Adapter of automatic sampling machine and automatic sampling machine

Abstract

The utility model discloses an adapter of an automatic sampling machine and the automatic sampling machine. The adapter comprises an adapter main body, wherein a placing groove is defined by the adapter main body; the cover body covers the open end of the placing groove, a liquid injection hole is formed in the cover body, the liquid injection hole is communicated with the placing groove, and a liquid drainage hole is formed in at least one of the adapter main body and the cover body. Through cooperation of the adapter main body and the cover body, a to-be-detected cell or tissue sample is fixed on the sample bearing part, at least part of the sample bearing part is placed in the placing groove, the to-be-detected cell or tissue sample is located in the placing groove, and corresponding liquid reagents can be injected into the placing groove through the plurality of liquid injection holes. The liquid reagent can be automatically injected into the containing groove, experimenters do not need to be on the spot, labor force is greatly saved, the influence of repeated labor on the physical and psychological health of the experimenters is reduced, the liquid reagent in the containing groove can be discharged out of the containing groove through the liquid discharging hole, and waste liquid in the containing groove can be discharged out of the containing groove.

Description

Technical Field

[0001] The utility model relates to the field of immunofluorescence imaging, in particular to an adapter of an automatic sample making machine and a corresponding automatic sample making machine. Background Art

[0002] In the related technology, immunofluorescence imaging is a commonly used molecular biology technique for detecting and visualizing the location and expression of specific antigens or proteins in cells and tissues. Combining immunology and fluorescence microscopy technology, it can provide high-resolution images of cells or tissues. Immunofluorescence imaging contains a process of labeling samples with fluorescent antibodies when making samples. At present, the preparation of samples is mainly done by manpower, and it usually takes experimenters at least more than 3 hours to prepare samples. During the sample preparation period, the experimenters need to stay near the samples and operate from time to time, resulting in a serious waste of labor. This long-term mechanical repetitive labor also has a huge impact on the physical and mental health of the experimenters. Utility Model Content

[0003] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, one purpose of the utility model is to propose an adapter for an automatic sample preparation machine, which is conducive to automatically injecting liquid reagents into the sample area when preparing samples, without the need for the presence of experimenters, greatly saving labor and reducing the impact of repeated labor on the physical and mental health of experimenters.

[0004] The utility model further proposes an automatic sample making machine.

[0005] The adapter of the automatic sample making machine according to the utility model comprises:

[0006] An adapter body, wherein the adapter body defines a placement slot with one end open, and the placement slot is used to place the sample carrier;

[0007] A cover body is provided on the open end of the placement groove, and the cover body is formed with a plurality of liquid injection holes, and the plurality of liquid injection holes are all connected with the placement groove, and at least one of the adapter body and the cover body is formed with a drainage hole connected with the placement groove.

[0008] According to the adapter of the automatic sample preparation machine of the utility model, the cell or tissue sample to be detected is fixed on the sample carrier through the cooperation of the adapter body and the cover body, and at least a part of the sample carrier is placed in the placement groove, so that the cell or tissue sample to be detected is located in the placement groove, and corresponding liquid reagents can be injected into the placement groove respectively through multiple liquid injection holes, which is conducive to automatically injecting liquid reagents into the placement groove without the need for experimenters to be present, greatly saving labor and reducing the impact of repetitive labor on the physical and mental health of experimenters. In addition, by setting the drainage hole, the liquid reagent in the placement groove can be discharged out of the placement groove, which is conducive to discharging the waste liquid in the placement groove.

[0009] In some examples of the present invention, the cover body includes a first main body, which is covered at an open end of the placement groove and is formed with a plurality of the liquid injection holes.

[0010] In some examples of the present invention, the cover body also includes a second main body, which is overlapped with the first main body and is located on the side of the first main body facing the adapter main body, and the adapter main body is formed with an assembly hole opposite to and connected to the open end of the placement slot, and the assembly hole is located on the side of the open end of the placement slot facing the cover body, and when the cover body is covered on the open end of the placement slot, the second main body is assembled in the assembly hole and abuts against the inner peripheral wall of the assembly hole.

[0011] In some examples of the present invention, the second body is annular, and along the stacking direction of the second body and the first body, the orthographic projections of the plurality of liquid injection holes are all located within the orthographic projection range of the second body.

[0012] In some examples of the present invention, the adapter body is formed with the drainage hole, and the drainage hole extends to the bottom wall surface of the placement groove.

[0013] In some examples of the present invention, the adapter of the automatic sample preparation machine also includes: a ring, which defines a first through hole, and the ring is arranged in the placement groove, and the ring is suitable for overlapping with the sample carrier to form a sample groove in the placement groove, and the sample groove is suitable for placing samples, and the drainage hole and the multiple liquid injection holes are all connected to the sample groove.

[0014] In some examples of the present invention, the open end of the sample slot is opposite to the liquid drainage hole and the plurality of liquid injection holes.

[0015] In some examples of the present invention, the adapter of the automatic sample preparation machine further includes: a sealing ring, which is suitable for being assembled between the end of the ring facing away from the cover body and the sample carrier, and the sealing ring is arranged around the first through hole.

[0016] In some examples of the present invention, the adapter of the automatic sample preparation machine also includes: a waste liquid drain pipe, the cover body is formed with the drain hole, the waste liquid drain pipe is passed through the drain hole and one end extends into the sample tank, and the waste liquid drain pipe abuts against the bottom wall of the sample tank.

[0017] In some examples of the present invention, the cover body has a cover body end wall, the cover body end wall is formed with the liquid injection hole and the liquid discharge hole, and along the depth direction of the placement groove, the cover body end wall is opposite to the bottom wall of the placement groove;

[0018] An assembly groove is formed on the outer surface of the end wall of the cover body, the liquid discharge hole is located at the assembly groove, and at least a part of the waste liquid discharge pipe is assembled in the assembly groove.

[0019] In some examples of the present invention, the adapter of the automatic sample making machine also includes: an elastic pressing sheet, which is located on the side of the waste liquid discharge pipe away from the end wall of the cover body, and one end of the elastic pressing sheet is fixed to the end wall of the cover body, and the elastic pressing sheet is pressed against the waste liquid discharge pipe.

[0020] In some examples of the utility model, the cover body also has an annular cover body side wall, which is arranged around the circumferential edge of the cover body end wall, and the outer surface of the cover body side wall is formed with a first threaded portion, and the inner wall of the placement groove is formed with a second threaded portion, and the second threaded portion is adjacent to the open end of the placement groove, and the first threaded portion and the second threaded portion are assembled together to fix the cover body and the adapter body.

[0021] In some examples of the present invention, a second through hole communicating with the placement groove is formed on a side wall of the placement groove, and the sample carrier is assembled in the placement groove through the second through hole.

[0022] The automatic sample making machine according to the utility model comprises:

[0023] An adapter, wherein the adapter is the above-mentioned adapter;

[0024] A first peristaltic pump and a plurality of syringes, each of the syringes and the first peristaltic pump is connected with an electromagnetic valve, and the syringe outlets of the plurality of syringes are adapted to communicate with the placement slot;

[0025] a second peristaltic pump and a liquid storage bottle, wherein the second peristaltic pump is connected between the liquid storage bottle and the liquid discharge hole;

[0026] A controller is configured to control the first peristaltic pump and the second peristaltic pump to operate, and is also configured to control the electromagnetic valve to open or close.

[0027] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0029] Figure 1 is a schematic diagram of an adapter according to a first embodiment of the utility model;

[0030] Figure 2 is an exploded view of an adapter according to a first embodiment of the utility model;

[0031] Figure 3 is a bottom view of the cover of the adapter according to the first embodiment of the utility model;

[0032] Figure 4 is a cross-sectional view of a cover of an adapter according to a first embodiment of the utility model;

[0033] Figure 5 is a cross-sectional view of an adapter body of an adapter according to a first embodiment of the utility model;

[0034] Figure 6 is a schematic diagram of an adapter according to a second embodiment of the utility model;

[0035] Figure 7 is an exploded view of an adapter according to a second embodiment of the utility model;

[0036] Figure 8 is a cross-sectional view of a collar according to an embodiment of the utility model;

[0037] Fig. 9 is a schematic diagram of an adapter according to a third embodiment of the utility model;

[0038] Fig.10 is an exploded view of an adapter according to a third embodiment of the utility model;

[0039] Fig.11 It is a principle diagram of an automatic sample making machine according to an embodiment of the utility model.

[0040] Reference numerals:

[0041] 100. Adapter;

[0042] 10. adapter body; 11. placement groove; 111. bottom wall surface; 112. second threaded portion; 113. second through hole; 12. drainage hole; 13. connecting joint; 14. assembly hole; 15. observation hole;

[0043] 20. Cover body; 21. Liquid injection hole; 22. First body; 23. Second body;

[0044] 201, first peristaltic pump; 202, syringe; 203, electromagnetic valve; 204, second peristaltic pump; 205, liquid storage bottle;

[0045] 206. Controller;

[0046] 31. collar; 311. first through hole; 32. sealing ring; 33. stop ring; 34. waste liquid discharge pipe; 35. cover end wall; 351. assembly groove; 36. elastic pressing piece; 37. cover side wall; 371. first threaded portion; 38. elastic mounting groove;

[0047] 200, sample carrier; 2001, confocal dish; 2011, bottom wall of the dish; 2012, side wall of the dish. DETAILED DESCRIPTION

[0048] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0049] Reference below Figure 1-Figure 11 The adapter 100 of the automatic sample making machine according to the embodiment of the utility model is described, and the adapter 100 is a component of the automatic sample making machine. The automatic sample making machine may also include a first peristaltic pump 201, a second peristaltic pump 204, a liquid storage bottle 205, a controller 206 and a plurality of syringes 202. The adapter 100 may be a 12 mm adapter 100.

[0050] like Figure 1-Figure 11 As shown, the adapter 100 according to the embodiment of the utility model comprises: an adapter body 10 and a cover body 20. The adapter body 10 defines a placement groove 11 with one end open, and the placement groove 11 is used to place a sample carrier 200, and the cell or tissue sample to be detected is placed on the sample carrier 200. The cover body 20 is covered at the open end of the placement groove 11, and the cover body 20 is formed with a plurality of liquid injection holes 21, and the plurality of liquid injection holes 21 are all connected to the placement groove 11, and at least one of the adapter body 10 and the cover body 20 is formed with a liquid discharge hole connected to the placement groove 11.

[0051] Wherein, the adapter body 10 can be made of stainless steel, the cross section of the placement groove 11 can be circular, the cross section diameter of the placement groove 11 can be 16 mm, and the depth of the placement groove 11 can be 18 mm. The adapter body 10 and / or the cover body 20 are formed with a drainage hole 12 connected to the placement groove 11, that is, the adapter body 10 or the cover body 20 are formed with the drainage hole 12, or the adapter body 10 and the cover body 20 are both formed with the drainage hole 12. The drainage hole 12 is connected to the placement groove 11, and the liquid reagent in the placement groove 11 can be discharged from the placement groove 11 through the drainage hole 12. It should be noted that the liquid reagent in the placement groove 11 can flow out directly from the drainage hole 12, or the drainage tube is inserted into the placement groove 11 from the drainage hole 12, and the liquid reagent in the placement groove 11 is extracted out of the placement groove 11 through the drainage tube, thereby achieving the effect of discharging the liquid reagent in the placement groove 11 from the placement groove 11 through the drainage hole 12. The cover 20 can be made of aluminum alloy. When the adapter body 10 and the cover 20 are assembled, the cover 20 covers the open end of the placement groove 11, which can reduce the evaporation of water in the placement groove 11 and also protect against light. The multiple liquid injection holes 21 on the cover 20 are all connected to the placement groove 11. The cross section of the liquid injection hole 21 can be circular, and the cross-sectional diameter of the liquid injection hole 21 can be 0.8 mm. The number of liquid injection holes 21 can be two, three, four, five, six, etc. The number of liquid injection holes 21 can be reasonably designed according to actual conditions.

[0052] Among them, the process of making samples includes the following steps:

[0053] Step 1: Sample preparation. Prepare the cell or tissue sample to be tested and fix it on the sample carrier. An appropriate fixative such as formaldehyde can be used to fix the sample and maintain the morphological structure and cell membrane integrity of the sample.

[0054] Step 2: Wash: Wash with PBS several times to remove excess fixative.

[0055] Step 3: Blocking: Use blocking agents, such as bovine serum albumin (BSA) or fish skin gelatin, to block nonspecific binding sites and reduce false positive signals.

[0056] Step 4: Primary antibody incubation: Use a specific labeled probe, such as a DNA probe or an antibody probe, to bind to the target molecule to be detected.

[0057] Step 5: Washing. The sample is washed several times with a washing solution (usually a blocking solution containing a surfactant) to remove excess antibodies that are not bound to the target.

[0058] Step 6: Fluorescent secondary antibody incubation. Fluorescently conjugated secondary antibody binds to primary antibody.

[0059] Step 7: Washing. Wash the sample several times with the washing solution to remove the excess secondary antibody that has not bound to the primary antibody.

[0060] Step 8: Imaging: The sample labeled with fluorescent antibodies is imaged in a microscope imaging system.

[0061] It should be noted that the production process of step 1 may be different due to different samples. The production process of immunofluorescence from step 2 to step 7 is basically the same, and it usually takes the experimenter about 3 hours to prepare the sample. During the sample preparation, the experimenter needs to stay near the sample and operate from time to time, resulting in a serious waste of labor. This long-term mechanical repetitive labor also has a huge negative impact on the physical and mental health of the experimenter. Liquid reagents can include 6. cleaning solution, blocking solution, primary antibody solution, secondary antibody solution and PBS solution.

[0062] In the present application, during the sample preparation process, at least part of the sample carrier 200 carrying the sample is placed in the placement groove 11, so that the sample is located in the placement groove 11, and the cover body 20 is covered on the open end of the placement groove 11. After the liquid reagent required for sample preparation is loaded into a plurality of syringes 202 of the automatic sample preparation machine, the needles of the plurality of syringes 202 are respectively inserted into the plurality of liquid injection holes 21. The automatic sample preparation machine can respectively inject the corresponding liquid reagent into the placement groove 11 through the plurality of liquid injection holes 21. According to the set program and the debugged parameters, the automated reagent injection from blocking to fluorescent antibody binding can be realized, which is conducive to the automatic injection of the sample into the sample. Liquid reagents are injected into the placement groove 11 to complete the preparation of the sample. By setting the adapter 100, it is beneficial to realize automatic sample preparation. During the sample preparation process, there is no need for the presence of experimenters, which greatly saves labor and reduces the impact of repeated labor on the physical and mental health of experimenters. In addition, by setting the drainage hole 12, the drainage hole 12 can be connected to the second peristaltic pump 204. When the liquid in the placement groove 11 needs to be discharged, the second peristaltic pump 204 works and can extract the liquid reagent in the placement groove 11 through the drainage hole 12, thereby discharging the liquid reagent in the placement groove 11 out of the placement groove 11, which is beneficial to discharge the waste liquid in the placement groove 11. When it is not necessary to discharge the liquid in the placement groove 11, the second peristaltic pump 204 does not work.

[0063] According to the adapter 100 of the automatic sample preparation machine of the utility model, the cell or tissue sample to be detected is fixed on the sample carrier 200 through the cooperation of the adapter body 10 and the cover body 20, and the sample carrier 200 is placed in the placement groove 11, so that the cell or tissue sample to be detected is located in the placement groove 11, and the corresponding liquid reagents are respectively injected into the placement groove 11 through multiple liquid injection holes 21, which is conducive to automatically injecting liquid reagents into the placement groove 11 without the need for experimenters to be present, greatly saving labor and reducing the impact of repetitive labor on the physical and mental health of experimenters. In addition, by setting the drainage hole 12, the liquid reagent in the placement groove 11 can be discharged from the placement groove 11, which is conducive to discharging the waste liquid in the placement groove 11.

[0064] In some embodiments of the present invention, Figure 5 As shown, the adapter body 10 is formed with a drainage hole 12, and the drainage hole 12 extends to the bottom wall surface 111 of the placement groove 11. The cross section of the drainage hole 12 can be square, circular, etc. This application takes the cross section of the drainage hole 12 as a rectangular as an example for explanation. The long side of the cross section of the drainage hole 12 can be 0.8 mm, and the short side of the cross section of the drainage hole 12 can be 0.4 mm. The extension of the drainage hole 12 to the bottom wall surface 111 of the placement groove 11 facilitates the discharge of waste liquid in the placement groove 11 from the drainage hole 12, thereby reducing the risk of waste liquid being stored in the placement groove 11.

[0065] In some embodiments of the present invention, Figure 1 As shown, the drain hole 12 may be provided with a connecting joint 13. The connecting joint 13 may be formed with a connecting channel, which runs through the connecting joint 13. The cross section of the connecting channel may be circular, and the cross section diameter of the connecting channel may be 0.8 mm. Part of the structure of the connecting joint 13 is assembled in the drain hole 12. After the waste liquid in the placement tank 11 flows into the drain hole 12, the waste liquid in the drain hole 12 is discharged through the connecting channel. One end of the connecting joint 13 may be assembled in the drain hole 12, and the other end of the connecting joint 13 may be connected to the second peristaltic pump 204 through a latex tube. For example, one end of the latex tube is sleeved on the connecting joint 13, and the other end of the latex tube is connected to the second peristaltic pump 204. When the second peristaltic pump 204 is working, the waste liquid in the placement tank 11 can be sucked away from the drain hole 12, the connecting channel, and the latex tube.

[0066] In some embodiments of the present invention, Figure 2-Figure 4As shown, the cover body 20 may include a first main body 22, which is covered at the open end of the placement groove 11 and is formed with a plurality of liquid injection holes 21. Further, the plurality of liquid injection holes 21 may be arranged along the circumference of the first main body 22. Among them, the first main body 22 may be a plate-like structure, and the first main body 22 may be a cylindrical structure, for example: the cross-sectional diameter of the first main body 22 may be 40 mm, and the thickness of the first main body 22 may be 5 mm. The plurality of liquid injection holes 21 may be arranged in sequence along the circumference of the first main body 22. By setting the plurality of liquid injection holes 21 on the first main body 22, when the cover body 20 is covered at the open end of the placement groove 11, it is convenient for the liquid injection hole 21 to communicate with the placement groove 11, so that the liquid injection hole 21 is arranged in a reasonable position. In addition, by arranging the plurality of liquid injection holes 21 along the circumference of the first main body 22, it is convenient for the needle of the syringe 202 to be smoothly inserted into the liquid injection hole 21.

[0067] In some embodiments of the present invention, Figure 2-Figure 4 As shown, the cover body 20 may further include a second main body 23, which is stacked with the first main body 22 and located on the side of the first main body 22 facing the adapter main body 10. The adapter main body 10 is formed with an assembly hole 14 which is opposite to and connected to the open end of the placement groove 11. The assembly hole 14 is located on the side of the open end of the placement groove 11 facing the cover body 20. When the cover body 20 is covered on the open end of the placement groove 11, the second main body 23 is assembled in the assembly hole 14 and abuts against the inner peripheral wall of the assembly hole 14.

[0068] The shape of the second body 23 can be adapted to the shape of the assembly hole 14. The second body 23 can be a cylindrical structure. The cross-sectional diameter of the second body 23 is smaller than the cross-sectional diameter of the first body 22. For example, the cross-sectional diameter of the second body 23 can be 15.9 mm, and the thickness of the first body 22 can be 5 mm. The cross-sectional diameter of the second body 23 and the thickness of the first body 22 can be reasonably selected and designed according to actual needs. The second body 23 and the first body 22 are stacked and arranged. The second body 23 and the first body 22 can be integrally formed to reduce the risk of separation of the second body 23 and the first body 22. The cross-sectional area of ​​the assembly hole 14 is larger than the cross-sectional area of ​​the placement groove 11. The assembly hole 14 and the open end of the placement groove 11 are adjacent and connected. Along the depth direction of the placement groove 11, the assembly hole 14 is located on the side of the open end of the placement groove 11 facing the cover body 20. When the cover body 20 is covered on the open end of the placement groove 11, the second main body 23 is located on the side of the first main body 22 facing the adapter main body 10, and the second main body 23 is assembled in the assembly hole 14 and abuts against the inner circumferential wall of the assembly hole 14. During the assembly of the second main body 23 in the assembly hole 14, the cover body 20 and the adapter main body 10 can be aligned and assembled through the abutment and cooperation between the second main body 23 and the inner circumferential wall of the assembly hole 14, which can improve the assembly efficiency of the cover body 20 and the adapter main body 10. In addition, when the second main body 23 is assembled in the assembly hole 14, it can better reduce the evaporation of water in the placement groove 11 and can also better protect against light.

[0069] In some embodiments of the present invention, a plurality of liquid injection holes 21 are opposite to the open end of the placement slot 11. After the cover 20 is assembled to the adapter body 10, the plurality of liquid injection holes 21 are opposite to the open end of the placement slot 11, and when the needle of the syringe 202 is inserted into the liquid injection hole 21, the liquid reagent can be accurately injected into the sample area of ​​the placement slot 11.

[0070] In some embodiments of the present invention, Figure 3 As shown, the second body 23 is annular, and along the superposition direction of the second body 23 and the first body 22, the orthographic projections of the plurality of liquid injection holes 21 are all located within the orthographic projection range of the second body 23. This arrangement can prevent the second body 23 from blocking the plurality of liquid injection holes 21, and when the needle of the syringe 202 is inserted into the liquid injection hole 21, the liquid reagent can be smoothly injected into the sample area of ​​the placement groove 11. By setting the second body 23 in annular shape, the cover body 20 and the adapter body 10 can be accurately aligned, and the risk of displacement of the cover body 20 can also be reduced.

[0071] In some embodiments of the present invention, the thickness dimension of the second body 23 can be 10 mm, and the depth dimension of the assembly hole 14 can be 15.5 mm. In this way, when the needle of a 1-inch flat-head syringe 202 is inserted into the liquid injection hole 21, it can be ensured that the needle mouth of the needle is 2 mm away from the bottom of the placement groove 11 of the second body 10, preventing the needle mouth from being blocked and the liquid reagent from being unable to be ejected.

[0072] In some embodiments of the present invention, Figure 7 , Figure 8 and Fig.10 As shown, the adapter 100 may also include: a ring 31, the ring 31 defines a first through hole 311, the ring 31 is arranged in the placement groove 11, the ring 31 is suitable for overlapping with the sample carrier 200 to form a sample groove in the placement groove 11, the sample groove is suitable for placing the sample, and the drainage hole 12 and the multiple liquid injection holes 21 are all connected to the sample groove.

[0073] The collar 31 may be made of polytetrafluoroethylene. When the sample carrier 200 is placed in the placement groove 11, the collar 31 is assembled in the placement groove 11, the collar 31 is located on the side of the sample carrier 200 facing the cover 20, the collar 31 and the sample carrier 200 are overlapped, and a sample groove may be formed in the placement groove 11, one end of the sample groove is open, and the sample groove is defined by the collar 31 and the sample carrier 200, the sample area of ​​the sample carrier 200 corresponds to the first through hole 311, and the sample is located in the sample area so that the sample is placed in the sample groove.

[0074] Specifically, at least a portion of the sample carrier 200 carrying the sample is placed in the placement groove 11, so that the sample is located in the placement groove 11, and the collar 31 is assembled in the placement groove 11, and the collar 31 and the sample carrier 200 define a sample groove, and the sample is located in the sample groove. The cover body 20 is covered on the open end of the placement groove 11, and after the liquid reagents required for sample preparation are loaded into the multiple syringes 202 of the automatic sample preparation machine, the needles of the multiple syringes 202 are respectively inserted into the multiple liquid injection holes 21, and the automatic sample preparation machine can respectively inject the corresponding liquid reagents into the sample groove through the multiple liquid injection holes 21. By providing the collar 31, the collar 31 and the sample carrier 200 can define a sample groove, and the volume of the sample groove is smaller than the volume of the placement groove 11, which is conducive to full contact between the liquid reagent and the sample.

[0075] In some embodiments of the present invention, Figure 7 and Fig.10 As shown, the open end of the sample slot can be opposite to the liquid discharge hole 12 and the plurality of liquid injection holes 21 , and such an arrangement facilitates the injection of corresponding liquid reagents into the sample slot.

[0076] In some embodiments of the present invention, Figure 7and Fig.10 As shown, when the cover 20 is covered on the open end of the placement groove 11, the cover 20 abuts against the collar 31. When the sample carrier 200 is placed in the placement groove 11, the collar 31 is assembled in the placement groove 11, and the collar 31 overlaps with the sample carrier 200. When the cover 20 is covered on the open end of the placement groove 11, the cover 20 abuts against the collar 31, and the cover 20 presses against the collar 31, which is beneficial to improve the sealing of the sample groove and reduce the risk of liquid reagent in the sample groove flowing into the placement groove 11.

[0077] In some embodiments of the present invention, Figure 7 and Fig.10 As shown, the adapter 100 may further include: a sealing ring 32, which is suitable for being assembled between the end of the collar 31 away from the cover 20 and the sample carrier 200, and the sealing ring 32 is arranged around the first through hole 311. The sealing ring 32 may be made of materials such as rubber and polyurethane. When the sample carrier 200 is placed in the placement groove 11, the collar 31 is assembled in the placement groove 11, and the sealing ring 32 is located between the end of the collar 31 away from the cover 20 and the sample carrier 200. When the cover 20 is covered on the open end of the placement groove 11, the cover 20 abuts against the collar 31, and the cover 20 presses the collar 31 and the sealing ring 32, so that the sealing ring 32 is assembled between the end of the collar 31 away from the cover 20 and the sample carrier 200. The sealing ring 32 can seal the gap between the collar 31 and the sample carrier 200, further reducing the risk of the liquid reagent in the sample groove flowing into the placement groove 11, and improving the sealing of the sample groove.

[0078] In some embodiments of the present invention, Figure 8 As shown, a limiting ring 33 is formed at the end of the collar 31 away from the cover body 20, the limiting ring 33 is arranged around the first through hole 311, and the sealing ring 32 is assembled on the limiting ring 33. The limiting ring 33 can be integrally formed with the collar 31, the sealing ring 32 can be sleeved on the outside of the limiting ring 33, or the sealing ring 32 can be embedded in the end of the limiting ring 33 facing the sample carrier 200. By arranging the limiting ring 33 on the collar 31, the sealing ring 32 can be assembled on the collar 31, so that the collar 31 and the sealing ring 32 are fixed, and the risk of the sealing ring 32 moving between the collar 31 and the sample carrier 200 is reduced, so that the sealing ring 32 can better seal the gap between the collar 31 and the sample carrier 200.

[0079] In some embodiments of the present invention, Figure 7 and Fig.10 As shown, the adapter 100 may further include: a waste liquid discharge pipe 34, the cover body 20 is formed with a liquid discharge hole 12, the waste liquid discharge pipe 34 is penetrated through the liquid discharge hole 12 and one end extends into the sample tank, and the waste liquid discharge pipe 34 abuts against the bottom wall of the sample tank.

[0080] The waste liquid discharge pipe 34 can be made of steel, the sample carrier 200 is configured as the bottom wall of the sample tank, the waste liquid discharge pipe 34 is arranged through the drainage hole 12, one end of the waste liquid discharge pipe 34 extends into the sample tank and abuts against the sample carrier 200, and the other end of the waste liquid discharge pipe 34 is located outside the adapter 100, and the waste liquid discharge pipe 34 can be connected to the second peristaltic pump 204. When the liquid in the sample tank needs to be discharged, the second peristaltic pump 204 works, and the liquid reagent in the placement tank 11 can be drawn out through the waste liquid discharge pipe 34, so that the liquid reagent in the sample tank is discharged from the sample tank, which is conducive to the discharge of waste liquid in the sample tank. When it is not necessary to discharge the liquid in the placement tank 11, the second peristaltic pump 204 does not work.

[0081] In some embodiments of the present invention, a suction port is provided at one end of the waste liquid discharge tube 34 extending into the sample slot, and the suction port is arranged at an inclined port. When the waste liquid discharge tube 34 abuts against the sample carrier 200, the suction port can be prevented from being blocked, so that the waste liquid discharge tube 34 can normally suck the liquid reagent in the sample slot, thereby improving the working performance of the waste liquid discharge tube 34.

[0082] In some embodiments of the present invention, Figure 7 and Fig.10 As shown, the cover body 20 has a cover body end wall 35, and the cover body end wall 35 is formed with a liquid injection hole 21 and a drainage hole 12. Along the depth direction of the placement groove 11, the cover body end wall 35 is opposite to the bottom wall of the placement groove 11. By arranging the liquid injection hole 21 on the cover body end wall 35, the liquid injection hole 21 can be opposite to the sample groove, which is convenient for the liquid reagent to flow into the sample groove. By arranging the drainage hole 12 on the cover body end wall 35, the drainage hole 12 can be opposite to the sample groove, which is convenient for the waste liquid discharge pipe 34 to extend into the sample groove through the drainage hole 12.

[0083] An assembly groove 351 is formed on the outer surface of the cover end wall 35, the drainage hole 12 is located at the assembly groove 351, and at least part of the waste liquid discharge pipe 34 is assembled in the assembly groove 351. The waste liquid discharge pipe 34 can be an L-shaped structure, the drainage hole 12 is located at the bottom wall of the assembly groove 351, and the waste liquid discharge pipe 34 is penetrated through the drainage hole 12. After the waste liquid discharge pipe 34 is assembled in place, at least part of the structure of the waste liquid discharge pipe 34 is assembled in the assembly groove 351. The side wall of the assembly groove 351 can limit the waste liquid discharge pipe 34, which can reduce the risk of the waste liquid discharge pipe 34 rotating, thereby improving the reliability of the connection between the waste liquid discharge pipe 34 and the second peristaltic pump 204.

[0084] In some embodiments of the present invention, Figure 7 and Fig.10As shown, the adapter 100 may further include: an elastic pressing piece 36, the elastic pressing piece 36 is located on the side of the waste liquid discharge pipe 34 away from the cover end wall 35, and one end of the elastic pressing piece 36 is fixed to the cover end wall 35, and the elastic pressing piece 36 presses against the waste liquid discharge pipe 34. The elastic pressing piece 36 is located outside the cover 20, one end of the elastic pressing piece 36 can be fixed to the cover end wall 35 by bolts, or one end of the elastic pressing piece 36 can be clamped to the cover end wall 35, and the elastic pressing piece 36 can be made of a metal spring sheet, for example: the elastic pressing piece 36 is made of a metal material such as stainless steel, aluminum, etc. The waste liquid discharge pipe 34 is located between the elastic pressing piece 36 and the cover end wall 35, the elastic pressing piece 36 is elastic, and the elastic pressing piece 36 can press against the waste liquid discharge pipe 34, so that the waste liquid discharge pipe 34 is firmly assembled on the cover 20. The force applied by the elastic pressing sheet 36 to the waste liquid discharge pipe 34 can always ensure that the waste liquid discharge pipe 34 is against the sample carrier 200, thereby achieving the effect of draining out the waste liquid in the sample tank.

[0085] Furthermore, if Figure 7 and Fig.10 As shown, an elastic mounting groove 38 is also formed on the outer surface of the cover end wall 35, and the elastic mounting groove 38 is connected to the assembly groove 351. At least a portion of the elastic pressing piece 36 can be assembled in the elastic mounting groove 38. The inner wall of the elastic mounting groove 38 can limit the elastic pressing piece 36 to reduce the risk of rotation of the elastic pressing piece 36.

[0086] In some embodiments of the present invention, Figure 7 and Fig.10 As shown, the cover body 20 also has an annular cover body side wall 37, which is arranged around the circumferential edge of the cover body end wall 35, and the outer surface of the cover body side wall 37 is formed with a first threaded portion 371, and the inner wall of the placement groove 11 is formed with a second threaded portion 112, and the second threaded portion 112 is adjacent to the open end of the placement groove 11, and the first threaded portion 371 and the second threaded portion 112 are assembled together to fix the cover body 20 and the adapter body 10.

[0087] When the cover 20 and the adapter body 10 are assembled, the first threaded portion 371 and the second threaded portion 112 can be matched and connected by rotating the cover 20, so that the cover 20 and the adapter body 10 are firmly connected, and the cover 20 and the adapter body 10 are prevented from being separated. In addition, when the cover 20 is rotated to assemble the cover 20 and the adapter body 10, the cover 20 can squeeze the collar 31, so that the sealing ring 32 and the sample carrier 200 can be reliably stopped, so that the gap between the cover 20 and the adapter body 10 is sealed, and the risk of leakage of the liquid reagent in the sample tank from the gap between the cover 20 and the adapter body 10 is reduced.

[0088] In some embodiments of the present invention, Fig.10As shown, the side wall of the placement groove 11 is formed with a second through hole 113 that is connected to the placement groove 11, and the sample carrier 200 is assembled in the placement groove 11 through the second through hole 113. In this embodiment, the sample carrier 200 is configured as a tissue section slide, and the adapter 100 is adapted to the tissue section slide. When the adapter 100 is assembled with the tissue section slide, the tissue section slide is inserted into the placement groove 11 through the second through hole 113, so that at least part of the tissue section slide is assembled in the placement groove 11, and the sample area on the tissue section slide is located in the placement groove 11. The second through hole 113 is formed on the side wall of the placement groove 11, so that the tissue section slide is easily assembled in the placement groove 11, so that the adapter 100 is adapted to the tissue section slide.

[0089] In some embodiments of the present invention, Fig.10 As shown, there are two second through holes 113, and the two second through holes 113 are arranged opposite to each other. The two second through holes 113 are arranged at intervals. When the adapter 100 is assembled with the tissue section slide, the tissue section slide is inserted into the placement groove 11 through one second through hole 113, and the tissue section slide passes through the two second through holes 113 at the same time. The inner walls of the two second through holes 113 can limit the tissue section slide, reducing the risk of the tissue section slide rotating.

[0090] In some embodiments of the present invention, a plurality of liquid injection holes 21 are arranged along the circumference of the cover body 20. Figure 2 As shown, in this embodiment, a plurality of liquid injection holes 21 are arranged along the circumference of the first body 22. Figure 7 and Fig.10 As shown, in the two embodiments, a plurality of liquid injection holes 21 are arranged along the circumference of the cover end wall 35. The plurality of liquid injection holes 21 can be arranged in sequence along the circumference of the cover 20, so that the needle of the syringe 202 can be smoothly inserted into the liquid injection hole 21.

[0091] In some embodiments of the present invention, each liquid injection hole 21 is spaced apart from the central axis of the cover body 20. Figure 2 As shown, in this embodiment, each liquid injection hole 21 is spaced apart from the central axis of the first body 22. Figure 7 and Fig.10 As shown, in the two embodiments, each liquid injection hole 21 is spaced apart from the central axis of the cover end wall 35. In the radial direction of the cover 20, each liquid injection hole 21 is spaced apart from the central axis of the cover 20. Such a setting can make two adjacent liquid injection holes 21 spaced apart, making it easier for the needle of the syringe 202 to be smoothly inserted into the liquid injection hole 21, and when the needle of the syringe 202 is inserted into the liquid injection hole 21, it can avoid collision between two adjacent syringes 202.

[0092] In some embodiments of the present invention, the distance between the central axis of the liquid injection hole 21 and the central axis of the cover body 20 is D, which satisfies the relationship: 7mm≤D≤8mm. The distance between the central axis of the liquid injection hole 21 and the central axis of the cover body 20 can be 7mm, 7.5mm, 8mm, etc. Figure 2 As shown, in this embodiment, the distance between the central axis of the liquid injection hole 21 and the central axis of the first body 22 is D. Figure 7 and Fig.10 As shown, in the two embodiments, the spacing distance between the central axis of the liquid injection hole 21 and the central axis of the cover end wall 35 is D. Among them, the central axis of the liquid injection hole 21 and the central axis of the cover 20 both extend along the thickness direction of the cover 20, and the spacing distance between the central axis of the liquid injection hole 21 and the central axis of the cover 20 can be reasonably selected and set according to actual conditions. By setting the spacing distance between the central axis of the liquid injection hole 21 and the central axis of the cover 20 to D, it is more conducive to the smooth insertion of the needle of the syringe 202 into the liquid injection hole 21.

[0093] like Figure 7 and Fig.10 As shown, the bottom wall of the placement groove 11 is formed with an observation hole 15, through which the position of the sample on the sample carrier 200 can be observed.

[0094] According to the following Figure 2 , Figure 7 and Fig.10 Three specific embodiments according to the present application are described.

[0095] like Figure 2 As shown, according to the first embodiment of the utility model, in this embodiment, the adapter 100 is adapted to fit the glass slide. The adapter 100 includes an adapter body 10, a cover body 20 and a connecting joint 13. The adapter body 10 defines a placement groove 11, and the adapter body 10 is formed with an assembly hole 14 and a drainage hole 12, and the connecting joint 13 is installed in the drainage hole 12. The cover body 20 includes a first body 22 and a second body 23, and the first body 22 is formed with a liquid injection hole 21, and the liquid injection hole 21 is opposite to the open end of the placement groove 11. The second body 23 is assembled in the assembly hole 14. When preparing the sample, the glass slide is placed in the placement groove 11.

[0096] like Figure 7As shown, according to the second embodiment of the utility model, in this embodiment, the adapter 100 is adapted to the confocal dish 2001, the confocal dish 2001 is a sample carrier 200, the confocal dish 2001 includes a dish bottom wall 2011 and an annular dish side wall 2012, the dish side wall 2012 is arranged along the circumferential edge of the dish bottom wall 2011, the dish bottom wall 2011 and the annular dish side wall 2012 define a groove structure with one end open, and the dish bottom wall 2011 is constructed as the bottom wall of the sample groove. The adapter 100 includes an adapter body 10, a cover body 20, a collar 31, a sealing ring 32, a waste liquid discharge pipe 34, and an elastic pressing sheet 36. The confocal dish 2001 is placed in the placement groove 11, the collar 31 is arranged in the placement groove 11 and is located in the groove structure of the confocal dish 2001, and the collar 31 and the dish bottom wall 2011 define the sample groove. The cover end wall 35 is formed with a liquid injection hole 21 and a liquid discharge hole 12. A waste liquid discharge pipe 34 is arranged through the liquid discharge hole 12 and extends into the sample tank. One end of the elastic pressing piece 36 is fixed to the cover end wall 35. The elastic pressing piece 36 presses against the waste liquid discharge pipe 34. The cover 20 and the adapter body 10 are threadedly connected.

[0097] The cross section of the adapter body 10 can be circular, and the adapter body 10 can be a threaded cylindrical barrel structure with a diameter of 40mm and a height of 14mm. The collar 31 is set to a circular ring structure with a height of 6.9mm, an outer diameter of 35mm, and an inner diameter of 14mm. The limit ring 33 is set to a height of 1.7mm, an inner diameter of 14mm, and an outer concave arc structure, which can clamp the sealing ring 32 and prevent it from falling. Through the combination of the collar 31 and the sealing ring 32, a sample groove can be formed in the imaging area of ​​the confocal dish 2001, and the sample groove can be set to a diameter of 14mm and a height of 8.6mm.

[0098] The cross section of the cover body 20 can be circular, and the cover body 20 can be set to a diameter of 48mm and a height of 15mm. The cover body 20 and the adapter body 10 are threadedly connected. By rotating the cover body 20, the cover body 20 and the adapter body 10 can be disassembled and assembled to squeeze the collar 31, thereby sealing the sample slot and preventing the liquid in the sample slot from overflowing. There are multiple liquid injection holes 21 in the center of the cover body end wall 35. The diameter of the multiple liquid injection holes 21 can be set to 0.8mm. The multiple liquid injection holes 21 can be arranged on the same circle to achieve that when the syringe 202 is inserted into the corresponding liquid injection hole 21, the needles of all syringes 202 can be close to the inner hole edge of the collar 31. The bottom wall of the placement groove 11 is formed with an observation hole 15.

[0099] like Fig.10As shown, according to the third embodiment of the utility model, in this embodiment, the adapter 100 is adapted to fit the tissue section slide, and the tissue section slide is a sheet structure. The adapter 100 of the third embodiment is different from the adapter 100 of the second embodiment in that: in the third embodiment, the side wall of the placement groove 11 is formed with a second through hole 113 connected to the placement groove 11.

[0100] The automatic sample making machine according to the embodiment of the utility model comprises: an adapter 100, a first peristaltic pump 201, a plurality of syringes 202, a second peristaltic pump 204, a controller 206 and a liquid storage bottle 205. The adapter 100 is the adapter 100 of the above embodiment. An electromagnetic valve 203 is connected between each syringe 202 and the first peristaltic pump 201, and the syringe outlets of the plurality of syringes 202 are suitable for being connected to the placement slot 11. The second peristaltic pump 204 is connected between the liquid storage bottle 205 and the drainage hole 12. The controller 206 is configured to control the operation of the first peristaltic pump 201 and the second peristaltic pump 204, and is also configured to control the electromagnetic valve 203 to be opened or closed.

[0101] The first peristaltic pump 201 and the second peristaltic pump 204 have the same structure. The number of the plurality of syringes 202 and the number of the plurality of liquid injection holes 21 can be the same, and one liquid injection hole 21 can be equipped with one syringe 202. The liquid storage bottle 205 is used to store waste liquid. The number of the electromagnetic valves 203 and the number of the syringes 202 are the same, and each syringe 202 and the first peristaltic pump 201 are connected with an electromagnetic valve 203, and the needle of the syringe 202 is formed with a syringe outlet, and the needle of the syringe 202 is inserted into the liquid injection hole 21 so that the syringe outlet is connected to the placement slot 11, and the second peristaltic pump 204 is connected between the liquid storage bottle 205 and the liquid discharge hole 12. The controller 206 is connected to the first peristaltic pump 201 and the second peristaltic pump 204 in communication, and the controller 206 can control the first peristaltic pump 201 and the second peristaltic pump 204 to work, and the controller 206 can also control the electromagnetic valve 203 to open or close.

[0102] Specifically, the host computer control software is installed in the controller 206 (such as a computer), the controller 206 can be connected to the single-chip microcomputer via a USB data cable, a relay is connected between each solenoid valve 203 and the single-chip microcomputer, and the single-chip microcomputer can be connected to the relay via a wire.

[0103] The first peristaltic pump 201 is used to control the absorption and push-out of the liquid reagent in the syringe 202. The first peristaltic pump 201 can be connected to the controller 206 through the communication module. One end of the first peristaltic pump 201 can be connected to the air, and the other end of the first peristaltic pump 201 can be connected to the air inlet of the corresponding electromagnetic valve 203 through multiple first silicone tubes. The air outlet of each electromagnetic valve 203 is connected to a second silicone tube, and the end of each second silicone tube away from the corresponding electromagnetic valve 203 is connected to the syringe 202 connector. The syringe 202 connector is inserted into the corresponding syringe 202 to achieve the connection between the air path and the syringe 202. During operation, the required volume of liquid reagent is sucked into the syringe 202, and then the needle of the syringe 202 is inserted into the corresponding liquid injection hole 21. The second peristaltic pump 204 can be connected to the controller 206 through the communication module. The waste liquid inlet of the second peristaltic pump 204 can be connected to the connecting joint 13 or the waste liquid discharge pipe 34 through the first rubber hose, and the waste liquid outlet of the second peristaltic pump 204 can be inserted into the waste liquid bottle through the second rubber hose.

[0104] The first peristaltic pump 201 is used to control the suction and push of liquid reagents in multiple syringes 202. Before sample preparation, the needle of the syringe 202 is inserted into the corresponding liquid reagent storage device, and then the controller 206 controls the first peristaltic pump 201 to work, thereby controlling the syringe 202 to absorb the liquid reagent in the liquid reagent storage device. After the syringe 202 absorbs the liquid reagent, the needle of the syringe 202 is inserted into the corresponding liquid injection hole 21. The controller 206 controls the corresponding electromagnetic gas valve 203 to open by controlling the relay, and the other electromagnetic gas valves 203 are still in a closed state, and the controller 206 also controls the first peristaltic pump 201 to work to fill the corresponding syringe 202 with gas to push the liquid reagent in the corresponding syringe 202 into the placement tank 11. By analogy, by controlling the electromagnetic gas valves 203 of different paths to open, different liquid reagents can be injected into the placement tank 11 in sequence to complete sample preparation. The controller 206 can also control the second peristaltic pump 204 to work. When the second peristaltic pump 204 is working, the waste liquid in the placement tank 11 can be pumped into the liquid storage bottle 205. When the second peristaltic pump 204 is not working, the liquid in the placement tank 11 cannot flow out of the placement tank 11.

[0105] According to the automatic sample preparation machine of the utility model, by setting an adapter 100, a first peristaltic pump 201, a plurality of syringes 202, a second peristaltic pump 204, a controller 206 and a liquid storage bottle 205, automatic sample preparation can be achieved without the presence of experimenters, which greatly saves labor and reduces the impact of repetitive labor on the physical and mental health of experimenters. In addition, by setting a drainage hole 12, the liquid reagent in the placement groove 11 can be discharged from the placement groove 11, which is conducive to the discharge of waste liquid in the placement groove 11.

[0106] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0107] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. An adapter for an automatic sample making machine, characterized in that: include: An adapter body, wherein the adapter body defines a placement slot with one end open, and the placement slot is used to place the sample carrier; A cover body is provided on the open end of the placement groove, and the cover body is formed with a plurality of liquid injection holes, and the plurality of liquid injection holes are all connected with the placement groove, and at least one of the adapter body and the cover body is formed with a drainage hole connected with the placement groove.

2. The adapter of the automatic sample making machine according to claim 1, characterized in that: The cover body includes a first main body, which is covered at the open end of the placement groove and is formed with a plurality of the liquid injection holes.

3. The adapter of the automatic sample making machine according to claim 2, characterized in that: The cover body also includes a second main body, which is overlapped with the first main body and is located on the side of the first main body facing the adapter main body. The adapter main body is formed with an assembly hole opposite to and connected to the open end of the placement groove. The assembly hole is located on the side of the open end of the placement groove facing the cover body. When the cover body is covered on the open end of the placement groove, the second main body is assembled in the assembly hole and abuts against the inner peripheral wall of the assembly hole.

4. The adapter of the automatic sample making machine according to claim 3, characterized in that: The second body is annular, and along the stacking direction of the second body and the first body, the orthographic projections of the plurality of liquid injection holes are all located within the orthographic projection range of the second body.

5. The adapter of the automatic sample making machine according to claim 1, characterized in that: The adapter body is formed with the drainage hole, and the drainage hole extends to the bottom wall surface of the placement groove.

6. The adapter of the automatic sample making machine according to claim 1, characterized in that: Also includes: A collar, wherein the collar defines a first through hole, the collar is disposed in the placement groove, the collar is suitable for overlapping with the sample carrier to form a sample groove in the placement groove, the sample groove is suitable for placing a sample, and the drainage hole and the plurality of liquid injection holes are all connected to the sample groove.

7. The adapter of the automatic sample making machine according to claim 6, characterized in that: The open end of the sample slot is opposite to the liquid discharge hole and the plurality of liquid injection holes.

8. The adapter of the automatic sample making machine according to claim 6, characterized in that: Also includes: A sealing ring is adapted to be assembled between the end of the collar facing away from the cover body and the sample carrier, and the sealing ring is arranged around the first through hole.

9. The adapter of the automatic sample making machine according to claim 6, characterized in that: Also includes: The waste liquid discharge pipe is formed with the liquid discharge hole in the cover body, the waste liquid discharge pipe is passed through the liquid discharge hole and one end of the waste liquid discharge pipe extends into the sample tank, and the waste liquid discharge pipe abuts against the bottom wall of the sample tank.

10. The adapter of the automatic sample making machine according to claim 9, characterized in that: The cover body has a cover body end wall, the cover body end wall is formed with the liquid injection hole and the liquid discharge hole, and along the depth direction of the placement groove, the cover body end wall is opposite to the bottom wall of the placement groove; An assembly groove is formed on the outer surface of the end wall of the cover body, the liquid discharge hole is located at the assembly groove, and at least a part of the waste liquid discharge pipe is assembled in the assembly groove.

11. The adapter of the automatic sample making machine according to claim 10, characterized in that: Also includes: An elastic pressing sheet is located on the side of the waste liquid discharge pipe away from the end wall of the cover body, and one end of the elastic pressing sheet is fixed to the end wall of the cover body, and the elastic pressing sheet presses against the waste liquid discharge pipe.

12. The adapter of the automatic sample making machine according to claim 10, characterized in that: The cover body also has an annular cover body side wall, which is arranged around the circumferential edge of the cover body end wall, and the outer surface of the cover body side wall is formed with a first threaded portion, and the inner wall of the placement groove is formed with a second threaded portion, and the second threaded portion is adjacent to the open end of the placement groove, and the first threaded portion and the second threaded portion are assembled together to fix the cover body and the adapter body.

13. The adapter of the automatic sample making machine according to any one of claims 6 to 12, characterized in that: A second through hole communicating with the placement groove is formed on the side wall of the placement groove, and the sample carrier is assembled in the placement groove through the second through hole.

14. An automatic sample making machine, characterized in that: include: An adapter, wherein the adapter is an adapter according to any one of claims 1 to 13; A first peristaltic pump and a plurality of syringes, each of the syringes and the first peristaltic pump is connected with an electromagnetic valve, and the syringe outlets of the plurality of syringes are adapted to communicate with the placement slot; a second peristaltic pump and a liquid storage bottle, wherein the second peristaltic pump is connected between the liquid storage bottle and the liquid discharge hole; A controller is configured to control the first peristaltic pump and the second peristaltic pump to operate, and is also configured to control the electromagnetic valve to open or close.

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