

[0058] In one embodiment, the cell factory carrier 20 includes: a carrier body 1, a cell factory 12 fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory 12 fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body 1, and the clamping structure is connected to the robot.

[0059] In this embodiment, the carrier body 1 is a bracket with four hollow sides and a supporting surface on the bottom. The cell factory 12 is placed from the top of the carrier body 1, which is conducive to sufficient heat exchange of the cell factory 12 in the box 15 to ensure that the temperature of each layer of cells remains consistent.

[0060] In one embodiment, see Figure 7 A guide roller 5 is installed below the above-mentioned carrier, and the guide roller 5 is in rolling contact with the guide rail 22 in the box body 15.

[0061] In this embodiment, there are four guide rollers 5. Of course, the number of guide rollers 5 can be set according to actual conditions. The setting of the guide rollers can cooperate with the guide rails 22 in the box body 15 to easily move the carrier into the incubator and accurately connect the gas sealing docking structure with the gas pipe joint in the incubator.

[0062] In one embodiment, see Figure 7 and Fig. 9 The above-mentioned cell factory 12 fixing structure includes a limit mounting plate 10, a knob spring pin 4, and a locking knob 2. The knob spring pin 4 and the locking knob 2 are respectively connected to the limit mounting plate 10.

[0063] In one embodiment, see Figure 7 and Fig. 9 The above-mentioned cell factory 12 fixing structure includes a locking position adjustment plate 3 , the locking position adjustment plate 3 is connected to the carrier body 1 , and the locking knob 2 is connected to the locking position adjustment plate 3 .

[0064] In one embodiment, see Figure 7 and Fig. 9The locking position adjustment plate 3 is provided with a groove, and one end of the locking knob 2 is embedded in the groove. The fixing structure of the cell factory 12 can adjust the fixing position and is suitable for cell factories 12 with 1 to 10 layers.

[0065] Loosen the locking knob 2 and slide the locking knob 2 up and down in the groove of the locking position adjustment plate 3 to drive the limit mounting plate 10 to move up and down, so that the knob spring pin 4 presses the cell factory 12, and then tighten the locking knob 2 to fix the cell factory 12 in the carrier body 1. If the cell factory 12 is to be taken out, pull out the knob spring pin 4 and rotate the rotating spring pin to retract the rotating spring pin, thereby loosening the fixation of the cell factory 12 and taking out the cell factory 12 from the carrier body 1.

[0066] In one embodiment, see Figure 8 The above-mentioned clamping structure includes a clamping plate 11, which is installed on the outside of the carrier body 1 and connected to the manipulator.

[0067] The clamping plate 11 is arranged to cooperate with the manipulator for clamping by the manipulator, and the manipulator pushes the carrier body 1 to move along the guide rail 22 in the box body 15, so that the connecting tube 8 of the gas sealing docking structure and the ventilation interface 21 in the box body 15 are automatically plugged and connected. After the cultivation is completed, the manipulator clamps the carrier body 1 and pulls it outward to pull out the connecting tube 8 and disconnect the gas circuit.

[0068] In one embodiment, see Figure 7 and Fig. 9 The above-mentioned gas sealing docking structure includes an air guide plate 9, an air pipe limiting seat 7 and a connecting pipe 8. The connecting pipe 8 is connected to the air pipe limiting seat 7. The air pipe limiting seat 7 is provided with a through groove. The air guide plate 9 is provided with a cavity. The connecting pipe 8, the through groove and the cavity are connected.

[0069] In one embodiment, see Figure 7 and Fig. 9 The air guide plate 9 is provided with a through hole, which is communicated with the cavity, and the through hole is connected with a pagoda air pipe connector 6, which is connected with the cell factory 12 through a hose 13.

[0070] In one embodiment, see Figure 7 and Fig. 9 The connecting pipe 8 mentioned above includes a silicone hose 13 .

[0071] In this embodiment, the silicone hose 13 is fixed in the air pipe limiting seat 7 and is connected to the pagoda air pipe connector 6 through the air guide plate 9.

[0072] In one embodiment, see Fig. 9The cell factory 12 is connected to a sterile filter 14 , and the sterile filter 14 is connected to a hose 13 .

[0073] Connect the ventilation hose 13 with a sterile filter 14 between the cover of the cell factory 12 and the pagoda air pipe connector 6. After the silicone hose 13 is connected to the air path in the box 15, the cell factory 12 can be connected and sealed with the air path in the box 15, thereby achieving the control of the concentration of carbon dioxide, oxygen and water vapor in the cell factory 12.

[0074] The carrier of this embodiment is designed with guide rollers 5, guide rails 22 and gas-sealed docking structures. Under the push of the manipulator, the carrier body 1 moves along the guide rails 22 in the box 15, and the connecting pipe 8 of the gas-sealed docking structure is automatically plugged and connected with the ventilation interface 21 in the box 15. This design enables the gas circuit to be quickly connected and disconnected without the intervention of an additional power device; the gas-sealed docking structure includes an air guide plate 9, a tracheal limit seat 7 and a connecting pipe 8, and the connecting pipe 8 is connected to the tracheal limit seat 7. During the connection process, these components cooperate with each other, and the design of the gas-sealed docking structure is used to achieve the sealing of the gas circuit, avoid gas leakage, and ensure the cleanliness of the culture environment. The carrier is designed with a clamping structure, and through the cooperation of the clamping plate 11 and the manipulator, the manipulator can automatically clamp the carrier body 1 and perform mobile operations, thereby realizing fully automated operation. In addition, the fixing structure of the cell factory 12 is also carefully designed, and the cell factory 12 can be easily taken out or fixed without manual intervention, thereby reducing the potential contamination risk caused by manual operation.

[0075] In general, the design realizes the functions of unpowered gas path docking, disconnection and sealing through reasonable coordination and structural design among various components, while adapting to the fully automated operation of the manipulator, thus ensuring the efficiency, safety and pollution-free nature of the cell culture process.

[0076] The above-mentioned cell factory carrier 20, by setting the carrier body 1, the cell factory 12 fixing structure, the gas sealing docking structure and the clamping structure, the cell factory 12 fixing structure can realize the fixing and removal of the cell factory 12, the gas sealing docking structure can realize the rapid docking, disconnection and sealing of the gas path, and the clamping structure is matched with a manipulator to realize the manipulator clamping and moving the carrier body 1, realizing fully automated operation and reducing manual intervention. The entire carrier realizes unpowered gas path docking, so that the carbon dioxide gas path in the cell factory 12 can be quickly connected and disconnected, and the gas path can be sealed, avoiding the addition of an additional power device in the box 15 to pollute the culture environment; and it can be suitable for fully automated operation of the manipulator, without the need for manual participation, reducing the potential pollution risks caused by manual operation.

[0077] In this embodiment, when the door opening and closing mechanism opens the incubator door 18 into place, the robot moves the cell factory carrier 20 so that the cell factory carrier 20 moves into the box body 15 and is connected to the ventilation interface 21. When the in-situ sensor detects that the cell factory carrier 20 is in place, the carbon dioxide concentration in the cell factory is controlled by the carbon dioxide control component 27, and the incubator door 18 is closed; after the cell culture is completed, the incubator door 18 is opened and the cell factory carrier 20 is pulled out.

[0078] Specifically, the touch screen 30 can be touched to open the incubator door 18, or the incubator door 18 can be opened by remote control. The door opening motor assembly 34 starts working to open the incubator door 18. After the door opening angle sensor senses the door opening sensing plate 35, it indicates that the door is in place, and the door opening motor assembly 34 stops working. The cell factory and the cell factory carrier 20 can be placed on the guide rail 22 of the box 15 manually or automatically by a robot. The cell factory carrier 20 is pushed inward with the help of the guide rail 22 until the connecting tube 8 is inserted into the ventilation interface 21 on the carbon dioxide control assembly 27. The in-position sensor 28 senses the cell factory carrier 20, and the gas path is automatically connected and sealed. The box body 15 closes the door under the control of the door opening motor assembly 34 and the door opening angle sensor 36, and then the cell culture begins. The carbon dioxide control assembly 27 can accurately control the carbon dioxide concentration in the cell factory through the gas path and the corresponding sensor, which helps to maintain the gas environment required for cell culture and improve the culture efficiency and quality. After the cell culture is completed, the box door 18 automatically opens, and the cell factory and the cell factory carrier 20 can be pulled out along the guide rail 22, and the carbon dioxide gas path can be automatically disconnected.

[0079] In this embodiment, the positioning plate and the positioning pin 25 help to accurately position, thereby improving the accuracy and stability of automated operations; the automatic door opening and closing mechanism realizes automatic door opening and closing, thereby improving the efficiency and convenience of automated operations; the air path docking sealing structure can quickly and accurately connect and disconnect the ventilation line, thereby avoiding increasing the power device to pollute the culture environment, while controlling the carbon dioxide concentration in the cell factory; carbon dioxide concentration detection and control can ensure that the carbon dioxide concentration in the cell factory reaches the required value, thereby improving cell culture efficiency and quality; the guide rail 22 assists the operation of the manipulator, accurately guides the cell factory carrier 20, facilitates the automatic connection and disconnection of the carbon dioxide ventilation line, and reduces the potential pollution risks caused by manual operation.

[0080] The above-mentioned stem cell incubator suitable for automated cell culture is provided with an incubator body and a cell factory carrier 20. The incubator body includes a box body 15, an incubator door 18 and a door opening and closing mechanism. The door opening and closing mechanism can automatically open or close the incubator door 18. The in-situ sensor can accurately control the ventilation and concentration of carbon dioxide by the carbon dioxide control component 27 after detecting that the cell factory carrier 20 is in place. A guide rail 22 is provided to facilitate the cell factory carrier 20 to move in or out of the box body 15, thereby solving the problems of slow carbon dioxide concentration adjustment and dependence on manual operation in traditional cell culture incubators, and improving the efficiency and quality of cell culture.

[0081] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the utility model, and these modifications or replacements should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A stem cell incubator suitable for automated cell culture, characterized in that: include: An incubator body and a cell factory carrier, wherein the incubator body comprises a box body, an incubator door and a door opening and closing mechanism, an opening is provided on one side of the box body, the incubator door is connected to the opening of the box body through the door opening and closing mechanism, a carbon dioxide control component and an in-situ sensor are installed in the box body, a ventilation interface is installed on the carbon dioxide control component, a guide rail is installed in the box body, and the cell factory carrier is respectively connected to the guide rail and the ventilation interface.

2. A stem cell culture incubator suitable for automated cell culture according to claim 1, characterized in that: The box body and the door opening and closing mechanism are combined to form a cavity, a guide rail mounting plate is installed at the bottom of the cavity, and the guide rail is connected to the guide rail mounting plate.

3. A stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: The lower end of the box is connected with an adjustable supporting foot and a positioning pin.

4. A stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: The door opening and closing mechanism includes a door shaft seat, a door shaft, a door opening motor assembly, a door opening sensor plate and a door opening angle sensor. The door shaft seat is installed on the box body, the door shaft is installed on the door shaft seat, the incubator door is connected to the door opening motor assembly, the door opening sensor plate is connected to the incubator door, and the door opening angle sensor is installed on the door shaft.

5. The stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: A heat insulation strip is installed on one side of the incubator door close to the box body.

6. The stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: A sealing strip is installed at the opening of the box body.

7. A stem cell culture box suitable for automated cell culture according to claim 2, characterized in that: An air duct and a fan assembly are installed on the upper end surface of the cavity, and the fan assembly is connected to the air duct.

8. The stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: The box body is provided with an interface, a touch screen and an indication alarm light.

9. The stem cell culture box suitable for automated cell culture according to claim 1, characterized in that: The cell factory carrier includes: a carrier body, a cell factory fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body, the clamping structure is connected to a manipulator, and the gas-sealed docking structure is connected to the ventilation interface; a guide roller is installed under the carrier body, and the guide roller is in rolling contact with the guide rail.

10. A stem cell culture box suitable for automated cell culture according to claim 9, characterized in that: The cell factory fixing structure comprises a limit mounting plate, a knob spring pin, and a locking knob. The knob spring pin and the locking knob are respectively connected to the limit mounting plate.

Images