Gene sequencer and mobile liquid supply device

Abstract

The utility model discloses a gene sequencer and a mobile liquid supply device. The gene sequencer includes a frame, a chip motion platform, a liquid circuit system, a rotary valve seat, a valve seat driving mechanism and a control system. The chip motion platform includes a chip seat and a chip seat driving mechanism. The liquid circuit system is used to communicate with the sequencing flow channel of the sequencing chip; the liquid circuit system includes a rotary valve, and the rotary valve is used to select a target sample and / or a target reagent to be injected into the sequencing flow channel. The rotary valve seat is movably configured on the frame, and the rotary valve is configured on the rotary valve seat. When the chip motion platform drives the sequencing chip to move, the valve seat driving mechanism can drive the rotary valve seat to follow the chip seat to move, thereby shortening the length of the chip liquid supply pipeline between the rotary valve and the sequencing chip, thereby reducing the reagent loss, and solving the technical problem of large reagent loss in the current gene sequencer.

Description

Technical Field

[0001] The utility model relates to the technical field of gene sequencing, and in particular to a gene sequencer and a mobile liquid supply device. Background Art

[0002] Gene sequencing equipment can identify and sort the base sequence of sample DNA. Current sequencers usually include a chip platform, and the sequencing chip is installed on the chip platform. The sequencer includes a liquid system, and the liquid system provides samples and / or reagents to the sequencing chip. Due to the large variety of samples and reagents, in order to simplify the pipeline of the liquid system, the liquid system usually includes a rotary valve, and the liquid flowing out of the rotary valve enters the sequencing chip. The rotary valve can switch the type of liquid flowing into the sequencing chip. During the sequencing process of the sequencing chip, it is necessary to take a picture of the chip. When taking pictures, the chip platform needs to move with the chip. In order to ensure that the liquid pipeline between the rotary valve and the sequencing chip adapts to the position of the sequencing chip, the liquid pipeline between the rotary valve and the sequencing chip usually needs to have a redundant length, and the excessive redundant length causes excessive reagent loss, and there is a technical problem of large reagent loss. Utility Model Content

[0003] The present application provides a gene sequencer and a mobile liquid supply device, which are used to solve the technical problem of large reagent loss in current gene sequencers.

[0004] In one embodiment, a gene sequencer is provided, comprising:

[0005] frame;

[0006] A chip motion platform, the chip motion platform comprising a chip seat and a chip seat driving mechanism, the chip seat is used to mount a sequencing chip, the chip seat is movably configured on the frame, and the chip seat driving mechanism is used to drive the chip seat to move;

[0007] A liquid circuit system, the liquid circuit system is connected to the sequencing channel of the sequencing chip; the liquid circuit system includes a first rotary valve, the first rotary valve is used to select a target sample and / or a target reagent to be injected into the sequencing channel; the liquid circuit system also includes a chip liquid supply pipeline, the chip liquid supply pipeline is used to be connected between the first rotary valve and the sequencing chip;

[0008] A rotary valve seat, the rotary valve seat is movably arranged on the frame, and the first rotary valve is arranged on the rotary valve seat;

[0009] A valve seat driving mechanism, the valve seat driving mechanism is used to drive the rotary valve seat to move;

[0010] And a control system, wherein the control system is connected to the valve seat driving mechanism and the chip seat driving mechanism, and is used to control the rotary valve seat to follow the movement of the chip seat.

[0011] In one embodiment, the liquid circuit system includes a second rotary valve; at least part of the liquid inlet of the first rotary valve is connected to the second rotary valve so that the second rotary valve can supply liquid to the first rotary valve; and the second rotary valve is arranged on the rotary valve seat.

[0012] In one embodiment, the rotary valve seat is configured on the frame to move along a first direction, the first rotary valve and the second rotary valve are arranged side by side in a second direction, and an angle between the first direction and the second direction is greater than 0 degrees and not greater than 90 degrees.

[0013] In one embodiment, the rotary valve seat is movably configured on the frame along a first direction, the first rotary valve is in the shape of an elongated strip extending along the first direction, the second rotary valve is in the shape of an elongated strip extending along a second direction, and the angle between the first direction and the second direction is greater than 0 degrees and not greater than 90 degrees; the liquid path interface of the first rotary valve is located at one end of the first rotary valve that is close to the sequencing chip.

[0014] In one embodiment, the liquid circuit system includes a second rotary valve; part of the liquid inlet of the first rotary valve is connected to the second rotary valve so that the second rotary valve can supply liquid to the first rotary valve; and the second rotary valve is arranged on the frame.

[0015] In one embodiment, the rotary valve seat is configured on the frame for movement along a first direction, the control system can control the rotary valve seat to follow the movement of the chip seat in the first direction, the chip movement platform includes a moving seat, the moving seat is configured on the frame for movement along a first direction, and the chip seat is configured on the moving seat for movement along a second direction, so that the chip seat moves along the first direction and the second direction.

[0016] In one embodiment, the first direction is used to be consistent with the extension direction of the sequencing channel. During the sequencing process of the sequencing chip, the moving distance of the movable seat in the first direction is greater than the moving distance of the chip seat in the second direction, and the rotary valve seat can only reciprocate along the first direction.

[0017] In one embodiment, the valve seat driving mechanism includes a driving wheel, a driven wheel and a transmission belt, the driving wheel and the driven wheel are arranged at intervals along the first direction, the transmission belt is wound around the driving wheel and the driven wheel, and the rotating valve seat is connected to the transmission belt so that the driving wheel can drive the rotating valve seat to move through the transmission belt.

[0018] In one embodiment, the frame includes a column and a longitudinal beam fixed on the column, the longitudinal beam extends along the first direction, and the rotary valve seat is movably arranged on a portion of the longitudinal beam overhanging one side of the column.

[0019] In one embodiment, a mobile liquid supply device is provided, comprising:

[0020] frame;

[0021] A first rotary valve, which is used to select a target sample and / or a target reagent and inject it into a sequencing flow channel of a sequencing chip;

[0022] A rotary valve seat, the rotary valve seat is movably arranged on the frame, and the first rotary valve is arranged on the rotary valve seat;

[0023] and a valve seat driving mechanism, used for driving the rotary valve seat to move so that the first rotary valve can follow the movement of the sequencing chip.

[0024] In one embodiment, the mobile liquid supply device includes a second rotary valve; part of the liquid inlet of the first rotary valve is connected to the second rotary valve so that the second rotary valve can supply liquid to the first rotary valve; and the second rotary valve is arranged on the rotary valve seat.

[0025] According to the gene sequencer and mobile liquid supply device of the above-mentioned embodiments, since the rotary valve seat is movably configured on the frame and the first rotary valve is configured on the rotary valve seat, when the chip motion platform drives the sequencing chip to move, the valve seat driving mechanism can drive the rotary valve seat to follow the movement of the chip seat, thereby shortening the length of the chip liquid supply pipeline between the first rotary valve on the rotary valve seat and the sequencing chip, thereby reducing the reagent loss and solving the technical problem of large reagent loss in the current gene sequencer. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of the chip motion platform in an embodiment;

[0027] Figure 2 A schematic diagram of the structure of the chip motion platform at another viewing angle in one embodiment;

[0028] Figure 3 A top view of the chip moving platform in one embodiment;

[0029] Figure 4 for Figure 3 Right view of;

[0030] Figure 5 This is a schematic diagram of the structure of the chip motion platform of the second gene sequencer in an embodiment;

[0031] Figure 6 The figure is a schematic diagram of the liquid circuit of a gene sequencer in one embodiment.

[0032] The reference numerals are as follows:

[0033] 1-frame; 11-frame side panel; 12-column; 13-longitudinal beam; 14-guide rail; 2-chip motion platform; 21-chip seat; 211-sequencing chip installation station; 22-moving seat; 3-rotating valve seat; 31-crossbeam; 32-fixed frame; 4-valve seat drive mechanism; 41-driving wheel; 42-driven wheel; 43-transmission belt; 44-driving motor; 5-rotary valve; 5a-first rotary valve; 51a-liquid circuit interface; 5b-second rotary valve; 51b-liquid circuit interface; 6-liquid circuit system; 61-chip liquid supply pipeline; 62-pipeline; 7-sequencing chip; 71-sequencing flow channel; 8-reactor kit; 9-liquid inlet block; 10-liquid outlet block; 11-injection pump; 12-merging block; 13-waste liquid barrel. DETAILED DESCRIPTION

[0034] The present invention is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are intended to enable the present application to be better understood. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0035] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a required sequence, unless otherwise specified that a certain sequence must be followed.

[0036] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0037] In current gene sequencers, in order to adapt to the movement of the sequencing chip, the pipeline connected to the sequencing chip is usually designed with a long redundant length, resulting in a large loss of reagents. In the gene sequencer proposed in this application, the length of the chip liquid supply pipeline between the rotary valve and the sequencing chip is shortened by making the rotary valve follow the movement of the chip seat.

[0038] In one embodiment, please refer to Figures 1 to 6 The gene sequencer includes a frame 1, a chip motion platform 2, a rotary valve seat 3, a valve seat driving mechanism 4, a liquid circuit system 6 and a control system (not shown in the figure).

[0039] The chip moving platform 2 is used to carry the sequencing chip 7 (please refer to Figure 6 ), and drives the sequencing chip 7 to move so as to sequentially photograph the analytes in the multiple sequencing channels 71 in the sequencing chip 7. The sequencing chip 7 is a flat plate structure, and the top surface of the sequencing chip 7 has multiple parallel sequencing channels 71. An inlet is provided at one end of the sequencing chip 7, and an outlet is provided at the other end. Each sequencing channel 71 may be provided with an inlet and an outlet respectively, or multiple sequencing channels 71 may share an inlet and an outlet. The sequencing channel 71 is used to provide a place for the reaction or incubation of the analyte. For example, in the gene sequencing process, the sequencing channel 71 provides a place for the reaction of biological samples and reagents.

[0040] The chip motion platform 2 includes a chip seat 21 and a chip seat driving mechanism (not shown in the figure), and the sequencing chip 7 is mounted on the chip seat 21. In order to realize the movement of the sequencing chip 7, the chip seat 21 is movably configured on the frame 1, and the chip seat driving mechanism is used to drive the chip seat 21 to move. In one embodiment, the chip seat 21 is movably configured on the frame 1, and the chip seat driving mechanism can be a driving mechanism such as a cylinder or a linear motor.

[0041] The liquid system 6 is connected to the sequencing flow channel 71 of the sequencing chip 7, and liquid can be supplied to the sequencing chip 7 through the liquid system 6. The liquid system 6 includes a rotary valve 5 and a chip liquid supply pipeline 61 (please refer to Figure 6 ), at least one rotary valve is a first rotary valve 5a, the first rotary valve 5a is used to select a target sample and / or a target reagent to be injected into a sequencing channel 71, and the chip liquid supply pipeline 61 is used to be connected between the first rotary valve 5a and the sequencing chip 7.

[0042] In order to shorten the length of the chip liquid supply pipeline 61, the rotary valve seat 3 of the present application is movably arranged on the frame 1, the valve seat driving mechanism 4 is used to drive the rotary valve seat 3 to move, and the first rotary valve 5a is arranged on the rotary valve seat 3. The control system is connected to the valve seat driving mechanism 4 and the chip seat driving mechanism to control the rotary valve seat 3 to follow the movement of the chip seat 21.

[0043] In the present application, the first rotary valve 5a is arranged on the rotary valve seat 3 by movably configuring the rotary valve seat 3 on the frame 1 so that it can follow the movement of the sequencing chip 7. When the chip motion platform 2 drives the sequencing chip 7 to move, the valve seat driving mechanism 4 can drive the first rotary valve 5a on the rotary valve seat 3 to follow the movement of the chip seat 21, thereby shortening the length of the chip liquid supply pipeline 61 between the first rotary valve 5a on the rotary valve seat 3 and the sequencing chip 7, thereby reducing reagent loss.

[0044] In one embodiment, please refer to Figure 6 The liquid path system 6 also includes a liquid inlet block 9 located upstream of the sequencing chip 7 , a liquid outlet block 10 located downstream of the sequencing chip 7 , a syringe pump 11 , a confluence block 12 and a waste liquid bucket 13 .

[0045] It should be noted that in the present application, the rotating valve seat 3 follows the movement of the chip seat 21, which not only includes the synchronous movement of the rotating valve seat 3 and the chip seat 21, but also includes the synchronous movement of the rotating valve seat 3 and the chip seat 21 in a certain direction, and relative movement in other directions. It can be understood that the rotating valve seat 3 follows the movement of the chip seat 21, which can be synchronous or asynchronous. Compared with the situation where the rotating valve seat 3 is fixed and does not follow the movement of the chip seat 21, when following asynchronously, the distance between the rotating valve seat 3 and the chip seat 21 can gradually increase, but the increase speed is slow, which can also reduce the redundant length of the chip liquid supply pipeline 61. Of course, asynchronous following also includes the situation where the distance between the rotating valve seat 3 and the chip seat 21 becomes smaller. At this time, the movement speed of the rotating valve seat 3 can be greater than the movement speed of the chip seat 21 at certain moments.

[0046] In one embodiment, please refer to Figures 1 to 5 , the number of rotary valves 5 in the liquid path system 6 is at least two. The specific number of rotary valves 5 can be increased or decreased as needed, for example, it can be 1 or more than two. When the number of rotary valves 5 is more than two, each rotary valve 5 can be the first rotary valve 5a supplying liquid to the sequencing chip 7, and at this time, the chip liquid supply pipeline 61 can have multiple; each rotary valve 5 can also partially be the first rotary valve 5a supplying liquid to the sequencing chip 7, and the remaining rotary valves 5 supply liquid to the first rotary valve 5a. In one embodiment, please refer to Figure 1 , at least one rotary valve 5 is a first rotary valve 5a, and at least one rotary valve 5 is a second rotary valve 5b. The liquid path interface 51a (not fully shown in the figure) of the first rotary valve 5a includes a liquid inlet and a liquid outlet, wherein the liquid outlet is connected to the chip liquid supply pipeline 61, and at least part of the liquid inlet of the first rotary valve 5a is connected to the second rotary valve 5b, so that the second rotary valve 5b can supply liquid to the first rotary valve 5a. In one embodiment, please refer to Figure 6, part of the liquid inlets of the first rotary valve 5a are directly connected to the reagent kit 8, so that the reagent or sample is directly supplied to the sequencing chip 7 through the first rotary valve 5a. In another embodiment, all the liquid inlets of the first rotary valve 5a are connected to the second rotary valve 5b, and the number of the second rotary valves 5b can be one or more than two.

[0047] In one embodiment, please refer to Figure 1 and Figure 2 , the number of the first rotary valve 5a and the number of the second rotary valve 5b are both two, there are two sequencing chip installation stations 211 on the chip holder 21, the two first rotary valves 5a correspond to the two sequencing chip installation stations 211 one by one, and one first rotary valve 5a supplies liquid to the sequencing chip 7 of one sequencing chip installation station 211. The two first rotary valves 5a correspond to the two second rotary valves 5b one by one, and the second rotary valve 5b can supply liquid to the corresponding first rotary valve 5a.

[0048] In one embodiment, please refer to Figure 1 , the first rotary valve 5a is arranged on the rotary valve seat 3, and the chip liquid supply pipeline 61 is connected between the first rotary valve 5a and the sequencing chip 7, so that when the chip motion platform 2 drives the sequencing chip 7 to move, the first rotary valve 5a moves with the sequencing chip 7 driven by the rotary valve seat 3. Part of the liquid inlet of the first rotary valve 5a is connected to the second rotary valve 5b through a pipeline. In order to reduce the length of the pipeline 62 between the second rotary valve 5b and the first rotary valve 5a, the second rotary valve 5b is also arranged on the rotary valve seat 3 to move with the rotary valve seat 3. The first rotary valve 5a and the second rotary valve 5b are both arranged on the rotary valve seat 3 to make the overall structure more compact.

[0049] In one embodiment, please refer to Figure 1 The rotary valve seat 3 is configured on the frame 1 to move along the first direction, and the control system can control the rotary valve seat 3 to move along the chip seat 21 in the first direction. In one embodiment, the first direction is used to be consistent with the extension direction of the sequencing flow channel 71.

[0050] It should be noted that the control system at this time controls the first rotary valve 5a in the following manner: since the control system is connected to the valve seat drive mechanism 4 and the chip seat drive mechanism, the control system controls the operation of the chip seat drive mechanism by controlling the operation of the valve seat drive mechanism 4 and the chip seat drive mechanism at the same time, thereby controlling the movement of the chip seat 21 and the rotary valve seat 3. For example, when the chip seat 21 needs to move in the first direction, the control system controls the chip seat drive mechanism to work, driving the chip seat 21 to move in the first direction, and at the same time, the control system controls the valve seat drive mechanism 4 to work, driving the rotary valve seat 3 to move in the first direction and following the movement of the chip seat 21. The valve seat drive mechanism 4 and the chip seat drive mechanism include a motor, a cylinder or an oil cylinder that provide power, and the control system is connected to the motor, the cylinder or the oil cylinder by wire or wirelessly to control them.

[0051] In one embodiment, the rotary valve seat 3 can be configured on the frame 1 in addition to being movably disposed, and can also be configured in other ways of movement, for example, the rotary valve seat 3 can be configured on the frame 1 in a swinging manner.

[0052] In one embodiment, please refer to Figures 1 to 4 The rotary valve seat 3 is moved along the first direction and arranged on the frame 1, and the first rotary valve 5a and the second rotary valve 5b are arranged side by side in the second direction. In addition to the side-by-side arrangement, in one embodiment, please refer to Figure 5 The first rotary valve 5a is in the shape of a long strip extending along a first direction, and the second rotary valve 5b is in the shape of a long strip extending along a second direction. The angle between the first direction and the second direction is greater than 0 degree and not greater than 90 degrees.

[0053] In one embodiment, in order to shorten the length of the pipeline, please refer to Figures 1 to 4 The liquid path interface 51a of the first rotary valve 5a is located at one end of the first rotary valve 5a that is close to the sequencing chip 7, so that the distance between the liquid path interface 51a of the first rotary valve 5a and the sequencing chip 7 is shorter, thereby reducing the length of the chip liquid supply pipeline 61.

[0054] Similarly, the liquid circuit interface 51b of the second rotary valve 5b (not fully shown in the figure) is located at one end of the second rotary valve 5b that is close to the liquid circuit interface 51a of the first rotary valve 5a, so that the liquid circuit interface 51b of the second rotary valve 5b is closer to the liquid circuit interface 51a of the first rotary valve 5a.

[0055] In one embodiment, please refer to Figure 1 and Figure 5 , the first direction is perpendicular to the second direction. In addition to being perpendicular to the second direction, the angle between the first direction and the second direction may also be any value greater than 0 degrees and less than 90 degrees.

[0056] In one embodiment, please refer to Figure 5, the second rotary valve 5b is not arranged on the rotary valve seat 3, but on the frame 1. In this case, the pipeline 62 between the second rotary valve 5b and the first rotary valve 5a needs to be provided with a redundant length. Please refer to Figure 5 , the first rotary valve 5a and the second rotary valve 5b are arranged vertically.

[0057] Please refer to Figure 5 The rack 1 includes rack side plates 11 spaced apart in the second direction, and the second rotary valve 5b is fixed on the rack side plates 11. The second rotary valve 5b is arranged through the rack side plates 11, and the liquid path interface on the second rotary valve 5b is located on the side of the rack side plates 11 facing the sequencing chip 7.

[0058] In one embodiment, please refer to Figure 1 and Figure 5 The chip moving platform 2 includes a moving seat 22, which is arranged on the frame 1 for movement along a first direction, and a chip seat 21 is arranged on the moving seat 22 for movement along a second direction, so that the chip seat 21 moves along the first direction and the second direction. In one embodiment, the moving seat 22 and the chip seat 21 form a cross slide.

[0059] The chip holder driving mechanism includes a first direction driving mechanism and a second direction driving mechanism, wherein the first direction driving mechanism drives the movable holder 22 to move in the first direction, and the second direction driving mechanism drives the chip holder 21 to move in the second direction. Both the first direction driving mechanism and the second direction driving mechanism can adopt a gear rack driving mechanism, a lead screw nut driving mechanism, a linear motor or a driving cylinder.

[0060] It should be noted that in the present application, the chip seat 21 is configured on the movable seat 22. The movable seat 22 can drive the chip seat 21 to move while moving. Therefore, when the movable seat 22 moves relative to the frame 1, the chip seat 21 also moves relative to the frame 1. When the chip seat 21 moves relative to the movable seat 22, it also moves relative to the frame 1.

[0061] The sequencing channel 71 on the sequencing chip 7 extends along the first direction. During the process of photographing the sequencing channel 71, the sequencing chip 7 needs to move in the first direction, and the sequencing chip 7 also needs to move in the second direction. During the sequencing process of the sequencing chip 7, the moving distance of the moving seat 22 in the first direction is greater than the moving distance of the chip seat 21 in the second direction, and the rotary valve seat 3 can only reciprocate along the first direction. This can simplify the structure of the movement of the rotary seat, and preferentially allow the rotary seat to follow the movement of the chip seat 21 in the first direction. Of course, in one embodiment, the chip seat 21 can also only move relative to the frame 1 in the first direction. In addition to being able to move relative to the frame 1 in the first direction, the rotary valve seat 3 can also move relative to the frame 1 in the second direction.

[0062] In one embodiment, please refer to Figure 1 and Figure 2 The valve seat driving mechanism 4 is a pulley driving mechanism, which includes a driving wheel 41, a driven wheel 42 and a transmission belt 43. The driving wheel 41 and the driven wheel 42 are arranged at intervals along the first direction, and the transmission belt 43 is wound around the driving wheel 41 and the driven wheel 42. The rotary valve seat 3 is connected to the transmission belt 43, so that the driving wheel 41 can drive the rotary valve seat 3 to move through the transmission belt 43. The pulley driving mechanism also includes a driving motor 44, which is fixed on the frame 1, the driving wheel 41 is fixed on the output shaft of the driving motor 44, and the driven wheel 42 is rotatably configured on the frame 1.

[0063] To facilitate the installation of the valve seat drive mechanism 4, please refer to Figure 1 and Figure 2 The frame 1 includes a column 12 and a longitudinal beam 13 fixed on the column 12, the longitudinal beam 13 extends along a first direction, and the rotary valve seat 3 is movably arranged on a portion of the longitudinal beam 13 overhanging on one side of the column 12. By hanging the rotary valve seat 3 on one side of the column 12, the column 12 can be arranged at a position far away from the chip seat 21, which is convenient for the installation of the rotary valve seat 3 and also makes the overall space layout more compact. The drive motor 44 and the rotary valve seat 3 are respectively located on opposite sides of the column 12 so that the two sides of the column 12 can be balanced.

[0064] Please refer to Figure 1 , a guide rail 14 is fixed on the longitudinal beam 13, and the guide rail 14 extends in the first direction. The rotary valve seat 3 cooperates with the guide rail 14 in the first direction, so that the guide rail 14 guides the rotary valve seat 3 to move. The number of columns 12 and longitudinal beams 13 is two, and correspondingly, each longitudinal beam 13 is provided with a guide rail 14. After the rotary valve seat 3 is installed on the longitudinal beam 13, a gantry structure is formed. The frame side plate 11 is on the outside of the two columns 12. The rotary valve seat 3 includes a cross beam 31 extending in the second direction and a fixing frame 32 fixed to one side of the cross beam 31, and the first rotary valve 5a is fixed on the fixing frame 32. In one embodiment, please refer to Figure 1 , the second rotary valve 5b is also fixed on the fixing frame 32. In one embodiment, please refer to Figure 5 The first rotary valve 5 a is fixed on the fixing frame 32 , and the second rotary valve 5 b is fixed on the frame 1 .

[0065] In one embodiment, in addition to the pulley drive mechanism, the valve seat drive mechanism 4 may also adopt a gear rack drive mechanism, a screw nut drive mechanism, a linear motor, a drive cylinder, etc.

[0066] In an embodiment of a mobile liquid supply device of the present application, please refer to Figures 1 to 6The mobile liquid supply device includes a frame 1, a first rotary valve 5a, a rotary valve seat 3 and a valve seat driving mechanism 4. The first rotary valve 5a is used to select a target sample and / or a target reagent and inject it into the sequencing flow channel 71 of the sequencing chip 7. The rotary valve seat 3 is movably configured on the frame 1, and the first rotary valve 5a is configured on the rotary valve seat 3. The valve seat driving mechanism 4 is used to drive the rotary valve seat 3 to move so that the first rotary valve 5a can move with the sequencing chip 7. The structures of the frame 1, the first rotary valve 5a, the rotary valve seat 3 and the valve seat driving mechanism 4 are the same as those described in any of the above embodiments, and will not be described in detail.

[0067] Furthermore, in one embodiment, the mobile liquid supply device includes a second rotary valve 5b. Part of the liquid inlet of the first rotary valve 5a is connected to the second rotary valve 5b, so that the second rotary valve 5b supplies liquid to the first rotary valve 5a. The second rotary valve 5b is disposed on the rotary valve seat 3.

[0068] The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. For those skilled in the art of the present invention, some simple deductions, deformations or substitutions can be made based on the idea of ​​the present invention.

Claims

1. A gene sequencer, characterized in that: include: frame; A chip motion platform, the chip motion platform comprising a chip seat and a chip seat driving mechanism, the chip seat is used to mount a sequencing chip, the chip seat is movably configured on the frame, and the chip seat driving mechanism is used to drive the chip seat to move; A liquid circuit system, the liquid circuit system is connected to the sequencing channel of the sequencing chip; the liquid circuit system includes a first rotary valve, the first rotary valve is used to select a target sample and / or a target reagent to be injected into the sequencing channel; the liquid circuit system also includes a chip liquid supply pipeline, the chip liquid supply pipeline is used to be connected between the first rotary valve and the sequencing chip; A rotary valve seat, the rotary valve seat is movably arranged on the frame, and the first rotary valve is arranged on the rotary valve seat; A valve seat driving mechanism, the valve seat driving mechanism is used to drive the rotary valve seat to move; And a control system, wherein the control system is connected to the valve seat driving mechanism and the chip seat driving mechanism, and is used to control the rotary valve seat to follow the movement of the chip seat.

2. The gene sequencer according to claim 1, characterized in that: The liquid circuit system includes a second rotary valve; at least part of the liquid inlet of the first rotary valve is connected to the second rotary valve, so that the second rotary valve can supply liquid to the first rotary valve; the second rotary valve is arranged on the rotary valve seat.

3. The gene sequencer according to claim 2, characterized in that: The rotary valve seat is movably arranged on the frame along a first direction, the first rotary valve and the second rotary valve are arranged side by side in a second direction, and an angle between the first direction and the second direction is greater than 0 degree and not greater than 90 degrees.

4. The gene sequencer according to claim 2, characterized in that: The rotary valve seat is movably configured on the frame along a first direction, the first rotary valve is in the shape of an elongated strip extending along the first direction, the second rotary valve is in the shape of an elongated strip extending along a second direction, and the angle between the first direction and the second direction is greater than 0 degrees and not greater than 90 degrees; the liquid path interface of the first rotary valve is located at one end of the first rotary valve that is used to be close to the sequencing chip.

5. The gene sequencer according to claim 1, characterized in that: The liquid circuit system includes a second rotary valve; part of the liquid inlet of the first rotary valve is connected to the second rotary valve, so that the second rotary valve can supply liquid to the first rotary valve; The second rotary valve is configured on the frame.

6. The gene sequencer according to claim 1 or 2, characterized in that: The rotary valve seat is configured on the frame for movement along a first direction, the control system can control the rotary valve seat to follow the chip seat to move in the first direction, the chip movement platform includes a moving seat, the moving seat is configured on the frame for movement along a first direction, and the chip seat is configured on the moving seat for movement along a second direction, so that the chip seat moves along the first direction and the second direction.

7. The gene sequencer according to claim 6, characterized in that: The first direction is used to be consistent with the extension direction of the sequencing channel. During the sequencing process of the sequencing chip, the moving distance of the movable seat in the first direction is greater than the moving distance of the chip seat in the second direction, and the rotary valve seat can only reciprocate along the first direction.

8. The gene sequencer according to claim 6, characterized in that: The valve seat driving mechanism includes a driving wheel, a driven wheel and a transmission belt. The driving wheel and the driven wheel are arranged at intervals along the first direction. The transmission belt is wound around the driving wheel and the driven wheel. The rotary valve seat is connected to the transmission belt so that the driving wheel can drive the rotary valve seat to move through the transmission belt.

9. The gene sequencer according to claim 6, characterized in that: The frame includes a column and a longitudinal beam fixed on the column, the longitudinal beam extends along the first direction, and the rotary valve seat is movably arranged on a portion of the longitudinal beam overhanging one side of the column.

10. A mobile liquid supply device, characterized in that: include: frame; A first rotary valve, wherein the first rotary valve is used to select a target sample and / or a target reagent and inject it into a sequencing flow channel of a sequencing chip; A rotary valve seat, the rotary valve seat is movably arranged on the frame, and the first rotary valve is arranged on the rotary valve seat; and a valve seat driving mechanism, used for driving the rotary valve seat to move so that the first rotary valve can follow the movement of the sequencing chip.

11. The mobile liquid supply device according to claim 10, characterized in that: The mobile liquid supply device comprises a second rotary valve; part of the liquid inlet of the first rotary valve is connected to the second rotary valve so that the second rotary valve can supply liquid to the first rotary valve; the second rotary valve is arranged on the rotary valve seat.

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