A cell filtration device for avoiding contamination

Abstract

The utility model relates to the field of cell filtration, in particular to a cell filtration device for avoiding pollution, comprising: a filter housing, a connection cover is installed on the top of the filter housing, a connection head is installed on the top of the connection cover; a filter mechanism is installed inside the filter housing and can discharge the air inside the filter housing; by utilizing the cooperation between the filter mechanism and the connection cover, when the connection cover is installed, the filter is driven to squeeze a pressure plate, the inert gas inside a piston plate is injected into the top of an inner cavity of the filter housing through a connecting pipe, and the inert gas is squeezed against the air inside the filter housing by the descent of the filter, and the air inside the filter housing is discharged through an exhaust pipe, so that the air inside the filter housing can be discharged through the installation trend of the connection cover, and the situation that the air inside the filter housing still pollutes the cells is avoided.

Description

Technical Field

[0001] The utility model relates to the field of cell filtration, in particular to a cell filtration device for avoiding pollution. Background Art

[0002] At present, there are abundant sources of materials for obtaining stem cells. Umbilical cord mesenchymal stem cells have been separated from bone marrow, umbilical cord, placenta, adipose tissue, liver tissue and umbilical cord blood, but the amount obtained is limited and the material is difficult to obtain. Recent studies have reported that placental tissue has become a hot topic of research by many researchers due to its advantages of easy material collection and more stem cells obtained. After the placental tissue is processed by certain experimental methods in the early stage and filtered, a large number of various types of stem cells can be obtained for research and application;

[0003] After searching, it is found that the authorization announcement number is: CN214019488U, which is a placental tissue stem cell filtration device that prevents bacterial contamination. Although this device has the advantage of reducing its contact with the external air and reducing pollution, the device can only be isolated from the external air, and the air inside the device will still cause contamination to the cells.

[0004] Therefore, a cell filtration device that avoids contamination is proposed to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide a cell filtering device that avoids contamination in order to solve the above-mentioned problem, thereby improving the problem that the device can only be isolated from the external air, and the air inside the device can still cause contamination to the cells.

[0006] The utility model achieves the above-mentioned purpose through the following technical scheme, a cell filtering device for avoiding pollution, comprising: a filter housing, a connecting cover is installed on the top of the filter housing, and a connecting head is installed on the top of the connecting cover; a filtering mechanism, the filtering mechanism is installed inside the filter housing and can discharge the air inside the filter housing; wherein the filtering mechanism comprises two exhaust pipes installed on the front and rear sides of the filter housing, a first one-way valve is installed on the surface of the exhaust pipe, a filter is installed on the bottom of the connecting cover, an air storage cylinder is installed on the bottom of the inner cavity of the filter housing, and an air injection component is installed through the surface of the air storage cylinder.

[0007] Preferably, the gas injection assembly includes a piston plate slidably mounted inside the gas cylinder, a pressure plate is provided on the top of the gas cylinder, a connecting rod is fixedly mounted between the piston plate and the pressure plate, a return spring is fixedly mounted on the top of the piston plate, the top of the return spring is fixedly connected to the top of the inner cavity of the gas cylinder, the connecting rod passes through the gas cylinder and is slidably connected thereto, connecting pipes are installed at the bottoms of both sides of the gas cylinder, the end of the connecting pipe away from the gas cylinder passes through the filter shell and extends to the top of the inner cavity of the filter shell, and a second one-way valve is installed on the surface of the connecting pipe.

[0008] Preferably, the filter includes an inner filter cartridge installed at the bottom of the connecting cover, and fixing plates are fixedly installed around the outer side wall of the inner filter cartridge. The inner filter cartridge is fixedly installed with an outer filter cartridge via the fixing plates, and the pore size of the outer filter cartridge surface is smaller than the pore size of the inner filter cartridge surface, and the diameter of the outer filter cartridge is smaller than the inner diameter of the filter housing.

[0009] Preferably, a gas filling pipe is installed at the bottom of one side of the gas storage cylinder, one end of the gas filling pipe away from the gas storage cylinder passes through the filter housing and extends to the outside thereof, and a valve is installed on the surface of the gas filling pipe located outside the filter housing.

[0010] Preferably, a protective shell is installed on the surface of the connecting pipe and the second one-way valve located outside the filter housing, and one side of the protective shell is fixedly connected to the filter housing.

[0011] Preferably, bolts are installed through both sides of the top of the inner filter cartridge, and the inner filter cartridge is fixedly connected to the connecting cover by the bolts.

[0012] Preferably, a sealing ring is installed at the penetration point between the air storage cylinder and the connecting rod, and the sealing ring is made of a rubber component.

[0013] The beneficial effects of the utility model are:

[0014] 1. By utilizing the cooperation between the filter mechanism and the connection cover, the filter is driven to squeeze the pressure plate when the connection cover is installed, and the inert gas inside the piston plate is injected into the top of the inner cavity of the filter housing through the connecting pipe. The inert gas squeezes the air inside the filter housing through the descent of the filter, and the air inside the filter housing is discharged through the exhaust pipe, which plays a role in being able to discharge the air inside the filter housing through the installation trend of the connection cover, avoiding the situation where the air inside the filter housing still causes pollution to the cells;

[0015] 2. Through the setting of the filter, the connector on the top of the connecting cover is used to connect to the cell bottle, and the cells are introduced into the interior of the filter. When the cells enter the interior of the inner filter cartridge, the first filtration is performed. At the same time, through the flow characteristics of the cell culture fluid, it passes through the outer filter cartridge for the second filtration and then flows into the interior of the filter shell, which makes it easy to filter the cells multiple times and can cooperate with the gas injection component to prevent the cells from excessive contact with the air during filtration, which may cause the cells to be contaminated during filtration. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model;

[0017] Figure 2 It is a cross-sectional structural schematic diagram of the utility model;

[0018] Figure 3 It is a schematic diagram of the cross-sectional structure of the gas storage cylinder of the utility model;

[0019] Figure 4 This is a schematic diagram of the filter structure of the utility model.

[0020] In the figure: 10, filter housing; 11, connecting cover; 12, connecting head; 20, filtering mechanism; 21, exhaust pipe; 22, filter; 221, inner filter cartridge; 222, fixing plate; 223, outer filter cartridge; 23, air storage cylinder; 24, air injection assembly; 241, piston plate; 242, pressure plate; 243, connecting rod; 244, return spring; 245, connecting pipe; 246, second one-way valve; 247, air filling pipe; 248, protective shell. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] When implementing: Figure 1-4 As shown, a cell filtration device for avoiding contamination comprises:

[0023] A filter housing 10, a connection cover 11 is installed on the top of the filter housing 10, and a connector 12 is installed on the top of the connection cover 11;

[0024] The filter mechanism 20 is installed inside the filter housing 10 and can discharge the air inside the filter housing 10;

[0025] The filter mechanism 20 includes two exhaust pipes 21 connected and installed on the front and rear sides of the filter housing 10, a first one-way valve is installed on the surface of the exhaust pipe 21, a filter 22 is installed at the bottom of the connection cover 11, an air storage cylinder 23 is installed at the bottom of the inner cavity of the filter housing 10, and an air injection assembly 24 is installed on the surface of the air storage cylinder 23; the air injection assembly 24 includes a piston plate 241 slidably installed inside the air storage cylinder 23, a pressure plate 242 is provided on the top of the air storage cylinder 23, and the piston plate 241 and the pressure plate 24 2, a connecting rod 243 is fixedly installed between the piston plate 241, a return spring 244 is fixedly installed on the top of the piston plate 241, the top of the return spring 244 is fixedly connected to the top of the inner cavity of the air storage cylinder 23, the connecting rod 243 penetrates the air storage cylinder 23 and is slidably connected thereto, and the bottoms of both sides of the air storage cylinder 23 are connected and installed with connecting pipes 245, one end of the connecting pipe 245 away from the air storage cylinder 23 penetrates the filter housing 10 and extends to the top of the inner cavity of the filter housing 10, and a second one-way valve 246 is installed on the surface of the connecting pipe 245;

[0026] In this embodiment, when the connection cover 11 is installed, the filter 22 is driven to squeeze the pressure plate 242, and the inert gas inside the piston plate 241 is injected into the top of the inner cavity of the filter housing 10 through the connecting pipe 245. The inert gas squeezes the air inside the filter housing 10 through the descending of the filter 22, and the air inside the filter housing 10 is discharged through the exhaust pipe 21.

[0027] In this embodiment, a gas filling pipe 247 is connected and installed at the bottom of one side of the gas storage cylinder 23. One end of the gas filling pipe 247 away from the gas storage cylinder 23 passes through the filter housing 10 and extends to the outside thereof. A valve is installed on the surface of the gas filling pipe 247 located outside the filter housing 10; after the inert gas inside the gas storage cylinder 23 is used up, the external inert gas can be injected into the gas storage cylinder 23, so as to inflate the gas storage cylinder 23.

[0028] In this embodiment, a protective shell 248 is installed on the surface of the connecting pipe 245 and the second one-way valve 246 outside the filter housing 10, and one side of the protective shell 248 is fixedly connected to the filter housing 10; a sealing ring is installed at the penetration point of the air storage cylinder 23 and the connecting rod 243, and the material of the sealing ring is a rubber component.

[0029] like Figure 2 and Figure 4 As shown, the filter 22 includes an inner filter cartridge 221 installed at the bottom of the connection cover 11, and a fixing plate 222 is fixedly installed around the outer side wall of the inner filter cartridge 221. The inner filter cartridge 221 is fixedly installed with an outer filter cartridge 223 through the fixing plate 222. The pore size of the surface of the outer filter cartridge 223 is smaller than the pore size of the surface of the inner filter cartridge 221, and the diameter of the outer filter cartridge 223 is smaller than the inner diameter of the filter housing 10.

[0030] In this embodiment, the connector 12 on the top of the connection cover 11 is used to connect with the cell bottle, and the cells are introduced into the filter 22. When the cells enter the inner filter cartridge 221, the cells are filtered for the first time. At the same time, due to the flow characteristics of the cell culture fluid, the cells are filtered for the second time through the outer filter cartridge 223 and then flow into the filter housing 10.

[0031] In this embodiment, bolts are installed through both sides of the top of the inner filter cartridge 221, and the inner filter cartridge 221 is fixedly connected to the connecting cover 11 by bolts; this makes it easy for the staff to separate the connecting cover 11 and the filter 22, so as to clean the filter 22 after cell filtration to avoid affecting subsequent filtration.

[0032] When the utility model is in use, the connector 12 on the top of the connecting cover 11 is connected to the cell bottle, and the connector 12 is connected to the cell bottle. When the connecting cover 11 is installed, the filter 22 is driven to squeeze the pressure plate 242, and the inert gas inside the piston plate 241 is injected into the top of the inner cavity of the filter housing 10 through the connecting pipe 245. The inert gas squeezes the air inside the filter housing 10 through the descent of the filter 22, and the air inside the filter housing 10 is discharged through the exhaust pipe 21; at the same time, the cells are introduced into the interior of the filter 22, and when the cells enter the interior of the inner filter cartridge 221, the first filtration is performed, and then according to the flow characteristics of the cell culture fluid, the cells are filtered for the second time through the outer filter cartridge 223 and then flow into the interior of the filter housing 10.

[0033] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A cell filtration device for avoiding contamination, characterized in that: include: A filter housing (10), wherein a connection cover (11) is installed on the top of the filter housing (10), and a connector (12) is installed on the top of the connection cover (11); A filter mechanism (20), wherein the filter mechanism (20) is installed inside the filter housing (10) and can discharge the air inside the filter housing (10); The filtering mechanism (20) comprises two exhaust pipes (21) connected and installed on the front and rear sides of the filter housing (10); a first one-way valve is installed on the surface of the exhaust pipe (21); a filter (22) is installed at the bottom of the connecting cover (11); an air storage cylinder (23) is installed at the bottom of the inner cavity of the filter housing (10); and an air injection assembly (24) is installed through the surface of the air storage cylinder (23).

2. A cell filtration device for avoiding contamination according to claim 1, characterized in that: The gas injection assembly (24) comprises a piston plate (241) slidably mounted inside the gas cylinder (23); a pressure plate (242) is arranged at the top of the gas cylinder (23); a connecting rod (243) is fixedly mounted between the piston plate (241) and the pressure plate (242); a return spring (244) is fixedly mounted on the top of the piston plate (241); the top end of the return spring (244) is fixedly connected to the top of the inner cavity of the gas cylinder (23); the connecting rod (243) penetrates the gas cylinder (23) and is slidably connected thereto; connecting pipes (245) are connected and mounted at the bottom of both sides of the gas cylinder (23); the end of the connecting pipe (245) away from the gas cylinder (23) penetrates the filter housing (10) and extends to the top of the inner cavity of the filter housing (10); a second one-way valve (246) is mounted on the surface of the connecting pipe (245).

3. A cell filtration device for avoiding contamination according to claim 2, characterized in that: The filter (22) comprises an inner filter cartridge (221) mounted on the bottom of the connection cover (11); fixing plates (222) are fixedly mounted on all four sides of the outer side wall of the inner filter cartridge (221); an outer filter cartridge (223) is fixedly mounted on the inner filter cartridge (221) via the fixing plates (222); the pore size of the surface of the outer filter cartridge (223) is smaller than the pore size of the surface of the inner filter cartridge (221); and the diameter of the outer filter cartridge (223) is smaller than the inner diameter of the filter housing (10).

4. A cell filtration device for avoiding contamination according to claim 2, characterized in that: A gas filling pipe (247) is installed at the bottom of one side of the gas storage cylinder (23), and one end of the gas filling pipe (247) away from the gas storage cylinder (23) passes through the filter housing (10) and extends to the outside thereof. A valve is installed on the surface of the gas filling pipe (247) located outside the filter housing (10).

5. A cell filtration device for avoiding contamination according to claim 2, characterized in that: A protective shell (248) is installed on the surface of the connecting pipe (245) and the second one-way valve (246) located outside the filter housing (10), and one side of the protective shell (248) is fixedly connected to the filter housing (10).

6. A cell filtration device for avoiding contamination according to claim 3, characterized in that: Bolts are installed through both sides of the top of the inner filter cartridge (221), and the inner filter cartridge (221) is fixedly connected to the connection cover (11) via the bolts.

7. A cell filtration device for avoiding contamination according to claim 2, characterized in that: A sealing ring is installed at the penetration point between the air storage cylinder (23) and the connecting rod (243), and the sealing ring is made of a rubber component.

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