A tablet pressing device for staining early embryos at various stages

Abstract

The utility model provides a slide pressing device that is beneficial to the staining of early embryos at various stages, including an upper plate and a lower plate, the side ends of the upper plate and the lower plate are hinged by a movable hinge, a cover glass groove is provided at the bottom end of the upper plate, a silicone gasket is fixedly installed in the cover glass groove, and a slide glass groove is provided at the top end of the lower plate, a silicone gasket is fixedly installed in the slide glass groove, and the cover glass groove corresponds to the slide glass groove. The utility model has a cover glass groove on the upper plate and a slide glass groove on the lower plate, and the cover glass and the slide glass are limited by the cover glass groove and the slide glass groove to prevent the cover glass and the slide glass from shifting when they are pressed, and the cover glass can be pressed against the slide glass by pressing the upper plate, and the force is dispersed by the upper plate, so that the force is uniform when pressing the slide, and no fingerprints will be left on the pressed slide, and the sight line observed under the stereoscope will not be blocked when pressing the slide, which is convenient for observing while pressing the slide, and adjusting the pressing force, which greatly improves the success rate of pressing the slide.

Description

Technical Field

[0001] The utility model relates to an embryo tablet pressing device, in particular to a tablet pressing device which is beneficial to dyeing early embryos at various stages. Background Art

[0002] In the immunofluorescence experiment of early animal embryos, the early embryos of each developmental stage are gradually stained and then need to be pressed for fluorescence imaging. The purpose is to press the three-dimensional spherical embryo into a round cake shape to meet the requirements of microscopic imaging as much as possible (ordinary upright or inverted microscopes can only perform planar imaging; although laser confocal can perform slice scanning imaging, it is also limited to a certain scanning range and requires the superposition of multiple layers of imaging). Simply put, pressing is to place the embryo on a glass slide, cover it with a coverslip and press it as thin as possible with a complete shape.

[0003] There are no unified steps and standards for the compression operation, and there are also matters that need to be paid attention to when compressing embryos at different stages. For example: the degree of compression of embryos at the same developmental stage must be uniform, otherwise the embryos will be of different sizes when imaged; the force must be even during the compression process, otherwise the embryos will easily be crushed, causing the blastomere to flow out of the ruptured zona pellucida and destroying the integrity of the embryo; uneven compression force will cause different thicknesses of the compression, making it difficult to focus on a visual plane during microscopic observation.

[0004] Currently, many laboratories use viscous substances such as lanolin and paraffin when staining and pressing slides. They apply them to the four corners of the coverslip to buffer the pressure of the coverslip. However, when placing the coverslip on the slide and slowly pressing around, it is easy to cause the coverslip to shift, resulting in the crushing of the pressed embryo and failure of pressing. To improve the success rate of pressing, a certain amount of practical operation experience and foundation is required, which is bound to consume a lot of time and experimental repetition costs.

[0005] Therefore, making the tableting operation simple and efficient without overly relying on the operator's technical level is a key issue that needs to be urgently addressed to improve the efficiency of embryo tableting. Utility Model Content

[0006] The technical problem to be solved by the utility model is to provide a tablet pressing device which is beneficial to the staining of early embryos at various stages in view of the deficiencies of the above-mentioned prior art. The tablet pressing device has a simple structure, is easy to operate, has a good use effect, and can be popularized and applied.

[0007] In order to solve the above technical problems, the technical solution adopted by the utility model is: a slide pressing device which is beneficial to the staining of early embryos at various stages, comprising an upper plate and a lower plate, the upper plate and the lower plate having the same specifications and dimensions, the upper plate being 100 mm long, 45 mm wide, and 4 mm thick, the side ends of the upper plate and the lower plate being hinged by a movable hinge, and characterized in that two cover glass grooves are provided at the bottom end of the upper plate, the cover glass grooves being 21 mm long, 21 mm wide, and 0.53 to 0.56 mm deep.

[0008] Silicone gaskets are fixedly installed at the four end corners in the cover glass groove, and the silicone gaskets are 5 mm long, 5 mm wide and 0.4 mm thick.

[0009] A glass slide groove is provided at the top of the lower plate, which is 75 mm long, 25 mm wide and 1.0-1.2 mm deep. Silicone gaskets are also fixedly installed at the four end corners of the glass slide groove, which are 5 mm long, 5 mm wide and 0.4 mm thick. The two cover glass grooves correspond to the glass slide grooves.

[0010] Preferably, both sides of one end of the slide groove are provided with slide taking grooves to facilitate taking out the prepared pressed slides.

[0011] Preferably, the upper plate is provided with two mounting grooves, the mounting grooves are connected to the cover glass grooves respectively, a push plate is slid in the mounting grooves, and a push rod is fixedly installed on the top of the push plate. When the pressed sheet is made, the push plate is pushed by the push rod to separate the pressed sheet from the upper plate, and the pressed sheet remains in the slide glass groove for easy removal.

[0012] Compared with the prior art, the utility model has the following advantages:

[0013] The utility model is provided with a cover glass groove on the upper plate, and a slide glass groove on the lower plate, and the cover glass and the slide glass are limited by the cover glass groove and the slide glass groove to prevent the cover glass and the slide glass from shifting when they are pressed, and the cover glass can be pressed against the slide glass by pressing the upper plate, and the force is dispersed by the upper plate, so that the force is evenly applied when pressing the tablet, and fingerprints will not be left on the pressed tablet, and the sight line observed under the stereoscope will not be blocked when pressing the tablet, so that it is convenient to observe while pressing the tablet and adjust the pressing force, thereby greatly improving the success rate of tablet pressing.

[0014] The utility model is further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural schematic diagram of the utility model.

[0016] Figure 2 It is a structural schematic diagram of the upper plate in the utility model.

[0017] Figure 3It is a side view of the upper plate in the utility model.

[0018] Figure 4 It is a structural schematic diagram of the lower plate in the utility model.

[0019] Description of reference numerals:

[0020] 1—upper plate; 2—lower plate; 3—cover slip tank;

[0021] 4—Silicone gasket; 5—Glass slide slot; 6—Slide retrieval slot;

[0022] 7—Push plate. DETAILED DESCRIPTION

[0023] In order to make the above-mentioned purposes, features and advantages of the utility model more obvious and easy to understand, the specific implementation of the utility model is described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the utility model. However, the utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the utility model, so the utility model is not limited by the specific implementation disclosed below.

[0024] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0025] like Figures 1 to 3 As shown, the utility model provides a tablet pressing device which is beneficial to the staining of early embryos at various stages, comprising an upper plate 1 and a lower plate 2, wherein the upper plate 1 and the lower plate 2 have the same specifications and dimensions, and the upper plate 1 is 100 mm long, 45 mm wide and 4 mm thick.

[0026] The side ends of the upper plate 1 and the lower plate 2 are hinged by hinges, and two cover glass grooves 3 are provided at the bottom end of the upper plate 1. The cover glass grooves 3 are 21 mm long, 21 mm wide, and 0.53-0.56 mm deep, and are suitable for cover glasses with a specification of 20×20 mm and a thickness of 0.13-0.16 mm. The upper plate 1 suitable for cover glasses of different specifications can be designed according to experimental requirements.

[0027] Silicone gaskets 4 are fixedly installed at the four end corners of the cover glass groove 3. The silicone gasket 4 is 5 mm long, 5 mm wide and 0.4 mm thick.

[0028] A slide glass groove 5 is provided at the top of the lower plate 2, and the slide glass groove 5 is 75 mm long, 25 mm wide, and 1.0-1.2 mm deep. Silicone gaskets 4 are also fixedly installed at the four end corners of the slide glass groove 5, and the silicone gasket 4 is 5 mm long, 5 mm wide, and 0.4 mm thick. The two cover glass grooves 3 correspond to the slide glass groove 5.

[0029] In this embodiment, the slide groove 5 is provided with slide taking grooves 6 on both sides of one end to facilitate taking out the prepared pressed slides.

[0030] In this embodiment, two mounting grooves are provided on the upper plate 1, the mounting grooves are respectively connected to the cover glass grooves 3, a push plate 7 is slid in the mounting grooves, and a push rod is fixedly installed on the top of the push plate 7. When the pressed sheet is made, the push plate 7 is pushed by the push rod to separate the pressed sheet from the upper plate 1, and the pressed sheet remains in the slide glass groove 5 for easy removal.

[0031] When in use, the device is placed under a microscope, and the upper plate 1 and the lower plate 2 are opened.

[0032] A slide glass is placed in the slide glass slot 5, and the prepared oocytes and embryos are fixed on the slide glass.

[0033] Apply lanolin to the four corners of the cover glass, and then place the cover glass into the cover glass groove 3.

[0034] The upper plate 1 is turned over so that the upper plate 1 and the lower plate 2 are stacked together.

[0035] The cover glass falls on the slide and presses the upper plate 1 so that the cover glass squeezes the oocytes and embryos.

[0036] Observe under a microscope until the oocyte and embryo fixative are completely spread out.

[0037] The push plate 7 is pushed by the push rod to separate the pressed sheet from the upper plate 1 , and the pressed sheet remains in the slide groove 5 .

[0038] Open the upper plate 1 and take out the prepared pressed tablet from the tablet taking slot 6 .

[0039] The above is only a preferred embodiment of the utility model, and does not limit the utility model in any way. Any simple modification, change and equivalent change made to the above embodiment according to the essence of the utility model technology still falls within the protection scope of the technical solution of the utility model.

Claims

1. A tablet pressing device for staining early embryos at various stages, comprising an upper plate (1) and a lower plate (2), wherein the upper plate (1) and the lower plate (2) are hinged at their side ends by hinges, characterized in that: The upper plate (1) is provided with two cover glass grooves (3) at the bottom end, and silicone gaskets (4) are fixedly installed at the four end corners of the cover glass grooves (3). The lower plate (2) is provided with a slide glass groove (5) at the top end, and silicone gaskets (4) are also fixedly installed at the four end corners of the slide glass groove (5). The two cover glass grooves (3) correspond to the slide glass groove (5).

2. A tablet pressing device for staining early embryos at various stages according to claim 1, characterized in that: The upper plate (1) and the lower plate (2) have the same specifications and dimensions. The upper plate (1) is 100 mm long, 45 mm wide and 4 mm thick.

3. A tablet pressing device for staining early embryos at various stages according to claim 2, characterized in that: The cover glass groove (3) is 21 mm long, 21 mm wide and 0.53-0.56 mm deep.

4. A tablet pressing device for staining early embryos at various stages according to claim 3, characterized in that: The slide glass groove (5) is 75 mm long, 25 mm wide and 1.0-1.2 mm deep.

5. A tablet pressing device for staining early embryos at various stages according to claim 4, characterized in that: The silica gel gasket (4) is 5 mm long, 5 mm wide and 0.4 mm thick.

6. A tablet pressing device for staining early embryos at various stages according to claim 4, characterized in that: Slide taking grooves (6) are provided on both sides of one end of the slide groove (5).

7. A tablet pressing device for staining early embryos at various stages according to claim 1, characterized in that: The upper plate (1) is provided with two installation grooves, the installation grooves are respectively connected to the cover glass grooves (3), a push plate (7) is slidable in the installation grooves, and a push rod is fixedly mounted on the top of the push plate (7).

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