A nucleic acid purification device

Abstract

The utility model discloses a nucleic acid purification device, comprising at least one group of purification components, a driving pump and a controller; each group of nucleic acid purification components is connected to the driving pump, and the driving pump is electrically connected to the controller; each group of purification components comprises a sample container, a microfluidic chip, a nucleic acid container, and a waste liquid container, wherein the sample container is connected to an inlet of the microfluidic chip, the inlet of the nucleic acid container and the inlet of the waste liquid container are intersected and connected to an outlet of the microfluidic chip, and the outlet of the nucleic acid container and the outlet of the waste liquid container are intersected and connected to the driving pump; an adsorption film is arranged in the microfluidic chip; the technical solution provided by the utility model controls the start and stop of the driving pump through the controller, and the driving pump provides driving force to multiple groups of nucleic acid purification components at the same time, thereby realizing nucleic acid purification of multiple samples at the same time, saving time and labor for nucleic acid purification, and improving nucleic acid detection efficiency.

Description

Technical Field

[0001] The utility model relates to the field of molecular biology detection, in particular to a nucleic acid purification device. Background Art

[0002] Nucleic acid is one of the most basic substances in organisms. It can be divided into two categories: DNA and RNA. It is widely present in all organisms, including animals, plant cells, and microorganisms. Nucleic acid testing uses the theory and technology of molecular biology to amplify the gene sequence of pathogenic microorganisms and observe fluorescent signals to determine whether specific pathogenic microorganisms are present.

[0003] In order to avoid false negatives during nucleic acid testing, purification of the nucleic acid of pathogenic microorganisms in the sample to be tested is an important step in nucleic acid testing. The nucleic acid purification process can remove interfering substances, prevent interfering substances from inhibiting nucleic acid reactions, avoid false negatives, and ensure the accuracy and reliability of test results.

[0004] Existing nucleic acid purification devices can only purify the nucleic acid of one sample to be tested at a time, and cannot purify multiple nucleic acid samples at the same time. When multiple nucleic acid samples need to be tested, the staff can only purify the nucleic acid of one sample at a time, which is time-consuming and labor-intensive, resulting in low detection efficiency. Therefore, developing a device that can purify nucleic acid from multiple samples at the same time is an urgent problem to be solved. Utility Model Content

[0005] The purpose of the utility model is to provide a nucleic acid purification device to solve the problems existing in the above-mentioned prior art. A driving pump is controlled by a controller, and the driving pump provides driving force to multiple sets of nucleic acid purification components at the same time, thereby realizing nucleic acid purification of multiple samples at the same time, saving time and labor for nucleic acid purification, and improving the efficiency of nucleic acid detection.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A nucleic acid purification device comprises at least one group of purification components, a driving pump and a controller; each group of the nucleic acid purification components is connected to the driving pump, and the driving pump is electrically connected to the controller; each group of the purification components comprises a sample container, a microfluidic chip, a nucleic acid container, and a waste liquid container, wherein the sample container is connected to the inlet of the microfluidic chip, the inlet of the nucleic acid container and the inlet of the waste liquid container are connected to the outlet of the microfluidic chip, and the outlet of the nucleic acid container and the outlet of the waste liquid container are connected to the driving pump; an adsorption membrane is arranged in the microfluidic chip.

[0008] Preferably, a first solenoid valve is provided between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the waste liquid container.

[0009] Preferably, a ventilation valve is provided between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the nucleic acid container.

[0010] Preferably, a second solenoid valve is provided between the intersection of the nucleic acid container outlet and the waste liquid container outlet and the nucleic acid container.

[0011] Preferably, the adsorption film is a silicon-based film.

[0012] Preferably, the sample container inlet is connected to a pre-filter, and filter material is arranged in the pre-filter.

[0013] Preferably, the pre-filter is provided with a first solution inlet, a second solution inlet and a solution outlet, the first solution inlet is arranged above the filter material, the second solution inlet is arranged below the filter material, and the solution outlet is connected to the inlet of the sample container.

[0014] Preferably, the filter material is one or more combinations of medical stone, zeolite and fiber membrane.

[0015] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0016] The nucleic acid purification device disclosed by the utility model connects a sample container to a microfluidic chip, an adsorption membrane for adsorbing nucleic acid is arranged in the microfluidic chip, and an inlet of the nucleic acid container and an inlet of a waste liquid container are connected to an outlet of the microfluidic chip, and an outlet of the nucleic acid container and an outlet of the waste liquid container are connected to a driving pump. The start and stop of the driving pump is controlled by a controller, and the driving pump provides driving force to multiple groups of nucleic acid purification components at the same time, thereby realizing nucleic acid purification of multiple samples at the same time, saving time and labor for nucleic acid purification, and improving nucleic acid detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 This is a structural diagram of a nucleic acid purification device;

[0019] Figure 2 It is a schematic diagram of the structure of the pre-filter;

[0020] Figure 3 This is a structural block diagram of the nucleic acid purification device provided in Example 3;

[0021] Among them, 1 is the first solution inlet; 2 is the second solution inlet; 3 is the solution outlet; and 10 is the filter material. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] The utility model aims to provide a nucleic acid purification device to solve the problems existing in the above-mentioned prior art, realize nucleic acid purification of multiple samples at the same time, save time and labor for nucleic acid purification, and improve the efficiency of nucleic acid detection.

[0024] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods. Embodiment 1

[0025] This embodiment provides a nucleic acid purification device, such as Figure 1 and Figure 2 As shown, it includes at least one group of purification components, a driving pump and a controller; each group of nucleic acid purification components is connected to the driving pump, and the driving pump is electrically connected to the controller; each group of purification components includes a sample container, a microfluidic chip, a nucleic acid container, and a waste liquid container, wherein the sample container is connected to the inlet of the microfluidic chip, the inlet of the nucleic acid container and the inlet of the waste liquid container are connected to the outlet of the microfluidic chip, and the outlet of the nucleic acid container and the outlet of the waste liquid container are connected to the driving pump; an adsorption membrane is arranged in the microfluidic chip.

[0026] In the nucleic acid purification device provided in this embodiment, the number of purification components corresponds to the number of driving pumps, and each group of nucleic acid purification components is connected to a corresponding driving pump.

[0027] When using this embodiment, the specific steps include:

[0028] Step 1, extracting the lysed sample: connecting the pipeline where the sample container-microfluidic chip-waste liquid container-driving pump is located, disconnecting the pipeline between the nucleic acid container and the driving pump, adding multiple lysed samples into the corresponding sample containers one by one, and the controller controls the driving pump to start. At this time, the driving pump draws negative pressure on multiple waste liquid containers. The lysate nucleic acid sample in the sample container flows into the microfluidic chip under the drive of negative pressure, and the nucleic acid is adsorbed on the adsorption membrane provided in the microfluidic chip. The lysate waste liquid enters the waste liquid container under the drive of negative pressure. After all the lysate flows into the waste liquid container, the controller controls the driving pump to close;

[0029] Step 2, pumping washing liquid: adding washing liquid into corresponding sample containers one by one, the controller controls the driving pump to start, at this time the driving pump pumps negative pressure to multiple waste liquid containers, the washing liquid in the sample container flows into the microfluidic chip under the driving of negative pressure, in this process, the impurities on the adsorption membrane are eluted by the washing liquid, the nucleic acid is still adsorbed on the adsorption membrane, the washing liquid and impurities enter the waste liquid container under the driving of negative pressure, after the washing liquid has all flowed into the waste liquid container, the controller controls the driving pump to close;

[0030] Step three, extracting the eluent: connect the pipelines of sample container-microfluidic chip-nucleic acid container-driving pump, disconnect the pipeline between the waste liquid container and the driving pump, add the eluent to the corresponding sample containers one by one, and the controller controls the driving pump to start. At this time, the driving pump draws negative pressure on multiple nucleic acid containers, and the eluent in the sample container flows into the microfluidic chip under the drive of negative pressure. In this process, the nucleic acid adsorbed on the adsorption membrane is eluted by the eluent, and the nucleic acid and the eluent enter the nucleic acid container together under the drive of negative pressure. After all the eluent flows into the nucleic acid container, the controller controls the driving pump to close, and multiple purified nucleic acid samples are collected one by one in multiple nucleic acid containers.

[0031] It can be seen that the technical solution provided by the utility model connects the sample container to the microfluidic chip, an adsorption membrane for adsorbing nucleic acids is arranged in the microfluidic chip, and the inlet of the nucleic acid container and the inlet of the waste liquid container are connected to the outlet of the microfluidic chip, and the outlet of the nucleic acid container and the outlet of the waste liquid container are connected to the driving pump. The start and stop of the driving pump is controlled by the controller, and the driving pump provides driving force to multiple groups of nucleic acid purification components at the same time, thereby realizing nucleic acid purification of multiple samples at the same time, saving time and labor for nucleic acid purification, and improving the efficiency of nucleic acid detection.

[0032] Based on the above principle, the nucleic acid purification device in this embodiment only needs a controller, a driving pump, a sample container, a microfluidic chip, an adsorption membrane, a nucleic acid container and a waste liquid container to purify nucleic acids of multiple samples at the same time, making nucleic acid purification time-saving and labor-saving. The solution provided in this embodiment does not limit the other structures.

[0033] The driving pump provided in this embodiment can be a peristaltic pump or a vacuum pump. In order to simplify the nucleic acid purification device, the preferred driving pump in this embodiment is a vacuum pump.

[0034] In some embodiments, in order to facilitate full-automatic control and avoid the situation in which, during the nucleic acid purification process, contaminated aerosols in the waste liquid container are drawn into the nucleic acid container and contaminate the purified nucleic acid when the nucleic acid is eluted into the nucleic acid container, a first solenoid valve is provided between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and between the waste liquid container.

[0035] In this embodiment, when extracting the sample solution and washing solution after lysis, the controller controls the first solenoid valve to be in an open state; when extracting the elution liquid, the controller controls the first solenoid valve to be in a closed state. This avoids the contaminated aerosol in the waste liquid container from being extracted into the nucleic acid container when eluting the nucleic acid, prevents the aerosol contamination in the waste liquid container from being cross-linked to the nucleic acid container and contaminating the nucleic acid, thereby ensuring the accuracy and reliability of subsequent nucleic acid detection results.

[0036] In some embodiments, when extracting the sample solution and washing solution after the lysis solution, in order to prevent the nucleic acid container from being in a negative pressure state, a ventilation valve is provided between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the nucleic acid container.

[0037] In this embodiment, when the step of extracting the sample solution and the washing solution after the lysis is performed, the controller controls the ventilation valve to be in the open state. Such a design avoids the nucleic acid container being in a negative pressure state during the step of extracting the sample solution and the washing solution after the lysis, and avoids the negative pressure in the nucleic acid container from quickly sucking the eluent into the nucleic acid container during the next step of extracting the eluent, thereby preventing the eluent from flowing through the adsorption membrane too fast, thereby causing the nucleic acid on the adsorption membrane to be unable to be eluted into the nucleic acid container by the eluent; by arranging a ventilation valve between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the nucleic acid container, it is ensured that the nucleic acid on the adsorption membrane can be driven by the pump. The uniform negative pressure is pumped into the nucleic acid container, thereby ensuring that the nucleic acid sample will not be lost and ensuring the accuracy and reliability of the subsequent nucleic acid detection results.

[0038] In some embodiments, in order to facilitate fully automated control, there is no need to manually disconnect or connect the pipeline between the nucleic acid container and the drive pump. At the same time, when extracting the sample solution and washing solution after the lysis solution, in order to avoid the nucleic acid container being in a negative pressure state, a second solenoid valve is provided between the intersection of the nucleic acid container outlet and the waste liquid container outlet and the nucleic acid container.

[0039] In this embodiment, when extracting the sample solution and washing solution after lysis, the controller controls the ventilation valve to be open and the second solenoid valve to be closed, which not only avoids the nucleic acid container from being in a negative pressure state, but also realizes full automation of the nucleic acid purification process.

[0040] In some embodiments, in order to simplify the nucleic acid purification device, the adsorption membrane is a silicon-based membrane.

[0041] In some embodiments, the inlet of the sample container is connected to a pre-filter, in which a filter material 10 is disposed. The pre-filter is used to remove some large particles contained in the sample after lysis to prevent clogging of the adsorption membrane.

[0042] In some embodiments, please refer to Figure 2In order to separate the inlet of the sample solution and the washing solution after lysis from the inlet of the eluent, so that the eluent does not flow through the place where the sample solution and the washing solution after lysis flow, and to ensure the cleanliness of the eluent, the pre-filter is provided with a first solution inlet 1, a second solution inlet 2 and a solution outlet 3, the first solution inlet 1 is arranged above the filter material 10, the second solution inlet 2 is arranged below the filter material 10, and the solution outlet 3 is connected to the inlet of the sample container.

[0043] In this embodiment, the lysed sample solution and washing solution are added from the first solution inlet 1, and the eluent is added from the second solution inlet 2. With this design, the eluent is added from a separate inlet, and the eluent does not pass through the filter material 10, thereby avoiding the contamination of the eluent by the residual lysate and ethanol in the filter material 10, ensuring the cleanliness of the eluent, and making the subsequent nucleic acid detection experimental results accurate and reliable.

[0044] In some embodiments, in order to simplify the nucleic acid purification device, the filter material 10 is one or more combinations of medical stone, zeolite and fiber membrane. Embodiment 2

[0045] This example describes the method of using the nucleic acid purification device provided in Example 1. Please refer to Figures 1 to 3 The method for using the nucleic acid purification device provided in Example 1 is as follows:

[0046] Step 1, pumping the lysed sample solution through the membrane: adding the lysed sample solutions one by one into the corresponding first solution inlet 1; the controller controls the first solenoid valve and the ventilation valve to open, and controls the second solenoid valve to close; the controller controls the corresponding multiple driving pumps to open, and the lysed sample solution enters the sample container, and the lysed nucleic acid sample in the sample container flows into the microfluidic chip under the drive of negative pressure, and the nucleic acid is adsorbed on the adsorption membrane set in the microfluidic chip, and the lysed waste liquid enters the waste liquid container under the drive of negative pressure; after all the lysed liquid flows into the waste liquid container, the controller controls the corresponding multiple driving pumps to close;

[0047] Step 2, pumping washing liquid through the membrane: the washing liquid is added to the corresponding first solution inlet 1 one by one, and the controller controls the corresponding multiple driving pumps to start, and the washing liquid enters the sample container. The washing liquid in the sample container flows into the microfluidic chip under the driving of negative pressure. In this process, the impurities on the adsorption membrane are eluted by the washing liquid, and the nucleic acid is still adsorbed on the adsorption membrane. The washing liquid and impurities enter the waste liquid container under the driving of negative pressure. After the washing liquid has all flowed into the waste liquid container, the controller controls the driving pump to close;

[0048] Step three, pumping the eluent through the membrane: adding the eluents one by one into the corresponding second solution inlet 2, the lysate enters the sample container, the controller controls the first solenoid valve and the ventilation valve to close, and controls the second solenoid valve to open; the controller controls the corresponding multiple driving pumps to start, at this time the driving pumps pump negative pressure to the multiple nucleic acid containers, the eluent in the sample container flows into the microfluidic chip under the drive of the negative pressure, in this process, the nucleic acid adsorbed on the adsorption membrane is eluted by the eluent, the nucleic acid and the eluent enter the nucleic acid container together under the drive of the negative pressure, after the eluent has all flowed into the nucleic acid container, the controller controls the multiple driving pumps and the multiple second solenoid valves to close, and the multiple purified nucleic acid samples are collected one by one in the multiple nucleic acid containers. Embodiment 3

[0049] This embodiment provides another nucleic acid purification device, such as Figure 3 As shown, Figure 3 The same driving pump is used to provide driving force for multiple groups of nucleic acid purification devices.

[0050] This embodiment has all the advantages described in the first embodiment, which will not be described in detail here.

[0051] like Figure 3 As shown, the difference from the first embodiment is the number of driving pumps. In this embodiment, multiple groups of nucleic acid purification devices are all driven by the same driving pump. When multiple samples of the same type need to be purified, the nucleic acid purification device provided by this embodiment can be used. One driving pump can simultaneously draw negative pressure on multiple groups of nucleic acid purification components. In order to facilitate the control of several of the multiple groups of nucleic acid purification devices, a third solenoid valve can be further arranged between the intersection of the second solenoid valve and the waste liquid container outlet and the driving pump.

[0052] The present invention uses specific examples to illustrate the principle and implementation of the present invention. The above examples are only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A nucleic acid purification device, characterized in that: It comprises at least one group of purification components, a driving pump and a controller; each group of the nucleic acid purification components is connected to the driving pump, and the driving pump is electrically connected to the controller; each group of the purification components comprises a sample container, a microfluidic chip, a nucleic acid container, and a waste liquid container, wherein the sample container is connected to the inlet of the microfluidic chip, the inlet of the nucleic acid container and the inlet of the waste liquid container are connected to the outlet of the microfluidic chip, and the outlet of the nucleic acid container and the outlet of the waste liquid container are connected to the driving pump; an adsorption membrane is arranged in the microfluidic chip.

2. The nucleic acid purification device according to claim 1, characterized in that: A first electromagnetic valve is arranged between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the waste liquid container.

3. The nucleic acid purification device according to claim 2, characterized in that: A ventilation valve is provided between the intersection of the inlet of the nucleic acid container and the inlet of the waste liquid container and the nucleic acid container.

4. The nucleic acid purification device according to claim 3, characterized in that: A second electromagnetic valve is arranged between the intersection of the nucleic acid container outlet and the waste liquid container outlet and the nucleic acid container.

5. The nucleic acid purification device according to claim 1, characterized in that: The adsorption film is a silicon-based film.

6. The nucleic acid purification device according to claim 1, characterized in that: The sample container inlet is connected to a pre-filter, and filter material is arranged in the pre-filter.

7. The nucleic acid purification device according to claim 6, characterized in that: The pre-filter is provided with a first solution inlet, a second solution inlet and a solution outlet, wherein the first solution inlet is arranged above the filter material, the second solution inlet is arranged below the filter material, and the solution outlet is communicated with the inlet of the sample container.

8. The nucleic acid purification device according to claim 6, characterized in that: The filter material is one or more combinations of medical stone, zeolite and fiber membrane.

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