A cervical cancer gene methylation detection device

Abstract

The utility model discloses a cervical cancer gene methylation detection device, including a detection box and a base, the detection box is detachably mounted on the base, a temperature control chamber is arranged inside the detection box, the interior of the temperature control chamber is filled with a heating medium, a plurality of placement tubes are evenly distributed inside the detection box, the lower end of each placement tube is inserted into the temperature control chamber and immersed in the heating medium in the temperature control chamber, the utility model forms a circulating flow system with the heating medium in the temperature control chamber through the operation of a micro liquid pump, so that the heating medium in the liquid box can be heated or cooled by controlling the heating component to maintain a stable and balanced temperature, thereby reducing operating errors, improving the accuracy and reliability of the experiment, and ensuring the accuracy and stability of gene methylation detection is crucial, which helps to improve the early diagnosis and treatment of cervical cancer.

Description

Technical Field

[0001] The utility model relates to a cervical cancer gene methylation detection device, belonging to the technical field of medical detection. Background Art

[0002] The cervical cancer gene methylation detection process includes several key steps, starting from sample lysis, through nucleic acid sulfite conversion, nucleic acid purification, and finally reaching fluorescent PCR detection. Each step is crucial because they ensure that the methylation status can be accurately extracted and detected from the sample.

[0003] However, existing cervical cancer gene methylation detection devices face challenges in maintaining a stable experimental temperature. Stable temperature is a key factor in ensuring experimental consistency and accuracy. Since ordinary equipment cannot provide a stable and balanced temperature environment, this leads to significant differences in the test results of the same batch of samples at different time periods, which affects the accuracy of the results and may even lead to experimental failure.

[0004] Therefore, a cervical cancer gene methylation detection device is proposed. Utility Model Content

[0005] The purpose of the utility model is to provide a cervical cancer gene methylation detection device to solve the problems raised in the above background technology.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a cervical cancer gene methylation detection device, comprising a detection box and a base, the detection box being detachably mounted on the base;

[0007] A temperature control chamber is arranged inside the test box, and the interior of the temperature control chamber is filled with a heating medium. A plurality of placement cylinders for placing test tubes are evenly distributed inside the test box, and the lower end of each placement cylinder is inserted into the temperature control chamber and immersed in the heating medium in the temperature control chamber;

[0008] A liquid box for containing a heating medium is fixedly connected to the interior of the base, a heating component for heating the heating medium in the liquid box is arranged inside the base, a micro liquid pump is arranged inside the base, a liquid suction tube of the micro liquid pump is plugged into the interior of the liquid box, an end of the liquid outlet pipe of the micro liquid pump extends upward and is connected to the temperature control chamber, a liquid return pipe is connected to the temperature control chamber, and a lower end of the liquid return pipe extends to the interior of the liquid box, and when the micro liquid pump is running, the heating medium circulates in the temperature control chamber and the liquid box.

[0009] Preferably, a plurality of partitions are evenly distributed inside the temperature control chamber, which enable the heating medium to flow in a serpentine manner in the temperature control chamber.

[0010] Preferably, the heating assembly includes a heating tube and a heating module. The heating tube is arranged in the base and located at the bottom of the liquid storage box. The heating module is fixed in the base and electrically connected to the heating tube. When the heating tube is heated, the heat of the heating tube is radiated to the bottom of the liquid storage box to adjust the temperature of the heating medium.

[0011] Preferably, the heating tube is distributed in a multi-section U-shaped shape at the bottom of the liquid storage box.

[0012] Preferably, the liquid storage box is made of aluminum alloy to improve thermal conductivity and increase heating efficiency of the heating medium.

[0013] Preferably, a manipulator is provided on the outer wall of the base, and a display screen and buttons are provided on the manipulator. The manipulator is electrically connected to the micro liquid pump and the heating module. The operation of the micro liquid pump and the heating module is controlled by the buttons provided on the manipulator. A power interface is also provided on one side of the base.

[0014] Preferably, a box cover capable of sealing the upper end of the test box is provided above the test box, and closing the box cover can prevent heat loss, thereby improving the heat preservation effect.

[0015] Preferably, fixed blocks are fixedly installed on both sides of the detection box, and a card block is slidably connected to the inside of the fixed block through a spring. The spring exerts a spring-pressing effect on the card block, causing the end of the card block to pass through the fixed block and extend outside the fixed block. Card slots are provided on both sides of the base. When the detection box is installed on the base, the card block is connected to the inside of the card slot, thereby realizing stable installation of the detection box on the base.

[0016] Compared with existing technologies:

[0017] 1. The utility model forms a circulating flow system between the liquid box and the heating medium in the temperature control chamber through the operation of the micro liquid pump. In this way, the heating medium in the liquid box can be heated or cooled by controlling the heating component to maintain a stable and balanced temperature, thereby reducing operating errors, improving the accuracy and reliability of the experiment, and ensuring the accuracy and stability of gene methylation detection is crucial, which helps to improve the early diagnosis and treatment of cervical cancer.

[0018] 2. The utility model presses the card block to cause the card block to compress the spring and retract into the fixed block. At this time, the detection box is installed on the base. When the card block is aligned with the card slot, the spring presses the card block, and the card block is connected to the inside of the card slot. Under the connection between the card block and the card slot, the detection box can be stably installed on the base. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2This is a schematic diagram of the box cover of the utility model being opened;

[0021] Figure 3 It is an exploded view of the detection box and the base of the utility model;

[0022] Figure 4 It is a cross-sectional view of the overall structure of the utility model;

[0023] Figure 5 It is a cross-sectional view of the detection box, temperature control chamber and placement tube of the utility model;

[0024] Figure 6 For the utility model Figure 5 Enlarged view of point A.

[0025] In the figure: 1. detection box, 101. temperature control chamber, 2. base, 3. placement tube, 4. liquid storage box, 5. micro liquid pump, 501. suction tube, 502. liquid outlet pipe, 6. liquid return pipe, 7. heating component, 701. heating tube, 702. heating module, 8. partition, 9. manipulator, 10. power interface, 11. box cover, 12. fixing block, 13. spring, 14. card block, 15. card slot. DETAILED DESCRIPTION

[0026] The present invention is described below with specific embodiments, but is not intended to be a limitation of the present invention.

[0027] Example 1

[0028] like Figure 1-Figure 5 As shown, in this embodiment, a cervical cancer gene methylation detection device is provided, comprising a detection box 1 and a base 2, and the detection box 1 is detachably mounted on the base 2;

[0029] A temperature control chamber 101 is provided inside the detection box 1, and the interior of the temperature control chamber 101 is filled with a heating medium. A plurality of placement cylinders 3 for placing test tubes are evenly distributed inside the detection box 1, and the lower end of each placement cylinder 3 is inserted into the temperature control chamber 101 and immersed in the heating medium in the temperature control chamber 101;

[0030] A liquid box 4 for containing a heating medium is fixedly connected to the interior of the base 2, a heating assembly 7 for heating the heating medium in the liquid box 4 is arranged inside the base 2, a micro liquid pump 5 is arranged inside the base 2, a liquid pipette 501 of the micro liquid pump 5 is plugged into the interior of the liquid box 4, an end of a liquid outlet pipe 502 of the micro liquid pump 5 extends upward and communicates with the temperature control chamber 101, a liquid return pipe 6 is connected to the temperature control chamber 101, and a lower end of the liquid return pipe 6 extends to the interior of the liquid box 4. When the micro liquid pump 5 is running, the heating medium circulates in the temperature control chamber 101 and the liquid box 4;

[0031] When the micro liquid pump 5 is running, it will suck the heating medium in the liquid box 4 through the liquid pipette 501, and then pump it into the temperature control chamber 101 through the liquid outlet pipe 502. In the temperature control chamber 101, the heating medium will perform heat exchange around the placement tube 3 immersed therein, thereby regulating the temperature in the placement tube 3. At this time, the test tube is placed inside the placement tube 3, and the temperature control of the sample in the test tube is then ensured.

[0032] The heating medium after heat exchange flows back into the liquid box 4 through the return pipe 6 to form a circulating flow system. In this way, the heating medium in the liquid box 4 can be heated or cooled by controlling the heating component 7, thereby indirectly controlling the temperature of the sample in the test tube to meet the experimental requirements.

[0033] like Figure 4 , Figure 5 As shown, in order to make the heating medium in the temperature control chamber 101 flow smoothly and make the temperature of the heating medium in the temperature control chamber 101 uniform, a plurality of partitions 8 are evenly distributed inside the temperature control chamber 101 to enable the heating medium to flow in a serpentine manner in the temperature control chamber 101. The partitions 8 divide the temperature control chamber 101 into different small chambers, and the upper ends or lower ends of adjacent small chambers are connected (such as Figure 5 As shown, for two adjacent partitions 8, a through hole is opened at the upper end of one of the partitions 8, and a through hole is opened at the lower end of the other partition 8.) After the liquid outlet pipe 502 guides the heating medium into the temperature control chamber 101, the heating medium flows in the small cavity in turn, and finally, flows back to the liquid storage box 4 from the return liquid pipe 6.

[0034] like Figure 4 As shown, in order to heat the heating medium in the liquid box 4, the heating assembly 7 includes a heating tube 701 and a heating module 702. The heating tube 701 is arranged in the base 2 and is located at the bottom of the liquid box 4. The heating module 702 is fixed in the base 2 and is electrically connected to the heating tube 701. When the heating tube 701 is heated, the heat of the heating tube 701 is radiated to the bottom of the liquid box 4, thereby adjusting the temperature of the heating medium.

[0035] The heating tube 701 is distributed at the bottom of the liquid storage box 4 in a multi-section U-shaped shape.

[0036] The liquid storage box 4 is made of aluminum alloy to improve the thermal conductivity and increase the heating efficiency of the heating medium.

[0037] A manipulator 9 is arranged on the outer wall of the base 2, and a display screen and buttons are arranged on the manipulator 9. The manipulator 9 is electrically connected to the micro liquid pump 5 and the heating module 702. The operation of the micro liquid pump 5 and the heating module 702 can be controlled by the buttons arranged on the manipulator 9, and the display screen can display the operating status information of the micro liquid pump 5 and the heating module 702 (for example, the pump liquid volume of the micro liquid pump 5, the temperature of the heating module 702, etc.). A power interface 10 is also arranged on one side of the base 2.

[0038] A box cover 11 is provided above the detection box 1 to seal the upper end of the detection box 1 . Closing the box cover 11 can prevent heat loss, thereby improving the heat preservation effect.

[0039] Example 2

[0040] like Figure 3 , Figure 5 as well as Figure 6 As shown, on the basis of Example 1, in order to stably install the detection box 1 on the base 2, the present embodiment has fixed blocks 12 fixedly installed on both sides of the detection box 1, and the interior of the fixed block 12 is slidably connected with a clamping block 14 through a spring 13. The spring 13 plays a role of elastic pressure on the clamping block 14, so that the end of the clamping block 14 passes through the fixed block 12 and extends to the outside of the fixed block 12, and the base 2 has a clamping groove 15 on both sides. When the detection box 1 is installed on the base 2, the clamping block 14 is clamped in the interior of the clamping groove 15, so that the detection box 1 is stably installed on the base 2;

[0041] The process of installing the detection box 1 on the base 2 is as follows:

[0042] The card block 14 is pressed, so that the card block 14 compresses the spring 13 and retracts into the fixed block 12. In this state, the detection box 1 is installed on the base 2. When the card block 14 is aligned with the card slot 15, the spring 13 presses the card block 14, so that the card block 14 is engaged inside the card slot 15. In this way, the card block 14 and the card slot 15 are engaged, and then the detection box 1 can be stably installed on the base 2.

[0043] The process of removing the detection box 1 from the base 2 is as follows:

[0044] The block 14 is pressed, so that the block 14 compresses the spring 13 and retracts into the fixed block 12 . In this state, the block 14 is withdrawn from the inside of the slot 15 , and the detection box 1 is pulled upward, and the detection box 1 can be quickly removed from the base 2 .

[0045] In addition, when the test box 1 is disassembled and assembled on the base 2, in order to prevent the liquid outlet pipe 502 and the liquid return pipe 6 from interfering with the disassembly and assembly of the test box 1, the liquid outlet pipe 502 and the liquid return pipe 6 are divided into two parts, one part is arranged in the test box 1, and the other part is arranged in the base 2. When the test box 1 is installed on the base 2, the two parts are connected by a quick connector, which ensures that the test box 1 can be smoothly disassembled and assembled on the base 2, and can also ensure that the liquid outlet pipe 502 and the liquid return pipe 6 can pass smoothly.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A cervical cancer gene methylation detection device, characterized in that: It comprises a detection box (1) and a base (2), wherein the detection box (1) is detachably mounted on the base (2); The detection box (1) is provided with a temperature control chamber (101) inside, the temperature control chamber (101) is filled with a heating medium, a plurality of placement cylinders (3) for placing test tubes are evenly distributed inside the detection box (1), and the lower end of each placement cylinder (3) is inserted into the temperature control chamber (101) and immersed in the heating medium in the temperature control chamber (101); A liquid storage box (4) for storing a heating medium is fixedly connected to the interior of the base (2); a heating component (7) for heating the heating medium in the liquid storage box (4) is arranged inside the base (2); a micro liquid pump (5) is arranged inside the base (2); a liquid suction pipe (501) of the micro liquid pump (5) is plugged into the interior of the liquid storage box (4); an end of a liquid outlet pipe (502) of the micro liquid pump (5) extends upward and communicates with a temperature control chamber (101); a liquid return pipe (6) is connected to the temperature control chamber (101); a lower end of the liquid return pipe (6) extends to the interior of the liquid storage box (4); when the micro liquid pump (5) is in operation, the heating medium circulates in the temperature control chamber (101) and the liquid storage box (4).

2. A cervical cancer gene methylation detection device according to claim 1, characterized in that: A plurality of partitions (8) are evenly distributed inside the temperature control chamber (101) and are capable of causing the heating medium to flow in a serpentine manner inside the temperature control chamber (101).

3. A cervical cancer gene methylation detection device according to claim 1, characterized in that: The heating assembly (7) comprises a heating tube (701) and a heating module (702); the heating tube (701) is arranged in the base (2) and located at the bottom of the liquid storage box (4); the heating module (702) is fixed in the base (2) and electrically connected to the heating tube (701).

4. A cervical cancer gene methylation detection device according to claim 3, characterized in that: The heating tube (701) is in a multi-section U-shaped configuration and is distributed at the bottom of the liquid storage box (4).

5. A cervical cancer gene methylation detection device according to claim 3, characterized in that: The liquid storage box (4) is made of aluminum alloy.

6. A cervical cancer gene methylation detection device according to claim 3, characterized in that: A controller (9) is arranged on the outer wall of the base (2), and a display screen and buttons are arranged on the controller (9). A power interface (10) is also arranged on one side of the base (2).

7. A cervical cancer gene methylation detection device according to claim 1, characterized in that: A box cover (11) capable of closing the upper end of the detection box (1) is provided above the detection box (1).

8. A cervical cancer gene methylation detection device according to claim 1, characterized in that: Fixed blocks (12) are fixedly mounted on both sides of the detection box (1), a clamping block (14) is slidably connected to the interior of the fixed block (12) via a spring (13), and clamping grooves (15) are provided on both sides of the base (2). When the detection box (1) is mounted on the base (2), the clamping block (14) is clamped in the interior of the clamping groove (15).

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