A centrifugal extraction device for stem cell exosomes

Abstract

The utility model discloses a centrifugal extraction device for stem cell exosomes, comprising a bottom frame, a top frame and a bracket, and also comprising a round tube, one end of the round tube is movably connected with a top cover, the inner diameter of the other end is movably sleeved with a sleeve, and the bottom end of the sleeve is fixedly connected with a discharge hopper; a motor is installed on the top frame for driving the round tube to rotate; a support tube is vertically installed on the upper side of the bottom frame for supporting the discharge hopper; a vertical drive assembly is installed on the top frame for driving the round tube to move in a vertical direction; a liquid outlet is provided on the top side wall of the round tube. The utility model automatically separates the supernatant and the precipitate after the stem cell culture fluid is centrifuged in the device, and both centrifugation and liquid separation can be performed in the device, which has a simple structure, is convenient and practical, and is easy to promote and apply.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, and in particular to a centrifugal extraction device for stem cell exosomes. Background Art

[0002] Stem cell exosomes are a type of extracellular vesicles with a complete membrane structure secreted by stem cells, containing a variety of lipids, proteins, RNA and other bioactive factors, with a diameter of about 30 to 150 nm. Stem cell exosomes have similar biological properties to stem cells, but compared with stem cells, exosomes have lower immunogenicity, are safer, more stable and efficient, and have high development and application potential in the fields of modern biology and medicine. At present, stem cell exosomes are mainly extracted from the culture fluid harvested from cultured stem cells. When extracting stem cell exosomes by centrifugation, the supernatant must be taken after centrifugation. In the prior art, centrifugation and separation usually require two devices to operate the container separately, which is complicated and inconvenient to operate. The utility model provides a centrifugal extraction device for stem cell exosomes. Utility Model Content

[0003] In order to solve the technical problems existing in the background technology, the utility model proposes a centrifugal extraction device for stem cell exosomes.

[0004] The utility model provides a centrifugal extraction device for stem cell exosomes, comprising a bottom frame, a top frame and a bracket, wherein the top frame is located above the bottom frame, and a plurality of brackets are fixedly connected between the top frame and the bottom frame, and further comprising a round tube, wherein one end of the round tube is movably connected to a top cover, and the inner diameter of the other end thereof is movably sleeved with a sleeve, and the bottom end of the sleeve is fixedly connected to a discharge hopper;

[0005] A motor is installed on the top frame, a turntable is connected to the motor, and the top cover is movably connected to the turntable;

[0006] A support pipe is vertically installed on the upper side of the base frame to support the discharge hopper;

[0007] The discharge hopper is conical, the lower outlet nozzle of the discharge hopper is located in the inner circle of the upper end of the support tube, the upper end of the support tube is supported on the side of the discharge hopper, the outer diameter of the support tube is smaller than the inner diameter of the circular tube, and a control valve is installed on the discharge hopper;

[0008] A vertical driving assembly is installed on the top frame to drive the round tube to move in the vertical direction;

[0009] A liquid outlet hole is provided on the top side wall of the circular tube.

[0010] Furthermore, a rubber ring is fixedly connected to the upper outer wall of the sleeve, and the rubber ring is tightly fitted to the inner wall of the circular tube.

[0011] Furthermore, a plurality of groups of first rollers are installed on the upper end surface of the support tube, and the first rollers are centrally distributed on the end surface of the support tube.

[0012] Preferably, a plurality of latching keys are fixedly connected to the bottom of the turntable, a plurality of latching slots are formed on the top of the top cover, and the latching keys correspond to the latching slots one by one and are movably latched.

[0013] Preferably, the outer side wall of the circular tube has a limiting ring extending radially outward, and the vertical drive assembly is provided with a clamping block, the clamping block is provided with a groove, and the limiting ring is clamped in the groove of the clamping block.

[0014] Preferably, the vertical drive assembly is provided with two groups, which are located on both sides of the circular tube, and the two groups of clamping blocks are respectively clamped on both sides of the limiting ring.

[0015] Furthermore, a plurality of second rollers are installed in the groove of the block.

[0016] Preferably, the liquid outlet is movably connected to a guide pipe.

[0017] In the utility model, a centrifugal extraction device for stem cell exosomes is proposed, in which a vertical driving component can drive the circular tube to move in the vertical direction. When the circular tube moves upward, after the top cover of the circular tube is docked with the turntable, the motor can drive the circular tube to rotate with its axis as the rotation axis through the top cover, which can play a centrifugal role; when the centrifugation is completed, after standing for a period of time, it is allowed to precipitate into the lower hopper at the bottom of the sleeve; at this time, the vertical driving component drives the circular tube to move downward, and the sleeve enters the circular tube. Since the outer diameter of the support tube is less than the inner diameter of the circular tube, the support tube will also enter the tube of the circular tube, and the supernatant of the stem cell culture solution after centrifugation will be discharged from the liquid outlet hole on the top side wall of the circular tube, and the sediment in the lower hopper can be discharged from the lower outlet nozzle of the lower hopper. The present application can automatically separate the supernatant and sediment after centrifugation of the stem cell culture solution in the device, and has a simple structure, is convenient and practical, and is easy to promote and apply.

[0018] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2 It is a schematic diagram of the structure of the utility model when discharging liquid.

[0021] Figure 3 It is a schematic diagram of the structure of the utility model when discharging liquid.

[0022] Figure 4 It is a partial structural schematic diagram of the utility model.

[0023] Figure 5 It is a structural schematic diagram of the clamping block in the utility model.

[0024] Figure 6 It is a top view of the support tube in the utility model.

[0025] Description of the numbers in the figure:

[0026] 1. bottom frame; 2. bracket; 3. top frame; 4. motor; 5. turntable; 501. key;

[0027] 6. Round tube; 7. Top cover; 701. Slot; 8. Limiting ring; 9. Casing; 901. Rubber ring; 10. Discharging hopper; 11. Control valve;

[0028] 12. Support tube; 1201. First roller; 13. Block; 1301. Second roller; 14. Vertical drive assembly; 15. Export tube. DETAILED DESCRIPTION

[0029] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar symbols throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0030] like Figure 1-Figure 6 A centrifugal extraction device for stem cell exosomes shown in the figure comprises a bottom frame 1, a top frame 3 and a bracket 2, wherein the top frame 3 is located above the bottom frame 1, and a plurality of brackets 2 are fixedly connected between the top frame 3 and the bottom frame 1, and further comprises a round tube 6, wherein one end of the round tube 6 is movably connected to a top cover 7, and the inner diameter of the other end thereof is movably sleeved with a sleeve 9, and the bottom end of the sleeve 9 is fixedly connected to a discharge hopper 10;

[0031] The top cover 7 is connected to the upper end of the circular tube 6 by threads, the outer tube wall of the sleeve 9 can move in the inner wall of the circular tube 6, and the discharge hopper 10 at the bottom of the sleeve 9 is set in a funnel shape.

[0032] The top frame 3 is provided with a motor 4, the motor 4 is connected with a turntable 5, and the top cover 7 is movably connected with the turntable 5;

[0033] The stem cell culture medium is introduced into the cylinder.

[0034] When the turntable 5 is docked with the top cover 7, the motor 4 can drive the round tube 6 to rotate with its axis as the rotation axis through the top cover 7, which can play a centrifugal role.

[0035] A support pipe 12 is vertically mounted on the upper side of the base frame 1 to support the discharge hopper 10;

[0036] The discharge hopper 10 is conical, and the lower outlet nozzle of the discharge hopper 10 is located in the inner circle of the upper end of the support tube 12. The upper end of the support tube 12 is supported on the side of the discharge hopper 10. The outer diameter of the support tube 12 is smaller than the inner diameter of the circular tube 6. The control valve 11 is installed on the discharge hopper 10.

[0037] The discharge hopper 10 is conical in shape, and the lower outlet nozzle of the discharge hopper 10 is inserted into the upper tube opening of the support tube 12 . The upper end tube wall of the support tube 12 can support the outer conical side wall of the discharge hopper 10 .

[0038] A vertical driving assembly 14 is installed on the top frame 3 to drive the round tube 6 to move in the vertical direction;

[0039] The top side wall of the circular tube 6 is provided with a liquid outlet.

[0040] The vertical drive assembly 14 can drive the round tube 6 to move in the vertical direction. When the round tube 6 moves upward, after the top cover 7 of the round tube 6 is docked with the turntable 5, the motor 4 can drive the round tube 6 to rotate with its axis as the rotation axis through the top cover 7, which can play a centrifugal role.

[0041] After the centrifugation is completed, the mixture is allowed to stand for a period of time to settle in the lower hopper at the bottom of the casing 9;

[0042] At this time, the vertical driving assembly 14 drives the circular tube 6 to move downward, and the sleeve 9 enters into the circular tube 6. Since the outer diameter of the support tube 12 is smaller than the inner diameter of the circular tube 6, the support tube 12 will also enter into the circular tube 6, and the supernatant of the stem cell culture fluid after centrifugation will be discharged from the liquid outlet hole on the top side wall of the circular tube 6, and the sediment in the lower hopper can be discharged from the lower outlet nozzle of the lower hopper.

[0043] Furthermore, a rubber ring 901 is fixedly connected to the upper outer wall of the sleeve 9 , and the rubber ring 901 is tightly fitted to the inner wall of the circular tube 6 .

[0044] The sealing between the outer wall of the sleeve 9 and the inner wall of the circular tube 6 can be enhanced.

[0045] Furthermore, a plurality of groups of first rollers 1201 are installed on the upper end surface of the support tube 12 , and the first rollers 1201 are centrally distributed on the end surface of the support tube 12 .

[0046] The friction between the upper side of the support tube 12 and the outer wall of the lower hopper can be reduced.

[0047] Preferably, a plurality of latching keys 501 are fixedly connected to the bottom of the turntable 5 , and a plurality of latching slots 701 are opened on the top of the top cover 7 . The latching keys 501 correspond to the latching slots 701 one by one and are movably latched.

[0048] When the turntable 5 is docked with the top cover 7 , the latching key 501 on the turntable 5 is latched with the latching slot 701 on the top cover 7 , which can ensure that the motor 4 drives the round tube 6 to rotate through the turntable 5 .

[0049] Preferably, the outer wall of the circular tube 6 has a limiting ring 8 extending radially outward, and the vertical drive assembly 14 is provided with a clamping block 13 , the clamping block 13 has a groove, and the limiting ring 8 is clamped in the groove of the clamping block 13 .

[0050] The vertical drive assembly 14 will not affect the rotation of the round tube 6 driven by the motor 4 while driving the round tube 6 to move in the vertical direction.

[0051] Preferably, the vertical drive assembly 14 is provided with two groups, which are located on both sides of the circular tube 6. The two groups of the clamping blocks 13 are respectively clamped on both sides of the limiting ring 8. The clamping blocks 13 on both sides can ensure the stability of the circular tube 6 during rotation.

[0052] Furthermore, a plurality of second rollers 1301 are installed in the groove of the clamping block 13 , and the second rollers 1301 can reduce the friction resistance between the limiting ring 8 and the clamping block 13 when the round tube 6 rotates.

[0053] Preferably, the liquid outlet is movably connected with a guide tube 15, which can guide the supernatant after centrifugation of the stem cell culture fluid into a storage container.

[0054] It should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0055] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present utility model, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0056] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0057] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0058] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A centrifugal extraction device for stem cell exosomes, comprising a bottom frame (1), a top frame (3) and a bracket (2), wherein the top frame (3) is located above the bottom frame (1), and a plurality of brackets (2) are fixedly connected between the top frame (3) and the bottom frame (1), characterized in that: It also comprises a round tube (6), one end of which is movably connected to a top cover (7), the inner diameter of the other end of which is movably sleeved with a sleeve (9), and the bottom end of which is fixedly connected to a discharge hopper (10); A motor (4) is installed on the top frame (3), a turntable (5) is connected to the motor (4), and the top cover (7) is movably connected to the turntable (5); A support pipe (12) is vertically mounted on the upper side of the base frame (1) for supporting the discharge hopper (10); The discharge hopper (10) is conical in shape, the lower outlet nozzle of the discharge hopper (10) is located in the inner circle of the upper end of the support tube (12), the upper end of the support tube (12) is supported on the side of the discharge hopper (10), the outer diameter of the support tube (12) is smaller than the inner diameter of the circular tube (6), and a control valve (11) is installed on the discharge hopper (10); A vertical driving assembly (14) is installed on the top frame (3) for driving the round tube (6) to move in the vertical direction; A liquid outlet hole is provided on the top side wall of the circular tube (6).

2. A centrifugal extraction device for stem cell exosomes according to claim 1, characterized in that: A rubber ring (901) is fixedly connected to the upper outer wall of the sleeve (9), and the rubber ring (901) is tightly fitted to the inner wall of the circular tube (6).

3. The centrifugal extraction device for stem cell exosomes according to claim 1, characterized in that: A plurality of groups of first rollers (1201) are installed on the upper end surface of the support tube (12), and the first rollers (1201) are centrally distributed on the end surface of the support tube (12).

4. The centrifugal extraction device for stem cell exosomes according to claim 1, characterized in that: The bottom of the turntable (5) is fixedly connected with a plurality of latching keys (501), and the top of the top cover (7) is provided with a plurality of latching slots (701). The latching keys (501) correspond to the latching slots (701) one by one and are movably latched.

5. The centrifugal extraction device for stem cell exosomes according to claim 1, characterized in that: The outer wall of the circular tube (6) has a limiting ring (8) extending radially outward, and the vertical drive assembly (14) is equipped with a clamping block (13), the clamping block (13) is provided with a groove, and the limiting ring (8) is clamped in the groove of the clamping block (13).

6. The centrifugal extraction device for stem cell exosomes according to claim 5, characterized in that: The vertical drive assembly (14) is provided with two groups, which are located on both sides of the circular tube (6), and the two groups of clamping blocks (13) are respectively clamped on both sides of the limiting ring (8).

7. The centrifugal extraction device for stem cell exosomes according to claim 5, characterized in that: A plurality of second rollers (1301) are installed in the groove of the clamping block (13).

8. The centrifugal extraction device for stem cell exosomes according to claim 1, characterized in that: The liquid outlet is movably connected to a delivery pipe (15).

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