Underwater microparticle holographic microscopy device

Abstract

The present application provides an underwater micro-particle holographic microscopic imaging device, comprising an upper protective shell, the bottom of the upper protective shell is fixedly connected to a lower protective shell, a cold light CCD camera is fixedly installed inside the upper protective shell, a laser is installed inside the lower protective shell, a micron-level pinhole is arranged at the top of the laser, a measurement area is arranged between the laser and the cold light CCD camera, and a timing synchronizer is fixedly installed at the top of the upper protective shell. The utility model integrates the protective shell, the imaging adjustment component, the micron-level pinhole, the cold light CCD camera, the laser, and the timing synchronizer into one, which is highly integrated, reduces the volume, is easy to carry, and is more suitable for field working environments.

Description

Technical Field

[0001] The present application relates to the field of holographic microscopic imaging, and specifically to an underwater micro-particle holographic microscopic imaging device. Background Art

[0002] Water pollution has become the most important water environment problem in my country. It not only exacerbates the tense situation of water shortage, but also seriously threatens human health and life safety. Among them, the outbreak of cyanobacteria blooms is a key issue of water environment protection in my country. Excessive reproduction of algae is the main cause of ecological disasters such as algae blooms. The establishment of a monitoring and early warning system for algae and other aquatic organisms will help reduce the risk and governance costs of cyanobacteria blooms, and is of great significance to water pollution prevention and control and lake chief management. As a new water environment monitoring technology, digital online holographic microscopy technology has the advantages of cleanliness, speed and convenience compared to traditional monitoring methods. It can obtain the size distribution of algae in water, the grading of sediment particles, etc., and can monitor water environment conditions such as microorganisms and sediment.

[0003] Laser holography has been used to measure microscopic particles in water for many years. However, the current technology has the following problems:

[0004] 1. The existing equipment is large in size and low in integration, and is not suitable for field working environments;

[0005] 2. The height of the microplate of the existing equipment cannot be flexibly adjusted, so the instantaneous fluid width of the measured target cannot be flexibly adjusted, and it cannot adapt to the measurement requirements of different particle densities;

[0006] Therefore, an underwater micro-particle holographic microscopic imaging device is now proposed. Utility Model Content

[0007] In order to improve the problems of existing micro-particle holographic microscopic imaging equipment being large in size, low in integration, and not being suitable for outdoor working environments, the utility model provides an underwater micro-particle holographic microscopic imaging device.

[0008] The utility model provides an underwater micro-particle holographic microscopic imaging device, which adopts the following technical solutions:

[0009] An underwater micro-particle holographic microscopic imaging device comprises an upper protective shell and an imaging adjustment component located below the upper protective shell;

[0010] The bottom of the upper protective shell is fixedly connected to the lower protective shell, a cold light CCD camera is fixedly installed inside the upper protective shell, a laser is installed inside the lower protective shell, and a micron-level pinhole is arranged on the top of the laser;

[0011] The imaging adjustment component includes an adjustment block, which is rotatably installed on one side of the lower protective shell, one end of the adjustment block is fixedly connected to a rotating shaft, the surface of the rotating shaft is fixedly connected to a second bevel gear, the top of the second bevel gear is meshed with a first bevel gear, and the top of the first bevel gear is fixedly connected to a screw rod.

[0012] As a further solution of the utility model, a microporous plate is threadedly connected to the surface of the screw rod.

[0013] As a further solution of the utility model, a timing synchronizer is fixedly installed on the inner top of the upper protective shell.

[0014] As a further solution of the utility model, an upper protective cover is fixedly installed on the top of the upper protective shell.

[0015] As a further solution of the utility model, two groups of first through holes are opened on the surface of the upper protective cover.

[0016] As a further solution of the utility model, a lower protective cover is fixedly installed on the bottom of the lower protective shell.

[0017] As a further solution of the utility model, a group of second through holes is opened on the surface of the lower protective cover.

[0018] As a further solution of the utility model, the upper protective cover and the lower protective cover are both made of 6061 aluminum alloy sheet and are anodized black.

[0019] As a further solution of the utility model, the upper protective shell and the lower protective shell are both made of 6061 aluminum alloy sheet and are anodized black.

[0020] As a further solution of the present invention, the aperture of the micron-sized pinhole is 1 μm.

[0021] Compared with the prior art, the utility model has the following beneficial effects:

[0022] 1. The utility model integrates the protective housing, imaging adjustment components, micron-level pinhole, cold light CCD camera, laser, and timing synchronizer into one, which is highly integrated, reduces the volume, is easy to carry, and is more suitable for field working environments;

[0023] 2. The utility model is provided with an imaging adjustment component. When in use, rotating the adjustment block can drive the rotating shaft to rotate, and then drive the second bevel gear, the first bevel gear and the screw to rotate, so as to flexibly adjust the height of the microplate. This setting is convenient for flexibly adjusting the instantaneous fluid width of the measured target, and ultimately meets the measurement requirements of different particle densities, reduces the work difficulty of the staff, and helps to capture the image and spectrum of the measured target. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of the protective shell of the utility model.

[0025] Figure 2 It is a schematic diagram of the structure inside the protective shell of the utility model.

[0026] Figure 3 It is a structural schematic diagram of the main view of the utility model.

[0027] Figure 4 It is a schematic diagram of the lower protective cover structure of the utility model.

[0028] Figure 5 It is a structural schematic diagram of the protective cover of the utility model.

[0029] Figure 6 This utility model Figure 3 Schematic diagram of the local structure.

[0030] Description of reference numerals:

[0031] 1. Lower protective shell; 2. Lower protective cover; 3. Upper protective cover; 4. First through hole; 5. Upper protective shell; 6. Imaging adjustment assembly; 601. Microplate; 602. Screw; 603. Adjustment block; 604. First bevel gear; 605. Rotating shaft; 606. Second bevel gear; 7. Micrometer-level pinhole; 8. Cold light CCD camera; 9. Laser; 10. Timing synchronizer; 11. Second through hole; 12. Battery compartment. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0033] In this document, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, the meaning of "plurality" is two or more.

[0034] In addition, in this article, directional terms such as "upper" and "lower" are defined relative to the orientation of the structure schematically placed in the drawings. It should be understood that these directional terms are relative concepts. They are used for relative description and clarification, and they can change accordingly according to the change of the orientation of the structure.

[0035] Please refer to Figure 1-Figure 6 , an underwater micro-particle holographic microscopic imaging device, comprising an upper protective shell 5, the bottom of which is fixedly connected to a lower protective shell 1, a cold light CCD camera 8 is fixedly installed inside the upper protective shell 5, a laser 9 is installed inside the lower protective shell 1, and a micron-level pinhole 7 is arranged at the top of the laser 9;

[0036] It is worth mentioning that a battery compartment 12 is installed inside the upper protective shell 5, and a lithium battery is installed inside the battery compartment. The outer periphery of the battery compartment is sealed and wrapped by a sealing cover to ensure waterproof sealing. The lithium battery can be used as a power source to supply power to the cold light CCD camera 8, the laser 9 and the main control board.

[0037] It also includes an imaging adjustment component 6 located below the upper protective shell 5; the imaging adjustment component 6 includes an adjustment block 603, the adjustment block 603 is rotatably mounted on one side of the lower protective shell 1, one end of the adjustment block 603 is fixedly connected to a rotating shaft 605, a second bevel gear 606 is fixedly connected to the surface of the rotating shaft 605, a first bevel gear 604 is meshed above the second bevel gear 606, a screw rod 602 is fixedly connected to the top of the first bevel gear 604, and a microplate 601 is threadedly connected to the surface of the screw rod 602;

[0038] It should be noted that a notch channel is set between the lower protective shell 1 and the upper protective shell 5, and the fluid flows through the notch channel. The particles therein are captured and photographed by the cold light CCD camera 8, and the microporous plate 601 is located at the notch channel. Through the setting of the imaging adjustment component 6, the position of the microporous plate 601 can be adjusted, so that the position of the microporous plate 601 entering the notch channel can be adjusted, and then the instantaneous fluid width in the notch channel can be changed, and finally the measurement requirements of different particle densities can be met.

[0039] A main control board is fixedly installed on the top of the upper protective shell 5, and a timing synchronizer 10 is installed on the main control board. The upper protective shell 5 and the lower protective shell 1 are both made of 6061 aluminum alloy plates, anodized and blackened, and the aperture of the micron-level pinhole 7 is 1μm. This setting improves the existing problems of low imaging resolution, poor imaging quality, and small reproduced depth of field, which cannot meet the problem of clear imaging of micro-plankton with a large depth of field of 2-20μm; the utility model adopts a design of high-collimation laser point light source + micron-level pinhole 7 + cold light CCD camera 8, and on this basis completes the optical path matching of laser energy, pinhole diameter and CCD camera parameters; in addition, an additional TTL-based timing synchronizer 10 is added to work in conjunction with the laser transmitter and the CCD camera to complete laser intensity control, ensure higher imaging quality, and achieve the effect of saving energy.

[0040] Reference Figure 1 , Figure 3 and Figure 4 An upper protective cover 3 is fixedly installed on the top of the upper protective shell 5, and two groups of first through holes 4 are opened on the surface of the upper protective cover 3. A lower protective cover 2 is fixedly installed on the bottom of the lower protective shell 1, and a group of second through holes 11 are opened on the surface of the lower protective cover 2. The upper protective cover 3 and the lower protective cover 2 are both made of 6061 aluminum alloy plate, and are anodized black. This arrangement improves the problems of high hardware cost of existing equipment and large size of existing equipment, which is not suitable for field working environment.

[0041] This application has at least one of the following advantages:

[0042] 1. In terms of hardware design, this underwater micro-particle holographic microscopic imaging device intends to adopt the design of high-collimation laser point light source + micron-level pinhole 7 + cold light CCD camera 8, on this basis, to complete the optical path matching of laser energy, pinhole diameter and CCD camera parameters; in addition, an additional TTL-based timing synchronizer 10 is added to work with the laser transmitter and CCD camera to complete the laser intensity control, ensure higher imaging quality, and achieve the effect of energy saving. At the same time, the protective shell, imaging adjustment component 6, micron-level pinhole 7, cold light CCD camera 8, laser 9, and timing synchronizer 10 are integrated into one, which is highly integrated, reduced in size, easy to carry, and more suitable for field working environments;

[0043] 2. By setting up the imaging adjustment component 6, when in use, rotating the adjustment block 603 can drive the rotating shaft 605 to rotate, and then drive the second bevel gear 606, the first bevel gear 604 and the screw 602 to rotate, so as to flexibly adjust the height of the microporous plate 601. Through the setting of the imaging adjustment component 6, the position of the microporous plate 601 can be adjusted, so that the position of the microporous plate 601 entering the notch channel can be adjusted, and then the instantaneous fluid width in the notch channel can be changed, and finally the measurement requirements of different particle densities can be met.

[0044] The underwater microparticle holographic microscopy imaging device provided by the implementation method of the present application is introduced in detail above, and the principle and implementation method of the present application are explained by using specific embodiments. The above description is only used to help understand the method of the present application and its core mechanism; at the same time, for general technical personnel in this field, according to the ideas of the present application, there will be changes in the specific embodiments and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. An underwater micro-particle holographic microscopic imaging device, characterized in that: It comprises an upper protective shell (5), and an imaging adjustment component (6) located below the upper protective shell (5); The bottom of the upper protective shell (5) is fixedly connected to the lower protective shell (1), a cold light CCD camera (8) is fixedly installed inside the upper protective shell (5), a laser (9) is installed inside the lower protective shell (1), and a micrometer-level pinhole (7) is arranged at the top of the laser (9); The imaging adjustment component (6) comprises an adjustment block (603), the adjustment block (603) being rotatably mounted on one side of the lower protective shell (1), one end of the adjustment block (603) being fixedly connected to a rotating shaft (605), a second bevel gear (606) being fixedly connected to the surface of the rotating shaft (605), a first bevel gear (604) being meshed above the second bevel gear (606), and a lead screw (602) being fixedly connected to the top of the first bevel gear (604).

2. The underwater micro-particle holographic microscopic imaging device according to claim 1, characterized in that: The surface of the screw rod (602) is threadedly connected to a microporous plate (601).

3. The underwater micro-particle holographic microscopic imaging device according to claim 2, characterized in that: A timing synchronizer (10) is fixedly mounted on the inner top of the upper protective housing (5).

4. The underwater micro-particle holographic microscopic imaging device according to claim 3, characterized in that: An upper protective cover (3) is fixedly mounted on the top of the upper protective shell (5).

5. The underwater micro-particle holographic microscopic imaging device according to claim 4, characterized in that: Two groups of first through holes (4) are provided on the surface of the upper protective cover (3).

6. The underwater micro-particle holographic microscopic imaging device according to claim 5, characterized in that: A lower protective cover (2) is fixedly mounted on the bottom of the lower protective shell (1).

7. The underwater micro-particle holographic microscopic imaging device according to claim 6, characterized in that: A group of second through holes (11) is provided on the surface of the lower protective cover (2).

8. The underwater micro-particle holographic microscopic imaging device according to claim 7, characterized in that: The upper protective cover (3) and the lower protective cover (2) are both made of 6061 aluminum alloy sheet material and are anodized black.

9. The underwater micro-particle holographic microscopic imaging device according to claim 8, characterized in that: The upper protective shell (5) and the lower protective shell (1) are both made of 6061 aluminum alloy sheet and are anodized black.

10. The underwater micro-particle holographic microscopic imaging device according to claim 9, characterized in that: The aperture of the micrometer-scale pinhole (7) is 1 μm.

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