Kit for culturing primary umbilical cord mesenchymal stem cells
By designing multiple fixing plates and sliding clamping plates in the reagent kit, combined with the structure of clamping springs and extrusion plates, the problems of insecure storage and insufficient adaptability of the reagent tubes are solved, and the safe, flexible and convenient storage and access of the reagent tubes are achieved.
Patent Information
- Application Number
- CN202421439929.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-21
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2034-06-21
AI Technical Summary
The existing kits are not firm and adaptable to the internal reagent tubes when used, resulting in easy shaking of the reagent tubes and spilling out liquids, affecting safety, and can only store reagent tubes that match the size of the sponge holes, which are insufficient flexibility and adaptability.
A reagent box including a reagent storage box that is slidingly connected to the outer shell and an inner sliding connection is designed. A multiple fixing plate and a sliding clamping plate are provided in the reagent storage box. A clamping spring is provided between the clamping plate and the reagent storage box. The bottom end of the clamping plate penetrates through the bottom of the reagent storage box and is in contact with the extrusion plate to achieve sliding fixation and loosening of the clamping plate, and adapt to reagent tubes of different sizes.
Through the design of clamping plates and fixing plates, the reagent tubes are securely stored, liquid spilling and contamination are avoided, the safety and adaptability of reagent tubes are improved, and the storage and access operations of reagent tubes are simplified.
Smart Images
Figure CN222683061U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of test kits, in particular to a test kit for culturing primary umbilical cord mesenchymal stem cells. Background Art
[0002] Mesenchymal stem cells are a type of adult stem cells with high self-renewal and multidirectional differentiation potential. Under certain conditions, they can differentiate into adipocytes, chondrocytes, osteoblasts, neurons, cardiomyocytes, etc. Umbilical cord mesenchymal stem cells are isolated from different parts of the umbilical cord, such as the venous endothelium, subendothelial layer, Wharton's jelly, blood vessels and surrounding tissues. Umbilical cord mesenchymal stem cells have many advantages: rich sources, easy collection, and no social, ethical and legal disputes; large numbers, strong proliferation ability, can be used multiple times; genetic stability, not easy to mutate, safe and reliable to use; umbilical cord mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, neurons, cardiomyocytes, etc. Cells, fat cells, etc., have good plasticity; low immunogenicity, have immunoregulatory function, do not cause immune rejection reaction when used, and can also inhibit the reaction, etc. In the process of culturing umbilical cord mesenchymal stem cells, a reagent kit is usually required, and when the existing reagent tube is placed inside the reagent kit, on the one hand, the reagent tube is only limited by the sponge pad, which makes the reagent tube easy to shake during movement, and then the internal liquid is easy to spill and cause pollution, affecting the safety of the reagent tube storage, on the other hand, a reagent kit can only store reagent tubes that match the size of the sponge opening, resulting in insufficient flexibility and adaptability of the existing reagent kit when used. Utility Model Content
[0003] The utility model aims to provide a reagent kit for culturing primary umbilical cord mesenchymal stem cells, which can solve the technical problems of insufficient firmness and adaptability of internal reagent tubes in the existing reagent kit when in use, effectively fix reagent tubes of different sizes, avoid shaking of the reagent tubes during movement, ensure the safety of reagent tube storage, and greatly improve the adaptability of placing reagent tubes of different sizes.
[0004] In order to achieve the above purpose, the utility model is implemented through the following technical solutions:
[0005] A kit for culturing primary umbilical cord mesenchymal stem cells comprises an outer shell, a reagent storage box for placing reagent tubes being slidably connected therein, a plurality of fixed plates being provided in the reagent storage box, a plurality of clamping plates being symmetrically slidably connected therein along the transverse direction, the reagent tubes being located between the clamping plates and the fixed plates, a clamping spring being provided between the clamping plates and the reagent storage box, the bottom end of the clamping plates penetrating the bottom of the reagent storage box and then extending downward, an extrusion plate for driving the clamping plates to slide toward the fixed plates being provided at the bottom of the outer shell, and both sides of the extrusion plate being in sliding contact with the clamping plates on both sides respectively.
[0006] Furthermore, the front side of the extrusion plate is in a pointed cone shape.
[0007] Furthermore, a tool box is slidably connected in the outer shell, and a fixed partition and a rotating partition are arranged in the tool box. A culture dish is arranged on the fixed partition, and a scissors are arranged on the rotating partition.
[0008] Furthermore, the rotating partition is rotatably connected to the tool box, a spring sheet for driving the rotating partition to rotate is provided at the bottom of the tool box, a pressure rod is provided at the top of the rotating partition, and the pressure rod is in sliding contact with the top of the outer shell.
[0009] Furthermore, a fixing frame is vertically slidably connected to the rotating partition, a compression spring is provided between the fixing frame and the rotating partition, and the scissors are located between the top of the fixing frame and the rotating partition.
[0010] Furthermore, a buffer sponge and a buffer plate used in conjunction with the reagent storage box are respectively provided on the top and bottom of the outer shell.
[0011] Compared with the prior art, the beneficial effects of the present invention are:
[0012] 1. The structure of the utility model is that a reagent storage box is slidably arranged in the outer shell, and a plurality of fixed plates and sliding clamping plates are arranged in the reagent storage box. A clamping spring is arranged between the clamping plate and the reagent storage box. Such a structure clamps and fixes the reagent tube between the clamping plate and the fixed plate, thereby ensuring the safety of the reagent tube storage, avoiding the possible sliding of the reagent tube during the movement of the outer shell, avoiding the situation where the liquid inside the reagent tube spills out and causes pollution, ensuring the safety of the reagent tube storage, and reagent tubes of different sizes can be clamped and fixed between the fixed plate and the clamping plate under the action of the clamping plate, which greatly improves the adaptability to the placement of reagent tubes of different sizes;
[0013] 2. The bottom end of the clamping plate passes through the bottom of the reagent storage box and then extends downward. The bottom of the outer shell is provided with an extrusion plate that drives the clamping plate to slide toward the fixed plate. The two sides of the extrusion plate are respectively in sliding contact with the clamping plates on both sides. With this structure, when the reagent storage box is slid into the interior of the outer shell, the clamping plates at different positions gradually contact the extrusion plate so that the clamping plate slides toward the fixed plate to fix the reagent tube. When the reagent tube is taken out, the reagent storage box is slid out so that the clamping plates at different positions are gradually separated from the extrusion plate. As a result, under the action of the clamping spring, the clamping plate is reset and slid in the direction away from the fixed plate to loosen the reagent tube for easy access to the reagent tube. Such a structure makes the storage and retrieval of the reagent tube simpler, greatly improving the firmness of the storage of the reagent tube and the convenience of taking. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Attached Figure 1 It is a three-dimensional structural schematic diagram of the utility model.
[0015] Attached Figure 2 It is a front view of the utility model.
[0016] Attached Figure 3 It is the attachment of the utility model Figure 2 Cross-sectional view along the AA direction.
[0017] Attached Figure 4 It is the attachment of the utility model Figure 3 Cross-sectional view along the BB direction.
[0018] Attached Figure 5 It is the attachment of the utility model Figure 4 Cross-sectional view along CC direction.
[0019] Attached Figure 6 It is the attachment of the utility model Figure 4 Cross-sectional view along the DD direction.
[0020] Numbers shown in the accompanying drawings:
[0021] 1. Outer shell; 2. Reagent tube; 3. Reagent storage box; 4. Fixed plate; 5. Clamping plate; 6. Clamping spring; 7. Squeeze plate; 8. Tool box; 9. Fixed partition; 10. Rotating partition; 11. Culture dish; 12. Scissors; 13. Spring sheet; 14. Pressure rod; 15. Fixed frame; 16. Compression spring; 17. Buffer sponge; 18. Buffer plate. DETAILED DESCRIPTION
[0022] The present invention is further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the present application.
[0023] Reference Figure 1 and Figure 2The utility model is a kit for culturing primary umbilical cord mesenchymal stem cells. The main structure includes an outer shell 1, an inlet is opened on the front side of the outer shell 1, and a reagent storage box 3 for placing a reagent tube 2 is slidably connected in the outer shell 1. The reagent storage box 3 slides into the interior of the outer shell 1 after passing through the inlet on the front side. The reagent storage box 3 is a box structure with an open top, and the reagent tube 2 is stored and retrieved from the open top. A handle is provided on the front side of the reagent storage box 3 to facilitate the sliding control of the reagent storage box 3. A plurality of fixing plates 4 are welded or integrally formed and fixed in the reagent storage box 3. Preferably, the cross section of the fixing plate 4 is V-shaped, so that the curved side of the reagent tube 2 can be better clamped and fixed. A plurality of clamping plates 5 are symmetrically connected in the transverse sliding manner in the reagent storage box 3. Specifically, a through hole is set in the reagent storage box 3, and the clamping plate 5 is slidably connected in the through hole. Figure 5 The clamping plate 5 is preferably symmetrically rotated on the side of the clamping plate 5, and a torsion spring is provided between the elastic clamping plate 5 and the clamping plate 5, so that the elastic clamping plates on both sides can more closely contact and clamp the arc-shaped sides of reagent tubes 2 of different sizes. The reagent tube 2 is located between the clamping plate 5 and the fixing plate 4. When the clamping plate 5 slides toward the fixing plate 4, the reagent tube 2 is clamped on the fixing plate 4, so that the reagent tube 2 is more firmly stored. When the outer shell 1 moves, the reagent tube 2 is not easy to shake, which avoids the pollution caused by the spillage of the internal liquid and improves the safety of the storage of the reagent tube 2. A clamping spring 6 is provided between the clamping plate 5 and the reagent storage box 3. The elastic force generated by the clamping spring 6 enables the clamping plate 5 to maintain a certain clamping effect after sliding different distances, thereby meeting the clamping requirements when placing reagent tubes 2 of different sizes and improving the adaptability of placing reagent tubes 2 of different sizes. The bottom end of the clamping plate 5 passes through the bottom of the reagent storage box 3 and moves backward. When the reagent storage box 3 is taken out, the clamping plates 5 at different positions are gradually separated from the squeezing plates 7. Under the action of the clamping spring 6, the clamping plates 5 are reset and slid in the direction away from the fixed plate 4 to release the clamping plate 5 from the reagent tube 2, so that the clamping plate 5 is automatically released when the reagent storage box 3 is taken out, which is convenient for taking the reagent tube 2, and greatly improves the stability of the storage of the reagent tube 2 and the convenience of taking.
[0024] Preferably, refer to Figure 6The front side of the squeezing plate 7 is a pointed cone. Such a structure enables the front side of the pointed cone-shaped squeezing plate 7 to enter between the two clamping plates 5 more smoothly when the reagent storage box 3 is slid into the outer shell 1, and then gradually squeeze the clamping plates 5 on both sides toward both sides, thereby achieving the clamping and fixing of the reagent tube 2 by the clamping plates 5 on both sides, and improving the smoothness of the clamping and fixing of the reagent tube 2 by the clamping plates 5 when the reagent storage box 3 is slid for storage.
[0025] Preferably, refer to Figure 3 A tool box 8 is also slidably connected to the outer shell 1. The tool box 8 is used to place tools used in the culture process. The top of the tool box 8 is open, which is convenient for storing and accessing the tools from the open. A fixed partition 9 and a rotating partition 10 rotatably connected by a pin or a hinge are welded or integrally formed in the tool box 8. A culture dish 11 is provided on the fixed partition 9, and a scissors 12 are provided on the rotating partition 10. In order to prevent the scissors 12 from contacting the blade of the scissors 12 when taking the scissors 12, the scissors 12 are placed on the rotating partition 10, so that the rotating partition 10 automatically rotates when taking the scissors 12, so that the handle of the scissors 12 is flipped upward, so that the handle can be directly taken, further improving the safety of the scissors 12 when taking.
[0026] Preferably, the rotating partition 10 is rotatably connected to the tool box 8 via a pin or a hinge. A spring sheet 13 for driving the rotating partition 10 to rotate is provided at the bottom of the tool box 8. The spring sheet 13 is preferably L-shaped and contacts the bottom of the rotating partition 10, so that the rotating partition 10 has a tendency to flip upward. An inclined pressure rod 14 is welded or integrally formed on the top of the rotating partition 10, and the pressure rod 14 is in sliding contact with the top of the outer shell 1. Such a structure enables the inclined pressure rod 14 to slide and be stored into the outer shell 1 when the tool box 8 is slid. The rod 14 gradually slides into contact with the top of the outer shell 1, thereby pressing the rotating partition 10 at the bottom of the pressure rod 14 downward, so that the rotating partition 10 flips downward and the scissors 12 are placed flat on the rotating partition 10. When taking the tool box 8, the pressure rod 14 on the top of the rotating partition 10 is gradually separated from the top of the outer shell 1. Under the action of the bottom spring sheet 13, the rotating partition 10 automatically flips upward, so that the handle of the scissors 12 on the rotating partition 10 is rotated to a high position, which is convenient for taking the scissors 12, making it more convenient and safe to take the scissors 12.
[0027] Preferably, refer to Figure 4A fixing frame 15 is vertically slidably connected to the rotating partition 10. The longitudinal section of the fixing frame 15 is an inverted U-shape, which passes through the rotating partition 10 and is slidably connected thereto. A compression spring 16 is provided between the bottom of the inverted U-shape of the fixing frame 15 and the rotating partition 10. The scissors 12 are located between the top of the fixing frame 15 and the rotating partition 10. When the scissors 12 are placed, they are gradually inserted into the area between the top of the fixing frame 15 and the rotating partition 10. In this process, the fixing frame 15 is driven to slide upward to compress the compression spring 16. The reverse elastic force generated by the compression spring 16 is used to press the blade end of the scissors 12 against the rotating partition 10, thereby avoiding the shaking of the scissors 12 when the outer shell 1 moves, thereby improving the firmness and safety of the placement of the scissors 12.
[0028] Preferably, the top and bottom of the outer shell 1 are respectively provided with a buffer sponge 17 and a buffer plate 18 for use with the reagent storage box 3. Specifically, the buffer sponge 17 is located at the top of the reagent tube 2, and a plurality of buffer springs and matching dampers are provided between the bottom of the buffer plate 18 and the bottom of the outer shell 1, so that when the outer shell 1 falls or bumps, the buffer sponge 17 and the buffer plate 18 buffer the impact force, thereby preventing the reagent tube 2 in the reagent storage box 3 from being damaged by collision due to the impact force, further ensuring the safety of the storage of the reagent tube 2, and a suction cup can also be provided at the bottom of the outside of the outer shell 1, so that the outer shell 1 is adsorbed on the operating table when it is placed, reducing the possibility of damage caused by falling of the outer shell 1.
[0029] Working principle: The structure of the utility model is that a reagent storage box 3 is slidably arranged in the outer shell 1, and a plurality of fixed plates 4 and sliding clamping plates 5 are arranged in the reagent storage box 3. A clamping spring 6 is arranged between the clamping plate 5 and the reagent storage box 3. Such a structure clamps and fixes the reagent tube 2 between the clamping plate 5 and the fixed plate 4, thereby ensuring the safety of the storage of the reagent tube 2, avoiding the possible sliding of the reagent tube 2 during the movement of the outer shell 1, and avoiding the situation where the liquid inside the reagent tube 2 is spilled and causes pollution, thereby ensuring the safety of the storage of the reagent tube 2, and reagent tubes 2 of different sizes can be clamped and fixed between the fixed plate 4 and the clamping plate 5 under the action of the clamping plate 5, which greatly improves the adaptability to the placement of reagent tubes 2 of different sizes; the bottom end of the clamping plate 5 penetrates the bottom of the reagent storage box 3 and then extends downward, and the outer The bottom of the shell 1 is provided with an extrusion plate 7 that drives the clamping plate 5 to slide toward the fixed plate 4, and the two sides of the extrusion plate 7 are in sliding contact with the clamping plates 5 on both sides respectively. With such a structure, when the reagent storage box 3 is slid into the outer shell 1, the clamping plates 5 at different positions gradually contact the extrusion plate 7 so that the clamping plate 5 slides toward the fixed plate 4 to fix the reagent tube 2. When the reagent tube 2 is taken out, the reagent storage box 3 is slid and pulled out, so that the clamping plates 5 at different positions are gradually separated from the extrusion plate 7, so that under the action of the clamping spring 6, the clamping plate 5 is reset and slid in the direction away from the fixed plate 4, and the reagent tube 2 is released, which is convenient for taking the reagent tube 2. Such a structure makes the storage and taking operations of the reagent tube 2 simpler, and greatly improves the storage firmness and the convenience of taking the reagent tube 2.
Claims
1. A kit for culturing primary umbilical cord mesenchymal stem cells, comprising an outer shell (1), wherein a reagent storage box (3) for placing a reagent tube (2) is slidably connected to the outer shell (1), characterized in that: The reagent storage box (3) is provided with a plurality of fixed plates (4), and the reagent storage box (3) is symmetrically connected with a plurality of clamping plates (5) in a lateral sliding manner. The reagent tube (2) is located between the clamping plate (5) and the fixed plate (4). A clamping spring (6) is provided between the clamping plate (5) and the reagent storage box (3). The bottom end of the clamping plate (5) passes through the bottom of the reagent storage box (3) and then extends downward. The bottom of the outer shell (1) is provided with an extrusion plate (7) for driving the clamping plate (5) to slide toward the fixed plate (4), and the two sides of the extrusion plate (7) are respectively in sliding contact with the clamping plates (5) on both sides.
2. A kit for culturing primary umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The front side of the extrusion plate (7) is in a pointed cone shape.
3. A kit for culturing primary umbilical cord mesenchymal stem cells according to claim 1, characterized in that: A tool box (8) is also slidably connected to the outer shell (1), and a fixed partition (9) and a rotating partition (10) are provided in the tool box (8). A culture dish (11) is provided on the fixed partition (9), and a pair of scissors (12) is provided on the rotating partition (10).
4. A kit for culturing primary umbilical cord mesenchymal stem cells according to claim 3, characterized in that: The rotating partition (10) is rotatably connected to the tool box (8); a spring sheet (13) for driving the rotating partition (10) to rotate is provided at the bottom of the tool box (8); a pressure rod (14) is provided at the top of the rotating partition (10); and the pressure rod (14) is in sliding contact with the top of the outer shell (1).
5. A kit for culturing primary umbilical cord mesenchymal stem cells according to claim 4, characterized in that: A fixing frame (15) is vertically slidably connected to the rotating partition (10), a compression spring (16) is provided between the fixing frame (15) and the rotating partition (10), and the scissors (12) are located between the top of the fixing frame (15) and the rotating partition (10).
6. A kit for culturing primary umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The top and bottom of the outer shell (1) are respectively provided with a buffer sponge (17) and a buffer plate (18) used in conjunction with the reagent storage box (3).