A PCR reaction tube capable of effectively preventing contamination
By designing a PCR reaction tube including a test tube, an isolation chamber and a sealing cover, the problem of amplification products escaping during sampling of the PCR reaction tube is solved, and effective pollution prevention and experimental efficiency are improved.
Patent Information
- Application Number
- CN202420857030.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-24
- Publication Date
- 2025-04-01
- Estimated Expiration
- 2034-04-24
AI Technical Summary
The existing PCR reaction tubes are prone to cause the amplification product to escape in the form of aerosol during sampling, causing laboratory contamination and affecting subsequent experimental results.
A PCR reaction tube including a test tube, an isolation chamber and a sealing cover was designed. By setting a limit groove and a snap ring on the top of the inner wall of the test tube, a snap ring is installed on the side wall of the bottom end of the isolation chamber, and fixing the mounting blocks and slots on both sides of the bottom of the sealing cover and both sides of the top of the test tube to ensure that the communication ports of the sealing cover and the isolation chamber are sealed by the sampling gun during sampling to prevent the amplification product from escaping.
It effectively prevents contamination when the test tube is opened, improves experimental efficiency, simplifies experimental steps, and ensures the accuracy of experimental results.
Smart Images
Figure CN222700300U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of PCR reaction tubes, in particular to a PCR reaction tube which can effectively prevent pollution. Background Art
[0002] PCR reaction tubes play a vital role in PCR technology. PCR, or polymerase chain reaction, is a method for rapidly amplifying specific genes or DNA sequences in vitro. PCR reaction tubes are usually made of medical-grade polypropylene, with an integrated single-tube design and good sealing to prevent contamination and easy opening. This tube can be autoclaved, the flat cap design is easy to write or label, and color PCR tubes can be customized individually.
[0003] In general, PCR reaction tubes are an important part of PCR technology, and their quality and design directly affect the results of PCR experiments. With the continuous development of technology, more innovative designs may appear in the future, providing more efficient and accurate tools for scientific research.
[0004] In existing PCR reaction tubes, when the PCR tube cover is opened for sampling in PCR experiments, the amplification products in the tube (i.e., a large number of replicated DNA fragments) may escape in the form of aerosols and remain suspended in the air. These aerosols containing DNA fragments can diffuse in the laboratory and settle on other experimental equipment and surfaces such as the laboratory bench, PCR instruments, and pipettes. The DNA fragments in these aerosols are brought into subsequent PCR experiments, affecting the subsequent experimental results.
[0005] Therefore, it is necessary to provide a PCR reaction tube that can effectively prevent contamination to solve the above technical problems. Utility Model Content
[0006] The utility model aims to provide a PCR reaction tube which can effectively prevent pollution, and has the effects of avoiding pollution caused when the test tube is opened, improving experimental efficiency and simplifying experimental steps.
[0007] The above-mentioned technical purpose of the utility model is achieved through the following technical solutions: a PCR reaction tube that can effectively prevent contamination, comprising a test tube, a limiting groove is provided on the top of the inner wall of the test tube, an isolation chamber is rotatably arranged on the top of the test tube, a clamping ring is fixedly installed on the bottom side wall of the isolation chamber, the clamping ring is arranged inside the limiting groove, a sealing plate is fixedly installed on the top and bottom of the isolation chamber, a sealing cover is rotatably connected to the top of the sealing plate near the top of the isolation chamber, a clamping groove is provided on both sides of the top and bottom of the isolation chamber, clamping blocks are fixedly installed on both sides of the bottom of the sealing cover and both sides of the top of the test tube, the clamping block is adapted to the clamping groove, a first sampling port is provided on one side of the top of the sealing cover, a connecting port is provided on the side of the two sealing plates away from the first sampling port, and a second sampling port is provided on the side of the top of the test tube away from the connecting port.
[0008] By adopting the above technical solution, the contamination caused when the test tube is opened can be avoided, the experimental efficiency can be improved, and the experimental steps can be simplified.
[0009] The utility model is further configured as follows: the side walls of the isolation chamber and the test tube are both provided with anti-slip grooves.
[0010] By adopting the above technical solution, the friction of the side wall of the test tube is increased by the anti-slip pattern, thereby improving the grip feeling.
[0011] The utility model is further configured as follows: paddles are fixedly mounted on both sides of the top of the sealing cover.
[0012] By adopting the above technical solution, when rotating the sealing cover, it is more convenient and labor-saving to rotate it through the paddle, and the paddle is also convenient for taking the test tube.
[0013] The utility model is further configured as follows: a marking groove is provided on the top of the sealing cover.
[0014] By adopting the above technical solution, the capacity and other information of the test tube are engraved on the bottom of the identification groove, which is convenient for selecting a suitable test tube for use. At the same time, the label can be placed at the bottom of the identification groove, so that the label is not easily wiped off by friction and can be retained for a long time.
[0015] The utility model is further configured as follows: silicone sheets are fixedly mounted on the bottom of the sealing cover, the top of the test tube and the two sealing plates, and the plurality of silicone sheets are respectively in contact with the first sampling port, the second sampling port and the two connecting ports.
[0016] By adopting the above technical solution, the sealing performance of the device is increased by sealing the connecting openings and the like with the silicone sheets.
[0017] The utility model is further configured as follows: the test tube, the isolation chamber and the sealing cover are all made of transparent plastic.
[0018] By adopting the above technical solution, it is convenient to directly observe the inside of the test tube through the transparent plastic in the laboratory.
[0019] In summary, the utility model has the following beneficial effects: when using a PCR reaction tube, in order to prevent the amplification product (i.e., a large number of replicated DNA fragments) in the tube from escaping in the form of aerosol and being suspended in the air, which will affect the subsequent experimental results, when sampling, first rotate the sealing cover to connect the first sampling port with the connecting port on the top of the sealing plate, then insert the gun head of the sampling gun into the isolation chamber through the first sampling port on the sealing cover, and then rotate the isolation chamber to connect the connecting port at the bottom of the isolation chamber with the second sampling port on the top of the test tube, and then rotate the isolation chamber to connect the connecting port at the bottom of the isolation chamber with the second sampling port on the top of the test tube. Sampling is performed by entering the test tube through the second sampling port. When sampling, the connecting port on the top of the isolation chamber and the first sampling port are blocked by the sampling gun to ensure sealing, so that the amplification product in the tube cannot leak out from the inside of the test tube, which can effectively prevent contamination. When rotating the isolation chamber, the limit groove and the clamping ring assist the rotation of the isolation chamber, and the first sampling tube, the second sampling tube and the two connecting ports can be easily aligned through the engagement of the clamping block and the clamping groove, which is easy to use. The device can avoid contamination caused by opening the test tube, improve experimental efficiency, and simplify experimental steps. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;
[0021] Figure 2 It is a three-dimensional cross-sectional structural schematic diagram of the utility model;
[0022] Figure 3 This is a schematic diagram of the main cross-sectional structure of the utility model;
[0023] Figure 4 It is a schematic diagram of the three-dimensional unfolded structure of the utility model;
[0024] Figure 5 It is a schematic diagram of the unfolded structure of the three-dimensional section of the utility model.
[0025] In the figure: 1. test tube; 2. isolation chamber; 3. sealing cover; 4. card slot; 5. card block; 6. first sampling port; 7. limit slot; 8. snap ring; 9. pick; 10. identification slot; 11. anti-slip groove; 12. silicone sheet; 13. connecting port; 14. second sampling port; 15. sealing plate. DETAILED DESCRIPTION
[0026] The present invention will be further described below with reference to the accompanying drawings in the embodiments of the present invention.
[0027] See also Figures 1 to 5 In an embodiment of the utility model, a PCR reaction tube that can effectively prevent pollution comprises a test tube 1, a limiting groove 7 is provided on the top of the inner wall of the test tube 1, an isolation chamber 2 is rotatably provided on the top of the test tube 1, a clamping ring 8 is fixedly installed on the bottom side wall of the isolation chamber 2, and the clamping ring 8 is arranged inside the limiting groove 7, a sealing plate 15 is fixedly installed on the top and bottom of the isolation chamber 2, a sealing cover 3 is rotatably connected to the top of the sealing plate 15 near the top of the isolation chamber 2, a clamping groove 4 is provided on both sides of the top and bottom of the isolation chamber 2, a clamping block 5 is fixedly installed on both sides of the bottom of the sealing cover 3 and both sides of the top of the test tube 1, and the clamping block 5 is adapted to the clamping groove 4, a first sampling port 6 is provided on one side of the top of the sealing cover 3, a connecting port 13 is provided on the side of the two sealing plates 15 away from the first sampling port 6, and a second sampling port 14 is provided on the side of the top of the test tube 1 away from the connecting port 13; when using the PCR reaction tube, in order to avoid the amplification product (i.e., a large number of replicated DNA fragments) in the tube ) escapes in the form of aerosol and is suspended in the air, which will affect the subsequent experimental results. When sampling, first rotate the sealing cover 3 to connect the first sampling with the connecting port 13 on the top of the sealing plate 15, and then insert the gun head of the sampling gun into the isolation chamber 2 through the first sampling port 6 on the sealing cover 3, and then rotate the isolation chamber to make the connecting port 13 at the bottom of the isolation chamber dock with the second sampling port 14 on the top of the test tube 1, and the gun head of the sampling gun enters the test tube 1 through the second sampling port 14 to take samples. When sampling, the isolation chamber The connecting port 13 and the first sampling port 6 at the top of the test tube 1 are blocked by the sampling gun to ensure sealing, so that the amplification product in the tube cannot leak out from the inside of the test tube 1, which can effectively prevent contamination. When the isolation chamber 2 is rotated, the limit groove 7 and the clamping ring 8 assist the rotation of the isolation chamber 2, and the first sampling tube, the second sampling tube and the two connecting ports 13 can be easily aligned through the engagement of the clamping block 5 and the clamping groove 4, which is convenient for use. The device can avoid contamination caused by opening the test tube 1, improve experimental efficiency, and simplify experimental steps.
[0028] In this embodiment, preferably, the side walls of the isolation chamber 2 and the test tube 1 are both provided with anti-skid patterns 11; the anti-skid patterns 11 are used to increase the friction of the side walls of the test tube 1 and enhance the gripping feeling.
[0029] In this embodiment, preferably, paddles 9 are fixedly installed on both sides of the top of the sealing cover 3; when the sealing cover 3 is rotated, it is more convenient and labor-saving to rotate through the paddles 9, and the paddles 9 are convenient for taking the test tube 1.
[0030] In this embodiment, preferably, an identification groove 10 is opened on the top of the sealing cover 3; the capacity and other information of the test tube 1 are engraved on the bottom of the identification groove 10, so that it is convenient to select a suitable test tube 1 for use. At the same time, the mark can be placed at the bottom of the identification groove 10, so that the mark is not easily wiped off by friction and can be retained for a long time.
[0031] In this embodiment, preferably, silicone sheets 12 are fixedly installed on the bottom of the sealing cover 3, the top of the test tube 1 and the two sealing plates 15, and the multiple silicone sheets 12 are respectively in contact with the first sampling port 6, the second sampling port 14 and the two connecting ports 13; the sealing performance of the device is increased by sealing the connecting ports 13 etc. with each silicone sheet 12.
[0032] In this embodiment, preferably, the test tube 1, the isolation chamber 2 and the sealing cover 3 are all made of transparent plastic; it is convenient to directly observe the inside of the test tube 1 through the transparent plastic in the laboratory.
[0033] Working principle: When using a PCR reaction tube, in order to prevent the amplified product (i.e., a large number of replicated DNA fragments) in the tube from escaping in the form of aerosol and being suspended in the air, which will affect the subsequent experimental results, when sampling, first rotate the sealing cover 3 to connect the first sampling with the connecting port 13 on the top of the sealing plate 15, and then insert the gun head of the sampling gun into the isolation chamber 2 through the first sampling port 6 on the sealing cover 3, and then rotate the isolation chamber to make the connecting port 13 at the bottom of the isolation chamber dock with the second sampling port 14 on the top of the test tube 1, and the gun head of the sampling gun passes through the second sampling port 14. Enter the test tube 1 to take samples. When taking samples, the connecting port 13 and the first sampling port 6 on the top of the isolation chamber 2 are blocked by the sampling gun to ensure sealing, so that the amplification product in the tube cannot leak out from the inside of the test tube 1, which can effectively prevent contamination. When the isolation chamber 2 is rotated, the limit groove 7 and the clamping ring 8 assist the rotation of the isolation chamber 2, and through the engagement of the clamping block 5 and the clamping groove 4, the first sampling tube, the second sampling tube and the two connecting ports 13 can be easily aligned, which is easy to use. The device can avoid the contamination caused by opening the test tube 1, improve the experimental efficiency, and simplify the experimental steps.
[0034] The above description is only a preferred embodiment of the present utility model, so all equivalent changes or modifications made according to the structure, features and principles described in the scope of the present utility model patent application are included in the scope of the present utility model patent application.
Claims
1. A PCR reaction tube capable of effectively preventing contamination, comprising a test tube (1), characterized in that: A limiting groove (7) is provided at the top of the inner wall of the test tube (1); an isolation chamber (2) is rotatably provided at the top of the test tube (1); a clamping ring (8) is fixedly installed on the side wall of the bottom end of the isolation chamber (2); the clamping ring (8) is arranged inside the limiting groove (7); a sealing plate (15) is fixedly installed at the top and bottom of the isolation chamber (2); a sealing cover (3) is rotatably connected to the top of the sealing plate (15) near the top of the isolation chamber (2); and the top and bottom of the isolation chamber (2) are fixedly installed with a sealing plate (15). A card slot (4) is provided on each side of the sealing cover (3); card blocks (5) are fixedly installed on both sides of the bottom of the sealing cover (3) and both sides of the top of the test tube (1); the card blocks (5) are adapted to the card slot (4); a first sampling port (6) is provided on one side of the top of the sealing cover (3); a connecting port (13) is provided on the side of the two sealing plates (15) away from the first sampling port (6); and a second sampling port (14) is provided on the side of the top of the test tube (1) away from the connecting port (13).
2. A PCR reaction tube capable of effectively preventing contamination according to claim 1, characterized in that: The side walls of the isolation chamber (2) and the test tube (1) are both provided with anti-slip grooves (11).
3. A PCR reaction tube capable of effectively preventing contamination according to claim 2, characterized in that: Paddles (9) are fixedly mounted on both sides of the top of the sealing cover (3).
4. The PCR reaction tube capable of effectively preventing contamination according to claim 2, characterized in that: The top of the sealing cover (3) is provided with a marking groove (10).
5. The PCR reaction tube capable of effectively preventing contamination according to claim 2, characterized in that: Silicone sheets (12) are fixedly mounted on the bottom of the sealing cover (3), the top of the test tube (1) and the two sealing plates (15), and the plurality of silicone sheets (12) are in contact with the first sampling port (6), the second sampling port (14) and the two connecting ports (13) respectively.
6. The PCR reaction tube capable of effectively preventing contamination according to claim 2, characterized in that: The test tube (1), the isolation chamber (2) and the sealing cover (3) are all made of transparent plastic.