Single-hole PCR temperature control component and single-hole PCR detector

Abstract

The present application discloses a single-hole PCR temperature control component and a single-hole PCR detector, including: a PCR reactor, a plurality of heat dissipation fins are fixedly arranged along the circumference of the outer wall of the PCR reactor and a wind channel is formed between adjacent heat dissipation fins; a heating film is arranged at the bottom of the PCR reactor; a fan is also arranged on the side of the PCR reactor; and at least two optical fiber connection holes that penetrate the outer wall are opened on the outer wall of the PCR reactor. In the present application, only one PCR reactor is provided, and the detection of the reaction tube can be completed without configuring a rotatable fluorescent scanning component; and the heat sink and cooling fin structure are eliminated in the temperature control component, and the heat dissipation fins are integrated with the PCR reactor, which can ensure temperature control and detection efficiency while making the entire PCR detector small in size, easy to carry, light in weight, and can improve manufacturing efficiency and reduce manufacturing costs.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection experimental equipment, in particular to a single-hole PCR temperature control component and a single-hole PCR detector. Background Art

[0002] In the conventional PCR detection instrument used for batch detection, a plurality of modular PCR reactors are provided, wherein the PCR reactor is generally a cylindrical structure with an open top and a closed bottom, and has an inverted cone-shaped accommodation space inside, which is used to accommodate the reaction tube containing the sample to be tested. The PCR reactor module, the refrigeration plate assembly and the radiator arranged at the bottom thereof in sequence together form a temperature control assembly, which controls the temperature of the thermal cycle reaction occurring in the reactor when the PCR detection instrument is working. In the conventional PCR detector, the semiconductor refrigeration plate has the characteristics of being cold on the top and hot on the bottom, and the radiator must be arranged at the bottom of the refrigerator. Therefore, the PCR reactor, the semiconductor refrigeration plate, and the radiator must be stacked in the upper, middle and lower directions. Therefore, the volume is generally large and inconvenient to move. It can only be fixed for batch detection, and it needs to be equipped with a rotatable fluorescence scanning component to realize the fluorescence detection in multiple PCR reactors; even if only one PCR reactor is set, due to the existence of the refrigeration plate and the radiator structure, it is impossible to realize the needs of individual users for portability, lightness, small size, and on-the-go detection. Utility Model Content

[0003] In view of the above-mentioned shortcomings or deficiencies of the prior art, the technical problem to be solved by the present invention is to provide a single-hole PCR temperature control component and a method thereof.

[0004] To solve the above technical problems, this application is implemented through the following technical solutions:

[0005] A single-hole PCR temperature control component comprises: a PCR reactor, a heat dissipation fin, and a heating film. The heat dissipation fin is fixed to the outer side wall of the PCR reactor to form an air channel, and the heating film is arranged at the bottom of the PCR reactor.

[0006] Furthermore, the inner wall of the PCR reactor is in an inverted cone shape, and the heat dissipation fins are circumferentially arranged around the outer wall of the PCR reactor.

[0007] Furthermore, the wind channel formed by the heat dissipation fins is continuous in the circumferential direction.

[0008] Furthermore, the heat dissipation fins are arranged in plurality, and the plurality of heat dissipation fins are arranged parallel to each other and / or coaxially and centrally symmetrically.

[0009] Furthermore, the PCR reactor and the plurality of heat dissipating fins have the same central axis.

[0010] Furthermore, the PCR reactor is integrally arranged with the plurality of heat dissipation fins.

[0011] The present application also proposes a single-hole PCR detector, comprising: a base, a shell, a heat cover, at least one excitation light component, at least one detection component, a control main board, a fan and the single-hole PCR temperature control component as described above, wherein the base matches the shell and is fixedly connected, and the single-hole PCR temperature control component, the fan, the excitation light component, the detection component and the control main board are all fixed on the base; the excitation light component is connected to the PCR reactor through an excitation light optical fiber and is electrically connected to the control main board, and the detection component is connected to the PCR reactor through a detection light optical fiber and is electrically connected to the control main board; the heat cover is arranged on the top of the shell, matches the shell and can be opened and closed; the fan is arranged facing the side of the PCR reactor, and the fan outlet air flows through the wind channel formed by the heat dissipation fins.

[0012] Furthermore, the single-hole PCR detector also includes: a control motor and a flipping mechanism, wherein the control motor is disposed in the housing, the control motor is rotationally connected to the flipping mechanism, and the flipping mechanism is fixedly connected to the heat cover and drives the heat cover to rotate.

[0013] Furthermore, the excitation light assembly includes: a first shell, a light exit hole arranged on the first shell, an LED light source, a first lens, a first filter, a second lens and a third lens arranged in sequence along the light exit direction of the light exit hole; the LED light source is fixed on the control main board.

[0014] Furthermore, the detection component includes: a second shell, a light entrance hole arranged on the second shell, a fourth lens, a second filter, a fifth lens and a photoelectric conversion element arranged in sequence along the light entrance direction of the light entrance hole; the photoelectric conversion element is fixed on the control main board.

[0015] Compared with the prior art, this application has the following technical effects:

[0016] (1) In the present application, only one PCR reactor is provided, and PCR detection can be completed without configuring a rotatable fluorescent scanning component; and the heat sink and cooling fin structure are eliminated in the temperature control component, and the heat sink fins are integrated with the PCR reactor. While ensuring temperature control and detection efficiency, the entire PCR detector can be small in size, easy to carry, and light in weight, and can also improve manufacturing efficiency and reduce manufacturing costs.

[0017] (2) In the present application, heat dissipation fins are arranged on the outer wall of the reactor to form an air channel. When the PCR thermal cycle reaction is carried out, the heating film can heat the PCR reactor. The air channel is conducive to the air flow blown out by the fan to quickly dissipate the heat of the PCR reactor through the air channel, thereby achieving temperature control. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Other features, objects and advantages of the present application will become more apparent by reading the detailed description of non-limiting embodiments made with reference to the following drawings:

[0019] Figure 1 : A schematic diagram of the structure of a single-hole PCR temperature control assembly according to an embodiment of the present application;

[0020] Figure 2 : A cross-sectional view of the local structure of a single-hole PCR temperature control component according to an embodiment of the present application;

[0021] Figure 3 : A schematic diagram of the structure of a single-hole PCR detector according to an embodiment of the present application;

[0022] Figure 4 : A cross-sectional view of a single-hole PCR detector according to an embodiment of the present application;

[0023] Figure 5 : A schematic diagram of the partial structure of a single-hole PCR detector according to an embodiment of the present application;

[0024] Figure 6 : A top view of a single-hole PCR detector according to an embodiment of the present application;

[0025] Figure 7 : A cross-sectional view of a single-hole PCR detector in working state according to an embodiment of the present application;

[0026] Figure 8 : A cross-sectional view of an excitation light component and a detection component of a single-hole PCR detector according to an embodiment of the present application

[0027] Fig. 9 : A schematic diagram of the partial structure of a single-hole PCR temperature control component according to another embodiment of the present application;

[0028] Fig.10 : A schematic diagram of the partial structure of a single-hole PCR temperature control component according to another embodiment of the present application;

[0029] Fig.11 : A schematic diagram of the partial structure of a single-hole PCR temperature control component according to another embodiment of the present application;

[0030] Fig.12 : Schematic diagram of the partial structure of a single-hole PCR temperature control component according to another embodiment of the present application.

[0031] In the figure: 1-PCR reactor, 2-heating fins, 21-wind channel, 3-heating film, 4-blower, 5-optical fiber connection hole, 6-base, 7-housing, 73-control mainboard, 8-excitation light component, 81-LED light source, 82-first lens, 83-first filter, 84-second lens, 85-third lens, 86-light output hole, 87-excitation light fiber, 9-detection component, 91-photoelectric conversion element, 92-fifth lens, 93-second filter, 94-fourth lens, 95-light input hole, 96-detection light fiber, 10-heat cover, 11-control motor, 12-reaction tube, 13-flipping mechanism, 131-rotation axis. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0033] If there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0034] like Figure 1 and Figure 2As shown, in this embodiment, a single-hole PCR temperature control component includes: a PCR reactor 1, a plurality of heat dissipation fins 2 arranged in parallel, coaxially and in a centrally symmetrical state are fixedly arranged along the outer wall of the PCR reactor 1, and an air channel 21 is formed between adjacent heat dissipation fins 2; the inner wall of the PCR reactor is in an inverted cone shape, the heat dissipation fins 2 are arranged circumferentially around the PCR reactor, and the air channel is continuous circumferentially. A heating film 3 is closely arranged at the bottom of the PCR reactor 1; at least two optical fiber connection holes 5 that penetrate the outer wall are opened on the outer wall of the PCR reactor 1. Through the above arrangement, in this example, only one PCR reactor is provided, and the detection of the reaction tube can be completed without configuring a rotatable fluorescent scanning component; and the heat sink and cooling fin structure are eliminated in the temperature control component, and the heat dissipation fins are integrated with the PCR reactor, which can ensure the temperature control and detection efficiency while making the entire temperature control component small in size, easy to carry, light in weight, and can improve the manufacturing efficiency and reduce the manufacturing cost.

[0035] Preferably, in this embodiment, the plurality of heat dissipation fins 2 are arranged parallel, coaxially, and symmetrically, the inner wall of the PCR reactor 1 is inverted conical, and the plurality of heat dissipation fins 2 are arranged circumferentially around the PCR reactor 1, and the wind channel 21 is continuous circumferentially. The above arrangement is more conducive to ensuring the stability of the wind speed in the wind channel while reducing the volume of the single-hole PCR temperature control component, making it easier to install and carry. In other embodiments, such as Figure 9-12 As shown, it is only necessary to form an air channel, and the heat dissipation fins may not be multiple, parallel, coaxial or symmetrical.

[0036] Furthermore, in this embodiment, the PCR reactor 1 is integrally provided with the plurality of heat dissipation fins 2. The above arrangement is conducive to improving the production efficiency, thereby reducing the production cost.

[0037] Furthermore, in this embodiment, the optical fiber connection hole 5 is opened at a position of the outer wall where the heat dissipation fins 2 are not provided.

[0038] like Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7As shown, in this embodiment, a single-hole PCR detector is also proposed, including: a base 6, a shell 7, two excitation light components 8, two detection components 9, a heat cover 10, a control main board 73, a fan 4 and the single-hole PCR temperature control component as described above, the base 6 matches and is fixedly connected to the shell 7, the single-hole PCR temperature control component, the fan 4, the excitation light component 8 and the detection component 9 are all fixed on the base 6, the excitation light component 8 is electrically connected to the control main board 73, the detection component 9 is electrically connected to the control main board 73, the excitation light component 8 is connected to the excitation light optical fiber 87 and the excitation light optical fiber 87 passes through the optical fiber connection hole 5, the detection component 9 is connected to the detection light optical fiber 96 and the detection light optical fiber 96 passes through the optical fiber connection hole 5. The heat cover 10 is arranged on the top of the shell 7, matches and is open and closed with the shell 7. The fan 4 is arranged on the side of the PCR reactor 1, facing the PCR reactor, and the fan outlet air flows through the wind channel 21 formed by the heat dissipation fins 2 to dissipate heat from the PCR reactor.

[0039] Furthermore, in this embodiment, the single-hole PCR detector also includes: a control motor 11, which is arranged in the shell 7, and the control motor 11 is rotatably connected to the flip mechanism 13, and the rotating shaft 131 of the flip mechanism 13 passes through one end of the heat cover 10 and is fixedly connected to the heat cover 10 to drive the heat cover 10 to open and close automatically.

[0040] Alternatively, those skilled in the art can easily know that the thermal cover 10 can be opened and closed manually, or can be opened and closed automatically by other methods such as horizontal pulling.

[0041] Specifically, when the PCR detector is working, the reaction tube 12 containing the sample to be tested is placed in the PCR reactor, the thermal cover is closed, the interior of the shell is sealed, and the thermal cover is in direct contact with the tube cover of the reaction tube, which can prevent the steam generated during the PCR reaction from condensing and forming water droplets, thereby reducing the reaction system.

[0042] like Figure 8 As shown, in this embodiment, the excitation light component 8 includes: a first shell, a light outlet hole 86 arranged on the first shell, an LED light source 81, a first lens 82, a first filter 83, a second lens 84 and a third lens 85 arranged in sequence along the light outlet direction of the light outlet hole 86; the LED light source 81 is fixed on the control main board 73.

[0043] Furthermore, in this embodiment, the detection component 9 includes: a second shell, a light entrance hole 95 arranged on the second shell, a fourth lens 94, a second filter 93, a fifth lens 92 and a photoelectric conversion element 91 arranged in sequence along the light entrance direction of the light entrance hole 95; the photoelectric conversion element 91 is fixed on the control main board 73.

[0044] When starting work, the LED light source 81 of the excitation light component 8 emits excitation light, which enters the PCR reactor 1 along the excitation light optical fiber 87. PCR reaction is carried out in the PCR reactor 1 and fluorescence is generated. The light enters the detection component 9 from the detection optical fiber 96 and finally reaches the photoelectric conversion element 91. The PCR detection result is obtained based on the detection of fluorescence.

[0045] When the PCR reaction is performed, heating is performed by the heating film 3 closely arranged at the bottom of the PCR reactor 1, and heat is dissipated by the heat dissipation fins 2 and the fan 4, so that different temperature requirements of the PCR reaction can be met.

[0046] Compared with the prior art, this application has the following technical effects:

[0047] In the present application, only one PCR reactor is provided, and detection can be completed without configuring a rotatable fluorescent scanning component; and the radiator and cooling fin structure are eliminated in the temperature control component, and the heat dissipation fins are integrated with the PCR reactor. While ensuring temperature control and detection efficiency, the entire PCR detector can be small in size, easy to carry, and light in weight, and can also improve manufacturing efficiency and reduce manufacturing costs.

[0048] In the present application, heat dissipation fins are arranged on the outer wall of the reactor to form an air channel. When the PCR thermal cycle reaction is carried out, the heating film can heat the PCR reactor. The air channel is conducive to the air flow blown out by the fan to quickly dissipate the heat of the PCR reactor through the air channel, thereby achieving temperature control.

[0049] The above embodiments are only used to illustrate the technical solution of the present application but not to limit it. The present application is described in detail with reference to the preferred embodiments. It should be understood by those skilled in the art that the technical solution of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present application, and should be included in the scope of the claims of the present application.

Claims

1. A single-hole PCR temperature control component, characterized in that: include: A PCR reactor, a heat dissipation fin, and a heating film. The heat dissipation fin is fixed to the outer side wall of the PCR reactor to form an air channel, and a heating film is arranged at the bottom of the PCR reactor.

2. The single-hole PCR temperature control assembly according to claim 1, characterized in that: The inner wall of the PCR reactor is in an inverted cone shape, and the heat dissipation fins are circumferentially arranged around the outer wall of the PCR reactor.

3. The single-hole PCR temperature control assembly according to claim 2, characterized in that: The wind channel formed by the heat dissipation fins is continuous in the circumferential direction.

4. The single-hole PCR temperature control assembly according to claim 1, characterized in that: The heat dissipation fins are arranged in plurality, and the plurality of heat dissipation fins are arranged parallel to each other and / or coaxially and centrally symmetrically.

5. The single-hole PCR temperature control assembly according to claim 4, characterized in that: The PCR reactor and the plurality of heat dissipating fins have the same central axis.

6. The single-hole PCR temperature control assembly according to any one of claims 1 to 5, characterized in that: The PCR reactor is integrally arranged with the plurality of heat dissipation fins.

7. A single-hole PCR detector, characterized in that: include: A base, a shell, a heat cover, at least one excitation light component, at least one detection component, a control mainboard, a fan and a single-hole PCR temperature control component as described in any one of claims 1 to 6, wherein the base matches the shell and is fixedly connected, and the single-hole PCR temperature control component, the fan, the excitation light component, the detection component and the control mainboard are all fixed on the base; the excitation light component is connected to the PCR reactor through an excitation light optical fiber and is electrically connected to the control mainboard, and the detection component is connected to the PCR reactor through a detection light optical fiber and is electrically connected to the control mainboard; the heat cover is arranged on the top of the shell, matches the shell and can be opened and closed, and the fan is arranged facing the side of the PCR reactor, and the fan outlet air flows through the wind channel formed by the heat dissipation fins.

8. The single-hole PCR detector according to claim 7, characterized in that: Also includes: A control motor and a flipping mechanism, wherein the control motor is arranged in the housing, the control motor is rotationally connected to the flipping mechanism, and the flipping mechanism is fixedly connected to the heat cover and drives the heat cover to rotate.

9. The single-hole PCR detector according to claim 7, characterized in that: The excitation light assembly includes: a first shell, a light outlet hole arranged on the first shell, an LED light source, a first lens, a first filter, a second lens and a third lens arranged in sequence along the light outlet direction of the light outlet hole; the LED light source is fixed on the control mainboard.

10. The single-hole PCR detector according to claim 7, characterized in that: The detection component includes: a second shell, a light entrance hole arranged on the second shell, a fourth lens, a second filter, a fifth lens and a photoelectric conversion element arranged in sequence along the light entrance direction of the light entrance hole; the photoelectric conversion element is fixed on the control mainboard.

Images