A stirring device for nucleic acid extraction

Abstract

The utility model discloses a stirring device for nucleic acid extraction, which relates to the technical field of nucleic acid extraction and comprises a base, wherein a main ring is rotatably penetrated through the upper surface of the base, a main shaft is rotatably penetrated through the upper surface of the base, an inner ring surface of the main ring is fixedly sleeved on the upper end of the main shaft, a plurality of placement holes are opened on the upper surface of the main ring, a water storage pipe is fixedly penetrated through the upper surface of the main ring and located between each two adjacent placement holes, a bag pipe is fixedly connected to the bottom surface of the main ring and located below each placement hole, a power telescopic rod is arranged on the left side of the main ring, and the lower end of the power telescopic rod is fixedly connected to the base; the stirring device for nucleic acid extraction provided by the utility model is provided with the main ring and the water storage pipe, the power telescopic rod pulls the stirring structure to move to the inside of the water storage pipe, and when the stirring structure rotates, it rotates relative to the water inside the water storage pipe to complete cleaning, and then the sample in the next reagent tube can be stirred without pollution.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction, in particular to a stirring device for nucleic acid extraction. Background Art

[0002] Nucleic acid is the carrier of genetic information, the most important bioinformatics molecule, and the main object of molecular biology research. Therefore, nucleic acid extraction is crucial to molecular biology experimental technology. Genetic testing usually goes through the processes of sample collection, nucleic acid extraction, reagent preparation, amplification, and machine testing. The purpose of nucleic acid extraction is to decompose the protein in the sample so that the nucleic acid is exposed to the extract to facilitate PCR amplification reaction.

[0003] Based on current nucleic acid extraction technology, during the extraction process, the reagents and samples need to be fully mixed, so a stirring device is needed to assist in the mixing and prevent the reactants from solidifying during the mixing process. After the existing stirring device stirs the sample in the test tube, it is not convenient to clean the stirring structure, so the sample attached to the surface of the stirring structure is easy to contaminate the sample to be mixed next time. Therefore, a stirring device for nucleic acid extraction is proposed. Utility Model Content

[0004] In view of the problem that the existing stirring device is not convenient to clean the stirring structure after stirring the sample in the test tube, and the sample attached to the surface of the stirring structure is easy to contaminate the sample mixed next time, the utility model is proposed.

[0005] In order to solve the above technical problems, the utility model provides the following technical solutions: a stirring device for nucleic acid extraction, which includes a base, a main ring is rotatably penetrated on the upper surface of the base, a main shaft is rotatably penetrated on the upper surface of the base, the inner ring surface of the main ring is fixedly sleeved on the upper end of the main shaft, a plurality of placement holes are opened on the upper surface of the main ring, a water storage pipe is fixedly penetrated on the upper surface of the main ring and located between each two adjacent placement holes, a bag pipe is fixedly connected to the bottom surface of the main ring and located below each placement hole, a power telescopic rod is arranged on the left side of the main ring, the lower end of the power telescopic rod is fixedly connected to the base, a cross plate is fixedly connected to the upper end of the power telescopic rod, a power motor is fixedly connected to the bottom surface of the cross plate, a first gear is rotatably penetrated on the upper surface of the cross plate, the lower end of the first gear is fixedly connected to the output end of the power motor, a second gear is meshed on the right side of the first gear, a stirring structure is installed on the other end of the cross plate, the second gear is fixedly installed with the stirring structure, an intermittent motor is fixedly connected to the bottom surface of the base, and the lower end of the main shaft is fixedly connected to the output end of the intermittent motor.

[0006] As a preferred embodiment of the stirring device for nucleic acid extraction of the utility model, the stirring structure includes a sleeve shaft and a prism shaft, the cross plate is rotatably sleeved on the outer surface of the sleeve shaft, the sleeve shaft is slidably sleeved on the outer surface of the prism shaft, the second gear is fixedly sleeved on the outer surface of the sleeve shaft, one end of the sleeve shaft located below the cross plate is fixedly connected to an elastic stirring plate, and the lower end of the elastic stirring plate is fixedly connected to the lower end of the prism shaft.

[0007] As a preferred embodiment of the stirring device for nucleic acid extraction of the utility model, the prism shaft is coaxially arranged with the sleeve shaft, a ball is rotatably embedded at the lower end of the prism shaft, a plurality of elastic stirring plates are arranged, and the plurality of elastic stirring plates are distributed in a circular array around the axis of the prism shaft.

[0008] As a preferred embodiment of the stirring device for nucleic acid extraction of the utility model, the distance between the axis of the prism axis and the axis of the main shaft is equal to the distance between the axis of the placement hole and the axis of the main shaft, the distance between the axis of the placement hole and the axis of the main shaft is equal to the distance between the axis of the water storage pipe and the axis of the main shaft, and multiple placement holes and multiple water storage pipes are distributed in a circular array around the axis of the main shaft.

[0009] As a preferred embodiment of the stirring device for nucleic acid extraction described in the utility model, a concentric ring is arranged below the main ring, the main ring and the concentric ring are connected by a tension telescopic rod, a plug is slidably penetrated through the lower end of the water storage pipe, the lower end of the plug is fixedly connected to the concentric ring, a water collecting ring barrel is arranged below the concentric ring, the lower end of the water collecting ring barrel is fixedly connected to the upper surface of the base, and the main shaft is located on the inner ring side of the water collecting ring barrel.

[0010] As a preferred solution of the stirring device for nucleic acid extraction of the utility model, the inner ring surface of the bag tube is provided with a plurality of vertical grooves, and an elastic clamp is fixedly inserted in the interior of each vertical groove.

[0011] Beneficial effects of the utility model:

[0012] 1. By setting the main ring and the water storage pipe, after stirring the sample, the power telescopic rod pulls the stirring structure out of the reagent tube, and then the intermittent motor drives the main ring to rotate a certain angle through the main shaft, so that the adjacent water storage pipe moves to the bottom of the stirring structure, and then the power telescopic rod pulls the stirring structure to move to the inside of the water storage pipe. When the stirring structure rotates, it rotates relative to the water inside the water storage pipe to complete the cleaning, and then the sample in the next reagent tube can be stirred without pollution;

[0013] 2. By setting a plug, a concentric ring and a tension telescopic rod, the tension telescopic rod drives the plug to seal the lower end of the water storage pipe by pulling the concentric ring, so that the water storage pipe can store water. When the water in the water storage pipe needs to be replaced, the concentric ring is pushed to drive the plug to separate from the water storage pipe, so that water can flow from the water storage pipe to the water collecting ring barrel. After loosening the concentric ring, the tension telescopic rod pulls the plug to re-seal the water storage pipe, and new water can be re-added into the water storage pipe. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following briefly introduces the drawings required for the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0015] Figure 1 It is a schematic diagram of the structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the connection between the elastic stirring plate and the prism shaft of the utility model;

[0017] Figure 3 This is a schematic diagram of the connection between the plug and the water storage pipe of the utility model;

[0018] Figure 4 It is a schematic diagram of the internal structure of the bag tube of the utility model.

[0019] Description of reference numerals:

[0020] 1. Base; 2. Main ring; 3. Stirring structure; 301. Sleeve shaft; 302. Prismatic shaft; 303. Elastic stirring plate; 4. Main shaft; 5. Power motor; 6. Placement hole; 7. Horizontal plate; 8. First gear; 9. Second gear; 10. Intermittent motor; 11. Power telescopic rod; 12. Bag pipe; 13. Water storage pipe; 14. Ball; 15. Concentric ring; 16. Tension telescopic rod; 17. Plug; 18. Water collecting ring barrel; 19. Vertical groove; 20. Elastic splint. DETAILED DESCRIPTION

[0021] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0022] Reference Figure 1-4, is an embodiment of the utility model, which provides a stirring device for nucleic acid extraction, the stirring device for nucleic acid extraction includes a base 1, the upper surface of the base 1 is rotatably penetrated by a main ring 2, the upper surface of the base 1 is rotatably penetrated by a main shaft 4, the inner ring surface of the main ring 2 is fixedly sleeved on the upper end of the main shaft 4, a plurality of placement holes 6 are opened on the upper surface of the main ring 2, a water storage pipe 13 is fixedly penetrated on the upper surface of the main ring 2 and located between each two adjacent placement holes 6, the water storage pipe 13 stores water, a concentric ring 15 is arranged below the main ring 2, the main ring 2 and the concentric ring 15 are connected by a tension telescopic rod 16, the lower end of the water storage pipe 13 is slidably penetrated by a plug 17, the lower end of the plug 17 is fixedly connected to the concentric ring 15, the tension telescopic rod 16 has a pulling plug 17 In order to block the water storage pipe 13, a water collecting ring barrel 18 is arranged below the concentric ring 15, and the lower end of the water collecting ring barrel 18 is fixedly connected to the upper surface of the base 1, and the main shaft 4 is located on the inner ring side of the water collecting ring barrel 18. After the plug 17 is separated from the water storage pipe 13, the water in the water storage pipe 13 flows into the water collecting ring barrel 18. After the plug 17 blocks the water storage pipe 13 again, clean water can be added to the water storage pipe 13. The bottom surface of the main ring 2 and below each placement hole 6 are fixedly connected with a bag tube 12. The inner ring surface of the bag tube 12 is provided with a plurality of vertical grooves 19, and an elastic splint 20 is fixedly inserted inside each vertical groove 19. The reagent tube is inserted into the bag tube 12 through the placement hole 6, and the elastic splint 20 clamps the reagent tube through elastic deformation.

[0023] A power telescopic rod 11 is provided on the left side of the main ring 2, the lower end of the power telescopic rod 11 is fixedly connected to the base 1, the upper end of the power telescopic rod 11 is fixedly connected to a horizontal plate 7, the bottom surface of the horizontal plate 7 is fixedly connected to a power motor 5, a first gear 8 is rotatably passed through the upper surface of the horizontal plate 7, the lower end of the first gear 8 is fixedly connected to the output end of the power motor 5, a second gear 9 is meshed with the right side of the first gear 8, a stirring structure 3 is installed at the other end of the horizontal plate 7, the second gear 9 is fixedly installed with the stirring structure 3, and the bottom surface of the base 1 is fixedly connected to the first gear 8. The intermittent motor 10 is connected. Each time the intermittent motor 10 drives the main ring 2 to rotate once, the main ring 2 drives the water storage pipe 13 and the placement hole 6 to move alternately to the bottom of the stirring structure 3. The lower end of the main shaft 4 is fixedly connected to the output end of the intermittent motor 10. The stirring structure 3 includes a sleeve shaft 301 and a prism shaft 302. The cross plate 7 is rotatably sleeved on the outer surface of the sleeve shaft 301. The sleeve shaft 301 is slidably sleeved on the outer surface of the prism shaft 302. The distance between the axis of the prism shaft 302 and the axis of the main shaft 4 is equal to the distance between the axis of the placement hole 6 and the axis of the main shaft 4. The distance between the axis of the placement hole 6 and the axis of the main shaft 4 is equal to the distance between the axis of the water storage pipe 13 and the axis of the main shaft 4. The plurality of placement holes 6 and the plurality of water storage pipes 13 are arranged in a circular array around the axis of the main shaft 4. The second gear 9 is fixedly sleeved on the outer surface of the sleeve shaft 301. The end of the sleeve shaft 301 located below the horizontal plate 7 is fixedly connected with an elastic stirring plate 303. The lower end of the elastic stirring plate 303 is fixedly connected with the lower end of the prism shaft 302. When the power telescopic rod 5 drives the prism shaft 302 to enter the reagent tube and the water storage pipe 13, the prism shaft 302 After contacting the lower end of the reagent tube and the lower end of the water storage tube 13, the sleeve shaft 301 continues to move downward to push the elastic stirring plate 303 to bend and deform, thereby increasing the stirring effect of the elastic stirring plate 303. The prism shaft 302 is coaxially arranged with the sleeve shaft 301, and the lower end of the prism shaft 302 is rotatably inlaid with a ball 14. The ball 14 reduces the friction of the prism shaft 302 on the reagent tube and the water storage tube 13 when the prism shaft 302 contacts with the reagent tube and the water storage tube 13. A plurality of elastic stirring plates 303 are arranged, and the plurality of elastic stirring plates 303 are distributed in a circular array around the axis of the prism shaft 302.

[0024] During use, the reagent tube to be stirred is inserted into the bag tube 12, and the elastic clamping plate 20 in the bag tube 12 squeezes and clamps the reagent tube through elastic deformation, and the intermittent motor 10 drives the main ring 2 to rotate, so that the reagent tube rotates to below the prism shaft 302, and the power telescopic rod 11 pulls the prism shaft 302 downward to enter the reagent tube. After the prism shaft 302 contacts the lower end of the reagent tube, the sleeve shaft 301 continues to move downward, and the elastic stirring plate 303 is bent in an arc shape. The power motor 5 drives the sleeve shaft 301 and the prism shaft 302 to rotate through the engagement of the first gear 8 and the second gear 9, and the prism shaft 302 drives the arc-bent elastic stirring plate 303 to stir the sample in the reagent tube. After stirring, the power telescopic rod 11 pushes the cross plate 7 to move upward, with The movable prism shaft 302 is separated from the reagent tube, and then the intermittent motor 10 controls the main ring 2 to drive the adjacent water storage pipe 13 to rotate to the bottom of the prism shaft 302. After the power telescopic rod 11 pulls the prism shaft 302 into the water storage pipe 13, the sleeve shaft 301 drives the prism shaft 302 and the elastic stirring plate 303 to rotate. The elastic stirring plate 303 and the prism shaft 302 complete the cleaning by relative rotation relative to the water in the water storage pipe 13. The power telescopic rod 11 pulls the prism shaft 302 out of the water storage pipe 13, and the intermittent motor 10 controls the next reagent tube to move to the bottom of the prism shaft 302 for stirring. During the reciprocating extension and retraction of the power telescopic rod 11 and the intermittent rotation of the intermittent motor 10, the stirring structure 3 stirs the reagent tube and then can enter the water storage pipe 13 for cleaning.

[0025] It should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A stirring device for nucleic acid extraction, comprising a base (1), characterized in that: The upper surface of the base (1) is rotatably penetrated by a main ring (2), the upper surface of the base (1) is rotatably penetrated by a main shaft (4), the inner ring surface of the main ring (2) is fixedly sleeved on the upper end of the main shaft (4), the upper surface of the main ring (2) is provided with a plurality of placement holes (6), the upper surface of the main ring (2) and between each two adjacent placement holes (6) are fixedly penetrated by a water storage pipe (13), the bottom surface of the main ring (2) and below each placement hole (6) are fixedly connected to a bag pipe (12), a power telescopic rod (11) is provided on the left side of the main ring (2), the lower end of the power telescopic rod (11) is fixedly connected to the base (1), and the power telescopic rod (11) is rotatably penetrated by a main ring (2) and the lower end of the power telescopic rod (11) is fixedly connected to the base (1), and the power telescopic rod (11) is rotatably penetrated by a main shaft (4), and the inner ring surface of the main ring (2) is fixedly sleeved on the upper end of the main ring (2) and the upper end of the power telescopic rod (11) is fixedly connected to the upper end of the main ring (1). The upper end of the telescopic rod (11) is fixedly connected to a horizontal plate (7), the bottom surface of the horizontal plate (7) is fixedly connected to a power motor (5), a first gear (8) is rotatably passed through the upper surface of the horizontal plate (7), the lower end of the first gear (8) is fixedly connected to an output end of the power motor (5), a second gear (9) is meshed on the right side of the first gear (8), a stirring structure (3) is installed at the other end of the horizontal plate (7), the second gear (9) is fixedly installed with the stirring structure (3), an intermittent motor (10) is fixedly connected to the bottom surface of the base (1), and the lower end of the main shaft (4) is fixedly connected to the output end of the intermittent motor (10).

2. A stirring device for nucleic acid extraction according to claim 1, characterized in that: The stirring structure (3) comprises a sleeve shaft (301) and a prism shaft (302); the transverse plate (7) is rotatably sleeved on the outer surface of the sleeve shaft (301); the sleeve shaft (301) is slidably sleeved on the outer surface of the prism shaft (302); the second gear (9) is fixedly sleeved on the outer surface of the sleeve shaft (301); one end of the sleeve shaft (301) located below the transverse plate (7) is fixedly connected to an elastic stirring plate (303); and the lower end of the elastic stirring plate (303) is fixedly connected to the lower end of the prism shaft (302).

3. A stirring device for nucleic acid extraction according to claim 2, characterized in that: The prism shaft (302) is coaxially arranged with the sleeve shaft (301), a ball (14) is rotatably embedded in the lower end of the prism shaft (302), a plurality of elastic stirring plates (303) are arranged, and the plurality of elastic stirring plates (303) are distributed in a circular array around the axis of the prism shaft (302).

4. A stirring device for nucleic acid extraction according to claim 2, characterized in that: The distance between the axis of the prism axis (302) and the axis of the main axis (4) is equal to the distance between the axis of the placement hole (6) and the axis of the main axis (4), the distance between the axis of the placement hole (6) and the axis of the main axis (4) is equal to the distance between the axis of the water storage pipe (13) and the axis of the main axis (4), and the plurality of placement holes (6) and the plurality of water storage pipes (13) are distributed in a circular array around the axis of the main axis (4).

5. A stirring device for nucleic acid extraction according to claim 1, characterized in that: A concentric ring (15) is arranged below the main ring (2), the main ring (2) and the concentric ring (15) are connected via a tension telescopic rod (16), a plug (17) is slidably passed through the lower end of the water storage pipe (13), the lower end of the plug (17) is fixedly connected to the concentric ring (15), a water collecting ring barrel (18) is arranged below the concentric ring (15), the lower end of the water collecting ring barrel (18) is fixedly connected to the upper surface of the base (1), and the main shaft (4) is located on the inner ring side of the water collecting ring barrel (18).

6. A stirring device for nucleic acid extraction according to claim 1, characterized in that: The inner annular surface of the bag tube (12) is provided with a plurality of vertical grooves (19), and an elastic clamping plate (20) is fixedly inserted into the interior of each vertical groove (19).

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